keyword
MENU ▼
Read by QxMD icon Read
search

Rip3

keyword
https://www.readbyqxmd.com/read/29352268/cylindromatosis-mediates-neuronal-cell-death-in-vitro-and-in-vivo
#1
Goutham K Ganjam, Nicole Angela Terpolilli, Sebastian Diemert, Ina Eisenbach, Lena Hoffmann, Christina Reuther, Christiane Herden, Joachim Roth, Nikolaus Plesnila, Carsten Culmsee
The tumor-suppressor cylindromatosis (CYLD) is a deubiquitinating enzyme and key regulator of cell proliferation and inflammation. A genome-wide siRNA screen linked CYLD to receptor interacting protein-1 (RIP1) kinase-mediated necroptosis; however, the exact mechanisms of CYLD-mediated cell death remain unknown. Therefore, we investigated the precise role of CYLD in models of neuronal cell death in vitro and evaluated whether CYLD deletion affects brain injury in vivo. In vitro, downregulation of CYLD increased RIP1 ubiquitination, prevented RIP1/RIP3 complex formation, and protected neuronal cells from oxidative death...
January 19, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29334122/inhibition-of-hsp90%C3%AE-protects-cultured-neurons-from-oxygen-glucose-deprivation-induced-necroptosis-by-decreasing-rip3-expression
#2
Zhen Wang, Li-Min Guo, Yong Wang, Hong-Kang Zhou, Shu-Chao Wang, Dan Chen, Ju-Fang Huang, Kun Xiong
Heat shock protein 90α (HSP90α) maintains cell stabilization and regulates cell death, respectively. Recent studies have shown that HSP90α is involved in receptor interacting protein 3 (RIP3)-mediated necroptosis in HT29 cells. It is known that oxygen and glucose deprivation (OGD) can induce necroptosis, which is regulated by RIP3 in neurons. However, it is still unclear whether HSP90α participates in the process of OGD-induced necroptosis in cultured neurons via the regulation of RIP3. Our study found that necroptosis occurs in primary cultured cortical neurons and PC-12 cells following exposure to OGD insult...
November 20, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29331417/down-regulation-of-rip3-potentiates-cisplatin-chemoresistance-by-triggering-hsp90-erk-pathway-mediated-dna-repair-in-esophageal-squamous-cell-carcinoma
#3
Yulin Sun, Linhui Zhai, Shouzhi Ma, Chengpu Zhang, Lina Zhao, Ning Li, Yang Xu, Tao Zhang, Zhimin Guo, Heng Zhang, Ping Xu, Xiaohang Zhao
Receptor interacting protein kinase 3 (RIP3) is a critical regulator of programmed necrotic cell death. Here, we observed that RIP3 was significantly down-regulated in esophageal cancer. And its remaining expression was associated with better response to chemotherapy and prolonged survival. Notably, re-expression of kinase-dead RIP3 also restored cisplatin sensitivity, suggesting that some roles of RIP3 beyond necroptosis may be involved in cisplatin-based chemosensitivity. To investigate the mechanisms, a large-scale quantitative proteomics study was performed after cisplatin treatment in RIP3-knockdown cells...
January 10, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29330116/tnf-%C3%AE-increases-staphylococcus-aureus-induced-death-of-human-alveolar-epithelial-cell-line-a549-associated-with-rip3-mediated-necroptosis
#4
Shun-Hang Wen, Luo-Na Lin, Hu-Jun Wu, Lu Yu, Li Lin, Li-Li Zhu, Hai-Yan Li, Hai-Lin Zhang, Chang-Chong Li
AIM: To explore the role of tumor necrosis factor-alpha (TNF-α) on Staphylococcus aureus-induced necroptosis in alveolar epithelial cells. MAIN METHODS: The A549 alveolar epithelial cell line was pretreated with small interfering RNA (siRNA) against receptor interacting protein-3 (RIP3) and then stimulated by S. aureus, where some cells were pretreated with TNF-α or TNF-α with anti-TNF-α antibody simultaneously. A549 cell death was assessed using lactate dehydrogenase (LDH) leakage and flow cytometry analyses...
January 9, 2018: Life Sciences
https://www.readbyqxmd.com/read/29313217/temporal-pattern-and-crosstalk-of-necroptosis-markers-with-autophagy-and-apoptosis-associated-proteins-in-ischemic-hippocampus
#5
Fari Ryan, Fariba Khodagholi, Leila Dargahi, Dariush Minai-Tehrani, Abolhassan Ahmadiani
Necroptosis, a novel type of programmed cell death, has been recently implicated as a possible mechanism for cerebral ischemia-reperfusion (I/R) injury. We herein studied time-dependent changes of necroptosis markers along with apoptosis- and autophagy-associated proteins in rat hippocampus at 1, 3, 6, 12, 24, and 48 h after global cerebral I/R injury. Furthermore, to determine the cross talk between autophagy and necroptosis, we examined the effects of pretreatment with bafilomycin-A1 (Baf-A1), as a late-stage autophagy inhibitor, on necroptosis...
January 8, 2018: Neurotoxicity Research
https://www.readbyqxmd.com/read/29299822/necrostatin-1-protects-against-paraquat-induced-cardiac-contractile-dysfunction-via-rip1-rip3-mlkl-dependent-necroptosis-pathway
#6
Liping Zhang, Qiming Feng, Teng Wang
Paraquat is a highly toxic prooxidant that triggers oxidative stress and multi-organ failure including that of the heart. To date, effective treatment of paraquat toxicity is still not established. Necroptosis, a newly discovered form of programmed cell death, was recently shown to be strongly associated with cardiovascular disease. Receptor interaction proteins 1 (RIP1), receptor interaction proteins 3 (RIP3), and mixed lineage kinase domain like (MLKL) are key proteins in the necroptosis pathway. Necrostatin-1 (Nec-1) is a specific inhibitor of necroptosis which acts by blocking the interaction between RIP1 and RIP3...
January 3, 2018: Cardiovascular Toxicology
https://www.readbyqxmd.com/read/29286079/exogenous-hydrogen-sulfide-protects-human-umbilical-vein-endothelial-cells-against-high-glucose%C3%A2-induced-injury-by-inhibiting-the-necroptosis-pathway
#7
Jiaqiong Lin, Meiji Chen, Donghong Liu, Ruixian Guo, Kai Lin, Haiou Deng, Ximei Zhi, Weijie Zhang, Jianqiang Feng, Wen Wu
Hyperglycemia is a key factor in the development of diabetic complications, including the processes of atherosclerosis. Receptor‑interacting protein 3 (RIP3), a mediator of necroptosis, is implicated in atherosclerosis development. Additionally, hydrogen sulfide (H2S) protects the vascular endothelium against hyperglycemia‑induced injury and attenuates atherosclerosis. On the basis of these findings, the present study aimed to confirm the hypothesis that necroptosis mediates high glucose (HG)‑induced injury in human umbilical vein endothelial cells (HUVECs), and that the inhibition of necroptosis contributes to the protective effect of exogenous H2S against this injury...
December 19, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29258580/apoptosis-and-necroptosis-of-mouse-hippocampal-and-parenchymal-astrocytes-microglia-and-neurons-caused-by-angiostrongylus-cantonensis-infection
#8
Zhang Mengying, Xu Yiyue, Pan Tong, Hu Yue, Yanin Limpanont, Huang Ping, Kamolnetr Okanurak, Wu Yanqi, Paron Dekumyoy, Zhou Hongli, Dorn Watthanakulpanich, Wu Zhongdao, Wang Zhi, Lv Zhiyue
BACKGROUND: Angiostrongylus cantonensis has been the only parasite among Angiostrongylidae to cause human central nervous system infection characterized by eosinophilic meningitis or meningoencephalitis. The mechanism of the extensive neurological impairments of hosts caused by A. cantonensis larvae remains unclear. The aim of the present study was to investigate apoptosis, necroptosis and autophagy in the brains of mice infected with A. cantonensis, which will be valuable for better understanding the pathogenesis of angiostrongyliasis cantonensis...
December 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29248830/mir-200a-5p-regulates-myocardial-necroptosis-induced-by-se-deficiency-via-targeting-rnf11
#9
Tianshu Yang, Changyu Cao, Jie Yang, Tianqi Liu, Xin Gen Lei, Ziwei Zhang, Shiwen Xu
Necroptosis has been discovered as a new paradigm of cell death and may play a key role in heart disease and selenium (Se) deficiency. Hence, we detected the specific microRNA (miRNA) in response to Se-deficient heart using microRNAome analysis. For high-throughput sequencing using Se-deficient chicken cardiac tissue, we selected miR-200a-5p and its target gene ring finger protein 11 (RNF11) based on differential expression in cardiac tissue and confirmed the relationship between miR-200a-5p and RNF11 by dual luciferase reporter assay and real-time quantitative PCR (qRT-PCR) in cardiomyocytes...
December 8, 2017: Redox Biology
https://www.readbyqxmd.com/read/29225581/protective-effect-of-unacylated-ghrelin-on-compression-induced-skeletal-muscle-injury-mediated-by-sirt1-signaling
#10
Felix N Ugwu, Angus P Yu, Thomas K Sin, Bjorn T Tam, Christopher W Lai, S C Wong, Parco M Siu
Unacylated ghrelin, the predominant form of circulating ghrelin, protects myotubes from cell death, which is a known attribute of pressure ulcers. In this study, we investigated whether unacylated ghrelin protects skeletal muscle from pressure-induced deep tissue injury by abolishing necroptosis and apoptosis signaling and whether these effects were mediated by SIRT1 pathway. Fifteen adult Sprague Dawley rats were assigned to receive saline or unacylated ghrelin with or without EX527 (a SIRT1 inhibitor). Animals underwent two 6-h compression cycles with 100 mmHg static pressure applied over the mid-tibialis region of the right limb whereas the left uncompressed limb served as the intra-animal control...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29212215/betanodavirus-b2-protein-triggers-apoptosis-and-necroptosis-in-lung-cancer-cells-that-suppresses-autophagy
#11
Hsuan-Wen Chiu, Yu-Chin Su, Jiann-Ruey Hong
The betanodavirus B2 protein targets the mitochondria and acts as a "death factor", but its effect on lung cancer cells is unknown. We examined the effect of the B2 protein on triggering apoptosis or necroptosis via P53-dependent and P53-independent pathways and increased in suppression of autophagy. The B2 protein targets the mitochondria of A549 (P53+/+) and H1299 (P53-/-) lung cancer cells due to a specific signal sequence (41RTFVISAHAA50). This triggers generation of reactive oxygen species within the mitochondria, and a minor stress response in A549 cells, but a strong stress response in H1299 cells...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29175336/simultaneous-flow-cytometric-immunophenotyping-of-necroptosis-apoptosis-and-rip1-dependent-apoptosis
#12
H L Lee, R Pike, M H A Chong, A Vossenkamper, G Warnes
Flow cytometry was been widely used to measure apoptosis for many decades but the researcher has no definitive way of determining other forms of cell death using this technology. The use of Western Blot technology has numerous drawbacks in that all the cells in the sample whether live, dead or maybe undergoing multiple discrete forms of cell death are analysed as one population. Flow cytometry given that it can analyse different sub-populations of cells within a sample would reveal the expression of cell death markers within these sub-populations rather than just give a single result from the entire population...
November 23, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29170425/tradd-mediates-the-tumor-necrosis-factor-induced-apoptosis-of-l929-cells-in-the-absence-of-rip3
#13
Xixi Chang, Lili Wang, Zicheng Wang, Shuai Wu, Xiaoming Zhu, Shiping Hu, Yu Wang, Jiyun Yu, Guozhu Chen
Receptor-interacting protein kinase 3 (RIP3) is a critical initiator in mediating necroptosis induced by tumor necrosis factor alpha (TNFα) in L929 cells, so knockdown of RIP3 inhibits TNFα-induced L929 cell necroptosis. However, RIP3 knockdown was shown to switch TNFα-induced necroptosis to apoptosis in L929 cells in other studies. Therefore, whether RIP3 knockdown blocks the TNFα-induced death of L929 cells is controversial. In this study, TNFα activated caspase pathway and induced cell death in RIP3 knockdown L929 cells, and the RIP3-independent cell death had been blocked by Z-VAD-FMK (pan-caspase inhibitor) or caspase 8 knockdown, demonstrating that RIP3 knockdown switched TNFα-induced necroptosis to caspase-dependent apoptosis...
November 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167229/embryonic-lethality-and-host-immunity-of-rela-deficient-mice-are-mediated-by-both-apoptosis-and-necroptosis
#14
Chengxian Xu, Xiaoxia Wu, Xixi Zhang, Qun Xie, Cunxian Fan, Haibing Zhang
In mammalian cells, signaling pathways triggered by TNF can be switched from NF-κB activation to apoptosis and/or necroptosis. The in vivo mechanisms underlying the mutual regulation of these three signaling pathways are poorly understood. In this article, we report that the embryonic lethality of RelA-deficient mice is partially prevented by the deletion of Rip3 or Mlkl, but it is fully rescued by the combined ablation of Fadd and Rip3 or Mlkl or by blocking RIP1 kinase activity (RIP1K45A). RelA-/-Fadd-/-Rip3-/- triple-knockout (TKO) and RelA-/-Rip1K45A/K45A mice displayed bacterial pneumonia leading to death ∼2 wk after birth...
January 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29140165/improved-total-body-irradiation-survival-by-delivery-of-two-radiation-mitigators-that-target-distinct-cell-death-pathways
#15
Justin Steinman, Michael Epperly, Wen Hou, John Willis, Hong Wang, Renee Fisher, Bing Liu, Ivet Bahar, Travis McCaw, Valerian Kagan, Hulya Bayir, Jian Yu, Peter Wipf, Song Li, M Saiful Huq, Joel S Greenberger
The acute lethality of total-body irradiation (TBI) involves damage to multiple organs, including bone marrow and intestine. Ionizing radiation mitigators that are effective when delivered 24 h or later after TBI include the anti-apoptotic drug, JP4-039 and the anti-necroptotic drug, necrostatin-1. In contrast to effective delivery of JP4-039 at 24 h after TBI, necrostatin-1 is most effective when delivery is delayed until 48 h, a time that correlates with the elevation of necroptosis-inducing inflammatory cytokines and necroptosis-induced serine phosphorylation of receptor-interacting serine/threonine-protein kinase-3 (RIP3) in tissues...
November 15, 2017: Radiation Research
https://www.readbyqxmd.com/read/29055012/type-i-interferon-enhances-necroptosis-of-salmonella-typhimurium-infected-macrophages-by-impairing-antioxidative-stress-responses
#16
Nina Judith Hos, Raja Ganesan, Saray Gutiérrez, Deniz Hos, Jennifer Klimek, Zeinab Abdullah, Martin Krönke, Nirmal Robinson
Salmonella enterica serovar Typhimurium exploits the host's type I interferon (IFN-I) response to induce receptor-interacting protein (RIP) kinase-mediated necroptosis in macrophages. However, the events that drive necroptosis execution downstream of IFN-I and RIP signaling remain elusive. In this study, we demonstrate that S Typhimurium infection causes IFN-I-mediated up-regulation of the mitochondrial phosphatase Pgam5 through RIP3. Pgam5 subsequently interacts with Nrf2, which sequesters Nrf2 in the cytosol, thereby repressing the transcription of Nrf2-dependent antioxidative genes...
October 20, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29037260/a-novel-protein-derived-from-lamprey-supraneural-body-tissue-with-efficient-cytocidal-actions-against-tumor-cells
#17
Yue Pang, Changzhi Li, Shiyue Wang, Wei Ba, Tao Yu, Guangying Pei, Dan Bi, Hongfang Liang, Xiong Pan, Ting Zhu, Meng Gou, Yinglun Han, Qingwei Li
BACKGROUND: In previous research, we found that cell secretion from the adult lamprey supraneural body tissues possesses cytocidal activity against tumor cells, but the protein with cytocidal activity was unidentified. METHODS: A novel lamprey immune protein (LIP) as defense molecule was first purified and identified in jawless vertebrates (cyclostomes) using hydroxyapatite column and Q Sepharose Fast Flow column. After LIP stimulation, morphological changes of tumor cells were analysed and measured whether in vivo or in vitro...
October 16, 2017: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/28993192/up-regulation-of-rip3-alleviates-cervical-cancer-progression-through-inducing-necroptosis
#18
Dong-Li Zhang, Gui-Xia Sun, Jun Tian, Hong-Xia Zhang
Receptor-interacting protein kinase-3 (RIP3 or RIPK3) is an important part of the cellular machinery, executing programmed necroptosis. However, the biological role and clinical significance of RIP3 in cervical cancer remains to be further elucidated. Here, we reported that RIP3 was expressed lowly in cervical cancer cell lines and clinical cervical tumor samples, along with the reduction of receptor-interacting protein 1 (RIP1) and p-mixed lineage kinase domain-like protein (MLKL). Further, we found that over-expressing RIP3 suppressed the proliferation and tumorigenicity of cervical cancer cells both in vitro and in vivo...
October 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28987363/extract-from-mulberry-morus-australis-leaf-decelerate-acetaminophen-induced-hepatic-inflammation-involving-downregulation-of-myeloid-differentiation-factor-88-myd88-signals
#19
Chi-Ting Horng, Zhi-Hong Liu, Yu-Ting Huang, Huei-Jane Lee, Chau-Jong Wang
Acetaminophen (APAP) induced inflammation and oxidative stress can cause cell death to induce liver damage. The antioxidative and anti-inflammatory effect of Mulberry (Morus australis) leaf extract (MLE) was shown in previous studies. In this study, we investigated the modulation of MLE on APAP induced inflammation and oxidative stress in rat liver injury or liver cancer cell (HepG2). Wistar rat was fed orally with MLE (0.5% or 1.0 %) for 1 week, and then, 900 mg/kg of APAP was injected intraperitoneally (i...
October 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28983589/honokiol-triggers-receptor%C3%A2-interacting-protein-kinase-3%C3%A2-mediated-cell-death-of-neuroblastoma-cells-by-upregulating-reactive-oxygen-species
#20
Jiao Zhang, Qiuliang Liu, Longyan Shi, Pan Qin, Qi Wang
Neuroblastoma is the most common form of childhood extracranial tumor and almost half of neuroblastoma cases occur in infants under two years old. Neuroblastoma accounts for ~6‑10% of childhood cancers and 15% of cancer‑associated childhood mortality. However, an effective treatment remains to be developed. Honokiol exhibits long‑lasting central muscle relaxation, anti‑inflammatory, antibacterial, antimicrobial, antiulcer, antioxidation, antiaging and antitumor effects. Honokiol has been previously demonstrated to kill neuroblastoma cells, however, the underlying mechanism of action remains unclear...
December 2017: Molecular Medicine Reports
keyword
keyword
16515
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"