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Immunophenotypage

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https://www.readbyqxmd.com/read/24103271/-primary-culture-and-multiple-differentiation-potency-of-mesenchymal-stem-cells-from-human-umbilical-cord
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Yi Xin, Na Li, Yimin Huang, Wei Cui, Sa Liu, Xiufang Xu, Zhaoguang Zhang
Objective To establish a reliable method of isolation, culture and characterization of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and study its multiple differentiation potency. Methods HUCMSCs were isolated and cultured using Trypsin-type II collagen and hyaluronidase digestion method and tissue explant culture method, respectively. The cell growth of hUCMSCs was observed under an inverted microscope. Cell viability rate of the different passages was evaluated by trypan blue staining. The proliferation profile of hUCMSCs was analyzed by growth curve and MTT assay...
October 2013: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
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