Alice Wedler, Nadine Bley, Markus Glaß, Simon Müller, Alexander Rausch, Marcell Lederer, Julia Urbainski, Laura Schian, Kingsley-Benjamin Obika, Theresa Simon, Lara Meret Peters, Claudia Misiak, Tommy Fuchs, Marcel Köhn, Roland Jacob, Tony Gutschner, Christian Ihling, Andrea Sinz, Stefan Hüttelmaier
The RAVER1 protein serves as a co-factor in guiding the polypyrimidine tract-binding protein (PTBP)-dependent control of alternative splicing (AS). Whether RAVER1 solely acts in concert with PTBPs and how it affects cancer cell fate remained elusive. Here, we provide the first comprehensive investigation of RAVER1-controlled AS in cancer cell models. This reveals a pro-oncogenic role of RAVER1 in modulating tumor growth and epithelial-mesenchymal-transition (EMT). Splicing analyses and protein-association studies indicate that RAVER1 guides AS in association with other splicing regulators, including PTBPs and SRSFs...
February 1, 2024: Nucleic Acids Research