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Schistosoma mansoni

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https://www.readbyqxmd.com/read/28431921/optimization-of-the-helmintex-method-for-schistosomiasis-diagnosis
#1
Vivian Favero, Renata Russo Frasca Candido, Carolina De Marco Verissimo, Malcolm K Jones, Timothy G St Pierre, Catieli Gobetti Lindholz, Vinicius Duval Da Silva, Alessandra Loureiro Morassutti, Carlos Graeff-Teixeira
A diagnostic test that is reliable, sensitive, and applicable in the field is extremely important in epidemiological surveys, during medical treatment for schistosomiasis, and for the control and elimination of schistosomiasis. The Helmintex (HTX) method is based on the use of magnetic beads to trap eggs in a magnetic field. This technique is highly sensitive, but the screening of fecal samples consumes lots of time, thus delaying the results, especially in field studies. The objective of this work was to determine the effects of incorporation of the detergent Tween-20 into the method in an attempt to decrease the final pellet volume produced by the HTX method as well as the use of ninhydrin to stain the Schistosoma mansoni eggs...
April 19, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28430889/accuracy-of-diagnostic-tests-for-schistosoma-mansoni-infection-in-asymptomatic-eritrean-refugees-serology-and-poc-cca-against-stool-microscopy
#2
Afona Chernet, Kerstin Kling, Véronique Sydow, Esther Kuenzli, Christoph Hatz, Jürg Utzinger, Lisette van Lieshout, Hanspeter Marti, Niklaus D Labhardt, Andreas Neumayr
Background: The unprecedented increase in number of African refugees arriving in Europe is confronting clinicians and general practitioners with the question of whether or not and how to screen migrants from endemic regions for Schistosoma mansoni infection. Methods: We assessed the accuracy of three different diagnostic tests for S.mansoni infection (stool microscopy [samples prepared by sedimentation technique], serology, and point-of-care circulating cathodic antigen [POC-CCA] urine cassette test) in 107 newly arrived asymptomatic Eritrean refugees in Switzerland...
April 19, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28408356/possible-antifibrotic-effect-of-gdc-0449-vismodegib-a-hedgehog-pathway-inhibitor-in-mice-model-of-schistosoma-induced-liver-fibrosis
#3
Abeer A Elhenawy, Rehab H Ashour, Nairmen Nabih, Naglaa M Shalaby, Nirmeen Megahed
Liver fibrosis is a pathological process complicating schistosomiasis. It is an active process of continuous extracellular matrix accumulation. In Egypt, schistosomiasis re-infection is a continuing problem especially in rural areas. In this study we examined the antifibrotic effect of GDC-0449 (Vismodegib), a hedgehog-pathway inhibitor as a new molecular target for Schistosoma-induced liver fibrosis, in addition to exploring its effect as antischistosomal drug. The effect of GDC-0449 alone or combined with Praziquantel was tried experimentally in infected mice with Schistosoma mansoni...
April 10, 2017: Parasitology International
https://www.readbyqxmd.com/read/28407789/identification-and-functional-characterisation-of-a-schistosoma-japonicum-insulin-like-peptide
#4
Xiaofeng Du, Donald P McManus, Pengfei Cai, Wei Hu, Hong You
BACKGROUND: Previous studies have shown that insulin receptors in schistosomes, triggered by host insulin, play an important role in parasite growth, development and fecundity by regulating glucose metabolism. However, limited information is available on the recently identified endogenous insulin-like peptide (ILP) in blood flukes. RESULTS: We isolated ILPs from Schistosoma japonicum (SjILP) and S. recognised (SmILP) and present results of their molecular and structural analysis...
April 14, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28406899/histone-deacetylase-inhibition-modulates-histone-acetylation-at-gene-promoter-regions-and-affects-genome-wide-gene-transcription-in-schistosoma-mansoni
#5
Letícia Anderson, Monete Rajão Gomes, Lucas Ferreira daSilva, Adriana da Silva Andrade Pereira, Marina M Mourão, Christophe Romier, Raymond Pierce, Sergio Verjovski-Almeida
BACKGROUND: Schistosomiasis is a parasitic disease infecting hundreds of millions of people worldwide. Treatment depends on a single drug, praziquantel, which kills the Schistosoma spp parasite only at the adult stage. HDAC inhibitors (HDACi) such as Trichostatin A (TSA) induce parasite mortality in vitro (schistosomula and adult worms), however the downstream effects of histone hyperacetylation on the parasite are not known. METHODOLOGY/PRINCIPAL FINDINGS: TSA treatment of adult worms in vitro increased histone acetylation at H3K9ac and H3K14ac, which are transcription activation marks, not affecting the unrelated transcription repression mark H3K27me3...
April 13, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28393916/production-and-glyco-engineering-of-immunomodulatory-helminth-glycoproteins-in-plants
#6
Ruud H P Wilbers, Lotte B Westerhof, Kim van Noort, Katja Obieglo, Nicole N Driessen, Bart Everts, Sonja I Gringhuis, Gabriele Schramm, Aska Goverse, Geert Smant, Jaap Bakker, Hermelijn H Smits, Maria Yazdanbakhsh, Arjen Schots, Cornelis H Hokke
Helminth parasites control host-immune responses by secreting immunomodulatory glycoproteins. Clinical trials and mouse model studies have demonstrated the potential of helminth-derived glycoproteins for the treatment of immune-related diseases, like allergies and autoimmune diseases. Studies are however hampered by the limited availability of native parasite-derived proteins. Moreover, recombinant protein production systems have thus far been unable to reconstitute helminth-like glycosylation essential for the functionality of some helminth glycoproteins...
April 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28392476/schistosoma-mansoni-displays-an-adenine-phosphoribosyltransferase-preferentially-expressed-in-mature-female-gonads-and-vitelaria
#7
Ana Eliza Zeraik, Vitor Hugo Balasco Serrão, Larissa Romanello, Juliana Roberta Torini, Alexandre Cassago, Ricardo DeMarco, Humberto D'Muniz Pereira
Schistosoma mansoni depends upon the purine salvage pathway to obtain purine nucleotides; therefore, enzymes from this pathway are essential for parasite survival. Here, we focused on the adenine phosphoribosyltransferase (APRT) enzyme, which catalyzes the condensation reaction between adenine and PRPP (5-phosphoribosylpyrophosphate) to produce AMP and PPi. Kinetic experiments using the heterologously expressed protein of one APRT isoform from S. mansoni indicate that it is catalytically active, and whole-mount in situ hybridization studies indicate that the transcripts of this protein are concentrated in the posterior region of the ovary and vitellaria of female adult worms...
April 6, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28389350/process-development-for-production-and-purification-of-the-schistosoma-mansoni-sm14-antigen
#8
Leonardo Damasceno, Gerd Ritter, Carl A Batt
The trematode Schistosoma mansoni Sm14 antigen was expressed in the yeast Pichia pastoris and secreted into the culture medium at yields of approximately 250 mg L(-1). Sm14 belongs to a family of fatty-acid binding proteins and appears to play an important role in uptake, transport, and compartmentalization of lipids in S. mansoni and it is a potential vaccine candidate in both humans and domesticated animals. The Sm14 gene was codon-optimized for expression in P. pastoris, and placed under transcription of the strong methanol inducible AOX1 promoter...
April 5, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28380119/synthesis-and-biological-evaluation-of-novel-imidazolidine-derivatives-as-candidates-to-schistosomicidal-agents
#9
Thiago José Matos-Rocha, Maria do Carmo Alves de Lima, Anekécia Lauro da Silva, Jamerson Ferreira de Oliveira, Allana Lemos Andrade Gouveia, Vinícius Barros Ribeiro da Silva, Antônio Sérgio Alves de Almeida, Fábio André Brayner, Pablo Ramon Gualberto Cardoso, Marina da Rocha Pitta-Galdino, Ivan da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Luiz Carlos Alves, Maira Galdino da Rocha Pitta
Introduction: Schistosomiasis is an infectious parasitic disease caused by trematodes of the genus Schistosoma, which threatens at least 258 million people worldwide and its control is dependent on a single drug, praziquantel. The aim of this study was to evaluate the anti-Schistosoma mansoni activity in vitro of novel imidazolidine derivatives. Material and methods: We synthesized two novel imidazolidine derivatives: (LPSF/PTS10) (Z)-1-(2-chloro-6-fluorobenzyl)-4-(4-dimethylaminobenzylidene)-5-thioxoimidazolidin-2-one and (LPSF/PTS23) (Z)-1-(2-chloro-6-fluoro-benzyl)-5-thioxo-4-(2,4,6-trimethoxy-benzylidene)-imidazolidin-2-one...
April 3, 2017: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/28373144/efficacy-of-soluble-glycoprotein-fraction-from-allium-sativum-purified-by-size-exclusion-chromatography-on-murine-schistosomiasis-mansoni
#10
Ibrahim Aly, Eman E Taher, Hoda El-Sayed, Faten A Mohammed, Gehan ELnain, Rabab S Hamad, Elsayed M Bayoumy
In this work, the efficiency of crude MeOH extracts and soluble glycoprotein fraction of Allium sativum purified by size-exclusion chromatography (SEC) on parasitological, histopathological and some biochemical parameters in Schistosoma mansoni infected mice were investigated. Animals were infected by tail immersion with 100 cercariae/each mouse and divided into five groups in addition to the normal control. The results revealed a significant decrease in mean worm burden in all treated mice especially in the group treated with soluble glycoprotein fraction of A...
March 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28371522/epitopes-rationally-selected-through-computational-analyses-induce-t-cell-proliferation-in-mice-and-are-recognized-by-serum-from-individuals-infected-with-schistosoma-mansoni
#11
Marcelo D Lopes, Flávio M Oliveira, Ivan E V Coelho, Maria J F Passos, Clarice C Alves, Alex G Taranto, Moacyr C Júnior, Luciana L Santos, Cristina T Fonseca, José A F P Villar, Débora O Lopes
Schistosomiasis is the second leading cause of death due to parasitic diseases in the world. Seeking an alternative for the control of disease, the World Health Organization funded the genome sequencing of the major species related to schistosomiasis to identify potential vaccines and therapeutic targets. Therefore, the aim of this work was to select T and B-cell epitopes from Schistosoma mansoni through computational analyses and evaluate the immunological potential of epitopes in vitro. Extracellular regions of membrane proteins from the Schistosoma mansoni were used to predict promiscuous epitopes with affinity to different human Major Histocompatibility Class II (MHCII) molecules by bioinformatics analysis...
April 3, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28369056/pulmonary-shunts-in-severe-hepatosplenic-schistosomiasis-diagnosis-by-contrast-echocardiography-and-their-relationship-with-abdominal-ultrasound-findings
#12
Liana Gonçalves-Macedo, Ana Lucia Coutinho Domingues, Edmundo Pessoa Lopes, Carlos Feitosa Luna, Vitor Gomes Mota, Mônica Moraes de Chaves Becker, Brivaldo Markman-Filho
BACKGROUND: Schistosomiasis is endemic to several parts of the world. Among the species that affect humans, Schistosoma mansoni is one of the most common causes of illness. In regions where schistosomiasis mansoni is endemic, reinfection is responsible for the emergence of hepatosplenic schistosomiasis (HSS) with portal hypertension in about 10% of infected individuals. Regardless of its etiology, portal hypertension may bring about the formation of arteriovenous fistulas and pulmonary vascular dilation, thus constituting a pulmonary shunt and its presence has been associated with the occurrence of neurological complications...
April 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28366172/the-effects-of-phosphanegold-i-thiolates-on-the-biological-properties-of-acanthamoeba-castellanii-belonging-to-the-t4-genotype
#13
Ruqaiyyah Siddiqui, Farhat Abjani, Chien Ing Yeo, Edward R T Tiekink, Naveed Ahmed Khan
BACKGROUND: Gold compounds have shown promise in the treatment of non-communicable diseases such as rheumatoid arthritis and cancer, and are considered of value as anti-microbial agents against Gram-negative and Gram-positive bacteria, and have anti-parasitic properties against Schistosoma mansoni, Trypanosoma brucei, Plasmodium falciparum, Leishmania infantinum, Giardia lamblia, and Entamoeba histolytica. They are known to affect enzymatic activities that are required for the cellular respiration processes...
April 3, 2017: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/28363777/dual-genetic-absence-of-stat6-and-il-10-does-not-abrogate-anti-hyperglycemic-effects-of-schistosoma-mansoni-in-streptozotocin-treated-diabetic-mice
#14
Yoshio Osada, Tomohiro Fujiyama, Naoto Kamimura, Tsukushi Kaji, Susumu Nakae, Katsuko Sudo, Kenji Ishiwata, Tamotsu Kanazawa
Schistosoma mansoni (Sm) is known to exert protective effects against various allergic and autoimmune disorders. It has been reported that this parasite protects NOD mice from spontaneous type 1 diabetes (T1D) and ameliorates streptozotocin (STZ)-induced T1D in wild-type mice. Here, we tried to clarify the anti-diabetic mechanisms of Sm in the latter model. Sm infection partially prevented the degradation of pancreatic islets and hyperglycemia in multiple low-dose (MLD) STZ-treated mice. Neither Treg cell depletion nor genetic absences of IL-10 and/or STAT6 abrogated the anti-hyperglycemic effects of Sm...
March 28, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28363230/ovarian-manson-s-schistosomiasis-rare-diagnosis-or-underestimated-prevalence
#15
Elainne Christine de Souza Gomes, Ana Lúcia Coutinho Domingues, Francisco Carlos Amanajás de Aguiar Júnior, Constança Simões Barbosa
The occurrence of Manson's schistosomiasis in organs of the female reproductive tract is an uncommon event, given that the etiological agent for this disease is a blood parasite that inhabits the mesenteric veins. In this case report, a 45-year-old female patient reported that her first symptoms had been strong pain in the left iliac region around two years earlier. An endovaginal pelvic ultrasonography showed that the left ovary was enlarged, and the report suggested that this finding might be correlated with clinical data and tumor markers...
March 31, 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28362351/barbatic-acid-offers-a-new-possibility-for-control-of-biomphalaria-glabrata-and-schistosomiasis
#16
Mônica Cristina Barroso Martins, Monique Costa Silva, Hianna Arely Milca Fagundes Silva, Luanna Ribeiro Santos Silva, Mônica Camelo Pessoa de Azevedo Albuquerque, André Lima Aires, Emerson Peter da Silva Falcão, Eugênia C Pereira, Ana Maria Mendonça Albuquerque de Melo, Nicácio Henrique da Silva
This study evaluated the biological activity of an ether extract and barbatic acid (BAR) from Cladia aggregata on embryos and adult mollusks of Biomphalaria glabrata, cercariae of Schistosoma mansoni and the microcrustacean Artemia salina. The ether extract and BAR were obtained by successive extractions with diethyl ether. The obtained extracts were analyzed using thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), proton nuclear magnetic resonance (¹H-NMR) and infrared (IR) spectroscopy...
March 31, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28355401/schistosoma-mansoni-associated-glomerulopathy-with-iga-mesangial-deposits-case-report
#17
Fabiana Oliveira Gonçalves, Tânia Maria de Souza Fontes, Ana Paula Pereira Santana Lemes Canuto
INTRODUCTION: Renal involvement is a severe form of schistosomiasis and occurs in 10% to 15% of patients with the hepatosplenic form of the disease. Nephrotic syndrome is the most common clinical presentation. It is a complication caused by immune complexes (IC), it is rare to appear in the Brazilian context with a immunoglobulin A (IgA) deposits. When installed the renal injury by Schistosoma mansoni, classically presents as membranoproliferative glomerulonephritis (mesangiocapillary) with lobular accentuation...
March 2017: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
https://www.readbyqxmd.com/read/28348881/in-vitro-assessment-of-anthelmintic-activities-of-rauwolfia-vomitoria-apocynaceae-stem-bark-and-roots-against-parasitic-stages-of-schistosoma-mansoni-and-cytotoxic-study
#18
Emmanuel Mouafo Tekwu, Kwabena Mante Bosompem, William Kofi Anyan, Regina Appiah-Opong, Kofi Baffour-Awuah Owusu, Mabel Deladem Tettey, Felicia Amanfo Kissi, Alfred Ampomah Appiah, Veronique Penlap Beng, Alexander Kwadwo Nyarko
Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62...
2017: Journal of Parasitology Research
https://www.readbyqxmd.com/read/28347672/structural-and-kinetic-analysis-of-schistosoma-mansoni-adenylosuccinate-lyase-smadsl
#19
Larissa Romanello, Vitor Hugo Balasco Serrão, Juliana Roberta Torini, Louise E Bird, Joanne E Nettleship, Heather Rada, Yamini Reddivari, Ray J Owens, Ricardo DeMarco, José Brandão-Neto, Humberto D'Muniz Pereira
Schistosoma mansoni is the parasite responsible for schistosomiasis, a disease that affects about 218 million people worldwide. Currently, both direct treatment and disease control initiatives rely on chemotherapy using a single drug, praziquantel. Concerns over the possibility of resistance developing to praziquantel, have stimulated efforts to develop new drugs for the treatment of schistosomiasis. Schistosomes do not have the de novo purine biosynthetic pathway, and instead depend entirely on the purine salvage pathway to supply its need for purines...
March 25, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28346957/-a-young-asylum-seeker-with-hemoptysis-and-positive-tuberculosis-screening-not-always-tuberculosis
#20
T Stevens, S B Schwarz, F S Magnet, W Windisch
This paper reports on the case of a 19 year old asylum seeker from Eritrea who presented with hemoptysis, a positive tuberculosis screening (Enzyme Linked Immuno Spot Assay - EliSpot) and mushy faeces submitted with a suspected diagnosis of tuberculosis. Laboratory testing revealed thrombopenia, leukopenia and eosinophilia, while the chest X-ray was inconspicuous. Acid-proof rod bacteria were neither evident in bronchoscopy samples nor in expectorated sputum samples. However, sonographic findings showed a profound splenomegaly, and laboratory testing revealed a Schistosoma mansoni infection...
March 27, 2017: Pneumologie
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