keyword
MENU ▼
Read by QxMD icon Read
search

Pseudomonas aeruginosa resistant synergies

keyword
https://www.readbyqxmd.com/read/30104278/meropenem-combined-with-ciprofloxacin-combats-hypermutable-pseudomonas-aeruginosa-from-respiratory-infections-of-cystic-fibrosis-patients
#1
Vanessa E Rees, Rajbharan Yadav, Kate E Rogers, Jürgen B Bulitta, Veronika Wirth, Antonio Oliver, John D Boyce, Anton Y Peleg, Roger L Nation, Cornelia B Landersdorfer
Hypermutable Pseudomonas aeruginosa are prevalent in chronic respiratory infections and have been associated with reduced lung function in cystic fibrosis (CF); these isolates can become resistant to all antibiotics in monotherapy. This study aimed to evaluate the time-course of bacterial killing and resistance of meropenem and ciprofloxacin in combination against hypermutable and non-hypermutable P. aeruginosa Static concentration time-kill experiments over 72h assessed meropenem and ciprofloxacin in mono- and combination therapy against PAO1 (non-hypermutable), PAOΔ mutS (hypermutable), and hypermutable isolates CW08, CW35 and CW44 obtained from CF patients with chronic respiratory infections...
August 13, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/30101632/new-anti-pseudomonal-agents-for-cystic-fibrosis-still-needed-in-the-era-of-small-molecule-cftr-modulators
#2
Jane C Davies, Isaac Martin
Cystic fibrosis is characterized by bacterial lung infection, a majority of adults being chronically infected with Pseudomonas aeruginosa. Treatment is a major challenge, with frequent courses of antibiotics contributing to antimicrobial resistance. New approaches are clearly required. Over the last few years, a major shift in our approach to treating CF has occurred with the availability of the first drugs targeting the CFTR protein and leading to improvements in lung function, weight gain and frequency of exacerbations...
August 12, 2018: Expert Opinion on Pharmacotherapy
https://www.readbyqxmd.com/read/30100401/effect-of-sodium-mercaptoacetic-acid-on-different-antimicrobial-disks-in-the-sodium-mercaptoacetic-acid-double-disk-synergy-test-for-detection-of-imp-1-metallo-%C3%AE-lactamase-producing-pseudomonas-aeruginosa-isolates-in-japan
#3
Daisuke Sakanashi, Narimi Miyazaki, Yuzuka Kawamoto, Tomoko Ohno, Atsuko Yamada, Isao Koita, Setsuo Miyajima, Hiroyuki Suematsu, Mao Hagihara, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
We determined the optimal antimicrobial in the sodium mercaptoacetic acid double disk synergy test (SMA-DDST) for the detection of IMP-1-producing Pseudomonas aeruginosa isolates in Japan and evaluated the performance of the test. Fifty-four P. aeruginosa clinical isolates were tested, including 39 IMP-1 producers and 15 non-metallo-β-lactamase (MBL)-producing carbapenem- and ceftazidime (CAZ)-resistant isolates. The SMA-DDST was performed with CAZ, cefepime (CFPM), imipenem (IPM), meropenem (MEPM), doripenem (DRPM), or biapenem (BIPM)-containing disks...
August 9, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/30065794/in-vitro-antibacterial-interaction-of-doripenem-and-amikacin-against-multidrug-resistant-acinetobacter-baumannii-pseudomonas-aeruginosa-and-klebsiella-pneumoniae-isolates
#4
Tonny Loho, Ninik Sukartini, Dalima A W Astrawinata, Suzanna Immanuel, Diana Aulia, Ika Priatni
Evaluation of the in vitro interaction of doripenem and amikacin against Acinetobacter baumannii , Pseudomonas aeruginosa , and Klebsiella pneumoniae was done by classifying them into four groups: doripenem and amikacin sensitive (DOR-S/AMK-S), doripenem sensitive and amikacin resistant (DOR-S/AMK-R), doripenem resistant and amikacin sensitive (DOR-R/AMK-S), and both doripenem and amikacin resistant (DOR-R/AMK-R). The MIC of each antibiotic and their combination was obtained using the Etest method. The fractional inhibitory concentration index was calculated to classify the results as synergistic, additive, indifferent, or antagonistic interaction...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29973719/species-specific-activity-of-antibacterial-drug-combinations
#5
Ana Rita Brochado, Anja Telzerow, Jacob Bobonis, Manuel Banzhaf, André Mateus, Joel Selkrig, Emily Huth, Stefan Bassler, Jordi Zamarreño Beas, Matylda Zietek, Natalie Ng, Sunniva Foerster, Benjamin Ezraty, Béatrice Py, Frédéric Barras, Mikhail M Savitski, Peer Bork, Stephan Göttig, Athanasios Typas
The spread of antimicrobial resistance has become a serious public health concern, making once-treatable diseases deadly again and undermining the achievements of modern medicine1,2 . Drug combinations can help to fight multi-drug-resistant bacterial infections, yet they are largely unexplored and rarely used in clinics. Here we profile almost 3,000 dose-resolved combinations of antibiotics, human-targeted drugs and food additives in six strains from three Gram-negative pathogens-Escherichia coli, Salmonella enterica serovar Typhimurium and Pseudomonas aeruginosa-to identify general principles for antibacterial drug combinations and understand their potential...
July 2018: Nature
https://www.readbyqxmd.com/read/29928049/synergy-between-conventional-antibiotics-and-anti-biofilm-peptides-in-a-murine-sub-cutaneous-abscess-model-caused-by-recalcitrant-eskape-pathogens
#6
Daniel Pletzer, Sarah C Mansour, Robert E W Hancock
With the antibiotic development pipeline running dry, many fear that we might soon run out of treatment options. High-density infections are particularly difficult to treat due to their adaptive multidrug-resistance and currently there are no therapies that adequately address this important issue. Here, a large-scale in vivo study was performed to enhance the activity of antibiotics to treat high-density infections caused by multidrug-resistant Gram-positive and Gram-negative bacteria. It was shown that synthetic peptides can be used in conjunction with the antibiotics ciprofloxacin, meropenem, erythromycin, gentamicin, and vancomycin to improve the treatment outcome of murine cutaneous abscesses caused by clinical hard-to-treat pathogens including all ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae) pathogens and Escherichia coli...
June 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29875589/metallo-%C3%AE-lactamase-mediated-resistance-among-clinical-carbapenem-resistant-pseudomonas-aeruginosa-isolates-in-northern-iran-a-potential-threat-to-clinical-therapeutics
#7
Abdol Ahad Dogonchi, Ezzat Allah Ghaemi, Abdollah Ardebili, Sajjad Yazdansetad, Abazar Pournajaf
Objective: Carbapenems are effective agents to treat multidrug-resistant (MDR) strains of bacteria, including Pseudomonas aeruginosa . However, there is a potential threat of emergence of carbapenem-resistant P. aeruginosa (CRPA). The aim of this study was to determine antibiotic susceptibility patterns and metallo-beta-lactamase (MBL)-mediated resistance in clinical P. aeruginosa isolates. Materials and Methods: Different clinical specimens were subjected to conventional culture-based identification of P...
April 2018: Ci Ji Yi Xue za Zhi, Tzu-chi Medical Journal
https://www.readbyqxmd.com/read/29772761/high-concentrations-of-sodium-chloride-improve-microbicidal-activity-of-ibuprofen-against-common-cystic-fibrosis-pathogens
#8
Adrián J Muñoz, Roxana V Alasino, Ariel G Garro, Valeria Heredia, Néstor H García, David C Cremonezzi, Dante M Beltramo
Ibuprofen (IBU-H), a widely used anti-inflammatory, also shows a marked antimicrobial effect against several bacterial species, including those involved in cystic fibrosis such as Pseudomona aeruginosa , methicillin resistant Staphylococcus aureus and Burkholderia cepacia complex. Additionally, our results show significant synergy between water soluble Na-ibuprofen (IBU-Na) and ionic strength. Salt concentrations above 0.5 M modify the zeta potential promoting the action of Na-IBU; thus, with 1 M sodium chloride, IBU-Na is ten times more efficient than in the absence of ionic strength, and the minimum effective contact time is reduced from hours to minutes...
May 17, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29483119/efficacy-of-human-simulated-exposures-of-ceftolozane-tazobactam-alone-and-in-combination-with-amikacin-or-colistin-against-multidrug-resistant-pseudomonas-aeruginosa-in-an-in-vitro-pharmacodynamic-model
#9
Veronica Rico Caballero, Safa Almarzoky Abuhussain, Joseph L Kuti, David P Nicolau
Combination therapy is an attractive option for the treatment of multidrug-resistant (MDR) Pseudomonas aeruginosa infections; however, limited data are available on combinations with ceftolozane-tazobactam (C-T). The in vitro pharmacodynamic chemostat model was employed to compare human-simulated exposures of C-T at 3 g every 8 h alone or in combination with amikacin at 25 mg/kg of body weight daily or colistin at 360 mg daily against four MDR P. aeruginosa isolates. C-T alone resulted in 24-h changes in the number of CFU of -0...
May 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29438821/molecular-characterisation-of-extended-spectrum-%C3%AE-lactamase-producing-gram-negative-bacterial-isolates-from-surgical-wounds-of-patients-at-a-hospital-in-north-central-nigeria
#10
Ahmed Olowo-Okere, Yakubu Kokori Enevene Ibrahim, Busayo Olalekan Olayinka
OBJECTIVES: This study aimed to characterise extended-spectrum β-lactamase (ESBL)-producing Gram-negative bacterial isolates from patients with surgical site infections (SSIs) at a tertiary healthcare facility in Abuja, Nigeria. METHODS: Consecutive, non-duplicate wound swabs were collected over a 3-month period from wounds of patients with SSI and were cultured appropriately. Bacterial isolates were identified using rapid identification kits. The modified agar disk diffusion method was used for antimicrobial susceptibility testing, and phenotypic ESBL activity of the isolates was determined using the double-disk synergy test (DDST)...
February 10, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29432937/detection-of-vim-2-imp-1-and-ndm-1-producing-multidrug-resistant-pseudomonas-aeruginosa-in-malaysia
#11
Siew Mun Liew, Ganeswrei Rajasekaram, Savithri D Puthucheary, Kek Heng Chua
OBJECTIVES: The increasing incidence of carbapenem-resistant Pseudomonas aeruginosa along with the discovery of novel metallo-β-lactamases (MBLs) is of concern. In this study, the isolation of MBL-producing P. aeruginosa clinical strains in Malaysia was investigated. METHODS: A total of 53 P. aeruginosa clinical strains were isolated from different patients in Sultanah Aminah Hospital (Johor Bahru, Malaysia) in 2015. Antimicrobial susceptibility testing was performed, and minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by Etest...
June 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29405135/an-update-on-technical-interpretative-and-clinical-relevance-of-antimicrobial-synergy-testing-methodologies
#12
REVIEW
Shakti Laishram, Agila Kumari Pragasam, Yamuna Devi Bakthavatchalam, Balaji Veeraraghavan
Testing for antimicrobial interactions has gained popularity in the last decade due to the increasing prevalence of drug-resistant organisms and limited options for the treatment of these infections. In vitro combination testing provides information, on which two or more antimicrobials can be combined for a good clinical outcome. Amongst the various in vitro methods of drug interactions, time-kill assay (TKA), checkerboard (CB) assay and E-test-based methods are most commonly used. Comparative performance of these methods reveals the TKA as the most promising method to detect synergistic combinations followed by CB assay and E-test...
October 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29384621/a-fresh-shine-oncystic-fibrosis-inhalation-therapy-antimicrobial-synergy-of-polymyxin-b-in-combination-with-silver-nanoparticles
#13
Raad Jasim, Elena K Schneider, Meiling Han, Mohammad A K Azad, Maytham Hussein, Cameron Nowell, Mark A Baker, Jiping Wang, Jian Li, Tony Velkov
This in vitro study aimed to investigate the synergistic antibacterial activity of polymyxin B in combination with 2 nm silver nanoparticles (NPs) against Gram-negative pathogens commonly isolated from the cystic fibrosis (CF) lung. The in vitro synergistic activity of polymyxin B with silver NPs was assessed using the checkerboard assay against polymyxinsusceptible and polymyxin-resistant Pseudomonas aeruginosa isolates from the lungs of CF patients. The combination was also examined against the Gram-negative species Haemophilus influenzae, Burkholderia cepacia, Burkholderia pseudomallei, Stenotrophomonas maltophilia, Klebsiella pneumoniae and Acinetobacter baumannii that are less common in the CF lung...
April 2017: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29228202/activity-of-ceftazidime-avibactam-against-problem-enterobacteriaceae-and-pseudomonas-aeruginosa-in-the-uk-2015-16
#14
David M Livermore, Danièle Meunier, Katie L Hopkins, Michel Doumith, Robert Hill, Rachel Pike, Peter Staves, Neil Woodford
Background: Ceftazidime/avibactam combines an established oxyimino-cephalosporin with the first diazabicyclooctane β-lactamase inhibitor to enter clinical use. We reviewed its activity against Gram-negative isolates, predominantly from the UK, referred for resistance investigation in the first year of routine testing, beginning in July 2015. Methods: Isolates were as received from referring laboratories; there is a bias to submit those with suspected carbapenem resistance...
December 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29205266/amphiphilic-lysine-conjugated-to-tobramycin-synergizes-legacy-antibiotics-against-wild-type-and-multidrug-resistant-pseudomonas-aeruginosa
#15
Yinfeng Lyu, Ronald Domalaon, Xuan Yang, Frank Schweizer
Peptidomimetic modification is a common route of optimization for biologically active peptides. Previous studies in our group have shown that conjugation of amphiphilic tobramycin to other antibacterials enhance their latent outer membrane permeabilizing and efflux blocking activity toward Gram-negative pathogens including Pseudomonas aeruginosa. Herein, we describe the antimicrobial adjuvant properties of amphiphilic lysine ligated to tobramycin. The most potent amphiphilic lysine-tobramycin conjugate 3 potentiated the antibacterial efficacy of 8 clinically used antibiotics against wild type, multidrug- and extensively drug-resistant P...
December 4, 2017: Biopolymers
https://www.readbyqxmd.com/read/29094001/in-vitro-synergy-of-antibiotic-combinations-against-planktonic-and-biofilm-pseudomonas-aeruginosa
#16
Hossein Ghorbani, Mohammad Yousef Memar, Fatemeh Yeganeh Sefidan, Mina Yekani, Reza Ghotaslou
Aim: The combination of different antimicrobial agents and subsequent synergetic effects may be beneficial in treatment of P. aeruginosa infections. The aim of the present study was to determine antibiotic susceptibility patterns of clinical isolates of P. aeruginosa and the effect of different antibiotic combinations against the multidrug-resistant (MDR), biofilm-producing bacterium P. aeruginosa . Methods: Thirty-six P. aeruginosa clinical isolates were evaluated. The disk diffusion method was performed to determine antibiotic susceptibility patterns according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
2017: GMS Hygiene and Infection Control
https://www.readbyqxmd.com/read/29093703/caprylic-and-polygalacturonic-acid-combinations-for-eradication-of-microbial-organisms-embedded-in-biofilm
#17
Joel Rosenblatt, Ruth A Reitzel, Nylev Vargas-Cruz, Anne-Marie Chaftari, Ray Hachem, Issam Raad
There is a need for non-antibiotic, antimicrobial compositions with low toxicity capable of broad-spectrum eradication of pathogenic biofilms in food preparation and healthcare settings. In this study we demonstrated complete biofilm eradication within 60 min with synergistic combinations of caprylic and polygalacturonic (PG) acids in an in vitro biofilm eradication model against representative hospital and foodborne infectious pathogen biofilms (methicillin-resistant Staphylococcus aureus , multidrug-resistant Pseudomonas aeruginosa , Candida albicans , Escherichia coli , and Salmonella enteritidis )...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29089426/synergy-between-active-efflux-and-outer-membrane-diffusion-defines-rules-of-antibiotic-permeation-into-gram-negative-bacteria
#18
Ganesh Krishnamoorthy, Inga V Leus, Jon W Weeks, David Wolloscheck, Valentin V Rybenkov, Helen I Zgurskaya
Gram-negative bacteria are notoriously resistant to antibiotics, but the extent of the resistance varies broadly between species. We report that in significant human pathogens Acinetobacter baumannii , Pseudomonas aeruginosa , and Burkholderia spp., the differences in antibiotic resistance are largely defined by their penetration into the cell. For all tested antibiotics, the intracellular penetration was determined by a synergistic relationship between active efflux and the permeability barrier. We found that the outer membrane (OM) and efflux pumps select compounds on the basis of distinct properties and together universally protect bacteria from structurally diverse antibiotics...
October 31, 2017: MBio
https://www.readbyqxmd.com/read/29058043/synergistic-antimicrobial-activity-between-the-broad-spectrum-bacteriocin-garvicin-ks-and-nisin-farnesol-and-polymyxin-b-against-gram-positive-and-gram-negative-bacteria
#19
Hai Chi, Helge Holo
The increasing emergence of antibiotics resistance is of global concern. Finding novel antimicrobial agents and strategies based on synergistic combinations are essential to combat resistant bacteria. We evaluated the activity of garvicin KS, a new bacteriocin produced by Lactococcus garvieae. The bacteriocin has a broad inhibitory spectrum, inhibiting members of all the 19 species of Gram-positive bacteria tested. Unlike other bacteriocins from Gram-positive bacteria, garvicin KS inhibits Acinetobacter but not other Gram-negative bacteria...
March 2018: Current Microbiology
https://www.readbyqxmd.com/read/28966485/evaluation-of-different-phenotypic-tests-for-detection-of-metallo-%C3%AE-lactamases-in-imipenem-resistant-pseudomonas-aeruginosa
#20
Rohit Sachdeva, Babita Sharma, Rajni Sharma
PURPOSE: Pseudomonas aeruginosa causes a wide spectrum of infections including bacteremia, pneumonia, urinary tract infection, etc., Metallo-beta-lactamase (MBL) producing P. aeruginosa is an emerging threat and cause of concern as they have emerged as one of the most feared resistance mechanisms. This study was designed to know the prevalence of MBL production in P. aeruginosa and to evaluate the four phenotypic tests for detection of MBL production in imipenem-resistant clinical isolates of P...
October 2017: Journal of Laboratory Physicians
keyword
keyword
164912
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"