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Kiara Serafini Dapieve, Luí S Felipe Guilardi, Tais Silvestri, Marília Pivetta Rippe, Gabriel Kalil Rocha Pereira, Luiz Felipe Valandro
This study aims to evaluate the flexural fatigue strength and survival estimates of an Y-TZP monolithic ceramic after grinding and aging. Ceramic discs (1.2 mm thickness × 15.0 mm diameter - ISO, 6872, 2015) of zirconia (Zirlux FC2 - Ardent; Ivoclar Vivadent) were made and randomly allocated into 6 groups (n = 10), according to grinding and aging factors: Ctrl - as-sintered; Ctrl Sto - as-sintered and dry stored at room temperature for 2 years; Ctrl Aut Sto - as-sintered, submitted to autoclaved aging (134 °C, 2 bar, 20 h) and then dry stored for 2 years; and similar conditions for ground samples (Ground; Ground Sto; Ground Aut Sto)...
July 31, 2018: Journal of the Mechanical Behavior of Biomedical Materials
Kaori Shigeta, Ai Fujita, Tetsuya Nakazato, Hiroaki Tao
We have developed a method for the determination of Cr(VI) and Cr(III) in industrial wastewater by liquid chromatography-inductively coupled plasma mass spectrometry (LC-ICP-MS) combined with a chelating pretreatment with 2,6-pyridinedicarboxylic acid (PDCA). The PDCA unified the chemical forms of the Cr(III) species in water samples by the formation of a stable Cr(III)-PDCA complex, which was then separated by a LC column. The chromatographic mobile phase at neutral pH and the column of a mixed-bed of anion and cation exchangers successfully separated not only the chromium species without any redox conversion, but also chloride, which interfered with ICP-MS detection...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Eva Dohle, Smriti Singh, Akihiro Nishiguchi, Thorsten Fischer, Matthias Wessling, Martin Möller, Robert Sader, Jennifer Yvonne Kasper, Shahram Ghanaati, C James Kirkpatrick
The development of an in vitro model resembling the alveolar-capillary barrier might be a highly beneficial tool to study lung physiology as well as the immune response of the lung to infection or after exposure to nanoparticles. This study is based on an in vitro alveolar barrier developed on a basement membrane mimic, composed of ultrathin nanofiber meshes generated via electrospinning using bioresorbable poly(ε-caprolactone) (PCL). As cellular components, NCI H441, resembling the alveolar epithelial cells, and ISO-HAS-1, an endothelial cell line, were used to perform bipolar co-culture experiments for a total cultivation period of 14 days...
August 13, 2018: Tissue Engineering. Part C, Methods
P Cho, S Reyes, M V Boost
This study investigated the efficacy of a novel povidone-iodine based disinfection solution for rigid gas permeable (RGP) lenses compared to three other currently available multipurpose and hydrogen peroxide solutions. Bactericidal and fungicidal activities were assessed using ISO 14729 reference methods, whilst amoebicidal properties were evaluated using an automated viability counter. All solutions were also assessed for long term storage stability over a 3-month period. The FDA guidelines were adequately achieved by all solutions tested and 3-month storage did not affect their activity against bacterial and fungal agents...
August 9, 2018: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
Kelsey R Bilsback, S R Eilenberg, Nicholas Good, Lauren Heck, Michael Johnson, John K Kodros, Eric M Lipsky, Christian L'Orange, Jeffrey R Pierce, Allen L Robinson, R Subramanian, Jessica Tryner, Ander Wilson, John Volckens
Emissions from solid-fuel cookstoves have been linked to indoor and outdoor air pollution, climate forcing, and human disease. Although task-based laboratory protocols, such as the Water Boiling Test (WBT), overestimate the ability of improved stoves to lower emissions, WBT emissions data are commonly used to benchmark cookstove performance, estimate indoor and outdoor air pollution concentrations, estimate impacts of stove intervention projects, and select stoves for large-scale control trials. Multiple-firepower testing has been proposed as an alternative to the WBT and is the basis for a new standardized protocol (ISO 19867-1:2018); however, data are needed to assess the value of this approach...
August 11, 2018: Indoor Air
Michel Wathier, Benjamin A Lakin, Benjamin G Cooper, Prashant N Bansal, Alison M Bendele, Vahid Entezari, Hideki Suzuki, Brian D Snyder, Mark W Grinstaff
Intra-articular injection of hyaluronic acid (HA) is used to treat osteoarthritis (OA) as a viscosupplement, yet it only provides short-term benefit because HA is cleaved by hyaluronidase and cleared out of the joint after several days. Therefore, we developed a new polymer biolubricant based on poly-oxanorbornane carboxylate to enhance joint lubrication for a prolonged time. Rheological and biotribological studies of the biolubricant reveal viscoelastic properties and coefficient of friction equivalent and superior to that of healthy synovial fluid, respectively...
August 7, 2018: Biomaterials
Brian Young, Johann-Jakob Schmid
The current set of IEC particular standards that pertain to electrocardiograph (ECG) devices, namely, 60601-2-25, 60601-2-27, and 60601-2-47, which define requirements and testing for the essential performance and basic safety of diagnostic ECG, ECG monitoring and ambulatory ECG systems, respectively. These standards have been harmonized with the corresponding AAMI standards, namely EC11, EC13 and EC38. Together these standards have been in existence for decades and have evolved separately even though the technology used in these three clinical applications is very similar...
July 17, 2018: Journal of Electrocardiology
Zhizhi Wang, Wenqing Xu
The poly(ADP-ribose) polymerase (PARP) family of proteins utilize NAD+ as the substrate to modify protein acceptors with either mono(ADP-ribose) (MAR) or poly(ADP-ribose) (PAR). MAR and PAR have been shown to regulate distinct cellular processes. Iso-ADP-ribose (iso-ADPr) is the smallest internal PAR structural unit containing the characteristic ribose-ribose glycosidic bond formed during poly(ADP-ribosyl)ation. The WWE domain of RNF146 specifically recognizes the iso-ADPr moiety in PAR but does not interact with MAR...
2018: Methods in Molecular Biology
Xiang Yan, Yong-Xiang Xu, Yang Wu, Hong Lin
STATEMENT OF PROBLEM: Components fabricated by selective laser melting (SLM) deform because of residual stress, but heat treatment allows the release of that stress and avoids deformation. Although dental cobalt-chromium (Co-Cr) alloy has been specifically designed for SLM, the effects of heat treatment on the metal-ceramic combination of SLM Co-Cr restorations require investigation. PURPOSE: The purpose of this in vitro study was to evaluate the effects of heat treatment on the metal-ceramic combination of SLM Co-Cr alloy...
August 2018: Journal of Prosthetic Dentistry
Juncheng Wang, Baochun Li, Lirong Yao, Yaxiong Meng, Xiaole Ma, Yong Lai, Erjing Si, Panrong Ren, Ke Yang, Xunwu Shang, Huajun Wang
Compartmentalization of Na+ into vacuoles is considered to be the most critical aspect of salt tolerance in H. glomeratus, an annual, succulent halophyte. Previous analysis of transcriptome involved in the H. glomeratus salt stress response relied on next-generation sequencing technologies that limit the capture of accurately spliced, full-length isoforms. To gain deeper insights into its salt stress response, we used the H. glomeratus Iso-Seq transcriptome database as a reference, and subsequent next-generation sequencing was subjected to various NaCl concentrations of leaves from plants revealed 115 upregulated and 87 downregulated differentially expressed isoforms (core DEIs)...
August 7, 2018: Gene
Stanislava Králová, Pavel Švec, Hans-Jürgen Busse, Eva Staňková, Peter Váczi, Ivo Sedláček
A group of rod-shaped, aerobic, Gram-stain-negative, gliding bacteria producing flexirubin-type pigment was isolated from environmental samples collected in Antarctica in 2009-2014. Phylogenetic analysis of the almost complete 16S rRNA gene sequences revealed two separated branches belonging to the genus Flavobacterium. Group I (n=8), represented by strain CCM 8826T , shared the highest sequence similarity to Flavobacterium collinsii 983-08T (98.8 %) and Flavobacterium saccharophilum DSM 1811T (98.4 %), and group II (n=4) represented by strain CCM 8827T shared the highest similarity to Flavobacterium aquidurense WB 1...
August 10, 2018: International Journal of Systematic and Evolutionary Microbiology
Susana Cuello-Nuñez, Jocelyn Benning, Chuan Liu, Peter Branton, Jin Hu, Kevin G McAdam, Steven Coburn, Julian Braybrook, Heidi Goenaga-Infante
Fractionation data for cadmium in tobacco products, as obtained by sequential leaching of cadmium species with ICP-MS/MS analysis, and separately by X-ray absorption near edge structure (XANES) are presented here for the first time. The total amount of cadmium found in 3R4F cigarette cut tobacco was 1526 ± 42 μg kg-1 , of which 5% was found in the smoke under ISO smoking conditions. XANES analysis showed that Cd in tobacco, cigarette smoke and ash was present in the + 2 oxidation state. Examination of the gas-particle partitioning of smoke cadmium suggests that Cd in mainstream smoke is best viewed as semi-volatile, existing in both particulate and gas phases...
August 10, 2018: Analytical and Bioanalytical Chemistry
Cécile Morlot, Daniel Straume, Katharina Peters, Olav A Hegnar, Nolwenn Simon, Anne-Marie Villard, Carlos Contreras-Martel, Francisco Leisico, Eefjan Breukink, Christine Gravier-Pelletier, Laurent Le Corre, Waldemar Vollmer, Nicolas Pietrancosta, Leiv Sigve Håvarstein, André Zapun
The universality of peptidoglycan in bacteria underlies the broad spectrum of many successful antibiotics. However, in our times of widespread resistance, the diversity of peptidoglycan modifications offers a variety of new antibacterials targets. In some Gram-positive species such as Streptococcus pneumoniae, Staphylococcus aureus, or Mycobacterium tuberculosis, the second residue of the peptidoglycan precursor, D-glutamate, is amidated into iso-D-glutamine by the essential amidotransferase MurT/GatD complex...
August 9, 2018: Nature Communications
Jianbin Yan, Ruifeng Yao, Li Chen, Suhua Li, Min Gu, Fajun Nan, Daoxin Xie
Jasmonates (JAs) are cyclic fatty acid-derived phytohormones that regulate diverse aspects of plant defense and development. The endogenous active JA molecule (+)-7-iso-JA-L -Ile (JA-Ile) and its analog coronatine trigger formation of the complex with the F-box protein COI1 and JAZ repressors to induce degradation of JAZs through 26S proteasome pathway in a COI1-dependent manner. To reveal the formation process of COI1-JA-JAZ ternary complex, we employed comprehensive biochemical approaches to examine how JA is dynamically perceived...
August 6, 2018: Molecular Plant
Rudolf Spunda, Jan Hruby, Pavel Mericka, Mikulas Mlcek, Ondrej Pecha, Kathrin Splith, Moritz Schmelzle, Felix Krenzien, Jaroslav Lindner, Ivan Matia, Miroslav Spacek
OBJECTIVES AND DESIGN: The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. MATERIALS AND METHODS: Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively...
2018: PloS One
L Martin, M Audran, A Marchand
Iso-Electric Focusing (IEF) was the first method established to discriminate endogenous and recombinant erythropoietins (rEPOs) and is still approved by the World Anti-Doping Agency (WADA) as Initial Testing Procedure to detect erythropoiesis stimulating agents (ESAs) in doping control samples. However EPO-Fc, one of the prohibited rEPOs designated by WADA, is not detectable with the actual IEF conditions. Other newly developed ESAs luspatercept and sotatercept, both activin receptor type II-Fc fusion proteins (ActRII-Fc), are also now prohibited and could be used in combination with rEPOs...
August 9, 2018: Drug Testing and Analysis
Wen-Nan Yu, Ji-Ru Han, Yan Liu, Zong-Jun Du, Da-Shuai Mu
A Gram-stain-negative, non-motile, aerobic, curved-rod bacterium, designated as strain 2p52T , was isolated from the marine algae Gracilaria blodgettii collected off the coast of Lingshui county, in Hainan province, China. Strain 2p52T grew at 15-42 °C (optimally at 30-33 °C), at pH 6.0-10.0 (7.5-8.0) and in the presence of 1.0-8.0 % (w/v) NaCl (2.0-3.0 %). The most closely related species was Agaribacter marinus (96.5 % 16S rRNA gene sequence similarity). Phylogenetic analysis based on the sequence of the 16S rRNA gene revealed that strain 2p52T belonged to the genus Agaribacter...
August 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Chengliang Cao, Tangyu Xu, Jinjuan Liu, Xiaorui Cai, Yong Sun, Sheng Qin, Jihong Jiang, Ying Huang
A Gram-positive, strictly aerobic actinobacterium, designated BMP B8004T , was isolated from desert soil collected in Xinjiang Province, Northwest China. It produced an extensively branched non-fragmenting substrate mycelium, and very scanty aerial mycelium that formed a short hooked chain of arthrospores with a smooth surface. Optimum growth occurred at 28 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BMP B8004T formed a distinct phyletic lineage within the genus Actinomadura...
August 9, 2018: International Journal of Systematic and Evolutionary Microbiology
Gijs Ijpma, Liang Chu Qiao, Linda Kachmar, Alice Panariti, Andrea Benedetti, Jean-Pierre Lavoie, Anne-Marie Lauzon
RATIONALE: Isolated human airway smooth muscle (ASM) tissue contractility studies are essential for understanding the role of ASM in respiratory disease, but limited availability and cost render storage options necessary for optimal use. However, to date no comprehensive study of cryopreservation protocols for isolated ASM has been performed. METHODS: We tested several cryostorage protocols on equine trachealis ASM, using different cryostorage media (1.8M dimethyl sulfoxide and fetal bovine serum [FBS] or Krebs-Henseleit [KH]) and different degrees of dissection (with or without epithelium and connective tissues attached) prior to storage...
August 9, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
M Infas H Mohideen, Youssef Belmabkhout, Prashant M Bhatt, Aleksander Shkurenko, Zhijie Chen, Karim Adil, Mohamed Eddaoudi
Separation of paraffin isomers is of great importance in the refining industry because of their potential applications for energy efficiency, as reflected by their associated Research Octane Number (RON) values. Here, we report the synthesis of the first zeolite-like metal-organic framework (ZMOF) with ana topology that displays helicoidally/cylindrically-shaped channels with a pore-aperture size of ca. 3.8 × 6.2 Å. Markedly, such structural features offer potential for the selective separation of linear, and mono- and dibranched paraffins...
August 9, 2018: Chemical Communications: Chem Comm
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