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https://www.readbyqxmd.com/read/30317506/use-of-surface-plasmon-resonance-spr-to-determine-binding-affinities-and-kinetic-parameters-between-components-important-in-fusion-machinery
#1
Robert P Sparks, Jermaine L Jenkins, Rutilio Fratti
Surface plasmon resonance (SPR) can be used to analyze both binding affinities and kinetic parameters between a ligand and an analyte. SPR can be performed by either cross-linking a given ligand to a sensor chip covalently or utilizing high-affinity non-covalent interactions to secure a ligand in a particular conformation to a chip, both of which have their potential advantages. SPR measurements are mass based and reflect the proportional amount of analyte bound to a given ligand at a given concentration when flowed at a set rate in order to determine the binding parameters of a given biochemical interaction...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30317505/use-of-microscale-thermophoresis-mst-to-measure-binding-affinities-of-components-of-the-fusion-machinery
#2
Robert P Sparks, Rutilio Fratti
Microscale thermophoresis is a relatively new technique used by an increasing number of academic laboratories to estimate relative binding affinities between ligand (analyte) that is titrated and a target (generally protein) that is either fluorescently labeled exogenously in the red or blue channel (labeled thermophoresis) or endogenously labeled via the presence of sufficient aromatic amino acid residues such as tryptophan (label-free thermophoresis). There are advantages and disadvantages to each technique; however, one major disadvantage of label-free thermophoresis is that protein-protein interactions cannot be measured, as generally most proteins have enough aromatic residues to generate an interfering signal...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30317445/new-structural-insights-into-the-role-of-trove2-complexes-in-the-on-set-and-pathogenesis-of-systemic-lupus-erythematosus-determined-by-a-combination-of-qcm-d-and-dpi
#3
Augusto Juste-Dolz, Noelle M do Nascimento, Isidro Monzó, Elena Grau-García, Jose A Román-Ivorra, José Luis Lopez-Paz, Jorge Escorihuela, Rosa Puchades, Sergi Morais, David Gimenez-Romero, Ángel Maquieira
The mechanism of self-recognition of the autoantigen TROVE2, a common biomarker in autoimmune diseases, has been studied with a quartz crystal microbalance with dissipation monitoring (QCM-D) and dual polarization interferometry (DPI). The complementarity and remarkable analytical features of both techniques has allowed new insights into the onset of systemic lupus erythematosus (SLE) to be achieved at the molecular level. The in vitro study for SLE patients and healthy subjects suggests that anti-TROVE2 autoantibodies may undergo an antibody bipolar bridging...
October 13, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30317444/profiling-of-nanoparticle-protein-interactions-by-electrophoresis-techniques
#4
REVIEW
Mohammad Zarei, Jamal Aalaie
The use of nanomaterials in the chemical, biomedical, and biotechnological sciences is growing, increasing the possible release of these materials into the environment and contact with living organisms. Because of their large surface area, biomolecules can be adsorbed on the surface of nanomaterials. Proteins bind to the surface of nanoparticles (NPs), forming a biological layer around the NP, the "protein corona," which gives new characteristics to the NPs in biological milieu and may affect biomedical applications or induce nanotoxicological effects...
October 13, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30317382/simultaneous-determination-of-32-polycyclic-aromatic-hydrocarbon-derivatives-and-parent-pahs-using-gas-chromatography-mass-spectrometry-application-in-groundwater-screening
#5
Tao Zhu, Zhu Rao, Feng Guo, Nan Zhan, Yuan Wang, Hamidreza Arandiyan, Xiao-Jie Li
A simple, practical and precise method for the simultaneous analysis of 32 different polycyclic aromatic hydrocarbon (PAHs) including 16 parent PAHs, 8 oxygenated-PAHs (oxy-PAHs), 4 chloro-PAHs, and 4 nitrogen-containing heterocyclic PACs (N-PACs), in groundwater was established via gas chromatography-mass spectrometry (GC-MS) combined with liquid-liquid extraction (LLE). The obtained detection method possesses instrument detection limits (at a signal to noise of 3:1) in the range of 0.05-10 ng/mL and method detection limits in the range of 1...
October 13, 2018: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/30317270/forecasting-incidence-of-dengue-and-selecting-best-method-for-prevention
#6
Muhammad Sabir, Yousaf Ali, Noor Muhammad
This study forecast future infected patients from dengue and also sought optimum solution for its prevention. Previous years' data of dengue patients was used for forecasting numbers of infected patients in 2017. The novelty of the study is the application of Analytical Hierarchy Process (AHP) technique in application of dengue control. We employed AHP to rank the alternative for dengue prevention using perception of general public collected via questioners from 100 respondents across Pakistan. The results show that public considered not to let the water accumulate inside their homes as the most economical, effective, efficient and less labour intensive alternative to combat dengue...
September 2018: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/30317268/evaluation-of-correlation-between-expression-of-p53-and-malondialdehyde-levels-in-prostate-cancer-patients
#7
Maria Arif, Amir Rashid, Asifa Majeed, Fatima Qaiser, Suhail Razak
The analytical study was conducted at the National University of Sciences and Technology, Islamabad, Pakistan from Nov 2012 to Nov 2013 to find out, correlate and assess negative correlation of serum malondialdehyde (MDA) with expression of p53 gene, and comprised 32 samples. Expression of p53 and MDA levels were determined by real time quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA) technique respectively. Mean value of MDA in prostate carcinoma (CaP) and control group were compared, and the difference was statistically significant (p=0...
September 2018: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/30317120/comparative-analysis-of-multiple-techniques-for-developing-and-transferring-safety-performance-functions
#8
Ahmed Farid, Mohamed Abdel-Aty, Jaeyoung Lee
Safety performance functions (SPFs) are crash count prediction models that are used for identifying high crash risk locations, evaluating road safety before and after countermeasure deployment and comparing the safety of alternative site designs. The traditional method of modeling crash counts is negative binomial (NB) regression. Furthermore, the Highway Safety Manual (HSM) provides analytical tools, including NB SPFs, to assess and improve road safety. Even though the HSM's SPFs are restricted to NB models, the road safety literature is rich with a variety of different modeling techniques...
October 11, 2018: Accident; Analysis and Prevention
https://www.readbyqxmd.com/read/30317099/association-between-deepwater-horizon-oil-spill-response-and-cleanup-work-experiences-and-lung-function
#9
Kaitlyn B Gam, Lawrence S Engel, Richard K Kwok, Matthew D Curry, Patricia A Stewart, Mark R Stenzel, John A McGrath, W Braxton Jackson, Maureen Y Lichtveld, Dale P Sandler
INTRODUCTION: Oil spill response and cleanup (OSRC) workers had potentially stressful experiences during mitigation efforts following the 2010 Deepwater Horizon disaster. Smelling chemicals; skin or clothing contact with oil; heat stress; handling oily plants/wildlife or dead animal recovery; and/or being out of regular work may have posed a risk to worker respiratory health through psychological stress mechanisms. OBJECTIVE: To evaluate the association between six potentially stressful oil spill experiences and lung function among OSRC workers 1-3 years following the Deepwater Horizon disaster, while controlling for primary oil spill inhalation hazards and other potential confounders...
October 11, 2018: Environment International
https://www.readbyqxmd.com/read/30317076/determination-of-caffeoylquinic-acid-derivatives-in-azolla-imbricata-by-chitosan-based-matrix-solid-phase-dispersion-coupled-with-hplc-pda
#10
Weilin Wu, Wenqi Qian, Haitian Hao, Yun Kang, Yaqin Wang, Yuanrong Deng, Chengjie Ni, Jianming Huang, Weiyu Weng
Five main components of Azolla imbricata were isolated and identified as caffeoylquinic acid derivatives, four of which were isolated from this species for the first time. The five compounds exhibited strong antioxidant activities and were used as chemical markers for quantitative analysis. A chitosan-based matrix solid-phase dispersion (MSPD) extraction coupled with high-performance liquid chromatography (HPLC) was developed for the determination of the five analytes in A. imbricata. The optimal conditions for the chitosan-based MSPD extraction were as follows: low viscosity chitosan HL-1 as the dispersant, sample-to-dispersant mass ratio of 1:1, 10 mL of a methanol-sulfuric acid aqueous solution (0...
October 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30317075/is-the-hematocrit-still-an-issue-in-quantitative-dried-blood-spot-analysis
#11
REVIEW
Sofie Velghe, Lisa Delahaye, Christophe P Stove
Hematocrit-related issues remain a major barrier for (regulatory) acceptance of the classical dried blood spot (DBS) analysis in the bioanalytical and clinical field. Lately, many attempts to cope with these issues have been made. Throughout this review, an overview is provided on new strategies that try to cope with this hematocrit effect (going from avoiding to minimizing), on methods estimating a DBS volume, and on methods estimating or measuring the hematocrit of a DBS. Although many successful strategies have been put forward, a combination of different technologies still provides the most complete solution...
October 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30317057/hyperbolic-subtraction-method-determination-of-the-concentration-of-an-analyte-in-the-presence-of-an-unknown-interferent-via-spectral-data
#12
Francisco Bosch-Reig, Antonio Doménech-Carbó
A theoretical model to determine the concentration of an analyte in the presence of unknown interferents using spectral-type data is described. The method involves absorbance measurements at three wavelengths and the calculation of specific absorbances yielding a hyperbolic relationship between absorbance ratios and analyte concentrations. The concentration of the analyte of known spectrum can be determined in the presence of an interferent or mixture of interferents of unknown concentration(s) and spectra can be determined combining data for different sets of wavelengths...
October 4, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/30316866/abnormal-amygdala-resting-state-functional-connectivity-in-adults-and-adolescents-with-major-depressive-disorder-a-comparative-meta-analysis
#13
Shi Tang, Lu Lu, Lianqing Zhang, Xinyu Hu, Xuan Bu, Hailong Li, Xiaoxiao Hu, Yingxue Gao, Zirui Zeng, Qiyong Gong, Xiaoqi Huang
BACKGROUND: Although dysfunction of amygdala-related circuits is centrally implicated in major depressive disorder (MDD), little is known about how this dysfunction differs between adult and adolescent MDD patients. METHODS: Voxel-wise meta-analyses of abnormal amygdala resting-state functional connectivity (rsFC) were conducted in adult and adolescent groups separately, followed by a quantitative meta-analytic comparison of the two groups. FINDINGS: Nineteen studies that included 665 MDD patients (392 adults and 273 adolescents) and 546 controls (341 adults and 205 adolescents) were identified in the current study...
October 10, 2018: EBioMedicine
https://www.readbyqxmd.com/read/30316794/analytical-aspects-of-printed-oral-dosage-forms
#14
Magnus Edinger, Jette Jacobsen, Daniel Bar-Shalom, Jukka Rantanen, Natalja Genina
Printing technologies, both 2D and 3D, have gained considerable interest during the last years for manufacturing of personalized dosage forms, tailored to each patient. Here we review the research work on 2D printing techniques, mainly inkjet printing, for manufacturing of film-based oral dosage forms. We describe the different printing techniques and give an overview of film-based oral dosage forms produced using them. The main part of the review focuses on the non-destructive analytical methods used for evaluation of qualitative aspects of printed dosage forms, e...
October 11, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/30316741/assortative-mating-on-complex-traits-revisited-double-first-cousins-and-the-x-chromosome
#15
Loic Yengo, Peter M Visscher
Mate choice through direct assortment on heritable traits, assortative mating (AM), is predicted in theory to inflate the genetic variance in a population and the correlation between relatives. Here, we revisit the theory of AM, first established in the landmark 1918 paper from RA Fisher, and provide new theory and analytical results. In particular, we shed light on inconsistencies in the literature regarding the correlation between double first cousins under AM and provide a solution. We derive new theory for AM due to X-chromosome loci...
October 11, 2018: Theoretical Population Biology
https://www.readbyqxmd.com/read/30316721/cortisol-and-alpha-amylase-assessment-in-psychotherapeutic-intervention-studies-a-systematic-review
#16
REVIEW
Sebastian Laufer, Sinha Engel, Christine Knaevelsrud, Sarah Schumacher
Dysregulations of the hypothalamus-pituitary-adrenal (HPA) axis and the autonomic nervous system (ANS) as parts of the stress response system have been associated with development and maintenance of various mental disorders. It has been suggested that these alterations might normalize in the course of psychotherapy. We conducted a comprehensive review of psychotherapeutic intervention effects on HPA axis and ANS regulation in adult samples with mental disorders. We searched four databases for psychotherapeutic intervention studies with mentally ill patient samples, assessing cortisol and/or α-amylase before and after treatment...
October 11, 2018: Neuroscience and Biobehavioral Reviews
https://www.readbyqxmd.com/read/30316629/epidemiologic-analyses-with-error-prone-exposures-review-of-current-practice-and-recommendations
#17
REVIEW
Pamela A Shaw, Veronika Deffner, Ruth H Keogh, Janet A Tooze, Kevin W Dodd, Helmut Küchenhoff, Victor Kipnis, Laurence S Freedman
PURPOSE: Variables in observational studies are commonly subject to measurement error, but the impact of such errors is frequently ignored. As part of the STRengthening Analytical Thinking for Observational Studies Initiative, a task group on measurement error and misclassification seeks to describe the current practice for acknowledging and addressing measurement error. METHODS: Task group on measurement error and misclassification conducted a literature survey of four types of research studies that are typically impacted by exposure measurement error: (1) dietary intake cohort studies, (2) dietary intake population surveys, (3) physical activity cohort studies, and (4) air pollution cohort studies...
September 18, 2018: Annals of Epidemiology
https://www.readbyqxmd.com/read/30316615/chromatographic-determination-and-in-situ-cell-imaging-of-thiol-compounds-based-on-a-fluorigenic-probe
#18
Liwei Cao, Qiuxia Liang, Tian Wei, Yihui Shi, Tao Deng, Jianxin Meng
This study combines a high-performance liquid chromatography-fluorescent detection method (HPLC-FLD) with in-situ cell imaging for the sensitive analysis of glutathione (GSH), cysteine (Cys) and homocysteine (Hcys), using BODIPY®507/545 IA as a labeling reagent. The analytical potential of BODIPY®507/545 IA in cell imaging was deeply explored, concerning fluorescent response, selectivity, cell-permeability, biotoxicity and so on. It is demonstrated that BODIPY®507/545 IA has good biocompatibility and the fluorescence intensity is enhanced remarkably after reacting with thiols...
September 27, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30316614/two-dimensional-insertable-separation-tool-twist-for-flow-confinement-in-spatial-separations
#19
Theodora Adamopoulou, Sander Deridder, Gert Desmet, Peter J Schoenmakers
Spatial comprehensive two-dimensional liquid chromatography (x LC×x LC) may be an efficient approach to achieve high peak capacities in relatively short analysis times, thanks to parallel second-dimension separations [1,2]. A key issue to reach the potential of x LC×x LC is to achieve adequate flow control and confinement of the analytes to the desired regions, i.e. confinement in the first-dimension direction and subsequently homogeneous flow in the second dimension. To achieve these goals we propose the TWIST concept (TWo-dimensional Insertable Separation Tool), a modular device that includes an internal first-dimension (1 D) part that is cylindrical and rotatable...
September 26, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/30316608/genetic-variation-in-serum-protein-pattern-and-blood-%C3%AE-hydroxybutyrate-and-their-relationships-with-udder-health-traits-protein-profile-and-cheese-making-properties-in-holstein-cows
#20
Alessio Cecchinato, Tania Bobbo, Pamela L Ruegg, Luigi Gallo, Giovanni Bittante, Sara Pegolo
The aim of this study was to investigate in Holstein cows the genetic basis of blood serum metabolites [i.e., total protein, albumin, globulin, albumin:globulin ratio (A:G), and blood β-hydroxybutyrate (BHB)], a set of milk phenotypes related to udder health, milk quality technological characteristics, and genetic relationships among them. Samples of milk were collected from 498 Holstein cows belonging to 28 herds. All animal welfare and milk phenotypes were assessed using standard analytical methodology. A set of Bayesian univariate and bivariate animal models was implemented via Gibbs sampling, and statistical inference was based on the marginal posterior distributions of parameters of concern...
October 10, 2018: Journal of Dairy Science
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