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https://www.readbyqxmd.com/read/30238981/laminar-shear-stress-provoked-cytoskeletal-changes-are-mediated-by-epigenetic-reprogramming-of-timp1-in-human-primary-smooth-muscle-cells
#1
Rodrigo A da Silva, Célio Jr da C Fernandes, Geórgia da S Feltran, Anderson M Gomes, Amanda Fantini de Camargo Andrade, Denise C Andia, Maikel P Peppelenbosch, Willian F Zambuzzi
Whereas endothelial responses to shear stress are well-characterized, the cell physiological effects of shear stress in smooth muscle cells (SMCs) remain largely obscure. As SMCs are directly challenged by shear stress after endothelial denuding injury following procedures such as angioplasty or endarterectomy, characterization of these responses represents an important scientific question. Hence we decided to contrast cytoskeletal reorganization, epigenetic reprogramming, signaling transduction, and changes in miRNA (miRs) profiles in primary human aortic smooth muscle cells (AoSMCs) between unstressed cells and cells exposed to shear stress...
September 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28710961/epigenetic-modification-differences-between-fetal-fibroblast-cells-and-mesenchymal-stem-cells-of-the-arbas-cashmere-goat
#2
Xiao Wang, Zhimin Wang, Qing Wang, Hefei Wang, Hao Liang, Dongjun Liu
To explore the epigenetic mechanisms regulating mesenchymal stem cells, we analyzed epigenetic patterns in control goat fetal fibroblast cells (gFFCs), adipose-derived stem cells (gADSCs), bone marrow stromal cells (gBMSCs), and muscle-derived satellite cells (gMDSCs). We found that the 5mC content of gBMSC genomes was lower than that of gFFC genomes, while the 5mC content of gADSC and gMDSC genomes surpassed that of gFFC genomes. H3K9 acetylation did not differ significantly among those cells; gFFCs, gADSCs, and gMDSCs contained acetylated H3K9, H3K14, H3K18, H4K5, and H4K12, but gBMSCs contained almost no acetylated H4K5 and H4K12...
October 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28272491/ten-eleven-translocation-2-tet2-is-involved-in-myogenic-differentiation-of-skeletal-myoblast-cells-in-vitro
#3
Xia Zhong, Qian-Qian Wang, Jian-Wei Li, Yu-Mei Zhang, Xiao-Rong An, Jian Hou
Muscle cell differentiation is a complex process that is principally governed by related myogenic regulatory factors (MRFs). DNA methylation is considered to play an important role on the expression of MRF genes and on muscle cell differentiation. However, the roles of enzymes specifically in myogenesis are not fully understood. Here, we demonstrate that Tet2, a ten-eleven translocation (Tet) methylcytosine dioxygenase, exerts a role during skeletal myoblast differentiation. By using an immunostaining method, we found that the levels of 5-hydroxymethylcytosine (5-hmC) were much higher in differentiated myotubes than in undifferentiated C2C12 myoblasts...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27349543/myod-is-regulated-by-the-mir-29a-tet1-pathway-in-c2c12-myoblast-cells
#4
Akiyoshi Chikenji, Hitoshi Ando, Megumi Nariyama, Takeo Suga, Ryohei Iida, Kazuhiro Gomi
Skeletal myogenesis is regulated by a considerable number of microRNAs (miRNAs). miRNA regulatory networks are complicated, and details of how they operate remain unclear. In this study, MTT assays confirmed that miR-29a is the most effective miR-29 paralog. Microarray analysis demonstrated upregulation of ten-eleven translocation enzyme-1 (Tet1) mRNA in response to miR-29a inhibition in C2C12 murine myoblast cells. We investigated the factors acting downstream in the miR-29a-Tet1 signal pathway using real-time RT-PCR...
2016: Journal of Oral Science
https://www.readbyqxmd.com/read/26472283/inactivation-of-sdh-and-fh-cause-loss-of-5hmc-and-increased-h3k9me3-in-paraganglioma-pheochromocytoma-and-smooth-muscle-tumors
#5
Attje S Hoekstra, Marieke A de Graaff, Inge H Briaire-de Bruijn, Cor Ras, Reza Maleki Seifar, Ivonne van Minderhout, Cees J Cornelisse, Pancras C W Hogendoorn, Martijn H Breuning, Johnny Suijker, Esther Korpershoek, Henricus P M Kunst, Norma Frizzell, Peter Devilee, Jean-Pierre Bayley, Judith V M G Bovée
Succinate dehydrogenase (SDH) and fumarate hydratase (FH) are tricarboxylic acid (TCA) cycle enzymes and tumor suppressors. Loss-of-function mutations give rise to hereditary paragangliomas/pheochromocytomas and hereditary leiomyomatosis and renal cell carcinoma. Inactivation of SDH and FH results in an abnormal accumulation of their substrates succinate and fumarate, leading to inhibition of numerous α-ketoglutarate dependent dioxygenases, including histone demethylases and the ten-eleven-translocation (TET) family of 5-methylcytosine (5 mC) hydroxylases...
November 17, 2015: Oncotarget
https://www.readbyqxmd.com/read/25993995/alternation-of-histone-and-dna-methylation-in-human-atherosclerotic-carotid-plaques
#6
COMPARATIVE STUDY
A Greißel, M Culmes, R Napieralski, E Wagner, H Gebhard, M Schmitt, A Zimmermann, H-H Eckstein, A Zernecke, J Pelisek
Little is known about epigenetics and its possible role in atherosclerosis. We here analysed histone and DNA methylation and the expression of corresponding methyltransferases in early and advanced human atherosclerotic carotid lesions in comparison to healthy carotid arteries. Western Blotting was performed on carotid plaques from our biobank with early (n=60) or advanced (n=60) stages of atherosclerosis and healthy carotid arteries (n=12) to analyse di-methylation patterns of histone H3 at positions K4, K9 and K27...
August 2015: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/25549186/rational-design-and-engineering-of-a-modified-adeno-associated-virus-aav1-based-vector-system-for-enhanced-retrograde-gene-delivery
#7
Adam S Davis, Thais Federici, William C Ray, Nicholas M Boulis, Deirdre OʼConnor, K Reed Clark, Jeffrey S Bartlett
BACKGROUND: After injection into muscle and peripheral nerves, a variety of viral vectors undergo retrograde transport to lower motor neurons. However, because of its attractive safety profile and durable gene expression, adeno-associated virus (AAV) remains the only vector to have been applied to the human nervous system for the treatment of neurodegenerative disease. Nonetheless, only a very small fraction of intramuscularly injected AAV vector arrives at the spinal cord. OBJECTIVE: To engineer a novel AAV vector by inserting a neuronal targeting peptide (Tet1), with binding properties similar to those of tetanus toxin, into the AAV1 capsid...
February 2015: Neurosurgery
https://www.readbyqxmd.com/read/23417056/early-de-novo-dna-methylation-and-prolonged-demethylation-in-the-muscle-lineage
#8
Koji Tsumagari, Carl Baribault, Jolyon Terragni, Katherine E Varley, Jason Gertz, Sirharsa Pradhan, Melody Badoo, Charlene M Crain, Lingyun Song, Gregory E Crawford, Richard M Myers, Michelle Lacey, Melanie Ehrlich
Myogenic cell cultures derived from muscle biopsies are excellent models for human cell differentiation. We report the first comprehensive analysis of myogenesis-specific DNA hyper- and hypo-methylation throughout the genome for human muscle progenitor cells (both myoblasts and myotubes) and skeletal muscle tissue vs. 30 non-muscle samples using reduced representation bisulfite sequencing. We also focused on four genes with extensive hyper- or hypo-methylation in the muscle lineage (PAX3, TBX1, MYH7B/MIR499 and OBSCN) to compare DNA methylation, DNaseI hypersensitivity, histone modification, and CTCF binding profiles...
March 2013: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/16023583/a-novel-peptide-defined-through-phage-display-for-therapeutic-protein-and-vector-neuronal-targeting
#9
COMPARATIVE STUDY
James K Liu, Qingshan Teng, Mary Garrity-Moses, Thais Federici, Diana Tanase, Michael J Imperiale, Nicholas M Boulis
A novel peptide with the binding characteristics of tetanus toxin was identified with phage display, for application in therapeutic protein and vector motor and sensory neuron targeting. A 12mer phage library was biopanned on trisialoganglioside (G(T1b)) and eluted with the tetanus toxin C fragment (rTTC). Phage ELISAs revealed increases in G(T1b) binding for the Tet1 and Tet2 phage clones when compared to peptideless phage (PLP). rTTC displaced both Tet1 and Tet2 phage clones from G(T1b), and both clones reduced rTTC-G(T1b) binding...
August 2005: Neurobiology of Disease
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