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Serum AND Eye

Dai Kakiuchi, Yoshikazu Taketa, Etsuko Ohta, Yasuhiro Fujikawa, Kyoko Nakano, Shoji Asakura, Satoru Hosokawa
Circulating miR-96-5p, -124-3p, and 183-5p have been reported as safety biomarkers for retinal toxicity. In the present research, 5 serum microRNAs (miRNAs), which are highly specific to and abundant in the retina, including the 3 miRNAs previously mentioned, were assessed in 3 different models of retinal toxicity. Distinct types of retinal lesions were induced in rats by a single dose of N-methyl-N-nitrosourea (MNU: 10, 30, and 50 mg/kg, i.p.), N-methyl-D-aspartate (NMDA: 200 nmol/eye, intravitreal injection), or sodium iodate (NaIO3 : 30 mg/kg, i...
October 15, 2018: Toxicology
Juan Yang, Jung Woo Park, Dejin Zheng, Ren-He Xu
Purpose: We generated universal corneal epithelial cells (CEC) from human embryonic stem cells (hESC) by genetically removing human leukocyte antigens (HLA) class I from the cell surface. Methods: The serum-free, growth factor-free, and defined medium E6 was used to differentiate hESC to CEC. Decellularized murine corneas were recellularized with hESC-derived CEC. Using CRISPR/Cas9, β-2-microglobulin ( B2M ) was deleted in hESC to block the assembly of HLA class-I antigens on the cell surface to generate B2M -/- CEC...
September 2018: Translational Vision Science & Technology
Koji Kanda, Kei Takayama, Toshio Enoki, Masaru Takeuchi
Objective: Penicillium species are abundant, generally saprophytic, and widely distributed in the environment. On rare occasions, they cause endophthalmitis in immunocompromised patients or in immunocompetent patients with uncontrolled diabetic mellitus or immunosuppressive agents. We report a case of endophthalmitis caused by Penicillium species in an immunocompetent patient without any disorders. Case presentation: A 67-year-old woman presented with ocular pain, hyperemia, and blurred vision in her right eye...
2018: International Medical Case Reports Journal
M Amin Farahmand Nejad, Forough Ghasemi, M Reza Hormozi-Nezhad
Owing to its dual role as a hormone and neurotransmitter, norepinephrine (NE) detection is of great significance to biomedical diagnosis. In the present work, we have explored intense green fluorescence of poly (norepinephrine) (PNE) nanoparticles synthesized by oxidizing NE in alkaline condition, in combination with red fluorescent bovine serum albumin-stabilized gold nanoclusters (BSA-AuNCs) for naked-eye detection of NE. The effect of sodium hydroxide on the emission behavior of NE was studied. The surface morphology and optical properties of PNE nanoparticles were characterized by UV-Vis, fluorescence, FTIR, Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) techniques...
December 18, 2018: Analytica Chimica Acta
Sandra Kuehn, Wilhelm Meißner, Pia Grotegut, Carsten Theiss, H Burkhard Dick, Stephanie C Joachim
The glial protein S100B, which belongs to a calcium binding protein family, is up-regulated in neurological diseases, like multiple sclerosis or glaucoma. In previous studies, S100B immunization led to retinal ganglion cell (RGC) loss in an experimental autoimmune glaucoma (EAG) model. Now, the direct degenerative impact of S100B on the retina and optic nerve was evaluated. Therefore, 2 μl of S100B was intravitreally injected in two concentrations (0.2 and 0.5 μg/μl). At day 3, 14 and 21, retinal neurons, such as RGCs, amacrine and bipolar cells, as well as apoptotic mechanisms were analyzed...
2018: Frontiers in Cellular Neuroscience
Bailu Du, Yue Tao, Jing Ma, Xing Weng, Yanping Gong, Yang Lin, Nan Shen, Xi Mo, Qing Cao
The incidence of sparganosis, a parasitic disease caused by plerocercoid larvae of the genus Spirometra, has gradually risen worldwide (especially in remote areas) in recent years. Pulmonary and pleural sparganosis, as well as other sites of infestation, including the subcutaneous tissues, the abdominal viscera, brain and eyes, has been reported. In clinical practice, due to the atypical signs and symptoms and limited laboratory approaches for the specific detection of sparganum, sparganosis is often misdiagnosed...
October 10, 2018: Infection, Genetics and Evolution
Katherine Chuere Nunes Duarte, Thaís Torres Soares, Angela Maria Paiva Magri, Lívia Assis Garcia, Luciana Le Sueur-Maluf, Ana Cláudia Muniz Renno, Gláucia Monteiro de Castro
There are no effective therapies for remyelination. Low-level laser therapy (LLLT) has been found advantageous in neurogenesis promotion, cell death prevention, and modulation of inflammation in central and peripheral nervous system models. The purpose of this study was to analyse LLLT effects on cuprizone-induced demyelination. Mice were randomly distributed into three groups: Control Laser (CTL), Cuprizone (CPZ), and Cuprizone Laser (CPZL). Mice from CPZ and CPZL groups were exposed to a 0.2% cuprizone oral diet for four complete weeks...
October 3, 2018: Journal of Photochemistry and Photobiology. B, Biology
Eduardo Anitua, María de la Fuente, Francisco Muruzabal, Ronald Mauricio Sánchez-Ávila, Jesús Merayo-Lloves, Mikel Azkargorta, Felix Elortza, Gorka Orive
PURPOSE: The main objective of this study is to compare the protein expression of human keratocytes treated with Plasma rich in growth factors (PRGF) or autologous serum (AS) and previously induced to myofibroblast by TGF-β1 treatment. METHODS: Blood from healthy donor was collected and processed to obtain AS and PRGF eye drops. Blood derivates were aliquoted and stored at -80°C until use. Keratocyte cells were pretreated for 60 minutes with 2.5 ng/ml TGF-β1...
2018: PloS One
Xiangling Li, Zhengqi Lu, Yanqiang Wang
BACKGROUND: Besides CSF-flow obstruction, syringomyelia is associated with inflammatory spinal cord lesions. However, syringomyelia-like syndrome concomitant with neuromyelitis optica spectrum disorder (NMOSD) and primary Sjogren's syndrome (pSS) is extremely rare. Here, we would like to report a case of a patient with syringomyelia-like syndrome in NMOSD complicated with Sjogren's Syndrome. CASE PRESENTATION: A 64-old male Han Chinese, presented with three episodes of acute demyelinating processes in the central nervous system within 5 years...
October 9, 2018: BMC Neurology
Katarzyna Oszajca, Maciej Szemraj, Janusz Szemraj, Piotr Jurowski
BACKGROUND: Age-related macular degeneration is a progressive eye disease affecting the macula and causing acute visual loss particularly in elder people. The aim of the study was an attempt to discern an influence of expression levels and functional genetic polymorphisms of selected genes related to the extracellular matrix turnover or neovascularization on age-related macular degeneration occurrence and progression. METHODS: We conducted a case-control study of 200 polish patients with recognized age-related macular degeneration (dry and wet) and compared the results with those obtained from matched 100 healthy control subjects...
October 5, 2018: Ophthalmic Genetics
Xudong Li, Shuhong Jia, Zhi Zhou, Yi Jin, Xiangfei Zhang, Chunlei Hou, Wenjing Zheng, Pei Rong, Jinsong Jiao
Idiopathic rapid eye movement sleep behavior disorder (iRBD) likely represents the prodromal stage of synucleinopathy. The present study investigated how levels of serum uric acid (UA) affect cognition and motor function in patients with iRBD. A total of 42 patients with iRBD and 45 healthy controls were included. All participants were given cognitive tests and motor assessments. Serum UA concentrations were measured. The patients were further divided into two groups (high or low UA) according to serum UA level...
October 3, 2018: Journal of Neural Transmission
Guobin Mao, Mingyuan Du, Xinxin Wang, Xinghu Ji, Zhike He
In this work, a simple and novel ratiometric fluorescence method based on ROX-DNA-functionalized CdZnTeS quantum dots (QDs) was developed for the detection of dopamine (DA) and tyrosinase (TYR). A ratiometric fluorescent probe was constructed by binding phosphorothioate DNA to the metal ions of QDs, which is a modification-free and low-cost method. DA was easily oxidized to DA quinone under the catalysis of TYR by dissolved O2, which effectively quenched the fluorescence of the QDs. Strong linear correlations were achieved for TYR in the range of 10...
October 4, 2018: Analyst
Jost B Jonas, Wen Bin Wei, Liang Xu, Ya Xing Wang
PURPOSE: Systemic inflammation is potentially associated with ocular diseases such as late age-related macular degeneration (AMD). Using the serum concentration of high-sensitive C-reactive protein (hs-CRP) as surrogate of systemic inflammation, we examined potential associations between the serum hs-CRP concentration and the presence and degree of eye diseases. METHODS: The population-based Beijing Eye Study included 3468 Chinese individuals. The study participants underwent a standardized interview and a detailed ophthalmic examination...
2018: PloS One
Priscilla Griffo, Vilma V S T Viana, Sandra G Pasoto, Elaine P Leon, Eloisa Bonfa
BACKGROUND AND OBJECTIVE: Primary Sjögren syndrome (pSS) is a systemic autoimmune rheumatic disease that particularly affects exocrine glands. Dry eye is one of the most important features of this syndrome, and a recent study reported reduced deoxyribonuclease I (DNase I) activity in the tear of patients with dry eye. We therefore postulated that patients with pSS might have antibodies targeting DNAse I. METHODS: We have evaluated in a cross-sectional study 85 patients with pSS (2002 American-European Consensus Group Criteria), 50 rheumatoid arthritis (RA) patients (1987 American College of Rheumatology Criteria) without sicca symptoms, and 88 healthy volunteers...
September 27, 2018: Journal of Clinical Rheumatology: Practical Reports on Rheumatic & Musculoskeletal Diseases
Sumet Supalaset, Napaporn Tananuvat, Saipin Pongsatha, Winai Chaidaroon, Somsanguan Ausayakhun
PURPOSE: To evaluate the efficacy, safety and quality of life (QOL) of transdermal androgen in treatment of dry eye patients associated with androgen deficiency DESIGN: Randomized controlled trial SETTING: A tertiary eye center in Northern Thailand METHODS: Fifty patients with dry eye were randomized to receive transdermal androgen or placebo for four weeks. Main outcome measures were symptoms and signs of dry eye. Serum level of sex-hormone and QOL questionnaires were also evaluated at the baseline and after treatment...
September 27, 2018: American Journal of Ophthalmology
Xavier Vallès, M Henar Alonso, Juan Francisco López-Caleya, Virginia Díez-Obrero, Trinidad Dierssen-Sotos, Virginia Lope, Ana Molina-Barceló, María Dolores Chirlaque, José Juan Jiménez-Moleón, Guillermo Fernández Tardón, Jesús Castilla, Pilar Amiano, Rocío Capelo, Gemma Castaño-Vinyals, Elisabet Guinó, Antonio José Molina de la Torre, Conchi Moreno-Iribas, Beatriz Pérez Gómez, Nuria Aragonés, Javier Llorca, Vicente Martín, Manolis Kogevinas, Marina Pollán, Victor Moreno
OBJECTIVES: To explore the association of colorectal cancer with environmental solar radiation and sun exposure behavior, considering phenotypic variables (eye color, hair color and skin phenotype), dietary intake of vitamin D and calcium, and socio-demographic factors. STUDY DESIGN: Multicenter population-based frequency matched case-control study in Spain (MCC-Spain), with 2140 CRC cases and 3950 controls. METHODS: Data were obtained through personal interviews using a structured epidemiological questionnaire that included socio-demographic data, residential history, environmental exposures, behavior, phenotypic and dietary information...
September 25, 2018: Environment International
Tanuj Dada, Deepti Mittal, Kuldeep Mohanty, Muneeb A Faiq, Muzaffer A Bhat, Raj K Yadav, Ramanjit Sihota, Talvir Sidhu, Thirumurthy Velpandian, Mani Kalaivani, Ravindra M Pandey, Ying Gao, Bernhard A Sabel, Rima Dada
BACKGROUND: Reducing intraocular pressure (IOP) in primary open angle glaucoma (POAG) is currently the only approach to prevent further optic nerve head damage. However, other mechanisms such as ischemia, oxidative stress, glutamate excitotoxicity, neurotrophin loss, inflammation/glial activation, and vascular dysregulation are not addressed. Because stress is a key risk factor affecting these mechanisms, we evaluated if mindfulness based stress reduction (MBSR) can lower IOP and normalize typical stress biomarkers...
September 25, 2018: Journal of Glaucoma
Cheng-Kuan Su, Jo-Chin Chen
To substantially simplify the fabrication of analytical devices for rapid screening tests, in this study we employed multi-material fused deposition modeling-type three-dimensional printing (3DP) and two functionalized thermoplastic filaments-acrylonitrile butadiene styrene (ABS) incorporating peroxidase-mimicking iron oxide (Fe3 O4 ) nanoparticles and polyvinyl alcohol (PVA) infiltrated with the chromogenic substrate o-phenylenediamine (OPD)-for the one-step manufacture of enzyme/substrate-incorporated multi-well plates...
December 7, 2018: Analytica Chimica Acta
Shuzhen Song, Jianping Wu, Shengguo Zhao, David Paul Casper, Liping Zhang, Bin He, Xia Lang, Cailian Wang, Xuyin Gong, Fei Wang, Lishan Liu
Energy is a key factor regulated by the neuroendocrine system. The objective of this study was to compare growth performance, serum biochemical indices, carcass traits, meat quality, and nutrient composition of lambs fed restricted metabolizable energy (ME) intakes to mimic the seasonal changes of the natural grasslands of northwest China. Nineteen male Dorper × Small Tailed Han lambs were assigned to treatments, control (CON) fed at 1.0 MJ/W0.75 × d-1 throughout the 180 d study, and restricted ME (RES) fed by sequentially restricting ME every 30 d (0...
September 29, 2018: Journal of Animal Science
Narumon Keorochana, Wasamon Intaraprasong, Raveewan Choontanom
Objective: The aim of this study was to determine the prevalence of Herpesviridae family in aqueous humor and the prevalence of antibodies against Herpesviridae family in serum. Methods: Participants undergoing cataract surgery were included in the study. Serum for viral serology including herpes simplex virus (HSV), varicella zoster virus (VZV), Epstein-Barr virus (EBV), and cytomegalovirus (CMV) was collected. Aqueous humor specimen was tapped for PCR analysis...
2018: Clinical Ophthalmology
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