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Mobilized Stem Cell

Ali R Ahmadi, Maria Chicco, Russell N Wesson, Robert A Anders, Frank J M F Dor, Jan N M IJzermans, Tyler J Creamer, George M Williams, Zhaoli Sun, Andrew M Cameron
INTRODUCTION: Acute liver failure (ALF) affects 2000 Americans each year with no treatment options other than liver transplantation. We showed previously that mobilization of endogenous stem cells is protective against ALF in rodents. The objective of this study was to assess whether stem cell mobilizing drugs are lifesaving in a large animal preclinical model of ALF, to assess readiness for a clinical trial. METHODS: Male Yorkshire pigs (14-18 kg) were divided into 2 groups, control (n = 6) and treatment (n = 6)...
August 10, 2018: Annals of Surgery
Wan-Tseng Hsu, Cheng-Hsin Lin, Hsiang-Yiang Jui, Ya-Hsuan Tseng, Chia-Tung Shun, Ming-Chu Hsu, Kenneth Kun-Yu Wu, Chii-Ming Lee
BACKGROUND: CXCR4 blockade is pursued as an alternative to mesenchymal stem cell (MSC) treatment in transplantation based on our previous report that burixafor, through CXCR4 antagonism, mobilizes immunomodulatory MSCs. Here, we explored the efficacy of combining mycophenolate mofetil (MMF)-based immunosuppressants with repetitive burixafor administration. METHODS: Swine heterotopic cardiac allograft recipients received MMF and corticosteroids (control, n = 10) combined with burixafor as a 2-dose (burixafor2D, n = 7) or 2-dose plus booster injections (burixafor2D+B, n = 5) regimen...
August 8, 2018: Transplantation
Cindy Varga, Raymond L Comenzo
The application of high-dose melphalan and autologous stem cell transplant (SCT) to systemic AL amyloidosis (AL) has evolved over the past two decades and remains an important component of therapy for patients with AL. The era of novel agents created the opportunity to provide well -tolerated induction and post-SCT consolidation to AL patients eligible for SCT and the current availability of new monoclonal antibody therapies will likely provide additional opportunities to enhance the outcomes with SCT. In this review, we touch on the history of SCT for AL and examine the data on eligibility, mobilization, induction, risk-adapted melphalan dosing, engraftment, consolidation and maintenance, and long-term outcomes with SCT...
August 8, 2018: Bone Marrow Transplantation
Hiroaki Tanaka, Chihiro Kuwabara, Kensuke Kayamori, Ryo Shimizu, Yoshio Suzuki
No abstract text is available yet for this article.
August 8, 2018: Journal of Clinical and Experimental Hematopathology: JCEH
Damianos S Skopelitis, Kristine Hill, Simon Klesen, Cristina F Marco, Patrick von Born, Daniel H Chitwood, Marja C P Timmermans
Mobile small RNAs serve as local positional signals in development and coordinate stress responses across the plant. Despite its central importance, an understanding of how the cell-to-cell movement of small RNAs is governed is lacking. Here, we show that miRNA mobility is precisely regulated through a gating mechanism polarised at defined cell-cell interfaces. This generates directional movement between neighbouring cells that limits long-distance shoot-to-root trafficking, and underpins domain-autonomous behaviours of small RNAs within stem cell niches...
August 6, 2018: Nature Communications
Garyfalia Karponi, Penelope-Georgia Papayanni, Fani Zervou, Asimina Bouinta, Achilles Anagnostopoulos, Evangelia Yannaki
Stable gene marking and effective engraftment of gene-modified CD34+ hematopoietic stem cells is a prerequisite for gene therapy success, but may be challenged by the inevitable cryopreservation of the final product, prior to extensive quality assurance testing. We investigated the β-globin gene transfer potency in fresh and cryopreserved CD34+ cells from mobilized patients with β-thalassemia, as well as the qualitative impact of repeated freeze/thaw cycles on the functionality of cultured and unmanipulated CD34+ cells in terms of engrafting capacity in a xenotransplantation model, under partial myeloablation...
August 4, 2018: Human Gene Therapy Methods
Thomas S Wilhelm, Zihao Wang, Mohadeseh A Baboli, Jian Yan, Stefan F Preble, Parsian K Mohseni
The ternary III-V semiconductor compound, Al x Ga1 -x As, is an important material that serves a central role within a variety of nanoelectronic, optoelectronic, and photovoltaic devices. With all of its uses, the material itself poses a host of fabrication difficulties stemming from conventional top-down processing, including standard wet-chemical etching and reactive-ion etching (RIE). Metal-assisted chemical etching (MacEtch) techniques provide low-cost and benchtop methods that combine many of the advantages of RIE and wet-chemical etching, without being hindered by many of their disadvantages...
August 6, 2018: ACS Applied Materials & Interfaces
Anne Baudry, Benoit Schneider, Jean-Marie Launay, Odile Kellermann
Genetic and pharmacological studies provided evidence that serotonin (5-HT) is an important signaling molecule for the development and the maintenance of mineralized tissues. However, how 5-HT takes part to the homeostasis of teeth and bone remains elusive. In the dental field, a major breakthrough comes from the identification of 5-HT but also dopamine (DA) as "damage" signals necessary for stem cell-based tooth repair. Pulpal stem cells express the overall functions of 5-HT and DA neurons including a definite set of functional 5-HT/DA receptors that render cells responsive for circulating bioamines...
August 2, 2018: Biochimie
Meimei Dong, Dawn P Spelke, Young Kwang Lee, Jean K Chung, Cheng-Han Yu, David V Schaffer, Jay T Groves
Interactions between EphB4 receptor tyrosine kinases and their membrane-bound ephrin-B2 ligands on apposed cells play a regulatory role in neural stem cell differentiation. With both receptor and ligand constrained to move within the membranes of their respective cells, this signaling system inevitably experiences spatial confinement and mechanical forces in conjunction with receptor-ligand binding. In this study, we reconstitute the EphB4-ephrin-B2 juxtacrine signaling geometry using a supported-lipid-bilayer system presenting laterally mobile and monomeric ephrin-B2 ligands to live neural stem cells...
July 23, 2018: Biophysical Journal
Angela Rivers, Ramasamy Jagadeeswaran, Donald Lavelle
Sickle cell disease (SCD) is caused by a mutation of the b-globin gene(21) that triggers the polymerization of deoxygenated sickle hemoglobin (HbS). Approximately 100,000 SCD patients in the U.S. and millions worldwide(53) suffer from chronic hemolytic anemia, painful crises, multisystem organ damage, and reduced life expectancy(60, 70). Hematopoietic stem cell (HSC) transplantation can be curative, but the majority of patients do not have a suitable donor (80). Advanced gene editing technologies also offer the possibility of a cure (16, 34), but the likelihood that these strategies can be mobilized to treat the large numbers of patients residing in developing countries is remote...
August 1, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Takayasu Ohtake, Yasuhiro Mochida, Kunihiro Ishioka, Machiko Oka, Kyoko Maesato, Hidekazu Moriya, Sumi Hidaka, Satoshi Higashide, Tetsuya Ioji, Yasuyuki Fujita, Atsuhiko Kawamoto, Masanori Fukushima, Shuzo Kobayashi
Critical limb ischemia (CLI) is a devastating disease in patients undergoing hemodialysis (HD). Based on the unsatisfactory results of autologous mononuclear cell transplantation for patients with CLI undergoing HD, we conducted a phase II clinical trial to evaluate the safety and efficacy of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood-derived autologous purified CD34 positive (CD34+) cell transplantation for CLI in patients undergoing HD. Six patients with CLI (two with Rutherford category 4 and four with Rutherford category 5) were enrolled...
July 30, 2018: Stem Cells Translational Medicine
Lu Wang, Kun Dou, Sungjin Moon, Frederick J Tan, Zz Zhao Zhang
Although animals have evolved multiple mechanisms to suppress transposons, "leaky" mobilizations that cause mutations and diseases still occur. This suggests that transposons employ specific tactics to accomplish robust propagation. By directly tracking mobilization, we show that, during a short and specific time window of oogenesis, retrotransposons achieve massive amplification via a cell-type-specific targeting strategy. Retrotransposons rarely mobilize in undifferentiated germline stem cells. However, as oogenesis proceeds, they utilize supporting nurse cells-which are highly polyploid and eventually undergo apoptosis-as factories to massively manufacture invading products...
July 20, 2018: Cell
Kiran Shah, Tara Drury, Ivelise Roic, Peter Hansen, Mark Malin, Richard Boyd, Huseyin Sumer, Ray Ferguson
Osteoarthritis is a common condition that causes joint pain and stiffness that affects both humans and dogs. In Australia, allogeneic canine adipose-derived mesenchymal stem cells for therapy have been commercially available since 2010. In this report, we describe the outcome of the treatment of two hundred and three dogs diagnosed with degenerative arthritis with severe chronic pain in joints causing lameness at walk, reduced mobility, and functional disability. Posttreatment assessment data after 10 weeks revealed significant improvement ( p < 0...
2018: Stem Cells International
H Michelle Grandin, Orane Guillaume-Gentil, Tomaso Zambelli, Michael Mayer, Jared Houghtaling, Cornelia G Palivan, Marcus Textor, Fredrik Höök
The genesis for this topical review stems from the interdisciplinary Biointerfaces International conference 2016 (BI 2016) in Zurich, Switzerland, wherein the need for advances in analytical tools was both expressed and addressed. Pushing the limits of detection for characterizing individual components, such as single proteins, single drug-delivery vehicles, or probing single living cells in a more natural environment, will contribute to the understanding of the complex biomolecular systems central to a number of applications including medical diagnostics, tissue engineering, and drug screening and delivery...
July 26, 2018: Biointerphases
Luís Leitão, Cecília J Alves, Inês S Alencastre, Daniela M Sousa, Estrela Neto, Francisco Conceição, Catarina Leitão, Paulo Aguiar, Graça Almeida-Porada, Meriem Lamghari
Selectively recruiting bone marrow (BM)-derived stem and progenitor cells to injury sites is a promising therapeutic approach. The coordinated action of soluble factors is thought to trigger the mobilization of stem cells from the BM and recruit them to lesions to contribute to tissue regeneration. Nevertheless, the temporal response profile of the major cellular players and soluble factors involved in priming the BM and recruiting BM-derived cells to promote regeneration is unknown. We show that injury alters the BM cellular composition, introducing population-specific fluctuations during tissue regeneration...
July 25, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Kosei Matsue, Kyoya Kumagai, Isamu Sugiura, Takayuki Ishikawa, Tadahiko Igarashi, Tsutomu Sato, Michihiro Uchiyama, Toshihiro Miyamoto, Takaaki Ono, Yasunori Ueda, Toru Kiguchi, Yoshinori Sunaga, Toru Sasaki, Kenshi Suzuki
The present study ( Identifier: NCT02221492) was conducted to assess the efficacy and safety of plerixafor for the mobilization and collection of haematopoietic stem cells (HSCs) for autologous transplantation in Japanese non-Hodgkin lymphoma (NHL) patients. In this randomized phase 2 study, patients received granulocyte-colony stimulating factor (G-CSF, filgrastim) 400 µg/m²/day for up to 8 days. Starting on the evening of day 4, patients received, for up to 4 days, either plerixafor (240 µg/kg/day) in the G-CSF+ plerixafor arm (GP arm) or G-CSF alone arm (G arm)...
July 24, 2018: International Journal of Hematology
Gretchen Lewis, Lauryn Christiansen, Jessica McKenzie, Min Luo, Eli Pasackow, Yegor Smurnyy, Sean Harrington, Philip Gregory, Gabor Veres, Olivier Negre, Melissa Bonner
Lentiviral vector (LVV)-mediated transduction of human CD34+ hematopoietic stem and progenitor cells (HSPCs) holds tremendous promise for the treatment of monogenic hematological diseases. This approach requires the generation of a sufficient proportion of gene-modified cells. We identified staurosporine, a serine/threonine kinase inhibitor, as a small molecule that could be added to the transduction process to increase the proportion of genetically modified HSPCs by overcoming a LVV entry barrier. Staurosporine increased vector copy number (VCN) approximately 2-fold when added to mobilized peripheral blood (mPB) CD34+ cells prior to transduction...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
Ardavan Abiri
Death by Plasmodium falsiparum, the leading cause of malaria, is going to remain a major obstacle among the infectious diseases. Plasmepsin aspartic proteases are key proteins in the pathogenesis of plasmodium species which break down the hemoglobin and exploit it as a source of amino acids. These enzymes are one of the favorite targeting agents for medicinal chemists to design new drugs. Plasmepsin proteins show a "flap" region in their N-terminal domain, predisposing them to a good "filler" drug with an exceptional affinity to this enzyme...
September 2018: Medical Hypotheses
Daryoush Shahbazi-Gahrouei, Batool Hashemi-Beni, Alireza Moradi, Maryam Aliakbari, Saghar Shahbazi-Gahrouei
Background: Today, using cellular phone and its harmful effects in human life is growing. The aim of this study is to investigate the effect of the global system for mobile communication (GSM) 900 MHz cellular phone radiofrequency waves on growth, morphology, and proliferation rate of mesenchymal stem cells and Michigan Cancer Foundation (MCF-7) cells within the specific distance and intensity. Methods: MCF-7 and human adipose-derived stem cells (HADSCs) were exposed to GSM cellular phones 900 MHz frequency with intensity of 354...
2018: International Journal of Preventive Medicine
Andrzej Eljaszewicz, Lukasz Bolkun, Kamil Grubczak, Malgorzata Rusak, Tomasz Wasiluk, Milena Dabrowska, Piotr Radziwon, Wojciech Marlicz, Karol Kamiński, Janusz Kloczko, Marcin Moniuszko
Background: Acute lymphoblastic leukemia (ALL) is a malignant disease of lymphoid progenitor cells. ALL chemotherapy is associated with numerous side effects including neutropenia that is routinely prevented by the administration of growth factors such as granulocyte colony-stimulating factor (G-CSF). To date, the effects of G-CSF treatment on the level of mobilization of different stem and progenitor cells in ALL patients subjected to clinically effective chemotherapy have not been fully elucidated...
2018: Stem Cells International
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