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E W Nabofa, A R A Alada
Previous studies on the ability of caffeine to enhance endurance and boost performance have focused on theenergy substrates that are utilized by the skeletal muscle and the brain but nothing of such has been reported on cardiactissue. This study was designed to investigate the effect of caffeine on cardiac tissue metabolism in the rabbit. The study wascarried out on adult male New Zealand rabbits divided into 3 groups (n=5). Group I rabbits served as control and were given0.5ml/Kg of normal saline while group II and III rabbits were administered with 2mg/Kg and 6mg/kg of caffeine respectivelyfor 28 days...
June 30, 2018: Nigerian Journal of Physiological Sciences: Official Publication of the Physiological Society of Nigeria
Gregory T Sigurdson, Rebecca J Robbins, Thomas M Collins, M Mónica Giusti
Anthocyanins express many hues depending on environmental factors and structural aspects, of which aglycone structure and acylation have been considered most impactful. Effects of different glycosylations on anthocyanins' colors are less known. Twelve cyanidin and 3-deoxy-cyanidin (luteolinidin) derivatives were isolated from red cabbage and black carrot hydrolysates and from black sorghum, diluted in pH 1-9, and analyzed by spectrophotometry and colorimetry. Location, number, and structure of glycosylations affected λmax and spectral shape of o-dihydroxylated anthocyanins, playing important roles on color expression...
December 1, 2018: Food Chemistry
Uilson Pereira Dos Santos, Georgina S Tolentino, Jorge Sá Morais, Kely de Picoli Souza, Leticia M Estevinho, Edson Lucas Dos Santos
Hancornia speciosa Gomes is a fruit tree, commonly known as the mangaba tree, which is widespread throughout Brazil. The leaves of this plant are used in traditional medicine for medicinal purposes. Thus, the objective of this study was to perform a physicochemical characterization, identify the lipophilic antioxidants and fatty acids, and determine the microbiological quality and safety of H. speciosa leaves. In addition, the antioxidant, antimutagenic, and inhibitory activities of the ethanolic extract of H...
2018: Oxidative Medicine and Cellular Longevity
Piet E J van Erp, Malou Peppelman, Denise Falcone
In this review we aim to give a concise and selective overview of noninvasive biophysical analysis techniques for skin barrier analysis (transepidermal water loss, electrical methods, confocal Raman microspectroscopy, sebumeter, reflectance spectrophotometry, tristimulus colorimetry, diffuse reflectance spectroscopy and reflectance confocal microscopy), including advantages and limitations. Rather than giving an exhaustive description of the many techniques currently available, we show the usefulness of a representative selection of techniques in the functional and morphological evaluation of the skin barrier...
July 17, 2018: Experimental Dermatology
Jiaxuan He, Jianfang Zhu
BACKGROUND Collapsin response mediator protein-2 (CRMP-2) is the first member of the CRMP family that has been identified in primary neuronal cells; it was originally found and identified in the regulation of microtubule dimerization into microtubules. MATERIAL AND METHODS In the present study, we aimed to investigate the roles and mechanisms of CRMP-2 in sevoflurane-induced neurocyte injury. Cell viability, proliferation, and apoptosis were measured by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. Colorimetry was performed to measure the activity of caspase-3...
July 18, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Ziguan Zhu, Qingping Xie, Yazeng Huang, Shuijun Zhang, Yu Chen
Aucubin represents an iridoid glucoside separated from multiple Chinese herbs, which has been demonstrated to possess numerous pharmacological activities. In the present study, the aim was to investigate the roles and mechanisms of aucubin in the suppression of mouse MC3T3‑E1 osteoblast apoptosis induced by Titanium particles and the promotion of bone formation. MTT assay and flow cytometry were performed to analyze cell viability and apoptosis, respectively. ELISA and para‑nitrophenyl phosphate colorimetry were carried out to evaluate the oxidative stress markers and alkaline phosphatase (ALP)...
September 2018: Molecular Medicine Reports
Junli He, Guiyue Shen, Anding Liu, Xiaojing Jiang
OBJECTIVE: To investigate the effects of nicotinamide phosphoribosyl transferase (NAMPT) inhibitor FK866 on polymicrobial sepsis-induced liver injury in mice. METHODS: Eighty-four healthy male C57BL/6J mice were divided into four groups by random number table method (n = 21): sham group, sepsis-induced liver injury model by cecal ligation and perforation group (CLP group), vehicle+CLP group and FK866+CLP group. FK866 (10 mg/kg) or same volume dimethyl sulfoxide were given intraperitoneally into mice 24, 12 and 0...
June 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Shadab Faham, Gholamreza Khayatian, Hamed Golmohammadi, Raouf Ghavami
A paper based analytical device is presented for the determination of Cr(III) and Cr(VI) using gold nanoparticles (AuNPs) modified with 2,2'-thiodiacetic acid. The modified AuNPs were characterized using UV-Vis spectrophotometry, Fourier transform infrared, dynamic light scattering, zeta potential, energy dispersive spectroscopy and transmission electron microscopy. Cr(III) ions induce the aggregation of the modified AuNPs, and the color of the nanoprobe changes from red to blue. This can be detected visually, or by colorimetry, or with a camera...
July 13, 2018: Mikrochimica Acta
Jin-Ho Lee, Hyeon-Yeol Cho, Hye Kyu Choi, Ji-Young Lee, Jeong-Woo Choi
Gold nanoparticles (GNPs) have been widely utilized to develop various biosensors for molecular diagnosis, as they can be easily functionalized and exhibit unique optical properties explained by plasmonic effects. These unique optical properties of GNPs allow the expression of an intense color under light that can be tuned by altering their size, shape, composition, and coupling with other plasmonic nanoparticles. Additionally, they can also enhance other optical signals, such as fluorescence and Raman scattering, making them suitable for biosensor development...
July 11, 2018: International Journal of Molecular Sciences
Jie Wei, Weidan Chang, Aori Qileng, Weipeng Liu, Yue Zhang, Shiya Rong, Hongtao Lei, Yingju Liu
Microcystins, the lethal cyanotoxins from Microcystis aeruginosa, can inhibit the activity of protein phosphatase and promote liver tumors. Herein, a dual-modal split-type immunosensor was constructed to detect microcystin-LR (MC-LR), based on the photocurrent change of CdS/ZnO hollow nanorod arrays (HNRs) and the blue shift of the surface plasmon resonance peak from Au nanobipyramids@Ag. By using mesoporous silica nanospheres as the carrier to immobilize secondary antibody and DNA primer, a hybridization chain reaction was adopted to capture alkaline phosphatase, while its catalytic reaction product, ascorbic acid, exhibited dual functions...
July 20, 2018: Analytical Chemistry
Mahsa Karshenas, Mohammad Goli, Nafiseh Zamindar
Egg yolk was replaced with sesame-peanut meal milk in mayonnaise in the levels of 0, 25%, 50%, 75%, and 100%. The pH was significantly decreased by increasing the percentage of replacement in all three kinds of replacement ( p  < .05). However, over the whole period, no significant difference was observed in the acidity. The mayonnaise samples, except for the replacements of 50%, were desirable in terms of physical and thermal stability. A significant decrease was seen in lightness ( L *) and yellowness ( b *) of the samples as a result of an increase in the percentage of replacements ( p  < ...
June 2018: Food Science & Nutrition
Z Y Wang, X Q Wang, Y K Liu, B Yuan, J Y Dong, F Song, Y Z Jiang, S L Lu
Objective: To investigate the effects of denatured collagen type Ⅰ on differentiation of human fibroblasts into myofibroblasts. Methods: A small amount of normal skin donated by burn patients undergoing scar surgery was collected. Human fibroblasts were obtained by method of explant culture and then sub-cultured. The fourth passage of cells were used in the following experiments. (1) Fibroblasts were divided into normal collagen group and denatured collagen group according to the random number table, with 10 wells in each group...
February 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
Bevil R Conway, Rhea T Eskew, Paul R Martin, Andrew Stockman
The study of color vision encompasses many disciplines, including art, biochemistry, biophysics, brain imaging, cognitive neuroscience, color preferences, colorimetry, computer modelling, design, electrophysiology, language and cognition, molecular genetics, neuroscience, physiological optics, psychophysics and physiological optics. Coupled with the elusive nature of the subjective experience of color, this wide range of disciplines makes the study of color as challenging as it is fascinating. This overview of the special issue Color: Cone Opponency and Beyond outlines the state of the science of color, and points to some of the many questions that remain to be answered in this exciting field...
June 27, 2018: Vision Research
F F Li, D Sha, X Y Qin, C Z Li, B Lin
We aimed to investigate the role of FUT1 gene in Taxol resistance and to explore its mechanism in epithelial ovarian cancer. Three ovarian cancer cell lines, ES-2, SK-OV-3 and OVCAR-3 were selected from epithelial ovarian cancer in this experiment. Western blot was used to validate the protein expression level of FUT1 and the apoptosis proteins. The expression level of the corresponding carrier was validated by RT-PCR. Transfection and isolation of stable transfectants were carried out to establish the cell line models...
June 26, 2018: Neoplasma
Stella A Ordoudi, Chrysoula Staikidou, Anastasia Kyriakoudi, Maria Z Tsimidou
The stepwise approach takes advantage of simple, versatile, low-cost screening tools that can be applied to several posts of the saffron trade chain to specifically detect adulteration with carminic acid (CA). This natural dye is of insect origin and should not be present in Kosher and Halal foods such as saffron. For gross adulteration levels (>25.0%, w/w) reaction with diphenylamine-sulfuric acid was found adequate to indicate the presence of extraneous matter but not its identity. FT-IR analysis of the dry material combined with chemometrics served to rapidly sort out samples containing >10...
November 30, 2018: Food Chemistry
Patricia Lopez, Jon Mabe, Guillermo Miró, Leire Etxeberria
Lubricant and hydraulic fluid ageing impacts the performance of the machines, gears, transmissions or automatisms where they are being used. This manuscript describes the work accomplished for bringing an innovative measurement concept for analysing the physical- chemical properties of these fluids, to a real industrial product ready to be integrated into different industrial equipment. The steps taken to deal with uncertainties and evolving requirements while progressing in the sensor development are described, covering the stages of theoretical formulation of the problem, optical and fluidic simulations, sensor prototype development and tests...
June 22, 2018: Sensors
Mozhgan Aghajanzadeh, Mostafa Zamani, Hamid Rashidzadeh, Kobra Rostamizadeh, Ali Sharafi, Hossein Danafar
In this project, a core-shell Polymersome based on miktoarm star-copolymer: methoxy Poly Ethylene Glycol-Lysine-(Poly Caprolactone)2 (PEG-Lys-PCL2 ) was synthesized by a new method as controlled targeted drug delivery systems for co-delivery of the chemotherapeutic methotrexate (MTX) and curcumin (CUR). Some properties of these nano carriers (NCs) such as surface morphology, structure, surface charge, stability and biocompatibility were evaluated by Proton nuclear magnetic resonance (1 HNMR), dynamic scanning colorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), Dynamic light scattering (DLS), atomic force microscopy (AFM), Critical aggregation concentration (CAC), hemolysis test, MTT assay and lethal dose 50 (LD50)...
June 16, 2018: Journal of Biomedical Materials Research. Part A
Adela Ngwewondo, Meng Wang, Faustin Pascal T Manfo, Moses Samje, Jessie N'kam Ganin's, Emmanuel Ndi, Raymond J Andersen, Fidelis Cho-Ngwa
BACKGROUND: Ivermectin is the only drug currently recommended for the treatment of onchocerciasis, the second leading infectious cause of blindness in the world. This drug kills only the first stage larvae-microfilariae (mf) of Onchocerca volvulus and is to be used cautiously in areas where Loa loa is prevalent because of severe adverse events observed with coinfected patients. METHODOLOGY/PRINCIPAL FINDINGS: This study investigated the anti-filarial activities of two Cameroonian medicinal plants, Lantana camara and Tamarindus indica locally used to treat onchocerciasis...
June 2018: PLoS Neglected Tropical Diseases
Rui Cai, Li Wan, Panpan Lyu, Lijuan Wang, Qiuyue Luo, Tingting Song, Qian Ding, Yaling Li, Deyi Yao, Shaoquan Xiong
OBJECTIVE: To construct an eukaryotic expression plasmid for AY358935 gene and explore its function. METHODS: cDNA of the AY358935 gene was amplified by reverse transcription-PCR and cloned into pGEM-Teasy. The pGEM-T-AY was validated by sequencing and served as a template for the construction of eukaryotic expression plasmid. The pcDNA3.1-AY recombinant was validated by double enzyme digestion and used for transient transfection of M14 cells. Expression of the AY358935 protein and proliferation of the M14 cells were determined respectively by Western blotting and 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) colorimetry...
June 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
Yue Wang, Meiling Tan, Haiping Ouyang, Linhong Deng
Ozone (O3 ) as a major air pollutant is widely recognized for causing pathological changes of the airway system. However, it is not clear whether O3 exposure of bronchial epithelial cells (BECs) influences the proliferation and collagen synthesis of submucosal fibroblasts and contributes to the pathogenesis of airway remodeling in diseases, including asthma. In the present study, a co-culture method was applied to culture human lung fibroblasts (HLFs) with human bronchial epithelial cells (HBECs) that were pre-stimulated with O3 ...
June 2018: Experimental and Therapeutic Medicine
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