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Greg Finak, Wenxin Jiang, Raphael Gottardo
CytoML is an R/Bioconductor package that enables cross-platform import, export, and sharing of gated cytometry data. It currently supports Cytobank, FlowJo, Diva, and R, allowing users to import gated cytometry data from commercial platforms into R. Once data are available in R, the data can be further manipulated. For example it can be combined with other computational and analytic approaches, and the results can be exported to FlowJo or Cytobank to be explored by researchers using those platforms. We demonstrate how CytoML and related R packages can be used as a tool to import, modify and export several samples stained with the T cell panel from the FlowCAP IV Lyoplate data set...
December 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Martin Freesmeyer, Wolfgang Weigand, Thomas Weisheit
AIM: In this contribution we investigated the potential transfer of an established method for the synthesis of 223 Ra-labelled calixarene complexes to a hospital radiopharmaceutical environment. For this purpose, commercially available [223 Ra]RaCl2 solution in pharmaceutical grade (Xofigo® ) was reacted with three calixarene derivatives. METHODS: A wellestablished two-phase extraction method using a two-phase solvent system was performed for complexation of the radium ions with calixarenes under slightly basic conditions...
December 2018: Nuklearmedizin. Nuclear Medicine
Houman Tahmasebi, Victoria Higgins, Amy Woroch, Shervin Asgari, Khosrow Adeli
INTRODUCTION: Accurate reference intervals (RIs) are essential for clinical interpretation of laboratory test results; however, major gaps exist in pediatric RIs. The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has established age- and sex-specific pediatric RIs on various analytical platforms. The current study expands the CALIPER database by establishing age- and sex-specific RIs for biochemical assays on Siemens ADVIA XPT/1800 and Dimension EXL Systems...
December 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Silvia Galbiati, Francesco Damin, Valentina Burgio, Angela Brisci, Nadia Soriani, Bernadette Belcastro, Chiara Di Resta, Luca Gianni, Marcella Chiari, Monica Ronzoni, Maurizio Ferrari
A major effort has been focused on the detection of oncogenes' mutations in diverse types of clinical specimens including formalin-fixed and paraffin embedded tissues, presently the gold-standard samples, up to plasma, that constitute a noninvasive alternative source of tumor DNA. The reliable detection of mutations in circulating tumor DNA requires a high analytical sensitivity. Here, we applied three different highly sensitive methodologies (COLD-PCR, a microarray-based approach and the droplet digital PCR, ddPCR) to identify mutations in the plasma of 30 metastatic colorectal cancer patients previously genotyped on tissue biopsy...
December 11, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Qiu-Da Zheng, Zhe Wang, Chun-Ye Liu, Ji-Hao Yan, Wei Pei, Zhuang Wang, De-Gao Wang
Wastewater-based epidemiology combining with a population model based on hydrochemical parameters was applied in 10 wastewater treatment plants (WWTPs) in Jilin province, China. Population of WWTPs served was calculated by the model including three hydrochemical parameters ammonia nitrogen, chemical oxygen demand, and total phosphorus. The population model was constructed by using analytic hierarchy process to calculate weight factors of each hydrochemical parameters equivalent population. The size of population estimated by the model showed the highest correlations with cotinine mass load (r2  = 0...
November 29, 2018: Science of the Total Environment
Yan Wang, Shiming Ding, Mingyi Ren, Cai Li, Shiwei Xu, Qin Sun, Ligang Xu
There is a requirement for simultaneous measurements of diverse analytes to reveal their biogeochemical couplings in the environment. In this study, we first realized simultaneous measurements of three types of analytes, including sulfide [S(-II)], metallic cations, and oxyanions by diffusive gradients in thin films (DGT) using a new mixed ZrO-CA binding gel. The ZrO-CA gel exhibited faster absorption rates for oxyanions than previously reported ZrO-Chelex gel, and faster absorption rates for cations when the gel was saturated with S(-II)...
December 4, 2018: Science of the Total Environment
Kai-Ge Lu, Haiou Huang
Silica scaling of reverse osmosis membranes in brackish water desalination is less understood than hardness scaling due to the complex silica behaviors at the membrane/water interface. In this study, -COOH, -SO3 H, -NH2 and -OH functional groups were introduced onto polyamide membranes to create distinct surface physicochemical properties. The resulting membranes were further studied under similar scaling conditions to yield temporal flux loss data that were empirically interpreted by a logistic growth model...
November 30, 2018: Water Research
Peter G Lawlor, Nicole A Rutkowski, Alistair R MacDonald, Mohammed T Ansari, Lindsey Sikora, Franco Momoli, Salmaan Kanji, David K Wright, Erin Rosenberg, Annmarie Hosie, Jose L Pereira, David Meagher, Jill Rice, John Scott, Shirley H Bush
CONTEXT: Based on the clinical care pathway of delirium in palliative care (PC), a published analytic framework (AF) formulated research questions in key domains and recommended a scoping review to identify evidence gaps. OBJECTIVES: To produce a literature map for key domains of the published AF: screening; prognosis and diagnosis; management and the health-related outcomes. METHODS: A standard scoping review framework was used by an interdisciplinary study team of nurse and physician delirium researchers, an information specialist and review methodologists to conduct the review...
December 11, 2018: Journal of Pain and Symptom Management
Thomas S O'Leary, Julia Snyder, Sakthivel Sadayappan, Sharlene M Day, Michael J Previs
RATIONALE: Truncation mutations in the MYBPC3 gene, encoding for cardiac myosin-binding protein C (MyBP-C), are the leading cause of hypertrophic cardiomyopathy (HCM). Whole heart, fiber and molecular studies demonstrate that MyBP-C is a potent modulator of cardiac contractility, but how these mutations contribute to HCM is unresolved. OBJECTIVES: To readdress whether MYBPC3 truncation mutations result in loss of MyBP-C content and/or the expression of truncated MyBP-C from the mutant allele and determine how these mutations effect myofilament sliding in human myocardium...
December 11, 2018: Journal of Molecular and Cellular Cardiology
Paul A Klaczynski, Wejdan Felmban
To explore hypotheses based on Stanovich's proposal that analytic processing comprises a reflective-level, an algorithmic level, and specific mindware, 342 participants completed measures of thinking dispositions, general ability (GA), numeracy, and probabilistic and nonprobabilistic reasoning. In a control condition, numeracy predicted probabilistic reasoning at high levels of both thinking dispositions and GA, and GA predicted nonprobabilistic reasoning at high levels of thinking dispositions. In a logic instruction condition, numeracy predicted probabilistic reasoning when GA was high, and GA affected nonprobabilistic reasoning directly...
December 14, 2018: Psychological Reports
(no author information available yet)
Reports an error in "Wisdom, bias, and balance: Toward a process-sensitive measurement of wisdom-related cognition" by Justin P. Brienza, Franki Y. H. Kung, Henri C. Santos, D. Ramona Bobocel and Igor Grossmann ( Journal of Personality and Social Psychology , Advanced Online Publication, Sep 21, 2017, np). In the article, the original supplemental material has been revised to include a clarifying note to the Tests of model fit over larger sample (Samples C-G) section and post-peer review analyses added to the Post-peer review Factor Analytic Tests section...
December 2018: Journal of Personality and Social Psychology
Amber E Witherby, Sarah K Tauber, Matthew G Rhodes, Alan D Castel
Recently, researchers have evaluated the mechanisms that contribute to younger adults' metacognitive monitoring. According to analytic-processing theory, people's beliefs about their memory are central to their monitoring judgments. Although this theory has received ample support with younger adults, it has yet to be evaluated with older adults. We aimed to address this gap in the literature. Specifically, we evaluated younger and older adults' beliefs about forgetting, and the role of these beliefs in their judgments about forgotten information...
December 13, 2018: Psychology and Aging
Benedek Kurdi, Allison E Seitchik, Jordan R Axt, Timothy J Carroll, Arpi Karapetyan, Neela Kaushik, Diana Tomezsko, Anthony G Greenwald, Mahzarin R Banaji
Using data from 217 research reports (N = 36,071, compared to 3,471 and 5,433 in previous meta-analyses), this meta-analysis investigated the conceptual and methodological conditions under which Implicit Association Tests (IATs) measuring attitudes, stereotypes, and identity correlate with criterion measures of intergroup behavior. We found significant implicit-criterion correlations (ICCs) and explicit-criterion correlations (ECCs), with unique contributions of implicit (β = .14) and explicit measures (β = ...
December 13, 2018: American Psychologist
Alexey S Galushko, Evgeniy G Gordeev, Valentine P Ananikov
A thermally induced cascade process leading to the formation of stable micro- and nanometer-size phosphoric droplets was developed starting from a molecular precursor. Microwave-induced pyrolysis of 1,2,3,4,5-pentaphenylphosphole oxide proceeded through a series of subsequent transformations involving formation of phosphorus-doped graphene oxide layers, seeding of carbon surface with phosphorus centers, and assembling of stable droplets. A complex nanostructured organization of the material was established in a remarkably short time of 3 min, and the process was performed in a thermally induced manner using microwave irradiation...
December 14, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Tanping Li, Xiaofang Wang
Correlations between the nonequilibrium solvation dynamics upon the photon excitation of the chromophore and system's equilibrium fluctuations are deeply studied. As linear response of the solvent has been linked with Gaussian statistics of the energy fluctuations in literatures, we specifically explore the cases beyond the regime of the linear response theory due to deviation from Gaussian fluctuations. As a continuation of our previous work, an analytical formalism is presented to project the energy shift with various order moments, where the non-Gaussian statistics arise from the overlap of the energy basins on the perturbed potential energy surface...
December 14, 2018: Journal of Chemical Theory and Computation
Hao Wang, Weitao Yang
Accurate force fields are crucial for molecular dynamics investigation of complex biological systems. Building accurate protein force fields from quantum mechanical (QM) calculations is challenging due to the complexity of proteins and high computational costs of QM methods. In order to overcome these two difficulties, here we developed the residue-based systematic molecular fragmentation method to partition general proteins into only twenty types of amino acid dipeptides and one type of peptide bond at Level 1...
December 14, 2018: Journal of Chemical Theory and Computation
Fabio Manoni, Gianluca Gessoni, Giovanni Battista Fogazzi, Maria Grazia Alessio, Alberta Caleffi, Giovanni Gambaro, Sandra Secchiero, Barbara Pieretti, Cosimo Ottomano, Anna Liverani, Cettina Drago, Fiamma Balboni, Maria Grazia Epifani, Graziella Saccani, Giovanni DI Rienzo, Sara Valverde, Rudi Ravasio, Giuliano Brunori, Loreto Gesualdo
With these recommendations the Interdisciplinary Urinalysis Group (GIAU) aims to stimulate the following aspects : improvement and standardization of the post analytical approach to physical, chemical and morphological urine examination (ECMU); emphasize the value added to ECMU by selection of clinically significant parameters, indication of analytical methods, of units of measurement, of reference values; improvement of interpretation of dip stick urinalysis with particular regard to the reconsideration of the diagnostic significance of the evaluated parameters together with an increasing awareness of the limits of sensitivity and specificity of this analytical method...
December 2018: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
Magdalena Fortova, Eva Klapkova, Bruno Sopko, Richard Prusa
<i>Background:</i> Many studies have reported higher values of urinary albumin measured by high performance liquid chromatography (HPLC) in comparison with immunochemical methods. The aims of our study were the implementation of the HPLC method for albuminuria, testing the hypothesis about coeluting proteins, comparison of albuminuria assessed by HPLC and immunoturbidimetric (IT) methods in diabetic and non-diabetic patient samples. <i>Methods:</i> We compared albuminuria assessed by HPLC with albuminuria assessed by the IT method in fresh urine samples of 636 diabetics and 456 non-diabetics...
October 31, 2018: Clinical Laboratory
Isabelle E Bauer, Charles Green, Gabriela D Colpo, Antonio L Teixeira, Sudhakar Selvaraj, Katherine Durkin, Giovana B Zunta-Soares, Jair C Soares
OBJECTIVE: Neuroinflammation has been implicated in the pathophysiology of bipolar disorder. Some evidence shows that nonsteroidal anti-inflammatory drugs (NSAIDs) have promising antidepressant effects. The antioxidant N-acetylcysteine (NAC) may enhance the effects of NSAIDs. No study has, however, tested the adjunctive therapeutic benefits of an NSAID and NAC in bipolar disorder. METHODS: The sample included 24 medicated patients diagnosed with DSM-IV-TR bipolar disorder who were aged 18-65 years and had a Montgomery-Asberg Depression Rating Scale (MADRS) score ≥ 20...
December 4, 2018: Journal of Clinical Psychiatry
G De-Deus, D M Cavalcante, F G Belladonna, J Carvalhal, E M Souza, R T Lopes, M A Versiani, E J N L Silva, P M H Dummer
AIM: To investigate the prevalence, location, and pattern of preexisting microcracks in non-endodontically treated teeth from fresh cadavers. Micro-computed tomography (micro-CT) technology was used as the analytical tool enabling full screening of the root dentine with the teeth retained in their original alveolar socket. METHODOLOGY: As a pilot study and to validate the present method, a series of 4 high-resolution scans were performed on one bone-block specimen with teeth collected postmortem: (i) entire bone-block including the teeth, (ii) second molar tooth extracted atraumatically from the bone-block (iii) extracted tooth dehydrated to induce dentinal defects and (iv) entire bone-block following reinsertion of the extracted tooth into its matching alveolar socket...
December 14, 2018: International Endodontic Journal
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