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ankyrin-R

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https://www.readbyqxmd.com/read/27247322/severe-ankyrin-r-deficiency-results-in-impaired-surface-retention-and-lysosomal-degradation-of-rhag-in-human-erythroblasts
#1
Timothy J Satchwell, Amanda J Bell, Bethan R Hawley, Stephanie Pellegrin, Kathryn E Mordue, Cees Th B M van Deursen, Nicole Heitink-Ter Braak, Gerwin Huls, Mathie P G Leers, Eline Overwater, Rienk Y J Tamminga, Bert van der Zwaag, Elisa Fermo, Paola Bianchi, Richard van Wijk, Ashley M Toye
Ankyrin-R provides a key link between band 3 and the spectrin cytoskeleton that helps to maintain the highly specialized erythrocyte biconcave shape. Ankyrin deficiency results in fragile spherocytic erythrocytes with reduced band 3 and protein 4.2 expression. We use in vitro differentiation of erythroblasts transduced with shRNAs targeting ANK1 to generate erythroblasts and reticulocytes with a novel ankyrin-R 'near null' human phenotype with less than 5% of normal ankyrin expression. Using this model, we demonstrate that absence of ankyrin negatively impacts the reticulocyte expression of a variety of proteins, including band 3, glycophorin A, spectrin, adducin and, more strikingly, protein 4...
September 2016: Haematologica
https://www.readbyqxmd.com/read/26821241/cerebellar-ataxias-%C3%AE-iii-spectrin-s-interactions-suggest-common-pathogenic-pathways
#2
REVIEW
Emma Perkins, Daumante Suminaite, Mandy Jackson
Spinocerebellar ataxias (SCAs) are a genetically heterogeneous group of disorders all characterised by postural abnormalities, motor deficits and cerebellar degeneration. Animal and in vitro models have revealed β-III spectrin, a cytoskeletal protein present throughout the soma and dendritic tree of cerebellar Purkinje cells, to be required for the maintenance of dendritic architecture and for the trafficking and/or stabilisation of several membrane proteins: ankyrin-R, cell adhesion molecules, metabotropic glutamate receptor-1 (mGluR1), voltage-gated sodium channels (Nav ) and glutamate transporters...
August 15, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/25616663/evidence-of-a-structural-and-functional-ammonium-transporter-rhbg%C3%A2-anion-exchanger-1%C3%A2-ankyrin-g-complex-in-kidney-epithelial-cells
#3
Sandrine Genetet, Pierre Ripoche, Caroline Le Van Kim, Yves Colin, Claude Lopez
The renal ammonium transporter RhBG and anion exchanger 1 kAE1 colocalize in the basolateral domain of α-intercalated cells in the distal nephron. Although we have previously shown that RhBG is linked to the spectrin-based skeleton through ankyrin-G and that its NH3 transport activity is dependent on this association, there is no evidence for an interaction of kAE1 with this adaptor protein. We report here that the kAE1 cytoplasmic N terminus actually binds to ankyrin-G, both in yeast two-hybrid analysis and by coimmunoprecipitation in situ in HEK293 cells expressing recombinant kAE1...
March 13, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25362473/a-hierarchy-of-ankyrin-spectrin-complexes-clusters-sodium-channels-at-nodes-of-ranvier
#4
Tammy Szu-Yu Ho, Daniel R Zollinger, Kae-Jiun Chang, Mingxuan Xu, Edward C Cooper, Michael C Stankewich, Vann Bennett, Matthew N Rasband
The scaffolding protein ankyrin-G is required for Na(+) channel clustering at axon initial segments. It is also considered essential for Na(+) channel clustering at nodes of Ranvier to facilitate fast and efficient action potential propagation. However, notwithstanding these widely accepted roles, we show here that ankyrin-G is dispensable for nodal Na(+) channel clustering in vivo. Unexpectedly, in the absence of ankyrin-G, erythrocyte ankyrin (ankyrin-R) and its binding partner βI spectrin substitute for and rescue nodal Na(+) channel clustering...
December 2014: Nature Neuroscience
https://www.readbyqxmd.com/read/24603075/%C3%AE-iii-spectrin-underpins-ankyrin-r-function-in-purkinje-cell-dendritic-trees-protein-complex-critical-for-sodium-channel-activity-is-impaired-by-sca5-associated-mutations
#5
Yvonne L Clarkson, Emma M Perkins, Callum J Cairncross, Alastair R Lyndon, Paul A Skehel, Mandy Jackson
Beta III spectrin is present throughout the elaborate dendritic tree of cerebellar Purkinje cells and is required for normal neuronal morphology and cell survival. Spinocerebellar ataxia type 5 (SCA5) and spectrin associated autosomal recessive cerebellar ataxia type 1 are human neurodegenerative diseases involving progressive gait ataxia and cerebellar atrophy. Both disorders appear to result from loss of β-III spectrin function. Further elucidation of β-III spectrin function is therefore needed to understand disease mechanisms and identify potential therapeutic options...
July 15, 2014: Human Molecular Genetics
https://www.readbyqxmd.com/read/24090929/interaction-of-plasmodium-falciparum-knob-associated-histidine-rich-protein-kahrp-with-erythrocyte-ankyrin-r-is-required-for-its-attachment-to-the-erythrocyte-membrane
#6
Haibo Weng, Xinhua Guo, Julien Papoin, Jie Wang, Ross Coppel, Narla Mohandas, Xiuli An
The malaria parasite Plasmodium falciparum exports a large number of proteins into the erythrocyte cytoplasm during the asexual intraerythrocytic stage of its life cycle. A subset of these proteins interacts with erythrocyte membrane skeletal proteins and grossly alters the structure and function of the membrane. Several of the exported proteins, such as PfEMP1, PfEMP3, RESA and KAHRP, interact with the preponderant erythrocyte skeleton protein, spectrin. Here we have searched for possible interaction of these four malaria proteins with another major erythrocyte skeleton protein, ankyrin R...
January 2014: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/23051696/atomic-force-microscopy-captures-folded-ribosome-bound-nascent-chains
#7
Anna Loksztejn, Zackary Scholl, Piotr E Marszalek
Direct visualization of co-translational folding of nascent polypeptide chains is challenging. Here we present, for the first time, AFM images of large protein constructs based on the membrane binding domain of ankyrin-R, complexed with the ribosome. The characteristic "horse-shoe" shape of ankyrin-R emerging from the ribosome was captured.
December 14, 2012: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/22778271/ankyrin-b-protein-in-heart-failure-identification-of-a-new-component-of-metazoan-cardioprotection
#8
Farshid Kashef, Jingdong Li, Patrick Wright, Jedidiah Snyder, Faroug Suliman, Ahmet Kilic, Robert S D Higgins, Mark E Anderson, Philip F Binkley, Thomas J Hund, Peter J Mohler
Ankyrins (ankyrin-R, -B, and -G) are adapter proteins linked with defects in metazoan physiology. Ankyrin-B (encoded by ANK2) loss-of-function mutations are directly associated with human cardiovascular phenotypes including sinus node disease, atrial fibrillation, ventricular tachycardia, and sudden cardiac death. Despite the link between ankyrin-B dysfunction and monogenic disease, there are no data linking ankyrin-B regulation with common forms of human heart failure. Here, we report that ankyrin-B levels are altered in both ischemic and non-ischemic human heart failure...
August 31, 2012: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/22420867/weak-d-caused-by-a-founder-deletion-in-the-rhd-gene
#9
Yann Fichou, Jian-Min Chen, Cédric Le Maréchal, Déborah Jamet, Isabelle Dupont, Claude Chuteau, Cécile Durousseau, Marie-Jeanne Loirat, Pascal Bailly, Claude Férec
BACKGROUND: The RhD blood group system exemplifies a genotype-phenotype correlation by virtue of its highly polymorphic and immunogenic nature. Weak D phenotypes are generally thought to result from missense mutations leading to quantitative change of the D antigen in the red blood cell membrane or intracellularly. STUDY DESIGN AND METHODS: Different sets of polymerase chain reaction primers were designed to map and clone a deletion involving RHD Exon 10, which was found in approximately 3% of approximately 2000 RHD hemizygous subjects with D phenotype ambiguity...
November 2012: Transfusion
https://www.readbyqxmd.com/read/22411828/structure-of-the-zu5-zu5-upa-dd-tandem-of-ankyrin-b-reveals-interaction-surfaces-necessary-for-ankyrin-function
#10
Chao Wang, Cong Yu, Fei Ye, Zhiyi Wei, Mingjie Zhang
Ankyrin-R/B/G (encoded by ANK1/2/3, respectively) are a family of very large scaffold proteins capable of anchoring numerous receptors and ion channels to specific, spectrin-containing membrane micro-domains. Hereditary mutations of ankyrins are known to be associated with diseases including spherocytosis, cardiac arrhythmia, and bipolar disorder in humans, although the underlying molecular bases are poorly understood. The middle spectrin-binding domain of ankyrins contains highly conserved ZU5-ZU5-UPA-DD domains arranged into the ZZUD tandem...
March 27, 2012: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/22404934/mechanical-anisotropy-of-ankyrin-repeats
#11
Whasil Lee, Xiancheng Zeng, Kristina Rotolo, Ming Yang, Christopher J Schofield, Vann Bennett, Weitao Yang, Piotr E Marszalek
Red blood cells are frequently deformed and their cytoskeletal proteins such as spectrin and ankyrin-R are repeatedly subjected to mechanical forces. While the mechanics of spectrin was thoroughly investigated in vitro and in vivo, little is known about the mechanical behavior of ankyrin-R. In this study, we combine coarse-grained steered molecular dynamics simulations and atomic force spectroscopy to examine the mechanical response of ankyrin repeats (ARs) in a model synthetic AR protein NI6C, and in the D34 fragment of native ankyrin-R when these proteins are subjected to various stretching geometry conditions...
March 7, 2012: Biophysical Journal
https://www.readbyqxmd.com/read/21542582/phosphorylation-dependent-perturbations-of-the-4-1r-associated-multiprotein-complex-of-the-erythrocyte-membrane
#12
Emilie Gauthier, Xinhua Guo, Narla Mohandas, Xiuli An
The bulk of the red blood cell membrane proteins are partitioned between two multiprotein complexes, one associated with ankyrin R and the other with protein 4.1R. Here we examine the effect of phosphorylation of 4.1R on its interactions with its partners in the membrane. We show that activation of protein kinase C in the intact cell leads to phosphorylation of 4.1R at two sites, serine 312 and serine 331. This renders the 4.1R-associated transmembrane proteins GPC, Duffy, XK, and Kell readily extractable by nonionic detergent with no effect on the retention of band 3 and Rh, both of which also interact with 4...
May 31, 2011: Biochemistry
https://www.readbyqxmd.com/read/21502821/conserved-residues-in-the-ankyrin-domain-of-vapyrin-indicate-potential-protein-protein-interaction-surfaces
#13
Nadja Feddermann, Didier Reinhardt
Plant VAPYRINS are required for the establishment of arbuscular mycorrhiza (AM) and root nodule symbiosis (RNS). In vapyrin mutants, the intracellular accommodation of AM fungi and rhizobia is blocked, and in the case of AM, the fungal endosymbiont cannot develop arbuscules which serve for nutrient exchange. VAPYRINs are plant-specific proteins that consists of a major sperm protein (MSP) domain and an ankyrin domain. Comparison of VAPYRINS of dicots, monocots, and the moss Physcomitrella patens reveals a highly conserved domain structure...
May 2011: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/21493712/determination-of-structural-models-of-the-complex-between-the-cytoplasmic-domain-of-erythrocyte-band-3-and-ankyrin-r-repeats-13-24
#14
Sunghoon Kim, Suzanne Brandon, Zheng Zhou, Charles E Cobb, Sarah J Edwards, Christopher W Moth, Christian S Parry, Jarrod A Smith, Terry P Lybrand, Eric J Hustedt, Albert H Beth
The adaptor protein ankyrin-R interacts via its membrane binding domain with the cytoplasmic domain of the anion exchange protein (AE1) and via its spectrin binding domain with the spectrin-based membrane skeleton in human erythrocytes. This set of interactions provides a bridge between the lipid bilayer and the membrane skeleton, thereby stabilizing the membrane. Crystal structures for the dimeric cytoplasmic domain of AE1 (cdb3) and for a 12-ankyrin repeat segment (repeats 13-24) from the membrane binding domain of ankyrin-R (AnkD34) have been reported...
June 10, 2011: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/20550922/fast-and-forceful-refolding-of-stretched-alpha-helical-solenoid-proteins
#15
Minkyu Kim, Khadar Abdi, Gwangrog Lee, Mahir Rabbi, Whasil Lee, Ming Yang, Christopher J Schofield, Vann Bennett, Piotr E Marszalek
Anfinsen's thermodynamic hypothesis implies that proteins can encode for stretching through reversible loss of structure. However, large in vitro extensions of proteins that occur through a progressive unfolding of their domains typically dissipate a significant amount of energy, and therefore are not thermodynamically reversible. Some coiled-coil proteins have been found to stretch nearly reversibly, although their extension is typically limited to 2.5 times their folded length. Here, we report investigations on the mechanical properties of individual molecules of ankyrin-R, beta-catenin, and clathrin, which are representative examples of over 800 predicted human proteins composed of tightly packed alpha-helical repeats (termed ANK, ARM, or HEAT repeats, respectively) that form spiral-shaped protein domains...
June 16, 2010: Biophysical Journal
https://www.readbyqxmd.com/read/20101027/structural-basis-for-spectrin-recognition-by-ankyrin
#16
Jonathan J Ipsaro, Alfonso Mondragón
Maintenance of membrane integrity and organization in the metazoan cell is accomplished through intracellular tethering of membrane proteins to an extensive, flexible protein network. Spectrin, the principal component of this network, is anchored to membrane proteins through the adaptor protein ankyrin. To elucidate the atomic basis for this interaction, we determined a crystal structure of human betaI-spectrin repeats 13 to 15 in complex with the ZU5-ANK domain of human ankyrin R. The structure reveals the role of repeats 14 to 15 in binding, the electrostatic and hydrophobic contributions along the interface, and the necessity for a particular orientation of the spectrin repeats...
May 20, 2010: Blood
https://www.readbyqxmd.com/read/19299464/a-golgi-associated-protein-4-1b-variant-is-required-for-assimilation-of-proteins-in-the-membrane
#17
Qiaozhen Kang, Ting Wang, Huizheng Zhang, Narla Mohandas, Xiuli An
The archetypal membrane skeleton is that of the erythrocyte, consisting predominantly of spectrin, actin, ankyrin R and protein 4.1R. The presence in the Golgi of a membrane skeleton with a similar structure has been inferred, based on the identification of Golgi-associated spectrin and ankyrin. It has long been assumed that a Golgi-specific protein 4.1 must also exist, but it has not previously been found. We demonstrate here that a hitherto unknown form of protein 4.1, a 200 kDa 4.1B, is associated with the Golgi of Madin-Darby canine kidney (MDCK) and human bronchial epithelial (HBE) cells...
April 15, 2009: Journal of Cell Science
https://www.readbyqxmd.com/read/18768923/ankyrin-facilitates-intracellular-trafficking-of-alpha1-na-k-atpase-in-polarized-cells
#18
Paul R Stabach, Prasad Devarajan, Michael C Stankewich, Serguei Bannykh, Jon S Morrow
Defects in ankyrin underlie many hereditary disorders involving the mislocalization of membrane proteins. Such phenotypes are usually attributed to ankyrin's role in stabilizing a plasma membrane scaffold, but this assumption may not be accurate. We found in Madin-Darby canine kidney cells and in other cultured cells that the 25-residue ankyrin-binding sequence of alpha(1)-Na(+)-K(+)-ATPase facilitates the entry of alpha(1),beta(1)-Na(+)-K(+)-ATPase into the secretory pathway and that replacement of the cytoplasmic domain of vesicular stomatitis virus G protein (VSV-G) with this ankyrin-binding sequence bestows ankyrin dependency on the endoplasmic reticulum (ER) to Golgi trafficking of VSV-G...
November 2008: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/16580865/functional-interaction-between-rh-proteins-and-the-spectrin-based-skeleton-in-erythroid-and-epithelial-cells
#19
REVIEW
V Nicolas, I Mouro-Chanteloup, C Lopez, P Gane, A Gimm, N Mohandas, J-P Cartron, C Le Van Kim, Y Colin
We summarize the different experimental approaches which provide evidence that direct interaction of Rh and RhAG to ankyrin-R constitutes, together with the AE-1 (Band 3)-ankyrin-protein 4.2 and GPC-protein 4.1-p55 complexes, another major anchoring site between the red cell membrane bilayer and the underlying spectrin-based skeleton. The observations that some residues of the ankyrin binding site are mutated in Rh and RhAG proteins from some weak D and Rh(null) variants, respectively, suggest that the Rh-RhAG/ankyrin-R interaction plays a crucial role in the biosynthesis and/or the stability of the Rh complex in the red cell membrane...
March 2006: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/16415852/nanospring-behaviour-of-ankyrin-repeats
#20
Gwangrog Lee, Khadar Abdi, Yong Jiang, Peter Michaely, Vann Bennett, Piotr E Marszalek
Ankyrin repeats are an amino-acid motif believed to function in protein recognition; they are present in tandem copies in diverse proteins in nearly all phyla. Ankyrin repeats contain antiparallel alpha-helices that can stack to form a superhelical spiral. Visual inspection of the extrapolated structure of 24 ankyrin-R repeats indicates the possibility of spring-like behaviour of the putative superhelix. Moreover, stacks of 17-29 ankyrin repeats in the cytoplasmic domains of transient receptor potential (TRP) channels have been identified as candidates for a spring that gates mechanoreceptors in hair cells as well as in Drosophila bristles...
March 9, 2006: Nature
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