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Fresh transfer

Samer Tannus, Yoni Cohen, Sara Henderson, Na'ama Al Ma'mari, Tal Shavit, Weon-Young Son, Michael H Dahan
STUDY QUESTION: In IVF cycles in which the entire embryo cohort is slow growing, is it optimal to perform fresh transfer in Day 5 or to extend the culture and transfer in subsequent vitrified-warmed cycles? SUMMARY ANSWER: The outcomes depend on the degree of embryo development on Day 5. WHAT IS KNOWN ALREADY: Slow-growing blastocysts have lower implantation potential when transferred in fresh cycles. It has been suggested that embryo-endometrial asynchrony could explain this finding...
December 4, 2018: Human Reproduction
Bin Wang, Cheng Tang, Hao-Fan Wang, Xiao Chen, Rui Cao, Qiang Zhang
A cost-effective and highly efficient oxygen evolution reaction (OER) electrocatalyst will be significant for the future energy scenario. The emergence of the core-shell heterostructure has invoked new feasibilities to inspire the full potential of non-precious-metal candidates. The shells always have a large thickness, affording robust mechanical properties under harsh reaction conditions, which limits the full exposure of active sites with highly intrinsic reactivity and extrinsic physicochemical characters for optimal performance...
December 5, 2018: Advanced Materials
Mohamad Assad, Michel Chalot, Fabienne Tatin-Froux, Valérie Bert, Julien Parelle
The development of a biomonitor in the context of multiple-element contamination in urban environments was tested by comparing element transfer in edible crops and poplar ( Torr. × A. Henry cutlivar 'Skado'). A multielemental analysis was performed with various common edible crops (cucumber [ L.], pepper [ L.], cabbage [ L.], and lettuce [ L.]) and the Skado poplar cultivar grown on soils that received sediments dredged from water canals in the 1960s. Sediments were distributed unevenly on the soil, allowing us to sample two types of areas that were either weakly (Area 1) or highly (Area 2) contaminated, mainly by Cd, Pb, and Zn...
November 2018: Journal of Environmental Quality
Andrea Osimani, Vesna Milanović, Federica Cardinali, Cristiana Garofalo, Francesca Clementi, Sara Ruschioni, Paola Riolo, Nunzio Isidoro, Nino Loreto, Roberta Galarini, Simone Moretti, Annalisa Petruzzelli, Eleonora Micci, Franco Tonucci, Lucia Aquilanti
In the present study, the distribution of antibiotic resistance genes in laboratory-reared fresh mealworm larvae ( Tenebrio molitor L.), their feeding substrates (carrots and wheatmeal), and frass was assessed. Microbial counts on selective media added with antibiotics highlighted the presence of lactic acid bacteria resistant to ampicillin and vancomycin and, more specifically, enterococci resistant to the latter antibiotic. Moreover, staphylococci resistant to gentamicin, erythromycin, tetracycline, and vancomycin were detected...
2018: Frontiers in Microbiology
Madhubari Vathulya, Mohd Salahuddin Ansari
Introduction: The superficial veins of upper limbs are seldom talked about. They are always avoided quoting them to be variable in anatomy. Other than the cephalic and basilic veins, no other superficial vein has caught the eyes of anatomists and other health professionals significantly. This article tries to explain the importance of a superficial vein that closely traces the radial vessels and explains its possible use in microvascular reconstruction. Materials and Methods: The forelimbs of 26 upper limbs of fresh frozen cadavers were dissected out...
May 2018: Indian Journal of Plastic Surgery: Official Publication of the Association of Plastic Surgeons of India
Uma Mariappen, Kevin N Keane, Peter M Hinchliffe, Satvinder S Dhaliwal, John L Yovich
Advanced age is an increasing trend for both males and females seeking in vitro fertilization (IVF). This retrospective cohort study investigated the outcomes of 1280 IVF-related treatment cycles, selecting the first treatment for couples utilizing autologous gametes and who underwent single fresh embryo transfer. Males aged 40-49 years had a 52% reduction in normal sperm motility, while it was markedly reduced by 79% at 50 years or older. However, neither semen parameters nor male age were predictive of clinical pregnancy or live birth chance...
November 29, 2018: Reproductive Biology
Rachel B Mejia, Taylor W Cox, Edward B Nguyen, Karen M Summers, Patrick Ten Eyck, Amy E Sparks, Bradley J Van Voorhis
OBJECTIVE: To evaluate which clinical characteristics influence early maternal β-human chorionic gonadotropin (hCG) and progesterone levels in in vitro fertilization (IVF) pregnancies. DESIGN: Retrospective cohort analysis. SETTING: Academic medical center. PATIENT(S): Women with a live birth after single-blastocyst embryo transfer in either a fresh or frozen cycle between 2004 and 2017, comprising 1,282 pregnancies in 1,057 patients...
December 2018: Fertility and Sterility
Bo Yu, Rani Fritz, Xianhong Xie, Abdissa Negassa, Sangita Jindal, Mario Vega, Erkan Buyuk
OBJECTIVE: To assess the impact of using donor sperm in assisted reproductive technology (ART) cycles on perinatal outcomes. DESIGN: Historical cohort study. SETTING: US national database from the Society of Assisted Reproductive Technology Clinic Outcome Reporting System (SART CORS) from 2012 to 2013. PATIENT(S): Patients undergoing the first fresh autologous ART cycle using either donor or partner sperm. INTERVENTION(S): None...
December 2018: Fertility and Sterility
Mahmoud Moussa, Chun-Yan Yang, Hai-Ying Zheng, Meng-Qi Li, Nong-Qi Yu, Sheng-Fei Yan, Jia-Xiang Huang, Jiang-Hua Shang
The objectives of the present study were to investigate the effect of vitrification on the expression of the key genes associated with blastocyst developmental potential (β-catenin, E-cadherin, Oct-4, Cdx2, Gata3), and whether the presence of β-mercaptoethanol (β-ME, 100 μM) in in vitro culture (IVC) media will affect the expression of these genes. Buffalo pre-implantation embryos were divided into three groups: (1) fresh non-vitrified embryos were used as control, (2) vitrified embryos cultured with β-ME (+), and (3) vitrified embryos cultured without (-) β-ME...
November 20, 2018: Theriogenology
Lauren A Murphy, Emily A Seidler, Denis A Vaughan, Nina Resetkova, Alan S Penzias, Thomas L Toth, Kim L Thornton, Denny Sakkas
STUDY QUESTION: What is the treatment path and cumulative live birth (CLB) rate from a single oocyte retrieval of patients who intend to pursue PGT-A at the start of an IVF cycle compared to matched controls? SUMMARY ANSWER: The choice of PGT-A at the start of the first IVF cycle decreases the CLB per oocyte retrieval for patients <38 years of age, however patients ≥38 years of age benefit significantly per embryo transfer (ET) when live birth (LB) is evaluated...
November 30, 2018: Human Reproduction
Sandiswa Mbewana, Ann E Meyers, Edward P Rybicki
Rift valley fever virus (RVFV) is an emerging mosquito borne virus and haemorrhagic fever agent, which causes abortion storms in farmed small ruminants and potentially causes miscarriages in humans. Although live-attenuated vaccines are available for animals, they can only be used in endemic areas and there are currently no commercially available vaccines for humans. Here we describe the production of chimaeric RVFV virus-like particles transiently expressed in Nicotiana benthamiana by Agrobacterium tumefaciens-mediated gene transfer...
November 28, 2018: Biotechnology Journal
Panagiotis Drakopoulos, Joaquín Errázuriz, Samuel Santos-Ribeiro, Herman Tournaye, Alberto Vaiarelli, Nicola Pluchino, Christophe Blockeel, Nikolaos Polyzos
INTRODUCTION: Traditionally, in vitro fertilization (IVF) success rates have been reported in terms of live birth per fresh cycle or embryo transfer. However, reporting IVF outcomes using cumulative live birth rates (LBR), defined as the first live birth following the use of all fresh and frozen embryos derived from a single ovarian stimulation cycle appears to be a better measure of IVF treatment success. EVIDENCE ACQUISITION AND SYNTHESIS: Although there is an initial increase in fresh LBR with the number of oocytes retrieved, LBR either reach a plateau or may even decline when more than 15-20 oocytes are harvested...
November 27, 2018: Minerva Ginecologica
Depeng Meng, Huihao Chen, Yaofa Lin, Haodong Lin, Chunlin Hou
BACKGROUND: Sciatic nerve injuries cause significant disability. We propose here a novel reconstructive procedure of transferring the motor branches of the femoral nerve as donor nerves to reconstruct both the peroneal and tibial nerve function as a novel approach to treat high sciatic nerve injury. METHODS: The autopsies of donor nerves (vastus lateralis nerve branch (VLN), vastus medialis nerve branch (VMN), saphenous nerve (SAN)) and respective recipient nerves (deep peroneal nerve branch (DPN), medial gastrocnemius nerve branch (MGN), sural nerve (SN)) were conducted in six fresh-frozen lower limbs...
November 27, 2018: Acta Neurochirurgica
Ajanto Kumar Hazarika, Somdeb Chanda, Santanu Sabhapondit, Sandip Sanyal, Pradip Tamuly, Sahnaz Tasrin, Dilip Sing, Bipan Tudu, Rajib Bandyopadhyay
This paper reports on the development of an integrated leaf quality inspecting system using near infrared reflectance (NIR) spectroscopy for quick and in situ estimation of total polyphenol (TP) content of fresh tea leaves, which is the most important quality indicator of tea. The integrated system consists of a heating system to dry the fresh tea leaves to the level of 3-4% moisture, a grinding and sieving system fitted with a 250 micron mesh sieve to make fine powder from the dried leaf. Samples thus prepared are transferred to the NIR beam and TP is measured instantaneously...
December 2018: Journal of Food Science and Technology
Mark Hann, Stephen A Roberts, Stephen W D'Souza, Peter Clayton, Nick Macklon, Daniel R Brison
BACKGROUND: Birth weight and early child growth are important predictors of long-term cardiometabolic disease risk, in line with the Developmental Origins of Health and Disease hypothesis. As human assisted reproductive technologies (ARTs) occur during the sensitive periconceptional window of development, it has recently become a matter of urgency to investigate risk in ART-conceived children. METHODS: We have conducted the first large-scale, national cohort study of early growth in ART children from birth to school age, linking the register of ART, held by the UK's Human Fertilisation and Embryology Authority, to Scottish maternity and child health databases...
November 28, 2018: BMC Medicine
Ernesto Bosch, Carlo Bulletti, Alan B Copperman, Renato Fanchin, Hakan Yarali, Carlos A Petta, Nikolaos P Polyzos, Daniel Shapiro, Filippo Maria Ubaldi, Juan A Garcia Velasco, Salvatore Longobardi, Thomas D'Hooghe, Peter Humaidan
RESEARCH QUESTION: How might time to healthy singleton delivery affect decision-making during infertility treatment? DESIGN: This was a Delphi consensus investigating expert opinion that comprised three steps. In Step 1, 12 experts developed statements. In Step 2, 27 experts (including 12 from Step 1) voted (online survey) on their agreement/disagreement with each statement (providing reasons). Consensus was reached if ≥66% of participants agreed/disagreed. Statements not reaching consensus were revised and the process repeated until consensus was achieved...
November 7, 2018: Reproductive Biomedicine Online
Maria-Theresia Gekenidis, Weihong Qi, Jörg Hummerjohann, Reinhard Zbinden, Fiona Walsh, David Drissner
Irrigation water is a major source of fresh produce contamination with undesired microorganisms including antibiotic-resistant bacteria (ARB), and contaminated fresh produce can transfer ARB to the consumer especially when consumed raw. Nevertheless, no legal guidelines exist so far regulating quality of irrigation water with respect to ARB. We therefore examined irrigation water from major vegetable growing areas for occurrence of antibiotic-resistant indicator bacteria Escherichia coli and Enterococcus spp...
2018: PloS One
Tácia Gomes Bergstein-Galan, Romildo Romualdo Weiss, Luiz Ernandes Kozicki
Multiple ovulation and embryo transfer (MOET) is an important tool in the sheep industry for increasing numbers of genetically superior individuals. The objective of this study was to evaluate the effect of semen source (frozen or fresh), the number of embryo collection procedures for each donor (NECP), the season in which embryo transfer and collection was performed, and the age and breed of the donor, on the number of recovered embryos and pregnancy rates after embryo transfer. The Alamos Genetics' flushing station database was used...
November 25, 2018: Reproduction in Domestic Animals, Zuchthygiene
Nasibeh Hajilary, Mashallah Rezakazemi
The gas sweetening unit of phase 2 and 3 in South Pars Gas Field (Asalouyeh, Iran) was first simulated to investigate the effect of mono ethylene glycol (MEG) in the amine loop. MEG is commonly injected into the system to avoid hydrate formation while a few amounts of MEG is usually transferred to amine gas sweetening plant. This paper aims to address the points where MEG has negative effects on gas sweetening process and what the practical ways to reduce its effect are. The results showed that in the presence of 25% of MEG in amine loop, H2 S absorption from the sour gas was increased from 1...
November 23, 2018: Chemistry Central Journal
Geng Leng, Qiong Hu, Wen-Feng He, Zhe Liu, Wen-Jin Chen, Wen-Bo Xu, Qiu-Hong Yang, Jing Sun
Sulfite is known to be harmful to human health and associated to sulfur related environmental effects and ideally should be analyzed onsite owing to its instability. Here we describe an automated, miniaturized, and highly efficient dispersive liquid-liquid microextraction (DLLME) system that seamlessly coupled to a UV-vis spectrophotometer for the trace analysis of sulfite in natural waters. The automated DLLME system was constructed by a single syringe pump that is coupled with a multiposition valve. Nanomolar levels of sulfite could be extracted from natural water samples and injected into the hyphenated spectrophotometer for quantification...
November 13, 2018: Journal of Chromatography. A
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