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Mitochondrial validation

Adam J Białas, Paweł P Liberski, Anna Zielińska, Anna Kumor-Kisielewska, Karolina Szewczyk, Joanna Miłkowska-Dymanowska, Przemysław Sitarek, Wojciech J Piotrowski, Paweł Górski
Introduction: Exacerbations of chronic obstructive pulmonary disease (ECOPD) are important events in the course of the disease, negatively influencing health status and disease progression. Therefore, there is a strong need for deeper understanding of the pathology of ECOPD to elaborate new therapeutic approaches and ameliorate prognoses. Contributions of mitochondria to pathobiology of COPD are still under investigation, although growing evidence suggests their important role in this disease...
2018: International Journal of Chronic Obstructive Pulmonary Disease
Yamila P Cardoso, Juan J Rosso, Ezequiel Mabragaña, Mariano González-Castro, Matías Delpiani, Esteban Avigliano, Sergio Bogan, Raphael Covain, Nahuel F Schenone, Juan M Díaz de Astarloa
With an estimate of around 9,000 species, the Neotropical region hosts the greatest diversity of freshwater fishes of the world. Genetic surveys have the potential to unravel isolated and unique lineages and may result in the identification of undescribed species, accelerating the cataloguing of extant biodiversity. In this paper, molecular diversity within the valuable and widespread Neotropical genus Hoplias was assessed by means of DNA Barcoding. The geographic coverage spanned 40 degrees of latitude from French Guiana to Argentina...
2018: PloS One
Toshihiro Tokiwa, Ai Ohnuki, Rie Kubota, Kenichi Tamukai, Kazunori Ike
In this study, we provide the first description of Cystoisospora infection in Asian small-clawed otters ( Aonyx cinereus ). In July 2017, three juvenile otters recently imported from the Republic of Indonesia showed severe diarrhea and were diagnosed with coccidial infection; two of them eventually died. Fecal examination revealed the presence of numerous oocysts. Sporulated oocysts showed typical Cystoisospora features, measuring 24.6 ± 1.6 (22.0-27.0) × 21.8 ± 1.4 (19.0-25.0) μm, with an oocyst length/width ratio of 1...
December 2018: International Journal for Parasitology. Parasites and Wildlife
David G Lassiter, Rasmus J O Sjögren, Brendan M Gabriel, Anna Krook, Juleen R Zierath
OBJECTIVE: We sought to identify AMPK-regulated genes via bioinformatic analysis of microarray data generated from skeletal muscle of animal models with genetically altered AMPK activity. We hypothesized that such genes would play a role in metabolism. Ganglioside-induced differentiation-associated protein 1 (GDAP1), a gene which plays a role in mitochondrial fission and peroxisomal function in neuronal cells but whose function in skeletal muscle is undescribed, was identified and further validated...
July 25, 2018: Molecular Metabolism
A Arun, M I Ansari, P Popli, S Jaiswal, A K Mishra, A Dwivedi, K Hajela, R Konwar
OBJECTIVES: In our ongoing studies to develop ER targeting agents, we screened for dual-acting molecules with a hypothesis that a single molecule can also target both ER positive and negative groups of breast cancer. MATERIALS AND METHODS: 1-(2-(4-(Dibenzo[b,f]thiepin-10-yl)phenoxy)ethyl)piperidine (DTPEP) was synthesized and screened in both MCF-7 (ER+ve) and MDA-MB-231 (ER-ve) cells. Assays for analysis of cell cycle, ROS, apoptosis and MMP loss were carried out using flow cytometry...
August 9, 2018: Cell Proliferation
Sara Fernández, Saúl Rodríguez, Jose L Martínez, Yaisel J Borrell, Alba Ardura, Eva García-Vázquez
Rivers are a vital resource for human wellbeing. To reduce human impact on water bodies, the European Union has established an essential regulatory framework for protection and sustainable management (WFD; 2000/60/EC). In this strategy, reliable and economic bioindicators are a fundamental component. Benthic macroinvertebrates are the group most commonly used as bioindicators through all European countries. However, their conventional assessment currently entails serious cost-efficiency limitations. In this study, we have tested the reliability of metabarcoding as a tool to record river macroinvertebrates using samples from a mock community (in vitro validation) and eDNA extracted for field validation from water from six sites within a north Iberian river (River Nalón, Asturias, Spain)...
2018: PloS One
Prateek Sharma, Sanjeev Kumar
PURPOSE: Despite a growing body of evidence indicating a potential efficacy of the anti-diabetic metformin as anti-cancer agent, the exact mechanism underlying this efficacy has remained largely unknown. Here, we aimed at assessing putative mechanisms associated with the ability of metformin to reduce the proliferation and migration of breast cancer cells. METHODS: A battery of in vitro assays including MTT, colony formation, NBT and scratch wound healing assays were performed to assess the viability, proliferation, anti-oxidative potential and migration of breast cancer-derived MCF-7, MDA-MB-231 and T47D cells, respectively...
August 7, 2018: Cellular Oncology (Dordrecht)
Yongfeng Yang, Lu Wang, Lingyi Li, Wenqiang Li, Yan Zhang, Hong Chang, Xiao Xiao, Ming Li, Luxian Lv
Previous genome-wide association studies (GWAS) suggest that rs10489202 in the intron of MPC2 (mitochondrial pyruvate carrier 2) is a risk locus for schizophrenia in Han Chinese populations. To validate this discovery, we conducted a replication analysis in an independent case-control sample of Han Chinese ancestry (437 cases and 2031 controls), followed by a meta-analytic investigation in multiple East Asian samples. In the replication analysis, rs10489202 showed marginal association with schizophrenia (two-tailed P = 0...
August 7, 2018: Translational Psychiatry
Wenna Kong, Mi Zhou, Qing Li, Wenjie Fan, Haixia Lin, Renxiao Wang
The Bcl-2 family proteins are key regulators of the intrinsic apoptotic pathway and are among the validated targets for developing anticancer drugs. Protein-protein interactions between the pro- and antiapoptotic members of this family determine mitochondrial outer-membrane permeabilization. Elucidating such protein-protein interactions in a quantitative way is helpful for network pharmacology studies on the Bcl-2 family, which, in turn, will provide valuable guidance for developing new anticancer therapies...
August 7, 2018: ChemMedChem
Maria R Love, Jirapas Sripetchwandee, Siripong Palee, Siriporn C Chattipakorn, Morton M Mower, Nipon Chattipakorn
Currently, electrical stimulation (ES) is used to induce changes in various tissues and cellular processes, but its effects on mitochondrial dynamics and mechanisms are unknown. The aim of this study was to compare the effects of monophasic and biphasic, anodal, and cathodal ES on apoptosis, proliferation, and mitochondrial dynamics in neuroblastoma SH-SY5Y cells. Cells were cultured and treated with ES. Alamar blue assay was performed to measure cell proliferation. The proteins expression of apoptotic-related proteins Bcl-2 associated X (Bax), B cell lymphoma 2 (Bcl-2), optic-atrophy-1 (OPA1), mitofusin2 (Mfn2), phosphorylated dynamin-related protein 1 at serine 616 (p-DRP1), and total dynamin-related protein 1 (Total-DRP1) were also determined...
August 4, 2018: Journal of Cellular Physiology
Venkata N Sure, Siva S V P Sakamuri, Jared A Sperling, Wesley R Evans, Ivan Merdzo, Ricardo Mostany, Walter L Murfee, David W Busija, Prasad V G Katakam
Cerebral blood flow (CBF) is uniquely regulated by the anatomical design of the cerebral vasculature as well as through neurovascular coupling. The process of directing the CBF to meet the energy demands of neuronal activity is referred to as neurovascular coupling. Microvasculature in the brain constitutes the critical component of the neurovascular coupling. Mitochondria provide the majority of ATP to meet the high-energy demand of the brain. Impairment of mitochondrial function plays a central role in several age-related diseases such as hypertension, ischemic brain injury, Alzheimer's disease, and Parkinson disease...
August 3, 2018: GeroScience
Mohammed Majrashi, Mohammed Almaghrabi, Maali Fadan, Ayaka Fujihashi, Brian Lee, Jack Deruiter, C Randall Clark, Muralikrishnan Dhanasekaran
Designer drugs are synthetically formulated to mimic the psychostimulatory effects of an original controlled/illegal drug of abuse. Designer drugs have similar chemical structure or functional analog as compared to existing controlled psychostimulatory drugs. There is a substantial rise in the production and use of designer drugs globally. Piperazine designer drugs were synthesized as an alternative to MDMA and have shown to induce numerous toxic effects leading to huge health, safety, law enforcement & monetary problems, and lethality...
July 29, 2018: Life Sciences
Li Cheng, Lin Li, Liling Wang, Xiaofang Li, Hui Xing, Jinting Zhou
Ovarian cancer (OC) is associated with a poor prognosis due to difficulties in early detection. The aims of the present study were to construct a recurrence risk prediction model and to reveal important OC genes or pathways. RNA sequencing data was obtained for 307 OC samples, and the corresponding clinical data were downloaded from The Cancer Genome Atlas database. Additionally, two validation datasets, GSE44104 (20 recurrent and 40 non‑recurrent OC samples) and GSE49997 (204 OC samples), were obtained from the Gene Expression Omnibus database...
July 19, 2018: Molecular Medicine Reports
Michael D Roberts, Matthew A Romero, Christopher B Mobley, Petey W Mumford, Paul A Roberson, Cody T Haun, Christopher G Vann, Shelby C Osburn, Hudson H Holmes, Rory A Greer, Christopher M Lockwood, Hailey A Parry, Andreas N Kavazis
Background: We sought to examine how 12 weeks of resistance exercise training (RET) affected skeletal muscle myofibrillar and sarcoplasmic protein levels along with markers of mitochondrial physiology in high versus low anabolic responders. Methods: Untrained college-aged males were classified as anabolic responders in the top 25th percentile (high-response cluster (HI); n = 13, dual x-ray absorptiometry total body muscle mass change (Δ) = +3.1 ± 0.3 kg, Δ vastus lateralis (VL) thickness = +0...
2018: PeerJ
Giampaolo Morciano, Delia Preti, Gaia Pedriali, Giorgio Aquila, Sonia Missiroli, Anna Fantinati, Natascia Caroccia, Salvatore Pacifico, Massimo Bonora, Anna Talarico, Claudia Morganti, Paola Rizzo, Roberto Ferrari, Mariusz Roman Wieckowski, Gianluca Campo, Carlotta Giorgi, Claudio Trapella, Paolo Pinton
Recent cardiology research studies have reported the role, function and structure of the mitochondrial permeability transition pore (mPTP) and have shown that its opening plays a key role in the progression of myocardial cell death secondary to reperfusion. In this manuscript, we validated a new pharmacological approach as an adjunct to reperfusion in myocardial infarction (MI) treatment and describe the discovery, optimization and structure-activity relationship (SAR) studies of the first small-molecule mPTP opening inhibitors based on a 1,3,8-triazaspiro[4...
July 30, 2018: Journal of Medicinal Chemistry
Jessica Lee, Zachary Pappalardo, Deeksha Gambhir Chopra, Thomas G Hennings, Ian Vaughn, Christopher Lan, Justin J Choe, Kenny Ang, Steven Chen, Michelle Arkin, Michael T McManus, Michael S German, Gregory M Ku
Insulin production by the pancreatic beta cell is critical for the glucose homeostasis of the whole organism. While transcription factors required for insulin production are known, the upstream pathways that control insulin production are less clear. To further elucidate this regulatory network, we created a genetic interaction map of insulin production by performing ∼20,000 pairwise RNA interference (RNAi) knockdowns of insulin promoter regulators. Our map correctly predicted known physical complexes in the electron transport chain as well as a role for Spry2 in the unfolded protein response...
July 27, 2018: Endocrinology
August E Woerner, Angie Ambers, Frank R Wendt, Jonathan L King, Rodrigo Soares Moura-Neto, Rosane Silva, Bruce Budowle
Sequencing whole mitochondrial genomes by capillary electrophoresis is a costly and time/labor-intensive endeavor. Many of the previous Sanger sequencing-based approaches generated amplicons that were several kilobases in length; lengths that are likely not amenable for most forensic applications. However, with the advent of massively parallel sequencing (MPS) short-amplicon multiplexes covering the entire mitochondrial genome can be sequenced relatively easily and rapidly. Recently, the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific by Applied Biosystems™) has been introduced...
July 18, 2018: Forensic Science International. Genetics
Lyudmila Ounpuu, Laura Truu, Igor Shevchuk, Vladimir Chekulayev, Aleksandr Klepinin, Andre Koit, Kersti Tepp, Marju Puurand, Egle Rebane-Klemm, Tuuli Käämbre
The aim of this work was to explore the key bioenergetic properties attributed to the mitochondrial respiration in widely used Caco-2 cell line and human colorectal cancer (HCC) postoperational tissue samples. Oxygraphy and Metabolic Control Analysis (MCA) were applied to estimate the function of oxidative phosphorylation in cultured Caco-2 cells and HCC tissue samples. The mitochondria of Caco-2 cells and HCC tissues displayed larger functional activity of respiratory complex (C)II compared to CI, whereas in normal colon tissue an inverse pattern in the ratio of CI to CII activity was observed...
July 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Pan Liu, Xiao Chen, Haizhe Zhou, Liqun Wang, Zaijun Zhang, Xiaohu Ren, Feiqi Zhu, Yi Guo, Xinfeng Huang, Jianjun Liu, Peter S Spencer, Xifei Yang
Alzheimer's disease (AD), the most common neurodegenerative disease, has no effective treatment. Dauricine (DAU), a benzyl tetrahydroisoquinoline alkaloid isolated from the root of Menispermum dauricum DC, reportedly has neuroprotective effects in cerebral ischemia. Here, we investigated the effects of DAU on N2a cells stably transfected with Swedish mutant amyloid precursor protein (N2a/APP), an AD-like cell model. ELISA and Western blot analysis revealed that DAU inhibited APP processing and reduced A β accumulation...
2018: Oxidative Medicine and Cellular Longevity
Jean-Luc Gattolliat, Boris C Kondratieff, Thomas Kaltenbach, Hathal M Al Dhafer
Mayfly larvae and imagos were collected at approximately 50 localities of the Kingdom of Saudi Arabia (KSU). Included in this material, three species of Labiobaetis Novikova & Kluge, 1987 are recorded, two of them being new to science. Labiobaetis potamoticus Gattolliat & Al Dhafer, sp. n. is described from both larvae and adults, whereas Labiobaetis alahmadii Gattolliat & Al Dhafer, sp. n. is only known from the larval stage. The two species are compared morphologically with Palearctic and Afrotropical species of Labiobaetis ...
2018: ZooKeys
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