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Luca Boeri, Paolo Capogrosso, Eugenio Ventimiglia, Filippo Pederzoli, Walter Cazzaniga, Francesco Chierigo, Edoardo Pozzi, Massimo Clementi, Paola Viganò, Emanuele Montanari, Francesco Montorsi, Andrea Salonia
STUDY QUESTION: Does the presence of human papillomavirus (HPV) in semen impact seminal parameters and sperm DNA quality in white European men seeking medical help for primary couple's infertility? SUMMARY ANSWER: HPV seminal infections involving high-risk (HR) genotypes are associated with impaired sperm progressive motility and sperm DNA fragmentation (SDF) values. WHAT IS KNOWN ALREADY: HPV is commonly present in semen samples. However, whether the presence of HPV in semen is actually associated with impaired sperm parameters and SDF values have yet to be elucidated...
December 4, 2018: Human Reproduction
Miaomei Yu, Yun Xu, Lili Pan, Yuehua Feng, Kaiming Luo, Qinfeng Mu, Guanghua Luo
BACKGROUND/AIMS: microRNAs (miRNAs) are known to act as oncogenes or tumor suppressors in diverse cancers. Although miR-10b is an oncogene implicated in many tumors, its role in cervical cancer (CC) remains largely unclear. Here, we investigated the function and underlying mechanisms of miR-10b in human CC. METHODS: Quantitative RT-PCR was used to measure miR-10b expression in CC and normal tissues, and its association with clinicopathologic features was analyzed...
November 30, 2018: Cellular Physiology and Biochemistry
Alice Bernard-Tessier, Emmanuelle Jeannot, Guenat David, Alice Debernardi, Marc Michel, Charlotte Proudhon, Anne Vincent-Salomon, Ivan Bieche, Jean-Yves Pierga, Bruno Buecher, Aurélia Meurisse, Eric François, Romain Cohen, Marine Jary, Véronique Vendrely, Emmanuelle Samalin, Farid El Hajbi, Nabil Baba-Hamed, Christophe Borg, Francois-Clement Bidard, Stefano Kim
PURPOSE: Human papillomavirus (HPV) is found in 90% of squamous cell carcinomas of the anal canal (SCCA). We investigated the clinical validity of HPV circulating tumor DNA (ctDNA) detection in patients enrolled in the Epitopes-HPV02 trial which demonstrated the efficacy of docetaxel, cisplatin and 5FU as first-line chemotherapy (CT) in advanced SCCA. EXPERIMENTAL DESIGN: According to the protocol, serum samples were collected before CT and on completion of CT. HPV16 ctDNA was quantified by ddPCR and correlated with prospectively registered patient characteristics and outcomes...
November 30, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Anna Bonhoure, Auguste Demenge, Camille Kostmann, Leticia San José, Eva De la Cal, Pilar Armisen, Yves Nominé, Gilles Travé
BACKGROUND: Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-association may still occur. For instance, HPV E6 oncoproteins, when produced as MBP-E6 fusions, are expressed as mixtures of biologically inactive oligomers and active monomers. While a protocol was previously developed to isolate MBP-E6 monomers for structural studies, it allows the purification of only one MBP-E6 construct at the time...
December 1, 2018: Microbial Cell Factories
M Arbyn, L Xu
Recently, the evidence on efficacy and safety of prophylactic HPV vaccines derived from randomized trials was published in the Cochrane database of Systematic reviews. A summary of this Cochrane review is presented below. Areas covered: Only trials involving mono-, bi-, and quadrivalent HPV vaccines were included. Trials evaluating the nonavalent vaccine were excluded since women in the control group received the quadrivalent vaccine. Main efficacy outcomes were: histologically confirmed cervical precancer lesions distinguishing those associated with vaccine HPV types and any cervical precancer...
November 29, 2018: Expert Review of Vaccines
Giorgio Bogani, Elena Tagliabue, Stefano Ferla, Fabio Martinelli, Antonino Ditto, Valentina Chiappa, Umberto Leone Roberti Maggiore, Francesca Taverna, Claudia Lombardo, Domenica Lorusso, Francesco Raspagliesi
The widespread introduction of screening methods allow to identify cervical dysplasia before having invasive cancer. The risk of developing cervical dysplasia persistence/recurrence following conization represent a major health issue. Although several studies tried to identify predictors for cervical dysplasia persistence/recurrence, no previous study has been conducted to develop a risk calculator. The current study aimed to identify predictors of cervical dysplasia persistence/recurrence among women undergoing primary conization...
November 26, 2018: European Journal of Cancer Prevention
Mayinuer Alifu, Yun-Hui Hu, Tao Dong, Ruo-Zheng Wang
Objective: This study aims to investigate the distribution of HLA-A genes and identify alleles related to cervical cancer. Materials and Methods: A total of 252 cervical cancer patients (56 Han ethnic and 196 Uyghur ethnic) and 213 controls (103 Han ethnic and 110 Uyghur ethnic) were recruited in this study. HLA-A alleles were examined by polymerase chain reaction with sequence-specific primers. The frequencies of different HLA-A alleles were compared between the two ethnic groups as well as patients and controls...
October 2018: Journal of Cancer Research and Therapeutics
Hongxia Sun, Ramya P Masand, Shobhanaben Jagdishbhai Patel, Vijayalakshmi Padmanabhan
BACKGROUND: Cervical cancer is caused by high-risk human papillomavirus (hrHPV). Though screening Pap test (PT) has reduced cancer mortality by detecting precursor lesions, there is now a move toward replacing screening PT with hrHPV testing. The aims of this study were to determine hrHPV negative rate in high grade squamous intraepithelial lesion (HSIL) PT in high-risk patients and correlate with histopathology; and to review the hrHPV negative HSIL PT. METHOD: LIS was searched (January 2015-June 2016) for HSIL PT results...
November 2018: Diagnostic Cytopathology
Qiaowen Bu, Sanfeng Wang, Jian Ma, Xiangcheng Zhou, Guiying Hu, Hua Deng, Xiaoli Sun, Xiaoshan Hong, Hengying Wu, Liang Zhang, Xiping Luo
BACKGROUND: To explore the diagnostic value of FAM19A4 methylation in high-risk human papilloma virus (hrHPV)-positive cervical samples from Chinese women for estimating cervical cancer or its precancerous lesions. METHODS: Cervical samples from 215 women infected with high-risk HPV were collected by smear testing. We purposely chose 61 patients with cervical cancer, 57 with high-grade squamous intraepithelial lesions (HSIL), 31 with low-grade squamous intraepithelial lesions (LSIL), and 66 without cervical intraepithelial neoplasia (CIN) after histological confirmation...
November 29, 2018: BMC Cancer
Hongli Liu, Xueting Wei, Zongyu Xie, Xiaojing Wang, Xiaomeng Gong, Wujian Ke, Huachun Zou
Our study aimed to assess the prevalent, incident and persistent infection and clearance of HPV among 19,753 individual women attending the gynecological department at a major comprehensive hospital. HPV16, 52 and 58 ranked top 3 types with the highest prevalence and incidence. The prevalence of high-risk (HR) HPV peaked among women aged 15-19 years, then sharply decreased with age, stabilized among women aged 25-44 years and then surged again among women aged 45 years and older. HR HPV infection were more likely to be prevalent (15...
November 26, 2018: Journal of Medical Virology
L Yu, B Sun, X Liu, J He, H Lou, X Chen, F Tong, L Wei, Y Zhang, W Wei
High-risk human papillomavirus (HPV) is an important pathogenic factor for cervical cancer and understanding the mechanism of HPV gene transcription is important for the prevention and therapy of HPV related cervical cancer. This study aimed to investigate the role of nitric oxide (NO) in the regulation of HPV gene transcription. SiHa cells containing integrated HPV16 were treated with NO donor DETA-NO and cell proliferation and cytotoxicity were determined. HPV gene transcription was detected by real-time PCR...
2018: Acta Virologica
Adrianna Grzelak, Ingrid Polakova, Jana Smahelova, Julie Vackova, Lucie Pekarcikova, Ruth Tachezy, Michal Smahel
Combined immunotherapy constitutes a novel, advanced strategy in cancer treatment. In this study, we investigated immunotherapy in the mouse TC-1/A9 model of human papillomavirus type 16 (HPV16)-associated tumors characterized by major histocompatibility complex class I (MHC-I) downregulation. We found that the induction of a significant anti-tumor response required a combination of DNA vaccination with the administration of an adjuvant, either the synthetic oligodeoxynucleotide ODN1826, carrying immunostimulatory CpG motifs, or α-galactosylceramide (α-GalCer)...
November 21, 2018: International Journal of Molecular Sciences
L Li, L Ding, Y J Lyu, M Wang, C L Liu, Y Han, J T Wang
Objective: To explore the effect of vaginal micro-environment alterations and HPV16 infection and their interaction in the progression of cervical intraepithelial neoplasia. Methods: The participants of this study came from the cervical lesions study cohort in Shanxi province, including 623 women with normal cervical (NC), 303 patients with pathogenically diagnosed low-grade cervical intraepithelial neoplasia (CINⅠ) and 93 patients with pathogenically diagnosed high-grade cervical intraepithelial neoplasia (CINⅡ/Ⅲ)...
November 10, 2018: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Diletta Francesca Squarzanti, Rita Sorrentino, Manuela Miriam Landini, Andrea Chiesa, Sabrina Pinato, Francesca Rocchio, Martina Mattii, Lorenza Penengo, Barbara Azzimonti
BACKGROUND: Despite vaccination and screening measures, anogenital cancer, mainly promoted by HPV16 oncoproteins, still represents the fourth tumor and the second cause of death among women. Cell replication fidelity is the result of the host DNA damage response (DDR). Unlike many DNA viruses that promote their life cycle through the DDR inactivation, HR-HPVs encourage cells proliferation despite the DDR turned on. Why and how it occurs has been only partially elucidated. During HPV16 infection, E6 links and degrades p53 via the binding to the E6AP LXXLL sequence; unfortunately, E6 direct role in the DDR response has not clearly identified yet...
November 16, 2018: Virology Journal
İbrahim Karaca, Mustafa Öztürk, Cihan Comba, Gökhan Demirayak, Şakir Vokan Erdoğan, Murat Hoşgören, İsa Aykut Özdemir
OBJECTIVES: According to the American Society of Colposcopy and Cervical Pathology (ASCCP), cervical cytology patients who test positive for atypical squamous cells of undetermined significance (ASCUS) and the human papilloma virus (HPV) positive are recommended to undergo colposcopies. This study compared the colposcopic biopsy evaluation results of patients with positive cervical cytology results for ASCUS and HPV with regards to their HPV genotypes. METHODS: This study included 179 patients who underwent cervical cancer screening tests between June 2015 and June 2017 and whose results displayed positive cervical cervical cytology results for ASCUS and HPV...
November 16, 2018: Diagnostic Cytopathology
Omar Bauomy Ahmed, Hermann Lage
Delivery of RNAi-mediating shRNA molecules for gene silencing via bacteria, i.e. by transkingdom RNAi (tkRNAi) technology, is suggested to be a powerful alternative technique. In this work, the efficiency of bacterial delivery of shRNAs directed against HPV16-E7-specific mRNA to oral squamous carcinoma cells (OSCCs) was evaluated. E. coli were transfected with a plasmid encoding the inv locus and the Hlya gene to enable the bacteria to enter carcinoma cells and to escape from endocytotic vesicles. The bacterial penetration to the target cells was confirmed by DAPI staining...
November 15, 2018: Cancer Gene Therapy
Martin Bartosik, Ludmila Jirakova, Milan Anton, Borivoj Vojtesek, Roman Hrstka
Major cause of cervical cancer is a persistent infection with high-risk types of human papillomaviruses (HPV). For that reason, HPV testing is now becoming an important addition to standard cytological screening of cervical malignancies in women (known as Pap test). New methods are sought which could offer rapid and inexpensive detection schemes, such as those based on electrochemical (EC) readout. Here, we developed an assay for parallel detection of two most oncogenic high-risk HPV types, HPV16 and HPV18, by combining loop-mediated amplification (LAMP) of template DNA, its separation using magnetic beads and detection with amperometry at carbon-based electrode chips...
December 26, 2018: Analytica Chimica Acta
Petra J Woestenberg, Johannes A Bogaards, Audrey J King, Suzan Leussink, Marianne Ab van der Sande, Christian Jpa Hoebe, Birgit Hb van Benthem
Data on the impact of human papillomavirus (HPV) vaccination on the population HPV prevalence are largely obtained from women. We assessed the impact of the girls-only HPV16/18 vaccination program in the Netherlands that started in 2009, on trends in HPV prevalence among women and heterosexual men, using data from the PASSYON study. In this cross-sectional study, the HPV prevalence among 16- to 24-year-old visitors to sexually transmitted infection clinics was assessed in 2009, 2011, 2013, and 2015. We compared the genital postvaccination HPV prevalence with the prevaccination prevalence (2009) using Poisson GEE models...
November 13, 2018: International Journal of Cancer. Journal International du Cancer
Elisa Anali Camacho-Ureta, Rocío Susana Mendez-Martínez, Salvador Vázquez-Vega, Ulises Osuna Martínez, Rosalinda Sánchez Arenas, Hipólito Castillo-Ureta, Ignacio Osuna Ramírez, Edith Hilario Torres Montoya, Héctor Samuel López Moreno, Alejandro García-Carranca, José Guadalupe Rendón-Maldonado
Background & objectives: Human papillomavirus (HPV) infections play a crucial role in the aetiology of cervical cancer (CC), and HPV16 is the primary viral genotype associated with CC. A number of variants of the HPV16 E6 gene are involved in the progression of CC, differing in their prevalence and biological and biochemical properties. This study was designed to determine the frequency of HPV types 16/18 and to identify the presence of HPV16 E6-variants in asymptomatic Mexican women...
September 2018: Indian Journal of Medical Research
Jiro Sakamoto, Kazuyoshi Shigehara, Kazufumi Nakashima, Shohei Kawaguchi, Takao Nakashima, Masayoshi Shimamura, Mitsuru Yasuda, Taku Kato, Toru Hasegawa, Yoshitomo Kobori, Hiroshi Okada, Takashi Deguchi, Kouji Izumi, Yoshifumi Kadono, Atsushi Mizokami
BACKGROUND AND AIMS: To examine an association between human papillomavirus (HPV) infection and penile cancer among Japanese patients. METHODS: Thirty-four patients with penile cancer were enrolled in this study. DNA was extracted from paraffin-embedded tumor-tissue samples, and HPV-DNA tests and genotyping were performed. In all of samples, in situ hybridization (ISH) was performed to locate HPV-DNA in tumor tissue; expression levels of p16-INK4a, mini-chromosome maintenance protein-7(mcm-7), HPV-L1, and Ki-67 were analyzed using immunohistochemical methods...
November 10, 2018: International Journal of Infectious Diseases: IJID
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