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Serum AND Eye

Jacqueline Chua, Calvin Woon Loong Chin, Jimmy Hong, Miao Li Chee, Thu-Thao Le, Daniel Shu Wei Ting, Tien Yin Wong, Leopold Schmetterer
OBJECTIVE: Reduction in capillary density or rarefaction is a hallmark of essential hypertension. We measured the retinal capillary density using noninvasive optical coherence tomographic angiography (OCT-A) in adults with treated systemic hypertension and determined possible correlations with ambulatory blood pressure (BP) and renal parameters. METHODS: This observational cross-sectional study consisted of 153 normal eyes from 77 nondiabetic hypertensive adults [mean (SD) age, 58 (9) years; 49% women; 23% poorly controlled BP]...
August 14, 2018: Journal of Hypertension
Alfredo Iardino, Zain Amar, Yasir Ahmed
Haemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition, which is usually triggered by autoimmune disorders, viral infections and malignancy, like lymphomas. We present a 60-year-old Hispanic woman with a medical history of hypertension and systemic lupus erythematosus presenting with fever, generalised weakness and shortness of breath for 3 weeks. She was hypotensive on presentation, and a CT scan of abdomen/chest showed multiple irregular hepatic and splenic hypodense lesions. A liver biopsy revealed Classical Hodgkin lymphoma (cHL) with positive Epstein-Barr virus (EBV) staining that was later confirmed with high serum EBV DNA levels...
August 14, 2018: BMJ Case Reports
L Wen, X Sun, F H Wang, W Luo, K M Feng, Y Xue, G Y Mao, Y Wang, Y B Liang, G Zhai, C Xie, N L Wang
Objective: To investigate the attainment of therapeutic targets for glycosylated hemoglobin and blood pressure, ophthalmologic examination, diagnosis and treatment of diabetic retinopathy (DR) with type 2 diabetes mellitus. Methods: A cross-sectional study in type 2 diabetic patients was conducted in a community of Fushun between July 2012 and May 2013. Questionnaire, detailed ophthalmic examination and laboratory tests were completed to collect the information about sociodemographic and healthcare characteristics...
August 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Andrew W Francis, Ivy Zhu, Sidhiporn Borirakchanyavat, Daniel M Schwartz, David G Hwang
PURPOSE: To report a central retinal artery occlusion with cilioretinal artery sparing in a 48-year-old woman after laser-assisted in situ keratomileusis surgery. METHODS: Case history and clinical examination including best-corrected visual acuity, serum markers, slit-lamp biomicroscopy, indirect ophthalmoscopy, fundus photography, fluorescein angiography, and optical coherence tomography. RESULTS: A 48-year-old woman underwent routine laser-assisted in situ keratomileusis surgery in both eyes...
August 10, 2018: Retinal Cases & Brief Reports
Min-Kyung Kang, Eun-Jung Lee, Yun-Ho Kim, Dong Yeon Kim, Hyeongjoo Oh, Soo-Il Kim, Young-Hee Kang
Diabetes-associated visual cycle impairment has been implicated in diabetic retinopathy, and chronic hyperglycemia causes detrimental effects on visual function. Chrysin, a naturally occurring flavonoid found in various herbs, has anti-inflammatory, antioxidant, and neuroprotective properties. The goal of the current study was to identify the retinoprotective role of chrysin in maintaining robust retinoid visual cycle-related components. The in vitro study employed human retinal pigment epithelial (RPE) cells exposed to 33 mM of glucose or advanced glycation end products (AGEs) in the presence of 1⁻20 μM chrysin for three days...
August 8, 2018: Nutrients
Hirotaka Inoue, Naoki Shinojima, Ryuta Ueda, Shigetoshi Yano, Keizo Yamamoto, Norio Ishii, Motoyuki Igata, Junji Kawashima, Eiichi Araki, Hirotaka Iwase, Yoshiki Mikami, Akitake Mukasa
BACKGROUND: Thyroid-stimulating hormone (TSH)-secreting pituitary adenomas (TSHomas) are uncommon, and majority of the patients present with symptoms of hyperthyroidism. Herein, we report the first case of TSHoma with DTC that presented with visual disturbance without any clinical feature of hyperthyroidism. CASE DESCRIPTION: A 57-year-old man presented with left temporal hemianopsia of his left eye without any sign of hyperthyroidism. A mass lesion in the sellar and suprasellar region compressing the optic nerves was identified via magnetic resonance imaging...
August 7, 2018: World Neurosurgery
Chamara Jayamanne, Sajith Sandamal, Kasun Jayasundara, Mayoorathy Saranavabavananthan, Sachith Mettananda
BACKGROUND: Smith-Lemli-Opitz syndrome is a rare autosomal recessive disorder of cholesterol biosynthesis which is characterized by multiple congenital malformations and global developmental delay. Here we report the case of a 3-year-old, previously undiagnosed, child with Smith-Lemli-Opitz syndrome presenting with acute adrenal crisis, which is an extremely rare and atypical presentation of this disease. CASE PRESENTATION: A 3-year-old Sri Lankan Sinhalese boy without evidence of infection presented with circulatory collapse...
August 10, 2018: Journal of Medical Case Reports
Laura Gomes Nunes Melo, Paulo Henrique Morales, Karla Rezende Guerra Drummond, Deborah Conte Santos, Marcela Haas Pizarro, Bianca Senger Vasconcelos Barros, Tessa Cerqueria Lemos Mattos, André Araújo Pinheiro, Felipe Mallmann, Franz Schubert Lopes Leal, Fernando Korn Malerbi, Marilia Brito Gomes
BACKGROUND: Diabetic retinopathy is the leading cause of blindness in economically active populations. The aims of this study were to estimate the prevalence and to identify risk factors for diabetic retinopathy in patients with type 1 diabetes in Brazil. METHODS: This was a nationwide, cross-sectional study conducted between August 2010 and August 2014. The study included 1760 patients with type 1 diabetes. Patients underwent a standard questionnaire, clinical and laboratory analyses and were screened for diabetic retinopathy...
August 8, 2018: BMC Public Health
Hassan Boskabadi, Maryam Zakerihamidi, Ali Moradi, Mehdi Bakhshaee
Introduction: Hyperbilirubinemia is a common neonatal problem with toxic effects on the nervous system that can cause hearing impairment. This study was conducted to assess the risk factors for sensorineural hearing loss and other coexisting problems in icteric infants. Materials and Methods: In a case-control study, 200 term infants with bilirubin levels higher than 20 mg/dl admitted to the neonatal intensive care unit of Ghaem Hospital, Mashhad during 2007-2015 were investigated...
July 2018: Iranian Journal of Otorhinolaryngology
Angelica C Scanzera, Ellen Shorter
SIGNIFICANCE: Familial dysautonomia is a rare genetic disorder that affects the sensory and autonomic nervous systems. Affected individuals have decreased corneal sensation and can develop serious complications from neurotrophic keratitis. Scleral devices are an excellent option for the long-term management of patients with familial dysautonomia and neurotrophic keratitis. PURPOSE: In this series, we describe three patients with familial dysautonomia and classic ocular complications fit with scleral devices...
July 30, 2018: Optometry and Vision Science: Official Publication of the American Academy of Optometry
Anitza Fragas Quintero, Dervel Felipe Díaz Herrera, Dayamí Martín Alfonso, Yanelis Cruz Santana, Raisa Betancourt Torres, Lucy Montano Tamayo
Brucellosis is a serious infectious disease that causes significant economic losses in the livestock industry. Its early diagnosis allows an adequate disease control in cattle. DAVIH Laboratories designed a lateral flow immunochromatographic assay using protein A-colloidal gold as a detector reagent (LFIA-PA). The objective of this work was to compare the performance of this assay using protein G-colloidal gold (LFIA-PG) with its performance using protein A-colloidal gold as the detector reagent. The assays were carried out with 20 μL of serum and 130 μL of running buffer...
2018: Open Veterinary Journal
Nikolaos Trakas, Socrates J Tzartos
A rapid and simple immunostick immunosorbent ELISA has been developed for the diagnosis of myasthenia gravis. It is based on coating immunosticks with the major myasthenia gravis autoantigens acetylcholine receptor (AChR) or MuSK and it can be performed in <1 h, in a non-specialized examination place by non-skilled personnel. The resulting chromogen precipitate on the sticks, evaluated by eye, was proved a reliable indication of the presence of autoantigen-specific autoantibodies. Although this assay is mostly qualitative, compared to the results obtained from the gold-standard radioimmunoprecipitation assays, its specificity for anti-AChR antibodies was 99...
September 2018: Journal of Immunological Methods
Xuhua Tan, Yihe Chen, William Foulsham, Afsaneh Amouzegar, Takenori Inomata, Yizhi Liu, Sunil K Chauhan, Reza Dana
PURPOSE: In this study, we examine the expression of corneal epithelium-derived thrombospondin-1 (TSP-1) and its immunomodulatory functions in a validated murine model of dry eye disease (DED). METHODS: DED was induced in female C57BL/6 using a controlled environment chamber (CEC) for 14 days. mRNA and protein expression of TSP-1 by corneal epithelial cells was quantified using real-time PCR and flow cytometry. Corneal epithelial cells from either naïve or DED mice were cultured with bone marrow derived dendritic cells (BMDCs) in the presence of IFNγ for 48 h, and BMDC expression of MHC-II and CD86 was determined using flow cytometry...
July 25, 2018: Ocular Surface
Rachael L Niederer, Ahmed Al-Janabi, Sue L Lightman, Oren Tomkins-Netzer
PURPOSE: To examine whether measurement of serum angiotensin converting enzyme (ACE) is useful in diagnosing sarcoidosis in undifferentiated uveitis DESIGN: Evaluation of a diagnostic test. METHODS: Data collection was performed from 1035 consecutive subjects presenting with uveitis to Moorfields Eye Hospital undergoing measurement of serum ACE as part of baseline investigations for underlying systemic disease. The main outcome measures were sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of elevated serum ACE...
July 24, 2018: American Journal of Ophthalmology
Samantha Deianira Dattoli, Monica Baiula, Rossella De Marco, Andrea Bedini, Michele Anselmi, Luca Gentilucci, Santi Spampinato
BACKGROUND AND PURPOSE: Allergic conjunctivitis is an eye inflammation that involves the infiltration of immune cells into the conjunctiva via cell surface-adhesion receptors, such as integrin α4 β1 . These receptors interact with adhesion molecules expressed on the conjunctival endothelium and may be a target to treat this disease. We synthesised DS-70, a novel α/β-peptidomimetic α4 integrin antagonist, to prevent the conjunctival infiltration of immune cells and clinical symptoms in a model of allergic conjunctivitis...
July 26, 2018: British Journal of Pharmacology
Wei Lin, Konrad Buscher, Beibei Wang, Zhichao Fan, Nannan Song, Peng Li, Yingying Yue, Bingqing Li, Cuiling Li, Hongsheng Bi
Soluble CD83 (sCD83) is the extracellular domain of the membrane-bound CD83 molecule, and known for its immunoregulatory functions. Whether and how sCD83 participates in the pathogenesis of uveitis, a serious inflammatory disease of the eye that can cause visual disability and blindness, is unknown. By flow cytometry and imaging studies, we show that sCD83 alleviates experimental autoimmune uveitis (EAU) through a novel mechanism. During onset and recovery of EAU, the level of sCD83 rises in the serum and aqueous humor, and CD83+ leukocytes infiltrate the inflamed eye...
2018: Frontiers in Immunology
Shengjie Li, Danhui Li, Mingxi Shao, Wenjun Cao, Xinghuai Sun
PURPOSE: To evaluate the serum complement component (C)3 concentration in patients with primary open-angle glaucoma (POAG) and to investigate the association between C3 levels and POAG severity. METHODS: This study enrolled 190 consecutive POAG patients and 204 normal control subjects. A detailed eye and systematic examination, including intraocular pressure, gonioscopy, fundus photography, A-scan ultrasound, visual field testing, electrocardiograms, X-rays, liver function, renal function, infectious disease, etc, and measurement of serum C3 concentration by immunoturbidimetry, was performed...
July 20, 2018: Journal of Glaucoma
Sanjesh Singh, I Darren Grice, Ian R Peak, Thomas Frost, Geng Yue, Jennifer C Wilson
The Gram-negative bovine pathogen Moraxella bovis is a causative agent of Infectious bovine keratoconjunctivitis, 'pink-eye' that affects cattle. Here we report that strain L183/2 has the same capsular polysaccharide (CPS) of unsulfated chondroitin, as does strain Mb25, whereas strain Epp63 does not express CPS. NMR analysis of the oligosaccharides (OS) derived from the lipooligosaccharides (LOS) in these three strains by NMR has shown that strain Mb25 and Epp63 have the same OS structure with a terminal N-acetylgalactosamine ((1S)-GalaNAc) residue →4,6-linked...
July 19, 2018: Carbohydrate Research
Ying Yuan, Min Li, Chi Ho To, Thomas Chuen Lam, Peng Wang, Yunjie Yu, Qingzhong Chen, Xiaojun Hu, Bilian Ke
Purpose: Biomechanical properties changes and α-smooth muscle actin (α-SMA) overexpression are involved in myopia scleral remodeling. However, interactions between altered tissue biomechanics and cellular signaling that sustain scleral remodeling have not been well defined. We determine the mechanisms of mechanotransduction in the regulation of α-SMA expression during myopia scleral remodeling. Methods: Guinea pigs were used to establish a form-deprivation myopia (FDM) model...
July 2, 2018: Investigative Ophthalmology & Visual Science
Lang Zhang, Yali Yuan, Yun Zhang, Zhaoying Liu, Wencheng Xiao, Jinfang Nie, Jianping Li
In this paper, we describe an aptamer-based colorimetric assay (ABCA), which integrates enzyme-loaded microparticles for signal amplification with distance measurement for equipment-free quantitative readout. The distance measurement readout is on the basis of target-induced selective reduction in viscosity of reaction solution. Its utility is well demonstrated with inexpensive, sensitive, and selective detection of adenosine (model analyte) in buffer samples and real samples of human serum and urine with the naked eye...
February 28, 2018: ACS Omega
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