keyword
https://read.qxmd.com/read/35727842/host-clic4-expression-in-the-tumor-microenvironment-is-essential-for-breast-cancer-metastatic-competence
#21
JOURNAL ARTICLE
Vanesa C Sanchez, Howard H Yang, Alayna Craig-Lucas, Wendy Dubois, Brandi L Carofino, Justin Lack, Jennifer E Dwyer, R Mark Simpson, Christophe Cataisson, Max P Lee, Ji Luo, Kent W Hunter, Stuart H Yuspa
The TGF-β-regulated Chloride Intracellular Channel 4 (CLIC4) is an essential participant in the formation of breast cancer stroma. Here, we used data available from the TCGA and METABRIC datasets to show that CLIC4 expression was higher in breast cancers from younger women and those with early-stage metastatic disease. Elevated CLIC4 predicted poor outcome in breast cancer patients and was linked to the TGF-β pathway. However, these associations did not reveal the underlying biological contribution of CLIC4 to breast cancer progression...
June 2022: PLoS Genetics
https://read.qxmd.com/read/35397614/n6-methyladenosine-demethylase-fto-suppressed-prostate-cancer-progression-by-maintaining-clic4-mrna-stability
#22
JOURNAL ARTICLE
Libin Zou, Wenbin Chen, Xumin Zhou, Taowei Yang, Junqi Luo, Zining Long, Jun Wu, Daojun Lv, Xiangming Mao, Shengren Cen
The fat mass and obesity-associated protein (FTO) is an N6-Methyladenosine (m6A) demethylase, which has been revealed to play critical roles in tumorigenesis. However, its role in the development and progression of prostate cancer (PCa) remains poorly understood. Here, we aimed to investigate the function and clinical relevance of FTO in PCa. Our results demonstrated that FTO was notably downregulated in PCa tissues compared with the paired normal tissues. In addition, the decreased expression of FTO was correlated with poor prognosis of PCa...
April 9, 2022: Cell Death Discovery
https://read.qxmd.com/read/35378380/neutrophil-derived-extracellular-vesicles-induce-endothelial-inflammation-and-damage-through-the-transfer-of-mirnas
#23
JOURNAL ARTICLE
Alexandre Glémain, Mélanie Néel, Antoine Néel, Gwennan André-Grégoire, Julie Gavard, Bernard Martinet, Rozenn Le Bloas, Kevin Riquin, Mohamed Hamidou, Fadi Fakhouri, Sarah Bruneau
The critical role of neutrophils in pathological inflammation, notably in various autoimmune disorders, is currently the focus of renewed interest. Here, we demonstrate for the first time that activation of neutrophils with various inflammatory stimuli induces the release of extracellular vesicles (EVs) that are internalized by endothelial cells (ECs), thus leading to the transfer of miR-223, miR-142-3p and miR-451 and subsequent endothelial damage. Indeed, while miR-223 has little effect on EC responses, we show that the induced expression of miR-142-3p and miR-451 in ECs results in profound cell damage, especially in inflammatory conditions, characterized by a dramatic increase in cell apoptosis, impaired angiogenic repair responses, and the induction of IL-6, IL-8, CXCL10 and CXCL11 expression...
May 2022: Journal of Autoimmunity
https://read.qxmd.com/read/35081862/circular-rna_0033596-aggravates-endothelial-cell-injury-induced-by-oxidized-low-density-lipoprotein-via-microrna-217-5p-chloride-intracellular-channel-4-axis
#24
JOURNAL ARTICLE
Bai Jing, Zhou Hui
In recent years, the modulatory functions of some circular RNAs (circRNAs) in the pathogenesis of atherosclerosis (AS) have been reported. Nonetheless, the role of circular RNA_0033596 (circ_0033596) in AS and its mechanism remains unclarified. In this study, oxidized low-density lipoprotein (ox-LDL) was applied to treat human umbilical vein endothelial cells (HUVECs) to establish a cell model of endothelial cell injury. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were employed to detect the expression of circ_0033596, microRNA-217-5p (miR-217-5p), and chloride intracellular channel 4 (CLIC4) in HUVECs...
February 2022: Bioengineered
https://read.qxmd.com/read/35078024/characterization-of-chloride-intracellular-channel-4-in-the-regulation-of-human-trophoblast-function
#25
JOURNAL ARTICLE
Wei Zhou, Ellen Menkhorst, Evdokia Dimitriadis
INTRODUCTION: Proper placentation requires well controlled extravillous trophoblast cell (EVT) migration and invasion. Transforming growth factor β (TGFβ) signaling has been well characterized as negatively regulating EVT migration and invasion. CLIC4 is an enhancer of TGFβ signaling, however CLIC4's function in placentation and its association to placental TGFβ signaling is unknown. Here we aimed to investigate the role of CLIC4 on trophoblast cell function and its relationship to TGFβ signaling...
March 4, 2022: Placenta
https://read.qxmd.com/read/35071703/targeted-proteomic-analysis-dataset-of-archival-core-human-kidney-biopsies-to-investigate-the-biology-of-hypertensive-nephropathy
#26
JOURNAL ARTICLE
Georgios Barkas, Manousos Makridakis, Hariklia Gakiopoulou, Demetrios Vlahakos
Hypertensive nephropathy is the second most frequent cause of end-stage renal disease in western societies. In previous experiments in our laboratory with proteomic analysis of renal parenchyma of SHR hypertensive animals, we identified two molecules, namely SGLT2 and CLIC4, associated with the development of hypertension. Here, we apply the methodology of targeted proteomic analysis in kidney biopsies from patients with hypertensive nephropathy to study the role of SGLT2 and CLIC4 in the pathogenesis of the disease...
February 2022: Data in Brief
https://read.qxmd.com/read/35042858/retinal-pigment-epithelium-specific-clic4-mutant-is-a-mouse-model-of-dry-age-related-macular-degeneration
#27
JOURNAL ARTICLE
Jen-Zen Chuang, Nan Yang, Nobuyuki Nakajima, Wataru Otsu, Cheng Fu, Howard Hua Yang, Maxwell Ping Lee, Armaan Fazal Akbar, Tudor Constantin Badea, Ziqi Guo, Afnan Nuruzzaman, Kuo-Shun Hsu, Joshua L Dunaief, Ching-Hwa Sung
Age-related macular degeneration (AMD) is the leading cause of blindness among the elderly. Dry AMD has unclear etiology and no treatment. Lipid-rich drusen are the hallmark of dry AMD. An AMD mouse model and insights into drusenogenesis are keys to better understanding of this disease. Chloride intracellular channel 4 (CLIC4) is a pleomorphic protein regulating diverse biological functions. Here we show that retinal pigment epithelium (RPE)-specific Clic4 knockout mice exhibit a full spectrum of functional and pathological hallmarks of dry AMD...
January 18, 2022: Nature Communications
https://read.qxmd.com/read/35007834/comparative-kinetic-analysis-of-ascorbate-vitamin-c-recycling-dehydroascorbate-reductases-from-plants-and-humans
#28
COMPARATIVE STUDY
Bhaba Krishna Das, Amit Kumar, Sreeshma Nellootil Sreekumar, Kannapiran Ponraj, Kaustubh Gadave, Saravanan Kumar, V Mohan Murali Achary, Pratima Ray, Malireddy K Reddy, Arulandu Arockiasamy
Ascorbate is an important cellular antioxidant that gets readily oxidized to dehydroascorbate (DHA). Recycling of DHA is therefore paramount in the maintenance of cellular homeostasis and preventing oxidative stress. Dehydroascorbate reductases (DHARs), in conjunction with glutathione (GSH), carry out this vital process in eukaryotes, among which plant DHARs have garnered considerable attention. A detailed kinetic analysis of plant DHARs relative to their human counterparts is, however, lacking. Chloride intracellular channels (HsCLICs) are close homologs of plant DHARs, recently demonstrated to share their enzymatic activity...
February 5, 2022: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/34839533/chloride-intracellular-channel-4-clic4-expression-profile-in-the-mouse-medial-prefrontal-cortex-and-its-regulation-by-ethanol
#29
JOURNAL ARTICLE
James W Bogenpohl, Rory M Weston, Taylor N Foreman, Kaitlyn E Kitchen, Michael F Miles
BACKGROUND: Chloride intracellular channel 4 (CLIC4) is a multifunctional metamorphic protein for which a growing body of evidence supports a major role in the brain's molecular and behavioral responses to ethanol (EtOH). Although key to understanding the functional biology underlying this role, little is known about the cellular and subcellular expression patterns of CLIC4 in brain and how they are affected by EtOH. METHODS: We used qRT-PCR to assess Clic4 mRNA expression in the medial prefrontal cortex (mPFC) of C57BL/6J mice in the absence and presence of acute EtOH exposure...
January 2022: Alcoholism, Clinical and Experimental Research
https://read.qxmd.com/read/34697147/detection-of-cells-displaying-high-expression-of-clic4-in-tumor-tissue-of-patients-with-colorectal-cancer
#30
JOURNAL ARTICLE
Reo Yokoyama, Atsuhito Kubota, Hiroyuki Kojima, Takuji Tanaka, Michihiro Mutoh, Masaru Terasaki
BACKGROUND/AIM: Chloride intracellular channel 4 (CLIC4) is associated with the progression of colorectal cancer (CRC). However, quantitative differences in CLIC4 expression in epithelial and stromal cells of normal mucosal tissue (NT), cancer adjacent to normal colorectal mucosal tissue (NAT), and CRC tissue remain unclear. MATERIALS AND METHODS: We investigated the number of CLIC4 high-expressing (CLIC4high ) cells in colorectal tissue of CRC patients and healthy individuals...
November 2021: In Vivo
https://read.qxmd.com/read/34440131/dna-hypermethylation-involves-in-the-down-regulation-of-chloride-intracellular-channel-4-clic4-induced-by-photodynamic-therapy
#31
JOURNAL ARTICLE
Pei-Chi Chiang, Pei-Tzu Li, Ming-Jen Lee, Chin-Tin Chen
The altered expression of chloride intracellular channel 4 (CLIC4) was reported to correlate with tumor progression. Previously, we have shown that the reduced cellular invasion induced by photodynamic therapy (PDT) is associated with suppression of CLIC4 expression in PDT-treated cells. Herein, we attempted to decipher the regulatory mechanisms involved in PDT-mediated CLIC4 suppression in A375 and MDA-MB-231 cells in vitro. We found that PDT can increase the expression and enzymatic activity of DNA methyltransferase 1 (DNMT1)...
July 31, 2021: Biomedicines
https://read.qxmd.com/read/34363182/dexmedetomidine-alleviates-lipopolysaccharide-induced-hippocampal-neuronal-apoptosis-via-inhibiting-the-p38-mapk-c-myc-clic4-signaling-pathway-in-rats
#32
JOURNAL ARTICLE
Yongping Chen, Lin Li, Jiuyan Zhang, Hailin Cui, Jiucheng Wang, Chuqiao Wang, Mingxian Shi, Honggang Fan
Dexmedetomidine (DEX) has multiple biological effects. Here, we investigated the neuroprotective role and molecular mechanism of DEX against lipopolysaccharide (LPS)-induced hippocampal neuronal apoptosis. Sprague Dawley rats were intraperitoneally injected with LPS (10 mg/kg) and/or DEX (30 µg/kg). We found that DEX improved LPS-induced alterations of hippocampal microstructure (necrosis and neuronal loss in the CA1 and CA3 regions) and ultrastructure (mitochondrial damage). DEX also attenuated LPS-induced inflammation and hippocampal apoptosis by inhibiting the increase of interleukin-1β, interleukin-6, interleukin-18, and tumor necrosis factor-α levels and downregulating the expression of mitochondrial apoptosis pathway-related proteins...
August 7, 2021: Molecular Neurobiology
https://read.qxmd.com/read/34269702/circ-usp9x-inhibition-reduces-oxidized-low-density-lipoprotein-induced-endothelial-cell-injury-via-the-microrna-599-chloride-intracellular-channel-4-axis
#33
JOURNAL ARTICLE
Huaiyu Peng, Jihu Sun, Yi Li, Ye Zhang, Yongjin Zhong
Atherosclerosis (AS) is the common pathological basis of cardiovascular disease. Circular RNA circ-USP9X (hsa_circ_0090231) has been discovered to be upregulated in oxidized low-density lipoprotein (ox-LDL)-induced human umbilical vein endothelial cells (HUVECs), but the role of circ-USP9X in ox-LDL-induced endothelial cell injury is indistinct. The purpose of the research was to investigate the role and regulatory mechanism of circ-USP9X in ox-LDL--induced endothelial cell injury. Expression of circ-USP9X was examined by quantitative real-time polymerase chain reaction...
October 1, 2021: Journal of Cardiovascular Pharmacology
https://read.qxmd.com/read/34228751/transcriptome-analysis-of-chloride-intracellular-channel-knockdown-in-drosophila-identifies-oxidation-reduction-function-as-possible-mechanism-of-altered-sensitivity-to-ethanol-sedation
#34
JOURNAL ARTICLE
Rory M Weston, Rebecca E Schmitt, Mike Grotewiel, Michael F Miles
Chloride intracellular channels (CLICs) are a unique family of evolutionarily conserved metamorphic proteins, switching between stable conformations based on redox conditions. CLICs have been implicated in a wide variety biological processes including ion channel activity, apoptosis, membrane trafficking, and enzymatic oxidoreductase activity. Understanding the molecular mechanisms by which CLICs engage in these activities is an area of active research. Here, the sole Drosophila melanogaster ortholog, Clic, was targeted for RNAi knockdown to identify genes and biological processes associated with Clic expression...
2021: PloS One
https://read.qxmd.com/read/34191361/retraction
#35
(no author information available yet)
Retraction: "SiRNA-Mediated Down-Regulation of CLIC4 Gene Inhibits Cell Proliferation and Accelerates Cell Apoptosis of Mouse Liver Cancer Hca-F and Hca-P Cells," by Qiu-Yun Yu, Xin-Feng Zhou, Qing Xia, Jia Shen, Jia Yan, Jiu-Ting Zhu, Xiang Li, and Ming Shu, J Cell Biochem. 2018; 659-668: The above article, published online on 21 June 2017 in Wiley Online Library (https://doi.org/10.1002/jcb.26229), has been retracted by agreement between the authors, the journal's Editor in Chief, Prof. Dr. Christian Behl, and Wiley Periodicals LLC...
November 2021: Journal of Cellular Biochemistry
https://read.qxmd.com/read/33879602/clic1-and-clic4-mediate-endothelial-s1p-receptor-signaling-to-facilitate-rac1-and-rhoa-activity-and-function
#36
JOURNAL ARTICLE
De Yu Mao, Matthew L Kleinjan, Irina Jilishitz, Bhairavi Swaminathan, Hideru Obinata, Yulia A Komarova, Kayla J Bayless, Timothy Hla, Jan K Kitajewski
Chloride intracellular channels 1 (CLIC1) and 4 (CLIC4) are expressed in endothelial cells and regulate angiogenic behaviors in vitro, and the expression of Clic4 is important for vascular development and function in mice. Here, we found that CLIC1 and CLIC4 in endothelial cells regulate critical G protein-coupled receptor (GPCR) pathways associated with vascular development and disease. In cultured endothelial cells, we found that CLIC1 and CLIC4 transiently translocated to the plasma membrane in response to sphingosine 1-phosphate (S1P)...
April 20, 2021: Science Signaling
https://read.qxmd.com/read/33822672/long-non-coding-rna-tdrg1-facilitates-cell-proliferation-migration-and-invasion-in-breast-cancer-via-targeting-mir-214-5p-clic4-axis
#37
JOURNAL ARTICLE
Yanrong Lv, Ke Dong, Haidong Gao
Accumulated studies have revealed the critical role of long non-coding RNAs (lncRNAs) in the carcinogenesis and progression of various cancers. LncRNA TDRG1 has been reported to exhibit oncogenic potential in some cancers. However, its underlying mechanism regulating breast cancer (BC) remains obscure. QRT-PCR was used to measure the relative expression of mRNAs, and western blot was used to detect protein expression levels. CCK8 and CFSE assays were utilized to testify cell proliferation ability. Flow cytometry assay was used for cell apoptosis ability investigation...
March 4, 2021: Cancer Biology & Therapy
https://read.qxmd.com/read/33796129/integrated-transcriptomic-analysis-of-the-mirna-mrna-interaction-network-in-thin-endometrium
#38
JOURNAL ARTICLE
Lu Zong, Shengxia Zheng, Ye Meng, Wenjuan Tang, Daojing Li, Zhenyun Wang, Xianhong Tong, Bo Xu
Although the thin endometrium (TE) has been widely recognized as a critical factor in implantation failure, the contribution of miRNA-mRNA regulatory network to the development of disease etiology remains to be further elucidated. This study performed an integrative analysis of the miRNA-mRNA expression profiles in the thin and adjacent normal endometrium of eight patients with intrauterine adhesion to construct the transcriptomic regulatory networks. A total of 1,093 differentially expressed genes (DEGs) and 72 differentially expressed miRNAs (DEMs) were identified in the thin adhesive endometrium of the TE group compared with the control adjacent normal endometrial cells...
2021: Frontiers in Genetics
https://read.qxmd.com/read/33703973/effects-of-clic4-on-fucoxanthinol-induced-apoptosis-in-human-colorectal-cancer-cells
#39
JOURNAL ARTICLE
Reo Yokoyama, Hiroyuki Kojima, Rie Takai, Tohru Ohta, Hayato Maeda, Kazuo Miyashita, Michihiro Mutoh, Masaru Terasaki
Fucoxanthin is a marine xanthophyll found in edible brown algae, and a metabolite, fucoxanthinol (FxOH), possesses a potent apoptosis inducing effect in many cancer cells. Chloride intracellular channel 4 (CLIC4) is a member of the CLIC family that plays an important role in cancer development and apoptosis. However, the role of CLIC4 in FxOH-induced apoptosis is not well understood. In this study, we investigated whether CLIC4 affects the apoptotic properties of FxOH in human colorectal cancer (CRC) cells under FxOH treatment...
2021: Nutrition and Cancer
https://read.qxmd.com/read/33439448/astragaloside-iv-alleviates-atherosclerosis-through-targeting-circ_0000231-mir-135a-5p-clic4-axis-in-as-cell-model-in-vitro
#40
JOURNAL ARTICLE
Xiao Shao, Zhaozheng Liu, Shanshan Liu, Na Lin, Yue Deng
Non-coding RNAs (ncRNAs) have shown to act as crucial mediators in atherosclerosis (AS) development. The purpose of our study was to explore the role of Astragaloside IV (ASV) and circular RNA_0000231 (circ_0000231) in AS using AS cell model. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry were used to analyze cell viability and apoptosis. Migration ability was assessed by transwell migration assay and wound healing assay. The inflammatory response was evaluated via enzyme-linked immunosorbent assay (ELISA)...
January 13, 2021: Molecular and Cellular Biochemistry
keyword
keyword
165389
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.