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https://www.readbyqxmd.com/read/30312877/periodontal-ligament-fibroblasts-migration-injury-via-ros-txnip-nlrp3-inflammasome-pathway-with-porphyromonas-gingivalis-lipopolysaccharide
#1
Dawei Lian, Linfeng Dai, Zhaoyu Xie, Xing Zhou, Xiaohong Liu, Yang Zhang, Yi Huang, Yang Chen
Inflammasomes serve as an intracellular machinery to initiate inflammatory response to various danger signals. However, the chronic periodontitis pathological relevance of this inflammasome activation, particularly in periodontal ligament fibroblasts, remains largely unknown. The present study demonstrated that Nlrp3 inflammasome components abundantly expressed in cultured mouse periodontal ligament fibroblasts (mPDLFs). In addition, our data demonstrated that P.g-LPS (Porphyromonas gingivalis Lipopolysaccharide), a major injurious factor during chronic periodontitis, could induce the mPDLFs migration dysfunction and the inhibition of Nlrp3 inflammasome by Isoliquiritigenin (ISO) markedly recovered the migration dysfunction in mPDLFs...
October 9, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/30312715/performance-of-the-flash-glucose-monitoring-system-during-exercise-in-youth-with-type-1-diabetes
#2
Elisa Giani, Maddalena Macedoni, Anna Barilli, Agnese Petitti, Chiara Mameli, Alessandra Bosetti, Andrea Cristiano, Dejan Radovanovic, Pierachille Santus, Gian Vincenzo Zuccotti
AIM: Metabolic changes during exercise may affect the accuracy of glucose sensors impacting on Type 1 diabetes (T1D) management. The present study aimed at assessing the performance of the Flash Glucose Monitoring system (isCGM) during exercise and in free-living condition in youth with T1D. METHODS: Seventeen youth (53% male), aged 13.7±3.8 years, with T1D for 5.4±3.8 years, HbA1c 7.4±1.0% (57±11 mmol/mol), were enrolled. Paired isCGM, plasma (PG) and capillary (CG) glucose values (total of 136) were collected during an interval exercise (45 min at 55% VO2max load with 20 seconds sprints at 80% VO2max every 10 minutes)...
October 9, 2018: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/30312180/validation-of-the-welch-allyn-pro-bp-2000-a-professional-grade-inflation-based-automated-sphygmomanometer-with-arrhythmia-detection-in-a-combined-pediatric-and-adult-population-by-ansi-aami-iso-standard-testing
#3
Bruce S Alpert
OBJECTIVE: The Welch Allyn Pro BP 2000, an automated sphygmomanometer, was subjected to ANSI/AAMI/ISO testing. PATIENTS AND METHODS: The protocol used was the same-arm sequential procedure described in the ANSI/AAMI/ISO 2013 Standard. Eight-eight (53 adults and 35 children aged 3-12 years old) patients completed testing successfully. Arm circumferences ranged from 15 to 49.5 cm. Seven different cuff sizes were used, including two long cuffs. RESULTS: All requirements for age, sex, blood pressure (BP), and cuff sizes were fulfilled...
October 11, 2018: Blood Pressure Monitoring
https://www.readbyqxmd.com/read/30311928/a-diglycolamide-functionalized-tren-based-dendrimer-with-a-crab-like-grip-for-the-complexation-of-actinides-and-lanthanides
#4
Arunasis Bhattacharyya, Andrea Leoncini, Prasanta K Mohapatra, Parveen K Verma, Avinash S Kanekar, Ashok K Yadav, Sambhunath Jha, Dibyendu Bhattacharyya, Richard J M Egberink, Jurriaan Huskens, Willem Verboom
A generation 1 dendrimer, based on tris(2-aminoethyl)amine (TREN), containing six diglycolamide (DGA) pendent arms (termed TREN-G1-DGA) was synthesized and evaluated for the extraction of actinides and fission product ions. Solvent extraction studies indicated preferential extraction of Eu3+ over Am3+ with a separation factor value of ca. 4.5 in line with the extraction behaviour of multiple DGA ligands in previous reports. The distribution values of Am3+ and Eu3+ were about 12 and 9 times higher, respectively, than those obtained in the case of TREN-DGA using the 1 × 10-3 M ligand in 5% iso-decanol/95% n-dodecane at 3 M HNO3...
October 12, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/30311639/implementation-of-planar-proton-minibeam-radiation-therapy-using-a-pencil-beam-scanning-system-a-proof-of-concept-study
#5
Ludovic De Marzi, Annalisa Patriarca, Catherine Nauraye, Eric Hierso, Rémi Dendale, Consuelo Guardiola, Yolanda Prezado
PURPOSE: Proton minibeam radiation therapy (pMBRT) is an innovative approach that combines the advantages of minibeam radiation therapy with the more precise ballistics of protons to further reduce the side effects of radiation. One of the main challenges of this approach is the generation of very narrow proton pencil beams with an adequate dose-rate to treat patients within a reasonable treatment time (several minutes) in existing clinical facilities. The aim of this study was to demonstrate the feasibility of implementing pMBRT by combining the pencil beam scanning (PBS) technique with the use of multislit collimators...
September 22, 2018: Medical Physics
https://www.readbyqxmd.com/read/30311149/cardiac-valve-bioreactor-for-physiological-conditioning-and-hydrodynamic-performance-assessment
#6
Brandon J Tefft, Joshua A Choe, Melissa D Young, Ryan S Hennessy, David W Morse, Jeffery A Bouchard, Herbert J Hedberg, Joseph F Consiglio, Dan Dragomir-Daescu, Robert D Simari, Amir Lerman
PURPOSE: Tissue engineered heart valves (TEHV) are being investigated to address the limitations of currently available valve prostheses. In order to advance a wide variety of TEHV approaches, the goal of this study was to develop a cardiac valve bioreactor system capable of conditioning living valves with a range of hydrodynamic conditions as well as capable of assessing hydrodynamic performance to ISO 5840 standards. METHODS: A bioreactor system was designed based on the Windkessel approach...
October 11, 2018: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/30311116/spatial-variability-and-seasonal-toxicity-of-dredged-sediments-from-guanabara-bay-rio-de-janeiro-brazil-acute-effects-on-earthworms
#7
Christiane Monte, Ricardo Cesar, Ana Paula Rodrigues, Danielle Siqueira, Aline Serrano, Leticia Abreu, Matheus Teixeira, Mariana Vezzone, Helena Polivanov, Zuleica Castilhos, Tácio de Campos, Glaucia G M Machado, Weber F Souza, Wilson Machado
The toxicity of dredged sediments from Guanabara Bay (Rio de Janeiro, Brazil) was evaluated using acute bioassays with Eisenia andrei and metal determination. The sediments were collected in August 2014 (winter) and February 2015 (summer) and in five areas distributed along the Bay: Port of Rio de Janeiro, Port of Niterói, Meriti River mouth, Iguaçu River mouth, and the Environmental Protection Area (APA) of Guapimirim. The sediments were mixed with a ferralsol (a representative Brazilian tropical soil) in proportions varying between 0 (pure soil) and 30%...
October 11, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/30308425/a-case-of-obstructive-jaundice-due-to-early-carcinoma-of-the-cystic-duct-protruding-into-the-common-bile-duct
#8
Yuhki Sakuraoka, Takashi Suzuki, Genki Tanaka, Takayuki Shimizu, Takayuki Shiraki, Park Kyongha, Shozo Mori, Yukihiro Iso, Masato Kato, Taku Aoki, Keiichi Kubota, Hidetsugu Yamagishi
INTRODUCTION: Cystic duct carcinoma is a rare disease, and only 33 cases reported worldwide have completely fulfilled the criteria first established by Farrar in 1951. Here we describe an extremely rare case of early cystic duct carcinoma that fulfilled the Farrar criteria, the papillary tumour protruding into the common bile duct, leading to obstructive jaundice. CASE PRESENTATION: A 76-year-old man visited a clinic with icteric conjunctivae, and was referred to our hospital for investigation of suspected obstructive jaundice...
October 4, 2018: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/30308238/%C3%AE-lipoic-acid-and-amlodipine-perindopril-combination-potentiate-the-therapeutic-effect-of-mesenchymal-stem-cells-on-isoproterenol-induced-cardiac-injury-in-rats
#9
Abeer I Abd El-Fattah, M S Zaghloul, N A Eltablawy, L A Rashed
Cardiac injury is a dangerous disease and become a greater issue in the forthcoming decades. The ultimate goal is to prevent the progression of heart failure and apoptotic processes. Cardiac tissue may regenerate itself but to certain extent depending on the number of resident stem cells that is limited. Thus, research had been focused on bone marrow derived stem cells (BM-MSCs) as a promising therapy in different types of tissues, including the heart. This study is designed not only to assess the therapeutic effect of BM-MSCs but also to improve their therapeutic effect in combination with antioxidant α-lipoic acid (ALA) and antihypertensive therapeutic drug form (AP) against isoproterenol-induced cardiac injury and compared with that of BM-MSCs alone...
October 8, 2018: Biochimie
https://www.readbyqxmd.com/read/30307844/are-bacterial-culture-quantifications-reliable-comparative-performance-of-the-wasp-automated-inoculation-instrument-in-the-era-of-iso-15189-accreditation
#10
V Fihman, S C Bleunven, J M Le Glaunec, F Maillebuau, I De Rochebouet, B Nebbad-Lechani, M Desroches, J W Decousser
PURPOSE: Isolating colonies and obtaining accurate colony counts from bacterial cultures are critical steps for the optimal management of infected patients. The uncertainties in the colony count results from the bacterial cultures were evaluated by verifying the performance of the WASP inoculation system according to the International Organization for Standardization (ISO) 15189 standard. METHODOLOGY: We first (i) evaluated the cross-contamination and precision of the WASP instrument (Copan Diagnostics, Italy) and (ii) established enumeration reading grids for urine, swab, bronchopulmonary specimens (BPSs) and catheter tip cultures...
October 11, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30307713/detection-of-homologue-and-isomer-vapors-through-dynamic-reflection-spectrum-of-hollow-mesoporous-silica-sphere-photonic-crystals
#11
Chengjia Xiong, Mengyao Pan, Lebin Wang, Hongbin Geng, Jiupeng Zhao, Yao Li
Homologues and isomers vapors with very similar refractive indices can be easily distinguished using dynamic reflection spectrum (DRS) of hollow mesoporous silica sphere (HMSS) photonic crystal (PC). Different diffusion behaviors render the HMSS PC with a distinct response to different chemicals vapors, leading to diversity in the evolution of reflection spectra and their DRS patterns. Therefore, by studying the geometric characteristics and color changes of color-filled contour map, as well as the reflection peak shift speeds at different stages, even isomers such as n-butanol and iso-butanol, with a refractive index difference of only 0...
October 11, 2018: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/30307389/pedobacter-chitinilyticus-sp-nov-a-chitin-degrading-bacterium-isolated-from-wheat-leaf-tissue
#12
Ling-Li Zhang, Long-Zhan Gan, Zhi-Bin Xu, Feng Yang, Yan Li, Xiao-Li Fan, Xiao-Feng Liu, Yong-Qiang Tian, Yu-Mei Dai
A bacterium, designated strain CM134L-2T , was isolated from a chitin-enriched wheat leaf microbiome in Chengdu, Sichuan province, China. It was Gram-stain-negative, strictly aerobic, non-spore-forming, motile, rod-shaped, and bright yellow in colour. Strain CM134L-2T grew at 4-35 °C, at pH 6.0-9.0 and could use chitin as the only carbon resource. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CM134L-2T was most closely related to Pedobacter nanyangensis Q-4T (97.7 %) and Pedobacter zeaxanthinifaciens TDMA-5T (97...
October 11, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/30307388/paenibacillus-montanisoli-sp-nov-isolated-from-mountain-area-soil
#13
Minghan Wu, Yuanyuan Zong, Wei Guo, Gejiao Wang, Mingshun Li
A Gram-stain-negative, aerobic, motile, endospore-forming and rod-shaped bacterium, designated RA17T , was isolated from Dafan, Hubei Province, China. Based on 16S rRNA gene sequence similarity comparisons, strain RA17T was most closely related to Paenibacillus taihuensis THMBG22T (97.4 %), Paenibacillus rhizoryzae ACCC 1ZS3-5T (97.4 %) and Paenibacillus sacheonensis DSM SY01T (96.5 %). Analysis of the rpoB gene also indicated that RA17T had the highest similarity to P. rhizoryzae ACCC 1ZS3-5T (92.3 %), P...
October 11, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/30306464/mucilaginibacter-formosus-sp-nov-a-bacterium-isolated-from-road-side-soil
#14
Md Amdadul Huq, Shahina Akter, Sun-Young Lee
A Gram-stain negative, aerobic, non-motile and rod-shaped novel bacterial strain, designated as MAH-5T , was isolated from a road-side soil sample and was characterised by using a polyphasic taxonomic approach. The colonies were observed to be yellowish orange in colour, smooth, circular and 0.3-0.7 mm in diameter when grown on nutrient agar for 2 days. Strain MAH-5T was found to be able to grow at 15-35 °C and at pH 4.0-8.0. The strain was observed to be positive for both the catalase and oxidase tests...
October 10, 2018: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/30305772/voxel-based-bed-and-eqd-2-evaluation-of-the-radiotherapy-treatment-plan
#15
Gaganpreet Singh, Arun S Oinam, Rose Kamal, Bhumika Handa, Vivek Kumar, Bhavana Rai
Introduction: Three-dimensional (3D) treatment planning of patient undergoing radiotherapy uses complex and meticulous computational algorithms. These algorithms use 3D voxel data of the patient to calculate the radiation dose distribution and display it over the CT image dataset for treatment plan evaluation. Aims and Objective: The purpose of the present study is the development and implementation of radiobiological evaluation of the radiotherapy treatment plan incorporating the tissue-specific radiobiological parameters...
July 2018: Journal of Medical Physics
https://www.readbyqxmd.com/read/30304816/risk-management-of-hazardous-materials-in-manufacturing-processes-links-and-transitional-spaces-between-occupational-accidents-and-major-accidents
#16
Francisco Brocal, Cristina González, Genserik Reniers, Valerio Cozzani, Miguel A Sebastián
Manufacturing processes involving chemical agents are evolving at great speed. In this context, managing chemical risk is especially important towards preventing both occupational accidents and major accidents. Directive 89/391/EEC and Directive 2012/18/EU, respectively, are enforced in the European Union (EU) to this end. These directives may be further complemented by the recent ISO 45001:2018 standard regarding occupational health and safety management systems. These three management systems are closely related...
October 9, 2018: Materials
https://www.readbyqxmd.com/read/30303477/streptomyces-lichenis-sp-nov-isolated-from-lichen
#17
Patcharin Saeng-In, Wongsakorn Phongsopitanun, Ancharida Savarajara, Somboon Tanasupawat
A new actinobacterial strain, LCR6-01T , was isolated from a lichen sample collected from Chiang Rai Province, Thailand. Its taxonomic position was determined using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus Streptomyces. The predominant menaquinones were MK-9(H6) and MK-9(H8). The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0, iso-C14 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified lipid...
October 10, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/30303476/hanstruepera-crassostreae-sp-nov-a-novel-marine-bacterium-of-the-family-flavobacteriaceae-isolated-from-an-oyster
#18
Rui-Han He, Qi-Yun Liang, Jin-Xin Zhao, Zong-Jun Du
A Gram-stain-negative, rod-shaped, non-motile, aerobic and orange-pigmented marine bacterium, designated strain L53T , was isolated from an oyster sample collected from the coast of Weihai, China (122.0° E 37.5° N). Growth of strain L53T occurred at 4-40 °C (optimum, 33 °C), at pH 6.5-8.0 (optimum, pH 7.5) and with 1.0-7.0 % (w/v) NaCl (optimum, 3.0 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain L53T was closely related to Bizionia echini KCTC 22015T (96.9 %) and Hanstruepera neustonica JCM 19743T (96...
October 10, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/30303472/streptomyces-durbertensis-sp-nov-isolated-from-saline-alkali-soil
#19
Yang Yu, Yansong Fu, Xiaowei Guo, Rui Yan, Han Wang, Junwei Zhao, Xiangjing Wang, Ji Zhang, Wensheng Xiang
A novel strain of actinobacteria, designated NEAU-S1GS20T , was isolated from a saline-alkali soil collected from Heilongjiang Province, north-east China, and characterized using a polyphasic approach. Strain NEAU-S1GS20T exhibited morphological, cultural and chemotaxonomic features consistent with its classification as representing a member of the genus Streptomyces. Growth occurred at 18‒45 °C, at pH 6.0‒10.0 and in the presence of 10 % (w/v) NaCl. Whole-cell hydrolysates mainly contained glucose and ribose...
October 10, 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/30303030/periostin-alters-transcriptional-profile-in-a-rat-model-of-isoproterenol-induced-cardiotoxicity
#20
M Sözmen, A K Devrim, Y B Kabak, T Devrim
Periostin is an extracellular matrix protein from the fasciclin family that guides cellular trafficking and extracellular matrix organization. Periostin stimulates mature cardiomyocytes to reenter the cell cycle. The molecular mechanism behind such stimulation remains to be explored. A DNA microarray technology constituting 30,429 gene-level probe sets was utilized to investigate effects of recombinant murine periostin peptide on the gene expression pattern in a rat model of isoproterenol (ISO)-induced myocardial injury...
October 10, 2018: Human & Experimental Toxicology
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