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Mobilized Stem Cell

Georgine E de Greef, Eric Braakman, Bronno van der Holt, Jeroen J W M Janssen, Eefke Petersen, Vladimir Vucinic, Nicole Thuss, Meriam Grootes, Jan J Cornelissen
BACKGROUND: Plerixafor (PFX) mobilizes CD34+ cells into circulation by disrupting the CXCR4 binding of the hematopoietic stem cell in its bone marrow niche. STUDY DESIGN AND METHODS: in the prospective HOVON-107 study ( 23 allogeneic HLA-identical sibling donors received one or two subcutaneous (sc) injections of plerixafor 0.320 mg/kg.The primary endpoint, was defined as feasibility to mobilize a minimum of 2.0 x106 CD34+ cells/kg recipient weight obtained by leukopheresis in at least 90% of the donors...
December 11, 2018: Transfusion
Kingsley O Asiedu, Munira Ferdousi, Phuongnga T Ton, Stephen S Adler, Peter L Choyke, Noriko Sato
BACKGROUND: Bone fracture healing is dependent upon the rapid migration and engraftment of bone marrow (BM) progenitor and stem cells to the site of injury. Stromal cell-derived factor-1 plays a crucial role in recruiting BM cells expressing its receptor CXCR4. Recently, a CXCR4 antagonist, plerixafor, has been used to mobilize BM cells into the blood in efforts to enhance cell migration to sites of injury presumably improving healing. In this study, we employed zirconium-89 (89 Zr)-oxine-labeled BM cells imaged with positron emission tomography (PET)/computed tomography (CT) to visualize and quantitate BM cell trafficking following acute bone injury and to investigate the effect of plerixafor on BM cell homing...
December 13, 2018: EJNMMI Research
Mateusz Adamiak, Ahmed Abdel-Latif, Mariusz Z Ratajczak
No abstract text is available yet for this article.
November 16, 2018: Oncotarget
Heuijoon Park, Sonali Lad, Kelsey Boland, Kelly Johnson, Nyssa Readio, Guangchun Jin, Samuel Asfaha, Kelly S Patterson, Ashok Singh, Xiangdong Yang, Douglas Londono, Anupama Singh, Carol Trempus, Derek Gordon, Timothy C Wang, Rebecca J Morris
We used allogeneic bone marrow transplantation (BMT) and a mouse multistage cutaneous carcinogenesis model to probe recruitment of bone marrow-derived epithelial cells (BMDECs) in skin tumors initiated with the carcinogen, dimethylbenz[a]anthracene (DMBA), and promoted with 12-O-tetradecanolyphorbol-13-acetate (TPA). BMDECs clustered in the lesional epithelium, expressed cytokeratins, proliferated, and stratified. We detected cytokeratin induction in plastic-adherent bone marrow cells (BMCs) cultured in the presence of filter-separated keratinocytes (KCs) and bone morphogenetic protein 5 (BMP5)...
December 13, 2018: Nature Communications
Weijie Xie, Ping Zhou, Yifan Sun, Xiangbao Meng, Ziru Dai, Guibo Sun, Xiaobo Sun
Cerebral ischemia-reperfusion is a complicated pathological process. The injury and cascade reactions caused by cerebral ischemia and reperfusion are characterized by high mortality, high recurrence, and high disability. However, only a limited number of antithrombotic drugs, such as recombinant tissue plasminogen activator (r-TPA), aspirin, and heparin, are currently available for ischemic stroke, and its safety concerns is inevitable which associated with reperfusion injury and hemorrhage. Therefore, it is necessary to further explore and examine some potential neuroprotective agents with treatment for cerebral ischemia and reperfusion injury to reduce safety concerns caused by antithrombotic drugs in ischemic stroke...
December 12, 2018: Cells
Zidong Jia, Ye Zhang, Qiang Li, Zhenzhen Ye, Yuqi Liu, Changzhu Fu, Xiaohui Cang, Meng Wang, Min-Xin Guan
The tissue specificity of mitochondrial tRNA mutations remains largely elusive. In this study, we demonstrated the deleterious effects of tRNAThr 15927G>A mutation that contributed to pathogenesis of coronary artery disease. The m.15927G>A mutation abolished the highly conserved base-pairing (28C-42G) of anticodon stem of tRNAThr. Using molecular dynamics simulations, we showed that the m.15927G>A mutation caused unstable tRNAThr structure, supported by decreased melting temperature and slower electrophoretic mobility of mutated tRNA...
December 12, 2018: Nucleic Acids Research
A Nonino, J M Nascimento, C C Mascarenhas, J F Mazzeu, R W Pereira, R H Jacomo
Myelofibrosis (MF) is characterized by increased circulating hematopoietic progenitor cells (HPCs), abnormal cytokine levels, and the survival advantage of neoplastic progenitors over their normal counterparts, which leads to progressive disappearance of polyclonal hematopoiesis. CD47 is a surface glycoprotein with many functions, such as acting as a phagocytosis inhibitor of the expressing cell, that is increased in normal hematopoietic stem and progenitor cells mobilized into the blood and several human cancer-initiating cells, such as in acute myeloid leukemia...
December 10, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Siw Johannesen, Bettina Budeus, Sebastian Peters, Sabine Iberl, Anne-Louise Meyer, Tina Kammermaier, Eva Wirkert, Tim-Henrik Bruun, Verena C Samara, Wilhelm Schulte-Mattler, Wolfgang Herr, Armin Schneider, Jochen Grassinger, Ulrich Bogdahn
Objective: To evaluate safety, tolerability and feasibility of long-term treatment with Granulocyte-colony stimulating factor (G-CSF), a well-known hematopoietic stem cell factor, guided by assessment of mobilized bone marrow derived stem cells and cytokines in the serum of patients with amyotrophic lateral sclerosis (ALS) treated on a named patient basis. Methods: 36 ALS patients were treated with subcutaneous injections of G-CSF on a named patient basis and in an outpatient setting. Drug was dosed by individual application schemes (mean 464 Mio IU/month, range 90-2160 Mio IU/month) over a median of 13...
2018: Frontiers in Neurology
Valentina Poletti, Fabrizia Urbinati, Sabine Charrier, Guillaume Corre, Roger P Hollis, Beatriz Campo Fernandez, Samia Martin, Michael Rothe, Axel Schambach, Donald B Kohn, Fulvio Mavilio
Sickle cell disease (SCD) is caused by a mutation (E6V) in the hemoglobin (Hb) β-chain that induces polymerization of Hb tetramers, red blood cell deformation, ischemia, anemia, and multiple organ damage. Gene therapy is a potential alternative to human leukocyte antigen (HLA)-matched allogeneic hematopoietic stem cell transplantation, available to a minority of patients. We developed a lentiviral vector expressing a β-globin carrying three anti-sickling mutations (T87Q, G16D, and E22A) inhibiting axial and lateral contacts in the HbS polymer, under the control of the β-globin promoter and a reduced version of the β-globin locus-control region...
December 14, 2018: Molecular Therapy. Methods & Clinical Development
Caghan Kizil, Prabesh Bhattarai
Alzheimer's disease (AD) is the most common neurodegenerative disease and is the leading form of dementia. AD entails chronic inflammation, impaired synaptic integrity and reduced neurogenesis. The clinical and molecular onsets of the disease do not temporally overlap and the initiation phase of the cellular changes might start with a complex causativeness between chronic inflammation, reduced neural stem cell plasticity and neurogenesis. Although the immune and neuronal aspects in AD are well studied, the neural stem cell-related features are far less investigated...
2018: Frontiers in Cell and Developmental Biology
Naoyuki Uchida, Keiko U Torii
Stem cells are specific cells that renew themselves and also provide daughter cells for organ formation. In plants, primary stem cell populations are nurtured within shoot and root apical meristems (SAM and RAM) for the production of aerial and underground parts, respectively. This review article summarizes recent progress on control of stem cells in the SAM from studies of the model plant Arabidopsis thaliana. To that end, a brief overview of the RAM is provided first to emphasize similarities and differences between the two apical meristems, which would help in better understanding of stem cells in the SAM...
December 6, 2018: Cellular and Molecular Life Sciences: CMLS
Takashi Kido, Shigeru Miyagawa, Takasumi Goto, Katsuto Tamai, Takayoshi Ueno, Koichi Toda, Toru Kuratani, Yoshiki Sawa
OBJECTIVES: We hypothesized that systemic administration of high-mobility group box 1 fragment attenuates the progression of myocardial fibrosis and cardiac dysfunction in a hamster model of dilated cardiomyopathy by recruiting bone marrow mesenchymal stem cells thus causing enhancement of a self-regeneration system. METHODS: Twenty-week-old J2N-k hamsters, which are δ-sarcoglycan-deficient, were treated with systemic injection of high-mobility group box 1 fragment (HMGB1, n = 15) or phosphate buffered saline (control, n = 11)...
2018: PloS One
Mudasir Bashir Gugjoo, Amarpal, Mujeeb R Fazili, Riaz A Shah, Gutulla Taru Sharma
Characteristic features like self-renewal, multilineage differentiation potential, and immune-modulatory/anti-inflammatory properties, besides the ability to mobilize and home distant tissues make stem cells (SCs) a lifeline for an individual. Stem cells (SCs) if could be harvested and expanded without any abnormal change may be utilized as an all-in-one solution to numerous clinical ailments. However, slender understanding of their basic physiological properties, including expression potential, behavioral alternations during culture, and the effect of niche/microenvironment has currently restricted the clinical application of SCs...
December 4, 2018: Journal of Cellular Physiology
Kaya Xu, Jea-Young Lee, Yuji Kaneko, Julian P Tuazon, Fernando Vale, Harry van Loveren, Cesario V Borlongan
Despite mounting evidence of a massive peripheral inflammatory response accompanying stroke, the ability of intracerebrally transplanted cells to migrate to the periphery and sequester systemic inflammation remains unexamined. Here, we tested the hypothesis that human bone marrow mesenchymal stromal cells intracerebrally transplanted in the brain of adult rats subjected to experimental stroke can migrate to the spleen, a vital organ that confers peripheral inflammation after stroke. Adult Sprague-Dawley rats received sham or experimental stroke via the one-hour middle cerebral artery occlusion model...
December 4, 2018: Haematologica
Debra A Bourne, R Dallin Thomas, Jacqueline Bliley, Gretchen Haas, Aaron Wyse, Albert Donnenberg, Vera S Donnenberg, Ian Chow, Rory Cooper, Sydney Coleman, Kacey Marra, Paul F Pasquina, J Peter Rubin
Soft-tissue deficits in amputation stumps can lead to significant pain and disability. An emerging treatment option is stem cell-enriched fat grafting. This is the first study assessing the potential for this treatment modality in lower extremity amputation sites. In this prospective cohort study, five injured military personnel suffering from pain and limited function at amputation sites were recruited. Fat grafting enriched with stromal vascular fraction was performed at amputation sites to provide additional subcutaneous tissue padding over bony structures...
November 2018: Plastic and Reconstructive Surgery
Jaime Gómez-Morales, Cristóbal Verdugo-Escamilla, Raquel Fernández-Penas, Carmen Maria Parra-Milla, Christophe Drouet, Michele Iafisco, Francesca Oltolina, Maria Prat, Jorge Fernando Fernández-Sánchez
Biocompatible nanosystems exhibiting long-lifetime (∼millisecond) luminescence features are particularly relevant in the field of bioimaging. In this study, citrate-functionalized calcium-doped europium phosphates nanophosphors of the rhabdophane type were prepared at different synthesis times by a bioinspired crystallization route, consisting in thermal decomplexing of Ca2+ /Eu3+ /citrate/phosphate/carbonate solutions. The general formula of this material is Caα Eu1-α (PO4 )1-α (HPO4 )α ·nH2 O, with α ranging from 0 to 0...
November 23, 2018: Journal of Colloid and Interface Science
Jinyeong Yu, Hye Min Kim, Kwang Pyo Kim, Youngsook Son, Min-Sik Kim, Ki-Sook Park
Endogenous bone marrow-derived mesenchymal stem cells (BM-MSCs) are mobilized into peripheral blood and injured tissues by various growth factors and cytokines that are expressed in the injured tissues, such as substance P (SP), stromal cell derived factor-1 (SDF-1), and transforming growth factor-beta (TGF-β). Extracellular bioactive lipid metabolites such as ceramide-1-phosphate and sphingosine-1-phosphate also modulate BM-MSC migration as SP, SDF-1, and TGF-β. However, the roles of intrinsic lipid kinases of BM-MSCs in the stem cell migration are unclear...
November 28, 2018: Biochemical and Biophysical Research Communications
Cun-Bang Wang, Hai Bai, Rui Xi, Yao-Zhu Pan, Tao Wu, Shu-Fen Xu, Qian Zhang, Jin-Mao Zhou, Ya Chen
OBJECTIVE: To evaluate the efficacy of autologous peripheral blood hematopoietic stem cell transplantation (auto-PBHSCT) on patients with multiple myeloma( MM) after Sequential different chemotherapy. METHODS: Seven cases of patients with MM were included in the A group, and 14 cases of patients received 4-6 courses of chemotherapy with VAD and MP before transplantation were included in the B group and received 4-6 courses of chemotherapy with VTD and VD before transplantation...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Evangelos Fergadis, Niki Gavrielatou, Nikos Skouteris, Aggelos Athanasopoulos, Evangelos Lianos, Christos Kosmas
Salvage high-dose chemotherapy (HDC) together with autologous hematopoietic stem cell (HSC) transplantation (ASCT) represents a curative treatment option for patients with relapsed/refractory germ-cell tumor. Usually, 2-3 cycles of HDC are administered with encouraging results, and a sizeable percentage of patients experience long-term survival. However, an appreciable number of patients fail to mobilize adequate numbers of HSCs, adequate to support more than one HDC cycle. There have been no data so far on remobilization of HSCs after prior autografting...
November 26, 2018: Anti-cancer Drugs
J Xia, S N Chen, J Chen, Y Fan, F Chen, X Ma, M Miao, D P Wu
Objective: To explore the efficacy and safety of haploidentical hematopoietic stem cell transplantation (Haplo-HSCT) for paroxysmal nocturnal hemoglobinuria (PNH). Methods: A total of 17 patients with PNH who received Haplo-HSCT from January 2013 to September 2017 were analyzed retrospectively. Results: Of them, 4 patients had de novo PNH, 13 patients had aplastic anemia-PNH syndrome (AA-PNH). All patients received modified busulfan and Cytoxan (BuCy)-based regimens combined with anti-thymocyte globulin (ATG)...
November 14, 2018: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
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