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Fanggang Ren, Na Zhang, Zhifang Xu, Jing Xu, Yaofang Zhang, Xiuhua Chen, Yanhong Tan, Jianmei Chang, Hongwei Wang
OBJECTIVE: To investigate the immunophenotypic characteristics of acute promyelocytic leukemia (APL) and explore the sensitivity and specificity of various antibody combinations for the timely and accurate diagnosis APL. METHODS: A retrospective analysis was performed using morphological, immunological, genetic, and molecular biological data from 92 patients diagnosed with APL and 190 controls diagnosed with non-APL acute myeloid leukemia. RESULTS: For APL diagnosis, the CD9/CD11b/human leukocyte antigen (HLA)-DR antibody combination had 85% sensitivity and 95% specificity, AUC = 0...
October 13, 2018: International Journal of Laboratory Hematology
Kathelijne Cjm Kraal, Ilse Timmerman, Hannah M Kansen, Cor van den Bos, Jozsef Zsiros, Henk van den Berg, Sebastiaan F Somers, Eric Braakman, Annemarie M L Peek, Max van Noesel, C Ellen van der Schoot, Marta Fiocco, Huib N Caron, Carlijn Voermans, Godelieve A M Tytgat
PURPOSE: Targeted radiotherapy with 131 Iodine-meta-iodobenzylguanidine (131 I-MIBG) is effective for neuroblastoma (NBL), although optimal scheduling during high-risk (HR) treatment is being investigated. We aimed to evaluate the feasibility of stem cell apheresis and study hematological reconstitution after autologous stem cell transplantation (ASCT) in HR-NBL patients treated with upfront 131 I-MIBG-therapy. EXPERIMENTAL DESIGN: In two prospective multi-center cohort studies, newly diagnosed HR-NBL patients were treated with two courses of 131 I-MIBG-therapy, followed by a HR-induction protocol...
October 12, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Anna Adamska, Aleksandra Araszkiewicz, Stanislaw Pilacinski, Agnieszka Gandecka, Agata Grzelka, Katarzyna Kowalska, Agnieszka Malinska, Michal Nowicki, Dorota Zozulinska-Ziolkiewicz
In patients with diabetes, functional changes in microcirculation and subclinical vascular pathology precede clinical manifestation of microangiopathic complications. The objective of this study was to evaluate the association between established vascular risk factors and density, maturity, and reactivity of dermal blood vessels in adults with type 1 diabetes (DM1). We included 148 DM1 patients (87 men) with a median (IQR) age of 40.5 (30.5-49) years and a median diabetes duration of 21 (17-29.5) years. The control group consisted of 13 healthy volunteers (6 men) with a median (IQR) age of 36 (31-43)...
October 9, 2018: Microvascular Research
XiangHong Li, Wanchang Cui, Lisa Hull, Joan T Smith, Juliann G Kiang, Mang Xiao
In this study, we investigated the effects of low-to-moderate doses of radiation in mice, given our limited understanding of the health risks associated with these exposures. Here, we demonstrate the different responses of the CD2F1 mouse hematopoietic system to low-to-moderate (0.5, 1, 3 or 5 Gy) doses of gamma radiation. After 3 and 5 Gy of 60 Co total-body irradiation (TBI), mouse blood cell counts were decreased and maintained below baseline up to 28-42 days. In contrast, after 0.5 Gy TBI, lymphocyte and monocyte counts increased, and peaked from day 3 to day 14...
October 12, 2018: Radiation Research
Masahito Yamamoto, Jőrg Wilting, Hiroshi Abe, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Shin-Ichi Abe
In and after the third trimester, the lung surface is likely to become smooth to facilitate respiratory movements. However, there are no detailed descriptions as to when and how the lung surface becomes regular. According to our observations of 33 fetuses at 9-16 weeks of gestation (crown-rump length [CRL], 39-125 mm), the lung surface, especially its lateral (costal) surface, was comparatively rough due to rapid branching and outward growing of bronchioli at the pseudoglandular phase of lung development. The pulmonary pleura was thin and, beneath the surface mesothelium, no or little mesenchymal tissue was detectable...
September 2018: Anatomy & Cell Biology
Radoslaw Litwinowicz, Bogusław Kapelak, Jerzy Sadowski, Anna Kędziora, Krzysztof Bartus
Ischemic heart disease is a major cause of death and disabilities worldwide. Unfortunately, not all patients are suitable for direct revascularization. Cell-based therapies may be alternative options because of their potential to promote neovascularisation and endothelial repair, improving myocardial perfusion. The success of cell-based therapies depends on the type of implanted stem cells, delivery method and underlying disease. Several different cell populations including bone marrow-derived mononuclear cells (MNCs), mesenchymal stromal cells (MSCs), CD34+, CD133+, endothelial progenitor cells, adipose-derived mesenchymal stromal cells (ASCs) and stem cells from placenta and umbilical cord have been investigated...
September 2018: Kardiochirurgia i Torakochirurgia Polska, Polish Journal of Cardio-Thoracic Surgery
Marco Giulioni, Gianluca Marucci, Massimo Cossu, Laura Tassi, Manuela Bramerio, Carmen Barba, Anna Maria Buccoliero, Gianfranco Vornetti, Corrado Zenesini, Alessandro Consales, Luca De Palma, Flavio Villani, Giancarlo Di Gennaro, Giampaolo Vatti, Nelia Zamponi, Gabriella Colicchio, Carlo Efisio Marras
OBJECTIVE: To analyze the relationships between CD34 expression and several demographic, clinical and pathological features, in patients with histopathological evidence of low-grade epilepsy-associated tumors (LEATs) who underwent epilepsy surgery. METHODS: We conducted a retrospective study enrolling 187 patients with LEATs who underwent surgery between January 2009 and June 2015 at eight Italian epilepsy surgery centers. All cases were histologically diagnosed according to the WHO classification of tumors of the central nervous system...
October 8, 2018: World Neurosurgery
Farahnaz Noroozinia, Leila Mahmoudzadeh, Farzaneh Hosseini Gharalari, Khadijeh Makhdoomi, Ata Abbasi
OBJECTIVE: Lupus nephritis is one of the most serious and common complications of systemic lupus erythematosus. It has an unpredictable course, and the type, severity, and activity of renal lesions cannot be assessed only by clinical and laboratory findings. The aim of the present study was to determine the relationship between the expression of CD34 and the histopathological findings of lupus nephritis. METHODS: A total of 73 renal biopsy samples of patients with a diagnosis of lupus nephritis were examined for CD34 expression by immunohistochemistry...
October 10, 2018: European Journal of Rheumatology
Marta González-Vicent, Miguel A Díaz Perez
PURPOSE OF REVIEW: Nowadays, T-cell-depleted haploidentical transplantation is considered a valid approach for children lacking a human leukocyte antigen (HLA) identical donor for allogeneic transplantation. This kind of allogeneic transplant is now widely used especially for pediatric patients with high-risk hematological malignancies. However, relapsing disease and life-threatening viral infections are still relevant clinical problems as a consequence of delayed immune reconstitution...
November 2018: Current Opinion in Oncology
Saisha M Muñiz Alers, Kristin Page, Ryan Simmons, Barbara Waters-Pick, Lynn Cheatham, Jesse D Troy, Joanne Kurtzberg
BACKGROUND: The cell dose infused for cord blood transplantation strongly correlates with outcomes following transplantation. Post thaw recoveries can be improved by washing cord blood units (CBUs) in dextran/albumin. Early methods used a labor-intensive manual process. We have recently developed and validated an automated washing method. We now report our results of a study comparing cellular recoveries achieved after manual and automated wash, as well as the impact on engraftment following allogeneic transplantation...
October 11, 2018: Transfusion
Petek Korkusuz, Sevil Köse, Nilgün Yersal, Selin Önen
Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity...
October 11, 2018: Methods in Molecular Biology
Yoshiaki Sonoda
Hematopoietic stem cell (HSC) biology is a current topic of interest having significant implications for clinical HSC transplantation and basic HSC research. It was long believed that the most primitive HSCs in mammals, including those in mice and humans, were CD34 antigen-positive (CD34+ ) cells. However, Nakauchi et al. reported that murine long-term lymphohematopoietic reconstituting HSCs were lineage marker-negative (Lin- ) c-kit+ Sca-1+ CD34-low/negative (CD34low/- ), known as CD34low/- KSL cells. We have previously identified very primitive human cord blood (CB) -derived CD34-negative (CD34- ) severe combined immunodeficiency (SCID) -repopulating cells (SRCs) utilizing an intra-bone marrow injection method and have proposed a new concept that CD34- SRCs (HSCs) reside at the apex of human HSC hierarchy...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Johanna S Ungerstedt
Myeloid hematological malignancies are clonal bone marrow neoplasms, comprising of acute myeloid leukemia (AML), the myelodysplastic syndromes (MDS), chronic myelomonocytic leukemia (CMML), the myeloproliferative neoplasms (MPN) and systemic mastocytosis (SM). The field of epigenetic regulation of normal and malignant hematopoiesis is rapidly growing. In recent years, heterozygous somatic mutations in genes encoding epigenetic regulators have been found in all subtypes of myeloid malignancies, supporting the rationale for treatment with epigenetic modifiers...
October 9, 2018: International Journal of Molecular Sciences
Manas Bajpai, Nilesh Pardhe, Manish Kumar
Spindle cell lipoma (SCL), also called as pleomorphic adenoma, is a rare variant of lipoma histopathologically characterized by an admixture of mature fat cells with spindle cells and occasionally mast cells with myxoid connective tissue stroma and thick bends of birefringent collagen. Although buccal mucosa is the most common location for oral lipomas, for SCL, it is an exceedingly rare location. We report a case of an asymptomatic swelling of buccal mucosa that simulated the features of neurofibroma on histopathological examination, and the final diagnosis of SCL was made on the basis of immunohistochemical features...
October 2018: Indian Journal of Pathology & Microbiology
Yasuko Yamagishi, Takaaki Konuma, Yoriko Miwa, Maki Oiwa-Monna, Susumu Tanoue, Masamichi Isobe, Koji Jimbo, Mai Mizusawa, Hatsuko Narita, Koji Kobayashi, Seiko Kato, Satoshi Takahashi, Arinobu Tojo
Hospital readmissions have been used as a prognostic indicator for patients receiving allogeneic hematopoietic cell transplantation (HCT). However, the impact of readmission during early and mid-phase of cord blood transplantation (CBT) on long-term outcomes has not been fully investigated. We retrospectively analyzed 156 adult patients who received single-unit CBT in our institute. Among this cohort, thirteen patients (8%) were readmitted within 30 days after discharge, and 27 (17%) were readmitted within 90 days after discharge...
October 9, 2018: International Journal of Hematology
Yong Li, Jialin Hu, Hongping Song, Tao Wu
Chronic myeloid leukemia (CML) responds well to BCR-ABL tyrosine kinase inhibitors (TKI), such as imatinib and dasatinib. However, these inhibitors have been less effective as single agents in the blast phase-CML. In this work, we show that anisomycin, a clinically available drug, targets CML cells at all stages of development and enhances BCR-ABL TKIs' efficacy. Anisomycin at nanomolar concentration inhibits proliferation and induces apoptosis in a panel of CML cell lines in a dose-dependent manner. It induces apoptosis CD34 stem/progenitor cells isolated from patients with blast phase CML...
October 6, 2018: Biochemical and Biophysical Research Communications
Anna Margret Halldorsdottir, Sveinlaug Atladottir, Margret A Thorsteinsdottir, Niels A Arnason, Gudmundur Runarsson, Torbjorn Jonsson, Olafur E Sigurjonsson, Sigrun Reykdal
BACKGROUND: Prior to infusion, cryopreserved autologous peripheral blood stem cell (auto-PBSC) grafts can either be thawed at the bedside or thawed and washed at the laboratory. At our center, manual washing of grafts prior to infusion was discontinued in April 2012 and bedside thawing was implemented. METHODS: This study compares the outcomes of two patient groups who received auto-PBSC either after post-thaw washing (n = 84) or bedside thawing (n = 83). RESULTS: No life-threatening infusion-related side effects were reported in either group...
October 9, 2018: Acta Haematologica
Yong Zhan, Fubo Zhou, Xiaoling Yu, Fei Luo, Fangyi Liu, Ping Liang, Zhigang Cheng, Zhiyu Han, Jie Yu
PURPOSE: Previous studies have reported that parameters of dynamic contrast-enhanced ultrasound (DCE-US) could predict prognosis of hepatocellular carcinoma (HCC) patients after antiangiogenic therapies. In this study, we aimed to investigate the correlation of DCE-US parameters and the prognosis of HCC patients after microwave ablation (MWA). MATERIALS AND METHODS: Between June 2012 and January 2018, a total of 35 HCC patients who received MWA with a curative intent were enrolled...
October 9, 2018: International Journal of Hyperthermia
J García-Piqueras, L Carcaba, J Feito, B García, E Viña, J Suárez-Quintanilla, J Cobo, J A Vega, O García-Suárez
Chondroitin sulfate is a glycosaminoglycan involved in maintaining the morpho-functional properties of the extracellular matrix in peripheral nerves, but its distribution in human sensory corpuscles is unknown despite the role of extracellular matrix in mechanotransduction and axonal guidance. In this study we used immunohistochemistry to analyze the distribution of chondroitin sulfate in human cutaneous Meissner and Pacinian corpuscles. Chondroitin sulfate expression was absent from Meissner corpuscles. In Pacinian corpuscles chondroitin sulfate was found associated to a CD34 positive endoneurial-related layer, interposed between the S100 protein positive inner core cells, and the vimentin positive inner core and outer core-capsule cells...
October 9, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Xuchun He, Jiadi Chen
OBJECTIVE: To explore the genetic and clinical features of patients with acute myeloid leukemia (AML) and near-tetraploidy/tetraploidy (NT/T) karyotype. METHODS: Cytogenetic findings of 1836 cases of primary AML were retrospectively analyzed. Karyotypes of the identified cases were confirmed by fluorescence in situ hybridization (FISH). Clinical data including gender, age, morphology, immunophenotype, genetics, and prognosis were reviewed. RESULTS: Nine male and two female patients with NT/T were identified with a median age of 63 years...
October 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
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