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Sijie Fang, Yazhuo Huang, Ningjian Wang, Shuo Zhang, Sisi Zhong, Yinwei Li, Jing Sun, Xingtong Liu, Yang Wang, Ping Gu, Bin Li, Huifang Zhou, Xianqun Fan
Context: Unique features of local immunity in thyroid-associated ophthalmopathy (TAO) may affect disease progression. Objective: To investigate the association between the orbital immune microenvironment and TAO development. Design/Setting/Participants: TAO and control orbital connective tissues were collected. Main Outcome Measures: Single-cell sequencing examined orbital lymphocytic infiltrates. Multi-color flow cytometry explored the phenotypes of different cell subsets and in vitro models for cell functional studies...
December 3, 2018: Journal of Clinical Endocrinology and Metabolism
Varsha I Shah, Gareth L Rowlands, Ian W Thompson, Vaiyapuri P Sumathi, W Glenn McCluggage
Angiosarcomas of the female genital tract are rare and primary angiosarcoma of the cervix is extremely rare with only one prior case report. We report a case of a primary cervical angiosarcoma in a 43-yr-old woman who presented with heavy vaginal bleeding. Cervical biopsy and subsequent radical hysterectomy showed a malignant vascular tumor which was composed of spindled and epithelioid cells and formed abortive vascular channels. Immunohistochemically, the tumor cells were diffusely positive for CD31, CD34, ERG, and cyclin D1 and focally positive for D2-40...
December 3, 2018: International Journal of Gynecological Pathology
Walid Warda, Fabrice Larosa, Mathieu Neto Da Rocha, Rim Trad, Eric Deconinck, Ziad Fajloun, Cyril Faure, Denis Caillot, Marius Moldovan, Severine Valmary-Degano, Sabeha Biichle, Etienne Daguindau, Francine Garnache-Ottou, Sebastien Tabruyn, Olivier Adotévi, Marina Deschamps, Christophe Ferrand
Chronic myeloid leukemia (CML) is a chronic disease resulting in myeloid cell expansion through expression of the BCR-ABL1 fusion transcript. Tyrosine kinase inhibitors (TKI) have significantly increased survival of CML patients, and deep responders may consider stopping the treatment. However, more than 50% of patients relapse and restart TKI, subsequently suffering unknown toxicity. Because CML is a model immune system-sensitive disease, we hypothesize that chimeric antigen receptor (CAR) T cells targeting interleukin-1 receptor-associated protein (IL-1RAP) in quiescent CML stem cells may offer an opportunity for a permanent cure...
December 4, 2018: Cancer Research
Yunpeng Shi, Chengrui Nan, Zhongjie Yan, Liqiang Liu, Jingjing Zhou, Zongmao Zhao, Depei Li
Objective: The human umbilical cord mesenchymal stem cells (hUMSCs) are characterized with the potential ability to differentiate to several types of cells. Edaravone has been demonstrated to prevent the hUMSCs from the oxidative damage, especially its ability in antioxidative stress. We hypothesized that Edaravone induces the hUMSCs into the neuron-like cells. Methods: The hUMSCs were obtained from the human umbilical cord tissue. The differentiation of hUMSCs was induced by Edaravone with three different doses: 0...
2018: Stem Cells International
Zbigniew Rudzki, Richard Lovell, Ben Miller, Vidhya Murthy, Shankara Paneesha, Guy Pratt, Neil Smith
A 56-year-old female presented with popliteal venous thrombosis, splenomegaly, and sclerotic bone lesions. Bone marrow biopsy showed fibrosis, proliferation of abnormal blood vessels and lymphatics, bone remodelling, and no significant changes in haematopoietic elements. Following a relatively indolent initial clinical course, one year later she rapidly deteriorated and died of respiratory failure associated with widespread disease. Ingrowth of podoplanin+ lymphatics mixed with CD34+/podoplanin- blood vessels into the bone marrow is a new finding and may be a unique feature of this disease...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
Pier P Piccaluga, Stefania Paolini, Mohsen Navari, Maryam Etebari, Giuseppe Visani, Stefano Ascani
Myeloid sarcomas (MS) are tumors composed by myeloid elements and developing outside bone marrow. The prognosis is overall poor, only stem cell transplantation being consistently reposted as a potentially curative approach. In this study we explored whether microvessel density, a biomarker of angiogenesis, might be relevant in MS. We studied 60 MS, 24 acute myeloid leukemia, 5 normal bone marrow samples and 2 cases of extramedullary hemopoiesis in patients without evidence of hematological malignancy. We used immunohistochemistry (anti-CD34) to identify and quantify micro-vessel density (MVD) and micro-vessel grading (MVG)...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
Mariusz Kobek, Zbigniew Jankowski, Janusz Szala, Zbigniew Gąszczyk-Ożarowski, Artur Pałasz, Rafał Skowronek
In our previous work, we have discussed the importance of neurofilaments in determination of the age of brain contusions. The purpose of this paper is to examine a possibility of angiogenesis-related assessment of the age of brain contusions by means of morphometric analysis of the CD34 expression in the contused sites. The researched material comprised 90 cases divided into nine groups according to the time of death: immediately on the spot, 12 hours, 24 hours, 2 days, 3 days, 4 days, 5 days, 6 days, and 7 days after the head trauma...
2018: Folia Neuropathologica
Wei Hu, Jian Jiang, Feng Yang, Jun Liu
INTRODUCTION: The impact of bone marrow-derived mesenchymal stem cells (BM-MSCs) on neovascularisation in rats with craniocerebral injury (CI) was investigated. MATERIAL AND METHODS: The rate CI model was established by the free-fall method, and then rats were randomly divided into a transplanted group (EG) and a control group (CG). Rats in the transplanted group were injected with BM-MSCs in the lateral ventricle, while those in the control group were injected with normal saline...
2018: Folia Neuropathologica
Sant Prakash Kataria, Namita Bhutani, Sanjay Kumar, Gajender Singh, Rajeev Sen, Ishwar Singh
INTRODUCTION: Solitary fibrous tumor (SFT) is a rare neoplasm of mesenchymal origin that occurs most often in the visceral pleura. It rarely occurs in the central nervous system (CNS). If they occur intracranially, they are extra-axially located and develop from the meninges. CASE REPORT: Herein, we present a case of SFT in a 45 years old female. MR imaging showed isointense to hypointense signal intensity, and marked enhancement. Microscopically, the tumors showed characteristic "patternless-pattern" architecture...
November 27, 2018: International Journal of Surgery Case Reports
Na Li, Chu Liu, Guifen Ma, Yujen Tseng, Duyi Pan, Jie Chen, Feng Li, Xiaoqing Zeng, Tiancheng Luo, Shiyao Chen
BACKGROUND AND AIMS: pathological angiogenesis plays an important role in the progression of chronic liver diseases. Asparaginyl endopeptidase (AEP) participates in tumor angiogenesis and was recently shown to be associated with liver fibrosis. This study aimed to explore the effect of AEP on liver sinusoidal endothelial cell (LSECs) angiogenesis and determine the underlying mechanism. METHODS: cultured LSECs were infected with lentiviruses in order to suppress AEP expression (AEP-KD1, AEP-KD2)...
December 3, 2018: Revista Española de Enfermedades Digestivas
Changsong Qi, Fang Pan, Jian Li, Yanyan Li, Jing Gao, Lin Shen
OBJECTIVE: To investigate the biological behavior characteristics of gastrointestinal stromal tumors (GIST) with PDGFRA mutation and the patients' survival, and elucidate the efficacy of imatinib therapy. METHODS: Patients with PDGFRA D842V and non-D842V mutations were screened from a database of 1163 patients with GIST who were treated at Peking University Cancer Hospital from 2003 to 2018. Clinicopathological data of these patients were collected, including gender, age, tumor size, mitotic phase, primary position, metastatic site, and expressions of CD117, CD34, DOG-1...
November 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Priya T Rajan, T N Suresh, T S Rajashekar
Background: Psoriasis is a common chronic and immune-mediated skin disorder having a significant impact on the patient's quality of life. An analysis of the role of angiogenic vascular endothelial growth factor (VEGF) expression and microvessel density in psoriatic skin lesions may help in better understanding of the disease pathogenesis. The aim of this study was to examine the expression of vascular endothelial growth factor (VEGF) and microvessel density using CD34 antibodies in psoriatic skin lesions by immunohistochemical examination using normal skin of healthy individuals as controls...
November 2018: Indian Dermatology Online Journal
Jenna N Harvestine, Hakan Orbay, Jonathan Y Chen, David E Sahar, J Kent Leach
Lipoaspirates contain a readily accessible heterogeneous cell source for use in bone regeneration collectively referred to as the stromal vascular fraction (SVF). However, the osteogenic potential of SVF is inferior to other progenitor cell populations, thereby requiring alternative strategies to potentiate its effective use in cell-based therapies of bone repair. Cell-secreted extracellular matrix (ECM) is a promising substrate to guide cell phenotype or for use in biomaterial design, yet the instructional capacity of ECMs produced by various cell types is unknown...
June 28, 2018: Journal of Materials Chemistry. B, Materials for Biology and Medicine
Yanling Zhang, Heng Tang, Huaiyuan Hu, Xiang Yong
Primary leiomyomas of the thyroid are very rare. We here report a case of a 53-year-old woman with a painless mass at the right thyroid, revealed by physical examination. The patient underwent a lobectomy. Frozen sections showed a spindle cell tumor of the thyroid gland. The nuclei of some of the tumor cells were obviously enlarged and deeply stained. Pseudocapsule invasion was observed in small foci. Samples showed neither mitosis nor necrosis and the nature of the tumor was difficult to determine. Paraffin sections showed a well-circumscribed nodular composed of intersecting fascicles of spindled to slightly epithelioid cells with eosinophilic cytoplasm and blunt-ended, cigar-shaped nuclei...
2018: Clinical Medicine Insights. Oncology
Phillip Scheinberg
Since the approval of horse antithymocyte globulin (ATG) decades ago, there was a long hiatus in therapies with activity in severe aplastic anemia (SAA). This scenario changed in 2014 when eltrombopag, a thrombopoietin receptor agonist, was approved for SAA after an insufficient response to initial immunosuppressive therapy (IST). The basis for this approval was the observation of single-agent activity of eltrombopag in this patient population, where 40% to 50% recovered blood counts at times involving >1 lineage...
November 30, 2018: Hematology—the Education Program of the American Society of Hematology
M Sulce, L Marconato, M Martano, S Iussich, A Dentini, M Melega, B Miniscalco, F Riondato
Mast cell tumors (MCT) are among the most frequent tumors in dogs, but studies regarding canine mast cell immunophenotype are lacking. The aim of this study was to assess the feasibility of flow cytometric analysis of MCTs, to describe canine MCTs immunophenotype(s), and to evaluate the ability of flow cytometry to detect mast cells in lymph node aspirates. Thirty-four primary canine MCTs and 12 draining lymph nodes were evaluated regarding the expression of CD117, IgE, CD11b, CD18, CD44, CD34, CD25 and CD45...
December 2018: Veterinary Journal
Ege Ülgen, Pınar Kuru Bektaşoğlu, M Aydın Sav, Özge Can, Ayça Erşen Danyeli, Deniz Baycın Hızal, M Necmettin Pamir, Koray Özduman
BACKGROUND: Meningiomas are heterogenous with differences in anatomical, histopathological and clinical characteristics. Such spatial variability in meningioma biology is thought to be due to differences in expression
of critical developmental regulators. We hypothesized that variability in meningioma biology follows gradients as in embryology and tested a cohort of 366 meningiomas for histopathological and immunohistochemical gradients. METHODS: Medical records of 366 patients operated for meningiomas between 2003 and 2016 were retrospectively analyzed for age, gender, anatomical localization, recurrence-free survival, overall survival, histopathological diagnosis and immunohistochemistry findings for six markers: epithelial membrane antigen (EMA), progesterone receptor (PR), CD34, S100, P53 and Ki-67 labeling index (Ki-67)...
November 29, 2018: World Neurosurgery
Jia-Xin Liu, Wei-Na Ma, Yi Tan, Yong-Qing Liang, Wen-Jie Yin, Jian Cen, You-Zhang Huang, Jian-Liang Shen
OBJECTIVE: To explore the maintaining measures for the vitality of hematopoietic stem cells (HSC) in vitro, so as provide technical support for ultra long distance transport of HSC collected from unrelated donors. METHODS: Peripheral blood hematopoietic stem cells (PBHSC) were treated by different methods according to various groups, then stored at 4 ℃ in the refrigerator. The percentage of CD34+ cells, relative cell activity, relative cell proliferation rate, relative colony-forming rate, oxygen fraction and intracellular reactive oxygen species (ROS) were detected at 0, 24, 48 and 72 h after storage of PBHSC respectively...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Denis Tvorogov, Daniel Thomas, Nicholas P D Liau, Mara Dottore, Emma F Barry, Maya Lathi, Winnie L Kan, Timothy R Hercus, Frank Stomski, Timothy P Hughes, Vinay Tergaonkar, Michael W Parker, David M Ross, Ravindra Majeti, Jeffrey J Babon, Angel F Lopez
Treatment of patients with myelofibrosis with the type I JAK (Janus kinase) inhibitor ruxolitinib paradoxically induces JAK2 activation loop phosphorylation and is associated with a life-threatening cytokine-rebound syndrome if rapidly withdrawn. We developed a time-dependent assay to mimic ruxolitinib withdrawal in primary JAK2V617F and CALR mutant myelofibrosis patient samples and observed notable activation of spontaneous STAT signaling in JAK2V617F samples after drug washout. Accumulation of ruxolitinib-induced JAK2 phosphorylation was dose dependent and correlated with rebound signaling and the presence of a JAK2V617F mutation...
November 2018: Science Advances
Michaela Bártů, Pavel Dundr, Kristýna Němejcová, Petra Prokopová, Iva Zambo, Štěpán Černý
Here we report on the case of a 61-year-old female with an accidental finding of intravascular fasciitis (IVF) affecting the ascending aorta in a specimen resected due to an acute aortic dissection. No infiltrative process of the aorta or surrounding soft tissues was grossly apparent. Microscopically, the lesion had poorly defined margins and was composed of plump spindle- and oval-shaped cells set in an abundant myxoid stroma. Immunohistochemically, cytoplasmic positivity for β-catenin was observed and about 25% of cells were positive for calponin...
2018: Ceskoslovenská Patologie
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