Fabio Lino Gratani, Till Englert, Payal Nashier, Peter Sass, Laura Czech, Niels Neumann, Sofia Doello, Petra Mann, Rudolf Blobelt, Siegfried Alberti, Karl Forchhammer, Gert Bange, Katharina Höfer, Boris Macek
Protein Ser/Thr kinases are posttranslational regulators of key molecular processes in bacteria, such as cell division and antibiotic tolerance. Here, we characterize the E. coli toxin YjjJ (HipH), a putative protein kinase annotated as a member of the family of HipA-like Ser/Thr kinases, which are involved in antibiotic tolerance. Using SILAC-based phosphoproteomics we provide experimental evidence that YjjJ is a Ser/Thr protein kinase and its primary protein substrates are the ribosomal protein RpmE (L31) and the carbon storage regulator CsrA...
December 20, 2022: MSystems