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Pseudomonas aeruginosa synergies

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https://www.readbyqxmd.com/read/30104278/meropenem-combined-with-ciprofloxacin-combats-hypermutable-pseudomonas-aeruginosa-from-respiratory-infections-of-cystic-fibrosis-patients
#1
Vanessa E Rees, Rajbharan Yadav, Kate E Rogers, Jürgen B Bulitta, Veronika Wirth, Antonio Oliver, John D Boyce, Anton Y Peleg, Roger L Nation, Cornelia B Landersdorfer
Hypermutable Pseudomonas aeruginosa are prevalent in chronic respiratory infections and have been associated with reduced lung function in cystic fibrosis (CF); these isolates can become resistant to all antibiotics in monotherapy. This study aimed to evaluate the time-course of bacterial killing and resistance of meropenem and ciprofloxacin in combination against hypermutable and non-hypermutable P. aeruginosa Static concentration time-kill experiments over 72h assessed meropenem and ciprofloxacin in mono- and combination therapy against PAO1 (non-hypermutable), PAOΔ mutS (hypermutable), and hypermutable isolates CW08, CW35 and CW44 obtained from CF patients with chronic respiratory infections...
August 13, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/30101632/new-anti-pseudomonal-agents-for-cystic-fibrosis-still-needed-in-the-era-of-small-molecule-cftr-modulators
#2
Jane C Davies, Isaac Martin
Cystic fibrosis is characterized by bacterial lung infection, a majority of adults being chronically infected with Pseudomonas aeruginosa. Treatment is a major challenge, with frequent courses of antibiotics contributing to antimicrobial resistance. New approaches are clearly required. Over the last few years, a major shift in our approach to treating CF has occurred with the availability of the first drugs targeting the CFTR protein and leading to improvements in lung function, weight gain and frequency of exacerbations...
August 12, 2018: Expert Opinion on Pharmacotherapy
https://www.readbyqxmd.com/read/30100401/effect-of-sodium-mercaptoacetic-acid-on-different-antimicrobial-disks-in-the-sodium-mercaptoacetic-acid-double-disk-synergy-test-for-detection-of-imp-1-metallo-%C3%AE-lactamase-producing-pseudomonas-aeruginosa-isolates-in-japan
#3
Daisuke Sakanashi, Narimi Miyazaki, Yuzuka Kawamoto, Tomoko Ohno, Atsuko Yamada, Isao Koita, Setsuo Miyajima, Hiroyuki Suematsu, Mao Hagihara, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
We determined the optimal antimicrobial in the sodium mercaptoacetic acid double disk synergy test (SMA-DDST) for the detection of IMP-1-producing Pseudomonas aeruginosa isolates in Japan and evaluated the performance of the test. Fifty-four P. aeruginosa clinical isolates were tested, including 39 IMP-1 producers and 15 non-metallo-β-lactamase (MBL)-producing carbapenem- and ceftazidime (CAZ)-resistant isolates. The SMA-DDST was performed with CAZ, cefepime (CFPM), imipenem (IPM), meropenem (MEPM), doripenem (DRPM), or biapenem (BIPM)-containing disks...
August 9, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/30065794/in-vitro-antibacterial-interaction-of-doripenem-and-amikacin-against-multidrug-resistant-acinetobacter-baumannii-pseudomonas-aeruginosa-and-klebsiella-pneumoniae-isolates
#4
Tonny Loho, Ninik Sukartini, Dalima A W Astrawinata, Suzanna Immanuel, Diana Aulia, Ika Priatni
Evaluation of the in vitro interaction of doripenem and amikacin against Acinetobacter baumannii , Pseudomonas aeruginosa , and Klebsiella pneumoniae was done by classifying them into four groups: doripenem and amikacin sensitive (DOR-S/AMK-S), doripenem sensitive and amikacin resistant (DOR-S/AMK-R), doripenem resistant and amikacin sensitive (DOR-R/AMK-S), and both doripenem and amikacin resistant (DOR-R/AMK-R). The MIC of each antibiotic and their combination was obtained using the Etest method. The fractional inhibitory concentration index was calculated to classify the results as synergistic, additive, indifferent, or antagonistic interaction...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29973719/species-specific-activity-of-antibacterial-drug-combinations
#5
Ana Rita Brochado, Anja Telzerow, Jacob Bobonis, Manuel Banzhaf, André Mateus, Joel Selkrig, Emily Huth, Stefan Bassler, Jordi Zamarreño Beas, Matylda Zietek, Natalie Ng, Sunniva Foerster, Benjamin Ezraty, Béatrice Py, Frédéric Barras, Mikhail M Savitski, Peer Bork, Stephan Göttig, Athanasios Typas
The spread of antimicrobial resistance has become a serious public health concern, making once-treatable diseases deadly again and undermining the achievements of modern medicine1,2 . Drug combinations can help to fight multi-drug-resistant bacterial infections, yet they are largely unexplored and rarely used in clinics. Here we profile almost 3,000 dose-resolved combinations of antibiotics, human-targeted drugs and food additives in six strains from three Gram-negative pathogens-Escherichia coli, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa-to identify general principles for antibacterial drug combinations and understand their potential...
July 2018: Nature
https://www.readbyqxmd.com/read/29928049/synergy-between-conventional-antibiotics-and-anti-biofilm-peptides-in-a-murine-sub-cutaneous-abscess-model-caused-by-recalcitrant-eskape-pathogens
#6
Daniel Pletzer, Sarah C Mansour, Robert E W Hancock
With the antibiotic development pipeline running dry, many fear that we might soon run out of treatment options. High-density infections are particularly difficult to treat due to their adaptive multidrug-resistance and currently there are no therapies that adequately address this important issue. Here, a large-scale in vivo study was performed to enhance the activity of antibiotics to treat high-density infections caused by multidrug-resistant Gram-positive and Gram-negative bacteria. It was shown that synthetic peptides can be used in conjunction with the antibiotics ciprofloxacin, meropenem, erythromycin, gentamicin, and vancomycin to improve the treatment outcome of murine cutaneous abscesses caused by clinical hard-to-treat pathogens including all ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae) pathogens and Escherichia coli...
June 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29875589/metallo-%C3%AE-lactamase-mediated-resistance-among-clinical-carbapenem-resistant-pseudomonas-aeruginosa-isolates-in-northern-iran-a-potential-threat-to-clinical-therapeutics
#7
Abdol Ahad Dogonchi, Ezzat Allah Ghaemi, Abdollah Ardebili, Sajjad Yazdansetad, Abazar Pournajaf
Objective: Carbapenems are effective agents to treat multidrug-resistant (MDR) strains of bacteria, including Pseudomonas aeruginosa . However, there is a potential threat of emergence of carbapenem-resistant P. aeruginosa (CRPA). The aim of this study was to determine antibiotic susceptibility patterns and metallo-beta-lactamase (MBL)-mediated resistance in clinical P. aeruginosa isolates. Materials and Methods: Different clinical specimens were subjected to conventional culture-based identification of P...
April 2018: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
https://www.readbyqxmd.com/read/29772761/high-concentrations-of-sodium-chloride-improve-microbicidal-activity-of-ibuprofen-against-common-cystic-fibrosis-pathogens
#8
Adrián J Muñoz, Roxana V Alasino, Ariel G Garro, Valeria Heredia, Néstor H García, David C Cremonezzi, Dante M Beltramo
Ibuprofen (IBU-H), a widely used anti-inflammatory, also shows a marked antimicrobial effect against several bacterial species, including those involved in cystic fibrosis such as Pseudomona aeruginosa , methicillin resistant Staphylococcus aureus and Burkholderia cepacia complex. Additionally, our results show significant synergy between water soluble Na-ibuprofen (IBU-Na) and ionic strength. Salt concentrations above 0.5 M modify the zeta potential promoting the action of Na-IBU; thus, with 1 M sodium chloride, IBU-Na is ten times more efficient than in the absence of ionic strength, and the minimum effective contact time is reduced from hours to minutes...
May 17, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29756762/molecular-properties-that-define-the-activities-of-antibiotics-in-escherichia-coli-and-pseudomonas-aeruginosa
#9
Sarah J Cooper, Ganesh Krishnamoorthy, David Wolloscheck, John K Walker, Valentin V Rybenkov, Jerry M Parks, Helen I Zgurskaya
The permeability barrier of Gram-negative cell envelopes is the major obstacle in the discovery and development of new antibiotics. In Gram-negative bacteria, these difficulties are exacerbated by the synergistic interaction between two biochemically distinct phenomena, the low permeability of the outer membrane (OM) and active multidrug efflux. In this study, we used Pseudomonas aeruginosa and Escherichia coli strains with controllable permeability barriers, achieved through hyperporination of the OMs and varied efflux capacities, to evaluate the contributions of each of the barriers to protection from antibacterials...
May 25, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29674848/biofilm-infections-between-scylla-and-charybdis-interplay-of-host-antimicrobial-peptides-and-antibiotics
#10
Sergey Chernysh, Natalia Gordya, Dmitry Tulin, Andrey Yakovlev
Purpose: The aim of this study is to improve the anti-biofilm activity of antibiotics. We hypothesized that the antimicrobial peptide (AMP) complex of the host's immune system can be used for this purpose and examined the assumption on model biofilms. Methods: FLIP7, the AMP complex of the blowfly Calliphora vicina containing a combination of defensins, cecropins, diptericins and proline-rich peptides was isolated from the hemolymph of bacteria-challenged maggots...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29581074/antimicrobial-susceptibility-profiles-of-bacteria-causing-urinary-tract-infections-in-mexico-single-centre-experience-with-10-years-of-results
#11
Francisco Alejandro Lagunas-Rangel
OBJECTIVES: The aim of this retrospective study was to describe urinary tract infections (UTIs) diagnosed in a single centre in Mexico through 10 years of results, with a particular emphasis on the aetiology, patterns of antimicrobial susceptibility and incidence of extended-spectrum β-lactamase (ESBL)-producing strains. METHODS: This study involved all urine samples analysed during the period March 2007 to September 2017 in a single centre in Mexico. Isolated uropathogens were identified to species level using routine laboratory methods, and antimicrobial susceptibility was determined by the disk diffusion method...
March 23, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29550930/piperacillin-and-ceftazidime-produce-the-strongest-synergistic-phage-antibiotic-effect-in-pseudomonas-aeruginosa
#12
Jumpei Uchiyama, Ryu Shigehisa, Tadahiro Nasukawa, Keijiro Mizukami, Iyo Takemura-Uchiyama, Takako Ujihara, Hironobu Murakami, Ichiro Imanishi, Koji Nishifuji, Masahiro Sakaguchi, Shigenobu Matsuzaki
The combined use of phage and antibiotics can show synergistic antimicrobial effects, so-called phage-antibiotic synergy (PAS). Here, we screened and examined PAS against Pseudomonas aeruginosa in vitro. Testing four different phages infecting P. aeruginosa, phage KPP22 classified within the family Myoviridae genus Pbunavirus showed PAS with the widest range of antibiotics, and showed PAS with anti-Pseudomonas drugs such as piperacillin and ceftazidime. Thus, evidence suggests that the combined use of phage and antibiotics is a promising therapeutic strategy against P...
July 2018: Archives of Virology
https://www.readbyqxmd.com/read/29483119/efficacy-of-human-simulated-exposures-of-ceftolozane-tazobactam-alone-and-in-combination-with-amikacin-or-colistin-against-multidrug-resistant-pseudomonas-aeruginosa-in-an-in-vitro-pharmacodynamic-model
#13
Veronica Rico Caballero, Safa Almarzoky Abuhussain, Joseph L Kuti, David P Nicolau
Combination therapy is an attractive option for the treatment of multidrug-resistant (MDR) Pseudomonas aeruginosa infections; however, limited data are available on combinations with ceftolozane-tazobactam (C-T). The in vitro pharmacodynamic chemostat model was employed to compare human-simulated exposures of C-T at 3 g every 8 h alone or in combination with amikacin at 25 mg/kg of body weight daily or colistin at 360 mg daily against four MDR P. aeruginosa isolates. C-T alone resulted in 24-h changes in the number of CFU of -0...
May 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29478286/key-role-of-hydrogen-peroxide-in-antimicrobial-activity-of-spring-honeydew-maquis-and-chestnut-grove-corsican-honeys-on-pseudomonas-aeruginosa-dna
#14
J-P Poli, E Guinoiseau, A Luciani, Y Yang, M-J Battesti, J Paolini, J Costa, Y Quilichini, L Berti, V Lorenzi
In honeys, several molecules have been known for their antibacterial or wound healing properties. Corsican honeys just began to be tested for their antimicrobial activity with promising results on Pseudomonas aeruginosa. So, identification of active molecules and their mode of action was determined. Hydrogen peroxide concentrations were evaluated and, in parallel, the minimal inhibitory concentrations (MIC) values were performed with and without catalase. More, the quantity of phenolic compounds and ORAC assay were measured...
May 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29459733/conditional-toxicity-and-synergy-drive-diversity-among-antibacterial-effectors
#15
Kaitlyn D LaCourse, S Brook Peterson, Hemantha D Kulasekara, Matthew C Radey, Jungyun Kim, Joseph D Mougous
Bacteria in polymicrobial habitats contend with a persistent barrage of competitors, often under rapidly changing environmental conditions 1 . The direct antagonism of competitor cells is thus an important bacterial survival strategy 2 . Towards this end, many bacterial species employ an arsenal of antimicrobial effectors with multiple activities; however, the benefits conferred by the simultaneous deployment of diverse toxins are unknown. Here we show that the multiple effectors delivered to competitor bacteria by the type VI secretion system (T6SS) of Pseudomonas aeruginosa display conditional efficacy and act synergistically...
April 2018: Nature Microbiology
https://www.readbyqxmd.com/read/29438821/molecular-characterisation-of-extended-spectrum-%C3%AE-lactamase-producing-gram-negative-bacterial-isolates-from-surgical-wounds-of-patients-at-a-hospital-in-north-central-nigeria
#16
Ahmed Olowo-Okere, Yakubu Kokori Enevene Ibrahim, Busayo Olalekan Olayinka
OBJECTIVES: This study aimed to characterise extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacterial isolates from patients with surgical site infections (SSIs) at a tertiary healthcare facility in Abuja, Nigeria. METHODS: Consecutive, non-duplicate wound swabs were collected over a 3-month period from wounds of patients with SSI and were cultured appropriately. Bacterial isolates were identified using rapid identification kits. The modified agar disk diffusion method was used for antimicrobial susceptibility testing, and phenotypic ESBL activity of the isolates was determined using the double-disk synergy test (DDST)...
February 10, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29432937/detection-of-vim-2-imp-1-and-ndm-1-producing-multidrug-resistant-pseudomonas-aeruginosa-in-malaysia
#17
Siew Mun Liew, Ganeswrei Rajasekaram, Savithri D Puthucheary, Kek Heng Chua
OBJECTIVES: The increasing incidence of carbapenem-resistant Pseudomonas aeruginosa along with the discovery of novel metallo-β-lactamases (MBLs) is of concern. In this study, the isolation of MBL-producing P. aeruginosa clinical strains in Malaysia was investigated. METHODS: A total of 53 P. aeruginosa clinical strains were isolated from different patients in Sultanah Aminah Hospital (Johor Bahru, Malaysia) in 2015. Antimicrobial susceptibility testing was performed, and minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by Etest...
June 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29405135/an-update-on-technical-interpretative-and-clinical-relevance-of-antimicrobial-synergy-testing-methodologies
#18
REVIEW
Shakti Laishram, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Balaji Veeraraghavan
Testing for antimicrobial interactions has gained popularity in the last decade due to the increasing prevalence of drug-resistant organisms and limited options for the treatment of these infections. In vitro combination testing provides information, on which two or more antimicrobials can be combined for a good clinical outcome. Amongst the various in vitro methods of drug interactions, time-kill assay (TKA), checkerboard (CB) assay and E-test-based methods are most commonly used. Comparative performance of these methods reveals the TKA as the most promising method to detect synergistic combinations followed by CB assay and E-test...
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29384621/a-fresh-shine-oncystic-fibrosis-inhalation-therapy-antimicrobial-synergy-of-polymyxin-b-in-combination-with-silver-nanoparticles
#19
Raad Jasim, Elena K Schneider, Meiling Han, Mohammad A K Azad, Maytham Hussein, Cameron Nowell, Mark A Baker, Jiping Wang, Jian Li, Tony Velkov
This in vitro study aimed to investigate the synergistic antibacterial activity of polymyxin B in combination with 2 nm silver nanoparticles (NPs) against Gram-negative pathogens commonly isolated from the cystic fibrosis (CF) lung. The in vitro synergistic activity of polymyxin B with silver NPs was assessed using the checkerboard assay against polymyxinsusceptible and polymyxin-resistant Pseudomonas aeruginosa isolates from the lungs of CF patients. The combination was also examined against the Gram-negative species Haemophilus influenzae, Burkholderia cepacia, Burkholderia pseudomallei, Stenotrophomonas maltophilia, Klebsiella pneumoniae and Acinetobacter baumannii that are less common in the CF lung...
April 2017: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29343818/a-surfactant-polymer-dressing-potentiates-antimicrobial-efficacy-in-biofilm-disruption
#20
Piya Das Ghatak, Shomita S Mathew-Steiner, Priyanka Pandey, Sashwati Roy, Chandan K Sen
A 100% water-soluble surfactant polymer dressing (SPD) that is bio-compatible and non-ionic has been reported to improve wound closure in preliminary clinical studies. The mechanism of action of SPD in wound healing remains unclear. Biofilm infection is a significant problem that hinders proper wound closure. The objective of this study was to characterize the mechanism of action of SPD inhibition of bacterial biofilm development. Static biofilms (48 h) of the primary wound pathogens Pseudomonas aeruginosa (PA01), Staphylococcus aureus (USA300) were grown on polycarbonate membranes and treated with SPD with and without antibiotics for an additional 24 h...
January 17, 2018: Scientific Reports
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