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https://www.readbyqxmd.com/read/30336404/antimicrobial-susceptibility-pattern-of-aerobes-in-diabetic-foot-ulcers-in-a-south-indian-tertiary-care-hospital
#1
Sonal Sekhar M, Unnikrishnan M K, Gabriel Sunil Rodrigues, Navya Vyas, Chiranjay Mukhopadhyay
PURPOSE: Diabetic foot infections (DFIs) are major public health concerns. In the view of the ever increasing burden of multidrug-resistant (MDR) organisms, improving the use of antimicrobial agents (AMAs) is a national priority. Objective of the study was to determine antimicrobial susceptibility (AMS) pattern of aerobes in DFIs. METHODS: A cross-sectional study was conducted for a period of 3 years at Department of Surgery, Kasturba hospital, Manipal, India during which, 260 DFUs patients admitted in the general surgery wards were enrolled for the study...
July 11, 2018: Foot
https://www.readbyqxmd.com/read/30336286/novel-ca-2-independent-c-type-lectin-involved-in-immune-defense-of-the-razor-clam-sinonovacula-constricta
#2
Yuhong Shi, Xuelin Zhao, Zhenhui Wang, Yina Shao, Weiwei Zhang, Yongbo Bao, Chenghua Li
C-type lectins (CTLs) are important pattern recognition molecules that participate in bacterial binding and agglutination by specific recognition of carbohydrates from pathogens. In this study, a full-length cDNA of CTL was cloned from Sinonovacula constricta (designated ScCTL-2). ScCTL-2 has a length of 981 bp, a 5'-untranslated region (UTR) of 47 bp, a short 3'-UTR of 37 bp, and an open reading frame (ORF) of 894 bp, which encodes a polypeptide of 298 amino acid residues. The deduced amino acid of ScCTL-2 possesses a conserved carbohydrate-recognition domain (CRD) similar to that of C31 -E171 ...
October 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30336284/development-of-novel-antimicrobial-peptides-derived-from-anti-lipopolysaccharide-factor-of-the-swimming-crab-portunus-trituberculatus
#3
Bo-Hye Nam, Eun-Hee Park, Eun-Ha Shin, Young-Ok Kim, Dong-Gyun Kim, Hee Jeong Kong, Jung Youn Park, Jung-Kil Seo
Anti-lipopolysaccharide factors (ALFs) are a representative host defense protein in crustaceans. In this study, we successfully developed two novel antimicrobial peptides (AMPs), named crab-ALF2A and crab-ALF6A, which contain changes to the amino acid sequences of the lipopolysaccharide binding domain and signal peptide, respectively, of the ALF of the swimming crab Portunus trituberculatus. The crab-ALF2A peptide showed potent antimicrobial activity against the Gram-positive bacteria Bacillus cereus, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentration [MEC] 1...
October 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30336170/comparison-of-high-flow-nasal-cannula-versus-oxygen-face-mask-for-environmental-bacterial-contamination-in-critically-ill-pneumonia-patients-a-randomized-controlled-crossover-trial
#4
C C H Leung, G M Joynt, C D Gomersall, W T Wong, A Lee, L Ling, P K S Chan, P C W Lui, P C Y Tsoi, C M Ling, M Hui
Whereas high-flow nasal cannula use is gaining prevalence, its high gas flow raises concerns about aerosolization of infectious particles and spread of infection. This randomized controlled crossover non-inferiority trial (N = 20) evaluated the degree of environmental contamination by viable bacteria associated with the use of high-flow nasal cannula compared with conventional oxygen mask for critically ill patients with Gram-negative pneumonia. The results show that high-flow nasal cannula use was not associated with increased air or contact surface contamination by either Gram-negative bacteria or total bacteria, suggesting that additional infection control measures are not required...
October 15, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/30335609/metabolomic-fingerprint-of-mentha-rotundifolia-l-leaf-tissues-promotes-this-species-as-a-potential-candidate-for-sustainable-production-of-biologically-active-molecules
#5
Leila Riahi, Hanen Chakroun, Imen Klay, Ahmed Slaheddine Masmoudi, Ameur Cherif, Nejia Zoghlami
Background The Mentha rotundifolia L. (Lamiaceae family), is a medicinal herb used since the ancient times as an antiseptic, analgesic and anti-inflammatory agent. In the present work, metabolomic profiling of two Mentha rotundifolia L. ecotypes leaf tissues spontaneously growing in the North of Tunisia was achieved. Methods Phenolic contents (TPC, TFC and TTC) were assessed using colorimetric methods. Metabolomic profiling of leaf tissues extracts was assessed based on Gas Chromatography-Mass Spectrometry (GC/MS) analysis...
October 17, 2018: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/30334458/flavonoid-compounds-and-antibacterial-mechanisms-of-different-parts-of-white-guava-psidium-guajava-l-cv-pearl
#6
Wanjun Zhang, Jingyi Wang, Yashu Chen, Hongbin Zheng, Bijun Xie, Zhida Sun
The flavonoid compositions, extracted from leaves, peel and flesh of white guava (Psidium guajava L. cv. Pearl), were identified and quantified by UPLC-ESI-QTOF-MS, HPLC-ESI-MS/MS and HPLC. The main components of three extracts all were quercetin-glycosides, but the proportion and content of quercetin-hexoside and quercetin-pentoside in each extract were different. Based on the measurements of MIC, MBC value and time killing curve, it emerged that 3 flavonoid extracts of white guava had good antibacterial effects on four pathogenic bacteria...
October 18, 2018: Natural Product Research
https://www.readbyqxmd.com/read/30333809/formation-of-proton-motive-force-under-low-aeration-alkaline-conditions-in-alkaliphilic-bacteria
#7
REVIEW
Toshihide Matsuno, Toshitaka Goto, Shinichi Ogami, Hajime Morimoto, Koji Yamazaki, Norio Inoue, Hidetoshi Matsuyama, Kazuaki Yoshimune, Isao Yumoto
In Mitchell's chemiosmotic theory, a proton (H+ ) motive force across the membrane (Δp), generated by the respiratory chain, drives F1 Fo -ATPase for ATP production in various organisms. The bulk-base chemiosmotic theory cannot account for ATP production in alkaliphilic bacteria. However, alkaliphiles thrive in environments with a H+ concentrations that are one-thousandth (ca. pH 10) the concentration required by neutralophiles. This situation is similar to the production of electricity by hydroelectric turbines under conditions of very limited water...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30332953/synthesis-cytotoxicity-antimicrobial-and-docking-simulation-of-novel-pyrazolo-3-4-d-pyrimidine-and-pyrazolo-4-3-e-1-2-4-triazolo-3-4-c-pyrimidine-derivatives
#8
Hamdi M Hassaneen, Fatma M Saleh, Tayseer A Abdallah, Magda F Mohamed, Yasmin Sh Mohamed, Enas M Awad, Ismail A Abdelhamid
Isobutyrohydrazonoyl bromide 1 was used as precursor for the synthesis of 4-imino-3-isopropyl-1-(4-nitrophenyl)-1,4-dihydro-5H-pyrazolo[3,4-d]pyrimidin-5-amine 4, which was converted to hydrazino derivative 5 by heating with hydrazine hydrate at reflux. Hydrazino as well as imino and amino derivatives undergo condensation and cyclization reactions to give pyrazolo[3,4-d]pyrimidine and pyrazolo[4,3-e][1,2,4]triazolo[3,4-c]pyrimidine derivatives, respectively. Antimicrobial studies are performed using two-gram positive bacteria and two-gram negative bacteria...
October 17, 2018: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/30332951/synthesis-and-docking-study-of-novel-4-thiazolidinone-derivatives-as-anti-gram-positive-and-anti-h-pylori-agents
#9
Armin Khomami, Mohammadamin Rahimi, Arash Tabei, Parastoo Saniee, Arash Mahboubi, Alireza Foroumadi, Nasrin Nassiri Koopaei, Ali Almasirad
BACKGROUND: Bacterial resistance to the available antibiotics is a life threatening issue and researchers are trying to find new drugs to overcome this problem. Amongst the different structural classes, thiazolidinone-4-one as a new effective pharmacophore against various bacteria has been introduced. OBJECTIVE: A new series of 2-(5-(5-nitrothiophene-2-yl)-1,3,4-thiadiazole-2-ylimino)-5-arylidenethiazolidin-4-one derivatives were designed and synthesized as new antibacterial agents...
October 17, 2018: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/30332362/case-259-primary-central-nervous-system-lymphomatoid-granulomatosis-mimicking-chronic-lymphocytic-inflammation-with-pontine-perivascular-enhancement-responsive-to-steroids-clippers
#10
Decai Tian, Xiaodong Zhu, Rong Xue, Peng Zhao, Yuanrong Yao
History In November 2012, a previously healthy 31-year-old woman was admitted to our hospital with a 2-month history of right-sided numbness, diplopia, and intermittent nausea and dizziness. She did not have a history of fever, weight loss, headache, photophobia, seizure, or extremity weakness. Physical examination revealed left abduction limitation and right-sided hypoesthesia. Kernig and Brudzinski signs were absent, and pathergy test results were negative. Laboratory evaluation revealed normal complete and differential blood counts, normal serum chemistry, and normal immune function...
November 2018: Radiology
https://www.readbyqxmd.com/read/30329058/factors-contributing-to-the-variation-in-placental-efficiency-on-days-70-90-and-110-of-gestation-in-gilts
#11
S K Krombeen, W C Bridges, M E Wilson, T A Wilmoth
Variations in placental efficiency (PE), a measure of grams of fetus produced per gram of placenta, were initially researched between swine breeds, where increased PE was associated with larger litters. Placental efficiency was also found to vary greatly within production herds and individual litters; however, the use of PE as a selection tool has been debated. Nonetheless, PE is an index of feto-placental adaptation and may help identify compensatory mechanisms that maintain fetal growth when placental size is reduced, potentially providing an opportunity to address production concerns like low birth weights and preweaning survival...
October 17, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/30328798/structural-dynamic-insights-into-the-h-pylori-cytotoxin-associated-gene-a-caga-and-its-abrogation-to-interact-with-the-tumor-suppressor-protein-aspp2-using-decoy-peptides
#12
Muhammad Junaid, Masaud Shah, Abbas Khan, Cheng-Dong Li, Muhammad Tahir Khan, Aman Chandra Kaushik, Arif Ali, Aamir Mehmood, Asma Sindhoo Nangraj, Sangdun Choi, Dong-Qing Wei
Helicobacter pylori (H. pylori) is one of the most extensively studied gram-negative bacteria due to its implication in gastric cancer. The oncogenicity of H. pylori is associated with cytotoxin-associated gene A (CagA), which is injected into epithelial cells lining the stomach. Both the C- and N-termini of CagA are involved in the interaction with several host proteins, thereby disrupting vital cellular functions, such as cell adhesion, cell cycle, intracellular signal transduction, and cytoskeletal structure...
October 17, 2018: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/30328297/clinical-significance-of-drain-fluid-culture-after-pancreaticoduodenectomy
#13
Feng Yang, Chen Jin, Ji Li, Yang Di, Jing Zhang, Deliang Fu
BACKGROUND: The mechanism of infected postoperative pancreatic fistula (POPF) following PD is undefined. Drain amylase has been used to predict POPF, whereas little data is available about the value of drain fluid culture. The aim was to investigate the incidence, risk factors and association with surgical outcomes of positive drainage culture (PDC) after pancreaticoduodenectomy (PD). METHODS: A single-center retrospective analysis was conducted of prospectively collected data from patients who underwent PD between January 2005 and December 2015...
October 16, 2018: Journal of Hepato-biliary-pancreatic Sciences
https://www.readbyqxmd.com/read/30327644/mechanistic-pathways-for-peptidoglycan-o-acetylation-and-de-o-acetylation
#14
REVIEW
David Sychantha, Ashley S Brott, Carys S Jones, Anthony J Clarke
The post-synthetic O-acetylation of the essential component of bacterial cell walls, peptidoglycan (PG), is performed by many pathogenic bacteria to help them evade the lytic action of innate immunity responses. Occurring at the C-6 hydroxyl of N -acetylmuramoyl residues, this modification to the glycan backbone of PG sterically blocks the activity of lysozymes. As such, the enzyme responsible for this modification in Gram-positive bacteria is recognized as a virulence factor. With Gram-negative bacteria, the O-acetylation of PG provides a means of control of their autolysins at the substrate level...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30326828/global-mrna-decay-and-23s-rrna-fragmentation-in-gluconobacter-oxydans-621h
#15
Angela Kranz, Andrea Steinmann, Ursula Degner, Aliye Mengus-Kaya, Susana Matamouros, Michael Bott, Tino Polen
BACKGROUND: Gluconobacter oxydans is a strictly aerobic Gram-negative acetic acid bacterium used industrially for oxidative biotransformations due to its exceptional type of catabolism. It incompletely oxidizes a wide variety of carbohydrates regio- and stereoselectively in the periplasm using membrane-bound dehydrogenases with accumulation of the products in the medium. As a consequence, only a small fraction of the carbon and energy source enters the cell, resulting in a low biomass yield...
October 16, 2018: BMC Genomics
https://www.readbyqxmd.com/read/30326716/detection-of-virulence-factors-of-south-african-lactococcus-garvieae-isolated-from-rainbow-trout-oncorhynchus-mykiss-walbaum
#16
Cornelia M Meyburgh, Robert R Bragg, Charlotte E Boucher
Lactococcus garvieae is a Gram-positive bacterium that causes mortalities in freshwater and marine fish worldwide and therefore results in severe economic losses in the aquaculture industry. Apart from the apparent integral role of the exopolysaccharide (EPS) capsule in pathogenesis, factors associated with virulence of this bacterium are poorly understood. However, recent studies have indicated that the ability of L. garvieae to cause disease does not depend on the presence of the EPS capsule. Lack of knowledge of virulence factors, pathogenesis and serology of L...
October 4, 2018: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/30326685/treatment-strategy-and-outcomes-in-patients-with-hematogenous-culture-negative-pyogenic-vertebral-osteomyelitis
#17
Gouse Mohamad, Rohit Amritanand, Kenny Samuel David, Venkatesh Krishnan, Justin Arockiaraj
Study Design: Retrospective case series. Purpose: The aim of this study was to analyze functional and radiological outcomes in patients with culture-negative pyogenic vertebral osteomyelitis (PVO). Overview of Literature: There were only few literature available for these group of patients. Methods: Patients with biopsy-positive but culture-negative PVO were included. We analyzed records for data on demography, comorbidities, coexisting infections, neurological status, prior antibiotic therapy, pre- and postoperative erythrocyte sedimentation rate, C-reactive protein levels, and Oswestry Disability Index, and Japanese Orthopedics Association scores...
October 18, 2018: Asian Spine Journal
https://www.readbyqxmd.com/read/30326620/fusion-of-unmanned-aerial-vehicle-panchromatic-and-hyperspectral-images-combining-joint-skewness-kurtosis-figures-and-a-non-subsampled-contourlet-transform
#18
Jinling Zhao, Chengquan Zhou, Linsheng Huang, Xiaodong Yang, Bo Xu, Dong Liang
To obtain fine and potential features, a highly informative fused image created by merging multiple images is usually required. In our study, a novel fusion algorithm called JSKF-NSCT is proposed for fusing panchromatic (PAN) and hyperspectral (HS) images by combining the joint skewness-kurtosis figure (JSKF) and the non-subsampled contourlet transform (NSCT). The JSKF model is used first to derive the three most sensitive bands from the original HS image according to the product of the skewness and the kurtosis coefficients of each band...
October 15, 2018: Sensors
https://www.readbyqxmd.com/read/30326263/new-perspectives-on-mega-plasmid-sequence-poh1-in-bacillus-thuringiensis-atcc-10792-harbouring-antimicrobial-insecticidal-and-antibiotic-resistance-genes
#19
Ramachandran Chelliah, Byung-Jae Park, Shuai Wei, Joong-Hyun Park, Youn-Seo Park, Se-Hun Kim, Yong-Guo Jin, Deog-Hwan Oh
Bacillus thuringiensis promotes the growth of numerous economically important crops. The present study presents the complete genome sequence for a mega plasmid present in the type strain of B. thuringiensis ATCC 10792, a typical spore-forming Gram-positive bacterium with insecticidal activity, and investigates its genetic characteristics. The genome was sequenced and assembled de novo using Pac-Bio sequencers and the Hierarchical Genome Assembly Process, respectively. Further genome annotation was performed, and a total of 489 proteins and a novel mega-plasmid (poh1) with 584,623 bps were identified...
October 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/30325956/peptidoglycan-potentiates-the-membrane-disrupting-effect-of-the-carboxyamidated-form-of-dms-da6-a-gram-positive-selective-antimicrobial-peptide-isolated-from-pachymedusa-dacnicolor-skin
#20
Sébastien Cardon, Emmanuelle Sachon, Ludovic Carlier, Thierry Drujon, Astrid Walrant, Estefanía Alemán-Navarro, Verónica Martínez-Osorio, Dominique Guianvarc'h, Sandrine Sagan, Yannick Fleury, Rodrigue Marquant, Christophe Piesse, Yvonne Rosenstein, Constance Auvynet, Claire Lacombe
The occurrence of nosocomial infections has been on the rise for the past twenty years. Notably, infections caused by the Gram-positive bacteria Staphylococcus aureus represent a major clinical problem, as an increase in antibiotic multi-resistant strains has accompanied this rise. There is thus a crucial need to find and characterize new antibiotics against Gram-positive bacteria, and against antibiotic-resistant strains in general. We identified a new dermaseptin, DMS-DA6, produced by the skin of the Mexican frog Pachymedusa dacnicolor, with specific antibacterial activity against Gram-positive bacteria...
2018: PloS One
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