Alexandre Glémain, Mélanie Néel, Antoine Néel, Gwennan André-Grégoire, Julie Gavard, Bernard Martinet, Rozenn Le Bloas, Kevin Riquin, Mohamed Hamidou, Fadi Fakhouri, Sarah Bruneau
The critical role of neutrophils in pathological inflammation, notably in various autoimmune disorders, is currently the focus of renewed interest. Here, we demonstrate for the first time that activation of neutrophils with various inflammatory stimuli induces the release of extracellular vesicles (EVs) that are internalized by endothelial cells (ECs), thus leading to the transfer of miR-223, miR-142-3p and miR-451 and subsequent endothelial damage. Indeed, while miR-223 has little effect on EC responses, we show that the induced expression of miR-142-3p and miR-451 in ECs results in profound cell damage, especially in inflammatory conditions, characterized by a dramatic increase in cell apoptosis, impaired angiogenic repair responses, and the induction of IL-6, IL-8, CXCL10 and CXCL11 expression...
May 2022: Journal of Autoimmunity