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Min Zeng, Yulin Luo, Chunrong Xu, Rong Li, Ni Chen, Xin Deng, Dan Fang, Liqun Wang, Jianbo Wu, Mao Luo
Platelets (PLTs) - endothelial cells (ECs) interaction appears to contribute to phenotypic transition of VSMCs that play an important role in the physiological and pathological process of vascular complications in Type 2 diabetes (DM2). However, the precise mechanisms by which interactions between PLTs and ECs affect VSMCs phenotype, have largely remained unclear. We determined the effect of diabetic PLTs-ECs interaction to influence VSMCs migration, proliferation and phenotypic transformation in triple cell co-culture models using the qRT-PCR, western blot, fluorescence microscopy, wound scratch assays, CCK-8 assays, and gelatine zymography assays...
December 5, 2018: American Journal of Physiology. Cell Physiology
Ayhan Aköz, Kenan Ahmet Türkdoğan, Nesibe Kahraman Çetin, Selen Kum, Ali Duman, Mevlüt Türe, Ahmet Ender Demirkıran
BACKGROUND: The objective of the current study was to investigate the value of the ischemic biomarkers endothelial cell-specific molecule-1 (endocan) and signal peptide-CUB-EGF domain-containing protein-1 (SCUBE-1) in the diagnosis and assessment of earlystage and irreversible damage in acute mesenteric ischemia. METHODS: An experimental mesenteric ischemia reperfusion model was designed using 54 rats. Nine groups were created: Three sham groups [Groups I (30th minute), IV (2nd hour), and VII (6th hour)], in which only blood and tissue specimens were sampled; 3 ischemia groups [Groups II (30th minute), V (2nd hour), and VIII (6th hour)], in which blood and tissue specimens were sampled after ligation of the superior mesenteric artery (SMA); and 3 reperfusion groups [Groups III (30th minute), VI (2nd hour), and IX (6th hour)], in which blood and tissue specimens were sampled after declamping the SMA and reperfusion for 1 hour...
November 2018: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
Ayano Ishizeki, Tomonori Kishino, Sawano Ogura, Haruna Kuga, Yuki Masai, Keiichiro Harashima, Satoshi Nakajima, Junichi Otaki, Hiroaki Ohnishi, Takashi Watanabe
Hemodynamics is subject to change after eating meals, which may be related to various postprandial physical statuses such as hypotension or daytime sleepiness. Previous studies have shown that blood flow in the superior mesenteric artery (SMA) increases after meals, but conflicting results have been reported regarding blood flow in the common carotid artery (CCA). In those studies, the fasting interval before the meal was not taken into account. For example, eating breakfast shortly before lunch may affect hemodynamics in these vessels...
December 4, 2018: Clinical Physiology and Functional Imaging
Mei-Yin Lin, Yi-Jhan Tseng, Chia-Hsiung Cheng
Inhibition, the ability to suppress irrelevant information, thoughts or movements, is crucial for humans to perform context-appropriate behaviors. It was suggested that declined cognitive performance in older adults might be attributed to inhibitory deficiencies. Although previous studies have shown an age-associated reduction in inhibitory ability, the understanding regarding its cortical spatiotemporal maps remained limited. Thus, we used a whole-head magnetoencephalography (MEG) to elucidate the age effects on response inhibition, and to explore the brain activation differences in high- and low-performing seniors...
2018: Frontiers in Aging Neuroscience
Huihui Liu, Jiayi Liu, Meiyu Peng, Yuhui Li, Chunqiao Li
OBJECTIVE: To investigate the effect of acupuncture on TGF-β1/Smads signaling pathway in the lung tissue of mice with airway remodeling. METHODS: Thirty specific pathogen-free mice were randomly divided into blank group, model group and acupuncture group ( n =10). Mouse models of asthma were established in the model group and the acupuncture group, and the mice in the latter group received 7 acupuncture therapies (at bilateral Fei Shu, Da Zhui and Zu Sanli, 20 min each time) every other day, starting on the 10th day after the modeling...
November 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Chung-Jung Liu, Yao-Kuang Wang, Fu-Chen Kuo, Wen-Hung Hsu, Fang-Jung Yu, Shuchen Hsieh, Ming-Hong Tai, Deng-Chyang Wu, Chao-Hung Kuo
Hepatoma-derived growth factor (HDGF) plays a critical role in tumor cell proliferation, anti-apoptosis, VEGF expression, lymph node metastasis and poor prognosis in human gastric cancer. Gastric cancer, as one of the most prevalent cancers worldwide, is the second leading cause of cancer-related mortality in the world for the prognosis of gastric cancer is generally poor, especially in patients with advanced stage. Helicobacter pylori ( H. pylori ) infection causes the chronic inflammation of stomach as well as the development of gastric cancer, with a three to six-fold increased risk of gastric cancer...
November 30, 2018: Cancers
Beibing Di, Hong-Wei Li, Weiping Li, Bing Hua
Vascular smooth muscle cell (VSMC) phenotype transition is involved in diabetes-associated cardiovascular diseases. The mechanism of VSMCs phenotypic transition in T2DM was still unclear. Rat coronary artery SMCs were pretreated with liraglutide alone, liraglutide and H89(a PKA inhibitor), neutralizing anti-RAGE antibody or the antioxidant pyrrolidine dithiocarbamate (PDTC; a nuclear factor-κB (NF-κB) inhibitor), followed by treatment with AGE. The morphological change of the SMCs was observed. We also observed the α-actin positive myofilaments and F-actin distribution in SMC through immunofluorescence microscopy...
December 1, 2018: Peptides
Adrian H Kopf, Martijn C Koorengevel, Cornelis A van Walree, Timothy R Dafforn, J Antoinette Killian
Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave...
December 1, 2018: Chemistry and Physics of Lipids
Jun Tong, Fang Chen, Wei Du, Jun Zhu, Zhenggao Xie
BACKGROUND: To determine the involvement of MicroRNA-200b (miR-200b) in human Tenon's fibroblasts (HTFs) fibrosis. METHODS: HTFs were treated with 10 ng/ml TGF-β1 for 24 hours. Expression of α-smooth muscle actin (α-SMA), Fibronectin and Collagen1A1 (COL1A1) was assessed by western blot and immunofluorescence. MiR-200b was detected by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), and its potential target genes were validated by luciferase assay...
December 4, 2018: Current Eye Research
Christopher Tsao, Laura Pandolfi, Xin Wang, Silvia Minardi, Cristina Lupo, Michael Evangelopoulos, Troy Hendrickson, Aaron Shi, Gianluca Storci, Francesca Taraballi, Ennio Tasciotti
The use of nanomaterials as carriers for the delivery of growth factors has been applied to a multitude of applications in tissue engineering. However, issues of toxicity, stability and systemic effects of these platforms have yet to be fully understood, especially for cardiovascular applications. Here, we proposed a delivery system composed of poly(DL-lactide-co-glycolide) acid (PLGA) and porous silica nanoparticles (pSi) to deliver vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF)...
December 4, 2018: ACS Applied Materials & Interfaces
Jing Liu, Yibo Qin, Yifan Wu, Zhiting Sun, Binhan Li, Huimin Jing, Chuangnian Zhang, Chen Li, Xigang Leng, Zhihong Wang, Deling Kong
Small diameter vascular grafts have been promising substitutes for bypass surgery to treat cardiovascular disease. However, no ideal product is available in the clinic. In order to design improved, next generation vascular grafts, it is essential to understand the cellular and molecular mechanisms underlying tissue regeneration after vascular graft implantation. Two diverse microenvironments, circulating blood and the surrounding tissue, are involved in the regeneration process after vascular graft implantation in situ...
December 4, 2018: Biomaterials Science
Samuel Eneọjọ Abah, Florence Burté, Sandrine Marquet, Biobele J Brown, Francis Akinkunmi, Gbeminiyi Oyinloye, Nathaniel K Afolabi, Samuel Omokhodion, Ikeoluwa Lagunju, Wuraola A Shokunbi, Mats Wahlgren, Hélia Dessein, Laurent Argiro, Alain J Dessein, Boris Noyvert, Lilian Hunt, Greg Elgar, Olugbemiro Sodeinde, Anthony A Holder, Delmiro Fernandez-Reyes
Severe Malarial Anemia (SMA), a life-threatening childhood Plasmodium falciparum malaria syndrome requiring urgent blood transfusion, exhibits inflammatory and hemolytic pathology. Differentiating between hypo-haptoglobinemia due to hemolysis or that of genetic origin is key to understand SMA pathogenesis. We hypothesized that while malaria-induced hypo-haptoglobinemia should reverse at recovery, that of genetic etiology should not. We carried-out a case-control study of children living under hyper-endemic holoendemic malaria burden in the sub-Saharan metropolis of Ibadan, Nigeria...
December 3, 2018: Scientific Reports
Lies Moonen, Hilde Geryl, Patrick C D'Haese, Benjamin A Vervaet
BACKGROUND: Acute kidney injury (AKI) is an underestimated, yet important, risk factor for the development of chronic kidney disease (CKD). Persistence of inflammation after a renal ischemic injury has been observed, both in experimental models and patients, and is thought to be an important mechanisms underlying progression of acute-to-chronic renal injury. Temporary suppression of inflammation immediately after AKI might therefore be a good first-line therapeutic strategy towards a better long term outcome...
December 3, 2018: BMC Nephrology
Julius E Kieswich, Jianmin Chen, Samira Alliouachene, Paul W Caton, Kieran McCafferty, Christoph Thiemermann, Muhammad M Yaqoob
BACKGROUND: The end stage renal disease population has a 20 fold higher incidence of cardiovascular mortality compared to the overall population. The development of reno-cardiac syndrome in these patients will result in cardiovascular events to be the cause of 50% of fatalities. There is therefore a need to research improved therapeutic strategies to combat renal cardiac pathologies. Murine in vivo models contribute greatly to such research allowing for specific genetic modification and reduced miscellany, however there is currently no reliable model of reno-cardiac syndrome in the most common genetically modified mouse strain, the C57BL/6...
December 4, 2018: BMC Nephrology
Sarah Ahmed Mohamed Mahmoud, Hatem Wael Amer, Sally Ibrahim Mohamed
Ameloblastic carcinoma (AC) is an extremely rare malignant odontogenic tumour arising from odontogenic epithelium. It was classified into primary type and secondary type. A previous study revealed that primary ameloblastic carcinoma cases were associated with more favourable prognosis than secondary cases. The aim of the present work was: to report the clinical, histopathological, immunohistochemical, and ploidy status, and therapeutic details of four cases of primary AC, and to review the literature with regard to clinical, follow-up, prognosis, histopathological, and immunohistochemical information of primary AC...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
Michael Overduin, Mansoore Esmaili
The concept of a memtein as the minimal unit of membrane function is proposed here, and refers to the complex of a membrane protein together with a continuous layer of biological lipid molecules. The elucidation of the atomic resolution structures and specific interactions within memteins remains technically challenging. Nonetheless, we argue that these entities are critical endpoints for the postgenomic era, being essential units of cellular function that mediate signal transduction and trafficking. Their biological mechanisms and molecular compositions can be resolved using native nanodiscs formed by poly(styrene-co-maleic acid) (SMA) copolymers...
November 30, 2018: Chemistry and Physics of Lipids
Aijun Liu, Yifan Liu, Bin Li, Ming Yang, Yang Liu, Junwu Su
OBJECTIVES: To investigate the functions of miR-223-3p and ITGB3 in pulmonary arterial hypertension (PAH). MATERIALS AND METHODS: Microarray analysis was used to detect differentially expressed genes and microRNAs. In in vitro models, the expressions of miR-223-3p and ITGB3 were detected by qRT-PCR and Western blot. α-SMA expression and cell proliferation were analysed by immunofluorescence and MTT assay, respectively. In in vivo models, PAH progressions were determined by measuring the levels of mPAP and RVSP...
December 3, 2018: Cell Proliferation
Xiangyu Wang, Chao Xu, Linhao Chen, Liangxiang Huang, Changqing Zeng, Fangqin Xue, Yu Zheng, Liangjie Chi, Dajia Lin, Lifeng Xie
OBJECTIVE: To investigate the safety of the controllable ileostomy with pipe in view of histology. METHODS: Twenty-eight Beagle dogs undergoing controllable ileostomy with pipe were studied. The special fistula tube with balloon was placed into the hole locating at the cecal root opposing the mesenteric side, and fixed by double knot compression method. RESULTS: The fistula tube was removed 14 days after surgery, then the safety of the procedure was preliminarily evaluated by gastrointestinal radiography and anatomical observation...
November 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
Houman Alimoradi, Anita Barzegar-Fallah, Ivan A Sammut, Khaled Greish, Gregory I Giles
Nitric oxide (NO) donor drugs have a range of clinical applications, and are also being developed as therapeutics for the potential treatment of multiple diseases. This article presents data describing the synthesis and characterisation of a novel NO releasing nanoparticle formed by encapsulation of the NO donor tDodSNO into a co-polymer of styrene and maleic acid (SMA) to afford SMA-tDodSNO. The pharmacological activity of SMA-tDodSNO is discussed in our accompanying manuscript "Encapsulation of tDodSNO generates a photoactivated nitric oxide releasing nanoparticle for localized control of vasodilation and vascular hyperpermeability"...
December 2018: Data in Brief
Seo-Hyun Choi, A-Ram Kim, Jae-Kyung Nam, Jin-Mo Kim, Jee-Youn Kim, Haeng Ran Seo, Hae-June Lee, Jaeho Cho, Yoon-Jin Lee
It remains controversial whether targeting tumour vasculature can improve radiotherapeutic efficacy. We report that radiation-induced endothelial-to-mesenchymal transition (EndMT) leads to tumour vasculature with abnormal SMA+ NG2+ pericyte recruitment during tumour regrowth after radiotherapy. Trp53 (but not Tgfbr2) deletion in endothelial cells (ECs) inhibited radiation-induced EndMT, reducing tumour regrowth and metastases with a high CD44v6+ cancer-stem-cell (CSC) content after radiotherapy. Osteopontin, an EndMT-related angiocrine factor suppressed by EC-Trp53 deletion, stimulated proliferation in dormant CD44v6+ cells in severely hypoxic regions after radiation...
November 30, 2018: Nature Communications
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