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Retina, transplantation

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https://www.readbyqxmd.com/read/28322827/structural-divergence-of-essential-triad-ribbon-synapse-proteins-among-placental-mammals-implications-for-preclinical-trials-in-photoreceptor-transplantation-therapy
#1
Christopher R J Laver, Joanne A Matsubara
As photoreceptor transplantation rapidly moves closer to the clinic, verifying graft efficacy in animal models may have unforeseen xenogeneic barriers. Although photoreceptor transplants have most convincingly exhibited functional synaptogenesis in conspecific studies, such evidence (while ruling out false-positives due to: viral graft labeling, fusion/cytosolic transfer, or neuroprotection) has not yet been shown for discordant xenografts. From this, a fundamental question should be raised: is useful xenosynaptogenesis likely between human photoreceptors and mouse retina? The triad ribbon synapse (TRS) that would normally form is unique and contains trans-synaptic proteins essential to its formation and function...
March 18, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28321468/hyperglycaemic-memory-affects-the-neurovascular-unit-of-the-retina-in-a-diabetic-mouse-model
#2
Patrick Friedrichs, Andrea Schlotterer, Carsten Sticht, Matthias Kolibabka, Paulus Wohlfart, Axel Dietrich, Thomas Linn, Grietje Molema, Hans-Peter Hammes
AIMS/HYPOTHESIS: The aim of this study was to evaluate damage to the neurovascular unit in a mouse model of hyperglycaemic memory. METHODS: A streptozotocin-induced mouse model of diabetes (C57BL/6J background) received insulin-releasing pellets and pancreatic islet-cell transplantation. Damage to the neurovascular unit was studied by quantitative retinal morphometry for microvascular changes and microarray analysis, with subsequent functional annotation clustering, for changes of the retinal genome...
March 20, 2017: Diabetologia
https://www.readbyqxmd.com/read/28298640/combined-transplantation-of-human-mesenchymal-stem-cells-and-human-retinal-progenitor-cells-into-the-subretinal-space-of-rcs-rats
#3
Linghui Qu, Lixiong Gao, Haiwei Xu, Ping Duan, Yuxiao Zeng, Yong Liu, Zheng Qin Yin
Retinitis pigmentosa (RP) is one of hereditary retinal diseases characterized by the loss of photoreceptors. Cell transplantation has been clinically applied to treat RP patients. Human retinal progenitor cells (HRPCs) and human bone marrow-derived mesenchymal stem cells (HBMSCs) are the two commonly and practically used stem cells for transplantation. Since combined transplantation could be a promising way to integrate the advantages of both stem cell types, we transplanted HRPCs and HBMSCs into the subretinal space (SRS) of Royal College of Surgeons (RCS) rats...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28296613/autologous-induced-stem-cell-derived-retinal-cells-for-macular-degeneration
#4
Michiko Mandai, Akira Watanabe, Yasuo Kurimoto, Yasuhiko Hirami, Chikako Morinaga, Takashi Daimon, Masashi Fujihara, Hiroshi Akimaru, Noriko Sakai, Yumiko Shibata, Motoki Terada, Yui Nomiya, Shigeki Tanishima, Masahiro Nakamura, Hiroyuki Kamao, Sunao Sugita, Akishi Onishi, Tomoko Ito, Kanako Fujita, Shin Kawamata, Masahiro J Go, Chikara Shinohara, Ken-Ichiro Hata, Masanori Sawada, Midori Yamamoto, Sachiko Ohta, Yasuo Ohara, Kenichi Yoshida, Junko Kuwahara, Yuko Kitano, Naoki Amano, Masafumi Umekage, Fumiyo Kitaoka, Azusa Tanaka, Chihiro Okada, Naoko Takasu, Seishi Ogawa, Shinya Yamanaka, Masayo Takahashi
We assessed the feasibility of transplanting a sheet of retinal pigment epithelial (RPE) cells differentiated from induced pluripotent stem cells (iPSCs) in a patient with neovascular age-related macular degeneration. The iPSCs were generated from skin fibroblasts obtained from two patients with advanced neovascular age-related macular degeneration and were differentiated into RPE cells. The RPE cells and the iPSCs from which they were derived were subject to extensive testing. A surgery that included the removal of the neovascular membrane and transplantation of the autologous iPSC-derived RPE cell sheet under the retina was performed in one of the patients...
March 16, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28286571/we-are-not-alone-a-case-for-the-human-microbiome-in-extra-intestinal-diseases
#5
EDITORIAL
S Shivaji
BACKGROUND: "Dysbiosis" in the gut microbiome has been implicated in auto-immune diseases, in inflammatory diseases, in some cancers and mental disorders. The challenge is to unravel the cellular and molecular basis of dysbiosis so as to understand the disease manifestation. MAIN BODY: Next generation sequencing and genome enabled technologies have led to the establishment of the composition of gut microbiomes and established that "dysbiosis" is the cause of several diseases...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28258056/novel-immunodeficient-pde6b-rd1-mouse-model-of-retinitis-pigmentosa-to-investigate-potential-therapeutics-and-pathogenesis-of-retinal-degeneration
#6
Alaknanda Mishra, Barun Das, Madhu Nath, Srikanth Iyer, Ashwani Kesarwani, Jashdeep Bhattacharjee, Shailendra Arindkar, Preeti Sahay, Kshama Jain, Parul Sahu, Prakriti Sinha, Thirumurthy Velpandian, Perumal Nagarajan, Pramod Upadhyay
Retinitis pigmentosa (RP) is a common retinal degeneration disease caused by mutation in any gene of the photo transduction cascade and results in photoreceptor dystrophy. Over decades, several animal models have been used to address the need for elucidation of effective therapeutics and factors regulating retinal degeneration to prohibit or renew the damaged retina. However, controversies over immune privilege of retina during cell transplantation and role of immune modulation during RP still remain largely uninvestigated due to lack of proper animal models...
March 3, 2017: Biology Open
https://www.readbyqxmd.com/read/28223209/langmuir-schaefer-film-deposition-onto-honeycomb-porous-films-for-retinal-tissue-engineering
#7
Maria Teresa Calejo, Tanja Ilmarinen, Elina Vuorimaa-Laukkanen, Elina Talvitie, Hanna M Hakola, Heli Skottman, Minna Kellomäki
Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye - the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch's membrane as carriers for RPE cells...
February 18, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28220575/generation-of-storable-retinal-organoids-and-retinal-pigmented-epithelium-from-adherent-human-ips-cells-in-xeno-free-and-feeder-free-conditions
#8
Sacha Reichman, Amélie Slembrouck, Giuliana Gagliardi, Antoine Chaffiol, Angélique Terray, Céline Nanteau, Anais Potey, Morgane Belle, Oriane Rabesandratana, Jens Duebel, Gael Orieux, Emeline F Nandrot, José-Alain Sahel, Olivier Goureau
Human induced pluripotent stem cells (hiPSCs) are potentially useful in regenerative therapies for retinal disease. For medical applications, therapeutic retinal cells, such as retinal pigmented epithelial (RPE) cells or photoreceptor precursors, must be generated under completely defined conditions. To this purpose, we have developed a two-step xeno-free/feeder-free (XF/FF) culture system to efficiently differentiate hiPSCs into retinal cells. This simple method, relies only on adherent hiPSCs cultured in chemically defined media, bypassing embryoid body formation...
February 20, 2017: Stem Cells
https://www.readbyqxmd.com/read/28216299/hipsc-derived-retinal-ganglion-cells-grow-dendritic-arbors-and-functional-axons-on-a-tissue-engineered-scaffold
#9
Kangjun Li, Xiufeng Zhong, Sijing Yang, Ziming Luo, Kang Li, Ying Liu, Song Cai, Huaiyu Gu, Shoutao Lu, Haijun Zhang, Yantao Wei, Jing Zhuang, Yehong Zhuo, Zhigang Fan, Jian Ge
Numerous therapeutic procedures in modern medical research rely on the use of tissue engineering for the treatment of retinal diseases. However, the cell source and the transplantation method are still a limitation. Previously, we reported on the induction of a self-organizing three-dimensional neural retina from human-induced pluripotent stem cells (hiPSCs). In this study, we disclose the generation of retinal ganglion cells (RGCs) from the neural retina and their seeding on a biodegradable poly (lactic-co-glycolic acid) (PLGA) scaffold to create an engineered RGC-scaffold biomaterial...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28210901/transplantation-of-lineage-negative-stem-cells-in-pterygopalatine-artery-ligation-induced-retinal-ischemia-reperfusion-injury-in-mice
#10
Gillipsie Minhas, Sudesh Prabhakar, Ryuichi Morishita, Munehisa Shimamura, Reema Bansal, Akshay Anand
Retinal ischemia is a condition associated with retinal degenerative diseases such as glaucoma, diabetic retinopathy, and other optic neuropathies, leading to visual impairment and blindness worldwide. Currently, there is no therapy available for ischemic retinopathies. Therefore, the aim of this study was to test a murine model of pterygopalatine artery ligation-induced retinal injury for transplantation of mouse bone marrow-derived lineage-negative (lin-ve) stem cells. The mouse external carotid artery and pterygopalatine artery were ligated for 3...
February 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28205557/experimental-study-of-the-biological-properties-of-human-embryonic-stem-cell-derived-retinal-progenitor-cells
#11
Jingzhi Shao, Peng-Yi Zhou, Guang-Hua Peng
Retinal degenerative diseases are among the leading causes of blindness worldwide, and cell replacement is considered as a promising therapeutic. However, the resources of seed cells are scarce. To further explore this type of therapy, we adopted a culture system that could harvest a substantial quantity of retinal progenitor cells (RPCs) from human embryonic stem cells (hESCs) within a relatively short period of time. Furthermore, we transplanted these RPCs into the subretinal spaces of Royal College of Surgeons (RCS) rats...
February 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28199830/ipsc-derived-retina-transplants-improve-vision-in-rd1-end-stage-retinal-degeneration-mice
#12
Michiko Mandai, Momo Fujii, Tomoyo Hashiguchi, Genshiro A Sunagawa, Shin-Ichiro Ito, Jianan Sun, Jun Kaneko, Junki Sho, Chikako Yamada, Masayo Takahashi
No abstract text is available yet for this article.
February 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28185609/olfactory-ensheathing-cells-inhibit-gliosis-in-retinal-degeneration-by-down-regulation-of-the-m%C3%A3-ller-cell-notch-signaling-pathway
#13
Jing Xie, Shujia Huo, Yijian Li, Jiaman Dai, Haiwei Xu, Zheng Qin Yin
Retinal regeneration and self-repair, whether in response to injury or degenerative disease, are severely impeded by glial scar formation by Müller cells (specialized retinal macroglia). We have previously demonstrated that the activation of Müller cells and gliosis in the degenerative retina are significantly suppressed by the subretinal transplantation of a mixture of olfactory ensheathing cells (OECs) and olfactory nerve fibroblasts. However, the underlying molecular mechanism has remained elusive. Here, we transplanted purified rat OECs into the subretinal space of pigmented Royal College of Surgeons (RCS) rats, a classic rodent model of retinal degeneration...
February 9, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28183612/controlled-surface-morphology-and-hydrophilicity-of-polycaprolactone-toward-human-retinal-pigment-epithelium-cells
#14
Saleheh Shahmoradi, Fatemeh Yazdian, Fatemeh Tabandeh, Zahra-Soheila Soheili, Ashraf Sadat Hatamian Zarami, Mona Navaei-Nigjeh
Applying scaffolds as a bed to enhance cell proliferation and even differentiation is one of the treatment of retina diseases such as age-related macular degeneration (AMD) which deteriorating photoreceptors and finally happening blindness. In this study, aligned polycaprolactone (PCL) nanofibers were electrospun and at different conditions and their characteristics were measured by scanning electron microscope (SEM) and contact angle. Response surface methodology (RSM) was used to optimize the diameter of fabricated nanofibers...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28164440/effect-of-human-umbilical-cord-blood-mesenchymal-stem-cells-administered-by-intravenous-or-intravitreal-routes-on-cryo-induced-retinal-injury
#15
Eman M Mohamed, Shaimaa A Abdelrahman, Samia Hussein, Sally M Shalaby, Hala Mosaad, Ahmed M B Awad
Traumatic optic neuropathy is an important cause of severe vision loss. So, many attempts were performed to transplant stem cells systemically or locally to regenerate the injured retina. In this study, we investigated the effect of human umbilical cord blood mesenchymal stem cells (hUBMSCs) on histological structure, apoptotic, antiapoptotic, oxidant and antioxidant markers in an experimental model of cryo-induced retinal damage in mice. Forty-eight mice were included with 4 major groups; group I contained 18 mice as controls...
February 5, 2017: IUBMB Life
https://www.readbyqxmd.com/read/28155808/using-electrical-stimulation-to-enhance-the-efficacy-of-cell-transplantation-therapies-for-neurodegenerative-retinal-diseases-concepts-challenges-and-future-perspectives
#16
Abby Leigh Manthey, Wei Liu, Zhi Xin Jiang, Marcus Hiu Kong Lee, Jian Ji, Kwok-Fai So, Jimmy Shiu Ming Lai, Vincent Wing Hong Lee, Kin Chiu
Disease or trauma-induced loss or dysfunction of neurons in any central nervous system (CNS) tissue will have a significant impact on the health of the affected patient. The retina is a multilayered tissue that originates from the neuroectoderm, much like the brain and spinal cord. While sight is not required for life, neurodegeneration-related loss of vision not only affects the quality of life for the patient, but also has societal implications in terms of health care expenditure. Thus, it is essential to develop effective strategies to repair the retina and prevent disease symptoms...
February 3, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28149774/neuroprotective-effects-of-bdnf-and-gdnf-in-intravitreally-transplanted-mesenchymal-stem-cells-after-optic-nerve-crush-in-mice
#17
Zong-Li Hu, Ni Li, Xin Wei, Li Tang, Ting-Hua Wang, Xiao-Ming Chen
AIM: To assess the neuro-protective effect of bone marrow mesenchymal stem cells (BMSCs) on retinal ganglion cells (RGCs) following optic nerve crush in mice. METHODS: C56BL/6J mice were treated with intravitreal injection of PBS, BMSCs, BDNF-interference BMSCs (BIM), and GDNF-interference BMSCs (GIM) following optic nerve crush, respectively. The number of surviving RGCs was determined by whole-mount retinas and frozen sections, while certain mRNA or protein was detected by q-PCR or ELISA, respectively...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28143742/cone-genesis-tracing-by-the-chrnb4-egfp-mouse-line-evidences-of-cellular-material-fusion-after-cone-precursor-transplantation
#18
Sarah Decembrini, Catherine Martin, Florian Sennlaub, Sylvain Chemtob, Martin Biel, Marijana Samardzija, Alexandre Moulin, Francine Behar-Cohen, Yvan Arsenijevic
The cone function is essential to mediate high visual acuity, color vision, and daylight vision. Inherited cone dystrophies and age-related macular degeneration affect a substantial percentage of the world population. To identify and isolate the most competent cells for transplantation and integration into the retina, cone tracing during development would be an important added value. To that aim, the Chrnb4-EGFP mouse line was characterized throughout retinogenesis. It revealed a sub-population of early retinal progenitors expressing the reporter gene that is progressively restricted to mature cones during retina development...
March 1, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28129425/vision-recovery-and-connectivity-by-fetal-retinal-sheet-transplantation-in-an-immunodeficient-retinal-degenerate-rat-model
#19
Magdalene J Seiler, Robert E Lin, Bryce T McLelland, Anuradha Mathur, Bin Lin, Jaclyn Sigman, Alexander T De Guzman, Leonard M Kitzes, Robert B Aramant, Biju B Thomas
Purpose: To characterize a recently developed model, the retinal degenerate immunodeficient S334ter line-3 rat (SD-Foxn1 Tg(S334ter)3Lav) (RD nude rat), and to test whether transplanted rat fetal retinal sheets can elicit lost responses to light. Methods: National Institutes of Health nude rats (SD-Foxn1 Tg) with normal retina were compared to RD nude rats with and without transplant for morphology and visual function. Retinal sheets from transgenic rats expressing human placental alkaline phosphatase (hPAP) were transplanted into the subretinal space of RD nude rats between postnatal day (P) 26 and P38...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28099524/role-of-myo-nog-cells-in-neuroprotection-evidence-from-the-light-damaged-retina
#20
Alice Brandli, Jacquelyn Gerhart, Christopher K Sutera, Sivaraman Purushothuman, Mindy George-Weinstein, Jonathan Stone, Arturo Bravo-Nuevo
PURPOSE: To identify Myo/Nog cells in the adult retina and test their role in protecting retinal photoreceptors from light damage. METHODS: Light damage was induced by exposing albino rats raised in dim cyclic light to 1000 lux light for 24 hours. In one group of rats, Myo/Nog cells were purified from rat brain tissue by magnetic cell sorting following binding of the G8 monoclonal antibody (mAb). These cells were injected into the vitreous humour of the eye within 2 hours following bright light exposure...
2017: PloS One
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