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Kalliopi I Pappa, Polyxeni Christou, Amarildo Xholi, George Mermelekas, Georgia Kontostathi, Vasiliki Lygirou, Manousos Makridakis, Jerome Zoidakis, Nicholas P Anagnou
The available therapeutic approaches for cervical cancer can seriously affect the fertility potential of patient; thus, there is a pressing requirement for less toxic and targeted therapies. The membrane proteome is a potential source of therapeutic targets; however, despite the significance of membrane proteins in cancer, proteomic analysis has been a challenging task due to their unique biochemical properties. The aim of the present study was to develop an efficient membrane protein enrichment protocol, and to the best of our knowledge, to compare for the first time the expression pattern of membrane proteins of one normal cell line, HCK1T, and three cervical cancer cell lines, C33A, a human papilloma virus (HPV)-negative cell line, and two HPV-positive cell lines, SiHa (HPV16+) and HeLa (HPV18+)...
August 7, 2018: International Journal of Oncology
Sujita Khanal, Brian S Shumway, Maryam Zahin, Rebecca A Redman, John D Strickley, Patrick J Trainor, Shesh N Rai, Shin-Je Ghim, Alfred Bennett Jenson, Joongho Joh
This study evaluated the integration and methlyation of human papillomavirus type 16 (HPV16) in head and neck squamous cell carcinoma (HNSCC) and its oral precursor, high-grade oral epithelial dysplasia (hgOED). Archival samples of HPV16-positive hgOED ( N = 19) and HNSCC ( N = 15) were evaluated, along with three HNSCC (UMSCC-1, -47 and -104) and two cervical cancer (SiHa and CaSki) cell lines. HgOED cases were stratified into three groups with increasing degrees of cytologic changes (mitosis, karyorrhexis and apoptosis)...
July 13, 2018: Oncotarget
Xiao Bao, Aimee L Hanson, Margaret M Madeleine, Sophia S Wang, Stephen M Schwartz, Felicity Newell, Ulrika Pettersson-Kymmer, Kari Hemminki, Sven Tiews, Winfried Steinberg, Janet S Rader, Felipe Castro, Mahboobeh Safaeian, Eduardo L Franco, François Coutlée, Claes Ohlsson, Adrian Cortes, Mhairi Marshall, Pamela Mukhopadhyay, Katie Cremin, Lisa G Johnson, Suzanne M Garland, Sepehr N Tabrizi, Nicolas Wentzensen, Freddy Sitas, Cornelia Trimble, Julian Little, Maggie Cruickshank, Ian H Frazer, Allan Hildesheim, Matthew A Brown, Emma L Duncan, YingPu Sun, Paul J Leo
Background: Cervical cancer is the fourth most common cancer in women, and we recently reported human leukocyte antigen (HLA) alleles showing strong associations with cervical neoplasia risk and protection. HLA ligands are recognised by killer immunoglobulin-like receptors (KIRs) expressed on a range of immune cell subsets, governing their proinflammatory activity. We hypothesized that the inheritance of particular HLA-KIR combinations would increase cervical neoplasia risk. Methods: Here, we used HLA and KIR dosages imputed from SNP genotype data from 2,143 cervical neoplasia cases and 13,858 healthy controls of European decent...
August 7, 2018: Journal of Infectious Diseases
A Leeman, M Del Pino, L Marimon, A Torné, J Ordi, B Ter Harmsel, C J L M Meijer, D Jenkins, F J Van Kemenade, W G V Quint
Cervical screening aims to identify women with high-grade squamous intraepithelial lesion/cervical intraepithelial neoplasia 2-3 (HSIL/CIN2-3) or invasive cervical cancer (ICC). Identification of women with severe premalignant lesions or ICC (CIN3+) could ensure their rapid treatment and prevent overtreatment. We investigated high-risk human papillomavirus (hrHPV) detection with genotyping and methylation of FAM19A4/miR124-2 for detection of CIN3+ in 538 women attending colposcopy for abnormal cytology. All women had an additional cytology with hrHPV testing (GP5+/6+-PCR-EIA+), genotyping (HPV16/18, HPV16/18/31/45), and methylation analysis (FAM19A4/miR124-2) and at least one biopsy...
August 11, 2018: International Journal of Cancer. Journal International du Cancer
Michelle I Silver, Jeff Andrews, Charles K Cooper, Julia C Gage, Michael A Gold, Michelle J Khan, L Stewart Massad, Valentin Parvu, Rebecca B Perkins, Mark Schiffman, Katie M Smith, Nicolas Wentzensen
OBJECTIVE: To calculate pooled risk estimates for combinations of cytology result, human papillomavirus (HPV) 16/18 genotype and colposcopy impression to provide a basis for risk-stratified colposcopy and biopsy practice. DATA SOURCE: A PubMed search was conducted on June 1, 2016, and a search was conducted on June 9, 2018, using key words such as "uterine cervical neoplasms," "cervical cancer," "mass screening," "early detection of cancer," and "colposcopy...
August 6, 2018: Obstetrics and Gynecology
Ruiyi Zhang, Xuefei Ge, Ke You, Yanli Guo, Hongyan Guo, Yanjie Wang, Li Geng
AIM: The goal of this study was to analyze the specificity of p16/Ki67 dual staining in the detection of high-grade cervical lesions. METHODS: A total of 223 patients with an average age of 39 years old were enrolled in this study. All samples were analyzed by p16/Ki67 immunocytochemical dual staining, liquid-based cytology and high-risk human papillomavirus (HR-HPV) test. Diagnosis of each patient was verified by histopathological test. RESULTS: The specificity of p16/Ki67 dual staining was 68...
August 9, 2018: Journal of Obstetrics and Gynaecology Research
C W Easterly, F Alarid-Escudero, E A Enns, S Kulasingam
BACKGROUND: Sexual mixing between heterogeneous population subgroups is an integral component of mathematical models of sexually transmitted infections (STIs). This study compares the fit of different mixing representations to survey data and the impact of different mixing assumptions on the predicted benefits of hypothetical human papillomavirus (HPV) vaccine strategies. METHODS: We compared novel empirical (data-driven) age mixing structures with the more commonly-used assortative-proportionate (A-P) mixing structure...
August 6, 2018: Vaccine
Bigyan Mainali, Matthew B Schabath, Staci L Sudenga, Cynthia Ye, Howard W Wiener, Luisa L Villa, Anna R Giuliano, Sadeep Shrestha
OBJECTIVES: While most human papillomavirus (HPV) infection clears on its own, persistent HPV infection can cause genital warts and anal, penile and oropharyngeal cancers in men. We conducted genetic analysis in a sub-cohort of the HPV infection in men (HIM) study to test the hypothesis that differences in host genes influence HPV persistence in men. METHODS: Baseline and longitudinal genital HPV status at the genitals was measured every 6-months using the Linear Array assay amplified HPV L1 gene fragment using the PGMY09/11 L1 consensus primer system...
August 6, 2018: Papillomavirus Research
Sharbadeb Kundu, Vijayalakshmi Ramshankar, Akalesh Kumar Verma, Soundara Viveka Thangaraj, Arvind Krishnamurthy, Rajeev Kumar, Ravi Kannan, Sankar Kumar Ghosh
Southeast Asia, especially India, is well known for the highest use of smokeless tobacco. These products are known to induce oral squamous cell carcinoma. However, not all long-term tobacco-chewers develop oral squamous cell carcinoma. In addition, germline variants play a crucial role in susceptibility, prognosis, development, and progression of the disease. These prompted us to study the genetic susceptibility to oral squamous cell carcinoma among the long-term tobacco-chewers. Here, we presented a retrospective study on prolonged tobacco-chewers of Northeast India to identify the potential protective or risk-associated germline variants in tobacco-related oral squamous cell carcinoma along with HPV infection...
August 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Michelle Ainouze, Pauline Rochefort, Peggy Parroche, Guillaume Roblot, Issam Tout, François Briat, Claudia Zannetti, Marie Marotel, Nadege Goutagny, Philip Auron, Alexandra Traverse-Glehen, Aude Lunel-Potencier, Francois Golfier, Murielle Masson, Alexis Robitaille, Massimo Tommasino, Christine Carreira, Thierry Walzer, Thomas Henry, Katia Zanier, Gilles Trave, Uzma Ayesha Hasan
Human papillomavirus type 16 (HPV16) and other oncoviruses have been shown to block innate immune responses and to persist in the host. However, to avoid viral persistence, the immune response attempts to clear the infection. IL-1β is a powerful cytokine produced when viral motifs are sensed by innate receptors that are members of the inflammasome family. Whether oncoviruses such as HPV16 can activate the inflammasome pathway remains unknown. Here, we show that infection of human keratinocytes with HPV16 induced the secretion of IL-1β...
August 8, 2018: PLoS Pathogens
Patrick Nyman, Darya Buehler, Paul F Lambert
PURPOSE: Head and neck squamous cell carcinoma (HNSCC), a common cancer worldwide, is etiologically associated with tobacco use, high alcohol consumption and high risk human papillomaviruses (HPV). The Notch signaling pathway, which is involved in cell differentiation decisions with differential downstream targets and effects depending on tissue type and developmental stage, has been implicated in human HNSCC. Notch1 is among the most frequently mutated genes in both HPV-positive and HPV-negative HNSCC...
August 7, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Ilir Agalliu, Zigui Chen, Tao Wang, Richard B Hayes, Neal D Freedman, Susan M Gapstur, Robert D Burk
BACKGROUND: Several studies have examined association between human papillomaviruses (HPVs) and esophageal cancer, but results have been inconsistent. This is the first prospective study to investigate associations between alpha, beta and gamma HPV detection in the oral cavity and risk of esophageal cancer. METHODS: We conducted a nested case-control study among 96,650 cancer-free participants in the American Cancer Society Cancer Prevention Cohort and the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial...
August 7, 2018: Cancer Epidemiology, Biomarkers & Prevention
Luana L S Rodrigues, Mariza G Morgado, Vikrant V Sahasrabuddhe, Vanessa S De Paula, Nathália S Oliveira, Elena Chavez-Juan, Diane M Da Silva, W Martin Kast, Alcina F Nicol, José H Pilotto
OBJECTIVE: We evaluated acceptability of cervico-vaginal self-collection (CVSC) and prevalence of human papillomavirus (HPV) in Human immunodeficiency virus (HIV)-infected and HIV-uninfected women living in the Tapajós region, Amazon, Brazil. METHODS: Cross-sectional study recruited 153 non-indigenous women (HIV-uninfected, n = 112 and HIV-infected, n = 41) who voluntarily sought assistance in health services. Peripheral blood for HIV screening and cervical scraping (CS) for HPV detection were collected...
August 4, 2018: Gynecologic Oncology
Eduardo Lazcano-Ponce, Leticia Torres-Ibarra, Aurelio Cruz-Valdez, Jorge Salmerón, Tonatiuh Barrientos-Gutiérrez, Javier Prado-Galbarro, Margaret Stanley, Nubia Muñoz, Rolando Herrero, Mauricio Hernández-Ávila
Background: There is limited data regarding the duration of immunity induced by different HPV vaccination schedules and the immunogenicity of a booster dose of both bivalent (bHPV) or quadrivalent (qHPV) HPV vaccine. Methods: Follow-up of a non-randomized clinical trial to evaluate the 5-years antibody persistence of the bHPV in girls (9-10 years) and women (18-24 years). Non-inferiority of the two- versus three-dose schedule among girls was evaluated at months 54 (n=639) and 64 (n=990)...
August 1, 2018: Journal of Infectious Diseases
Gad Murenzi, Jean-Claude Dusingize, Theogene Rurangwa, Jean d'Amour Sinayobye, Athanase Munyaneza, Anthere Murangwa, Thierry Zawadi, Tiffany Hebert, Pacifique Mugenzi, Adebola Adedimeji, Leon Mutesa, Kathryn Anastos, Philip E Castle
INTRODUCTION: The optimal method(s) for screening HIV-infected women, especially for those living in sub-Saharan Africa, for cervical precancer and early cancer has yet to be established. METHODS AND ANALYSIS: A convenience sample of >5000 Rwandan women, ages 30-54 years and living with HIV infection, is being consented and enroled into a cross-sectional study of cervical cancer screening strategies. Participants are completing an administered short risk factor questionnaire and being screened for high-risk human papillomavirus (hrHPV) using the Xpert HPV assay (Cepheid, Sunnyvale, California, USA), unaided visual inspection after acetic acid (VIA) and aided VIA using the Enhanced Visual Assessment (EVA) system (Mobile ODT, Tel Aviv, Israel)...
August 5, 2018: BMJ Open
Saloua Kahla, Samia Hammami, Lotfi Kochbati, Mohamed Badis Chanoufi, Ridha Oueslati
Specific genetic mutations in human papillomavirus type 16 (HPV16) DNA are considered important in cervical lesion progression. This study analyzes to what extent radiotherapy treatment contributes to viral DNA mutation in cervical cell carcinomas, and the biological significance of these mutations. Serial tumor tissue, including 44 cervical cancer samples, collected before and after radiotherapy, and 52 biopsies with benign cervices, were tested and analyzed for the presence of HPV16, and for the integrity of the E2 gene...
August 3, 2018: Infection, Genetics and Evolution
Z Zhang, D Zhang, B B Xiao, R Zhang, H H Bai, H Y Dong, H Bi, Z H Liu
Objective: To understand characteristics of vaginal cervical microbiota in high-risk HPV (hrHPV) infected women and to uncover the relationship between hrHPV infection and vaginal cervical microbiota. Methods: All participants were randomly selected from Peking University First Hospital from September to October of 2017, including 5 subjects of control group, 5 cases of HPV16/18 group, 5 cases of other hrHPV infected group and 3 cases of cervical squamous carcinoma group. All subjects were required to fill in a questionnaire, and cervical and vaginal discharges were separately collected for microscopic examination and new generation sequencing targeting the variable region (V3-V4) of bacterial 16S rRNA gene...
July 25, 2018: Zhonghua Fu Chan Ke za Zhi
Lilly Y W Bourguignon, Christine Earle, Marisa Shiina
Head and neck squamous cell carcinoma (HNSCC) is a malignancy that often involves the oral cavity, pharynx, larynx, or paranasal sinuses. There is a compelling evidence of the human papilloma virus including HPV16 E6 oncogene drives cell transformation and oncogenic processes of HPV positive (HVP+) HNSCC [in particular, Oropharyngeal Squamous Cell Carcinoma (OPSCC)]. In this study, we determined that human OPSCC-derived, HPV16 E6+ cells (UMSCC-104 and UMSCC-47 cell lines) express CD44 and a regulatory transcription factor, c-Jun...
August 2, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Yan-Xiang Cheng, Gan-Tao Chen, Xiao Yang, Yan-Qing Wang, Li Hong
The objectives of this study were to investigate the effects of the CRISPR/Cas9 system mediated by the HPV pseudotype virus on SiHa cytobiology behavior by cutting the HPV16 E6 gene selectively and to explore the role of this system in the treatment of cervical cancer. After designing specific gRNA sequences targeting HPV16 E6, generating hCas9-EGFP and E6-gRNA-RFP plasmids, and preparing the pseudovirus of HPV16 carrying E6-gRNA and Cas9 plasmids, we determined the titer of the pseudotype virus using the TCID50 method...
April 2018: Current medical science
B Medeiros-Fonseca, V F Mestre, B Colaço, M J Pires, T Martins, R M Gil da Costa, M J Neuparth, R Medeiros, Magda S S Moutinho, Maria Inês Dias, Lillian Barros, M M S M Bastos, L Félix, C Venâncio, Isabel C F R Ferreira, L M Antunes, P A Oliveira
Cancers induced by human papillomavirus (HPV) infection remain a significant public health threat, fueling the study of new therapies. Laurel (Laurus nobilis) compounds and extracts recently showed in vitro activity against HPV-transformed cell lines. This work aims to evaluate the in vivo efficacy and hepatic toxicity of a laurel extract in a transgenic mouse model of HPV16-induced cancer. The extract was administered in drinking water (20 mg per animal per day) for three consecutive weeks, using four experimental groups (n = 10) (group I: HPV16-/- without treatment, group II: treated HPV16-/-, group III: HPV16+/- without treatment and group IV: treated HPV16+/-)...
August 1, 2018: Food & Function
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