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granulation tissue

O V Payushina, N N Butorina, O N Sheveleva, E I Domaratskaya
We studied the effect of mesenchymal stromal cells and conditioned media on healing of full-thickness skin wound in rats. Cell transplantation to the wound bed did not accelerate wound closing and had no effect on the severity of inflammation, but increased vasculariza - tion of the granulation tissue in 14 days after injury. After injection of conditioned medium to the wound, less pronounced inflammation or enhanced epithelialization was observed. The angiogenic effect was observed only after repeated administration of conditioned medium and was associated with slower regeneration, probably due to skin traumatization by repeated injections...
August 18, 2018: Bulletin of Experimental Biology and Medicine
Ayşegül Mendi, Beyza Gökçınar Yağcı, Nurdan Saraç, Mustafa Kızıloğlu, Aysel Uğur, Duygu Uçkan, Derviş Yılmaz
The aim of this study is to demonstrate and compare the differentiation, proliferation, migration and inflammatory behavior of dental pulp- and bone marrow-derived mesenchymal stem cells (DP-MSCs and BM-MSCs) in response to a Hypericum perforatum ethanol extract. Using xCELLigence, a real-time monitoring system, a dose of 10 µg/mL was found to be the most efficient concentration for vitality. The IC50 values and doubling time were calculated. The results showed that H. perforatum L. was able to accelerate osteogenic differentiation in DP-MSCs, but calcium granulation was impaired in BM-MSCs...
August 17, 2018: Cells, Tissues, Organs
Yang Xu, Jie Xu, Yanfang Yang, Lei Zhu, Xubin Li, Weipeng Zhao
BACKGROUND/AIMS: The chondroitin sulfate proteoglycan serglycin (SRGN), a hematopoietic cell granule proteoglycan, has been implicated in promoting tumor metastasis; however, the underlying mechanisms remain to be elucidated. The present study aimed to investigate the SRGN gene expression and its regulation as downstream signaling of hypoxia-inducible transcription factor 1 alpha (HIF-1α) in colorectal cancer (CRC) cells and tissues. METHODS: The expression of SRGN was analyzed in CRC specimens for its correlation with progression and metastasis...
August 17, 2018: Cellular Physiology and Biochemistry
K Lalrinzuali, M Vabeiryureilai, Ganesh Chandra Jagetia
ETHNOPHARMACOLOGICAL RELEVANCE: The Oroxylum indicum is used traditionally to treat fever, colic, stomach ulcers, constipation, indigestion, intestinal worms, strangury, asthma, cough, hiccough, diarrhea, dysentery and wounds by the herbal healers of Mizoram and it is also part of Ayurvedic formulations. AIMS OF THE STUDY: The wound healing activity of Oroxylum indicum has not been investigated therefore the present study was undertaken to evaluate the ability of different concentrations of ethanol extract of stem bark of Oroxylum indicum in the deep dermal excision wounds of mice...
August 16, 2018: Journal of Ethnopharmacology
Mohamed Gadelkarim, Abdelrahman Ibrahim Abushouk, Esraa Ghanem, Ali Mohamed Hamaad, Anas M Saad, Mohamed M Abdel-Daim
With the increasing global prevalence of diabetes mellitus, a significant rise in the number of patients suffering from non-healing wounds is expected. However, available treatments, such as revascularization surgery and foot care education are often insufficient to ensure satisfactory wound healing. One therapeutic strategy that has been identified as particularly promising utilizes adipose-derived stem cells (ADSCs). Through a comprehensive literature search of published and ongoing studies, we aimed to provide an overview of the experimental basis, the scientific background, and advances in the delivery of ADSCs for treating non-healing diabetic wounds...
August 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Corrie L Gallant-Behm, Joseph Piper, Brent A Dickinson, Christina M Dalby, Linda A Pestano, Aimee L Jackson
There is a strong unmet need for new therapeutics to accelerate wound healing across both chronic and acute indications. It is well established that local tissue hypoxia, vascular insufficiency and/or insufficient angiogenesis contribute to inadequate wound repair in the context of diabetic foot ulcers as well as to other chronic wounds such as venous stasis and pressure ulcers. microRNA-92a-3p (miR-92a) is a potent anti-angiogenic miRNA whose inhibition has led to increases in angiogenesis in multiple organ systems, resulting in an improvement in function following myocardial infarction, limb ischemia, vascular injury and bone fracture...
August 17, 2018: Wound Repair and Regeneration
Stefania Recalcati, Elena Gammella, Paolo Buratti, Andrea Doni, Achille Anselmo, Massimo Locati, Gaetano Cairo
Iron recycling by macrophages is essential for erythropoiesis, but may be also relevant for iron redistribution to neighbouring cells at the local tissue level. Using mice with iron retention in macrophages due to targeted inactivation of the iron exporter ferroportin, we investigated the role of macrophage iron release in hair follicle cycling and wound healing, a complex process leading to major clinical problems, if impaired. Genetic deletion of ferroportin in macrophages resulted in iron deficiency and decreased proliferation in epithelial cells, which consequently impaired hair follicle growth and caused transient alopecia...
August 16, 2018: Haematologica
Yoshihiko Esu, Yukiko Iino, Maria Masuda, Hiromi Kanazawa, Naohiro Yoshida
OBJECTIVE: We classified eosinophilic otitis media (EOM) into three grades (G1-G3) based on the middle ear mucosal thickness and aimed to establish a treatment strategy for EOM based on these pathological categories. STUDY DESIGN: Retrospective cohort study. SETTING: Tertiary referral center. PATIENTS: We evaluated 136 ears of 68 patients (38 women, 30 men; average age, 56.1 yr; range, 32-80 yr) with bilateral EOM, including 14 surgically treated ears...
September 2018: Otology & Neurotology
Ernest Adeghate, Zulqarnain Saeed, Crystal D'Souza, Saeed Tariq, Huba Kalász, Kornélia Tekes, Ernest A Adeghate
Nociceptin (NC), also known as Orphanin FQ, is a brain peptide involved in the regulation of pain, but its role in the endocrine pancreas is poorly understood. The present study examines the pattern of distribution of NC and its effect on insulin and glucagon secretion after the onset of diabetes mellitus (DM). Male Wistar rats weighing 150-200 g were made diabetic with streptozotocin (60 mg/kg body weight, intraperitoneally). Four weeks after the induction of DM, pancreatic tissues were retrieved and processed for immunofluorescence, immunoelectron microscopy, and insulin and glucagon secretion...
August 15, 2018: Cell and Tissue Research
Zhoupeng Wu, Xiaoyan Liu, Ding Yuan, Jichun Zhao
Venous lower limb ulcers are very common and affect ~1% of the general population. The human acellular amniotic membrane (HAAM), which is isolated from the amniotic membrane (AM) via excluding the majority of cellular components, has lower antigenicity than the AM. The aim of the present study was to evaluate the effectiveness and safety of the HAAM, adopted to treat venous ulcers (VUs) of the lower extremities. The HAMM was isolated from the AM by the Stem Cells and Tissue Engineering laboratory at the West China Hospital of Sichuan University (Chengdu, China)...
August 2018: Experimental and Therapeutic Medicine
Philip Wertz
In the mid-1950s and 1960s, transmission electron microscopes became widely available, leading to many studies of the ultrastructure of various tissues including the epidermis. Most of these studies involved tissue fixation with formaldehyde and postfixation with osmium tetroxide. A few studies employed freeze-fracture electron microscopy. One set of these studies identified a small organelle variously called lamellar granules (LGs), lamellar bodies, membrane-coating granules, cementsomes, and Odland bodies...
August 15, 2018: Skin Pharmacology and Physiology
Maria Steblyanko, Nadia Anikeeva, Marcus Buggert, Michael R Betts, Yuri Sykulev
The current understanding of the dynamics and structural features of T-cell synaptic interfaces has been largely determined through the use of glass-supported planar bilayers and in vitro-derived T-cell clones or lines1 , 2 , 3 , 4 . How these findings apply to the primary human T cells isolated from blood or lymphoid tissues is not known, partly due to significant difficulties in obtaining a sufficient number of cells for analysis5 . Here we address this through the development of a technique exploiting multichannel flow slides to build planar lipid bilayers containing activating and adhesion molecules...
July 30, 2018: Journal of Visualized Experiments: JoVE
Andrew Applewhite, Saeed A Chowdhry, Michael Desvigne, Allen Gabriel, Rosemary Hill, Mary Anne Obst, Dawn Shepherd, Marcus Speyrer, Terry Treadwell, Lindsey Waddell
The increase in wound prevalence means more patients with wounds are being transferred through care settings than ever before. Although the goals of therapy may be the same in both settings, wound care therapies and dressings differ in availability and appropriateness for each setting. Negative pressure wound therapy (NPWT) modalities and oxidized regenerated cellulose (ORC)/collagen (C)/silver-ORC dressings are available in both inpatient and outpatient care settings, but (to-date) lack comprehensive information regarding best practices in transitioning use of these therapies between various care settings...
August 2018: Wounds: a Compendium of Clinical Research and Practice
Yu Kuroiwa, Takahiro Niikura, Sang Yang Lee, Keisuke Oe, Takashi Iwakura, Tomoaki Fukui, Tomoyuki Matsumoto, Takehiko Matsushita, Kotaro Nishida, Ryosuke Kuroda
PURPOSE: This study investigated whether Escherichia coli-derived bone morphogenetic protein (BMP)-2 (E-BMP-2) adsorbed onto β-tricalcium phosphate (β-TCP) granules can induce bone regeneration in critical-size femoral segmental defects in rabbits. METHODS: Bone defects 20 mm in size and stabilized with an external fixator were created in the femur of New Zealand white rabbits, which were divided into BMP-2 and control groups. E-BMP-2-loaded β-TCP granules were implanted into defects of the BMP-2 group, whereas defects in the controls were implanted with β-TCP granules alone...
August 10, 2018: International Orthopaedics
María Fernández-Ibieta
Case Report  An 11-month-old female infant presented on the first postoperative (PO) day following an elective pyeloplasty, a dark bluish erythema of her lumbotomy wound, plus a satellite lesion of the same characteristics. Fever and sepsis developed, and despite broad spectrum antibiotics (meropenem and vancomycin) were started, a diagnosis of necrotizing soft-tissue infection (NSTI or necrotizing fasciitis) was established. Surgical debridement of both lesions was performed on day 3 PO, and a surgical contamination (ring retractor blade) was suspected, due to the particular geography of the lesion...
July 2018: Surgery Journal
Richard J Bodnar, Tianbing Yang, Lora H Rigatti, Fang Liu, Alexander Evdokiou, Sandeep Kathju, Latha Satish
BACKGROUND: Pericytes have been shown to have mesenchymal stromal cell-like properties and play a role in tissue regeneration. The goal of this study was to determine whether the addition of a pericyte sheet to a full-thickness dermal wound would enhance the healing of an acute wound. METHODS: Human muscle-derived pericytes and human dermal fibroblasts were formed into cell sheets, then applied to full-thickness excisional wounds on the dorsum of nu/nu mice. Histology was performed to evaluate epidermal and dermal reformation, inflammation and fibrosis...
August 6, 2018: Cytotherapy
M Cavaliere, Antonella Miriam Di Lullo, E Cantone, G Scala, A Elefante, C Russo, L Brunetti, G Motta, M Iengo
PURPOSE: To diagnose cholesteatoma when it is not visible through tympanic perforation, imaging techniques are necessary. Recently, the combination of computed tomography and magnetic resonance imaging has proven effective to diagnose middle ear cholesteatoma. In particular, diffusion weighted images have integrated the conventional imaging for the qualitative assessment of cholesteatoma. Accordingly, the aim of this study was to obtain a quantitative analysis of cholesteatoma calculating the apparent diffusion coefficient value...
August 7, 2018: European Archives of Oto-rhino-laryngology
Jaime Castro-Núñez, Trent Clifton, Joseph Van Sickels
Orbital blow-out fractures are very common. Goals of surgical treatment are to reestablish form and function with excellent cosmetic results. Materials used for orbital floor repair include autologous grafts and a variety of alloplastic materials. In this article, we present a case of subacute granulation tissue of the fornix that developed as a foreign body reaction 4 months after placing a resorbable implant to repair an orbital floor fracture. The implant was removed and the patient continued to heal uneventfully...
September 2018: Craniomaxillofacial Trauma & Reconstruction
Guifang Shi, Wenting Chen, Yu Zhang, Xiaomei Dai, Xinge Zhang, Zhongming Wu
The patients with diabetic wounds have deficient local and systemic cellular immunity. Herein, a new silver nanoparticles-contained hydrogel with antifouling property was developed for enhancing immune response in diabetic wound healing. The antifouling property was obtained by adjusting the composition of cationic chitosan and anionic dextran to approach to zero charge. Furthermore, this hybrid hydrogel showed long-lasting and broad-spectrum antibacterial activity. The rapid wound contraction was observed after the treatment of hydrogel, which suggested its superior healing activity to promote fibroblast migration, granulation tissue formation and angiogenesis...
August 7, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Yukio Yoshioka, Hirotaka Nakatao, Tomoaki Hamana, Atsuko Hamada, Taku Kanda, Koichi Koizumi, Shigeaki Toratani, Tetsuji Okamoto
INTRODUCTION: Suture granuloma is a benign tumor that develops because of the presence of surgical suture materials. It commonly occurs several years after different types of surgeries. Here we report a case involving a 64-year-old man who underwent head and neck surgery for oral squamous cell carcinoma and developed multiple suture granulomas mimicking tumor recurrence in the radiation field just a few days after the completion of adjuvant chemoradiation therapy. PRESENTATION OF CASE: The patient underwent surgery for lymph node metastasis in the neck at 6 months after the resection of primary oral squamous cell carcinoma...
July 27, 2018: International Journal of Surgery Case Reports
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