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Retina, transplantation

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https://www.readbyqxmd.com/read/29153988/isolation-and-comparative-transcriptome-analysis-of-human-fetal-and-ipsc-derived-cone-photoreceptor-cells
#1
Emily Welby, Jorn Lakowski, Valentina Di Foggia, Dimitri Budinger, Anai Gonzalez-Cordero, Aaron T L Lun, Michael Epstein, Aara Patel, Elisa Cuevas, Kamil Kruczek, Arifa Naeem, Federico Minneci, Mike Hubank, David T Jones, John C Marioni, Robin R Ali, Jane C Sowden
Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29152454/recent-advances-in-retinal-stem-cell-therapy
#2
Sujoy Bhattacharya, Rajashekhar Gangaraju, Edward Chaum
Purpose of review: Progress in stem cell research for blinding diseases over the past decade is now being applied to patients with retinal degenerative diseases and soon perhaps, glaucoma. However, the field still has much to learn about the conversion of stem cells into various retinal cell types, and the potential delivery methods that will be required to optimize the clinical efficacy of stem cells delivered into the eye. Recent findings: Recent groundbreaking human clinical trials have demonstrated both the opportunities and current limitations of stem cell transplantation for retinal diseases...
September 2017: Current Molecular Biology Reports
https://www.readbyqxmd.com/read/29130158/crispr-in-the-retina-evaluation-of-future-potential
#3
Galaxy Y Cho, Sally Justus, Jesse D Sengillo, Stephen H Tsang
Clustered regularly interspaced short palindromic repeats (CRISPR) has been gaining widespread attention for its ability for targeted genome surgery. In treating inherited retinal degenerations, gene therapies have had varied results; the ones effective in restoring eye sight are limited by transiency in its effect. Genome surgery, however, is a solution that could potentially provide the eye with permanent healthy cells. As retinal degenerations are irreversible and the retina has little regenerative potential, permanent healthy cells are vital for vision...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29103970/detection-of-retinal-pigment-epithelium-specific-antibody-in-ipsc-derived-retinal-pigment-epithelium-transplantation-models
#4
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi
Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29098663/antibiotics-reduce-retinal-cell-survival-in-vitro
#5
Amy E Lindsey, Ellen Townes-Anderson
Antibiotics such as gentamicin (an aminoglycoside) and penicillin (a beta-lactam antibiotic) are routinely used in retinal cell and explant cultures. In many cases, these in vitro systems are testing parameters regarding photoreceptor transplantation or preparing cells for transplantation. In vivo, milligram doses of gentamicin are neurotoxic to the retina. However, little is known about the effects of antibiotics to retina in vitro and whether smaller doses of gentamicin are toxic to retinal cells. To test toxicity, retinal cells were dissociated from tiger salamander, placed in culture, and treated with either 20 μg/ml gentamicin, 100 μg/ml streptomycin, 100 U/ml antibiotic/antimycotic, 0...
November 2, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/29063192/spectrum-of-retinal-abnormalities-in-renal-transplant-patients-using-chronic-low-dose-steroids
#6
Elon H C van Dijk, Darius Soonawala, Vera Rooth, Carel B Hoyng, Onno C Meijer, Aiko P J de Vries, Camiel J F Boon
PURPOSE: To assess the ophthalmological characteristics of asymptomatic patients with a renal transplant on chronic low-dose steroids for at least the last 2 years prior to examination. METHODS: Cross-sectional study. All patients underwent an extensive ophthalmological examination. RESULTS: Of the 37 included patients [25 male, 12 female; 59 ± 11 years (range, 38-77 years)] ophthalmological phenotyping revealed abnormalities in 22 patients (59%)...
October 23, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29050186/-influent-factors-for-treating-procedure-of-cytomegalovirus-retinitis-after-allogeneic-bone-marrow-hematopoietic-stem-cell-transplantation
#7
H Miao, J Hou
Objective: To explore clinical and laboratory factors that influencing treating procedure of cytomegalovirus retinitis (CMVR) after allogeneic bone marrow hematopoietic stem cell transplantation (HSCT). Methods: This is a retrospective case series study. A total of 9 CMVR patients (15 eyes) between January 2016 and March 2017 were included in this study. All patients received intravenous or oral ganciclovir, together with intravitreous injection of ganciclovir alone or combined with foscanet sodium. One day before the first injection, aqueous humor samples from the affected eyes were collected, and CMV-DNA and interleukin-8 (IL-8) level were examined...
October 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29047024/generation-of-photoreceptor-precursors-from-mouse-embryonic-stem-cells
#8
Amy Q Lu, Colin J Barnstable
Embryonic stem cell (ESC) differentiation can be used to model development and to produce transplantable cells of the desired phenotype. ESCs can reproducibly generate retinal cells but the derivation of photoreceptor precursors is variable and depends on an array of exogenous factors and intrinsic cell-cell interactions. In this work, we have defined the use of exogenous signaling factors, dissociation, and adherent versus 3-dimensional (3D) conditions on the derivation of retinal cells from pluripotent mouse ESCs...
October 18, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29025379/survival-and-migration-of-adipose-derived-stem-cells-transplanted-in-the-injured-retina
#9
Hamid Aboutaleb Kadkhodaeian, Taki Tiraihi, Hamid Ahmadieh, Hossein Ziaei Ardakani, Narsis Daftarian, Taher Taheri
OBJECTIVES: Transplantation of stem cells is one of the approaches to treat retinal diseases. Our objective was to determine whether adipose-derived stem cell transplant can survive and migrate in the injured retina using a sodium iodate model for the pigmented retinal epithelium injury. MATERIALS AND METHODS: The adipose-derived stem cells were isolated from male albino Sprague-Dawley rats and labeled with DiI so as to track the transplants in the subretinal space...
October 12, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28979193/intraocular-injection-of-es-cell-derived-neural-progenitors-improve-visual-function-in-retinal-ganglion-cell-depleted-mouse-models
#10
Mundackal S Divya, Vazhanthodi A Rasheed, Tiffany Schmidt, Soundararajan Lalitha, Samer Hattar, Jackson James
Retinal ganglion cell (RGC) transplantation is a promising strategy to restore visual function resulting from irreversible RGC degeneration occurring in glaucoma or inherited optic neuropathies. We previously demonstrated FGF2 induced differentiation of mouse embryonic stem cells (ESC) to RGC lineage, capable of retinal ganglion cell layer (GCL) integration upon transplantation. Here, we evaluated possible improvement of visual function by transplantation of ES cell derived neural progenitors in RGC depleted glaucoma mice models...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28962643/long-term-safety-of-human-retinal-progenitor-cell-transplantation-in-retinitis-pigmentosa-patients
#11
Yong Liu, Shao Jun Chen, Shi Ying Li, Ling Hui Qu, Xiao Hong Meng, Yi Wang, Hai Wei Xu, Zhi Qing Liang, Zheng Qin Yin
BACKGROUND: Retinitis pigmentosa is a common genetic disease that causes retinal degeneration and blindness for which there is currently no curable treatment available. Vision preservation was observed in retinitis pigmentosa animal models after retinal stem cell transplantation. However, long-term safety studies and visual assessment have not been thoroughly tested in retinitis pigmentosa patients. METHODS: In our pre-clinical study, purified human fetal-derived retinal progenitor cells (RPCs) were transplanted into the diseased retina of Royal College of Surgeons (RCS) rats, a model of retinal degeneration...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28930148/elongation-of-axon-extension-for-human-ipsc-derived-retinal-ganglion-cells-by-a-nano-imprinted-scaffold
#12
Tien-Chun Yang, Jen-Hua Chuang, Waradee Buddhakosai, Wen-Ju Wu, Chen-Ju Lee, Wun-Syuan Chen, Yi-Ping Yang, Ming-Chia Li, Chi-Hsien Peng, Shih-Jen Chen
Optic neuropathies, such as glaucoma and Leber's hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs...
September 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28928775/assessment-of-safety-and-functional-efficacy-of-stem-cell-based-therapeutic-approaches-using-retinal-degenerative-animal-models
#13
REVIEW
Tai-Chi Lin, Magdalene J Seiler, Danhong Zhu, Paulo Falabella, David R Hinton, Dennis O Clegg, Mark S Humayun, Biju B Thomas
Dysfunction and death of retinal pigment epithelium (RPE) and or photoreceptors can lead to irreversible vision loss. The eye represents an ideal microenvironment for stem cell-based therapy. It is considered an "immune privileged" site, and the number of cells needed for therapy is relatively low for the area of focused vision (macula). Further, surgical placement of stem cell-derived grafts (RPE, retinal progenitors, and photoreceptor precursors) into the vitreous cavity or subretinal space has been well established...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28844659/recapitulation-of-human-retinal-development-from-human-pluripotent-stem-cells-generates-transplantable-populations-of-cone-photoreceptors
#14
Anai Gonzalez-Cordero, Kamil Kruczek, Arifa Naeem, Milan Fernando, Magdalena Kloc, Joana Ribeiro, Debbie Goh, Yanai Duran, Samuel J I Blackford, Laura Abelleira-Hervas, Robert D Sampson, Ian O Shum, Matthew J Branch, Peter J Gardner, Jane C Sowden, James W B Bainbridge, Alexander J Smith, Emma L West, Rachael A Pearson, Robin R Ali
Transplantation of rod photoreceptors, derived either from neonatal retinae or pluripotent stem cells (PSCs), can restore rod-mediated visual function in murine models of inherited blindness. However, humans depend more upon cone photoreceptors that are required for daylight, color, and high-acuity vision. Indeed, macular retinopathies involving loss of cones are leading causes of blindness. An essential step for developing stem cell-based therapies for maculopathies is the ability to generate transplantable human cones from renewable sources...
September 12, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28829422/development-of-a-refined-protocol-for-trans-scleral-subretinal-transplantation-of-human-retinal-pigment-epithelial-cells-into-rat-eyes
#15
Cuiping Zhao, Nathan C Boles, Justine D Miller, Suzanne Kawola, Sally Temple, Richard J Davis, Jeffrey H Stern
Degenerative retinal diseases such as age-related macular degeneration (AMD) are the leading cause of irreversible vision loss worldwide. AMD is characterized by the degeneration of retinal pigment epithelial (RPE) cells, which are a monolayer of cells functionally supporting and anatomically wrapping around the neural retina. Current pharmacological treatments for the non-neovascular AMD (dry AMD) only slow down the disease progression but cannot restore vision, necessitating studies aimed at identifying novel therapeutic strategies...
August 12, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28827082/mfsd2a-promotes-endothelial-generation-of-inflammation-resolving-lipid-mediators-and-reduces-colitis%C3%A2-in%C3%A2-mice
#16
Federica Ungaro, Carlotta Tacconi, Luca Massimino, Paola Antonia Corsetto, Carmen Correale, Philippe Fonteyne, Andrea Piontini, Valeria Garzarelli, Francesca Calcaterra, Silvia Della Bella, Antonino Spinelli, Michele Carvello, Angela Maria Rizzo, Stefania Vetrano, Luciana Petti, Gionata Fiorino, Federica Furfaro, Domenico Mavilio, Krishna Rao Maddipati, Alberto Malesci, Laurent Peyrin-Biroulet, Silvia D'Alessio, Silvio Danese
BACKGROUND & AIMS: Alterations in signaling pathways that regulate resolution of inflammation (resolving pathways) contribute to pathogenesis of ulcerative colitis (UC). The resolution process is regulated by lipid mediators, such as those derived from the ω-3 docosahexaenoic acid (DHA), whose esterified form is transported by the major facilitator superfamily domain containing 2A (MFSD2A) through the endothelium of brain, retina, and placenta. We investigated if and how MFSD2A regulates lipid metabolism of gut endothelial cells to promote resolution of intestinal inflammation...
August 4, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28794849/scaffolds-for-retinal-pigment-epithelial-cell-transplantation-in-age-related-macular-degeneration
#17
REVIEW
Corina E White, Ronke M Olabisi
In several retinal degenerative diseases, including age-related macular degeneration, the retinal pigment epithelium, a highly functionalized cell monolayer, becomes dysfunctional. These retinal diseases are marked by early retinal pigment epithelium dysfunction reducing its ability to maintain a healthy retina, hence making the retinal pigment epithelium an attractive target for treatment. Cell therapies, including bolus cell injections, have been investigated with mixed results. Since bolus cell injection does not promote the proper monolayer architecture, scaffolds seeded with retinal pigment epithelium cells and then implanted have been increasingly investigated...
January 2017: Journal of Tissue Engineering
https://www.readbyqxmd.com/read/28715614/intraperitoneal-administration-of-adipose-tissue-derived-stem-cells-for-the-rescue-of-retinal-degeneration-in-a-mouse-model-via-indigenous-cntf-up-regulation-by-il-6
#18
Jeong Hoon Heo, Jung Ae Yoon, Eun Kyung Ahn, Hyun Kim, Sang Hwa Urm, Chul Oh Oak, Byeng Chul Yu, Sang Joon Lee
As the world's population begins to age, retinal degeneration is an increasing problem, and various treatment modalities are being developed. However, there have been no therapies for degenerative retinal conditions that are not characterized by neovascularization. We investigated whether transplantation of mouse adipose tissue-derived stem cells (mADSC) into the intraperitoneal space has a rescue effect on NaIO3 -induced retinal degeneration in mice. In this study, mADSC transplantation recovered visual function and preserved the retinal outer layer structure compared to the control group without any integration of mADSC into the retina...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28695744/genome-editing-the-breakthrough-technology-for-inherited-retinal-disease
#19
Andrew J Smith, Stephen P Carter, Breandán N Kennedy
Genetic alterations resulting in a dysfunctional retinal pigment epithelium and/or degenerating photoreceptors cause impaired vision. These juxtaposed cells in the retina of the posterior eye are crucial for the visual cycle or phototransduction. Deficits in these biochemical processes perturb neural processing of images capturing the external environment. Notably, there is a distinct lack of clinically approved pharmacological, cell- or gene-based therapies for inherited retinal disease. Gene editing technologies are rapidly advancing as a realistic therapeutic option...
July 11, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28691584/fibrin-gel-as-a-scaffold-for-photoreceptor-cells-differentiation-from-conjunctiva-mesenchymal-stem-cells-in-retina-tissue-engineering
#20
Mostafa Soleimannejad, Somayeh Ebrahimi-Barough, Masoud Soleimani, Samad Nadri, Seyed Mohammad Tavangar, Ramak Roohipoor, Meysam Yazdankhah, Neda Bayat, Mohammad Riazi-Esfahani, Jafar Ai
Stem cell-based therapies are attraction approaches for regenerative medicine for treating retinal diseases. One of the limitations in cell therapy is cell death following post-injection whit preventing functional integration with retinal tissue. Fibrin gel, a bio-polymeric material with excellent biocompatibility, provides numerous advantages as a tissue engineering scaffold and a stem cell carrier. Therefore, current research is focusing on developing fibrin hydrogel scaffolds to protect stem cells during delivery and to stimulate endogenous regeneration through interactions of transplanted stem cells and retinal tissue...
July 10, 2017: Artificial Cells, Nanomedicine, and Biotechnology
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