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Daniel J Spade, Edward Dere, Susan J Hall, Christoph Schorl, Richard N Freiman, Kim Boekelheide
Exposure to excess retinoic acid disrupts the development of the mammalian testicular seminiferous cord. However, the molecular events surrounding retinoic acid-driven loss of cord structure have not previously been examined. To investigate the mechanisms associated with this adverse developmental effect, fetal rat testes were isolated on gestational day 15, after testis determination and the initiation of cord development, and cultured in media containing all-trans retinoic acid (ATRA; 10-8 to 10-6 M) or vehicle for 3 days...
October 17, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Saad Wan Zuhainis, Mohammad Navaderi, Rosfarizan Mohamad
Background: In recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression. Objectives: The aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties...
2018: International Journal of Nanomedicine
Priyanka, Vidisha Tripathi, Rajiva Raman
Foxl2,Rspo1, and Aromatase are genes important in the ovary developmental pathway in mammals and birds. Here, we show their presence in the lizard, Calotes versicolor, which is known to lack a chromosomal as well as a temperature-dependent mode of sex determination and has an indeterminate, bipotential gonad throughout embryonic development. The expression of the 3 genes, as well as that of CvSox9 and Wnt4 - the known testis and ovary pathway genes - was studied by RT-PCR and whole tissue RNA in situ hybridization (WRISH) on the developing mesonephros gonadal complex (MGC)...
September 15, 2018: Sexual Development: Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation
Niko Välimäki, Heli Kuisma, Annukka Pasanen, Oskari Heikinheimo, Jari Sjöberg, Ralf Bützow, Nanna Sarvilinna, Hanna-Riikka Heinonen, Jaana Tolvanen, Simona Bramante, Tomas Tanskanen, Juha Auvinen, Terhi Piltonen, Amjad Alkodsi, Rainer Lehtonen, Eevi Kaasinen, Kimmo Palin, Lauri A Aaltonen
Uterine leiomyomas (ULs) are benign tumors that are a major burden to women's health. A genome-wide association study on 15,453 UL cases and 392,628 controls was performed, followed by replication of the genomic risk in six cohorts. Effects of the risk alleles were evaluated in view of molecular and clinical characteristics. 22 loci displayed a genome-wide significant association. The likely predisposition genes could be grouped to two biological processes. Genes involved in genome stability were represented by TERT, TERC, OBFC1 - highlighting the role of telomere maintenance - TP53 and ATM ...
September 18, 2018: ELife
Noriko Katoh, Keiji Kuroda, Junko Tomikawa, Hiroko Ogata-Kawata, Rie Ozaki, Asako Ochiai, Mari Kitade, Satoru Takeda, Kazuhiko Nakabayashi, Kenichiro Hata
AIM: Decidualization is essential for embryo implantation and placental development. We aimed to obtain transcriptome and epigenome profiles for primary endometrial stromal cells (ESCs) and in vitro decidualized cells. MATERIALS & METHODS: ESCs isolated from human endometrial tissues remained untreated (D0), or decidualized for 4 days (D4) and 8 days (D8) in the presence of 8-bromo-cAMP and progesterone. RESULTS: Among the epigenetic modifications examined (DNA methylation, H3K27ac, H3K9me3 and H3K27me3), the H3K27ac patterns changed most dramatically, with a moderate correlation with gene expression changes, upon decidualization...
September 2018: Epigenomics
Thorunn Rafnar, Bjarni Gunnarsson, Olafur A Stefansson, Patrick Sulem, Andres Ingason, Michael L Frigge, Lilja Stefansdottir, Jon K Sigurdsson, Vinicius Tragante, Valgerdur Steinthorsdottir, Unnur Styrkarsdottir, Simon N Stacey, Julius Gudmundsson, Gudny A Arnadottir, Asmundur Oddsson, Florian Zink, Gisli Halldorsson, Gardar Sveinbjornsson, Ragnar P Kristjansson, Olafur B Davidsson, Anna Salvarsdottir, Asgeir Thoroddsen, Elisabet A Helgadottir, Katrin Kristjansdottir, Orri Ingthorsson, Valur Gudmundsson, Reynir T Geirsson, Ragnheidur Arnadottir, Daniel F Gudbjartsson, Gisli Masson, Folkert W Asselbergs, Jon G Jonasson, Karl Olafsson, Unnur Thorsteinsdottir, Bjarni V Halldorsson, Gudmar Thorleifsson, Kari Stefansson
Uterine leiomyomas are common benign tumors of the myometrium. We performed a meta-analysis of two genome-wide association studies of leiomyoma in European women (16,595 cases and 523,330 controls), uncovering 21 variants at 16 loci that associate with the disease. Five variants were previously reported to confer risk of various malignant or benign tumors (rs78378222 in TP53, rs10069690 in TERT, rs1800057 and rs1801516 in ATM, and rs7907606 at OBFC1) and four signals are located at established risk loci for hormone-related traits (endometriosis and breast cancer) at 1q36...
September 7, 2018: Nature Communications
Swamy K Tripurani, Yan Wang, Ying-Xin Fan, Massod Rahimi, Lily Wong, Min-Hyung Lee, Matthew F Starost, Jeffrey S Rubin, Gibbes R Johnson
Mice that lack EGFR fail to develop a hair coat, but the mechanism responsible for this deficit is not completely understood. Here, we show that EGFR plays a critical role to attenuate Wnt/β-catenin signaling during postnatal hair follicle development. Genetic ablation of EGFR in mice resulted in increased mitotic activity in matrix cells, apoptosis in hair follicles and impaired differentiation of epithelial lineages that form hair. EGFR is activated in wild type hair follicle stem cells marked with SOX9 or NFATc1, and is essential to restrain proliferation, support stem cell numbers and their quiescence...
September 6, 2018: Molecular Biology of the Cell
Piyush Chaudhary, Sudha Saryu Malhotra, Gosipatala Sunil Babu, Ranbir Chander Sobti, Satish Kumar Gupta
Inadequate migration and invasion of the trophoblast cells during embryo implantation is one of the reasons for pregnancy-related complications such as intrauterine growth restriction and preeclampsia. In the present study, relevance of WNT ligands and integrins associated with hepatocyte growth factor (HGF)-mediated migration of HTR-8/SVneo trophoblastic cells has been investigated. Treatment of HTR-8/SVneo cells with HGF led to a dose-dependent increase in their migration. RT-PCR studies revealed a significant increase in the transcripts of WNT4, WNT11, ITGA2, and ITGAV, which was further confirmed at protein level by Western blotting...
August 22, 2018: Molecular and Cellular Biochemistry
Hui Zhang, Khalid Mehmood, Xiong Jiang, Wangyuan Yao, Mujahid Iqbal, Muhammad Waqas, Mujeeb Ur Rehman, Aoyun Li, Yaoqin Shen, Jiakui Li
Tetramethyl thiuram disulfide (thiram) is one of the important pesticides, which is extensively used in agriculture, but if it is combined with the cell membrane, then it causes membrane damage, bone morphogenic inactivation, and inhibited angiogenesis. Thiram has been considered a common cause of tibial dyschondrolplasia (TD) in various avian species, because it becomes the part of feed due to environmental contamination and its overuse in agriculture as pesticides or fungicide. However, there is no systematic study on the changes of the correlation indexes with toxic effect of the thiram in chickens...
October 2018: Environmental Science and Pollution Research International
Guillaume St-Jean, Alexandre Boyer, Gustavo Zamberlam, Philippe Godin, Marilène Paquet, Derek Boerboom
Both Wnt4 and Wnt5a have well-established roles in the embryonic development of the female reproductive tract, as well as in implantation, decidualization and ovarian function in adult mice. Although these roles appear to overlap, whether Wnt5a and Wnt4 are functionally redundant in these tissues has not been determined. Here, we addressed this by concomitantly inactivating Wnt4 and Wnt5a in the Müllerian mesenchyme and in ovarian granulosa cells by crossing mice bearing floxed alleles to the Amhr2cre strain...
July 13, 2018: Biology of Reproduction
Maciej Osiński, Adrianna Mostowska, Przemyslaw Wirstlein, Ewa Wender-Ożegowska, Paweł Piotr Jagodziński, Małgorzata Szczepańska
OBJECTIVES: Genome-wide association studies in patients with endometriosis revealed ten significant single nucleo-tide polymorphisms (SNPs) in the Caucasian population, which include rs12700667 near NFE2L3, rs12037376 in WNT4, rs7521902 near WNT4, rs13394619 in GREB1, rs10859871 near VEZT, rs1537377 near CDKN2B-AS1, rs4141819 near ETAA1, rs7739264 near ID4, rs1519761 near RND3 and rs6542095 near IL1A. MATERIAL AND METHODS: We replicated ten polymorphisms among infertile women with endometriosis (n = 315) and healthy fertile women (n = 406) in the Polish Caucasian population...
2018: Ginekologia Polska
Yi-Xin He, Tian-Tian Diao, Shu-Min Song, Cen-Cen Wang, Yu Wang, Chun-Lan Zhou, Yi-Bing Bai, Shan-Shen Yu, Xuan Mi, Xin-Yu Yang, Qiu-Ju Wei, Bing Li
Wnt4 is a secreted growth factor associated with renal tubulogenesis. Our previous studies identified that renal and urinary Wnt4 are upregulated following ischemia-reperfusion injury in mice, but the roles of Wnt4 in other forms of acute kidney injury (AKI) remain unclear. Here, we investigated the changes in Wnt4 expression using a cisplatin-induced AKI model. We found that renal and urinary Wnt4 expression increased as early as 12 hours, peaked at day 4 following cisplatin-induced AKI and was closely correlated with histopathological alterations...
July 12, 2018: Scientific Reports
C L Liu, L Yan, K R Cai, K Sun, Y Qi, Y L Han, X D Zhang, X D Sun
To observe the effect of Soyisoflavones (SI) on the expression of Wnt/β-catenin signaling pathway elements, transforming growth factor-β (THGF-β) and its related proteins in the renal interstitia of diabetic nephropathic (DN) rats, 48 DN rats were randomly divided into 4 groups: DN model group (group DN), soybean isoflavone treatment group (group DA), DN model group + losartan treatment group (group DL), DN model group + soybean isoflavones combined with losartan treatment group (group SL). Each group comprised 12 rats...
May 2018: Journal of Biological Regulators and Homeostatic Agents
Vinay Shukla, Pooja Popli, Jyoti Bala Kaushal, Kanchan Gupta, Anila Dwivedi
Tubulin polymerization promoting protein 3 (TPPP3) is known to be expressed in the endometrium in a cyclic manner, its functional role in the physiology of implantation remains unknown. Here we demonstrate a novel function of TPPP3 during the window of implantation and in the establishment of pregnancy using a mouse model. The increased protein expression of TPPP3 and β-catenin during peri-implantation period i.e. D5 (receptive phase, 0800 h) was observed as compared to that on D1 (non-receptive phase, 0800 h)...
June 12, 2018: Biology of Reproduction
Celia L Gregson, Felicity Newell, Paul J Leo, Graeme R Clark, Lavinia Paternoster, Mhairi Marshall, Vincenzo Forgetta, John A Morris, Bing Ge, Xiao Bao, J H Duncan Bassett, Graham R Williams, Scott E Youlten, Peter I Croucher, George Davey Smith, David M Evans, John P Kemp, Matthew A Brown, Jon H Tobias, Emma L Duncan
BACKGROUND: Generalised high bone mass (HBM), associated with features of a mild skeletal dysplasia, has a prevalence of 0.18% in a UK DXA-scanned adult population. We hypothesized that the genetic component of extreme HBM includes contributions from common variants of small effect and rarer variants of large effect, both enriched in an extreme phenotype cohort. METHODS: We performed a genome-wide association study (GWAS) of adults with either extreme high or low BMD...
September 2018: Bone
Tatiana V Novoselova, Mashal Hussain, Peter J King, Leonardo Guasti, Louise A Metherell, Marika Charalambous, Adrian J L Clark, Li F Chan
Melanocortin 2 receptor accessory protein (MRAP) is a single transmembrane domain accessory protein and a critical component of the hypothamo-pituitary-adrenal axis. MRAP is highly expressed in the adrenal gland and is essential for adrenocorticotropin hormone (ACTH) receptor expression and function. Human loss-of-function mutations in MRAP cause familial glucocorticoid (GC) deficiency (FGD) type 2 (FGD2), whereby the adrenal gland fails to respond to ACTH and to produce cortisol. In this study, we generated Mrap-null mice to study the function of MRAP in vivo...
June 7, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Wan Zuhainis Saad, Mohammad Navaderi, Palanisamy Arulselvan, Rosfarizan Mohamad
Background: Molecular investigation of wound healing has allowed better understanding about interaction of genes and pathways involved in healing progression. Objectives: The aim of this study was to prepare magnetic/bacterial nanocellulose (Fe3 O4 /BNC) nanocomposite films as ecofriendly wound dressing in order to evaluate their physical, cytotoxicity and antimicrobial properties. The molecular study was carried out to evaluate expression of genes involved in healing of wounds after treatment with BNC/Fe3 O4 films...
2018: International Journal of Nanomedicine
Mariam Lofty Khaled, Yelena Bykhovskaya, Sarah E R Yablonski, Hanzhou Li, Michelle D Drewry, Inas F Aboobakar, Amy Estes, X Raymond Gao, W Daniel Stamer, Hongyan Xu, R Rand Allingham, Michael A Hauser, Yaron S Rabinowitz, Yutao Liu
Purpose: Keratoconus (KC) is the most common corneal ectasia. We aimed to determine the differential expression of coding and long noncoding RNAs (lncRNAs) in human corneas affected with KC. Methods: From the corneas of 10 KC patients and 8 non-KC healthy controls, 200 ng total RNA was used to prepare sequencing libraries with the SMARTer Stranded RNA-Seq kit after ribosomal RNA depletion, followed by paired-end 50-bp sequencing with Illumina Sequencer. Differential analysis was done using TopHat/Cufflinks with a gene file from Ensembl and a lncRNA file from NONCODE...
June 1, 2018: Investigative Ophthalmology & Visual Science
Razieh Taghizadeh Ghavamabadi, Zahra Taghipour, Mahsa Hassanipour, Marzieh Khademi, Mehdi Shariati
Objectives: Depression during pregnancy negatively affects fetal development. Fluoxetine as a selective serotonin reuptake inhibitor (SSRIs) is used for treatment of gestational depression. This study is trying to determine the effects of fluoxetine on the renal, heart and lung development. Materials and Methods: Fifteen pregnant rats were treated with fluoxetine at 7 mg/kg from days 0 to 21 of gestation. Immediately after born, heart and kidney samples were evaluated for genes expression and histological assessment...
April 2018: Iranian Journal of Basic Medical Sciences
Yu Chen, Hongshi Yu, Andrew J Pask, Asao Fujiyama, Yutaka Suzuki, Sumio Sugano, Geoff Shaw, Marilyn B Renfree
Environmental endocrine disruptors (EEDs) that affect androgen or estrogen activity may disrupt gene regulation during normal phallus development to cause hypospadias or a masculinized clitoris. We treated developing male tammar wallabies with estrogen and females with androgen from day 20-40 postpartum (pp) during the established androgen imprinting window. Administration of estrogen to males inhibited phallus elongation but had no effect on urethral closure and did not significantly depress testicular androgen synthesis...
May 14, 2018: Biology of Reproduction
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