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https://www.readbyqxmd.com/read/30515768/the-combination-of-metformin-and-2-deoxyglucose-significantly-inhibits-cyst-formation-in-miniature-pigs-with-polycystic-kidney-disease
#1
Xiaoying Lian, Xiaoyuan Wu, Zhongxin Li, Yingjie Zhang, Kangkang Song, Guangyan Cai, Qinggang Li, Shupeng Lin, Xiangmei Chen, Xue-Yuan Bai
BACKGROUND AND PURPOSE: The pathogenic mechanism of autosomal dominant polycystic kidney disease (ADPKD) is unclear. Similar to tumor cells, polycystic kidney cells are primarily dependent on aerobic glycolysis for ATP production. Compared with rodents, miniature pigs are more similar to humans. This study is the first time to investigate the effects of the combination of metformin and 2DG in a chronic progressive ADPKD miniature pig model. EXPERIMENTAL APPROACH: A miniature pig ADPKD model was established by inducible deletion of the PKD1 gene...
December 4, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/30489069/dual-mode-au-nanoprobe-based-on-surface-enhancement-raman-scattering-and-colorimetry-for-sensitive-determination-of-telomerase-activity-both-in-cell-extracts-and-the-urine-of-patients
#2
Enduo Feng, Tingting Zheng, Yang Tian
Telomerase is a valuable biomarker, which is highly correlated to cancer disease. However, single-mode probe for telomerase detection cannot satisfy the challenge about detection of telomerase activity rapidly, simply with high selectivity, sensitivity and accuracy in preliminary diagnosis and point of care (POC) testing. a novel dual-mode Au dimer probe was developed for quantifying telomerase activity both in cell extracts and the urine of patients based on SERS and colorimetry. The established probe possessed of high selectivity, sensitivity and accuracy, which was approved as a reliable, intuitional and convenient approach for detecting telomerase activity...
November 29, 2018: ACS Sensors
https://www.readbyqxmd.com/read/30486539/-protective-effects-of-sedum-sarmentosum-under-different-soil-moisture-in-ccl%C3%A2-induced-damage-in-hepg2-cells
#3
Jin-Feng Yang, Qiao-Sheng Guo, Zai-Biao Zhu, Wen-Xia Zhang, Neng-Jun Huang
At present, there were few studies about the effects of cultivation measures on the quality and pharmacological activity of medicinal plants. To explore the hepetoprotective activity of Sedum sarmentosum aqueous extracts after different water treatments, S. sarmentosum were planted under five water treatments for 60 days, namely 15%-20% FC (field capacity, S1), 35%-40% FC (S2), 55%-60% FC (S3), 75%-80% FC(S4), and 95%-100% FC (S5) and CCl₄ drug-induced liver injury model in vitro was used. Cell viability, cell cycle, and cell apoptosis were individually detected by MTT, PI single staining, and Annexin-V FITC/PI double staining assays...
October 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/30477629/-interactive-regulatory-effect-of-histone-h3k9ac-acetylation-and-histone-h3k9me3-methylation-on-cardiomyogenesis-in-mice
#4
Shuo Li, Xiao-Mei Luo, Bo-Hui Peng, Chang-Jian Yang, Chang Peng
OBJECTIVE: To study the interactive regulatory effect of histone acetylation and methylation on cardiomyogenesis, and to provide a theoretical basis for the prevention and treatment of congenital heart disease. METHODS: A total of 24 Kunming mice were randomly divided into embryo day 14.5 (ED 14.5) group, embryo day 16.5 (ED 16.5) group, postnatal day 0.5 (PND 0.5) group, and postnatal day 7 (PND 7) group, with 6 mice in each group, and the heart tissue of fetal and neonatal mice was collected...
November 2018: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/30468332/study-on-establishing-normal-ranges-of-chosen-biochemical-parameters-of-haemolymph-of-cornu-aspersum-maxima-and-cepaea-nemoralis-gastropods
#5
J Ziętek, L Guz, S Winiarczyk, K Szkucik, M Ziomek, M Wysokowski, J Madany, Ł Adaszek
The aim of the study was to establish normal ranges for chosen biochemical parameters of haemolymph of snails (Gasropoda: Mollusca), in the light of the use of these animals as experi- mental models in various types of studies. The study was conducted on 100 specimens of Cornu aspersum maxima (CAM) and 100 specimens of Cepaea nemoralis (CN). The haemolymph col- lected from the animals was analysed using colorimetry to assay aspartate transaminase (AST) activity, alanine transaminase (ALT) activity, amylase activity and the concentrations of urea and triglycerides...
September 2018: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/30463653/-alpha-lipoic-acid-inhibits-the-expression-of-%C3%AE-synuclein-in-pc12-cells-induced-by-6-hydroxydopamine-and-its-mechanism
#6
Shengyan Tai, Suzhen Zhai, Xiangming Zhang, Ling Jiao, Chunlin Zhang
Objective To investigate the effects of α-lipoic acid (α-LA) on the expression of α-synuclein(α-syn)in cellular models of Parkinson's disease (PD) induced by 6-hydroxydopamine (6-OHDA) and its mechanism. Methods PC12 cells were induced by 6-OHDA to establish the cellular models of PD, which was treated with α-LA. Cell viability was detected using MTT assay. The contents of intracellular iron and malondialdehyde (MDA) were detected by colorimetry.The level of intracellular reactive oxygen species (ROS) was detected by 2, 7-dichlorodihydrofluorescein diacetate (DCFH-DA) staining...
September 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/30455548/relationship-between-25-oh-d-levels-and-athletic-performance-in-elite-polish-judoists
#7
Anna Książek, Wioletta Dziubek, Jadwiga Pietraszewska, Małgorzata Słowińska-Lisowska
There is a growing body of evidence for a role of vitamin D in muscle function and for its influence on athletic performance, injury profile and recovery in well-trained athletes. The aim of our study was to assess the relationship between 25(OH)D levels and hand grip strength, lower limb isokinetic strength and muscle power in elite judoists. We enrolled 25 Polish elite judoists. The mean age was 21.9±9.8 years, the mean height was 179.2±6.6 cm, the mean body mass was 79.1±8.7 kg, and the mean career duration was 11...
June 2018: Biology of Sport
https://www.readbyqxmd.com/read/30430906/exposure-assessment-of-operators-to-pesticides-in-kongou-a-sub-watershed-of-niger-river-valley
#8
Karimoun M Illyassou, Rabani Adamou, Bruno Schiffers
The use of pesticides in horticultural sector in Niger has become an integral part of modern agriculture. Nevertheless, their inappropriate use can generate negative health effects to operators. A study was carried out among Kongou farmers in order to assess their potential dermal exposure (PDE). The UK-POEM model was used to quantify the PDE during mixing/loading and application according to the local practices. In order to determine which parts of the operator body are subject to most contamination during spraying and to validate the theoretical model used, a patch method was used with a tartrazine dye...
November 15, 2018: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/30384523/-different-arousal-modes-of-hibernating-whitmania-pigra
#9
Shi-Meng Yan, Qiao-Sheng Guo, Hong-Zhuan Shi, Bin Wu, Guo-Wei Shi, Man-Jun Wu, Shuai Wang, Xin Lu, Yi-Yun Liu
To study the suitable arousal modes of hibernating Whitmania pigra, the biological characteristics, activity of amylase, lipase, and protease as well as morphologic structure of digestive tract were investigated by direct observational method and 3, 5-dinitrosalicylic acid colorimetry, p-nitrophenyl palmitate ester(p-NPP)colorimetry, folin-phenol and histological methods. The results revealed that Wh. pigra activity was increased with increases of the water temperature and prolonging treating duration. Except for the feeding groups of direct arousal mode at 24 h and 48 h, none of the other Wh...
September 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/30382404/a-bimodal-sers-and-colorimetric-aptasensor-for-the-detection-of-pseudomonas-aeruginosa
#10
Zhengzong Wu, Deyun He, Bo Cui, Zhengyu Jin
An aptamer based assay is described for the determination of Pseudomonas aeruginosa (P. aeruginosa). It is bimodal in that both surface enhanced Raman spectroscopy (SERS) and colorimetry are applied for quantitation. The aptamer against P. aeruginosa and its corresponding complementary DNA fragment (cDNA) were conjugated to two kinds of differently sized gold nanoparticles (AuNPs). The 30 nm AuNPs carrying the aptamer are used as color signal probes, while the cDNA-15 nm AuNP conjugates serve as SERS signalling probes...
October 31, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/30351335/rapid-detection-of-multiple-respiratory-viruses-based-on-microfluidic-isothermal-amplification-and-a-real-time-colorimetric-method
#11
Ruili Wang, Rongtao Zhao, Yang Li, Wen Kong, Xudong Guo, Yi Yang, Feng Wu, Wanying Liu, Hongbin Song, Rongzhang Hao
Respiratory viruses are major threats causing development of acute respiratory tract infections, which are common causes of illness and death throughout the world. Here, an integrated microsystem based on real-time colorimetry was developed for diagnosing multiple respiratory viruses. The microsystem employed magnetic beads for nucleic acid extraction and an eight-channel microfluidic array chip integrated with a loop-mediated isothermal amplification system for point-of-care screening of respiratory viruses...
October 23, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/30339643/can-applications-designed-to-evaluate-visual-function-be-used-in-different-ipads
#12
Dolores de Fez, María José Luque, Mari Carmen García-Domene, Maria Teresa Caballero, Vicente J Camps
SIGNIFICANCE: Apple devices could be suitable for vision tests, provided that the test has been correctly adapted to the device, after considering the spatial and colorimetric characterization of the screen. PURPOSE: The majority of vision applications has not been developed by vision or colorimetry experts and suffers from conceptual and design errors that may lead average users to an erroneous assessment of their visual capabilities. The reliability of vision tests depends on the accurate generation of the necessary visual stimuli in a particular device...
November 2018: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/30325459/does-lipidomic-serum-analysis-support-the-assessment-of-digestive-efficiency-in-chickens
#13
Stéphane Beauclercq, Antoine Lefèvre, Lydie Nadal-Desbarats, Karine Germain, Christophe Praud, Patrick Emond, Elisabeth Le Bihan-Duval, Sandrine Mignon-Grasteau
The increasing cost of conventional feedstuffs used in poultry diets has bolstered interest in genetic selection for digestive efficiency (DE) to improve the adaptation of the birds to various alternative feedstuffs. However, DE measurement through AMEn is time-consuming and constraining. To simplify selection for DE, the potential of serum composition to predict AMEn was evaluated based on 40 birds from two broiler lines (D+ and D-) divergently selected on the fecal AMEn of a difficult-to-digest wheat-based diet...
October 16, 2018: Poultry Science
https://www.readbyqxmd.com/read/30322788/cooking-legumes-a-way-for-their-inclusion-in-the-renal-patient-diet
#14
Montserrat Martínez-Pineda, Cristina Yagüe-Ruiz, Alberto Caverni-Muñoz, Antonio Vercet-Tormo
OBJECTIVE: It has been observed that the consumption of legumes within a varied and Mediterranean diet has beneficial effects in prevention and control of many diseases, including chronic kidney disease (CKD). Recently, legumes have also been considered a good source of protein for CKD patients. However, despite their benefits, guidelines still recommend a limit to their consumption by these patients because of legumes' high potassium and phosphorus content, which are minerals whose intake must be controlled...
October 12, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/30318543/a-novel-paper-based-colorimetry-device-for-the-determination-of-the-albumin-to-creatinine-ratio
#15
Sudkate Chaiyo, Kurt Kalcher, Amara Apilux, Orawon Chailapakul, Weena Siangproh
A novel paper-based analytical device (PAD) was fabricated and developed for the simple and rapid determination of the albumin to creatinine ratio (ACR) in urine samples. The detection was based on a colorimetric reaction using bromocresol green (BG) in a phosphate buffer (PB) at pH 4 for the determination of albumin (AL) and creatinine (CR). BG changes color from greenish-yellow to bluish-green in the presence of AL and/or CR. Picric acid (PA) in 0.25 M NaOH was used to detect CR, and PA changes color from yellow to orange...
November 5, 2018: Analyst
https://www.readbyqxmd.com/read/30314259/potential-of-grape-wastes-as-a-natural-source-of-bioactive-compounds
#16
Guo-Yi Tang, Cai-Ning Zhao, Qing Liu, Xiao-Ling Feng, Xiao-Yu Xu, Shi-Yu Cao, Xiao Meng, Sha Li, Ren-You Gan, Hua-Bin Li
Grapes are widely used in the wine and juice industries, which can lead to massive amounts of waste, mostly grape peels and seeds. The antioxidant capacities, total phenolic and flavonoid contents and phenolic profiles of peels and seeds from 30 grape varieties were systemically assessed. The antioxidant activities of fat-soluble, water-soluble and insoluble-bound fractions of grape peels and seeds were evaluated using ferric-reducing antioxidant power and Trolox equivalent antioxidant capacity assays, and their total phenolic contents and total flavonoid contents were determined by the Folin-Ciocalteu method and AlCl₃ colorimetry, respectively...
October 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30309551/impact-of-a-post-fermentative-maceration-with-overripe-seeds-on-the-color-stability-of-red-wines
#17
Francisco J Rivero, M José Jara-Palacios, Belén Gordillo, Francisco J Heredia, M Lourdes González-Miret
With the purpose of modulating the copigmentation equilibria of red wines, an environmentally sustainable process was performed based on post-fermentative addition of overripe seeds (OS). Simple (SW) and double (DW) addition were performed to produce different enrichment of phenolics from seeds, hence different copigmentation/polymerization ratios. The determination of the phenolic composition showed different global increases in OS-macerates wines (catechin, epicatechin, gallic acid and procyanidins B1 and B2)...
January 30, 2019: Food Chemistry
https://www.readbyqxmd.com/read/30302673/analysis-of-multifloral-bee-pollen-pellets-by-advanced-digital-imaging-applied-to-functional-food-ingredients
#18
Claudia Y Salazar-González, Francisco J Rodríguez-Pulido, Anass Terrab, Consuelo Díaz-Moreno, Carlos A Fuenmayor, Francisco J Heredia
Bee pollen is a hive product, resulting from floral pollen agglutination by worker bees and it is characterized by its excellent bioactive and nutritional composition. Currently, research is focused on bee pollen applications on food industry, because this product has been considered an excellent source of compounds for human nutrition. It is also important in some industries, where color and particle size are important characteristics for production. Due to the granular nature of bee pollen, conventional colorimetry does not allow describing color correctly; thus, digital image analysis is a better alternative...
December 2018: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/30292149/portable-and-selective-colorimetric-film-and-digital-image-colorimetry-for-detection-of-iron
#19
Aree Choodum, Wilasinee Sriprom, Worawit Wongniramaikul
Iron is an important trace element in environmental and biological systems, the development of simple and selective methods for the determination of iron is important. In this work, completely biodegradable tapioca starch was introduced as the substrate to entrap standard chromogenic probes (1,10-phenanthroline) for fabrication of a novel colorimetric sensor for ferrous. A clear plasticized thin film from tapioca starch was fabricated inside a small plastic tube as a portable test kit. A red complex was obtained by exposing the film to a ferrous solution, while no color changes were obtained with various other ions, indicating excellent selectivity...
February 5, 2019: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/30289315/regulation-of-free-fatty-acids-by-sitagliptin-monotherapy-in-drug-na%C3%A3-ve-subjects-with-type-2-diabetes
#20
Eiji Kutoh, Asuka Wada, Jyunka Hayashi
OBJECTIVES: The aim of this study is to investigate the effects of sitagliptin on the regulation of FFA and other metabolic parameters in drug-naïve subjects with T2DM. SUBJECTS AND METHODS: This is a prospective, non-randomized, observational study. Drug naïve subjects with T2DM received 25-50 mg/day sitagliptin monotherapy (n=64). At 3 months, FFA and other metabolic parameters were compared with those at baseline. FFA was measured by colorimetry with enzymatic reactions...
October 5, 2018: Endocrine Practice
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