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Michael J G Milevskiy, Gurveen K Sandhu, Anna Wronski, Darren Korbie, Brooke L Brewster, Annette Shewan, Stacey L Edwards, Juliet D French, Melissa A Brown
Depletion of BRCA1 protein in mouse mammary glands results in defects in lactational development and increased susceptibility to mammary cancer. Extensive work has focussed on the role of BRCA1 in the normal breast and in the development of breast cancer, the cell of origin for BRCA1 tumours and the protein-coding genes altered in BRCA1 deficient cells. However, the role of non-coding RNAs in BRCA1-deficient cells is poorly understood. To evaluate miRNA expression in BRCA1 deficient mammary cells, RNA sequencing was performed on the mammary glands of Brca1 knockout mice...
September 11, 2018: Oncotarget
Brendan M Browne, Kristian D Stensland, Chintan K Patel, Travis Sullivan, Eric J Burks, David Canes, Jay D Raman, Joshua Warrick, Kimberly M Reiger-Christ
OBJECTIVE: To evaluate microRNA biomarkers for upper tract urothelial carcinoma (UTUC) to improve risk stratification. METHODS: MicroRNA was isolated from 157 radical nephroureterectomy (RNU) specimens from two institutions. The relative expression of miRNA was examined for high grade versus low grade tumors as well as muscle invasive versus non-muscle invasive tumors. Recurrence free survival (RFS) and overall survival (OS) were also stratified using relative expression of specific miRNA...
October 10, 2018: Urology
Pradeep Kumar, Reben Raeman, Daniel M Chopyk, Tekla Smith, Kiran Verma, Yunshan Liu, Frank A Anania
Adiponectin inhibits hepatic stellate cell (HSC) activation and subsequent development of liver fibrosis via multiple mechanisms. Phosphatase and tensin homolog deletion 10 (PTEN) plays a crucial role in suppression of HSC activation, but its regulation by adiponectin is not fully understood. Here, we investigated the effect of adiponectin on PTEN in LX-2 cells, a human cell line and examined the underlying molecular mechanisms involved in adiponectin-mediated upregulation of PTEN activity during fibrosis. PTEN expression was found to be significantly reduced in the livers of mice treated with CCl4 , whereas its expression was rescued by adiponectin treatment...
October 2018: Biochimica et biophysica acta. Molecular basis of disease
Jia Liu, Yanrong Wen, Hui He, Hong-Yuan Chen, Zhen Liu
MicroRNAs (miRNAs) regulate the expression of mRNAs in cells. The determination of subcellular miRNAs in single living cells is essential for understanding the subcellular localizations and functions of miRNAs as well as the microheterogeneity of cells. However, current approaches fail either to keep the cells alive or maintain their original cellular composition or are unable to provide subcellular resolution. Herein, we presented a new approach, called plasmonic affinity sandwich assay (PASA), for rapid and ultrasensitive probing of trace cytoplasmic and nuclear miRNAs in single living cells...
September 28, 2018: Chemical Science
João V Esteves, Caio Y Yonamine, Danilo C Pinto-Junior, Frederico Gerlinger-Romero, Francisco J Enguita, Ubiratan F Machado
The reduced expression of solute carrier family 2, facilitated glucose transporter member 4 (GLUT4) and hexokinase-2 (HK2) in skeletal muscle participates in insulin resistance of diabetes mellitus (DM). MicroRNAs (miRNAs) have emerged as important modulators of mRNA/protein expression, but their role in DM is unclear. We investigated miRNAs hypothetically involved in GLUT4/HK2 expression in soleus muscle of type 1 diabetes-like rats. In silico analysis revealed 651 miRNAs predicted to regulate solute carrier family 2 member 4 ( Slc2a4 ) mRNA, several of them also predicted to regulate Hk2 mRNA, and 16 miRNAs were selected for quantification...
2018: Frontiers in Endocrinology
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Saad Wan Zuhainis, Mohammad Navaderi, Rosfarizan Mohamad
Background: In recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression. Objectives: The aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties...
2018: International Journal of Nanomedicine
Faqing Liang, Meng Yang, Ning Tong, Jinju Fang, Yanbin Pan, Jianmin Li, Xiao Zhang
Breast Cancer (BC) is one of the most common primary malignant tumors, which is life threatening. Previous studies have demonstrated that microRNAs (miRNA) may regulate or affect the incidence of BC. However, results of these studies are inconsistent, due to factors including the different sequencing platforms and sample selection methods used. To explore the key miRNAs involved in the pathogenesis of BC, and to use these miRNAs to monitor the tumor progression of BC, a systematic review was performed on the previous studies examining BC miRNA; the function of the target genes that were modulated by these key miRNAs were also analyzed...
October 2018: Oncology Letters
Zhi Huang, Lu Lu, Tianpeng Jiang, Shuai Zhang, Yaping Shen, Zhu Zheng, Ansu Zhao, Rui Gao, Rui Li, Shi Zhou, Jing Liu
The present study aimed to determine whether an miRNA (miR)-29b inhibitor protected against cerebral ischemia/reperfusion (I/R) injury in vitro and to investigate the underlying mechanisms. As a model for induced cerebral IR injury, N2a cells were exposed to an oxygen-glucose deprivation/reoxygenation (OGD/R) environment. Using this model, it was demonstrated that miR-29b was significantly upregulated compared with cells in a normal environment. The interactions between miR-29b and myeloid cell leukemia sequence (MCL)-1 were then investigated using dual-luciferase assays, revealing a strong regulation of MCL-1 through the 3'untranslated region...
October 2018: Experimental and Therapeutic Medicine
Qian-Ru Sun, Xiong Zhang, Kun Fang
BACKGROUND Phenotypic switch of vascular smooth muscle cells (VSMCs) participates in the etiology of various vascular diseases. It has been proved that microRNAs (miRNAs) serve as crucial regulators of functions of VSMCs. This study aimed to discover how miR-29b regulates the transformation of VSMCs phenotypes in mice. MATERIAL AND METHODS Primary VSMCs of aorta in mice were cultured in DMEM medium. A series of experiments involving transfection of oligonucleotides in cultured VSMCs, quantitative reverse transcription PCR (qRT-PCR), luciferase reporter assay, and Western blotting analysis were performed in this study...
September 19, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Manjari Baluni, Sneha Ghildiyal, Dharamveer Singh, D Himanshu Reddy, Rashmi Kumar, Tapan N Dhole
BACKGROUND: Japanese encephalitis virus (JEV) is a neurotropic mosquito-borne Flavivirus, mainly prevalent in Asia. It is the most important causative agent of acute viral encephalitis in humans. Recently, micro RNAs are discovered as a key regulator of inflammatory and immune responses in various diseases including neurological and viral infections. Thus, this study was proposed to check whether changes in cellular miRNA expression due to JE virus infection, can be detected in circulation which would be helpful in diagnosis and treatment...
October 15, 2018: Journal of Neuroimmunology
Steven D Scoville, Ansel P Nalin, Luxi Chen, Li Chen, Michael Zhang, Kathleen McConnell, Susana Beceiro Casas, Gabrielle Ernst, Abd Al-Rahman Traboulsi, Naima Hashi, Monica Williams, Xiaoli Zhang, Tiffany Hughes, Anjali Mishra, Don M Benson, Jennifer N Saultz, Jianhua Yu, Aharon G Freud, Michael A Caligiuri, Bethany L Mundy-Bosse
Acute myeloid leukemia (AML) can evade the mouse and human innate immune system by suppressing natural killer (NK) cell development and NK cell function. This is driven in part by the overexpression of microRNA (miR)-29b in the NK cells of AML patients, but how this occurs is unknown. In the current study, we demonstrate that the transcription factor aryl hydrocarbon receptor (AHR) directly regulates miR-29b expression. We show that human AML blasts activate the AHR pathway and induce miR-29b expression in NK cells, thereby impairing NK cell maturation and NK cell function, which can be reversed by treating NK cells with an AHR antagonist...
August 29, 2018: Blood
Yasutaka Yamada, Sho Sugawara, Takayuki Arai, Satoko Kojima, Mayuko Kato, Atsushi Okato, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Seki
OBJECTIVES: To identify key oncogenes and proteins that are controlled by the microRNA miR-29 family (miR-29a, miR-29b and miR-29c) in renal cell carcinoma pathogenesis. METHODS: Genome-wide gene expression and in silico database analyses were carried out. The Cancer Genome Atlas database was used to investigate the clinical significance of gene expression data in renal cell carcinoma patients. Loss-of-function assays were applied to investigate the function of target genes...
August 28, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
Rupal Ojha, Raj Nandani, Rajan Kumar Pandey, Amit Mishra, Vijay Kumar Prajapati
The endogenic microRNAs (miRNA) are evolutionary, conserved, and belong to a group of small noncoding RNAs with a stretch of 19-24 nucleotides. The miRNAs play an indispensable role in gene modulation at the posttranscriptional level, inclusive of stem-cell differentiation, embryogenesis, hematopoiesis, metabolism, immune responses, or infections. The miRNAs secreted from the cells and their presence in the biological fluids signifies the regulatory role of circulating miRNAs in the pathogenesis. The phenomenal expression levels of circulating miRNAs in serum or plasma during infection makes them the potential therapeutic biomarkers for the diagnosis of assorted human infectious diseases...
August 26, 2018: Journal of Cellular Physiology
Chuanan Zhu, Xiumei Hou, Jiabin Zhu, Chunxiao Jiang, Wei Wei
This study aimed to investigate the expression of miR-30c and miR-29b in prostate cancer (PCa) and its clinical significance. The expression of miR-30c and miR-29b was detected by RT-qPCR in 187 cases of PCa and their adjacent tissues. Combined with clinical information, the correlation between the expression of miR-29b and miR-30c and the clinical features of PCa was analyzed, and ROC curve was plotted. The expression of miR-30c and miR-29b detected by RT-qPCR showed that the expression of miR-29b and miR-30c in PCa tissues was significantly lower than that in adjacent cancerous tissues (p<0...
September 2018: Oncology Letters
Wei Yan, Wenchao Yang, Zhongcai Liu, Guoyang Wu
Background and objective: Metastasis is the major cause of cancer-related deaths in patients with colon cancer, however, the exact molecular mechanism is unclear. MicroRNAs (miRNAs) play an important role in the pathogenesis and progression of cancer. Therefore, in this study, we aimed to identify differentially expressed miRNAs in two colon carcinoma cell lines: SW480, derived from primary colon carcinoma and SW620, derived from lymph node metastasis, which were obtained from the same patient...
2018: OncoTargets and Therapy
Hong Xue, Guangyuan Zhang, Aron M Geurts, Kristie Usa, David M Jensen, Yong Liu, Michael E Widlansky, Mingyu Liang
BACKGROUND: miR-29 is a master regulator of extracellular matrix genes, but conflicting data on its anti-fibrotic effect have been reported. miR-29 improves nitric oxide (NO) production in arterioles by targeting Lypla1. Mir29b1 targeted mutation exacerbates hypertension in a model derived from the Dahl salt-sensitive rat. We examined the effect of Mir29b1 mutation on tissue fibrosis and NO levels with a focus on kidney regions. METHODS: Mir29b1 targeted mutant rats on the genetic background of SS-Chr13BN rats were studied...
August 14, 2018: EBioMedicine
Xiuhua Xu, J Julie Kim, Yinuo Li, Jia Xie, Changshun Shao, Jian-Jun Wei
Uterine leiomyomas (ULM) grow under high oxidative stress due to a hypoxic microenvironment and defects in redox metabolism. AKT is one major pathway activated by reactive oxygen species (ROS) that maintains ULM growth and survival. We previously reported that AKT inactivated by AKT inhibitors can significantly induce cellular senescence in ULM cells. Since some miRNAs are induced by AKT inhibitors in an ROS-dependent manner, we proposed that these miRNAs may modulate AKT function and cellular senescence in ULM...
October 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
Tadafumi Fukata, Tsunekazu Mizushima, Junichi Nishimura, Daisuke Okuzaki, Xin Wu, Haruka Hirose, Yuhki Yokoyama, Yui Kubota, Kazuya Nagata, Naoto Tsujimura, Akira Inoue, Norikatsu Miyoshi, Naotsugu Haraguchi, Hidekazu Takahashi, Taishi Hata, Chu Matsuda, Hisako Kayama, Kiyoshi Takeda, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto
The incidence of inflammatory bowel disease (IBD) is increasing. Nucleic acid-based medicine has potential as a next-generation treatment, but it is rarely successful with IBD. The aim of this study was to establish a microRNA-based therapy in an IBD model. For this purpose, we used microRNA-29 (miR-29) and a supercarbonate apatite (sCA) nanoparticle as a drug delivery system. Injection of sCA-miR-29a-3p or sCA-miR-29b-3p into mouse tail veins markedly prevented and restored inflammation because of dextran sulfate sodium (DSS)-induced colitis...
September 7, 2018: Molecular Therapy. Nucleic Acids
Ximeng Lian, Xiaowei Chen, Jingping Sun, Guoliang An, Xiaoli Li, Yan Wang, Piye Niu, Zhonghui Zhu, Lin Tian
Silicosis is pathologically characterized by diffused pulmonary fibrosis and abundant deposition of extracellular matrix (ECM) components. The ECM is mainly secreted by myofibroblasts which are the activated state of fibroblasts. MicroRNA-29b (miR-29b) is one of the well-known microRNAs involved in fibrosis, but its roles in silicosis have not been specified. In this study, we hypothesized that miR-29b might play a protective role in the progression of silicosis. MTT assay, qRT-PCR, immunofluorescence and western blotting were applied...
November 1, 2017: Toxicology Research
Timothy Recaldin, Philip S Hobson, Elizabeth H Mann, Faruk Ramadani, David J Cousins, Paul Lavender, David J Fear
Class-switch recombination (CSR) is an essential B cell process that alters the isotype of antibody produced by the B cell, tailoring the immune response to the nature of the invading pathogen. CSR requires the activity of the mutagenic enzyme AID (encoded by AICDA) to generate chromosomal lesions within the immunoglobulin genes that initiate the class switching recombination event. These AID-mediated mutations also participate in somatic-hypermutation of the immunoglobulin variable region, driving affinity maturation...
September 2018: Molecular Immunology
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