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Mobilized Stem Cell

Ling Xue, Yaru Chen, Xiaowen Tang, Juan Yao, Huimin Huang, Min Wang, Shixin Ye, Meng Wang, Min-Xin Guan
Mutations in mitochondrial DNA (mtDNA) have been associated with deafness and their pathophysiology remains poorly understood. In this study, we investigated the pathogenic mechanism of deafness-associated 7505A > G variant in the mitochondrial tRNASer(UCN) . The m.7505A > G variant affected the highly conserved adenine at position 11 (A11), disrupted the highly conserved A11-U24 base-pairing of DHU stem of tRNASer(UCN) and introduced a tertiary base pairing (G11-C56) with the C56 in the TΨC loop...
October 15, 2018: Mitochondrion
Germano Guerra, Fabio Perrotta, Gianluca Testa
Pulmonary vascular disorders (PVDs) include primary or secondary diseases who influence the right heart function. Several researches showed that stem and progenitor cells may represent a novel approach in treating pulmonary hypertension. In particular, circulating endothelial progenitor cells (EPCs) are mobilized either from the bone marrow and/or arteries to replace dysfunctional endothelial cells and restore blood perfusion to ischemic tissues. They may deliver paracrine signals to stimulate local angiogenesis or may be physically incorporated within neovessels...
October 17, 2018: Current Pharmaceutical Biotechnology
Kristine Doney, Kerry McMillen, Laura Buono, H Joachim Deeg, Ted Gooley
This retrospective analysis of 2503 adult (≥20 years of age) allogeneic hematopoietic cell transplantation (HCT) recipients assessed the effect of body mass index (BMI) on transplant outcome. Median patient age was 51.7 years. Both nonmalignant and malignant diagnoses were included. Patients received either a myeloablative (52%) or reduced-intensity (48%) conditioning regimen. Donors were either related (42%) or unrelated (58%). Cord blood recipients were excluded. G-CSF mobilized peripheral blood cells were the stem cell source in 86% of transplants...
October 10, 2018: Biology of Blood and Marrow Transplantation
Annette Künkele, Christopher Brown, Adam Beebe, Stephanie Mgebroff, Adam J Johnson, Agne Taraseviciute, Lisa S Rolczynski, Cindy A Chang, Olivia C Finney, Julie R Park, Michael C Jensen
Cytotoxic chemotherapy and radiation can render lymphocyte repertoires qualitatively and quantitatively defective. Thus, heavily treated patients are often poor candidates for the manufacture of autologous CAR-T cell products. In the USA and Europe, children with high-risk neuroblastoma undergo apheresis early in the course of their treatment to collect peripheral blood stem cells (PBSCs) for cryopreservation in preparation for high-dose chemotherapy followed by autologous stem cell rescue. Here, we investigate whether these cryopreserved chemotherapy and G-CSF-mobilized PBSCs can be utilized as starting material for CAR-T cell manufacturing...
October 10, 2018: Biology of Blood and Marrow Transplantation
Muhammad Shafiq, Lina Wang, Dengke Zhi, Qiuying Zhang, Kai Wang, Lianyong Wang, Dong-Hwee Kim, Deling Kong, Soo Hyun Kim
In situ blood vessel regeneration through host stem/progenitor cell mobilization may hold great promise for vascular reconstruction. Neuropeptide substance P (SP) has been shown to accelerate tissue repair by endogenous cell mobilization and recruitment. This study was aimed to evaluate the vascular regeneration potential of SP and heparin co-tethered vascular grafts. Polycaprolactone (PCL), PCL/SP-conjugated poly(L-lactide-co-ε-caprolactone) (PLCL-SP) (SP), and PCL/PLCL-SP/heparin-conjugated PLCL (Hep/SP) vascular grafts were implanted as rat abdominal aorta substitutes for up to 2 weeks and 4 weeks...
October 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Adam M Greenbaum, Damian J Green, Leona A Holmberg, Ted Gooley, Brian G Till, Lihua E Budde, Heather Rasmussen, Oliver W Press, Ajay K Gopal
Background: Bendamustine is a chemotherapeutic agent that has shown broad activity in patients with lymphoid malignancies. It contains both alkylating and nucleoside analog moieties, and thus, is not commonly used for stem cell mobilization due to concerns that it may adversely affect stem cell collection. Here we describe the lymphoma subset of a prospective, non-randomized phase II study of bendamustine, etoposide, and dexamethasone (BED) as a mobilization agent for lymphoid malignancies...
September 2018: Blood Research
Anis A Hamid, Sarah C Markt, Cécile Vicier, Kathleen McDermott, Paul Richardson, Vincent T Ho, Christopher J Sweeney
BACKGROUND: High-dose chemotherapy (HDCT) with autologous stem-cell transplantation (aSCT) has been a standard therapy for relapsed metastatic germ-cell tumors (GCTs) over the last 2 decades, but there have been many changes in practice over time with respect to stem-cell source, mobilization, and conditioning regimens. Mobilization is impaired with greater cisplatin exposure. PATIENTS AND METHODS: Patients with relapsed GCT who received HDCT/aSCT at Dana-Farber Cancer Institute between 1997 and 2017 were identified...
September 19, 2018: Clinical Genitourinary Cancer
Guanghui Xiu, Wei Xiong, Yunyu Yin, Xianzhong Chen, Ping Liu, Jie Sun, Bin Ling
OBJECTIVE: To determine the effect of bone mesenchymal stem cells (BMSCs) in transplantation therapy for lipopolysaccharide (LPS)-induced coagulation disorder and the underlying mechanism of high mobility group protein B1-receptors for advanced glycation end products/Toll-like receptors-nuclear factor-κB (HMGB1-RAGE/TLRs-NF-κB) signaling pathway. METHODS: BMSCs of female Sprague-Dawley (SD) rats ageing 4-5 weeks old were extracted and cultivated in vitro, and the fourth-passaged BMSCs phenotype was identified by flow cytometry for transplantation in the following experimental study...
September 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Jinwen Su, Ming Fang, Bei Tian, Jun Luo, Can Jin, Xuejun Wang, Zhongping Ning, Xinming Li
Apoptosis is involved in the death of cardiac progenitor cells (CPCs) after myocardial infarction (MI) in the heart. The loss of CPCs results in infarct scar and further deterioration of the heart function. Though stem cell-based therapy provides an effective approach for heart function recovery after MI, the retention of CPCs in the infarcted area of the heart is the main barrier that limits its promising therapy. Therefore, the underlying mechanisms of CPC apoptosis in hypoxia are important for the development of new therapeutic targets for MI patients...
October 11, 2018: Acta Biochimica et Biophysica Sinica
Angela Raucci, Stefania Di Maggio, Francesco Scavello, Alessandro D'Ambrosio, Marco E Bianchi, Maurizio C Capogrossi
High mobility group box 1 (HMGB1) is a ubiquitous nuclear protein involved in transcription regulation, DNA replication and repair and nucleosome assembly. HMGB1 is passively released by necrotic tissues or actively secreted by stressed cells. Extracellular HMGB1 acts as a damage-associated molecular pattern (DAMPs) molecule and gives rise to several redox forms that by binding to different receptors and interactors promote a variety of cellular responses, including tissue inflammation or regeneration. Inhibition of extracellular HMGB1 in experimental models of myocardial ischemia/reperfusion injury, myocarditis, cardiomyopathies induced by mechanical stress, diabetes, bacterial infection or chemotherapeutic drugs reduces inflammation and is protective...
October 10, 2018: Cellular and Molecular Life Sciences: CMLS
Kiran Shah, Ashley G Zhao, Huseyin Sumer
Osteoarthritis is one of the most common chronic health problems in the world that causes disability and chronic pain with reduced mobility and is a progressive degenerative disease in weight-bearing joints such as the knee. The pathology of the joint resulting from OA includes loss of cartilage volume and cartilage lesions leading to inflammation of the articular joint structures; its incidence and progression are associated with a variety of risk factors. Most of the current treatments focus on symptom management such as physical and occupational therapies, pharmacological intervention for pain management, and surgical intervention with limited success and do not address nor halt the progression of the disease...
2018: Stem Cells International
Nassim Hammoudi, Cédric Torre, Eric Ghigo, Michel Drancourt
Studies of tissue regeneration and host-pathogen interactions using the model planarian Schmidtea mediterranea have been performed at an experimental temperature of 19 °C. S. mediterranea planarians exposed to 19 °C-32 °C were observed for survival, mobility, feeding and regeneration for three months and elimination of the Staphylococcus aureus pathogen over six days. S. mediterranea planarians died at 30 °C-32 °C after 18 days of observation but tolerated temperatures of 19 °C up to 28 °C with non-significant differences in mobility and feeding behavior...
October 8, 2018: Scientific Reports
Lu Qu, Jingya Ruan, Song Wu, Peijian Huang, Jiejing Yan, Haiyang Yu, Yi Zhang, Tao Wang
In order to find a simple, generic, efficient separation method for 25 R / S -spirostanol saponin diastereomers, the liquid chromatographic retention behaviors of C12 carbonylation and C12 unsubstituted 25 R / S -spirostanol saponin diastereomers on different stationary phases (C₈, C18 , C30 columns) and different mobile phases (MeOH-1% CH₃COOH and CH₃CN-1% CH₃COOH) were investigated. A C30 column was firstly found to offer the highest efficiency for the separation of this kind of diastereomers than C₈ and C18 columns...
October 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Jingjing Liu, Yuxuan Xu, Qiaofang Wu, Qi Ding, Weixin Fan
OBJECTIVE: Stem cell transplantation is a promising tool to treat burn injuries. However, the inflammatory microenvironment in damaged skin limits the efficiency of stem cell-based therapy via poorly understood mechanisms. The aim of our study is to explore the contribution and mechanism of Sirtuin-1 (Sirt1) in TNFα-mediated inflammatory stress in hair follicle stem cells (HFSCs). METHODS: Cellular viability was determined using the MTT assay, TUNEL staining, western blot analysis and LDH release assay...
October 4, 2018: Life Sciences
Mona Vogel, Hannes Christow, Isabel Manz, Michael Denkinger, Amanda Amoah, Desiree Schütz, Andreas Brown, Bettina Möhrle, Annika Schaffer, Miriam Kalbitz, Florian Gebhard, Benjamin Mayer, Markus Huber-Lang, Hartmut Geiger
Endogenously mobilized stem and progenitor cells (SPCs) or exogenously provided SPCs are thought to be beneficial for trauma therapy. However, still little is known about the synchronized dynamics of the number of SPCs in blood after severe injury and parameters like cytokine profiles that correlate with these numbers. We determined the number of hematopoietic stem cells, common myeloid progenitors, granulocyte-macrophage progenitors and mesenchymal stem/stromal cells in peripheral blood (PB) 0-3 h, 8 h, 24 h, 48 h and 120 h after polytrauma in individual patients (injury severity score ≥ 21)...
October 4, 2018: Shock
Jiyin Zhou, Zuo Zhang, Guisheng Qian
Diabetes impairs the bone marrow (BM) architecture and function as well as the mobilization of immature cells into the bloodstream and number of potential regenerative cells. Circadian regulation of bone immature cell migration is regulated by β-adrenergic receptors, which are expressed on hematopoietic stem cells, mesenchymal stem cells, and osteoblasts in BM. Diabetes is associated with a substantially lower number of sympathetic nerve terminal endings in BM; thus, diabetic neuropathy plays a critical role in BM dysfunction...
October 5, 2018: Diabetes/metabolism Research and Reviews
Miriam Stenzinger, Halvard Bonig
Leukapheresis is like any other preparative apheresis, except it isn't: Leukapheresis typically takes much longer, larger blood volumes are processed and, consequently, larger ACD-A volumes are administered. Blood component donors and leukapheresis subjects are also quite different populations. Allogeneic donors tend to be younger and many are first-time donors, both of which are risk factors for adverse reactions during blood donation. Moreover, more than half of all leukapheresis collections are performed in patients...
September 15, 2018: Transfusion and Apheresis Science
Shin-Ichi Fuchida, Kazutaka Sunami, Morio Matsumoto, Hirokazu Okumura, Tohru Murayama, Toshihiro Miyamoto, Eichi Otsuka, Naohito Fujishima, Tohru Izumi, Shigehisa Tamaki, Yasushi Hiramatsu, Yoshiaki Kuroda, Chihiro Shimazaki, Koichi Akashi, Mine Harada
The efficacy and safety of lenalidomide (LEN) consolidation therapy and subsequent LEN maintenance therapy after high-dose therapy with autologous peripheral blood stem cell transplantation (auto-PBSCT) were evaluated in patients with newly diagnosed symptomatic multiple myeloma (MM). Forty-one patients were enrolled and received high-dose dexamethasone (DEX) therapy as an initial induction. The patients who did not respond to the DEX therapy were further treated with four cycles of bortezomib plus DEX (BD) induction therapy...
October 4, 2018: International Journal of Hematology
Erica B Esrick, John P Manis, Heather Daley, Cristina Baricordi, Hélène Trébéden-Negre, Francis J Pierciey, Myriam Armant, Sarah Nikiforow, Matthew M Heeney, Wendy B London, Luca Biasco, Mohammed Asmal, David A Williams, Alessandra Biffi
Novel therapies for sickle cell disease (SCD) based on genetically engineered autologous hematopoietic stem and progenitor cells (HSPCs) are critically dependent on a safe and effective strategy for cell procurement. We sought to assess the safety and efficacy of plerixafor when used in transfused patients with SCD for HSC mobilization. Six adult patients with SCD were recruited to receive a single dose of plerixafor, tested at lower than standard (180 µg/kg) and standard (240 µg/kg) doses, followed by CD34+ cell monitoring in peripheral blood and apheresis collection...
October 9, 2018: Blood Advances
Chaping Cheng, Zhongzhong Ji, Yaru Sheng, Jinming Wang, Yujiao Sun, Huifang Zhao, Xiaoxia Li, Xue Wang, Yuman He, Jufang Yao, Li Wang, Chenlu Zhang, Yanjing Guo, Jianming Zhang, Wei-Qiang Gao, Helen He Zhu
Tumor metastasis is the major cause of death for prostate cancer (PCa) patients. However, the treatment options for metastatic PCa are very limited. Epithelial-mesenchymal transition (EMT) has been reported to be an indispensable step for tumor metastasis and is suggested to associate with acquisition of cancer stem cell (CSC) attributes. We propose that small-molecule compounds that can reverse EMT or induce mesenchymal-epithelial transition (MET) of PCa cells may serve as drug candidates for anti-metastasis therapy...
2018: Theranostics
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