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Kyu Yun Jang, Ho Sung Park, Kyoung Min Kim, Ho Lee, Chan Young Kim
Plexiform fibromyxoma of the stomach is a very rare mesenchymal tumor found exclusively in the antrum or pylorus and characterized by plexiform growth pattern and abundant myxoid/fibromyxoid stroma. We present both cytological and histological features of gastric plexiform fibromyxoma in a 47-year-old woman. Cytological smears revealed moderate cellularity with loose clusters consisted of spindle cells, which were arranged in no specific pattern, abundant myxoid stroma as well as thin-walled blood vessels. The spindle cells revealed minimal pleomorphism and had scant cytoplasm and slender nuclei with fine chromatin and indistinct nucleoli...
August 13, 2018: Cytopathology: Official Journal of the British Society for Clinical Cytology
Rudolf A Werner, Hiroshi Wakabayashi, Jochen Bauer, Claudia Schütz, Christina Zechmeister, Nobuyuki Hayakawa, Mehrbod S Javadi, Constantin Lapa, Roland Jahns, Süleyman Ergün, Valerie Jahns, Takahiro Higuchi
Aims: Although mortality rate is very high, diagnosis of acute myocarditis remains challenging with conventional tests. We aimed to elucidate the potential role of longitudinal 2-Deoxy-2-18F-fluoro-D-glucose (18F-FDG) positron emission tomography (PET) inflammation monitoring in a rat model of experimental autoimmune myocarditis. Methods and results: Autoimmune myocarditis was induced in Lewis rats by immunizing with porcine cardiac myosin emulsified in complete Freund's adjuvant...
August 8, 2018: European Heart Journal Cardiovascular Imaging
Akihiro Miura, Eigo Kojima, Kouichi Tashiro, Takehiro Tajima, Makiko Matsumura, Nobuo Yanagisawa, Yoshihiro Ohno
A 67-year-old man visited our hospital with an enlarging abdominal mass several months after he had first noticed his symptoms. An elastic firm tumor was palpated on the left side of the abdomen upon physical examination. The blood test results were normal. Contrast-enhanced computed tomography of the abdomen revealed a 10-cm-diameter homogeneous low-density cystic tumor located at the dorsal portion of the gastric corpus. Enhancement of a few net-like structures was noted, but most of the lesion was not enhanced...
2018: Nihon Shokakibyo Gakkai Zasshi, the Japanese Journal of Gastro-enterology
Mor Ngom, Suzan Imren, Tobias Maetzig, Jennifer E Adair, David J H F Knapp, Jalila Chagraoui, Iman Fares, Marie-Eve Bordeleau, Guy Sauvageau, Philippe Leboulch, Connie Eaves, Richard Keith Humphries
Enhanced gene transfer efficiencies and higher yields of transplantable transduced human hematopoietic stem cells are continuing goals for improving clinical protocols that use stemcell-based gene therapies. Here, we examined the effect of the HSC agonist UM171 on these endpoints in both in vitro and in vivo systems. Using a 22-hr transduction protocol, we found that UM171 significantly enhances both the lentivirus-mediated transduction and yield of CD34+ and CD34+ CD45RA- hematopoietic cells from human cord blood to give a 6-fold overall higher recovery of transduced hematopoietic stem cells, including cells with long-term lympho-myeloid repopulating activity in immunodeficient mice...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
Pramod Nepal, Shinichiro Mori, Yoshiaki Kita, Kan Tanabe, Kenji Baba, Yasuto Uchikado, Hiroshi Kurahara, Takaaki Arigami, Masahiko Sakoda, Kosei Maemura, Shoji Natsugoe
BACKGROUND: Rectal gastrointestinal stromal tumor (GIST) is a very rare tumor of gastrointestinal tract. Surgical management of rectal GIST requires special attention for preserving of anal and urinary functions. Transanal minimal invasive surgery (TAMIS) is a well-developed minimally invasive technique for local excision of benign and early malignant rectal tumors; however, the application of TAMIS for rectal GIST is rarely and inadequately reported. We report the novel application of TAMIS for rectal GIST with considerations for anal and urinary functions...
August 11, 2018: World Journal of Surgical Oncology
João Augusto Vianna Goulart-Filho, Victor Angelo Martins Montalli, Fabrício Passador-Santos, Andresa Borges Soares, Ney Soares de Araújo, Vera Cavalcanti de Araújo
Considered as an aggressive counterpart of central ossifying fibroma (OF), juvenile ossifying fibroma (JOF) is a benign fibro-osseous neoplasm characterized by an unpredictable destructive behavior, elevated morbidity, mutilating treatment and high potential for local recurrences. The aim of this study is to compare the analysis for cell proliferation and vascular markers between JOF and OF. Cell proliferation index was measured by Ki-67 and Mcm-2 expression and microvessel density (MVD) was obtained by the immunoexpression of CD34/CD105...
August 4, 2018: Annals of Diagnostic Pathology
Jun Seong Lee, Se-Jin Jeong, Sinai Kim, Lorraine Chalifour, Tae Jin Yun, Mohammad Alam Miah, Bin Li, Abdelilah Majdoubi, Antoine Sabourin, Tibor Keler, Jean V Guimond, Elie Haddad, Eui-Young Choi, Slava Epelman, Jae-Hoon Choi, Jacques Thibodeau, Goo Taeg Oh, Cheolho Cheong
Ischemic myocardial injury results in sterile cardiac inflammation that leads to tissue repair, two processes controlled by mononuclear phagocytes. Despite global burden of cardiovascular diseases, we do not understand the functional contribution to pathogenesis of specific cardiac mononuclear phagocyte lineages, in particular dendritic cells. To address this limitation, we used detailed lineage tracing and genetic studies to identify bona fide murine and human CD103+ conventional dendritic cell (cDC)1s, CD11b+ cDC2s, and plasmacytoid DCs (pDCs) in the heart of normal mice and immunocompromised NSG mice reconstituted with human CD34+ cells, respectively...
August 10, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Rossana Domenis, David Pilutti, Maria Orsaria, Stefania Marzinotto, Veronica Candotti, Giulia Bosisio, Michela Bulfoni, Maria Elisabetta Ruaro, Carla Di Loreto, Vincenzo Della Mea, Eleonora Toffoletti, Ambrogio P Londero, Laura Mariuzzi, Giorgia Gri
S100A4 protein is expressed in fibroblasts during tissue remodelling and in cancer stem cells and it induces the metastatic spread of tumor cells. In mast cells (MCs) S100A4 have been found in some pathological conditions, but its function in normal MCs remains to be described. The purpose of this study was to characterize the cellular localization of the S100A4 protein in MCs of human tissues with inflammatory or tumor disorders and, to determine the consequence of reducing its expression in MC response. We found that tissue resident MCs stained positive to S100A4...
August 4, 2018: Cellular Immunology
Jang Yoo, Bom Sahn Kim, Hai-Jeon Yoon
OBJECTIVE: This study aimed to demonstrate the clinical significance of total lesion glycolysis (TLG) of primary breast cancer using 18 F-FDG PET/CT to predict axillary lymph node (ALN) metastasis in invasive ductal breast cancer (IDC) with a clinically negative axillary lymph node (cN-ALN). METHODS: 135 patients, newly diagnosed with IDC with C N-ALN between July 2016 and October 2017, were retrospectively enrolled. We estimated primary tumor PET/CT parameters including the maximum standard uptake value (SUVmax ), metabolic tumor volume (MTV), and TLG, as well as clinicopathologic findings...
August 9, 2018: Annals of Nuclear Medicine
Jian Li, Ming Luo, Nanzi Xie, Haifeng Wang, Jianxin Wang
This study aimed to explore the role of certain genes and long non-coding RNAs (lncRNAs) in homocysteine (HCY)-induced vascular endothelial injury. HUVECs were treated with HCY, then cell cycle and apoptosis were analyzed by flow cytometry. HUVECs were then sequenced and analyzed using bioinformatics, with a focus on differentially expressed genes/lncRNA (DEGs/DEL), protein-protein interaction (PPI), functional enrichment analyses, and lncRNA-target prediction. Although HCY did not affect the cell cycle, it significantly increased the number of apoptotic cells...
2018: American Journal of Translational Research
Linping Chen-Wichmann, Marina Shvartsman, Caro Preiss, Colin Hockings, Roland Windisch, Enric Redondo Monte, Georg Leubolt, Karsten Spiekermann, Jörn Lausen, Christian Brendel, Manuel Grez, Philipp A Greif, Christian Wichmann
Chromosomal translocations represent frequent events in leukemia. In t(8;21)+ acute myeloid leukemia, RUNX1 is fused to nearly the entire ETO protein, which contains four conserved nervy homology regions, NHR1-4. Furthermore RUNX1/ETO interacts with ETO-homologous proteins via NHR2, thereby multiplying NHR domain contacts. As shown recently, RUNX1/ETO retains oncogenic activity upon either deletion of the NHR3 + 4 N-CoR/SMRT interaction domain or substitution of the NHR2 tetramer domain. Thus, we aimed to clarify the specificities of the NHR domains...
August 9, 2018: Oncogene
Deborah Ingenhag, Sven Reister, Franziska Auer, Sanil Bhatia, Sarah Wildenhain, Daniel Picard, Marc Remke, Jessica I Hoell, Andreas Kloetgen, Dennis Sohn, Reiner U Jänicke, Gesine Koegler, Arndt Borkhardt, Julia Hauer
The homeobox gene HLXB9 encodes for the transcription factor HB9, which is essential for pancreatic as well as motor neuronal development. Beside its physiologic expression pattern, aberrant HB9 expression has been observed in several neoplasias. Especially in infant translocation t(7;12) acute myeloid leukemia aberrant HB9 expression is the only known molecular hallmark and assumed to be a key factor in leukemic transformation. However, up to now only poor functional data exist addressing the oncogenic potential of HB9 or its influence on hematopoiesis...
August 9, 2018: Haematologica
Dan-Dan Xiong, Yi-Wu Dang, Peng Lin, Dong-Yue Wen, Rong-Quan He, Dian-Zhong Luo, Zhen-Bo Feng, Gang Chen
BACKGROUND: Circular RNAs (circRNAs) have received increasing attention in human tumor research. However, there are still a large number of unknown circRNAs that need to be deciphered. The aim of this study is to unearth novel circRNAs as well as their action mechanisms in hepatocellular carcinoma (HCC). METHODS: A combinative strategy of big data mining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and computational biology was employed to dig HCC-related circRNAs and to explore their potential action mechanisms...
August 9, 2018: Journal of Translational Medicine
Melanie Gentil, Patricia Hugues, Christophe Desterke, Gladys Telliam, Ivan Sloma, Lucas E B Souza, Seda Baykal, Jerome Artus, Frank Griscelli, Agnes Guerci, Hyacinthe Johnson-Ansah, Adlen Foudi, Annelise Bennaceur-Griscelli, Ali G Turhan
Aryl Hydrocarbon Receptor (AHR) is an ubiquitous basic helix-loop-helix transcription factor, which is ligand-activated and involved in numerous biological processes including cell division, cell quiescence and inflammation. It has been shown that AHR is involved in normal hematopoietic progenitor proliferation in human cells. In addition, loss of AHR in knockout mice is accompanied by a myeloproliferative syndrome-like disease, suggesting a role of AHR in hematopoietic stem cell (HSC) maintenance. To study the potential role of AHR pathway in CML progenitors and stem cells, we have first evaluated the expression of AHR in UT-7 cell line expressing BCR-ABL...
2018: PloS One
Carrie B Coleman, Julie Lang, Lydia A Sweet, Nicholas A Smith, Brian M Freed, Zenggang Pan, Bradley Haverkos, Roberta Pelanda, Rosemary Rochford
Epstein Barr virus (EBV) has been classified into two strains, EBV Type-1 (EBV-1) and EBV Type-2 (EBV-2) based on genetic variances and differences in transforming capacity. EBV-1 readily transforms B-cells in culture while EBV-2 is poorly transforming. The differing ability to immortalize B-cells in vitro suggests that in vivo these viruses likely use alternative approaches to establish latency. Indeed, we recently reported that EBV-2 has a unique cell tropism for T-cells, infecting T-cells in culture and in healthy Kenyan infants, strongly suggesting EBV-2 infection of T-cells is a natural part of the EBV-2 life-cycle...
August 8, 2018: Journal of Virology
Michael A Rak, Jason Buehler, Sebastian Zeltzer, Justin Reitsma, Belen Molina, Scott Terhune, Felicia Goodrum
Human cytomegalovirus, HCMV, is a beta-herpesvirus that establishes a life-long latent infection in its host that is marked by recurrent episodes of reactivation. The molecular mechanisms by which the virus and host regulate entry into and exit from latency remain poorly understood. We have previously reported that UL135 is critical for reactivation, functioning in part by overcoming suppressive effects of the latency determinant UL138 We have demonstrated a role for UL135 in diminishing cell surface levels and inhibiting the return of epidermal growth factor receptor (EGFR) to the cell surface...
August 8, 2018: Journal of Virology
Ranran Dai, Youchao Yu, Guofeng Yan, Xiaoxia Hou, Yingmeng Ni, Guochao Shi
BACKGROUND: Adipose-derived mesenchymal stem cell (ASCs) exerts immunomodulatory roles in asthma. However, the underlying mechanism remains unclear. The present study aimed to explore the effects and mechanisms of ASCs on chronic asthma using an ovalbumin (OVA)-sensitized asthmatic mouse model. METHODS: Murine ASCs (mASCs) were isolated from male Balb/c mice and identified by the expression of surface markers using flow cytometry. The OVA-sensitized asthmatic mouse model was established and then animals were treated with the mASCs through intratracheal delivery...
August 8, 2018: BMC Pulmonary Medicine
Stefania Puttini, Isabelle Plaisance, Lucio Barile, Elisabetta Cervio, Giuseppina Milano, Paola Marcato, Thierry Pedrazzini, Giuseppe Vassalli
High aldehyde dehydrogenase (ALDHhi ) activity has been reported in normal and cancer stem cells. We and others have shown previously that human ALDHhi cardiac atrial appendage cells are enriched with stem/progenitor cells. The role of ALDH in these cells is poorly understood but it may come down to the specific ALDH isoform(s) expressed. This study aimed to compare ALDHhi and ALDHlo atrial cells and to identify the isoform(s) that contribute to ALDH activity, and their functional role. Methods and Results: Cells were isolated from atrial appendage specimens from patients with ischemic and/or valvular heart disease undergoing heart surgery...
2018: Frontiers in Cardiovascular Medicine
Ira Sharma, Avninder Singh, Fouzia Siraj, Sunita Saxena
BACKGROUND: Glioblastoma multiforme (GBM) is one of the lethal malignant tumors of the central nervous system. Despite advances made in understanding this complex disease, little has been achieved in improving clinical efficacy towards it. Factors such as chemokines play important role in shaping the tumor microenvironment which in turn plays a significant role in deciding course of tumor progression. In this study, we investigated the role of chemokine IL-8 in glioblastoma progression with particular emphasis on immunomodulation, cellular proliferation, invasion and vascular mimicry...
August 8, 2018: Journal of Biomedical Science
Nicholas Wee Chong Koh, Wan Yi Seow, York Tien Lee, Joyce Ching Mei Lam, Derrick Wen Quan Lian
We report the first case of an ovarian pericytoma with t(7;12). An 11-year-old child presented with abdominal pain and distension. A suprapubic mass was detected on examination and radiological investigations revealed a 16.5 cm solid-cystic ovarian mass. Histologically, the tumor was composed of spindle cells with S100-protein, Bcl-2, and CD10 reactivity on immunohistochemistry. Alpha fetoprotein, calretinin, alpha-inhibin, WT1, smooth muscle actin, caldesmon, desmin, cytokeratins, chromogranin, synaptophysin, EMA, Sox10, CD117, CD31, CD34, and CD68 were all negative...
August 6, 2018: International Journal of Gynecological Pathology
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