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https://www.readbyqxmd.com/read/29150936/the-roles-of-wnt-%C3%AE-catenin-pathway-in-tissue-development-and-regenerative-medicine
#1
REVIEW
Maryam Majidinia, Javad Aghazadeh, Rana Jahanban-Esfahlani, Bahman Yousefi
Regenerative medicine is a translational field which combines tissue engineering and molecular biology to construct spare organs or help injured or defective tissues to regenerate or restore their normal functions. This is particularly important with specific organs such as heart, central nervous system, retina or limbs which possess very limited regenerative capacity. As such, regenerative medicine has received peculiar attention in the last decade. In this regard, Wnt/β-catenin signaling pathway has been subject to intensive research, since it plays many essential roles in the regulation of the progenitor cell fate, developmental decisions, proliferation during embryonic development, and adult tissue homeostasis...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29130158/crispr-in-the-retina-evaluation-of-future-potential
#2
Galaxy Y Cho, Sally Justus, Jesse D Sengillo, Stephen H Tsang
Clustered regularly interspaced short palindromic repeats (CRISPR) has been gaining widespread attention for its ability for targeted genome surgery. In treating inherited retinal degenerations, gene therapies have had varied results; the ones effective in restoring eye sight are limited by transiency in its effect. Genome surgery, however, is a solution that could potentially provide the eye with permanent healthy cells. As retinal degenerations are irreversible and the retina has little regenerative potential, permanent healthy cells are vital for vision...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29128844/a-biodegradable-scaffold-enhances-differentiation-of-embryonic-stem-cells-into-a-thick-sheet-of-retinal-cells
#3
Deepti Singh, Shao-Bin Wang, Tina Xia, Laurel Tainsh, Maryam Ghiassi-Nejad, Tao Xu, Shaomin Peng, Ron A Adelman, Lawrence J Rizzolo
Retinal degeneration is a leading cause of blindness in developed countries. Stem cells can be differentiated into retinal organoids to study mechanisms of retinal degeneration, develop therapeutic agents, and potentially serve as replacement tissues. The spherical nature of these retinoids limits their utility, because the investigator lacks ready access to both sides of the neo-tissue. For tissue-replacement, spherical retinoids are unable to interact simultaneously with the host retinal pigment epithelium and remaining neurosensory retina...
October 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/29103970/detection-of-retinal-pigment-epithelium-specific-antibody-in-ipsc-derived-retinal-pigment-epithelium-transplantation-models
#4
Sunao Sugita, Kenichi Makabe, Shota Fujii, Yuko Iwasaki, Hiroyuki Kamao, Takashi Shiina, Kazumasa Ogasawara, Masayo Takahashi
Antibody-mediated rejection is characterized by donor-specific antibody produced by B cells. However, to our knowledge, B cell invasion and antibody in the inflamed retina after transplantation of retinal pigment epithelial (RPE) cells has not been reported. To determine if RPE transplantation could be performed using allografts, we established in vivo immune rejection models with induced pluripotent stem cell (iPSC)-RPE allografts and determined whether RPE-specific antibody could be detected in these models...
November 14, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29057815/further-insights-into-the-ciliary-gene-and-protein-kiz-and-its-murine-ortholog-plk1s1-mutated-in-rod-cone-dystrophy
#5
Said El Shamieh, Cécile Méjécase, Matteo Bertelli, Angélique Terray, Christelle Michiels, Christel Condroyer, Stéphane Fouquet, Maxime Sadoun, Emmanuelle Clérin, Binqian Liu, Thierry Léveillard, Olivier Goureau, José-Alain Sahel, Isabelle Audo, Christina Zeitz
We identified herein additional patients with rod-cone dystrophy (RCD) displaying mutations in KIZ, encoding the ciliary centrosomal protein kizuna and performed functional characterization of the respective protein in human fibroblasts and of its mouse ortholog PLK1S1 in the retina. Mutation screening was done by targeted next generation sequencing and subsequent Sanger sequencing validation. KIZ mRNA levels were assessed on blood and serum-deprived human fibroblasts from a control individual and a patient, compound heterozygous for the c...
October 18, 2017: Genes
https://www.readbyqxmd.com/read/29050186/-influent-factors-for-treating-procedure-of-cytomegalovirus-retinitis-after-allogeneic-bone-marrow-hematopoietic-stem-cell-transplantation
#6
H Miao, J Hou
Objective: To explore clinical and laboratory factors that influencing treating procedure of cytomegalovirus retinitis (CMVR) after allogeneic bone marrow hematopoietic stem cell transplantation (HSCT). Methods: This is a retrospective case series study. A total of 9 CMVR patients (15 eyes) between January 2016 and March 2017 were included in this study. All patients received intravenous or oral ganciclovir, together with intravitreous injection of ganciclovir alone or combined with foscanet sodium. One day before the first injection, aqueous humor samples from the affected eyes were collected, and CMV-DNA and interleukin-8 (IL-8) level were examined...
October 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/29049728/surgically-induced-focal-retinal-detachment-does-not-cause-detectable-sd-oct-retinal-changes-in-normal-human-retina
#7
MULTICENTER STUDY
Kaitlin Kogachi, Jeremy D Wolfe, Amir H Kashani
Purpose: Induction of focal retinal detachment (RD) for subretinal delivery of stem cells and gene therapy is increasingly common. In order to determine if this procedure has an adverse impact on the retina, we use spectral-domain optical coherence tomography (SD-OCT) to evaluate the pre- and postoperative retinal anatomy of the incidentally detached normal retina surrounding large submacular hemorrhages (SMH) during surgical displacement procedures. Methods: Retrospective, observational study of human subjects with monocular SMH evaluated before and after surgical displacement using clinical exam, fundus photography, and SD-OCT...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29047024/generation-of-photoreceptor-precursors-from-mouse-embryonic-stem-cells
#8
Amy Q Lu, Colin J Barnstable
Embryonic stem cell (ESC) differentiation can be used to model development and to produce transplantable cells of the desired phenotype. ESCs can reproducibly generate retinal cells but the derivation of photoreceptor precursors is variable and depends on an array of exogenous factors and intrinsic cell-cell interactions. In this work, we have defined the use of exogenous signaling factors, dissociation, and adherent versus 3-dimensional (3D) conditions on the derivation of retinal cells from pluripotent mouse ESCs...
October 18, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/29044808/neuroprotective-effects-of-human-mesenchymal-stem-cells-and-platelet-derived-growth-factor-on-human-retinal-ganglion-cells
#9
Andrew Osborne, Julie Sanderson, Keith R Martin
Optic neuropathies such as glaucoma occur when retinal ganglion cells (RGCs) in the eye are injured. Strong evidence suggests mesenchymal stem cells (MSCs) could be a potential therapy to protect RGCs, however little is known regarding their effect on the human retina. We, therefore, investigated if human MSCs (hMSCs), or platelet-derived growth factor (PDGF) as produced by hMSC, could delay RGC death in a human retinal explant model of optic nerve injury. Our results showed hMSCs and the secreted growth factor PDGF-AB could substantially reduce human RGC loss and apoptosis following axotomy...
October 17, 2017: Stem Cells
https://www.readbyqxmd.com/read/29042190/hematopoietic-stem-progenitor-involvement-in-retinal-microvascular-repair-during-diabetes-implications-for-bone-marrow-rejuvenation
#10
Ashay D Bhatwadekar, Yaqian Duan, Maria Korahw, Jeffrey S Thinschmidt, Ping Hu, Sameer P Leley, Sergio Caballero, Lynn Shaw, Julia Busik, Maria B Grant
The widespread nature of diabetes affects all organ systems of an individual including the bone marrow. Long-term damage to the cellular and extracellular components of the bone marrow leads to a rapid decline in the bone marrow-hematopoietic stem/progenitor cells (HS/PCs) compartment. This review will highlight the importance of bone marrow microenvironment in maintaining bone marrow HS/PC populations and the contribution of these key populations in microvascular repair during the natural history of diabetes...
October 14, 2017: Vision Research
https://www.readbyqxmd.com/read/29025379/survival-and-migration-of-adipose-derived-stem-cells-transplanted-in-the-injured-retina
#11
Hamid Aboutaleb Kadkhodaeian, Taki Tiraihi, Hamid Ahmadieh, Hossein Ziaei Ardakani, Narsis Daftarian, Taher Taheri
OBJECTIVES: Transplantation of stem cells is one of the approaches to treat retinal diseases. Our objective was to determine whether adipose-derived stem cell transplant can survive and migrate in the injured retina using a sodium iodate model for the pigmented retinal epithelium injury. MATERIALS AND METHODS: The adipose-derived stem cells were isolated from male albino Sprague-Dawley rats and labeled with DiI so as to track the transplants in the subretinal space...
October 12, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28979193/intraocular-injection-of-es-cell-derived-neural-progenitors-improve-visual-function-in-retinal-ganglion-cell-depleted-mouse-models
#12
Mundackal S Divya, Vazhanthodi A Rasheed, Tiffany Schmidt, Soundararajan Lalitha, Samer Hattar, Jackson James
Retinal ganglion cell (RGC) transplantation is a promising strategy to restore visual function resulting from irreversible RGC degeneration occurring in glaucoma or inherited optic neuropathies. We previously demonstrated FGF2 induced differentiation of mouse embryonic stem cells (ESC) to RGC lineage, capable of retinal ganglion cell layer (GCL) integration upon transplantation. Here, we evaluated possible improvement of visual function by transplantation of ES cell derived neural progenitors in RGC depleted glaucoma mice models...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28962643/long-term-safety-of-human-retinal-progenitor-cell-transplantation-in-retinitis-pigmentosa-patients
#13
Yong Liu, Shao Jun Chen, Shi Ying Li, Ling Hui Qu, Xiao Hong Meng, Yi Wang, Hai Wei Xu, Zhi Qing Liang, Zheng Qin Yin
BACKGROUND: Retinitis pigmentosa is a common genetic disease that causes retinal degeneration and blindness for which there is currently no curable treatment available. Vision preservation was observed in retinitis pigmentosa animal models after retinal stem cell transplantation. However, long-term safety studies and visual assessment have not been thoroughly tested in retinitis pigmentosa patients. METHODS: In our pre-clinical study, purified human fetal-derived retinal progenitor cells (RPCs) were transplanted into the diseased retina of Royal College of Surgeons (RCS) rats, a model of retinal degeneration...
September 29, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28955745/bioengineered-bruch-s-like-extracellular-matrix-promotes-retinal-pigment-epithelial-differentiation
#14
Samuel McLenachan, Erwei Hao, Dan Zhang, Ling Zhang, Michael Edel, Fred Chen
In the eye, the retinal pigment epithelium (RPE) adheres to a complex protein matrix known as Bruch's membrane (BrM). The aim of this study was to provide enriched conditions for RPE cell culture through the production of a BrM-like matrix. Our hypothesis was that a human RPE cell line would deposit an extracellular matrix (ECM) resembling BrM. The composition and structure of ECM deposited by ARPE19 cells (ARPE19-ECM) was characterized. To produce ARPE19-ECM, ARPE19 cells were cultured in the presence dextran sulphate...
July 2017: Biochemistry and Biophysics Reports
https://www.readbyqxmd.com/read/28936466/mouse-m%C3%A3-ller-cell-isolation-and-culture
#15
Xiao Liu, Luosheng Tang, Yongqing Liu
Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Müller cells.
August 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28930148/elongation-of-axon-extension-for-human-ipsc-derived-retinal-ganglion-cells-by-a-nano-imprinted-scaffold
#16
Tien-Chun Yang, Jen-Hua Chuang, Waradee Buddhakosai, Wen-Ju Wu, Chen-Ju Lee, Wun-Syuan Chen, Yi-Ping Yang, Ming-Chia Li, Chi-Hsien Peng, Shih-Jen Chen
Optic neuropathies, such as glaucoma and Leber's hereditary optic neuropathy (LHON) lead to retinal ganglion cell (RGC) loss and therefore motivate the application of transplantation technique into disease therapy. However, it is a challenge to direct the transplanted optic nerve axons to the correct location of the retina. The use of appropriate scaffold can promote the proper axon growth. Recently, biocompatible materials have been integrated into the medical field, such as tissue engineering and reconstruction of damaged tissues or organs...
September 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28928775/assessment-of-safety-and-functional-efficacy-of-stem-cell-based-therapeutic-approaches-using-retinal-degenerative-animal-models
#17
REVIEW
Tai-Chi Lin, Magdalene J Seiler, Danhong Zhu, Paulo Falabella, David R Hinton, Dennis O Clegg, Mark S Humayun, Biju B Thomas
Dysfunction and death of retinal pigment epithelium (RPE) and or photoreceptors can lead to irreversible vision loss. The eye represents an ideal microenvironment for stem cell-based therapy. It is considered an "immune privileged" site, and the number of cells needed for therapy is relatively low for the area of focused vision (macula). Further, surgical placement of stem cell-derived grafts (RPE, retinal progenitors, and photoreceptor precursors) into the vitreous cavity or subretinal space has been well established...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28903152/nuclear-respiratory-factor-1-nrf-1-regulates-transcription-of-the-cxc-receptor-4-cxcr4-in-the-rat-retina
#18
Pei Chen, Xiaoxiao Cai, Ying Yang, Zhao Chen, Jin Qiu, Na Yu, Mingjun Tang, Qiyun Wang, Jian Ge, Keming Yu, Jing Zhuang
Purpose: The CXC receptor 4 (CXCR4) is required for various physiologic and pathologic processes in the eye, including stem cell trafficking, neuronal development, immune responses, and ocular neovascularization. Here, we used the rat retina models to determine the mechanisms driving CXCR4 transcription. Methods: The expression pattern of CXCR4 and nuclear respiratory factor-1 (NRF-1) were profiled in the rat retina during the course of development. Chromatin immunoprecipitation (CHiP) assay determined the transcriptional mechanism of CXCR4 in rat retina...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28902341/bilateral-retinal-detachments-after-intravitreal-injection-of-adipose-derived-stem-cells-in-a-patient-with-exudative-macular-degeneration
#19
Steven S Saraf, Matthew A Cunningham, Ajay E Kuriyan, Sarah P Read, Philip J Rosenfeld, Harry W Flynn, Thomas A Albini
A 77-year-old woman with exudative macular degeneration underwent bilateral intravitreal injections of "stem cells" at a clinic in Georgia. One month and 3 months after injection, she developed retinal detachments in the left and right eyes, respectively. Increased awareness within the medical community of such poor outcomes is critical so that clinics offering untested practices that have been shown to be potentially harmful to patients can be identified and brought under U.S. Food and Drug Administration oversight...
September 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28900179/microrna-28-potentially-regulates-the-photoreceptor-lineage-commitment-of-m%C3%A3-ller-glia-derived-progenitors
#20
Hong-Pei Ji, Yu Xiong, Wei-Tao Song, En-Dong Zhang, Zhao-Lin Gao, Fei Yao, Tao Su, Rong-Rong Zhou, Xiao-Bo Xia
Retinal degenerative diseases ultimately result into irreversible photoreceptor death or loss. At present, the most promising treatment for these diseases is cell replacement therapy. Müller glia are the major glia in the retina, displaying cardinal features of retinal progenitor cells, and can be candidate of seed cells for retinal degenerative diseases. Here, mouse retinal Müller glia dissociated and cultured in vitro amplified and were dedifferentiated into Müller glia-derived progenitors (MGDPs), demonstrating expression of stem/progenitor cell markers Nestin, Sox2 and self-renewal capacity...
September 12, 2017: Scientific Reports
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