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https://www.readbyqxmd.com/read/30117167/the-regulators-of-g-protein-signaling-rgs16-and-rgs18-inhibit-protease-activated-receptor-2-gi-o-signaling-through-distinct-interactions-with-g%C3%AE-in-live-cells
#1
Kiman Kim, Jinyong Lee, Sungho Ghil
Protease-activated receptor 2 (PAR2) is a G protein-coupled receptor (GPCR) activated by endogenous proteases, in particular, trypsin. Although regulators of G protein signaling (RGS) are known to inhibit GPCR/Gα-mediated signaling, their specific effects on PAR2 are poorly understood at present. Here, we use a bioluminescence resonance energy transfer (BRET) technique to investigate whether RGS16 and RGS18 bind PAR2 in live cells to regulate PAR2/Gαi/o -mediated signaling. Notably, we find that RGS16 binds to PAR2 in the presence of Gαi while RGS18 does not interact with PAR2, regardless of the presence of Gα...
August 16, 2018: FEBS Letters
https://www.readbyqxmd.com/read/30116602/development-and-application-of-bioluminescence-imaging-for-the-influenza-a-virus
#2
REVIEW
Weiqi Pan, Ji Dong, Peihai Chen, Beiwu Zhang, Zhixia Li, Ling Chen
Influenza A viruses (IAVs) cause seasonal epidemics and intermittent pandemics which threaten human health. Conventional assays cannot meet the demands for rapid and sensitive detection of viral spread and pathogenesis in real time cannot be used for high-throughput screens of novel antivirals. Bioluminescence imaging (BLI) has emerged as a powerful tool in the study of infectious diseases in animal models. The advent of influenza reverse genetics has enabled the incorporation of bioluminescent reporter proteins into replication-competent IAVs...
July 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/30116040/genetic-diversity-and-phenotypic-plasticity-of-ahl-mediated-quorum-sensing-in-environmental-strains-of-vibrio-mediterranei
#3
Léa Girard, François Lantoine, Raphaël Lami, Florence Vouvé, Marcelino T Suzuki, Julia Baudart
N-Acyl homoserine lactone (AHL)-mediated Quorum sensing (QS) is one of the most studied social behavior among Proteobacteria. However, despite the current knowledge on QS-associated phenotypes such as bioluminescence, biofilm formation, or pathogenesis, the characterization of environmental factors driving QS in realistic ecological settings remains scarce. We investigated the dynamics of AHL and AHL-producing Vibrio among 840 isolates  collected fortnightly from the Salses-Leucate Mediterranean lagoon in spring and summer 2015 and 2016...
August 16, 2018: ISME Journal
https://www.readbyqxmd.com/read/30112660/a-luciferase-complementation-assay-system-using-transferable-mouse-artificial-chromosomes-to-monitor-protein-protein-interactions-mediated-by-g-protein-coupled-receptors
#4
Narumi Uno, Tomohito Fujimoto, Shinya Komoto, Gene Kurosawa, Masaaki Sawa, Teruhiko Suzuki, Yasuhiro Kazuki, Mitsuo Oshimura
G protein-coupled receptors (GPCRs) are seven-transmembrane domain receptors that interact with the β-arrestin family, particularly β-arrestin 1 (ARRB1). GPCRs interact with 33% of small molecule drugs. Ligand screening is promising for drug discovery concerning GPCR-related diseases. Luciferase complementation assay (LCA) enables detection of protein-protein complementation via bioluminescence following complementation of N- and C-terminal luciferase fragments (NEluc and CEluc) fused to target proteins, but it is necessary to co-express the two genes...
July 31, 2018: Cytotechnology
https://www.readbyqxmd.com/read/30112352/development-of-dual-reporter-imaging-system-for-francisella-tularensis-to-monitor-the-spatio-temporal-pathogenesis-and-vaccine-efficacy
#5
Young-Hwa Kim, Pil-Gu Park, Sang-Hwan Seo, Kee-Jong Hong, Hyewon Youn
Purpose: Study on the pathogen and the pathogen-related disease require the information at both cellular and organism level. However, lack of appropriate high-quality antibodies and the difference between the experimental animal models make it difficult to analyze in vivo mechanism of pathogen-related diseases. For more reliable research on the infection and immune-response of pathogen-related diseases, accurate analysis is essential to provide spatiotemporal information of pathogens and immune activity to avoid false-positive or mis-interpretations...
July 2018: Clinical and Experimental Vaccine Research
https://www.readbyqxmd.com/read/30109785/bioluminescence-and-chemiluminescence-abilities-of-trans-3-hydroxyhispidin-on-the-luminous-fungus-mycena-chlorophos
#6
Katsunori Teranishi
The fungus Mycena chlorophos emits green light from its pileus gills but not from its stipes. The chemical mechanisms underlying its bioluminescence are unclear. Trans-3-hydroxyhispidin has been known to be a luminescence substrate for the bioluminescent mycelia of Neonothopanus nambi and N. gardneri. In the present study, the bioluminescence and chemiluminescence abilities of trans-3-hydroxyhispidin on pileus gills and originally non-bioluminescent stipes of M. chlorophos were demonstrated. Trans-3-hydroxyhispidin induced bioluminescence of living gills and stipes...
August 15, 2018: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/30108719/development-of-highly-active-anti-pneumocystis-bisbenzamidines-insight-into-the-influence-of-selected-substituents-on-the-in-vitro-activity
#7
D Maciejewska, J Żabiński, M Rezler, P Kaźmierczak, M S Collins, L Ficker, M T Cushion
Here we describe the potency of 21 pentamidine analogues against the fungal pathogen, Pneumocystis carinii , in an ATP bioluminescent assay with toxicity profiles in 2 mammalian cell lines. Reduction of two 5-methyl-1,2,4-oxadiazole rings was applied to the synthesis of acid-labile bisamidines. Anti- Pneumocystis activity is discussed in the context of 3 groups of compounds depending on the main structural changes of the pentamidine lead structure. The groups include: 1) 1,4-bis(methylene)piperazine derivatives 1-5 ; 2) alkanediamide derivatives 6-10 ; 3) alkane-derived bisbenzamidines 11-21 ...
October 1, 2017: MedChemComm
https://www.readbyqxmd.com/read/30108159/-in-vitro-pharmacological-characterization-of-buprenorphine-samidorphan-and-combinations-being-developed-as-an-adjunctive-treatment-for-major-depressive-disorder
#8
Jean M Bidlack, Brian I Knapp, Daniel R Deaver, Margarita Plotnikava, Derrick Arnelle, Angela M Wonsey, May Fenn Toh, Sokhom S Pin, Mark N Namchuk
A combination of buprenorphine (BUP) and samidorphan (SAM) at a 1:1 fixed dose ratio (mg/mg), is being investigated as an adjunctive treatment for major depressive disorder (BUP/SAM, ALKS 5461). Both [3 H]BUP and [3 H]SAM bound to the mu, kappa and delta opioid receptors (MOR, KOR and DOR, respectively) with Kd values of 3 nM or less. [3 H]BUP dissociated slower from the MOR than [3 H]SAM did. In the [35 S]GTPγS assay, BUP was a partial agonist at the MOR, KOR and DOR. SAM was an antagonist at the MOR and a partial agonist at the KOR and DOR...
August 14, 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/30107778/real-time-monitoring-of-pseudomonas-aeruginosa-biofilm-formation-on-endotracheal-tubes-in-vitro
#9
Eva Pericolini, Bruna Colombari, Gianmarco Ferretti, Ramona Iseppi, Andrea Ardizzoni, Massimo Girardis, Arianna Sala, Samuele Peppoloni, Elisabetta Blasi
BACKGROUND: Pseudomonas aeruginosa is an opportunistic bacterial pathogen responsible for both acute and chronic infections in humans. In particular, its ability to form biofilm, on biotic and abiotic surfaces, makes it particularly resistant to host's immune defenses and current antibiotic therapies as well. Innovative antimicrobial materials, like hydrogel, silver salts or nanoparticles have been used to cover new generation catheters with promising results. Nevertheless, biofilm remains a major health problem...
August 14, 2018: BMC Microbiology
https://www.readbyqxmd.com/read/30101620/evaluating-non-integrating-lentiviruses-as-safe-vectors-for-noninvasive-reporter-based-molecular-imaging-of-multipotent-mesenchymal-stem-cells
#10
Amanda Hamilton, Paula J Foster, John A Ronald
Reporter gene-based molecular imaging can provide invaluable information on the fate of cellular therapies post-implantation. Integrating lentiviral vectors (ILVs) are commonly used for stably engineering cells, however, their potential for insertional mutagenesis poses a significant safety concern and barrier to widespread clinical adoption. In cells that slowly divide or are post-mitotic in vivo, such as mesenchymal stem cells (MSCs), non-integrating lentiviral vectors (NILVs) may be a safer alternative option since NILVs remain episomal and can provide prolonged expression profiles...
August 13, 2018: Human Gene Therapy
https://www.readbyqxmd.com/read/30101537/neuraminidase-mediated-desialylation-augments-aav9-mediated-gene-expression-in-skeletal-muscle
#11
Hongling Zhu, Tao Wang, R John Lye, Brent A French, Brian H Annex
BACKGROUND: Following systemic delivery, AAV9-mediated gene expression is significantly increased in ischemic versus non-ischemic muscle, suggesting AAV9 as an attractive vector for treating peripheral arterial disease (PAD). Potential mechanisms underlying ischemia-augmented expression include: 1) increased vascular permeability, and 2) "unmasking" of endogenous AAV9 receptors. Here, we endeavored to reconstitute the ischemic induction of AAV9 in vivo, using local injection of histamine (to increase vascular permeability) and neuraminidase (to desialylate cell surface glycans)...
August 12, 2018: Journal of Gene Medicine
https://www.readbyqxmd.com/read/30100954/student-centered-microbioassay-laboratory-activity-utilizing-bioluminescent-bacteria
#12
Kerri Younkin, Christina Romano
Student-centered teaching allows students to be actively engaged in hands-on, minds-on activities that emphasize creativity and collaboration, enabling them to ask questions and design their own investigations to real-world problems. One such problem is water contamination, which causes human health and environmental issues. However, chemical water quality testing for pollutants can be timely and expensive. In addition to chemical testing, researchers have developed assays using unicellular organisms to determine which pollutants are present and in what concentrations...
2018: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/30098135/high-performance-robustly-verified-monte-carlo-simulation-with-fullmonte
#13
Jeffrey Cassidy, Ali Nouri, Vaughn Betz, Lothar Lilge
We introduce the FullMonte tetrahedral 3-D Monte Carlo (MC) software package for simulation, visualization, and analysis of light propagation in heterogeneous turbid media including tissue. It provides the highest computational performance and richest set of input, output, and analysis facilities of any open-source tetrahedral-mesh MC light simulator. It also provides a robust framework for statistical verification. A scripting interface makes set-up of simulation runs simple, including parameter sweeps, while simultaneously providing customization options...
August 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/30093406/14-3-3%C3%AE-binds-rgs14-at-distinct-sites-to-inhibit-the-rgs14-g%C3%AE-i-alf-4-signaling-complex-and-rgs14-nuclear-localization
#14
Kyle J Gerber, Katherine E Squires, John R Hepler
Regulator of G protein signaling 14 (RGS14) is a multifunctional brain scaffolding protein that integrates G protein and Ras/ERK signaling pathways. It is also a nucleo-cytoplasmic shuttling protein. RGS14 binds active Gαi/o via its RGS domain, Raf and active H-Ras-GTP via its R1 Ras binding domain (RBD), and inactive Gαi1/3 via its G protein regulatory (GPR) domain. RGS14 suppresses long term potentiation (LTP) in the CA2 region of the hippocampus, thereby regulating hippocampal-based learning and memory...
August 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30092403/improved-lentiviral-gene-delivery-to-mouse-liver-by-hydrodynamic-vector-injection-through-tail-vein
#15
Trine Dalsgaard, Claudia R Cecchi, Anne Louise Askou, Rasmus O Bak, Pernille O Andersen, David Hougaard, Thomas G Jensen, Frederik Dagnæs-Hansen, Jacob Giehm Mikkelsen, Thomas J Corydon, Lars Aagaard
Delivery of genes to mouse liver is routinely accomplished by tail-vein injections of viral vectors or naked plasmid DNA. While viral vectors are typically injected in a low-pressure and -volume fashion, uptake of naked plasmid DNA to hepatocytes is facilitated by high pressure and volumes, also known as hydrodynamic delivery. In this study, we compare the efficacy and specificity of delivery of vesicular stomatitis virus G glycoprotein (VSV-G) pseudotyped lentiviral vectors to mouse liver by a number of injection schemes...
August 6, 2018: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/30092165/novel-alternatives-to-extracellular-vesicle-based-immunotherapy-exosome-mimetics-derived-from-natural-killer-cells
#16
Liya Zhu, Prakash Gangadaran, Senthilkumar Kalimuthu, Ji Min Oh, Se Hwan Baek, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
Exosomes are endogenous nanocarriers that can deliver biological information between cells. They are secreted by all cell types, including immune cells such as natural killer (NK) cells. However, mammalian cells release low quantities of exosomes, and the purification of exosomes is difficult. Here, nanovesicles were developed by extrusion of NK cells through filters with progressively smaller pore sizes to obtain exosome mimetics (NK-EM). The anti-tumour effect of the NK-EM was confirmed in vitro and in vivo...
August 9, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/30091546/-biological-ultra-weak-luminescence-and-its-application-to-research-of-acupuncture
#17
Le Guo, Yi Guo, Guo-Xia Zhang, Xue-Wei Zhao, Zhi-Jun Fan, Mu-Jun Zhi, Tie Li, Fu-Chun Wang
Ultra-weak bioluminescence (UWL) is a physiological phenomenon widely existing in all the biological activities including human, animals, plants, etc., which reflects the energy metabolism of the organism. Since the last century, ultra-weak photon emission (UPE) has been applied to the study of the essence of meridians and acupoints of traditional Chinese medicine and obtained some results as the higher luminescence characteristics, but many problems remain unsolved due to the limitation of detection technology...
June 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/30089107/the-transcriptome-of-the-bermuda-fireworm-odontosyllis-enopla-annelida-syllidae-a-unique-luciferase-gene-family-and-putative-epitoky-related-genes
#18
Mercer R Brugler, M Teresa Aguado, Michael Tessler, Mark E Siddall
The Bermuda fireworm Odontosyllis enopla exhibits an extremely tight circalunar circadian behavior that results in an impressive bioluminescent mating swarm, thought to be due to a conventional luciferase-mediated oxidation of a light-emitting luciferin. In addition, the four eyes become hypertrophied and heavily pigmented, and the nephridial system is modified to store and release gametes and associated secretions. In an effort to elucidate transcripts related to bioluminescence, circadian or circalunar periodicity, as well as epitoky-related changes of the eyes and nephridial system, we examined the transcriptomic profile of three female O...
2018: PloS One
https://www.readbyqxmd.com/read/30086893/bioluminescent-snp-genotyping-technique-development-and-application-for-detection-of-melanocortin-1-receptor-gene-polymorphisms
#19
Eugenia E Bashmakova, Vasilisa V Krasitskaya, Alexander A Bondar, Ekaterina N Eremina, Eugene V Slepov, Ruslan A Zukov, Ludmila A Frank
SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants' frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals...
November 1, 2018: Talanta
https://www.readbyqxmd.com/read/30085663/the-retinitis-pigmentosa-linked-mutations-in-transmembrane-helix-5-of-rhodopsin-disrupt-cellular-trafficking-independently-of-oligomerization-state
#20
Paul Mallory, Elizabeth Gutierrez, Margaret Pinkevitch, Christie Klinginsmith, William D Comar, Francis Roushar, Adam W Smith, Beata Jastrzebska, Jonathan Schlebach
G protein-coupled receptors (GPCRs) can exist as dimers and higher oligomers in biological membranes. The specific oligomeric assembly of these receptors is believed to play a major role in their function, and the disruption of native oligomers has been implicated in specific human pathologies. Computational predictions and biochemical analyses suggest that two molecules of rhodopsin (Rho) associate through the interactions involving its fifth transmembrane helix (TM5). Interestingly, there are several pathogenic loss-of-function mutations within TM5 that face the lipid bilayer in a manner that could potentially influence the dimerization of Rho...
August 7, 2018: Biochemistry
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