keyword
https://read.qxmd.com/read/37739336/loss-of-dicer-in-newborn-melanocytes-leads-to-premature-hair-greying-and-changes-in-integrin-expression
#1
JOURNAL ARTICLE
Juliette U Bertrand, Valérie Petit, Zackie Aktary, Pierre DE LA Grange, Nadav Elkoshi, Pierre Sohier, Véronique Delmas, Carmit Levy, Lionel Larue
Premature hair greying occurs due to the depletion of melanocyte stem cells (McSCs) in the hair follicle, which can be accelerated by stress caused by genetic or environmental factors. However, the connection between stress and McSC loss is not fully understood. MiRNAs are molecules that control gene expression by regulating mRNA stability and translation, and are produced by the enzyme Dicer, which is repressed under stress. Here, using two mouse genetic models and human and mouse cell lines, we found that the inactivation of Dicer in melanocytes leads to a misplacement of these cells within the hair follicle, resulting in a lack of melanin transfer to keratinocytes in the growing hair and the exhaustion of the McSC pool...
September 20, 2023: Journal of Investigative Dermatology
https://read.qxmd.com/read/37255601/developmentally-regulated-expression-of-integrin-alpha-6-distinguishes-neural-crest-derivatives-in-the-skin
#2
JOURNAL ARTICLE
Shize Ma, Xiu Li, Rui Cao, Guoqin Zhan, Xin Fu, Ran Xiao, Zhigang Yang
Neural crest-derived cells play essential roles in skin function and homeostasis. However, how they interact with environmental cues and differentiate into functional skin cells remains unclear. Using a combination of single-cell data analysis, neural crest lineage tracing, and flow cytometry, we found that the expression of integrin α6 (ITGA6) in neural crest and its derivatives was developmentally regulated and that ITGA6 could serve as a functional surface marker for distinguishing neural crest derivatives in the skin...
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37209167/differential-expression-of-extracellular-matrix-proteins-in-the-lesional-skin-of-vitiligo-patients
#3
JOURNAL ARTICLE
Seema Rani, Naveed Pervaiz, Davinder Parsad, Ravinder Kumar
Skin pigmentation is regulated by intricate interaction of the dermis and epidermis. The extracellular components present in the dermis play a very important role in the maintenance of skin homeostasis. Therefore, our objective was to check the expression of various ECM components secreted by the dermal fibroblasts in the lesional skin and non-lesional skin of vitiligo patients. For this study, skin punch biopsies (4 mm) were collected from lesional skin (n = 12), non-lesional skin (n = 6) of non-segmental vitiligo patient's (NSV) and healthy control skin (n = 10)...
May 20, 2023: Archives of Dermatological Research
https://read.qxmd.com/read/37108705/characterization-of-porcine-ocular-surface-epithelial-microenvironment
#4
JOURNAL ARTICLE
Naresh Polisetti, Gottfried Martin, Heidi R Cristina Schmitz, Ursula Schlötzer-Schrehardt, Günther Schlunck, Thomas Reinhard
The porcine ocular surface is used as a model of the human ocular surface; however, a detailed characterization of the porcine ocular surface has not been documented. This is due, in part, to the scarcity of antibodies produced specifically against the porcine ocular surface cell types or structures. We performed a histological and immunohistochemical investigation on frozen and formalin-fixed, paraffin-embedded ocular surface tissue from domestic pigs using a panel of 41 different antibodies related to epithelial progenitor/differentiation phenotypes, extracellular matrix and associated molecules, and various niche cell types...
April 19, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36653710/analysis-of-integrin-dependent-melanoblast-migration-during-development
#5
JOURNAL ARTICLE
Amanda Haage, Guy Tanentzapf
The neural crest is a transient embryonic structure that gives rise to a number of important cell types and tissues, including most of the peripheral and enteric nervous systems, pigment-producing skin cells known as melanocytes, and many craniofacial structures. Melanoblasts, the precursors of melanocytes, are derived from the so-called trunk neural crest cells. These cells delaminate and migrate along a dorsolateral pathway to colonize their final destination in the skin, and consequently, defects in melanoblast migration result in pigmentation defects...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/35727432/glycan-epitope-and-integrin-expression-dynamics-characterize-neural-crest-epithelial-to-mesenchymal-transition-emt-in-human-pluripotent-stem-cell-differentiation
#6
JOURNAL ARTICLE
Ria Thomas, Vishal Menon, Rakesh Mani, Jan Pruszak
The neural crest gives rise to progeny as diverse as peripheral neurons, myelinating cells, cranial muscle, bone and cartilage tissues, and melanocytes. Neural crest derivation encompasses complex morphological change, including epithelial-to-mesenchymal transition (EMT) and migration to the eventual target locations throughout the body. Neural crest cultures derived from stem cells provide an attractive source for developmental studies in human model systems, of immediate biomedical relevance for neurocristopathies, neural cancer biology and regenerative medicine, if only appropriate markers for lineage and cell type definition and quality control criteria were available...
June 21, 2022: Stem cell reviews and reports
https://read.qxmd.com/read/34439879/cell-adhesion-molecules-in-normal-skin-and-melanoma
#7
REVIEW
Cian D'Arcy, Christina Kiel
Cell adhesion molecules (CAMs) of the cadherin, integrin, immunoglobulin, and selectin protein families are indispensable for the formation and maintenance of multicellular tissues, especially epithelia. In the epidermis, they are involved in cell-cell contacts and in cellular interactions with the extracellular matrix (ECM), thereby contributing to the structural integrity and barrier formation of the skin. Bulk and single cell RNA sequencing data show that >170 CAMs are expressed in the healthy human skin, with high expression levels in melanocytes, keratinocytes, endothelial, and smooth muscle cells...
August 15, 2021: Biomolecules
https://read.qxmd.com/read/34389315/characterisation-of-corneas-following-different-time-and-storage-methods-for-their-use-as-a-source-of-stem-like-limbal-epithelial-cells
#8
JOURNAL ARTICLE
Cristina Romo-Valera, Miguel Pérez-Garrastachu, Raquel Hernáez-Moya, Maddalen Rodriguez-Astigarraga, Paula Romano-Ruiz, Jaime Etxebarria, Jon Arluzea, Noelia Andollo
The transplantation of expansions of limbal epithelial stem cells (LESC) remains one of the most efficient therapies for the treatment of limbal stem cell deficiency (LSCD) to date. However, the available donor corneas are scarce, and the corneas conserved for long time, under hypothermic conditions (after 7 days) or in culture (more than 28 days), are usually discarded due to poor viability of the endothelial cells. To establish an objective criterion for the utilisation or discarding of corneas as a source of LESC, we characterized, by immunohistochemistry analysis, donor corneas conserved in different conditions and for different periods of time...
August 11, 2021: Experimental Eye Research
https://read.qxmd.com/read/33846376/low-expression-of-the-ppar%C3%AE-regulated-gene-thioredoxin-interacting-protein-accompanies-human-melanoma-progression-and-promotes-experimental-lung-metastases
#9
JOURNAL ARTICLE
Patrick Meylan, Christine Pich, Carine Winkler, Stefanie Ginster, Lionel Mury, Marie Sgandurra, René Dreos, Dennie Tompers Frederick, Marc Hammond, Genevieve Marie Boland, Liliane Michalik
The thioredoxin system plays key roles in regulating cancer cell malignancy. Here we identify the Thioredoxin-interacting protein (TXNIP) as a gene, which expression is regulated by PPARγ in melanoma cells. We show that high TXNIP expression levels associate with benign melanocytic lesions, with tumor regression in patients on MAP kinase targeted therapy, with decreased proliferation in patients' melanoma biopsies, and with cell cycle arrest in human melanoma cell lines. In contrast, reduced TXNIP expression associates with advanced melanoma and with disease progression in patients...
April 12, 2021: Scientific Reports
https://read.qxmd.com/read/33050649/uveal-melanoma-derived-extracellular-vesicles-display-transforming-potential-and-carry-protein-cargo-involved-in-metastatic-niche-preparation
#10
JOURNAL ARTICLE
Thupten Tsering, Alexander Laskaris, Mohamed Abdouh, Prisca Bustamante, Sabrina Parent, Eva Jin, Sarah Tadhg Ferrier, Goffredo Arena, Julia V Burnier
Extracellular vesicles (EVs) carry molecules derived from donor cells and are able to alter the properties of recipient cells. They are important players during the genesis and progression of tumors. Uveal melanoma (UM) is the most common primary intraocular tumor in adults and is associated with a high rate of metastasis, primarily to the liver. However, the mechanisms underlying this process are poorly understood. In the present study, we analyzed the oncogenic potential of UM-derived EVs and their protein signature...
October 11, 2020: Cancers
https://read.qxmd.com/read/32606961/long-noncoding-rna-ccat1-functions-as-a-competing-endogenous-rna-to-upregulate-itga9-by-sponging-mir-296-3p-in-melanoma
#11
JOURNAL ARTICLE
Jinghua Fan, Xiaoxiao Kang, Limin Zhao, Yan Zheng, Jun Yang, Di Li
Background: Melanoma is aggressive and lethal melanocytic neoplasm, and its incidence has increased worldwide in recent decades. Accumulating evidence has showed that various long noncoding RNAs (lncRNAs) participated in occurrence of malignant tumors, including melanoma. The present study was designed to investigate function of lncRNA colon cancer-associated transcript-1 (CCAT1) in melanoma. Methods: The expression levels of CCAT1, miR-296-3p and Integrin alpha9 (ITGA9) in melanoma tissues or cells were measured using real-time quantitative polymerase chain reaction (RT-qPCR)...
2020: Cancer Management and Research
https://read.qxmd.com/read/32580934/precise-coordination-of-cell-ecm-adhesion-is-essential-for-efficient-melanoblast-migration-during-development
#12
JOURNAL ARTICLE
Amanda Haage, Kelsey Wagner, Wenjun Deng, Bhavya Venkatesh, Caitlin Mitchell, Katharine Goodwin, Aaron Bogutz, Louis Lefebvre, Catherine D Van Raamsdonk, Guy Tanentzapf
Melanoblasts disperse throughout the skin and populate hair follicles through long-range cell migration. During migration, cells undergo cycles of coordinated attachment and detachment from the Extra-Cellular Matrix (ECM). Embryonic migration processes that require cell-ECM attachment are dependent on the integrin family of adhesion receptors. Precise regulation of integrin-mediated adhesion is important for many developmental migration events. However, the mechanisms that regulate integrin-mediated adhesion in vivo in melanoblasts are not well understood...
June 24, 2020: Development
https://read.qxmd.com/read/32572848/screening-of-integrins-localized-on-the-surface-of-human-epidermal-melanocytes
#13
JOURNAL ARTICLE
Seong Jae Kim, Min Seong Kim, Hye Jin Park, Hyun Lee, Jung Im Yun, Hye Won Lim, Seung Tae Lee
In vivo, melanocytes occupy three-dimensional (3D) space. Nevertheless, most experiments involving melanocytes are performed in a two-dimensional microenvironment, resulting in difficulty obtaining accurate results. Therefore, it is necessary to construct an artificial in vivo-like 3D microenvironment. Here, as a step towards engineering a precisely defined acellular 3D microenvironment supporting the maintenance of human epidermal melanocytes (HEMs), we examined the types of integrin heterodimers that are expressed transcriptionally, translationally, and functionally in HEMs...
June 23, 2020: In Vitro Cellular & Developmental Biology. Animal
https://read.qxmd.com/read/32066306/quantitative-and-qualitative-analysis-of-integrin-subtype-expression-in-melanocytes-and-melanoma-cells
#14
JOURNAL ARTICLE
Verena Kopatz, Edgar Selzer
Objectives: Changes in the integrin expression pattern have been associated with the malignant transformation of melanocytes suggesting that integrins may be potential biomarkers as well as molecular targets for individualized therapy. Since there is a lack of comprehensive qualitative and quantitative expression data, we characterized the integrin expression profile in normal and malignant human cells of the melanocytic lineage. Methods: Seven melanoma cell lines as well as normal human melanocytes were investigated in western blots including recombinant integrin subunits for quantification...
February 17, 2020: Journal of Receptor and Signal Transduction Research
https://read.qxmd.com/read/32049584/integrin-linked-kinase-regulates-melanosome-trafficking-and-melanin-transfer-in-melanocytes
#15
JOURNAL ARTICLE
Melissa Crawford, Nancy Liu, Elahe Mahdipour, Kevin Barr, Bryan Heit, Lina Dagnino
Melanosomes are melanin-containing organelles that provide pigmentation and protection from solar UV radiation to the skin. In melanocytes, melanosomes mature and traffic to dendritic tips, where they are transferred to adjacent epidermal keratinocytes through pathways that involve microtubule networks and the actin cytoskeleton. However, the role of scaffold proteins in these processes is poorly understood. Integrin-linked kinase (ILK) is a scaffold protein that regulates microtubule stability and F-actin dynamics...
April 1, 2020: Molecular Biology of the Cell
https://read.qxmd.com/read/31880593/clinicopathological-analysis-of-acquired-melanocytic-nevi-and-a-preliminary-study-on-the-possible-origin-of-nevus-cells
#16
JOURNAL ARTICLE
Da-Guang Wang, Fei-Ran Huang, Wei Chen, Yuan Zhou, Cong-Yang Wang, Feng Zhu, Bing-Jie Shao, Dan Luo
BACKGROUND: The pathogenesis of acquired melanocytic nevi (AMN) is still unclear, and the origin of nevus cells has not been clarified. OBJECTIVE: To analyze the clinical features and pathological types of AMN and identify the possible origin of nevus cells. METHODS: A retrospective study of 2929 cases of AMN was conducted, and 96 specimens of intradermal and junctional nevi were selected. Immunohistochemical assays were performed to detect the expression of basement membrane component receptor DDR-1 and the molecular markers on epidermal melanocytes, dermal stem cells (DSCs), and hair follicle stem cells...
December 26, 2019: American Journal of Dermatopathology
https://read.qxmd.com/read/31499059/mir-9-regulates-melanocytes-adhesion-and-migration-during-vitiligo-repigmentation-induced-by-uvb-treatment
#17
JOURNAL ARTICLE
Mengyun Su, Hong Yi, Xiaolei He, Longfei Luo, Shan Jiang, Ying Shi
The decreased adhesion ability of melanocytes to the neighboring keratinocytes prompts melanocytes to lose from the epidermis, comprising the critical step in vitiligo pathogenesis. The repigmentation process involves the migration of melanocytes to the lesional area. This study aims to investigate the role and mechanism of microRNA (miR)-9 in the adhesion and migration of melanocytes during vitiligo repigmentation induced by UVB treatment. The HaCaT keratinocytes were used to mimic lesional condition and the PIG1 melanocytes as perilesional condition...
November 1, 2019: Experimental Cell Research
https://read.qxmd.com/read/31330146/essential-role-for-integrin-linked-kinase-in-melanoblast-colonization-of-the-skin
#18
JOURNAL ARTICLE
Melissa Crawford, Valerie Leclerc, Kevin Barr, Lina Dagnino
Melanocytes are pigment-producing cells found in the skin and other tissues. Alterations in the melanocyte lineage give rise to a plethora of human diseases, from neurocristopathies and pigmentation disorders to melanoma. During embryogenesis, neural crest cell subsets give rise to two waves of melanoblasts, which migrate dorsolaterally, hone to the skin, and differentiate into melanocytes. However, the mechanisms that govern colonization of the skin by the first wave of melanoblasts are poorly understood. Here we report that targeted inactivation of the integrin-linked kinase gene in first wave melanoblasts causes defects in the ability of these cells to form long pseudopods, to migrate, and to proliferate in vivo...
July 19, 2019: Journal of Investigative Dermatology
https://read.qxmd.com/read/31217845/screening-and-analysis-of-differentially-expressed-genes-of-human-melanocytes-in-skin-cells-mixed-culture
#19
JOURNAL ARTICLE
Xingyu Mei, Zhouwei Wu, Jie Huang, Yue Sun, Weimin Shi
BACKGROUND: This study aims to screen the key genes and possible signaling pathways involved in the differentiation and proliferation of human melanocytes (MCs) by in vitro culture of mixed skin cells. This will be helpful to further study the mechanisms and treatment strategies of pigment-related diseases such as vitiligo. METHODS: Mixed skin cells were obtained by digesting and separating normal human foreskin tissues. Ribonucleic acid (RNA) was extracted from sorting cells and high-throughput transcriptome sequencing was performed at different culture time points...
2019: American Journal of Translational Research
https://read.qxmd.com/read/31069610/cyclin-dependent-kinase-5-regulates-proliferation-migration-tyrosinase-activity-and-melanin-production-in-b16-f10-melanoma-cells-via-the-essential-regulator-p-creb
#20
JOURNAL ARTICLE
Xiuqing Li, Ruifang Wang, Junzhen Zhang, Shanshan Yang, Kaiyuan Ji, Bin Du, Xuexian Liu, Bo Liu, Shuhui Qi, Qiong Jia, Ruiwen Fan
Melanoma is an aggressive cancer with increasing incidence and a growing lifetime risk that arises from normal melanocytes or their precursors. A thorough understanding of the molecular mechanism of melanomagenesis and melanoma biology is essential for the diagnosis, prognostication, and therapy of melanoma. Cyclin-dependent protein kinase 5 (Cdk5) is one of the proteins highly expressed in B16-F10 melanoma cells that controls melanoma cell motility, invasiveness, and metastatic spread and might be a promising novel therapeutic target...
May 8, 2019: In Vitro Cellular & Developmental Biology. Animal
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