Erika Calvano Küchler, Raquel Fernanda Gerlach, Arthur S Cunha, Lucas A Ramazzotto, Paula Porto Spada, Paulo Nelson-Filho, Junia Ramos, Giovana Daniela Pecharki, Fernando Barbosa, Alexandre Rezende Vieira, Paula Cristina Trevilatto, João Armando Brancher
Homeostasis between salivary calcium and phosphorus is important for maintaining oral health. The aim of this study was to evaluate if polymorphisms in ESR1 (Estrogen Receptor Alpha), ESR2 (Estrogen Receptor Beta) and miRNA17 (microRNA17) are associated with calcium and phosphorus levels in saliva. Saliva from 276 12-year-old children were collected by masticatory stimulation and calcium and phosphorus levels were determined by Mass Spectrometry. Genomic DNA was extracted from remaining saliva and genetic polymorphisms in ESR1 (rs12154178, rs1884051, rs9340799 and rs2234693), in ESR2 (rs4986938 and rs1256049) and in miRNA17 (rs4284505) were genotyped using TaqMan chemistry and a real-time PCR equipment...
September 2020: Brazilian Dental Journal