Gabriele Beckers, Julia Strösser, Ulrich Hildebrandt, Jörn Kalinowski, Mike Farwick, Reinhard Krämer, Andreas Burkovski
AmtR, the master regulator of nitrogen control in Corynebacterium glutamicum, represses transcription of a number of genes during nitrogen surplus. Repression is released by an interaction of AmtR with signal transduction protein GlnK. As shown by pull-down assays and gel retardation experiments, only adenylylated GlnK, which is present in the cells during nitrogen limitation, is able to bind to AmtR. The AmtR regulon was characterized in this study by a combination of bioinformatics, transcriptome and proteome analyses...
October 2005: Molecular Microbiology