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Coiled Coil

Malay Sharma, Piyush Somani, Tagore Sunkara, Ritesh Prajapati, Rahul Talele
No abstract text is available yet for this article.
December 11, 2018: Endoscopy
Kota Kasahara, Shintaro Minami, Yasunori Aizawa
The principle of three-dimensional protein structure formation is a long-standing conundrum in structural biology. A globular domain of a soluble protein is formed by a network of atomic contacts among amino acid residues, but regions without intramolecular non-local contacts are often observed in the protein structure, especially in loop, linker, and peripheral segments with secondary structures. Although these regions can play key roles for protein function as interfaces for intermolecular interactions, their nature remains unclear...
2018: PloS One
Gokhan Ariturk, Yusuf Ziya Ider
Non-quadrature radio frequency (RF) excitation has been widely studied in the fields of RF shimming, local SAR estimation, and MR-EPT with the use of multi-channel transceiver arrays. These studies generally require the retrieval of the complex transmit field B<sub>1</sub><sup>+</sup>, which can be accomplished by acquiring its magnitude and phase in different steps. Magnitude of the transmit field is acquired with the conventional methods which give accurate results for both quadrature and non-quadrature excitations...
December 11, 2018: Physics in Medicine and Biology
Imtiyaz Ahmad Bhat, Bibhisan Roy, Partha Hazra, Kabir-Ud-Din
Herein, we have explored the conformational alterations of hemoglobin (Hb) in presence of a cleavable gemini surfactant (C16-C4O2-C16). The concerned surfactant was found to induce significant structural perturbations in Hb. UV-vis spectroscopy, steady-state/time-resolved fluorescence, and other utilized techniques have authenticated the complexation of Hb with the gemini surfactant. CD has demonstrated the alterations in secondary structural elements (α-helicity, β-sheet, β-turn, and random coil) of Hb upon C16-C4O2-C16 addition...
December 3, 2018: Journal of Colloid and Interface Science
Orazio Valsecchi, Angelina Vassileva, Francesco Martella, Alberto Francesco Cereda
BACKGROUND: Trans-catheter closure of coronary artery fistulae (CAF) has become a successful alternative to cardiac surgery with excellent results and acceptable low rate of complications. Different techniques and devices are available to treat the extreme anatomical variability of CAF. AIM: We retrospectively describe our case series of five patients trans-radially treated using an Amplatzer vascular plug IV (AVP-IV) with telescoping catheter technique. RESULTS: Trans-radial closure of CAF using the "Child in Mother" technique with dedicated catheters to deliver the vascular plugs AVP-IV was successfully performed in all patients, in one case for a complex CAF, an hybrid step approach using coils and plugs was needed...
December 7, 2018: Catheterization and Cardiovascular Interventions
Jianqun Zheng, Hao Liu, Lei Zhu, Yawen Chen, Huijie Zhao, Wei Zhang, Fan Li, Lele Xie, Xiumin Yan, Xueliang Zhu
Cilia are cellular protrusions containing nine microtubule (MT) doublets and function to propel cell movement or extracellular liquid flow through beating or sense environmental stimuli through signal transductions. Cilia require the central pair (CP) apparatus, consisting of two CP MTs covered with projections of CP proteins, for planar strokes. How the CP MTs of such '9 + 2' cilia are constructed, however, remains unknown. Here we identify Spef1, an evolutionarily conserved microtubule-bundling protein, as a core CP MT regulator in mammalian cilia...
February 23, 2018: Journal of Molecular Cell Biology
Serge Marbacher, Alexander Spiessberger, Michael Diepers, Luca Remonda, Javier Fandino
Microsurgical clip ligation is considered a definitive treatment for intracranial aneurysms (IAs), resulting in low rates of local recurrence that range from 0.2 to 0.5% and a latency period that averages about a decade. Our case report describes an early asymptomatic recurrence (i.e., without sentinel headache or seizure) less than 1 year after this 20-year-old woman underwent clip ligation of a ruptured anterior communicating artery (AComA) aneurysm. At recurrence, the patient underwent coiling of the regrowth; follow-up imaging at 6 and 18 months demonstrated complete IA occlusion...
October 2018: Journal of Neurological Surgery Reports
Miro Janco, Till Böcking, Stanley He, Adelle C F Coster
Skeletal muscle tropomyosin (Tpm1.1) is an elongated, rod-shaped, alpha-helical coiled-coil protein that forms continuous head-to-tail polymers along both sides of the actin filament. In this study we use single molecule fluorescence TIRF microscopy combined with a microfluidic device and fluorescently labelled proteins to measure Tpm1.1 association to and dissociation from single actin filaments. Our experimental setup allows us to clearly resolve Tpm1.1 interactions on both sides of the filaments. Here we provide a semi-automated method for the extraction and quantification of kymograph data for individual actin filaments bound at different Tpm1...
2018: PloS One
Tina Fink, Jan Lonzarić, Arne Praznik, Tjaša Plaper, Estera Merljak, Katja Leben, Nina Jerala, Tina Lebar, Žiga Strmšek, Fabio Lapenta, Mojca Benčina, Roman Jerala
Cellular signal transduction is predominantly based on protein interactions and their post-translational modifications, which enable a fast response to input signals. Owing to difficulties in designing new unique protein-protein interactions, designed cellular logic has focused on transcriptional regulation; however, that process has a substantially slower response, because it requires transcription and translation. Here, we present de novo design of modular, scalable signaling pathways based on proteolysis and designed coiled coils (CC) and implemented in mammalian cells...
December 10, 2018: Nature Chemical Biology
Wei-Wei Pan, Toshiro Moroishi, Ja Hyun Koo, Kun-Liang Guan
The Hippo pathway controls organ size and tissue homeostasis, and its dysregulation often contributes to tumorigenesis. Extensive studies have shown that the Hippo pathway inhibits cell proliferation, and survival in a cell-autonomous manner. We examined the function of the Hippo pathway kinases LATS1/2 (large tumor suppressor 1 and 2) in cancer cells. As expected, loss of LATS1/2 promotes cancer cell growth in most cell lines. Surprisingly, however, LATS1/2 deletion inhibits the growth of murine MC38 colon cancer cells, especially under detachment conditions...
December 10, 2018: Oncogene
Hidehisa Nishi, Akira Ishii, Tetsu Satow, Koji Iihara, Nobuyuki Sakai
Although the current standard treatment for unruptured aneurysms comprises surgical clipping or endovascular coiling, these techniques are not suitable for some cases, such as large, giant, and fusiform aneurysms. Endovascular parent artery occlusion (PAO), which includes internal trapping and proximal occlusion, is a well-established alternative treatment for such cases. Here, we retrospectively reviewed PAO cases from the Japanese Registry of Neuroendovascular Therapy 3, a nation-wide survey of all neuroendovascular therapy cases between 2010 and 2014...
December 7, 2018: Neurologia Medico-chirurgica
Jawdat Al-Bassam, Stanley Nithianantham
No abstract text is available yet for this article.
December 7, 2018: Proceedings of the National Academy of Sciences of the United States of America
Chengyuan Mao, Herui Wang, Haiyang Luo, Shuyu Zhang, Huisha Xu, Shuo Zhang, Jared Rosenblum, Zhilei Wang, Qi Zhang, Mibo Tang, Matthew J Shepard, Xiang Wang, Yaohe Wang, Zhengping Zhuang, Changhe Shi, Yuming Xu
Previously we identified the p.Thr61Ile mutation in coiled-coil-helix-coiled-coil-helix domain containing 2 (CHCHD2) in a Chinese family with autosomal dominant Parkinson's disease. But the mechanism is still unclear. In this study, we explored the effects of CHCHD2 p.Thr61Ile mutation in cells and its association with coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10). We found that overexpression of Parkinson's disease-associated T61I mutant CHCHD2 did not produce mitochondrial dysfunction...
October 23, 2018: Neurobiology of Aging
Zhenzhen Zhou, Ke Wei, Jianshe Zhang
In this study, we identified and characterized a tripartite motif containing 25 (TRIM25) gene homologue, LcTRIM25, from large yellow croaker (Larimichthys crocea). Two isoforms of LcTRIM25, which were generated via alternative splicing, were identified via a molecular analysis of cDNA clones. The long isoform of LcTRIM25 (termed as LcTRIM25-L) contained the full open reading frame of the gene, encoded a protein of 698 amino acid residues, and possessed 11 exons. The short isoform of LcTRIM25 (termed as LcTRIM25-S) contained 9 exons and encoded a protein of 665 amino acid residues...
December 7, 2018: Fish & Shellfish Immunology
Alexander V Fonin, Olga V Stepanenko, Asiia K Sitdikova, Iuliia A Antifeeva, Elena I Kostyleva, Alexander M Polyanichko, Maksim M Karasev, Sergey A Silonov, Olga I Povarova, Irina M Kuznetsova, Vladimir N Uversky, Konstantin К Turoverov
pH-induced structural changes of the synthetic homopolypeptides poly-E, poly-K, poly-R, and intrinsically disordered proteins (IDPs) prothymosin α (ProTα) and linker histone H1, in concentrated PEG solutions simulating macromolecular crowding conditions within the membrane-less organelles, were characterized. The conformational transitions of the studied poly-amino acids in the concentrated PEG solutions depend on the polymerization degree of these homopolypeptides, the size of their side chains, the charge distribution of the side chains, and the crowding agent concentration...
December 5, 2018: International Journal of Biological Macromolecules
Daniel L Drew, Tanbir Ahammad, Rachel A Serafin, Brandon J Butcher, Katherine R Clowes, Zachary Drake, Indra D Sahu, Robert M McCarrick, Gary A Lorigan
The mechanism for the lysis pathway of double-stranded DNA bacteriophages involves a small hole-forming class of membrane proteins, the holins. This study focuses on a poorly characterized class of holins, the pinholin, of which the S21 protein of phage ϕ21 is the prototype. Here we report the first in vitro synthesis of the wildtype form of the S21 pinholin, S21 68, and negative-dominant mutant form, S21 IRS, both prepared using solid phase peptide synthesis and studied using biophysical techniques. Both forms of the pinholin were labeled with a nitroxide spin label and successfully incorporated into both bicelles and multilamellar vesicles which are membrane mimetic systems...
December 4, 2018: Analytical Biochemistry
Li Xiaoli, Zou Wujun, Liu Jing
Acute lung injury (ALI) and its more serious form, respiratory distress syndrome (ARDS), are considered as an acute and severe inflammatory process existing in lungs, and still remain high mortality rates. Tripartite motif 8 (TRIM8) contains an N-terminal RING finger, which is followed by two B-boxes and a coiled-coil domain, belonging to the TRIM/RBCC family and playing significant role in meditating inflammation, oxidative stress and apoptosis. In the study, we investigated the role of TRIM8 in ALI induced by lipopolysaccharide (LPS) and the underlying molecular mechanisms...
December 4, 2018: Biochemical and Biophysical Research Communications
Jaehyun Yim, Dongho Hyun, Sung Ki Cho, Kwang Bo Park, Hong Suk Park, Sung Wook Shin, Dong Wook Choi, Seonwoo Kim, Sung-Young Baek, Sang Yub Lee
PURPOSE: To retrospectively investigate the impact of hyperbilirubinemia on future liver remnant (FLR) volume after percutaneous transhepatic portal vein embolization (PVE) and incidence of post-hepatectomy liver failure in primary biliary malignancy. MATERIALS AND METHODS: Eighty-seven patients (62 men, overall mean age 66.9 y) who underwent PVE, using Gelfoam and coils before major hepatectomy between January 2004 and June 2016, were included in this study and divided into a hyperbilirubinemia (serum total bilirubin level at PVE 5...
December 6, 2018: Journal of Vascular and Interventional Radiology: JVIR
Yilmaz Onal, Bulent Acunas, Cesur Samanci, Murat Ugurlucan, Muzaffer Reha Umutlu, Didem Melis Oztas, Ufuk Alpagut
PURPOSE: To determine technical feasibility and results of stent-assisted coiling of visceral artery aneurysms (VAAs) with self-expandable LEO PLUS neurointerventional stent. MATERIALS AND METHODS: In this retrospective study, 11 consecutive patients (mean age 61.9 y ± 8.9; range, 45-76 y) with VAAs (splenic, n = 3; common hepatic, n = 2; renal, n = 5; celiac, n = 1) treated with neurointerventional stents were included. MR angiography was evaluated for aneurysmal occlusion and patency of the parent arteries...
December 6, 2018: Journal of Vascular and Interventional Radiology: JVIR
Domniţa-Valeria Rusnac, Hsiu-Chuan Lin, Daniele Canzani, Karena X Tien, Thomas R Hinds, Ashley F Tsue, Matthew F Bush, Hsueh-Chi S Yen, Ning Zheng
Aberrant proteins can be deleterious to cells and are cleared by the ubiquitin-proteasome system. A group of C-end degrons that are recognized by specific cullin-RING ubiquitin E3 ligases (CRLs) has recently been identified in some of these abnormal polypeptides. Here, we report three crystal structures of a CRL2 substrate receptor, KLHDC2, in complex with the diglycine-ending C-end degrons of two early-terminated selenoproteins and the N-terminal proteolytic fragment of USP1. The E3 recognizes the degron peptides in a similarly coiled conformation and cradles their C-terminal diglycine with a deep surface pocket...
December 6, 2018: Molecular Cell
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