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https://www.readbyqxmd.com/read/30110167/complete-genome-of-micromonospora-sp-strain-b006-reveals-biosynthetic-potential-of-a-lake-michigan-actinomycete
#1
Jana Braesel, Camila M Crnkovic, Kevin J Kunstman, Stefan J Green, Mark Maienschein-Cline, Jimmy Orjala, Brian T Murphy, Alessandra S Eustáquio
Actinomycete bacteria isolated from freshwater environments are an unexplored source of natural products. Here we report the complete genome of the Great Lakes-derived Micromonospora sp. strain B006, revealing its potential for natural product biosynthesis. The 7-megabase pair chromosome of strain B006 was sequenced using Illumina and Oxford Nanopore technologies followed by Sanger sequencing to close remaining gaps. All identified biosynthetic gene clusters (BGCs) were manually curated. Five known BGCs were identified encoding desferrioxamine, alkyl- O-dihydrogeranylmethoxyhydroquinone, a spore pigment, sioxanthin, and diazepinomicin, which is currently in phase II clinical trials to treat Phelan-McDermid syndrome and co-morbid epilepsy...
August 15, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/30107533/syt1-associated-neurodevelopmental-disorder-a-case-series
#2
Kate Baker, Sarah L Gordon, Holly Melland, Fabian Bumbak, Daniel J Scott, Tess J Jiang, David Owen, Bradley J Turner, Stewart G Boyd, Mari Rossi, Mohammed Al-Raqad, Orly Elpeleg, Dawn Peck, Grazia M S Mancini, Martina Wilke, Marcella Zollino, Giuseppe Marangi, Heike Weigand, Ingo Borggraefe, Tobias Haack, Zornitza Stark, Simon Sadedin, Tiong Yang Tan, Yunyun Jiang, Richard A Gibbs, Sara Ellingwood, Michelle Amaral, Whitley Kelley, Manju A Kurian, Michael A Cousin, F Lucy Raymond
Synaptotagmin 1 (SYT1) is a critical mediator of fast, synchronous, calcium-dependent neurotransmitter release and also modulates synaptic vesicle endocytosis. This paper describes 11 patients with de novo heterozygous missense mutations in SYT1. All mutations alter highly conserved residues, and cluster in two regions of the SYT1 C2B domain at positions Met303 (M303K), Asp304 (D304G), Asp366 (D366E), Ile368 (I368T) and Asn371 (N371K). Phenotypic features include infantile hypotonia, congenital ophthalmic abnormalities, childhood-onset hyperkinetic movement disorders, motor stereotypies, and developmental delay varying in severity from moderate to profound...
August 13, 2018: Brain: a Journal of Neurology
https://www.readbyqxmd.com/read/30106954/neuroinflammation-and-er-stress-are-key-mechanisms-of-acute-bilirubin-toxicity-and-hearing-loss-in-a-mouse-model
#3
Emanuele Schiavon, Joshua L Smalley, Sherylanne Newton, Nigel H Greig, Ian D Forsythe
Hyperbilirubinemia (jaundice) is caused by raised levels of unconjugated bilirubin in the blood. When severe, susceptible brain regions including the cerebellum and auditory brainstem are damaged causing neurological sequelae such as ataxia, hearing loss and kernicterus. The mechanism(s) by which bilirubin exerts its toxic effect have not been completely understood to date. In this study we investigated the acute mechanisms by which bilirubin causes the neurotoxicity that contributes to hearing loss. We developed a novel mouse model that exhibits the neurological features seen in human Bilirubin-Induced Neurological Dysfunction (BIND) syndrome that we assessed with a behavioural score and auditory brainstem responses (ABR)...
2018: PloS One
https://www.readbyqxmd.com/read/30106181/mesenchymal-cox2-pg-secretome-engages-nr4a-wnt-signalling-axis-in-haematopoietic-progenitors-to-suppress-anti-leukaemia-immunity
#4
Limei Wu, Surya Amarachintha, Jian Xu, Frank Oley, Wei Du
The bone marrow (BM) microenvironment (niche) plays important roles in supporting normal/abnormal haematopoiesis. We investigated the interaction between leukaemic mesenchymal niche and haematopoietic stem and progenitor cells (HSPCs) using the model of Fanconi anaemia (FA), a genetic disorder characterized by BM failure and leukaemia. Healthy donor HSPCs co-cultured on mesenchymal stromal cells (MSCs) derived from FA patients with acute myeloid leukaemia (AML) exhibited higher human engraftment and myeloid expansion in Non-obese diabetic severe combined immunodeficiency IL-2γ-/- /SGM3 recipients...
August 14, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/30104323/beta-3-adrenoceptor-signaling-pathways-in-urothelial-and-smooth-muscle-cells-in-the-presence-of-succinate
#5
Abubakr H Mossa, Monica Velasquez Flores, Hieu Nguyen, Philippe Cammisotto, Lysanne Campeau
Succinate, an intermediate metabolite of the Krebs cycle, can alter the metabolomics response to certain drugs, and controls an array of molecular responses in the urothelium through activation of its receptor GPR91. Mirabegron, a β3-adrenergic receptor (β3-AR) agonist used to treat overactive bladder syndrome, increases intracellular cyclic AMP (cAMP) in the detrusor smooth muscle cells (SMCs), leading to relaxation. We have previously shown that succinate inhibits forskolin-stimulated cAMP production in urothelium...
August 13, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/30103195/evaluation-of-a-lymphocyte-transformation-test-and-cytokine-detection-assay-to-identify-phenytoin-and-carbamazepine-provoked-dress-or-sjs-ten-in-epilepsy-patients
#6
Pongsathorn Kumkamthornkul, Somkiat Udnaen, Tunsuda Tansit, Papapit Tuchinda, Yuttana Srinoulprasert
Phenytoin (PHE) and carbamazepine (CBZ) are first rank causative drugs that can induce drug rash with eosinophilia and systemic symptoms (DRESS) and Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN). Identification of anti-epileptic drugs as a culprit drug has been problematic; hence, in vitro tests could be promising methods to define causative drugs without clinical risk. The aim of this study is to evaluate the efficacy of lymphocyte transformation tests (LTT) and cytokine detection assays in identifying PHE and CBZ as culprit drugs...
August 10, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/30102951/angioinvasive-fungal-infections-impacting-the-skin-background-epidemiology-and-clinical-presentation-part-1
#7
REVIEW
Bridget E Shields, Misha Rosenbach, Zoe Brown-Joel, Anthony Berger, Bradley A Ford, Karolyn A Wanat
Angioinvasive fungal infections cause significant morbidity and mortality due to their propensity to invade blood vessel walls, resulting in catastrophic tissue ischemia, infarct, and necrosis. While occasionally seen in immunocompetent hosts, opportunistic fungi are emerging in immunosuppressed hosts, including patients with hematologic malignancy, acquired immunodeficiency syndrome (AIDS), organ transplant, and poorly controlled diabetes mellitus. The widespread use of antifungal prophylaxis has led to an "arms race" of emerging fungal resistance patterns...
August 10, 2018: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/30102264/isolation-of-atrial-cardiomyocytes-from-a-rat-model-of-metabolic-syndrome-related-heart-failure-with-preserved-ejection-fraction
#8
David Bode, Tim Guthof, Burkert M Pieske, Frank R Heinzel, Felix Hohendanner
In this article, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes (ACMs) from a rat model of metabolic syndrome (MetS)-related heart failure with preserved ejection fraction (HFpEF). The prevalence of MetS-related HFpEF is rising, and atrial cardiomyopathies associated with atrial remodeling and atrial fibrillation are clinically highly relevant as atrial remodeling is an independent predictor of mortality. Studies with isolated single-cell cardiomyocytes are frequently used to corroborate and complement in vivo findings...
July 26, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/30096918/the-antifungal-properties-of-epidermal-fatty-acid-esters-insights-from-white-nose-syndrome-wns-in-bats
#9
Craig L Frank, Katherine G Sitler-Elbel, Anna J Hudson, Melissa R Ingala
Numerous free fatty acids (FFAs) are known to have potent antifungal effects. The mammalian epidermis contains both FFAs and multiple classes of fatty acid esters, including 1-monoacylglycerols and wax esters. We thus hypothesized that wax esters and 1-monoacylglycerols composed of antifungal fatty acids would also have antifungal properties. We tested this hypothesis by examining the effects of 1-monoacylglycerols, 1,3-diacylglycerols, and wax esters on the growth of Pseudogymnoascus destructans ( Pd ), the fungus that causes White-nose Syndrome (WNS) in North American bats by invading their epidermis...
August 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30096796/exploring-the-impact-of-the-biofloc-rearing-system-and-an-oral-wssv-challenge-on-the-intestinal-bacteriome-of-litopenaeus-vannamei
#10
Mariana R Pilotto, André N A Goncalves, Felipe N Vieira, Walter Q Seifert, Evelyne Bachère, Rafael D Rosa, Luciane M Perazzolo
We provide a global overview of the intestinal bacteriome of Litopenaeus vannamei in two rearing systems and after an oral challenge by the White spot syndrome virus (WSSV). By using a high-throughput 16S rRNA gene sequencing technology, we identified and compared the composition and abundance of bacterial communities from the midgut of shrimp reared in the super-intensive biofloc technology (BFT) and clear seawater system (CWS). The predominant bacterial group belonged to the phylum Proteobacteria, followed by the phyla Bacteroidetes, Actinobacteria, and Firmicutes...
August 8, 2018: Microorganisms
https://www.readbyqxmd.com/read/30095246/novel-scaffolds-for-dual-specificity-tyrosine-phosphorylation-regulated-kinase-dyrk1a-inhibitors
#11
Anna Czarna, Jinhua Wang, Diana Zelencova, Yao Liu, Xianming Deng, Hwan Geun Choi, Tinghu Zhang, Wenjun Zhou, Jae Won Chang, Hanne Kildalsen, Ole-Morten Seternes, Nathanael S Gray, Richard Alan Engh, Ulli Rothweiler
DYRK1A is one of five members of the Dual-specificity tyrosine (Y) phosphorylation-Regulated Kinase (DYRK) family. The DYRK1A gene is located in the Down syndrome critical region and regulates cellular processes related to proliferation and differentiation of neuronal progenitor cells during early development. This has focused research on to its role in neuronal degenerative diseases, including Alzheimer's and Down syndrome; recent studies have also shown a possible role of DYRK1A in diabetes. Here we report a variety of scaffolds not generally known for DYRK1A inhibition, demonstrating their effects in in vitro assays and also in cell cultures...
August 10, 2018: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/30094378/llc-tumor-cells-derivated-factors-reduces-adipogenesis-in-co-culture-system
#12
Magno Alves Lopes, Felipe Oliveira Franco, Felipe Henriques, Sidney Barnabé Peres, Miguel Luiz Batista
Cancer cachexia (CC) is a multifactorial syndrome with an unknown etiology. The primary symptom is the progressive reduction of the body weight. Recently, down-regulation of adipogenic and lipogenic genes were demonstrated to be early affected during cachexia progression in adipose tissue (AT), resulting in AT remodeling. Thus, this study aimed to evaluate in a co-culture system the influence of the Lewis Lung Carcinoma (LLC) tumor cells (c/c-LLC) in an established pre-adipocyte cell line 3T3-L1 adipogenic capacity...
July 2018: Heliyon
https://www.readbyqxmd.com/read/30091999/aryl-hydrocarbon-receptor-ahr-is-a-novel-druggable-pathway-controlling-malignant-progenitor-proliferation-in-chronic-myeloid-leukemia-cml
#13
Melanie Gentil, Patricia Hugues, Christophe Desterke, Gladys Telliam, Ivan Sloma, Lucas E B Souza, Seda Baykal, Jerome Artus, Frank Griscelli, Agnes Guerci, Hyacinthe Johnson-Ansah, Adlen Foudi, Annelise Bennaceur-Griscelli, Ali G Turhan
Aryl Hydrocarbon Receptor (AHR) is an ubiquitous basic helix-loop-helix transcription factor, which is ligand-activated and involved in numerous biological processes including cell division, cell quiescence and inflammation. It has been shown that AHR is involved in normal hematopoietic progenitor proliferation in human cells. In addition, loss of AHR in knockout mice is accompanied by a myeloproliferative syndrome-like disease, suggesting a role of AHR in hematopoietic stem cell (HSC) maintenance. To study the potential role of AHR pathway in CML progenitors and stem cells, we have first evaluated the expression of AHR in UT-7 cell line expressing BCR-ABL...
2018: PloS One
https://www.readbyqxmd.com/read/30091957/validation-of-oral-health-impact-profile-14-and-its-association-with-hypossialia-in-a-sj%C3%A3-gren-syndrome-portuguese-population
#14
João Amaral, Catarina Sanches, Duarte Marques, José Vaz Patto, Filipe Barcelos, António Mata
AIMS: The objective of this study was to perform the Portuguese transcultural adaptation of the original Brazilian version of the Oral Health Impact Profile-14 (OHIP-14) while evaluating the association between hyposalivation and quality of life in a Sjögren´s Syndrome population. METHODS: The original Brazilian version of the Oral Health Impact Profile-14 was culturally adapted following the guidelines for cross-cultural adaptation of health-related quality of life measures...
April 2018: Acta Reumatológica Portuguesa
https://www.readbyqxmd.com/read/30090776/biopsychosocial-etiology-of-premenstrual-syndrome-a-narrative-review
#15
Mojgan Zendehdel, Forouzan Elyasi
Introduction: Premenstrual syndrome (PMS) is one of the most prevalent disorders at reproductive age and has a negative impact on emotions and performance of women. Since various factors play a role in the development of this syndrome, the present study was aimed to examine biopsychosocial etiology of PMS in the form of a narrative review. Materials and Methods: Relevant studies were collected based on the three subjects of biological, psychological, and social etiologies during 1987-2015...
March 2018: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/30089518/trichoderma-polysporum-selectively-inhibits-white-nose-syndrome-fungal-pathogen-pseudogymnoascus-destructans-amidst-soil-microbes
#16
Amanpreet Singh, Erica Lasek-Nesselquist, Vishnu Chaturvedi, Sudha Chaturvedi
BACKGROUND: Pseudogymnoascus destructans (Pd), the causative fungal agent of white-nose syndrome (WNS), has led to the deaths of millions of hibernating bats in the United States of America (USA) and Canada. Efficient strategies are needed to decontaminate Pd from the bat hibernacula to interrupt the disease transmission cycle without affecting the native microbes. Previously, we discovered a novel Trichoderma polysporum (Tp) strain (WPM 39143), which inhibited the growth of Pd in autoclaved soil samples...
August 8, 2018: Microbiome
https://www.readbyqxmd.com/read/30088950/making-ivf-more-effective-through-the-evolution-of-prediction-models-is-prognosis-the-missing-piece-of-the-puzzle
#17
Mara Simopoulou, Konstantinos Sfakianoudis, Nikolaos Antoniou, Evangelos Maziotis, Anna Rapani, Panagiotis Bakas, George Anifandis, Theodoros Kalampokas, Stamatis Bolaris, Agni Pantou, Konstantinos Pantos, Michael Koutsilieris
Assisted reproductive technology has evolved tremendously since the emergence of in vitro fertilization (IVF). In the course of the recent decade, there have been significant efforts in order to minimize multiple gestations, while improving percentages of singleton pregnancies and offering individualized services in IVF, in line with the trend of personalized medicine. Patients as well as clinicians and the entire IVF team benefit majorly from 'knowing what to expect' from an IVF cycle. Hereby, the question that has emerged is to what extent prognosis could facilitate toward the achievement of the above goal...
August 8, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/30088215/putative-neuromycotoxicoses-in-an-adult-male-following-ingestion-of-moldy-walnuts
#18
C J Botha, C M Visagie, M Sulyok
A tremorgenic syndrome occurs in dogs following ingestion of moldy walnuts, and Penicillium crustosum has been implicated as the offending fungus. This is the first report of suspected moldy walnut toxicosis in man. An adult male ingested approximately eight fungal-infected walnut kernels and after 12 h experienced tremors, generalized pain, incoordination, confusion, anxiety, and diaphoresis. Following symptomatic and supportive treatment at a local hospital, the man made an uneventful recovery. A batch of walnuts (approximately 20) was submitted for mycological culturing and identification as well as for mycotoxin analysis...
August 7, 2018: Mycotoxin Research
https://www.readbyqxmd.com/read/30087164/advancing-quality-in-sepsis-management-a-large-scale-programme-for-improving-sepsis-recognition-and-management-in-the-north-west-region-of-england
#19
Emmanuel Fru Nsutebu, Ana Belén Ibarz-Pavón, Elizabeth Kanwar, Nancy Prospero, Neil French, Conor McGrath
OBJECTIVE: To evaluate the impact of a collaborative programme for the early recognition and management of patients admitted with sepsis in the northwest of England. SETTING: 14 hospitals in the northwest of England. INTERVENTION: A quality improvement programme (Advancing Quality (AQ) Sepsis) that promoted a sepsis care bundle including time-based recording of early warning scores, documenting systemic inflammatory response syndrome criteria and suspected source of infection, taking of blood cultures, measuring serum lactate levels, administration of intravenous antibiotics, administration of oxygen, fluid resuscitation, measurement of fluid balance and senior review...
August 7, 2018: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/30086713/bilateral-osteomyelitis-and-liver-abscess-caused-by-hypervirulent-klebsiella-pneumoniae-a-rare-clinical-manifestation-case-report
#20
Emma Sturm, Alex Tai, Belinda Lin, Jason Kwong, Eugene Athan, Benjamin P Howden, Richard D Angliss, Rafik Asaid, James Pollard
BACKGROUND: Hypervirulent strains of Klebsiella pneumoniae are a recognized cause of a distinct invasive syndrome that results in pyogenic liver abscesses and metastatic complications, particularly in the Asia Pacific region. Reports of hypervirulent K.pneumoniae in Europe, the Americas and Australia indicate worldwide spread. We present a case of multi-focal osteomyelitis, a rarely described complication of hypervirulent K.pneumoniae in the medical literature. The prevalence of this condition in countries outside Asia may be expected to rise with increasing travel...
August 7, 2018: BMC Infectious Diseases
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