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Fresh transfer

T David Luo, Michael D Wigton, Benjamin W Berwick, Mario Khalil, Wayne A Chen, Ian R Smithson, Zhongyu Li
INTRODUCTION: The use of dorsal cutaneous branch of the ulnar nerve (DCBUN) transfer for median nerve (MN) sensory restoration has not been evaluated anatomically and histologically in humans. The purpose of this study was to evaluate the feasibility of DCBUN to MN transfer for sensory restoration with respect to nerve branch pattern, length, and fascicle count match. MATERIAL AND METHODS: Using seven fresh frozen cadaveric upper limb specimens, the DCBUN and its branch patterns, lengths, and sizes were recorded...
August 16, 2018: Clinical Anatomy
C W C Cheung, S Saravelos, T Y A Chan, D S Sahota, C C Wang, P W Chung, T C Li
OBJECTIVE: To ascertain whether stress biomarker and psychological indices of stress may predict both conception and miscarriage rates in women undergoing in-vitro fertilization/ intracytoplasmic sperm injection (IVF/ ICSI). DESIGN: Prospective observational study. SETTING: A university-affiliated tertiary hospital. POPULATION OR SAMPLE: Infertility women who were undergoing fresh or frozen IVF/ICSI cycles. METHODS: Subjects were recruited to (1) completed validated psychological questionnaires (visual analogue scale of stress, state trait anxiety inventory, perceived stress scale, fertility problem Inventory, Beck depression inventory, and general health questionnaire), at the time of embryo transfer (ET) and pregnancy testing (PT), and (2) provided saliva samples for alpha amylase (sAA) measurement before and after ET and at PT...
August 14, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
Maria-Theresia Gekenidis, Ulrich Schöner, Ueli von Ah, Mathias Schmelcher, Fiona Walsh, David Drissner
Environmental antibiotic-resistant bacteria (ARB) can be transferred to humans through foods. Fresh produce in particular is an ideal vector due to frequent raw consumption. A major contamination source of fresh produce is irrigation water. We hypothesized that water quality significantly affects loads of ARB and their diversity on fresh produce despite various other contamination sources present under agricultural practice conditions. Chive irrigated from an open-top reservoir or sterile-filtered water (control) was examined...
August 7, 2018: FEMS Microbiology Ecology
Tarak Agrebi Moumni Chouari, Karen Lindsay, Ellen Bradshaw, Simon Parson, Lucy Watson, Jamil Ahmed, Alain Curnier
Background: Performing microsurgery requires a breadth and depth of experience that has arguably been reduced as result of diminishing operating exposure. Fresh frozen cadavers provide similar tissue handling to real-time operating; however, the bloodless condition restricts the realism of the simulation. We describe a model to enhance flap surgery simulation, in conjunction with qualitative assessment. Methods: The fresh frozen cadaveric limbs used in this study were acquired by the University...
2018: European Journal of Plastic Surgery
Lukas Cerveny, Zuzana Ptackova, Martina Ceckova, Rona Karahoda, Sara Karbanova, Lucie Jiraskova, Susan L Greenwood, Jocelyn D Glazier, Frantisek Staud
Abacavir is a preferred antiretroviral drug for preventing mother-to-child HIV transmission; however, mechanisms of its placental transfer have not been satisfactorily described to date. Because abacavir is a nucleoside-derived drug, we hypothesized that the nucleoside transporters, ENTs (SLC29A) and/or Na+ dependent CNTs (SLC28A), may play a role in its passage across the placenta. To test this hypothesis, we performed uptake experiments using BeWo cell line, human fresh villous fragments and microvillous plasma membrane (MVM) vesicles...
August 10, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
Van Huong Do, Sally Catt, German Amaya, Madeline Batsiokis, Simon Walton, Andrew W Taylor-Robinson
The present study was conducted in cattle to test the null hypothesis that the pregnancy rate of recipient females is similar when in vitro-derived embryos are transferred either fresh (non-vitrified) or after being subjected to vitrification. Cumulus-oocyte complexes, collected twice (6 weeks apart) from 10 donor cows were matured in vitro and inseminated with frozen-thawed sperm from a single proven bull per donor collection. Cleaved embryos were cultured in vitro until day 7 and any resulting blastocysts were graded for stage [early (unexpanded), advanced (expanded, hatching, hatched)] and/or quality and either discarded (poor quality), or, if deemed suitable, transferred fresh or vitrified for later warming and transfer...
August 1, 2018: Theriogenology
S P Wraight, S Galaini-Wraight, L A Castrillo, M H Griggs, L M Keith, T K Matsumoto
The insect pathogenic fungus Hirsutella eleutheratorum was first reported as a pathogen of coffee berry borer (CBB) Hypothenemus hampei in Colombia in 1993. A similar CBB pathogen identified as Hirsutella sp. was reported also from Colombia in 2007; attempts at isolation and in vitro culture of this fungus were unsuccessful. During 2016 and 2017 on the island of Hawai'i, extensive sampling of CBB populations was conducted in coffee fields treated with Beauveria bassiana-based biopesticides and in untreated fields...
August 7, 2018: Journal of Invertebrate Pathology
Dominique Chabot, Antoine Lewin, Lionel Loubaki, RenÉe Bazin
BACKGROUND: We recently showed that transient warming effects decreased the functional and adhesion properties of mesenchymal stromal cells (MSC) while post-thaw viability remained high. In an attempt to better predict functional impairment of cryopreserved MSC, we further analysed the correlation between viability, immunosuppressive activity and adhesion of cells exposed or not to warming events. METHODS: MSC prepared from six umbilical cords were frozen to -130°C and immediately transferred in a dry ice container or exposed to room temperature for 2 to 10 min (warming events) prior to storage in liquid nitrogen...
August 6, 2018: Cytotherapy
Song-Po Pan, Kuang-Han Chao, Chu-Chun Huang, Ming-Yih Wu, Mei-Jou Chen, Chin-Hao Chang, Jehn-Hsiahn Yang, Yu-Shih Yang, Shee-Uan Chen
Previous studies indicated that progesterone can be withdrawn at the time of the first positive β-hCG test without compromising the clinical pregnancy outcome in normal ovarian responder. However, the effect of early stop of progesterone supplementation for patients with poor ovarian response (POR) has not been investigated. This study retrospectively collected data from patients with POR in 156 IVF/ICSI fresh embryo transfer (ET) cycles in single tertiary center from January 2010 to June 2016. All the patients met ESHRE consensus, the Bologna criteria, of POR and had hCG injection for luteal phase support (LPS) on day 2, 5 and 8 after ovum pick-up...
2018: PloS One
Lingmin Hu, Jiangbo Du, Hong Lv, Jing Zhao, Mengxi Chen, Yifeng Wang, Fang Wu, Feng Liu, Xiaojiao Chen, Junqiang Zhang, Hongxia Ma, Guangfu Jin, Hongbing Shen, Li Chen, Xiufeng Ling, Zhibin Hu
BACKGROUND: Pregnancies following assisted reproductive technology (ART) may have elevated potential risk of pregnancy loss (PL) when compared to natural conception. However, rare studies comprehensively analyzed the IVF/ICSI cycle-dependent factors for loss of clinical pregnancy. Therefore, we aimed to determine the ART subgroup-specific risks of PL throughout pregnancy and explore different risk factors for early miscarriage and late miscarriage among pregnancies conceived through ART...
August 7, 2018: Reproductive Biology and Endocrinology: RB&E
J A Leijdekkers, M J C Eijkemans, T C van Tilborg, S C Oudshoorn, D J McLernon, S Bhattacharya, B W J Mol, F J M Broekmans, H L Torrance
STUDY QUESTION: Are the published pre-treatment and post-treatment McLernon models, predicting cumulative live birth rates (LBR) over multiple complete IVF cycles, valid in a different context? SUMMARY ANSWER: With minor recalibration of the pre-treatment model, both McLernon models accurately predict cumulative LBR in a different geographical context and a more recent time period. WHAT IS KNOWN ALREADY: Previous IVF prediction models have estimated the chance of a live birth after a single fresh embryo transfer, thereby excluding the important contribution of embryo cryopreservation and subsequent IVF cycles to cumulative LBR...
August 6, 2018: Human Reproduction
Willem Verpoest, Catherine Staessen, Patrick M Bossuyt, Veerle Goossens, Gheona Altarescu, Maryse Bonduelle, Martha Devesa, Talia Eldar-Geva, Luca Gianaroli, Georg Griesinger, Georgia Kakourou, Georgia Kokkali, Jana Liebenthron, Maria-Cristina Magli, Monica Parriego, Andreas G Schmutzler, Monica Tobler, Katrin van der Ven, Joep Geraedts, Karen Sermon
STUDY QUESTION: Does preimplantation genetic testing for aneuploidy (PGT-A) by comprehensive chromosome screening (CCS) of the first and second polar body to select embryos for transfer increase the likelihood of a live birth within 1 year in advanced maternal age women aged 36-40 years planning an ICSI cycle, compared to ICSI without chromosome analysis? SUMMARY ANSWER: PGT-A by CCS in the first and second polar body to select euploid embryos for transfer does not substantially increase the live birth rate in women aged 36-40 years...
August 6, 2018: Human Reproduction
Garyfalia Karponi, Penelope-Georgia Papayanni, Fani Zervou, Asimina Bouinta, Achilles Anagnostopoulos, Evangelia Yannaki
Stable gene marking and effective engraftment of gene-modified CD34+ hematopoietic stem cells is a prerequisite for gene therapy success, but may be challenged by the inevitable cryopreservation of the final product, prior to extensive quality assurance testing. We investigated the β-globin gene transfer potency in fresh and cryopreserved CD34+ cells from mobilized patients with β-thalassemia, as well as the qualitative impact of repeated freeze/thaw cycles on the functionality of cultured and unmanipulated CD34+ cells in terms of engrafting capacity in a xenotransplantation model, under partial myeloablation...
August 4, 2018: Human Gene Therapy Methods
Eleanor Spielman-Sun, Enzo Lombi, Erica Donner, Astrid Avellan, Barbara Etschmann, Daryl L Howard, Gregory V Lowry
Utilization of nanoparticles (NP) in agriculture as fertilizers or pesticides requires an understanding of the NP properties influencing their interactions with plant roots. To evaluate the influence of the solubility of Cu-based NP on Cu uptake and NP association with plant roots, wheat seedlings were hydroponically exposed to 1 mg/L of Cu NPs with different solubilities (CuO, CuS, and Cu(OH)2 ) for 1 h, then transferred to a Cu free medium for 48 h. Fresh, hydrated roots were analyzed using micro X-ray fluorescence (μ-XRF) and imaging fluorescence X-ray absorption near edge spectroscopy (XANES imaging) to provide laterally resolved distribution and speciation of Cu in roots...
August 5, 2018: Environmental Science & Technology
Nima Khavanin, Edward H Davidson, Ryan M Smith, Alexandra Macmillan, Patrick Byrne, Amir H Dorafshar
Methods for harvest of the temporomandibular joint (TMJ) for transplantation may involve several anatomic levels. The authors aim to assess the feasibility and identify challenges with 2 such methods, resuspending the donor condyles from the recipient glenoid fossae and en bloc harvest of the joint and surrounding temporal bone with plate-fixation to the recipient skull base. Two mock face transplantations were carried out using 4 fresh cadavers. Computed tomography imaging was obtained before and after the procedures to assess the technical success of each method...
August 2, 2018: Journal of Craniofacial Surgery
Keke Guo, Mijun Peng, Chaoying Tong, Shuyun Shi, Yuge Liu
Extraction is the most important step in the purification of bioactive compounds from natural products. This study interpreted a simple online extraction strategy coupled with high-speed counter-current chromatography for efficient extraction and purification of bioactive components from solid natural products. For online extraction strategy, 1.0 g of gound Mangnolia officinalis or Piper nigrum was loaded into a guard column, which was then positioned on the manual injection valve instead of the sample loop...
August 2, 2018: Journal of Separation Science
Raul Eduardo Piña-Aguilar, Claudia González-Ortega, Anna Calull-Bago, María Cristina Lanuza-López, Patricia Cancino-Villarreal, Ana Mireya Gutiérrez-Gamiño, Antonio Martin Gutiérrez-Gutiérrez
Background: Hypohidrotic ectodermal dysplasia (HED) is a genetic skin condition presenting as hypohidrosis, hypodontia, and hypotrichosis, resulting in an important burden for affected families. The most common form of HED has an X-linked inheritance and female carriers have the option of prenatal or preimplantation genetic testing (PGT) to avoid transmission of the disease. A combined PGT for a mutation in EDA gene and aneuploidies in a Mexican carrier of X-linked HED is reported. Materials and Methods: Ovarian stimulation and assisted reproduction procedures were performed in a private academic medical center...
2018: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
Joseph C Grim, Tobin E Brown, Brian A Aguado, Douglas A Chapnick, Alexandrea L Viert, Xuedong Liu, Kristi S Anseth
Biomolecule-functionalized hydrogels have emerged as valuable cell culture platforms to recapitulate the mechanical and biochemical properties of the extracellular niche. The typical strategy to functionalize hydrogels with biomolecules involves directly tethering them to the hydrogel backbone resulting in a static material. Thus, this approach fails to capture the dynamic changes in biomolecule composition that occur during biological processes or that may be required for regenerative medicine applications...
July 25, 2018: ACS Central Science
Yan Zhao, Luyi Yang, Yunxing Zuo, Zhibo Song, Fang Liu, Ke Li, Feng Pan
Employing conductive binders in silicon (Si) anode have been considered as a fundamental solution to the pulverization of Si particles. Therefore, it is still a great challenge to improve the charge transfer capability of the conductive binder. Herein, a copolymer (PFPQ-COONa) is synthesized, characterized and electrochemically tested as conductive binder for Si anode. It is found that PFPQ-COONa exhibits not only excellent cycling stability, but also satisfactory rate performance with relatively high areal loading, which outperforms currently reported single component conductive binders...
July 30, 2018: ACS Applied Materials & Interfaces
Chao Xu, Fang-Fang Zhang, Hong-Chang Li, Miao-Miao Wang, Yue-Ting Zhu, Wen-Jie Jiang, Yue Wang, Hao-Bo Zhang, Rong Tang, Gang Ma, Jun-Hao Yan
Background: The 47,XYY syndrome could result in fertility problems. However, seldom studies reported comprehensive researches on the embryonic development and pregnancy outcomes of these patients. This study aimed to evaluate the clinical outcomes of nonmosaic 47,XYY patients performed with fluorescent in situ hybridization (FISH) and preimplantation genetic diagnosis (PGD) treatment. Methods: This was a retrospective study. Between January 2012 and May 2017, 51 infertile males with nonmosaic 47,XYY syndrome underwent FISH-PGD were included in the study...
August 5, 2018: Chinese Medical Journal
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