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UVB fibroblast aging

Furong Fan, Yufei Li, Yi Liu, Li Shao, Juehua Yu, Ziwei Li
Co-culture methods are widely used in tissue engineering to drive tissue formation with the direct or indirect interaction of multiple cell types. Klotho is a novel biomarker involved in aging. In this study, we evaluated the protective effects of klotho overexpressed adipose-derived stem cells (ADSCs) against ultraviolet radiation B (UVB)-induced photoaging in co-cultured human skin fibroblasts (HSF2 cell line). Furthermore, the involvement of P38 mitogen-activated protein kinase (MAPK) signaling was investigated...
December 2018: Molecular Medicine Reports
Shuanghai Hu, Jinhua Huang, Shiyao Pei, Yujie Ouyang, Yufang Ding, Ling Jiang, Jianyun Lu, Liyang Kang, Lihua Huang, Hong Xiang, Rong Xiao, Qinghai Zeng, Jing Chen
Ultraviolet (UV)-induced pigmentation is very common in clinical practice, but the current treatments are rarely effective, accompanied by some side effects. Ganoderma lucidum polysaccharide (GLP) is a natural antioxidant with no toxic side effects, which can antagonize UVB-induced fibroblast photo aging. The study aims to explore the role of GLP in inhibiting UVB-induced melanogenesis and its possible mechanism. The expression of melanogenesis genes such as microphthalmia-associated transcription factor (MITF), tyrosine (TYR), tyrosinase related protein 1 (TYRP1), tyrosinase related protein 2 (TYRP2), ras-related protein Rab-27A (Rab27A), and Myosin shows an upward trend after exposure of B16F10 and PIG1 cells to UVB irradiation, but GLP can downregulate the expression of genes related to UVB-induced melanogenesis...
October 26, 2018: Journal of Cellular Physiology
Genji Imokawa
Exposure of human skin to ultraviolet (UV) radiation causes significant damage to that tissue. The effects of UV on the skin mainly include acute inflammation (erythema/edema) and abnormal keratinization wherein prostaglandin E2 (produced by cyclooxygenase-2), interleukin-8 and transglutaminase 1 (a major regulatory factor of keratinization), play pivotal roles. Later phases of UV-induced skin reactions include hyperpigmentation, wrinkle formation and carcinogenesis, the former two being associated with the UVB-induced production and/or secretion of endothelin-1, stem cell factor and granulocyte macrophage colony stimulatory factor by keratinocytes in the epidermis...
October 19, 2018: Photochemistry and Photobiology
Weijie Wen, Jianwen Chen, Liugang Ding, Xia Luo, Xueping Zheng, Qi Dai, Qianqian Gu, Cui Liu, Ming Liang, Xiaolei Guo, Peiqing Liu, Min Li
BACKGROUND: Astragalus membranaceus is a fundamental herb in Traditional Chinese Medicine and has attracted significant attention due to its anti-inflammatory, and longevity effects. However, its anti-photoaging property remains to be defined. Autophagy plays important roles in regulating cell homeostasis and aging processes. Whether regulation of autophagy could be an efficient way for anti-photoaging is still unclear. OBJECTIVE: To investigate the effects and the possible mechanism of astragaloside on anti-photoaging in UVB-induced photoaging cell model...
September 14, 2018: Archives of Biochemistry and Biophysics
Eunson Hwang, Pei Lin, Hien T T Ngo, Tae-Hoo Yi
Syzygium aromaticum L., commonly named clove, is widely used in the food industry due to its antioxidant and antibacterial capabilities. However, little information is available regarding its role in resisting skin photoaging. This study investigated 50% ethanol extract of Syzygium aromaticum L. (SA) and eugenol (EO) for anti-aging effects in UVB-irradiated normal human dermal fibroblasts (NHDFs) and hairless mice. In vitro, SA and EO suppressed matrix metalloproteinase-1, 3 (MMP-1 and MMP-3) secretion as well as the activator protein 1 (AP-1) phosphorylation...
September 19, 2018: Food & Function
Yun-A Park, Sung Ryul Lee, Jin Woo Lee, Hyun Jung Koo, Seon-A Jang, Seung-Won Yun, Hyun Ju Kim, Jeong Suk Woo, Myung Rye Park, Se Chan Kang, Youn Kyu Kim, Eun-Hwa Sohn
Angelica tenuissima root has historically been used as a traditional medicine in Korea. Previous studies have identified the anti-melanogenic effects of the extract of A. tenuissima root fermented by Aspergillus oryzae (FAT). This study investigated the protective effects of FAT against ultraviolet light B exposure (UVB; 30 mJ/cm2 ) in HaCaT (human keratinocyte) or Hs68 (human foreskin fibroblast) skin cells. FAT treatment was able to stimulate wound healing rate at the basal condition. FAT also favored the maintenance and/or improvement of extracellular matrix impairment caused by UVB irradiation through: 1) upregulation of procollagen Type-1 synthesis and secretion; 2) suppression of MMP-1 and elastase expression...
August 28, 2018: Journal of Microbiology and Biotechnology
Suwen Liu, Lu You, Yanxue Zhao, Xuedong Chang
Ultraviolet (UV) B radiation can cause skin aging by increasing matrix metalloproteinase (MMP) production and collagen degradation, leading to the formation of wrinkles. This study investigated whether hawthorn polyphenol extract (HPE) protects against UVB-induced skin photoaging using HaCaT human keratinocytes, normal human dermal fibroblasts (HDFs), and mice. Analysis of the phenol composition of HPE by high-performance liquid chromatography-mass spectrometry showed that chlorogenic acid (13.5%), procyanidin B2 (19...
August 15, 2018: Journal of Agricultural and Food Chemistry
Mingwu Deng, Dong Li, Yichen Zhang, Guangdong Zhou, Wei Liu, Yilin Cao, Wenjie Zhang
Ultraviolet B (UVB) radiation induces the production of reactive oxygen species (ROS), resulting in the aging of dermal fibroblasts. Crocin, a bioactive constituent of Crocus sativus, possesses anti‑oxidation effects. The purpose of the present study was to evaluate the protective effect of crocin on UVB‑induced dermal fibroblast photoaging. Human dermal fibroblasts were isolated and cultured with different concentrations of crocin prior to and following exposure to UVB irradiation. The senescent phenotypes of cells were evaluated, including cell proliferation, cell cycle, senescence‑associated β‑galactosidase (SA‑β‑gal) expression, intracellular ROS, expression of antioxidant protein glutathione peroxidase 1 (GPX‑1) and extracellular matrix protein collagen type 1 (Col‑1)...
August 2018: Molecular Medicine Reports
Myeongsik Oh, Jinhee Lee, Yu Jin Kim, Won Jong Rhee, Ju Hyun Park
Stem cells and their paracrine factors have emerged as a resource for regenerative medicine. Many studies have shown the beneficial effects of paracrine factors secreted from adult stem cells, such as exosomes, on skin aging. However, to date, few reports have demonstrated the use of exosomes derived from human pluripotent stem cells for the treatment of skin aging. In this study, we collected exosomes from the conditioned medium of human induced pluripotent stem cells (iPSCs) and investigated the effect on aged human dermal fibroblasts (HDFs)...
June 9, 2018: International Journal of Molecular Sciences
Dengke Qin, Runjian Ren, Chuanlong Jia, Yongzhou Lu, Qingjian Yang, Liang Chen, Xinyuan Wu, Jingjing Zhu, Yu Guo, Ping Yang, Yiqun Zhou, Ningwen Zhu, Bo Bi, Tianyi Liu
BACKGROUND/AIMS: Ultraviolet B (UVB) irradiation alters multiple molecular pathways in the skin, thereby inducing skin photoaging. Murine dermal fibroblasts (MDFs) were subjected to a series of 4 sub-cytotoxic UVB doses (120 mJ/cm2), resulting in changes in cell shape, DNA damage, cell cycle arrest, extracellular matrix variations, reactive oxygen species (ROS) generation, and alterations in major intracellular antioxidant and cellular autophagy levels. Rapamycin (RAPA) is a new macrolide immunosuppressive agent that is primarily used in oncology, cardiology, and transplantation medicine and has been found to extend the lifespan of genetically heterogeneous mice...
2018: Cellular Physiology and Biochemistry
Ramesh Kumar Santhanam, Sharida Fakurazi, Syahida Ahmad, Faridah Abas, Intan Safinar Ismail, Yaya Rukayadi, Muhammad Tayyab Akhtar, Khozirah Shaari
The antiphoto aging property of Zanthoxylum rhetsa obtained from Pangkor Island, Malaysia, was evaluated. Solvent fractions of different polarity obtained from the methanolic extract of the bark material were initially tested for anticollagenase and antielastase activities. The ethyl acetate fraction showed bioactivity against the protease enzymes. Hence, it was subjected to further purification via column chromatography, to yield a major constituent, hesperidin. Subsequently, the ethyl acetate fraction and hesperidin were tested for their effects against UVB-induced cytotoxicity and expressions of inflammatory cytokines (IL-6, IL-1β, and TNF-α), NF-κB, and MMPs (MMP1, 3, and 9) in human dermal fibroblasts (HDF)...
August 2018: Phytotherapy Research: PTR
Marie Toutfaire, Elise Dumortier, Antoine Fattaccioli, Martine Van Steenbrugge, Charlotte M Proby, Florence Debacq-Chainiaux
Cutaneous Squamous Cell Carcinoma (cSCC) is the second most common type of non-melanoma skin cancer in white-skinned populations. cSCC is associated with sun exposure and aging, which is concomitant with an accumulation of senescent cells in the skin. The involvement of senescent cells in carcinogenesis has been highlighted in several cancer types and an interaction between cSCC cells and senescent cells is proposed, but still little explored. Tumor-associated effects are mostly attributed to the senescence-associated secretory phenotype (SASP)...
May 2018: International Journal of Biochemistry & Cell Biology
Hong-Il Kim, Yong-Un Jeong, Jong-Hyeon Kim, Young-Jin Park
Citrus fruits contain various types of flavonoids with powerful anti-aging and photoprotective effects on the skin, and have thus been attracting attention as potential, efficacious skincare agents. Here, we aimed to investigate the chemical composition of Citrus unshiu and its protective effects on photoaging. We isolated and identified a bioactive compound, 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF), from C. unshiu peels using ethanol extraction and hexane fractionation. HMF inhibited collagenase activity and increased type I procollagen content in UV-induced human dermal fibroblast neonatal (HDFn) cells...
February 22, 2018: International Journal of Molecular Sciences
Inhyuk Yeo, Yong-Jik Lee, Kyeongseop Song, Hyeon-Su Jin, Jae-Eun Lee, Dajeong Kim, Dong-Woo Lee, Nam Joo Kang
Bioactive peptides contribute to various cellular processes including improved skin physiology. Hence, bioactive keratins have attracted considerable attention as active cosmetic ingredients for skin health. Here, we obtained low molecular weight (LMW) keratins from native chicken feathers by anaerobic digestion with an extremely thermophilic bacterium Fervidobacterium islandicum AW-1, followed by stepwise fractionation through ultrafiltration. To assess the effects of the feather keratins on skin health, we performed in vitro and ex vivo assays to investigate their inhibitory effects on matrix metalloproteinases (MMPs)...
April 10, 2018: Journal of Biotechnology
Eunson Hwang, Hien T T Ngo, Seul A Seo, Bom Park, Mengyang Zhang, Tae-Hoo Yi
BACKGROUND: Alchemilla mollis (lady's mantle) is a common ingredient in skin care products. However, the protective mechanism of A. mollis against skin problems has not been elucidated. PURPOSE: This study was to investigate the effects of A. mollis ethanolic extract (AM) on UVB-irradiated normal human dermal fibroblasts (NHDF) and hairless mice. METHODS: The in vitro anti-photoaging effect of AM was performed in NHDFs. The antioxidant activities were assessed through DPPH, ABTS, and reactive oxygen species (ROS) assays...
January 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Lu Li, Eunson Hwang, Hien T T Ngo, Pei Lin, Wei Gao, Ying Liu, Tae-Hoo Yi
Cherry blossoms have attracted attention as an ingredient with potential for use in skincare products. However, no skin photoaging-related research has been performed with this plant. In this study, cherry blossom extract (CBE) at 1, 10 and 100 μg mL-1 was investigated for its skin antiphotoaging effects in UVB-irradiated normal human dermal fibroblasts (NHDF) cells in vitro. Our results showed that CBE markedly increased type-I procollagen during UVB exposure via two pathways. Firstly, transcription activator protein-1 expression and MAP kinases were downregulated, consequently reducing the production of matrix metalloproteinase (MMP)-1 and MMP-3...
July 2018: Photochemistry and Photobiology
Mengyang Zhang, Eunson Hwang, Pei Lin, Wei Gao, Hien T T Ngo, Tae-Hoo Yi
Prunella vulgaris L., a well-known traditional Chinese herbal medicine, has anti-inflammatory and antioxidant activities. In the present study, the underlying molecular mechanisms of the protective effect of P. vulgaris extract (PVE) were investigated in UVB-irradiated normal human dermal fibroblasts (NHDFs). The mRNA expression of matrix metalloproteinases (MMPs), procollagen type I, and cytokines, such as interleukin-6 (IL-6) and tumor necrosis factor (TNF-α), was determined by reverse transcription-polymerase chain reaction...
August 2018: Rejuvenation Research
Yo Han Hong, Donghyun Kim, Gibaeg Nam, Sulgi Yoo, Sang Yun Han, Seong-Gu Jeong, Eunji Kim, Deok Jeong, Keejung Yoon, Sunggyu Kim, Junseong Park, Jae Youl Cho
Background: BIOGF1K, a compound-K-rich fraction, has been shown to display anti-inflammatory activity. Although Panax ginseng is widely used for the prevention of photoaging events induced by UVB irradiation, the effect of BIOGF1K on photoaging has not yet been examined. In this study, we investigated the effects of BIOGF1K on UVB-induced photoaging events. Methods: We analyzed the ability of BIOGF1K to prevent UVB-induced apoptosis, enhance matrix metalloproteinase (MMP) expression, upregulate anti-inflammatory activity, reduce sirtuin 1 expression, and melanin production using reverse transcription-polymerase chain reaction, melanin content assay, tyrosinase assay, and flow cytometry...
January 2018: Journal of Ginseng Research
Seung-Hee Nam, Jeongjin Park, Woojin Jun, Doman Kim, Jin-A Ko, A M Abd El-Aty, Jin Young Choi, Do-Ik Kim, Kwang-Yeol Yang
Gallic acid glycoside was enzymatically synthesized by using dextransucrase and sucrose from gallic acid. After purification by butanol partitioning and preparative HPLC, gallic acid glucoside was detected at m/z 355 (C13 , H16 , O10 , Na)+ by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The yield of gallic acid glucoside was found to be 35.7% (114 mM) by response surface methodology using a reaction mixture of 319 mM gallic acid, 355 mM sucrose, and 930 mU/mL dextransucrase...
December 22, 2017: AMB Express
Chao Ji, Bo Yang, Shu-Ying Huang, Jin-Wen Huang, Bo Cheng
The role of UVB in skin photo damages has been widely reported. Overexposure to UVB will induce severe DNA damages in epidermal cells and cause most cytotoxic symptoms. In the present study, we tested the potential activity of salubrinal, a selective inhibitor of Eukaryotic Initiation Factor 2 (eIF2) -alpha phosphatase, against UV-induced skin cell damages. We first exposed human fibroblasts to UVB radiation and evaluated the cytosolic Ca2+ level as well as the induction of ER stress. We found that UVB radiation induced the depletion of ER Ca2+ and increased the expression of ER stress marker including phosphorylated PERK, CHOP, and phosphorylated IRE1α...
December 2, 2017: Biochemical and Biophysical Research Communications
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