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ROS generating agent

Pietro Minuz, Alessandra Meneguzzi, Laura Fumagalli, Maurizio Degan, Stefano Calabria, Roberta Ferraro, Marco Ricci, Dino Veneri, Giorgio Berton
The thromboxane (TX) A2 elicits TP-dependent different platelet responses. Low amounts activate Src kinases and the Rho-Rho kinase pathway independently of integrin αIIb β3 and ADP secretion and synergize with epinephrine to induce aggregation. Aim of the present study was to investigate the role Src kinases and the interplay with calcium signals in reactive oxygen species (ROS) generation in the activatory pathways engaged by TXA2 in human platelets. All the experiments were performed in vitro or ex vivo ...
2018: Frontiers in Pharmacology
Hong Cheng, Rong-Rong Zheng, Gui-Ling Fan, Jing-Hao Fan, Lin-Ping Zhao, Xue-Yan Jiang, Bin Yang, Xi-Yong Yu, Shi-Ying Li, Xian-Zheng Zhang
Mitochondria and cell membrane play important roles in maintaining cellular activity and stability. Here, a single-agent self-delivery chimeric peptide based nanoparticle (designated as M-ChiP) was developed for mitochondria and plasma membrane dual-targeted photodynamic tumor therapy. Without additional carrier, M-ChiP possessed high drug loading efficacy as well as the excellent ability of producing reactive oxygen species (ROS). Moreover, the dual-targeting property facilitated the effective subcellular localization of photosensitizer protoporphyrin IX (PpIX) to generate ROS in situ for enhanced photodynamic therapy (PDT)...
October 5, 2018: Biomaterials
Vanesa de Oliveira Pateis, Lívia Bracht, Lorena Dos Santos Castro, Gabriela Bueno Franco Salla, Jurandir Fernando Comar, Angela Valderrama Parizotto, Rosane Marina Peralta, Adelar Bracht
A study of the effects of butylated hydroxyanisole (BHA) on the hepatic metabolism was conducted with emphasis on parameters linked to energy metabolism and mitochondrial reactive oxygen species production. The experimental systems were the isolated perfused rat liver and isolated mitochondria. It was found that BHA inhibits biosynthetic pathways (gluconeogenesis) and ammonia detoxification, which are dependent on ATP generated within the mitochondria. Conversely, the compound stimulated glycolysis and fructolysis, which are compensatory phenomena for an inhibited mitochondrial ATP generation...
October 9, 2018: Toxicology Letters
Khatereh Khorsandi, Reza Hosseinzadeh, Fedora Khatibi Shahidi
Photodynamic treatment is a minimally invasive and clinically approved procedure for eliminating selected malignant cells with activation of a photosensitizer agent at a specific light. Little is known, however, about the phototoxic properties of curcumin, as a natural phenolic compound, against different types of cancers. It is generally accepted that cellular damage occurs during photo treatment. There is a limitation in using of curcumin as a drug due to its low solubility, but nanoparticles such as anionic nanoclays or layered double hydroxide (LDH) could overcome it...
October 9, 2018: Journal of Cellular Biochemistry
Kandukuri Sowmya, Dev Mala, Mohd Iqbal Bhat, Naveen Kumar, Rajesh Kumar Bajaj, Suman Kapila, Rajeev Kapila
Inflammation and oxidative stress are closely linked patho-physiological processes which occur concurrently in many diseased conditions. Recently, interdependence between these two processes explains the antioxidant paradox associated with failure to select appropriate agents required for prevention of diseases known to be induced by oxidative stress. Present study established the overlapping anti-inflammatory and anti-oxidative potential along with bio-accessibility of milk casein derived tripeptide (LLY)...
September 13, 2018: Journal of Nutritional Biochemistry
Norihiko Narita, Yumi Ito, Tetsuji Takabayashi, Masayuki Okamoto, Yoshimasa Imoto, Kazuhiro Ogi, Takahiro Tokunaga, Hideki Matsumoto, Shigeharu Fujieda
INTRODUCTION: Cisplatin is used as a standard chemotherapeutic agent for head and neck cancer treatment. However, some head and neck cancers have cisplatin resistance, leading to difficulty in treatment and poor prognosis. Overcoming cisplatin resistance remains an important strategy to improve prognoses for head and neck cancer patients. OBJECTIVE: Elucidation of the mechanisms underlying cisplatin resistance can suggest novel targets to enhance the anticancer effects of cisplatin for treating head and neck cancers...
October 9, 2018: International Journal of Hyperthermia
Wei Chen, Jingyun Luan, Gang Wei, Xuyao Zhang, Jiajun Fan, Wenjing Zai, Shaofei Wang, Yichen Wang, Yanxu Liang, Yanyang Nan, Chuzhen Yin, Yubin Li, Ming-Lin Liu, Dianwen Ju
Hepatocellular injury is the pathological hallmark of hepatitis and a crucial driver for the progression of liver diseases, while the treatment options are commonly restricted. Interleukin-22 (IL-22) has attracted special attention as a potent survival factor for hepatocytes that both prevents and repairs the injury of hepatocytes through activation of STAT3 signaling pathway. We hypothesized that the ability to generate potent expression of IL-22 locally for the treatment of severe hepatocellular injury in hepatitis was a promising strategy to enhance efficacy and overcome off-target effects...
October 3, 2018: Biomaterials
Licong Yang, Wenjun Wang, Jiguang Chen, Na Wang, Guodong Zheng
Deposition of amyloid-β (Aβ) aggregates and formation of neurotoxic reactive oxygen species (ROS) are significant pathological signatures of Alzheimer's disease (AD). Resveratrol (Res) is an antioxidant with the potential to treat AD. However, the bioavailability and solubility of Res is very low and it cannot entirely inhibit Cu2+ -induced Aβ42 aggregation at low concentration. Herein, we combine the unique Aβ absorption property of selenium nanoparticles with the natural antioxidant agent Res to form Res@SeNPs...
September 7, 2018: Journal of Biomedical Materials Research. Part A
Chen-Li Cheng, Wei-Ting Chao, Yu-Hsuan Li, Yen-Chuan Ou, Shian-Shiang Wang, Kun-Yuan Chiu, Sheau-Yun Yuan
Escin (β-escin) is used as traditional folk medicine. The anti-tumour effects of escin have been demonstrated in vitro in certain cell lines, but its effect on bladder cancer has not been well investigated. In this study, the apoptotic activity of escin dissolved in dimethyl sulfoxide (DMSO) in bladder cancer cells and normal peripheral blood mononuclear cells (PBMC) and SV-HUC1 cells (controls) was determined. Cell cytotoxicity was assessed using the MTT assay. Cell cycle, Reactive oxygen species (ROS) generation, annexin V-FITC staining (for detecting early apoptosis), and changes in mitochondrial membrane potential were evaluated using flow cytometry...
October 1, 2018: European Journal of Pharmacology
Rengarajan Baskaran, Junghan Lee, Su-Geun Yang
Background: Photodynamic therapy (PDT) is photo-treatment of malignant or benign diseases using photosensitizing agents, light, and oxygen which generates cytotoxic reactive oxygens and induces tumour regressions. Several photodynamic treatments have been extensively studied and the photosensitizers (PS) are key to their biological efficacy, while laser and oxygen allow to appropriate and flexible delivery for treatment of diseases. Introduction: In presence of oxygen and the specific light triggering, PS is activated from its ground state into an excited singlet state, generates reactive oxygen species (ROS) and induces apoptosis of cancer tissues...
2018: Biomaterials Research
Nalinee Kanth Kadiyala, Badal Kumar Mandal, Shivendu Ranjan, Nandita Dasgupta
The development of novel functionalized reduced graphene oxide nanocomposite materials keeping in mind environmental and health perspectives via green approaches is currently gaining enormous research interest in the field of nanoscience and nanotechnology. Herein, we report a bio-inspired green synthesis approach for gold nanoparticles decorated reduced graphene oxide nanocomposite in which Syzygium cumini seed extract (SCSE) is applied as natural reducing and stabilizing agent for the simultaneous reduction of chloroauric acid and graphene oxide (GO)...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Huan He, Yang Qiao, Zeyu Zhang, Zelong Wu, Dan Liu, Zhangping Liao, Dong Yin, Ming He
Vitamin C, an excellent reducing agent, aids in increasing absorbable ferrous iron in iron deficiency anemia. As an efficient antioxidant, it is still unknown whether vitamin C exerts protective effects against liver damage caused by iron excess and whether mitochondria are the target effectors of the above effects. In this study, 48 mice were randomly divided into a control group, iron-overload group, TAU-treated + iron-overload group and vitamin C-treated + iron-overload group with 12 mice per group. The mice were fed 4 months on pellet diets supplemented with iron in the form of ferrocene...
October 17, 2018: Food & Function
Ming-De Li, Nai-Kei Wong, Jia Xiao, Ruixue Zhu, Liangliang Wu, Shengyao Dai, Feng Chen, Guanheng Huang, Xiaoyu Bai, Margarita R Geraskina, Arthur H Winter, Xuebo Chen, Yingxia Liu, Wei-Hai Fang, Dan Yang, David Lee Phillips
Development of versatile, chemically tunable photocages for photoactivated chemotherapy (PACT) represents an excellent opportunity to address the technical drawbacks of conventional photodynamic therapy (PDT) whose oxygen-dependent nature renders it inadequate in certain therapy contexts such as hypoxic tumors. As an alternative to PDT, oxygen free mechanisms to generate cytotoxic reactive oxygen species (ROS) by visible light cleavable photocages are in demand. Here, we report the detailed mechanisms by which the small molecule blebbistatin acts as a one-photon blue light-gated or two-photon near infrared light-gated photocage to directly release a hydroxyl radical (•OH) in the absence of oxygen...
September 30, 2018: Journal of the American Chemical Society
Yoo-Jung Lee, Song-Yi Beak, Inho Choi, Jung-Suk Sung
Alcohol-induced liver disease progresses due to increased reactive oxygen species (ROS) and cellular lipid peroxidation. Quercetin is a flavonoid with strong antioxidant and hepatoprotective effects. We investigated whether 3'- O -methyl quercetin (3'MQ) and quercetin-3- O -glucuronide (Q3GA), two metabolites of quercetin, have protective effects against ethanol-induced hepatotoxicity. Cell viability was increased by quercetin, 3'MQ, and Q3GA in HepG2 hepatocarcinoma cells exposed to ethanol. Our results show that this effect was mediated by diminished ROS generation, decreased lipid peroxidation and up-regulation of antioxidant capacity, including glutathione, superoxide dismutase and catalase...
June 2018: Food Science and Biotechnology
Guo Zu, Tingting Zhou, Ningwei Che, Xiangwen Zhang
BACKGROUND/AIMS: Ischemia-reperfusion (I/R) adversely affects the intestinal mucosa. The major mechanisms of I/R are the generation of reactive oxygen species (ROS) and apoptosis. Salvianolic acid A (SalA) is suggested to be an effective antioxidative and antiapoptotic agent in numerous pathological injuries. The present study investigated the protective role of SalA in I/R of the intestine. METHODS: Adult male Sprague-Dawley rats were subjected to intestinal I/R injury in vivo...
2018: Cellular Physiology and Biochemistry
C D Barrett, A T Hsu, C D Ellson, B Y Miyazawa, Y-W Kong, J D Greenwood, S Dhara, M D Neal, J L Sperry, M S Park, M J Cohen, B S Zuckerbraun, M B Yaffe
Polymorphonuclear (PMN) leucocytes participate in acute inflammatory pathologies such as acute respiratory distress syndrome (ARDS) following traumatic injury and shock, which also activates the coagulation system systemically. Trauma can prime the PMN nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex for an enhanced respiratory burst, but the relative role of various priming agents in this process remains incompletely understood. We therefore set out to identify mediators of PMN priming during coagulation and trauma-shock and determine whether PMN reactive oxygen species (ROS) generated in this manner could influence organ injury and coagulation...
October 2018: Clinical and Experimental Immunology
Philippe Hantson
BACKGROUND: Dilated cardiomyopathy is a frequent disease responsible for 40-50% of cases of heart failure. Idiopathic cardiomyopathy is a primary disorder often related to familial/genetic predisposition. Before the diagnosis of idiopathic cardiomyopathy is made, clinicians must not only rule out viral and immune causes, but also toxic causes such as drugs, environmental agents, illicit substances and natural toxins. OBJECTIVE: The objective of this review is to present recent data on the mechanisms underlying toxic cardiomyopathy...
September 27, 2018: Clinical Toxicology
Fan Jiang, Hong-S Xing, Wei-Y Chen, Jie Du, Yue-L Ruan, An-Y Lin, Chi-Z Zhou
Itraconazole is an FDA-approved antifungal agent, which has been reported to possess promising anticancer activities in recent years. This study investigates the antiproliferative effects of itraconazole on pancreatic cancer cells and the molecular mechanism of its apoptosis-inducing effects. In this study, our results showed that itraconazole inhibited the growth of pancreatic cancer cells in vitro, and it also significantly inhibited the tumor growth of CFPAC-1 xenografts in vivo. Itraconazole induced apoptosis through ROS generation and mitochondrial membrane depolarization...
September 27, 2018: Journal of Cellular Biochemistry
Asmaa A Alsousi, Orisa J Igwe
Rheumatoid arthritis (RA) is a chronic autoimmune systemic inflammatory disease that is characterized by synovial inflammation and bone erosion. We have investigated the mechanism(s) by which essential trace metals may initiate and propagate inflammatory phenotypes in synovial fibroblasts. We used HIG-82, rabbit fibroblast-like synovial cells (FLS), as a model system for potentially initiating RA through oxidative stress. We used potassium peroxychromate (PPC, Cr+5 ), ferrous chloride (FeCl2, Fe+2 ), and cuprous chloride (CuCl, Cu+ ) trace metal agents as exogenous pro-oxidants...
November 2018: Biochimica et biophysica acta. Molecular basis of disease
Subodh Kumar, Sanjeev Kumar Sharma, Gaurav Kaushik, Pramod Kumar Avti, Satish Kumar Pandey, Phulen Sarma, Bikash Medhi, Krishan Lal Khanduja
Cigarette smoke is responsible for multiple disorders and causes almost 10 million annual deaths globally but underlying mechanisms are still underexplored. Continuous exposure of Cigarette smoke condensate (CSC) leads to cytosolic phospholipase A2 (cPLA2 ) mediated high free radicals where cPLA2 s seems to play crucial role in generated various patho-physiological conditions such as chronic inflammation, oxidative stress and cancer. In this view, we assessed the therapeutic potential of arachidonyl trifluromethyl ketone (ATK), a cPLA2 inhibitor, via pharmacological inhibition of most expressible CSC-induced cPLA2 group IVA in type-I and type-II alveolar epithelial cells...
September 22, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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