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https://www.readbyqxmd.com/read/30509239/combining-high-throughput-imaging-flow-cytometry-and-deep-learning-for-efficient-species-and-life-cycle-stage-identification-of-phytoplankton
#1
Susanne Dunker, David Boho, Jana Wäldchen, Patrick Mäder
BACKGROUND: Phytoplankton species identification and counting is a crucial step of water quality assessment. Especially drinking water reservoirs, bathing and ballast water need to be regularly monitored for harmful species. In times of multiple environmental threats like eutrophication, climate warming and introduction of invasive species more intensive monitoring would be helpful to develop adequate measures. However, traditional methods such as microscopic counting by experts or high throughput flow cytometry based on scattering and fluorescence signals are either too time-consuming or inaccurate for species identification tasks...
December 3, 2018: BMC Ecology
https://www.readbyqxmd.com/read/30506671/high-tumour-plasma-cell-infiltration-reflects-an-important-microenvironmental-component-in-classic-hodgkin-lymphoma-linked-to-presence-of-b-symptoms
#2
Alex R Gholiha, Peter Hollander, Gustaf Hedstrom, Christer Sundstrom, Daniel Molin, Karin E Smedby, Henrik Hjalgrim, Ingrid Glimelius, Rose-Marie Amini, Gunilla Enblad
Plasma cells are important prognostic actors in different malignancies. The tumour microenvironmental composition in classic Hodgkin lymphoma (cHL) is a major prognostic key element; however, clinicopathological studies regarding plasma cells in cHL are lacking. The aim of this study was to investigate CD138+ (also termed SDC1+) plasma cell and IgG4 producing (IgG4+) plasma cells infiltration in the microenvironment of cHL. Immunohistochemistry with anti-CD138 and IgG4 antibodies was performed on diagnostic tumour biopsies from 124 patients with cHL, on tissue micro array (TMA)...
December 2, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/30505187/mitigation-of-adverse-effects-of-heat-stress-on-vicia-faba-by-exogenous-application-of-magnesium
#3
Manzer H Siddiqui, Saud A Alamri, Mutahhar Y Y Al-Khaishany, Mohammed A Al-Qutami, Hayssam M Ali, Mohamed H Al-Whaibi, Mona S Al-Wahibi, Hesham F Alharby
The effects of magnesium (Mg) supplementation on the growth performance, oxidative damage, DNA damage, and photosynthetic pigment synthesis, as well as on the activity level of carbonic anhydrase (CA), ribulose-1,5-bisphosphate carboxylase (Rubisco), and antioxidant enzymes were studied in Vicia faba L. plants exposed to heat stress (HS) and non-heat-stress (non-HS) conditions. Seeds were grown in pots containing a 1:1 mixture of sand and peat, with Mg treatments. The treatments consisted of (i) 0 Mg and non-HS (ambient temperature; control); (ii) 50 mM Mg; (iii) HS (38 °C); and (iv) 50 mM Mg and HS (38 °C)...
November 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/30505179/management-of-the-noxious-weed-medicago-polymorpha-l-via-allelopathy-of-some-medicinal-plants-from-taif-region-saudi-arabia
#4
Mardi Mohammed Algandaby, Salama M El-Darier
Germination bioassay was carried out to test the biological activity of Achillea santolina L. (ASAE), Artemisia monosperma Del. (AMAE), Pituranthus tortuosus L. (PTAE) and Thymus capitatus L. (TCAE) aqueous extracts (collected from Taif region, KSA) on germination percentage (GP), plumule (PL) and radicle (RL) lengths (mm) besides seedling dry weight (SDW) (mg/seedlings) of Medicago polymorpha L. The inhibitory effect of P . tortuosus was insignificant compared to the other three donor species which attained the strongest allelopathic potential in the following order: A ...
November 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/30502172/physico-chemical-chlorophyll-a-species-in-aqueous-alcohol-solutions-determine-the-rate-of-its-discoloration-under-uv-light
#5
Midori Yasuda, Keisuke Oda, Toshihisa Ueda, Masaaki Tabata
Chlorophyll-a (Chl-a) discolors when it is exposed to light, and such discoloration decreases food quality. To elucidate the discoloration mechanism of Chl-a, we determined discoloration rate in different Chl-a chemical species and assessed the size of Chl-a aggregates in mixed aqueous solutions of methanol and ethanol. Chl-a existed as monomer, J-aggregate, and random aggregate in solutions with different alcohol concentrations. The predominant species depended on the alcohol concentration. Monomeric Chl-a and J-aggregates discolored quickly, whereas random aggregates discolored slowly...
March 30, 2019: Food Chemistry
https://www.readbyqxmd.com/read/30501432/inferior-outcomes-and-treatment-disparities-in-elderly-patients-with-classical-hodgkin-lymphoma-a-national-cancer-data-base-analysis
#6
Ajay Major, Matthew W Jackson, Derek E Smith, Manali Kamdar, Rachel Rabinovitch
Elderly patients with classical Hodgkin lymphoma (cHL) are understudied and poorly defined. The National Cancer Data Base was queried for adults with cHL diagnosed 2004-2013: 22,547 age 18-39, 12,841 age 40-59, and 10,873 age ≥60 were identified. Two-year overall survival (OS) was 97%, 91%, and 65% for the three age cohorts, respectively (p < .0001). Elderly patients age ≥60 had greater advanced comorbidity scores, stage III-IV disease, and lymphocyte-depleted histology. Elderly patients were treated less with chemotherapy, radiotherapy for stage I-II disease, and at academic/research centers (p < ...
December 3, 2018: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/30500607/mesohaline-conditions-represent-the-threshold-for-oxidative-stress-cell-death-and-toxin-release-in-the-cyanobacterium-microcystis-aeruginosa
#7
Cliff Ross, B Christopher Warhurst, Amber Brown, Chase Huff, Judith D Ochrietor
As aquatic ecosystems become increasingly affected by hydrologic alterations, drought and sea level rise a need exists to better understand the biological effects of elevated salinity on toxigenic cyanobacteria such as Microcystis aeruginosa. This study investigated the impacts of oligohaline/low mesohaline conditions and exposure time on selected physiological and biochemical responses in M. aeruginosa including cell viability, oxidative stress, antioxidant responses, in addition to microcystin synthesis and release into the surrounding environment...
November 22, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/30496998/toxicity-effects-of-p-choroaniline-on-the-growth-photosynthesis-respiration-capacity-and-antioxidant-enzyme-activities-of-a-diatom-phaeodactylum-tricornutu
#8
Xiufen Wang, Jingjing Miao, Luqing Pan, Yun Li, Yufei Lin, Jiangyue Wu
The environmental risk issues of p-choroaniline have been concerned by the widespread application and transportation of this important chemical intermediate. The information about the toxicity of p-chloroaniline was mainly concentrated on freshwater organisms while the current knowledge on marine organisms was scarce yet. In this study, acute toxicity and toxic physiology characteristic of p-chloroaniline to Phaeodactylum tricornutum (P. tricornutum) were first determined. In the acute experiments, the effect of the p-choroaniline to P...
November 26, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/30482586/nano-metal-oxides-induce-antimicrobial-resistance-via-radical-mediated-mutagenesis
#9
Ye Zhang, April Z Gu, Shanshan Xie, Xiangyang Li, Tianyu Cen, Dan Li, Jianmin Chen
The widespread use of nanoparticles has triggered increasing concern and interest due to the adverse effects on global public health and environmental safety. Whether the presence of nano-metal oxides (NMOs) could facilitate the formation of new antimicrobial resistance genes (ARGs) via de novo mutation is largely unknown. Here, we proved that two widely used NMOs could significantly improve the mutation frequencies of CIP- and CHL-resistant E. coli isolates; however, the corresponding metal ions have weaker effects...
October 25, 2018: Environment International
https://www.readbyqxmd.com/read/30478711/time-resolved-fluorescence-measurements-on-leaves-principles-and-recent-developments
#10
Volha U Chukhutsina, Alfred R Holzwarth, Roberta Croce
Photosynthesis starts when a pigment in the photosynthetic antennae absorbs a photon. The electronic excitation energy is then transferred through the network of light-harvesting pigments to special chlorophyll (Chl) molecules in the reaction centres, where electron transfer is initiated. Energy transfer and primary electron transfer processes take place on timescales ranging from femtoseconds to nanoseconds, and can be monitored in real time via time-resolved fluorescence spectroscopy. This method is widely used for measurements on unicellular photosynthetic organisms, isolated photosynthetic membranes, and individual complexes...
November 26, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/30478709/the-effect-of-different-light-regimes-on-pigments-in-coscinodiscus-granii
#11
Yanyan Su
The influence of six different light regimes throughout the photosynthetically active radiation range (from 400 to 700 nm, including blue, green, yellow, red-orange, red, and white) at two intensities (100 and 300 µmol photons m-2  s-1 ) on pigmentation was assessed for the centric marine diatom Coscinodiscus granii for the first time. Chlorophyll (Chl) a and fucoxanthin were the dominating pigments in all treatments. The cellular concentrations of light harvesting pigment (Chl a, Chl c1 + c2, and fucoxanthin) were higher at 100 than at 300 µmol photons m-2  s-1 at all wavelengths, with the largest increases at red and blue light...
November 26, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/30471592/using-the-multidimensional-synthesis-methods-with-non-parameter-test-multiple-time-scales-analysis-to-assess-water-quality-trend-and-its-characteristics-over-the-past-25-years-in-the-fuxian-lake-china
#12
Junxu Chen, Yan Lyu, Zhifang Zhao, Hong Liu, Hongling Zhao, Zichen Li
Fuxian Lake is one of the most important conservation areas in the southwest of China. Understanding the water-quality trend and characteristics is the basis of area protection for Fuxian Lake. This study combines of the Mann-Kendall, Mann-Kendall-Sneyers, water pollution index (WPI), the trophic state index and time series analysis to detect and characterize the water quality with a set of indicators: transparency (SD), permanganate index (IMn), total phosphorus (TP), total nitrogen (TN), biochemical oxygen demand (BOD5 ), ammonia nitrogen (NH3 -N) and chlorophyll-a (Chl...
November 10, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30471153/mg-dechelatase-is-involved-in-the-formation-of-photosystem-ii-but-not-in-chlorophyll-degradation-in-chlamydomonas-reinhardtii
#13
Ying Chen, Yousuke Shimoda, Makio Yokono, Hisashi Ito, Ayumi Tanaka
The STAY-GREEN (SGR) gene encodes a Mg-dechelatase that catalyzes the conversion of chlorophyll (Chl) a to pheophytin (Pheo) a. This reaction is the first and most important regulatory step in the chlorophyll degradation pathway. Conversely, Pheo a is an indispensable molecule in photosystem (PS) II, suggesting the involvement of SGR in the formation of PSII. To investigate the physiological functions of SGR, we isolated Chlamydomonas sgr mutants by screening an insertion-mutant library. The sgr mutants had reduced maximum quantum efficiency of PSII (Fv/Fm) and reduced Pheo a levels...
November 24, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/30468692/light-capture-and-pigment-diversity-in-marine-and-freshwater-cryptophytes
#14
Brady R Cunningham, Matthew J Greenwold, Eric M Lachenmyer, Kristin M Heidenreich, Abigail C Davis, Jeffry L Dudycha, Tammi L Richardson
Phenotypic traits associated with light capture and phylogenetic relationships were characterized in 34 strains of diversely pigmented marine and freshwater cryptophytes. Nuclear SSU and partial LSU rDNA sequence data from 33 of these strains plus an additional 66 strains produced a concatenated rooted maximum likelihood tree that classified the strains into 7 distinct clades. Molecular and phenotypic data together support: 1) the reclassification of Cryptomonas irregularis NIES 698 to the genus Rhodomonas, 2) revision of phycobiliprotein (PBP) diversity within the genus Hemiselmis to include cryptophyte phycocyanin (Cr-PC) 569, 3) the inclusion of previously unidentified strain CCMP 2293 into the genus Falcomonas, even though it contains cryptophyte phycoerythrin 545 (Cr-PE 545), and 4) the inclusion of previously unidentified strain CCMP 3175, which contains Cr-PE 545, in a clade with PC-containing Chroomonas species...
November 23, 2018: Journal of Phycology
https://www.readbyqxmd.com/read/30466903/prevalence-serotypes-and-drug-resistance-of-nontyphoidal-salmonella-among-paediatric-patients-in-a-tertiary-hospital-in-guangzhou-china-2014-2016
#15
Bingshao Liang, Yongqiang Xie, Shujun He, Jialiang Mai, Yanmei Huang, Liyuan Yang, Huamin Zhong, Qiulian Deng, Shuwen Yao, Yan Long, Yiyu Yang, Sitang Gong, Zhenwen Zhou
PURPOSE: Nontyphoidal Salmonella (NTS) is a common pathogen responsible for acute gastroenteritis among all ages; however, information on the prevalence, serotypes, and antibiotic susceptibility of NTS isolates is limited. We aimed to explore the characteristics of NTS isolated from paediatric patients in Guangzhou, China. METHODS: This was a retrospective study of 4586 stool culture collected at Guangzhou Women and Children's Medical Center from 2014 to 2016. RESULTS: We identified 220 (4...
November 19, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/30450143/correlations-between-capsular-changes-and-rom-restriction-in-frozen-shoulder-evaluated-by-plain-mri-and-mr-arthrography
#16
Kenji Kanazawa, Yoshihiro Hagiwara, Takuya Sekiguchi, Kazuaki Suzuki, Masashi Koide, Akira Ando, Yutaka Yabe
Background: Evaluation of the Range Of Motion (ROM) is one of the important procedures for shoulder disorders. The purpose of this study was to investigate correlations between capsular changes and ROM restrictions evaluated by both plain magnetic resonance imaging (MRI) and Magnetic Resonance Arthrography (MRA) in the same patients with frozen shoulder. Methods: Between March 2015 and June 2016, 24 patients with frozen shoulders (13 male and 11 female patients, mean age 60...
2018: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/30444359/theoretical-model-of-exciton-states-and-ultrafast-energy-transfer-in-heliobacterial-type-i-homodimeric-reaction-center
#17
Akihiro Kimura, Shigeru Itoh
A simple theoretical model of exciton dynamics was proposed to interpret the fast excitation energy-transfer process in the type I homodimeric reaction center of Heliobacterium modesticaldum (hRC); this structure was recently identified and shown to resemble that of the plant/cyanobacterial photosystem I (PSI) reaction center. The exciton state model, which mainly relies on the geometries of 54 bacteriochlorophyll (BChl) g, 4 BChl- g', and 2 chlorophyll (Chl) a on hRC and assumes constant site energy values for the pigments, reproduced the absorption spectrum of hRC rather well...
November 29, 2018: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/30439689/aquatic-ecotoxicity-of-an-antidepressant-sertraline-hydrochloride-on-microbial-communities
#18
Zhaopeng Yang, Tao Lu, Youchao Zhu, Qi Zhang, Zhigao Zhou, Xiangliang Pan, Haifeng Qian
Sertraline hydrochloride (Ser-HCl), a widely used antidepressant, becomes an aquatic contaminant via metabolic excretion and improper disposal; however, it is unknown how Ser-HCl affects aquatic microbial communities. The present study investigated the effects of Ser on the structures of aquatic microbial communities via high-throughput sequencing analyses. Ser-HCl treatment inhibited the growth of two model algae (the green alga, Chlorella vulgaris, and the cyanobacterium, Microcystis aeruginosa) and decreased the chlorophyll a (Chl-a) concentration in the microcosm to reduce the photosynthetic efficiency...
November 12, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30428304/redox-state-dependent-blinking-of-single-photosystem-i-trimers-at-around-liquid-nitrogen-temperature
#19
Sankar Jana, Ting Du, Ryo Nagao, Takumi Noguchi, Yutaka Shibata
Efficient light harvesting in a photosynthetic antenna system is disturbed by a ragged and fluctuating energy landscape of the antenna pigments in response to the conformation dynamics of the protein. This situation is especially pronounced in Photosystem I (PSI) containing red shifted chlorophylls (red Chls) with the excitation energy much lower than the primary donor. The present study was conducted to clarify light-harvesting dynamics of PSI isolated from Synechocystis sp. PCC6803 by using single-molecule spectroscopy at liquid‑nitrogen temperatures...
November 11, 2018: Biochimica et biophysica acta. Bioenergetics
https://www.readbyqxmd.com/read/30426471/circulating-dendritic-cells-deficiencies-as-a-new-biomarker-in-classical-hodgkin-lymphoma
#20
Domenico Galati, Serena Zanotta, Gaetano Corazzelli, Dario Bruzzese, Gaetana Capobianco, Emanuela Morelli, Manuela Arcamone, Rosaria De Filippi, Antonio Pinto
No robust biomarkers have been yet validated to identify the recurrence of disease in classical Hodgkin Lymphoma (cHL) patients upon induction treatment. The relevance of the inflammatory microenvironment in cHL prompted us to investigate the key immunomodulator myeloid dendritic cells type-1 (mDC1), type-2 (mDC2) and plasmacytoid dendritic cells (pDC). Blood DC levels were assessed in 52 newly diagnosed patients through multiparametric flow-cytometry. All but two patients received ABVD regimen (doxorubicin, bleomycin, vinblastine, dacarbazine)...
November 13, 2018: British Journal of Haematology
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