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Fresh transfer

Loic Delens, Grégory Ehx, Joan Somja, Louise Vrancken, Ludovic Belle, Laurence Seidel, Céline Grégoire, Gilles Fransolet, Caroline Ritacco, Muriel Hannon, Sophie Dubois, Yves Beguin, Frédéric Baron, Sophie Servais
Acute graft-versus-host disease (aGVHD) is a severe complication of allogeneic hematopoietic stem cell transplantation. The role of Th17 cells in its pathophysiology remains debated. In this study, we assessed whether enrichment of human peripheral blood mononuclear cells (PBMCs) with in vitro Th17-polarized CD4+ T cells would exacerbate xenogeneic GVHD (xGVHD) into NOD-scid IL-2Rγnull (NSG) mice. Naive human CD4+ T cells were stimulated under Th17-skewing conditions for 8-10 days before being co-injected in NSG mice with fresh PBMCs from the same donor...
October 13, 2018: Biology of Blood and Marrow Transplantation
Anna M Modest, Lauren A Wise, Matthew P Fox, Jennifer Weuve, Alan S Penzias, Michele R Hacker
STUDY QUESTION: Does inverse probability weighting (IPW) provide a more valid estimate of the cumulative incidence of live birth after multiple cycles of IVF? SUMMARY ANSWER: IPW can provide a more accurate estimate of treatment success for counseling and decision-making regarding IVF. WHAT IS KNOWN ALREADY: Different approaches have been used to define and calculate IVF success; however, many of these approaches have limitations and potentially violate statistical assumptions...
October 15, 2018: Human Reproduction
Lise Haaber Thomsen, Ulrik Schiøler Kesmodel, Claus Yding Andersen, Peter Humaidan
No abstract text is available yet for this article.
October 15, 2018: Human Reproduction
C A Venetis, B W Mol, E M Kolibianakis
No abstract text is available yet for this article.
October 15, 2018: Human Reproduction
Hiroki Umezawa, Takeshi Matsutani, Kazuhiko Yokoshima, Munenaga Nakamizo, Rei Ogawa
Background: Patients with head and neck or esophageal cancer who undergo resection and reconstructive surgery sometimes develop fistulae that exhibit delayed wound healing. We developed a novel negative pressure wound therapy (NPWT) that employs a Penrose drain. This case series report describes its effect on the wound healing and treatment duration of cancer patients with postoperative fistulae. Methods: This consecutive case series consisted of all patients from February 2014 to February 2017 who underwent resection and reconstruction for head and neck or esophageal cancer and who then developed a fistula that was treated with either NPWT or a second flap that did not resolve the fistula or led to fistula recurrence and was then treated with NPWT...
August 2018: Plastic and Reconstructive Surgery. Global Open
Xia Xiao, Xiaoyuan He, Qing Li, Huan Zhang, Juanxia Meng, Yanyu Jiang, Qi Deng, Mingfeng Zhao
BACKGROUND: Tumor immunotherapy with chimeric antigen receptor T cells (CAR-T) is a promising new treatment for B-cell malignancies and has produced exciting results. However, cytokine release syndrome (CRS) is the most significant toxicity associated with this treatment and can be life-threatening. CASE PRESENTATION: A 23-year-old male patient had been diagnosed with relapsed and refractory B-cell acute lymphocytic leukemia. The patient was recruited into our CAR-T clinical trial, and 1×106 /kg of engineered anti-CD19 CAR-T cells was administered...
October 15, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Celine Blank, Imke T Duijf, Els Slappendel, Massimo Mischi, Saskia Houterman, Jacques W M Maas, Petra de Sutter, Benedictus C Schoot
OBJECTIVE: To evaluate the multivariate embryo selection model by van Loendersloot et al. (2014) (VL) in a different geographical context. DESIGN: This is a retrospective external validation study of a 5-year cohort of women undergoing in vitro fertilization or intracytoplasmatic sperm injection. SETTING: Two outpatient fertility clinics. PATIENT(S): A total of 1,197 women who underwent 1,610 fresh in vitro fertilization or intracytoplasmatic sperm injection cycles with single embryo transfer were included...
October 2018: Fertility and Sterility
Thalia R Segal, Keewan Kim, Sunni L Mumford, James M Goldfarb, Rachel S Weinerman
OBJECTIVE: To assess the reproductive and neonatal outcomes of cycles in which donor oocyte embryos were transferred to gestational carriers compared to intended parent recipients. DESIGN: Retrospective cohort study. SETTING: Not applicable. PATIENT(S): Intended parent recipients and gestational carriers receiving donor oocyte embryos in 2014 in the United States. INTERVENTIONS(S): None. MAIN OUTCOMES MEASURE(S): Clinical pregnancy, live birth, miscarriage, plurality, prematurity, and birth weight from pregnancies conceived with donor oocyte embryos transferred to either a gestational carrier or an intended parent recipient...
October 2018: Fertility and Sterility
K Keano Pavlosky, Yoko Yamaguchi, Darren T Lerner, Andre P Seale
The Mozambique tilapia, Oreochromis mossambicus, is a teleost fish native to estuarine waters that vary in salinity between fresh water (FW) and seawater (SW). The neuroendocrine system plays a key role in salinity acclimation by directing ion uptake and extrusion in osmoregulatory tissues such as gill. While most studies with O. mossambicus have focused on acclimation to steady-state salinities, less is known about the ability of adult fish to acclimate to dynamically-changing salinities. Plasma osmolality, prolactin (PRL) levels, and branchial gene expression of PRL receptors (PRLR1 and PRLR2), Na+ /Cl- and Na+ /K+ /2Cl- co-transporters (NCC and NKCC), Na+ /K+ -ATPase (NKAα1a and NKAα1b), cystic fibrosis transmembrane conductance regulator (CFTR), and aquaporin 3 (AQP3) were measured in fish reared in FW and SW steady-state salinities, in a tidal regimen (TR) where salinities changed between FW and SW every 6 h, and in fish transferred from FW or SW to TR...
October 10, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
MeiFang Zeng, LiuMing Li
PURPOSES: Nowadays, an increasing number of studies have proposed single embryo transfer (SET), especially single blastocyst transfer (SBT). To compare the clinical outcomes of single fresh blastocyst transfer (BT) and single cryopreserved-thawed BT in patients undergoing in vitro fertilization or intracytoplasmic sperm injection (IVF/ICSI) cycles. METHODS: The PubMed, Embase, and Cochrane Library databases were searched from the start dates until February 2018...
October 10, 2018: Gynecological Endocrinology
Zhihui Xu, Lvhe Meng, Chengshuang Pan, Xia Chen, Xuefeng Huang, Haiyan Yang
This study aims to investigate whether oral contraceptive pills (OCP) pretreatment impairs pregnancy outcomes in polycystic ovary syndrome (PCOS) women undergoing GnRH agonist protocol. A total of 1025 couples underwent their first cycle of in vitro fertilization. Patients were divided into GnRH agonist protocol group (LP group) and OCP dual suppression GnRH agonist protocol group (OC-LP group). Logistic regressions were performed to estimate the risk factors affecting live birth following fresh embryo transfer between groups...
October 10, 2018: Gynecological Endocrinology
Cameron J Reid, Matthew Z DeMaere, Steven P Djordjevic
We recently identified clonal complex 10 (CC10) Escherichia coli as the predominant clonal group in two populations of healthy Australian food-production pigs. CC10 are highly successful, colonizing humans, food-production animals, fresh produce and environmental niches. Furthermore, E. coli within CC10 are frequently drug resistant and increasingly reported as human and animal extra-intestinal pathogens. In order to develop a high-resolution global phylogeny and determine the repertoire of antimicrobial-resistance genes, virulence-associated genes and plasmid types within this clonal group, we downloaded 228 publicly available CC10 short-read genome sequences for comparison with 20 porcine CC10 we have previously described...
October 10, 2018: Microbial Genomics
Y Ikemoto, K Kuroda, A Ochiai, S Yamashita, S Ikuma, S Nojiri, A Itakura, S Takeda
STUDY QUESTION: What is the prevalence of multiple pregnancy with zygotic splitting after single embryo transfer (SET)? SUMMARY ANSWER: The prevalence of multiple pregnancy with zygotic splitting after SET was 1.36%. WHAT IS KNOWN ALREADY: In 2008, the Japan Society of Obstetrics and Gynaecology (JSOG) recommended the adoption of SET to reduce multiple births. Since then, to improve the clinical pregnancy rate, elective SET using blastocyst transfer and frozen-warmed ET has increased...
October 8, 2018: Human Reproduction
Leonti Grin, Atara Indurski, Sophia Leytes, Mark Rabinovich, Shevach Friedler
Mid-trimester beta-human chorionic gonadotropin (BHCG) levels are considerably higher in pregnancies resulting from frozen embryo transfer (FET) compared with fresh (FRET), leading to a higher false positive rate in aneuploidy screening tests. We aimed to investigate the dynamics of BHCG increment and its predictive value for cycle outcome. A retrospective analysis of FRET and FET cycles. BHCG values on days 14 and 16 post embryo transfer were compared and stratified according to the number of sacs demonstrated on US scan at six weeks gestation, and pregnancy outcome (biochemical pregnancy, ectopic pregnancy, spontaneous abortion, and a singleton or twin birth)...
October 9, 2018: Gynecological Endocrinology
Marisol Tsui Chang, Luca Montanari, Prannoy Suraneni, W Jason Weiss
The goal of this method is to determine the chemical composition and electrical resistivity of cementitious pore solution expressed from a fresh paste sample. The pore solution is expressed from a fresh paste sample using a pressurized nitrogen gas system. The pore solution is then immediately transferred to a syringe to minimize evaporation and carbonation. After that, assembled testing containers are used for the X-ray fluorescence (XRF) measurement. These containers consist of two concentric plastic cylinders and a polypropylene film which seals one of the two open sides...
September 23, 2018: Journal of Visualized Experiments: JoVE
Dhafer Ahmed Alzahrani, Enas Jameel Albokhari
During 2013-2014, fresh material of Tetraena alba was collected from two localities in Saudi Arabia. Detailed morphological examination of these specimens revealed that there is a new variety of this species, for which the name T . alba var. arabica is proposed. Fresh material of Zygophyllum amblyocarpum was also collected from a different locality, and based on its characteristics is transferred to Tetraena with the new combination T . alba var. amblyocarpa . T . alba and its varieties are illustrated and morphological characters supplied to differentiate among them...
November 2017: Saudi Journal of Biological Sciences
Evangelos G Papanikolaou, Evi Timotheou, Petroula Tatsi, Hieronymus Janssens, Michael Grynberg, Apostolos Athanasiadis, Christina Zafeirati, Robert Najdecki, Stamatios Petousis
The efficacy of in vitro fertilization (IVF) for treating human infertility has only one final efficacy index and that is the achievement of a delivery. However, with the evolution of the freeze-all strategy, a new problem is arising for evaluating the performance of an embryological team. The aim of the study was to present a new representative index, combining fresh and frozen embryo transfer success rates. In this opinion article, apart from the effectiveness of managing fresh gametes and embryos, we wish to evaluate the efficacy of the processes of both freezing and thawing of the produced embryos...
January 2019: International Journal of Fertility & Sterility
Wenhui Hou, Gaohui Shi, Bing Cai, Chenhui Ding, Junli Song, Xiubing Zhang, Yanwen Xu
PURPOSE: This analysis was performed to evaluate the effects of intrauterine injection of human chorionic gonadotropin (hCG) before fresh embryo transfer (ET) on the outcomes of in vitro fertilization and intracytoplasmic sperm injection. METHODS: Randomized controlled trials (RCTs) were identified by searching electronic databases. The outcomes of live birth, clinical pregnancy, implantation, biochemical pregnancy, ongoing pregnancy, ectopic pregnancy, and miscarriage between groups with and without hCG injections were analyzed...
October 5, 2018: Archives of Gynecology and Obstetrics
Ensieh Shahrokh Tehrani Nejad, Fatemeh Bakhtiari Ghaleh, Bita Eslami, Fedyeh Haghollahi, Maryam Bagheri, Masoumeh Masoumi
Background: Both oral contraceptive pills (OCPs) and estradiol valerate (E2) have been used to schedule a gonadotropin-releasing hormone antagonist in vitro fertilization (IVF) cycles. Since the suppression of follicle-stimulating hormone by OCPs can stay 5-7 days after stopping the pills, it seems that starting the gonadotropin-releasing hormone (GnRH) after 6 days of pre-treatment discontinuation may be important in IVF outcomes. Objective: The aim of the present study was to determine the number of mature oocyte and pregnancy rate of three pretreatment methods for fresh embryo transfer cycles...
August 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
O Simon, B Gagnaire, V Sommard, S Pierrisnard, V Camilleri, L Carasco, R Gilbin, S Frelon
Uranium toxicity patterns for fish have been mainly determined under laboratory-controlled waterborne exposure conditions. Since, under in situ exposure conditions, fish can take up metals from water and diet, a waterborne U exposure experiment (20 µg.L-1 , 20d) was conducted in the laboratory to investigate transfer efficiency and target organ distribution in zebrafish, Danio rerio, as compared to combined waterborne (20 µg.L-1 ) and dietborne exposure (10.7 µg.g-1 ). 233 U was used as a specific uranium isotope tracer for dietborne exposure...
October 3, 2018: Environmental Toxicology and Chemistry
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