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Serum AND Eye

Jiao Hu, Yong-Zhong Jiang, Man Tang, Ling-Ling Wu, Hai-Yan Xie, Zhi-Ling Zhang, Dai-Wen Pang
Rapid and sensitive foodborne pathogen detection assay, which can be applied in multiple fields, is essential to timely diagnosis. Herein, we proposed a multi-signal readout lateral flow immunoassay for Salmonella typhimurium (S. typhi) detection. The assay employs colorimetric-fluorescent-magnetic nanospheres (CFMNs) as labels, which possess multi-functional: target separation and enrichment, multi-signal readout and two formats of quantitation. The assay for S. typhi detection involves magnetic separation and chromatography...
December 5, 2018: Analytical Chemistry
Adam A Vukovic, Meifawn D Poole, Erin F Hoehn, Alicia K Caldwell, Amanda C Schondelmeyer
We describe 2 cases of child maltreatment who presented as common pediatric conditions: preseptal cellulitis and gastroenteritis. The first case is an 8-year-old girl who presented with progressive right eye pain, swelling, and discharge. She was initially treated for preseptal cellulitis, but eye cultures ultimately grew Neisseria gonorrhoeae. Further investigation revealed sexual abuse by a male family member. The second case is a 2-year-old previously healthy girl who presented with 6 hours of emesis, lethargy, and abdominal pain...
November 26, 2018: Pediatric Emergency Care
Mai Kikumoto, Megumi Nonaka, Jun Takeshita, Tomohiko Ohshita, Hiroshi Yamashita
We report a case of neuromyelitis optica (NMO) that was diagnosed at the age of 90. After initially developing visual loss in the right eye, a patient was diagnosed with optic neuritis. Although treatment with methylprednisolone therapy provided relief, 3 months later she developed optic neuritis on the other side. Visual activity recovered after further administration of methylprednisolone. At the age of 91, she presented with muscle weakness of her left extremities. A cervical MRI revealed myelitis with a longitudinally extensive spinal cord lesion from the second to fifth cervical vertebrae...
November 29, 2018: Rinshō Shinkeigaku, Clinical Neurology
Masatoshi Hirayama
The lacrimal glands produce tears to support a healthy homeostatic environment on the ocular surface. The lacrimal gland dysfunction characteristic of dry eye disease causes ocular discomfort and visual disturbances and in severe cases can result in a loss of vision. The demand for adequate restoration of lacrimal gland function has been intensified due to advances in stem cell biology, developmental biology, and bioengineering technologies. In addition to conventional therapies, including artificial tears, tear alternatives (such as autologous serum eye drops) and salivary gland transplantation, a regenerative medicine approach has been identified as a novel strategy to restore the function of the lacrimal gland...
November 1, 2018: Investigative Ophthalmology & Visual Science
Akihiro Higuchi
Dry eye syndrome is a multifactorial condition on the tear and ocular surface. Autologous serum eye drop is an effective method for treating dry eye. Autologous serum eye drops are now widely used by specialists since a first report in 1975. The results of a systematic study showed that the efficacy of autologous serum eye drops remains ambiguous because its preparation methods and clinical application have not been standardized. To elucidate the efficacy of autologous serum eye drops, well-designed, large-scale, high-quality randomized controlled trials need to be conducted with standardized treatment and use...
November 1, 2018: Investigative Ophthalmology & Visual Science
Ikuko Toda
Post-LASIK dry eye is the most common postoperative dry eye after ophthalmic surgeries. The clinical signs of post-LASIK dry eye include positive vital staining of the ocular surface, decreased tear breakup time and Schirmer test values, reduced corneal sensitivity, and decreased functional visual acuity. The symptoms and signs usually last for about 1 month after LASIK. A small number of patients continue to experience symptoms more than 1 year postoperatively. It has been suggested that the loss of corneal innervation caused by flap-making is the major cause, affecting the corneal-lacrimal gland, corneal-blinking, and blinking-meibomian gland reflexes, resulting in decreased aqueous and lipid tear secretion and mucin expression...
November 1, 2018: Investigative Ophthalmology & Visual Science
Swati N Madhu, Kartik Kumar Jha, Annapoorna P Karthyayani, Devarshi Urvish Gajjar
Purpose: To develop an infectious keratitis model using caprine (goat) corneas and to investigate the expression of virulence factors during infection. Methods: Goat eyes were surface-sterilized and dissected, and the corneas were placed on an agarose-gelatin solid support (0.5% in phosphate-buffered saline) in a 12-well culture plate containing 10% fetal bovine serum-supplemented culture medium for 3 weeks. Cell viability tests (trypan blue and MTT) were performed on the cultured corneas...
October 2018: Journal of Ophthalmic & Vision Research
Aïcha Dede Djigo, Julie Bérubé, Solange Landreville, Stéphanie Proulx
The choroid of the eye is a vascularized and pigmented connective tissue lying between the retina and the sclera. Increasing evidence demonstrates that, beyond supplying nutrients to the outer retina, the different choroidal cells contribute to the retina's homeostasis, especially by paracrine signaling. However, the precise role of each cell type is currently unclear. Here, we developed a choroidal substitute using the self-assembly approach of tissue engineering. Retinal pigment epithelial (RPE) cells, as well as choroidal stromal fibroblasts, vascular endothelial cells and melanocytes, were isolated from human eye bank donor eyes...
November 23, 2018: Acta Biomaterialia
Tsung-Ting Tsai, Tse-Hao Huang, Chung-An Chen, Natalie Yi-Ju Ho, Yi-Ju Chou, Chien-Fu Chen
Lateral flow immunoassays (LFIAs) have wide application in point-of-care testing, particularly in resource-poor settings. To achieve signal amplification in a gold nanoparticle-based lateral flow assay without an additional procedure or the need for complex fabrication, a new and simple method was developed for using a "stacking pad" configuration that adds an additional membrane between the conjugation pad and test pad to the conventional AuNP-based LFIA format. This design helps to accumulate the antibody and antigen on the stacking pad, hence extending the antigen/antibody binding interactions to enhance the test's detection sensitivity...
November 23, 2018: Scientific Reports
Jixiang Wang, Jiangdong Dai, Yeqing Xu, Xiaohui Dai, Yunlei Zhang, Weidong Shi, Börje Sellergren, Guoqing Pan
Paper-based assays for detection of physiologically important species are needed in medical theranostics owning to their superiorities in point of care testing, daily monitoring, and even visual readout by using chromogenic materials. In this work, a facile test strip is developed for visual detection of a neurotransmitter dopamine (DA) based on dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs). The DE-MIPs, featured with tailor-made DA affinity and good anti-interference, exhibit DA concentration-dependent fluorescent colors, due to the variable ratios of dual-emission fluorescence caused by DA binding and quenching...
November 23, 2018: Small
N F Abd El-Aal, M A A Basha, A M Eid
Uveitis is one of the commonest causes of vision loss worldwide and its exact etiology is still not clarified in most patients. The current study is a trial to assess the efficacy of serum anti-Toxocara immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA) as a diagnostic tool for ocular toxocariasis (OT) and to detect OT prevalence and the associated ocular manifestations in sera of patients with uveitis. One hundred and twelve patients (62 females and 50 males) with uveitis were diagnosed by ophthalmologists, radiologists and rheumatologists according to ocular manifestations, laboratory and radiological investigations...
November 20, 2018: Journal of Helminthology
Sayo Ueda, Taichi Murakami, Hirokazu Ogino, Motokazu Matsuura, Masanori Tamaki, Seiji Kishi, Mike Hann, Yuko Toyoda, Kojiro Nagai, Yoshimi Bando, Hideharu Abe, Yasuhiko Nishioka, Toshio Doi
A 61-year-old man was diagnosed with sarcoidosis involving the lungs, eyes, parotid gland and extrathoracic lymph nodes complicated by chronic kidney injury and hypercalcemia. Kidney biopsy showed non-specific interstitial nephritis and nephrosclerosis. However, immunohistochemical staining of cell surface markers revealed a multinucleated giant macrophage surrounded by T-cells, suggesting granulomatous interstitial nephritis. Corticosteroid improved the kidney function, and reduced the serum levels of calcium and angiotensin-converting enzyme...
November 19, 2018: Internal Medicine
R Y Zhai, H Xu, X M Kong, Z J Wang
Objective: To observe the effect of 2% ganciclovir eye drops on Posner-Schlossman syndrome (PSS) patients with positive cytomegalovirus (CMV) in aqueous humor. Methods: Prospective study. The cases clinically diagnosed as PSS with positive CMV were enrolled from August 2017 to January 2018 at Department of Ophthalmology, Eye & ENT Hospital of Fudan University. All patients underwent aqueous and serum analysis for CMV by the enzyme linked immunosorbent assay and polymerase chain reaction. The history and clinical signs were noted, 2% ganciclovir eye drops were prescribed for topical use (4 times per day), and the patients were followed up...
November 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Jing Xue, Xue-Mei Wang, Yan Li, Li Zhu, Xiao-Ming Liu, Juan Chen, Shu-Hong Chi
IgG4-related disease (IgG4-RD) is an increasingly recognized pathological entity that tends to involve multiple organs with an elevated level of serum IgG4, which is easily misdiagnosed owing to sharing common clinical features with a variety of other diseases. Here, we report an interesting IgG4-RD case of a woman with progressive multi-organ involvement for over 19 years, started with swollen eyelids, dry eye and mouth, and polydipsia and hydruria. Imaging diagnosis revealed diffuse enlargement of the parotid glands, enlargement of the head of the pancreas, pulmonary infection and interstitial lung...
November 6, 2018: World Journal of Clinical Cases
Tsui-Hsuan Chang, Kuo-Hao Tung, Po-Wen Gu, Tzung-Hai Yen, Chao-Min Cheng
Paraquat intoxication is characterized by acute kidney injury and multi-organ failure, causing substantial mortality and morbidity. This study aims to develop a 2-in-1 paper-based analytical device to detect the concentrations of paraquat and creatinine in human serum, which can help clinicians diagnose patients with paraquat poisoning in a more rapid and geographically unrestricted manner. The procedure involves fabrication of a paper-based analytical device, i.e., printing of design on a filter paper, heating of wax-printed micro zone plates so as molten wax diffusing into and completely through the paper to the other side, forming hydrophobic boundaries that could act as detection zones for the paraquat colorimetric assay, and finally analysis using ImageJ software...
November 12, 2018: Micromachines
Cemile Ucgul Atilgan, Kadir Gokhan Atilgan, Pinar Kosekahya, Mehtap Caglayan, Selam Yekta Sendul, Pelin Yilmazbas
PURPOSE: To investigate the effect of microalbuminuria on macular thickness in patients with type-2 diabetes mellitus with no or mild diabetic retinopathy and to investigate the relationship between macular thickness and metabolic parameters. MATERIALS AND METHODS: Fifty eight eyes of 58 patients without diabetic retinopathy (group 1) in microalbuminuria stage, 42 eyes of 42 patients with mild diabetic retinopathy (group 2) in microalbuminuria stage, and 50 eyes of 50 patients without diabetic retinopathy and microalbuminuria (group 3) were included in this study...
November 8, 2018: European Journal of Ophthalmology
Megumi Shinzawa, Murat Dogru, Seika Den, Takehiro Ichijima, Kazunari Higa, Takashi Kojima, Noriyuki Seta, Takeshi Nomura, Kazuo Tsubota, Jun Shimazaki
PURPOSE: Sjögren syndrome (SS) is a chronic inflammatory autoimmune disease of the lacrimal and salivary glands. This study compared the concentrations of epidermal fatty-acid binding protein (E-FABP) in the saliva, serum, and tears of SS patients with dry eye and dry mouth, with those of healthy adults to investigate the usefulness of E-FABP as a diagnostic marker for SS. DESIGN: Prospective, observational case series. PARTICIPANTS: The subjects were 11 new patients with untreated Sjogren syndrome and 12 healthy control individuals...
November 4, 2018: International Journal of Molecular Sciences
John W Hinkle, Nidhi Relhan, Harry W Flynn
Background: Lipemia retinalis is a rare but known complication of elevated serum triglycerides. This case describes the clinical course of a diabetic patient who presented with lipemia retinalis and macular edema, which responded to systemic and local treatments. Case Report: A 40-year-old female with a history of type II diabetes mellitus, hypertriglyceridemia, and pancreatitis presented with decreased vision in the left eye. She had peripapillary and macular edema, intraretinal hemorrhages, and prominent exudates in the setting of lipemia retinalis due to type IV hypertriglyceridemia...
September 2018: Case Reports in Ophthalmology
Shafi Balal, Arit Udoh, Yannis Pappas, Erica Cook, Garry Barton, Ali Hassan, Karen Hayden, Rupert Richard Alexander Bourne, Sajjad Ahmad, Shahina Pardhan, Michael Harrison, Benjamin Sharma, Mohammad Wasil, Anant Sharma
INTRODUCTION: Patients with severe dry eye disease (DED) often have limited treatment options with standard non-surgical management focused on the use of artificial tears for lubrication and anti-inflammatory drugs. However, artificial tears do not address the extraordinary complexity of human tears. Crudely, human tears with its vast constituents is essentially filtered blood. Blood and several blood-derived products including autologous serum, have been studied as tear substitutes. This study proposes to test the use of whole, fresh, autologous blood obtained from a finger prick for treatment of severe DED...
October 31, 2018: BMJ Open
Toshiaki Hirakata, Hyeon-Cheol Lee, Mai Ohba, Kazuko Saeki, Toshiaki Okuno, Akira Murakami, Akira Matsuda, Takehiko Yokomizo
Allergic conjunctivitis (AC) is one of the most common ocular surface diseases in the world. In AC, T helper type 2 (Th 2) immune responses play central roles in orchestrating inflammatory responses. However, the roles of lipid mediators in the onset and progression of AC remain to be fully explored. Although previous reports have shown the beneficial effects of supplementation of ω-3 fatty acids in asthma or atopic dermatitis, the underlying molecular mechanisms are poorly understood. In this study, a diet rich in ω-3 fatty acids alleviated AC symptoms in both early and late phases without affecting Th 2 immune responses, but rather by altering the lipid mediator profiles...
November 1, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
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