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Calcium imaging

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https://www.readbyqxmd.com/read/28640817/an-r-camp1-07-reporter-mouse-for-cell-type-specific-expression-of-a-sensitive-red-fluorescent-calcium-indicator
#1
Philipp Bethge, Stefano Carta, Dayra A Lorenzo, Ladan Egolf, Despoina Goniotaki, Linda Madisen, Fabian F Voigt, Jerry L Chen, Bernard Schneider, Masamichi Ohkura, Junichi Nakai, Hongkui Zeng, Adriano Aguzzi, Fritjof Helmchen
Genetically encoded calcium indicators (GECIs) enable imaging of in vivo brain cell activity with high sensitivity and specificity. In contrast to viral infection or in utero electroporation, indicator expression in transgenic reporter lines is induced noninvasively, reliably, and homogenously. Recently, Cre/tTA-dependent reporter mice were introduced, which provide high-level expression of green fluorescent GECIs in a cell-type-specific and inducible manner when crossed with Cre and tTA driver mice. Here, we generated and characterized the first red-shifted GECI reporter line of this type using R-CaMP1...
2017: PloS One
https://www.readbyqxmd.com/read/28640580/a-bio-inspired-alginate-gum-arabic-hydrogel-with-micro-nano-scale-structures-for-controlled-drug-release-in-chronic-wound-healing
#2
Mi Li, Haichang Li, Xiangguang Li, Hua Zhu, Zihui Xu, Lianqing Liu, Jianjie Ma, Mingjun Zhang
Bio-polymeric hydrogels have drawn increasingly research interests in biomaterials due to its tunable physical and chemical properties for both creating bioactive cellular micro-environment and serving as sustainable therapeutic reagents. Inspired by a naturally occurring hydrogel secreted from the carnivorous Sundew plant for trapping insects, here we have developed a bio-inspired hydrogel to deliver mitsugumin53 (MG53), an important protein in cell membrane repair, for chronic wound healing. Both chemical compositions and micro/nano morphological properties inherent from the natural Sundew hydrogel were mimicked using sodium alginate and gum arabic with calcium ion-mediated cross-linking...
June 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28635249/perfluorocarbon-gold-loading-for-non-invasive-in-vivo-assessment-of-bone-fillers-using-19f-magnetic-resonance-imaging-and-computed-tomography
#3
Simone Mastrogiacomo, Weiqiang Dou, Olga Koshkina, Otto C Boerman, John A Jansen, Arend Heerschap, Mangala Srinivas, X Frank Walboomers
Calcium phosphate cement (CPC) is used in bone repair because of its biocompatibility. However, high similarity between CPC and the natural osseous phase results in poor image contrast in most of the available in vivo imaging techniques such as computed tomography (CT) and magnetic resonance imaging (MRI). For accurate identification and localization during and after implantation in vivo, a composition with enhanced image contrast is needed. In this study, we labeled CPC with perfluoro-15-crown-5-ether-loaded (PFCE) poly(latic-co-glycolic acid) nanoparticles (hydrodynamic radius 100 nm) and gold nanoparticles (diameter 40 nm), as 19F MRI and CT contrast agents respectively...
June 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28631789/electrolyte-effect-in-induced-charge-electroosmosis
#4
Huicheng Feng, Yi Huang, Teck Neng Wong, Fei Duan
This paper reports an interesting phenomenon that the velocity increases with increasing sodium chloride (NaCl) concentration in induced charge electroosmosis (ICEO) around a conducting cylinder measured by microparticle image velocimetry (μPIV). It is different from the widely reported velocity decay with increasing electrolyte concentration in AC electroosmosis (ACEO) [M. Z. Bazant et al., MicroTAS, 2007, 2875-2878] and induced charge electrophoresis (ICEP) of Janus particles [S. Gangwal et al., Phys. Rev...
June 20, 2017: Soft Matter
https://www.readbyqxmd.com/read/28629730/synergistic-antinociceptive-effect-of-a-calcium-channel-blocker-and-a-trpv1-blocker-in-an-acute-pain-model-in-mice
#5
Manuella R Palhares, Juliana F Silva, Marcio Junior S Rezende, Duana C Santos, Cláudio A Silva-Junior, Márcia H Borges, Juliano Ferreira, Marcus V Gomez, Célio J Castro Junior
AIMS: Extensive evidence supports a role for voltage-gated calcium channels (VGCC) and TRPV1 receptors in pain transmission and modulation. We investigated the profile of analgesic interaction between Phα1β toxin (a VGCC blocker) and SB366791 (selective TRPV1 antagonist) in a model of acute pain induced by capsaicin. Changes in body temperature induced by combination regimens were also evaluated. MAIN METHODS: Isobolographic approach with a fixed dose-ratio of combined drugs was used to determine whether antinociceptive interaction of Phα1β and SB366791 are subadditive, additive or synergic...
June 16, 2017: Life Sciences
https://www.readbyqxmd.com/read/28629567/ct-coronary-calcium-scoring-with-tin-filtration-using-iterative-beam-hardening-calcium-correction-reconstruction
#6
Christian Tesche, Carlo N De Cecco, U Joseph Schoepf, Taylor M Duguay, Moritz H Albrecht, Domenico De Santis, Akos Varga-Szemes, Virginia W Lesslie, Ullrich Ebersberger, Richard R Bayer, Christian Canstein, Ellen Hoffmann, Thomas Allmendinger, John W Nance
OBJECTIVES: To investigate the diagnostic accuracy of CT coronary artery calcium scoring (CACS) with tin pre-filtration (Sn100kVp) using iterative beam-hardening correction (IBHC) calcium material reconstruction compared to the standard 120kVp acquisition. BACKGROUND: Third generation dual-source CT (DSCT) CACS with Sn100kVp acquisition allows significant dose reduction. However, the Sn100kVp spectrum is harder with lower contrast compared to 120kVp, resulting in lower calcium score values...
June 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/28627772/bactericidal-effect-of-calcium-oxide-scallop-shell-powder-against-pseudomonas-aeruginosa-biofilm-on-quail-egg-shell-stainless-steel-plastic-and-rubber
#7
Soo-Jin Jung, Shin Young Park, Seh Eun Kim, Ike Kang, Jiyong Park, Jungwon Lee, Chang-Min Kim, Myung-Sub Chung, Sang-Do Ha
The aim of this study was to evaluate the bactericidal effect of calcium oxide (CaO) against Pseudomonas aeruginosa biofilms on quail eggshells and major egg contacting surfaces (stainless steel, plastic, and rubber). The samples were subjected to CaO treatments (0%, 0.01%, 0.05%, 0.10%, 0.15%, 0.20%, 0.25%, and 0.30%) for 1 min. All the CaO treatments significantly reduced P. aeruginosa biofilms on all tested surfaces as compared to controls. In comparison of biofilm stability, the strongest and most resistant biofilm was formed on eggshell against the CaO treatment, followed by rubber, stainless steel, and plastic...
June 19, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28626925/stoichiometric-analysis-of-oligomeric-states-of-three-class-a-gpcrs-chemokine-cxcr4-dopamine-d2-and-prostaglandin-ep1-receptors-on-living-cells
#8
Kenichi Kawano, Tetsuya Yagi, Nozomu Fukada, Yoshiaki Yano, Katsumi Matsuzaki
G-protein-coupled receptors (GPCRs) form the largest family of transmembrane receptors, and their oligomerization has been suggested to be related to their functions. Despite extensive studies, their oligomeric states are highly controversial. One of the reasons is the overestimation of oligomerization by conventional methods. We recently established a stoichiometric analysis method for precisely determining the oligomeric state of membrane proteins on living cells with the combined use of the coiled-coil labeling method and a spectral imaging technique and showed that the prototypical class-A GPCR β2 -adrenergic receptor (β2 AR) did not form functional oligomers...
June 18, 2017: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/28626299/neutron-radiography-measurement-of-salt-solution-absorption-in-mortar
#9
Catherine L Lucero, Robert P Spragg, Dale P Bentz, Daniel S Hussey, David L Jacobson, W Jason Weiss
Some concrete pavements in the US have recently exhibited premature joint deterioration. It is hypothesized that one component of this damage can be attributed to a reaction that occurs when salt-laden water is absorbed in the concrete and reacts with the matrix. This study examines the absorption of CaCl2 solution in mortar via neutron imaging. Mortar specimens were prepared with water to cement ratios, (w/c), of 0.36, 0.42 and 0.50 by mass and exposed to chloride solutions with concentrations ranging from 0 % to 29...
January 1, 2017: ACI Mater J
https://www.readbyqxmd.com/read/28625916/er-stress-disturbs-sr-er-mitochondria-ca-2-transfer-implications-in-duchenne-muscular-dystrophy
#10
Marion Pauly, Claire Angebault-Prouteau, Haikel Dridi, Cécile Notarnicola, Valérie Scheuermann, Alain Lacampagne, Stefan Matecki, Jérémy Fauconnier
Besides its role in calcium (Ca(2+)) homeostasis, the sarco-endoplamic reticulum (SR/ER) controls protein folding and is tethered to mitochondria.Under pathophysiological conditions the unfolded protein response (UPR) is associated with disturbance in SR/ER-mitochondria crosstalk. Here, we investigated whether ER stress altered SR/ER-mitochondria links, Ca(2+) handling and muscle damage in WT (Wild Type) and mdx mice, the murine model of Duchenne Muscular Dystrophy (DMD). In WT mice, the SR/ER-mitochondria links were decreased in isolated FDB muscle fibers after injection of ER stress activator tunicamycin (TM)...
June 15, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28624464/complex-phenotypes-associated-with-stim1-mutations-in-both-coiled-coil-and-ef-hand-domains
#11
Elizabeth Harris, Umar Burki, Chiara Marini-Bettolo, Marcella Neri, Chiara Scotton, Judith Hudson, Marta Bertoli, Teresinha Evangelista, Bas Vroling, Tuomo Polvikoski, Mark Roberts, Ana Töpf, Kate Bushby, Daniel McArthur, Hanns Lochmüller, Alessandra Ferlini, Volker Straub, Rita Barresi
Dominant mutations in STIM1 are a cause of three allelic conditions: tubular aggregate myopathy, Stormorken syndrome (a complex phenotype including myopathy, hyposplenism, hypocalcaemia and bleeding diathesis), and a platelet dysfunction disorder, York platelet syndrome. Previous reports have suggested a genotype-phenotype correlation with mutations in the N-terminal EF-hand domain associated with tubular aggregate myopathy, and a common mutation at p.R304W in a coiled coil domain associated with Stormorken syndrome...
May 4, 2017: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/28624401/the-external-validity-of-prediction-models-for-the-diagnosis-of-obstructive-coronary-artery-disease-in-patients-with-stable-chest-pain-insights-from-the-promise-trial
#12
Tessa S S Genders, Adrian Coles, Udo Hoffmann, Manesh R Patel, Daniel B Mark, Kerry L Lee, Ewout W Steyerberg, M G Myriam Hunink, Pamela S Douglas
OBJECTIVES: This study sought to externally validate prediction models for the presence of obstructive coronary artery disease (CAD). BACKGROUND: A better assessment of the probability of CAD may improve the identification of patients who benefit from noninvasive testing. METHODS: Stable chest pain patients from the PROMISE (Prospective Multicenter Imaging Study for Evaluation of Chest Pain) trial with computed tomography angiography (CTA) or invasive coronary angiography (ICA) were included...
June 9, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28623807/hiv-tat-excites-d1-receptor-like-expressing-neurons-from-rat-nucleus-accumbens
#13
G Cristina Brailoiu, Elena Deliu, Jeffrey L Barr, Linda M Console-Bram, Alexandra M Ciuciu, Mary E Abood, Ellen M Unterwald, Eugen Brailoiu
BACKGROUND: HIV-1 infection and drug abuse are frequently co-morbid and their association greatly increases the severity of HIV-1-induced neuropathology. While nucleus accumbens (NAcc) function is severely perturbed by drugs of abuse, little is known about how HIV-1 infection affects NAcc. METHODS: We used calcium and voltage imaging to investigate the effect of HIV-1 trans-activator of transcription (Tat) on rat NAcc. Based on previous neuronal studies, we hypothesized that Tat modulates intracellular Ca(2+) homeostasis of NAcc neurons...
June 8, 2017: Drug and Alcohol Dependence
https://www.readbyqxmd.com/read/28623463/hypotonicity-induced-cell-swelling-activates-trpa1
#14
Fumitaka Fujita, Kunitoshi Uchida, Yasunori Takayama, Yoshiro Suzuki, Masayuki Takaishi, Makoto Tominaga
Hypotonic solutions can cause painful sensations in nasal and ocular mucosa through molecular mechanisms that are not entirely understood. We clarified the ability of human TRPA1 (hTRPA1) to respond to physical stimulus, and evaluated the response of hTRPA1 to cell swelling under hypotonic conditions. Using a Ca(2+)-imaging method, we found that modulation of AITC-induced hTRPA1 activity occurred under hypotonic conditions. Moreover, cell swelling in hypotonic conditions evoked single-channel activation of hTRPA1 in a cell-attached mode when the patch pipette was attached after cell swelling under hypotonic conditions, but not before swelling...
June 16, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/28622417/the-antimigraine-butterbur-ingredient-isopetasin-desensitises-peptidergic-nociceptors-via-the-transient-receptor-potential-ankyrin-1-channel
#15
Silvia Benemei, Francesco De Logu, Simone Li Puma, Ilaria Maddalena Marone, Elisabetta Coppi, Filippo Ugolini, Wolfgang Liedtke, Pollastro Federica, Giovanni Appendino, Pierangelo Geppetti, Serena Materazzi, Romina Nassini
BACKGROUND AND PURPOSE: The mechanism of the antimigraine action of butterbur [Petasites hybridus (L.) Gaertn.] is unknown. Here, we investigated the ability of isopetasin, a major butterbur constituent, to specifically target the transient receptor ankyrin 1 (TRPA1) channel and to affect functional responses relevant to migraine. EXPERIMENTAL APPROACH: Single cell calcium imaging and patch-clamp recordings in human and rodent TRPA1-expressing cells, neurogenic motor responses in isolated rat/mouse urinary bladder, release of calcitonin gene-related peptide (CGRP) from mouse spinal cord in vitro, and facial rubbing in mice and meningeal blood flow in rat were examined...
June 16, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28620120/ethyl-vanillin-activates-trpa1
#16
Shaw-Wen Wu, Daniel K Fowler, Forrest J Shaffer, Jonathon E M Lindberg, James Henry Peters
The non-selective cation channel TRPA1 is expressed in neurons of dorsal root ganglia and trigeminal ganglia and also in vagal afferent neurons that innervate the lungs and gastrointestinal tract. Many TRPA1 agonists are also reactive electrophilic compounds which form covalent adducts with TRPA1. Allyl isothiocyanate (AITC), the common reference agonist used to identify TRPA1, contains a reactive electrophilic group that covalently binds with cysteine residues of TRPA1 and confers a structural change on the channel...
June 15, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28619195/association-of-triglyceride-related-genetic-variants-with-mitral%C3%A2-annular%C3%A2-calcification
#17
Mehdi Afshar, Kevin Luk, Ron Do, Line Dufresne, David S Owens, Tamara B Harris, Gina M Peloso, Kathleen F Kerr, Quenna Wong, Albert V Smith, Mathew J Budoff, Jerome I Rotter, L Adrienne Cupples, Stephen S Rich, James C Engert, Vilmundur Gudnason, Christopher J O'Donnell, Wendy S Post, George Thanassoulis
BACKGROUND: Mitral annular calcium (MAC), commonly identified by cardiac imaging, is associated with cardiovascular events and predisposes to the development of clinically important mitral valve regurgitation and mitral valve stenosis. However, its biological determinants remain largely unknown. OBJECTIVES: The authors sought to evaluate whether a genetic predisposition to elevations in plasma lipids is associated with the presence of MAC. METHODS: The authors used 3 separate Mendelian randomization techniques to evaluate the associations of lipid genetic risk scores (GRS) with MAC in 3 large patient cohorts: the Framingham Health Study, MESA (Multiethnic European Study of Atherosclerosis), and the AGE-RS (Age, Gene/Environment Susceptibility-Reykjavik Study)...
June 20, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28619096/presenilins-regulate-synaptic-plasticity-and-mitochondrial-calcium-homeostasis-in-the-hippocampal-mossy-fiber-pathway
#18
Sang Hun Lee, David Lutz, Mohanad Mossalam, Vadim Y Bolshakov, Michael Frotscher, Jie Shen
BACKGROUND: Presenilins play a major role in the pathogenesis of Alzheimer's disease, in which the hippocampus is particularly vulnerable. Previous studies of Presenilin function in the synapse, however, focused exclusively on the hippocampal Schaffer collateral (SC) pathway. Whether Presenilins play similar or distinct roles in other hippocampal synapses is unknown. METHODS: To investigate the role of Presenilins at mossy fiber (MF) synapses we performed field and whole-cell electrophysiological recordings and Ca(2+) imaging using acute hippocampal slices of postnatal forebrain-restricted Presenilin conditional double knockout (PS cDKO) and control mice at 2 months of age...
June 15, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28619021/treatment-of-thoracolumbar-burst-fractures-by-short-segment-pedicle-screw-fixation-using-a-combination-of-two-additional-pedicle-screws-and-vertebroplasty-at-the-level-of-the-fracture-a-finite-element-analysis
#19
Jen-Chung Liao, Weng-Pin Chen, Hao Wang
BACKGROUND: Traditional one-above and one-below four-screw posterior short-segment instrumentation is used for unstable thoracolumbar burst fractures. However, this method has a high rate of implant failure and early loss of reduction. The purpose of this study was to use finite element (FE) analysis to determine the effect of treating thoracolumbar burst fractures by short-segment pedicle screw fixation using a combination of two additional pedicle screws and vertebroplasty at the level of the fracture...
June 15, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28616562/coronary-calcification-compromises-myocardial-perfusion-irrespective-of-luminal-stenosis
#20
Michael Y Henein, Tarek Bengrid, Rachel Nicoll, Ying Zhao, Bengt Johansson, Axel Schmermund
AIM: The aim of this study was to evaluate the relationship between coronary artery calcification (CAC) assessed by multi-detector computed tomography (MDCT) and myocardial perfusion assessed by cardiac magnetic resonance imaging (CMR) in a group of symptomatic patients. METHOD: Retrospective analysis of 120 patients (age 65.1 ± 8.9 years, 88 males) who presented with atypical chest pain to Bethanien Hospital, Frankfurt, Germany, between 2007 and 2010 and who underwent CAC scoring using MDCT, CMR, and conventional coronary angiography...
March 2017: IJC Heart & Vasculature
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