Read by QxMD icon Read


Jae Min Lee, Sun Sil Choi, Yo Han Lee, Keon Woo Khim, Sora Yoon, Byung-Gyu Kim, Dougu Nam, Pann-Ghill Suh, Kyungjae Myung, Jang Hyun Choi
Peroxisome proliferator-activated receptor gamma (PPARγ) is a ligand-dependent transcription factor that regulates adipocyte differentiation and glucose homeostasis. The transcriptional activity of PPARγ is regulated not only by ligands but also by post-translational modifications (PTMs). In this study, we demonstrate that a novel E3 ligase of PPARγ, tripartite motif-containing 25 (TRIM25), directly induced the ubiquitination of PPARγ, leading to its proteasome-dependent degradation. During adipocyte differentiation, both TRIM25 mRNA and protein expression significantly decreased and negatively correlated with the expression of PPARγ...
October 15, 2018: Experimental & Molecular Medicine
Hye-Lim Lee, Abdul S Qadir, Hyun-Jung Park, Eunkyung Chung, Yun-Sil Lee, Kyung Mi Woo, Hyun-Mo Ryoo, Hyun Jeong Kim, Jeong-Hwa Baek
Distal-less homeobox 5 (Dlx5) is a negative regulator of adipogenesis. Dlx5 expression is decreased by adipogenic stimuli, but the mechanisms of Dlx5 downregulation by adipogenic stimuli have not yet been determined. Here, we tested the impact of cAMP/PKA (protein kinase A) signaling induced by 3-isobutyl-1 methyl xanthine (IBMX), forskolin, and 8-CPT-cAMP on the expression of Dlx5 in 3T3-L1 preadipocytes. Significant downregulation of Dlx5 mRNA expression and protein production levels were observed via cAMP/PKA-dependent signaling...
October 14, 2018: International Journal of Molecular Sciences
Yu-Lin Pan, Rui Xin, Sheng-Yuan Wang, Yue Wang, Lin Zhang, Cui-Ping Yu, Yong-Hui Wu
Nickel (Ni) is widely present in the occupational environment and causes various adverse effects on the human body. Apoptosis induced by Ni2+ may be a key mechanism underlying its toxic effect. In the present study, we investigated the effect of Ni-smelting fumes on cell viability, mitochondrial damage, and apoptosis-related proteins in NIH/3T3 cells. The effects of Ni-smelting fumes at concentrations of 0, 25, 50, and 100 μg/mL were tested. Treatment with Ni-smelting fumes for 24 h and 48 h significantly decreased cell viability and lactate dehydrogenase activity in a dose- and time-dependent manner compared with the blank control group...
October 12, 2018: Archives of Biochemistry and Biophysics
Subramaniam Sri Devi, Natarajan Ashokkumar
Obesity is considered as a major global human health problem which significantly increases the risk for development of type 2 diabetes. Citral, a bioactive compound widely found in a variety of foods that are consumed daily. In this study, we investigated the inhibitory effect of citral against adipogenic genes in 3T3-L1 cells. The mouse fibroblast 3T3-L1 pre-adipocytes were differentiated into adipocytes using adipogenic cocktail (5 g/ml insulin, 0.5 mM isobutylmethylxanthine and 10 M dexamethasone). Differentiation of adipocytes was evaluated by assessing triglyceride accumulation assay and cell viability by MTT assay...
October 2018: Indian Journal of Clinical Biochemistry: IJCB
Alessio Massironi, Andrea Morelli, Lucia Grassi, Dario Puppi, Simona Braccini, Giuseppantonio Maisetta, Semih Esin, Giovanna Batoni, Cristina Della Pina, Federica Chiellini
Silver nanoparticles (AgNPs) have been intensively investigated in virtue of their optical and antimicrobial properties, although their applications have been limited due to inherent toxicity and to the need of employing harsh chemical reagents for the synthesis. In this work, ulvan, a sulfated polysaccharide extracted from green algae belonging to Ulva armoricana sp., was for the first time investigated and identified as reducing and stabilizing agent for AgNPs synthesis by using milder conditions than those conventionally adopted by chemical methods...
January 1, 2019: Carbohydrate Polymers
Nahla Issa, Gabriel Lachance, Kerstin Bellmann, Mathieu Laplante, Krisztian Stadler, Andre Marette
NADPH oxidase enzymes (NOX) are one of the major superoxide-generating systems in cells. NOX-generated superoxide has been suggested to promote insulin resistance in liver. However, the role of NOX enzymes in mediating metabolic dysfunction in other insulin target tissues remains unclear. Here we show that NADPH oxidase 3 (NOX3) expression is induced in differentiated 3T3-L1 adipocytes upon treatment with pro-inflammatory cytokines. Superoxide production increased concurrently with NOX3 protein expression in cytokine-treated adipocytes, which was inhibited by the NOX inhibitor diphenyleneiodonium (DPI)...
October 12, 2018: Journal of Lipid Research
Jeferson Gustavo Henn, Luiza Steffens, Nathalia Denise de Moura Sperotto, Betânia de Souza Ponce, Rodrigo Moisés Veríssimo, Fernanda Brião Menezes Boaretto, Gustavo Hassemer, Valéria Flores Péres, Helena Schirmer, Jaqueline Nascimento Picada, Jenifer Saffi, Dinara Jaqueline Moura
ETHNOPHARMACOLOGICAL RELEVANCE: Plantago australis is a perennial plant widely distributed in Latin America, and its seeds and leaves are used in folk medicine to treat many diseases and conditions. Among its various chemical compounds, verbascoside is one of the most present, and has several pharmacological activities described, but there is not much information about its toxicity. AIMS OF THE STUDY: The aims of this study were to optimize the extraction of verbascoside from P...
October 11, 2018: Journal of Ethnopharmacology
Bullo Saifullah, Kalaivani Buskaran, Rabia Baby Shaikh, Farahnaz Barahuie, Sharida Fakurazi, Mohd Aris Mohd Moklas, Mohd Zobir Hussein
The treatment of cancer through chemotherapy is limited by its toxicity to healthy tissues and organs, and its inability to target the cancer site. In this study, we have designed an anticancer nanocomposite delivery system for protocatechuic acid (PCA) using graphene oxide⁻polyethylene glycol as the nanocarrier, and coated with folic acid (GO⁻PEG⁻PCA⁻FA) for targeting the cancer cells. The designed anticancer delivery system was found to show much better anticancer activity than the free drug PCA against liver cancer HEP-G2 cells and human colon cancer HT-29 cells; at same time, it was found to be less toxic to normal fibroblast 3T3 cells...
October 11, 2018: Nanomaterials
Lijie Wei, Yuan Chen, Wenqiang Tan, Qing Li, Guodong Gu, Fang Dong, Zhanyong Guo
In this study, a series of triple quaternized chitosan derivatives, including 6- O -[(2-hydroxy-3-trimethylammonium)propyl]-2- N -(1-pyridylmethyl-2-ylmethyl)- N , N -dimethyl chitosan chloride ( 7 ), 6- O -[(2-hydroxy-3-trimethylammonium)propyl]-2- N -(1-pyridylmethyl-3-yl- methyl)- N , N -dimethyl chitosan chloride ( 8 ), and 6- O -[(2-hydroxy-3-trimethylammonium)propyl]- 2- N -(1-pyridylmethyl-4-ylmethyl)- N , N -dimethyl chitosan chloride ( 9 ) were successfully designed and synthesized via reacting epoxypropyl trimethylammonium chloride with the N -pyridinium double quaternized chitosan derivatives...
October 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Krishnamoorthy Vijayalakshmi, Chinnadurai Immanuel Selvaraj
Nature has gifted us with abundant plants possessing medicinal virtues which can cure several illnesses. Currently, diabetes mellitus is a severe threat to human well-being across the world due to the rapidly increasing incidence of diabetes. New effective bioactive drugs are in need, as plants do harbour and are proven to have potential antidiabetic activity than the present hypoglycemic medicines used in clinical therapy. In this study, in vitro cytotoxicity, glucose uptake, and anti-adipogenic activities of the plant extract (methanolic extract) of S...
October 11, 2018: Molecular Biology Reports
Jyoti Shrestha Takanche, Ji-Eun Kim, Jeong-Seok Kim, Min-Ho Lee, Jae-Gyu Jeon, Il-Song Park, Ho-Keun Yi
Osseointegration of dental implants is affected by osteoporosis. The purpose of this study was overcome the implant failure and facilitate the osseointegration of dental implants by c-myb in ovariectomized (OVX)-induced osteoporosis. c-myb is a transcription factor and supports bone formation. Plasmid DNA/c-myb conjugated with chitosan-gold nanoparticles (Ch-GNPs/c-myb) promoted osteogenesis and inhibited osteoclastogenesis in MC-3T3 E1 cells. Ch-GNPs/c-myb involved the reduction of the nuclear factor of activated T-cells 1, c-Fos, and tartrate-resistant acid phosphatase-positive multinucleated osteoclasts in receptor activator of nuclear factor-κB ligand (RANKL) stimulated bone marrow macrophages...
October 11, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Mengistu Lemecha, Katsutaro Morino, Takeshi Imamura, Hirotaka Iwasaki, Natsuko Ohashi, Shogo Ida, Daisuke Sato, Osamu Sekine, Satoshi Ugi, Hiroshi Maegawa
Mitochondria are critical in heat generation in brown and beige adipocytes. Mitochondrial number and function are regulated in response to external stimuli, such as cold exposure and β3 adrenergic receptor agonist. However, the molecular mechanisms regulating mitochondrial biogenesis during browning, especially by microRNAs, remain unknown. We investigated the role of miR-494-3p in mitochondrial biogenesis during adipogenesis and browning. Intermittent mild cold exposure of mice induced PPARγ coactivator1-α (PGC1-α) and mitochondrial TFAM, PDH, and ANT1/2 expression along with uncoupling protein-1 (Ucp1) in inguinal white adipose tissue (iWAT)...
October 10, 2018: Scientific Reports
Pawel A Kolodziejski, Ewa Pruszynska-Oszmalek, Maciej Micker, Marek Skrzypski, Tatiana Wojciechowicz, Patryk Szwarckopf, Kinga Skieresz-Szewczyk, Krzysztof W Nowak, Mathias Z Strowski
Spexin (SPX, NPQ) is a novel peptide involved in the regulation of energy metabolism. SPX inhibits food intake and reduces body weight. In obese humans, SPX is the most down-regulated gene in fat. Therefore, SPX might be involved in the regulation of lipid metabolism. Here, we study the effects of SPX on lipolysis, lipogenesis, glucose uptake, adipogenesis, cell proliferation and survival in isolated human adipocytes or murine 3T3-L1 cells. SPX and its receptors, GALR2 and GALR3, are present at mRNA and protein levels in murine 3T3-L1 cells and human adipocytes...
October 2018: Biochimica et biophysica acta. Molecular and cell biology of lipids
Masayoshi Ishida, Naoyuki Kawao, Kiyotaka Okada, Kohei Tatsumi, Kazuko Sakai, Kazuto Nishio, Hiroshi Kaji
It is well known that sex differences exist about the severity of osteoporosis and bone metabolism, suggesting that factors other than sex hormones might be responsible for sex differences of bone metabolism. We therefore examined sex differences of the osteoblast phenotypes of mouse osteoblasts, and then performed the comparative gene expression analyses using a comprehensive DNA microarray between female and male osteoblasts. Alkaline phosphatase (ALP) activity, mineralization and the expression of Osterix, ALP, and bone sialoprotein were significantly lower in mouse female osteoblasts, compared to male...
October 9, 2018: Endocrinology
J Almeida, J Costa, P Coelho, V Cea, M Galesio, J P Noronha, M S Diniz, C Prudêncio, R Soares, C Sala, Rúben Fernandes
Gliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis...
October 9, 2018: Metabolic Brain Disease
Tiago R Silva, Ramon Canela-Garayoa, Jordi Eras, Marili V N Rodrigues, Fábio N Dos Santos, Marcos N Eberlin, Iramaia A Neri-Numa, Glaucia M Pastore, Renata S N Tavares, Hosana M Debonsi, Lorena R G Cordeiro, Luiz H Rosa, Valéria M Oliveira
An iridescent yellow pigmented bacterium isolated from the Antarctic continent, named Cellulophaga fucicola strain 416, was found to be able to tolerate UV-B radiation. Its crude pigment extract was tested for antioxidant capacity, UV light stability and phototoxicity profile against murine fibroblast lines. The pigments were further isolated and chemically identified by ultra-high-performance liquid chromatography with photodiode array and mass spectrometry detectors. The results showed that the pigment extract presented weak stability under exposure to UV light, a phototoxic profile in the 3t3 Neutral Red Uptake test and a very high antioxidant activity, suggesting that it could be used as food and feed colourants...
October 9, 2018: Antonie Van Leeuwenhoek
Jiazhen Tang, Hongyi Zhou, Khushboo Sahay, Wenqiong Xu, Jing Yang, Wei Zhang, Weiqin Chen
BACKGROUND: Obesity and its associated morbidities represent the major and most rapidly expanding world-wide health epidemic. Recent genome-wide association studies (GWAS) reveal that single nucleotide polymorphism (SNP) variant in the Family with Sequence Similarity 13, Member A (FAM13A) gene is strongly associated with waist-hip ratio (WHR) with adjustment for body mass index (BMI) (WHRadjBMI). However, the function of FAM13A in adipose development and obesity remains largely uncharacterized...
October 9, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Aifaa Akmal Baharuddin, Rushduddin Al Jufri Roosli, Zainul Amiruddin Zakaria, Siti Farah Md Tohid
CONTEXT: Dicranopteris linearis (Burm.f.) Underw. (Gleicheniaceae) has been scientifically proven to exert various pharmacological activities. Nevertheless, its anti-proliferative potential has not been extensively investigated. OBJECTIVE: To investigate the anti-proliferative potential of D. linearis leaves and determine possible mechanistic pathways. MATERIALS AND METHODS: MTT assay was used to determine the cytotoxic effects of D. linearis methanol (MEDL) and petroleum ether (PEEDL) extracts at concentrations of 100, 50, 25, 12...
December 2018: Pharmaceutical Biology
Ji-Ping Li, Ye Yuan, Wen-You Zhang, Zhen Jiang, Tian-Jiao Hu, Yi-Ting Feng, Mo-Xiang Liu
OBJECTIVES: The objective of this paper was to explore the effects of Radix isatidis polysaccharide (RIP) extracted from Radix isatis on alleviating insulin resistance. METHODS: The insulin resistance models of 3T3-L1 preadipocytes and type 2 diabetic rats were established to evaluate the insulin resistance activity of RIP. KEY FINDINGS: Radix isatidis polysaccharide within the concentration range of 25-100 μg/ml could reduce cell supernatant glucose and TNF-α levels (P < 0...
October 9, 2018: Journal of Pharmacy and Pharmacology
Jing Ma, Hanfang Liu, Zhuoqing Xi, Jiuzhou Hou, Yingguang Li, Jie Niu, Tong Liu, Shuning Bi, Xin Wang, Chaojie Wang, Jiajia Wang, Songqiang Xie, Peng G Wang
Physiological characteristics of human malignancies are increased glycolysis and overexpression of glucose transporters (GLUTs). 18 Flurodeoxyglucose-positron emission tomography (FDG-PET) has successfully developed as clinical modality for the diagnosis and staging of many cancers based on the Warburg effect. To leverage this glucose transporter mediated metabolic disparity between normal and malignant cells, in the current report, protected, and de-protected glucose, mannose, galactose, rhamnose, maltose, and lactose-conjugated platinum(IV) complexes were designed and synthesized...
2018: Frontiers in Chemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"