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https://www.readbyqxmd.com/read/30552393/development-of-a-highly-sensitive-enzyme-linked-immunosorbent-assay-elisa-through-use-of-poly-protein-g-expressing-cell-based-microplates
#1
Yi-Jou Chen, Michael Chen, Yuan-Chin Hsieh, Yu-Cheng Su, Chang-Hung Wang, Chiu-Min Cheng, An-Pei Kao, Kai-Hung Wang, Jing-Jy Cheng, Kuo-Hsiang Chuang
The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells on microplates to improve the coating amount and displayed orientation of capture antibodies. One or eight repeated fragment crystallisable (Fc) binding domains of protein G are stably expressed on the surface of BALB/c 3T3 cells (termed 1pG cells or 8pG cells), which then act as highly antibody-trapping microparticles...
December 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30551111/application-of-an-emulsified-polycolloid-substrate-biobarrier-to-remediate-petroleum-hydrocarbon-contaminated-groundwater
#2
T H Lee, D C W Tsang, W H Chen, F Verpoort, Y T Sheu, C M Kao
Emulsified polycolloid substrate (EPS) was developed and applied in situ to form a biobarrier for the containment and enhanced bioremediation of a petroleum-hydrocarbon plume. EPS had a negative zeta potential (-35.7 mv), which promoted its even distribution after injection. Batch and column experiments were performed to evaluate the effectiveness of EPS on toluene containment and biodegradation. The EPS-to-water partition coefficient for toluene (target compound) was 943. Thus, toluene had a significant sorption affinity to EPS, which caused reduced toluene concentration in water phase in the EPS/water system...
December 5, 2018: Chemosphere
https://www.readbyqxmd.com/read/30549394/transition-to-suicide-attempt-from-recent-suicide-ideation-in-u-s-army-soldiers-results-from-the-army-study-to-assess-risk-and-resilience-in-servicemembers-army-starrs
#3
James A Naifeh, Robert J Ursano, Ronald C Kessler, Alan M Zaslavsky, Matthew K Nock, Catherine L Dempsey, Danielle Bartolanzo, Tsz Hin Hinz Ng, Pablo A Aliaga, Kelly L Zuromski, Hieu M Dinh, Carol S Fullerton, Tzu-Cheg Kao, Holly B Herberman Mash, Nancy A Sampson, Gary H Wynn, Murray B Stein
BACKGROUND: Most people with suicide ideation (SI) do not attempt suicide (SA). Understanding the transition from current/recent SI to SA is important for mental health care. Our objective was to identify characteristics that differentiate SA from 30-day SI among representative U.S. Army soldiers. METHODS: Using a unique case-control design, soldiers recently hospitalized for SA (n = 132) and representative soldiers from the same four communities (n = 10,193) were administered the same questionnaire...
December 14, 2018: Depression and Anxiety
https://www.readbyqxmd.com/read/30548993/peripheral-fat-distribution-versus-waist-circumference-in-metabolic-syndrome-for-predicting-mortality
#4
Chen-Jung Wu, Tung-Wei Kao, Yuan-Yuei Chen, Hui-Fang Yang, Wei-Liang Chen
Objective To elucidate whether the PF-combined definition of metabolic syndrome (MetS) would show a better predictive ability for cause-specific mortality than the common MetS. Methods The data were derived from the National Health and Nutrition Examination Survey from 1999 through 2002. We investigated three types of MetS including WCMetS, PFMetS, and PF-combined definition MetS. The main outcome was to evaluate the predictive ability of the newly defined MetS for time to cause-specific mortality. The secondary outcomes were the relationships between the PF percentage and C-reactive protein (CRP) and homeostasis model assessment of insulin resistance (HOMA-IR) to clarify the gender discrepancy...
December 13, 2018: Diabetes/metabolism Research and Reviews
https://www.readbyqxmd.com/read/30548320/editorial-comorbidities-concomitant-medications-and-potential-drug-drug-interactions-with-interferon-free-direct-acting-anti-viral-agents-in-chronic-hepatitis-c-authors-reply
#5
EDITORIAL
Chen-Hua Liu, Jia-Horng Kao
No abstract text is available yet for this article.
January 2019: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/30544928/intracellular-iron-chelation-by-a-novel-compound-c7-reactivates-epstein%C3%A2-barr-virus-ebv-lytic-cycle-via-the-erk-autophagy-axis-in-ebv-positive-epithelial-cancers
#6
Stephanie Pei Tung Yiu, Kwai Fung Hui, Chung King Choi, Richard Yi Tsun Kao, Chi Wang Ma, Dan Yang, Alan Kwok Shing Chiang
Pharmaceutical reactivation of lytic cycle of Epstein⁻Barr virus (EBV) represents a potential therapeutic strategy against EBV-associated epithelial malignancies, e.g., gastric carcinoma (GC) and nasopharyngeal carcinoma (NPC). A novel lytic-inducing compound, C7, which exhibits structural similarity to Di-2-Pyridyl Ketone 4, 4-Dimethyl-3-Thiosemicarbazone (Dp44mT), a known chelator of intracellular iron, is found to reactivate EBV lytic cycle in GC and NPC. This study aims to investigate the role of intracellular iron chelation by C7 and other iron chelators in lytic reactivation of EBV in GC and NPC...
December 11, 2018: Cancers
https://www.readbyqxmd.com/read/30543473/development-of-injury-risk-models-to-guide-ct-evaluation-in-the-emergency-department-after-motor-vehicle-collisions
#7
Thomas Hartka, George Glass, Christopher Kao, Timothy McMurry
OBJECTIVE: The clinical evaluation of motor vehicle collision (MVC) victims is challenging and commonly relies on computed tomography (CT) to detect internal injuries. CT scans are financially expensive and each scan exposes the patient to additional ionizing radiation with an associated, albeit low, risk of cancer. Injury risk prediction based on regression modeling has been to be shown to be successful in estimating Injury Severity Scores (ISSs). The objective of this study was to (1) create risk models for internal injuries of occupants involved in MVCs based on CT body regions (head, neck, chest, abdomen/pelvis, cervical spine, thoracic spine, and lumbar spine) and (2) evaluate the performance of these risk prediction models to predict internal injury...
December 13, 2018: Traffic Injury Prevention
https://www.readbyqxmd.com/read/30541929/a-statistical-procedure-for-genome-wide-detection-of-qtl-hotspots-using-public-databases-with-application-to-rice
#8
Man-Hsia Yang, Dong-Hong Wu, Chen-Hung Kao
Genome-wide detection of quantitative trait loci (QTL) hotspots underlying variation in many molecular and phenotypic traits has been a key step in various biological studies since the QTL hotspots are highly informative and can be linked to the genes for the quantitative traits. Several statistical methods have been proposed to detect QTL hotspots. These hotspot detection methods rely heavily on permutation tests performed on summarized QTL data or individual-level data (with genotypes and phenotypes) from the genetical genomics experiments...
December 12, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/30541863/bluetongue-virus-vp6-and-genomic-rna-interaction-is-essential-for-genome-packaging
#9
Po-Yu Sung, Robert Vaughan, Shah Kamranur Rahman, Guanghui Yi, Adeline Kerviel, C Cheng Kao, Polly Roy
The genomes of the Reoviridae , including the animal pathogen Bluetongue virus (BTV), are multi-segmented double-stranded (ds) RNA. During replication, single-stranded (ss) positive-sense RNA segments are packaged into the assembling virus capsid, triggering genomic dsRNA synthesis. However, exactly how this packaging event occurs is not clear. A minor capsid protein VP6, unique for the orbiviruses, has been proposed to be involved in the RNA packaging process. In this study, we sought to characterize the RNA binding activity of VP6 and its functional relevance...
December 12, 2018: Journal of Virology
https://www.readbyqxmd.com/read/30541586/central-mechanisms-of-real-and-sham-electroacupuncture-in-the-treatment-of-chronic-low-back-pain-study-protocol-for-a-randomized-placebo-controlled-clinical-trial
#10
Jiang-Ti Kong, Brandon MacIsaac, Ruti Cogan, Amanda Ng, Christine Sze Wan Law, Joseph Helms, Rosa Schnyer, Nicholas Vasilis Karayannis, Ming-Chih Kao, Lu Tian, Beth D Darnall, James J Gross, Sean Mackey, Rachel Manber
BACKGROUND: Chronic low back pain (CLBP) is the most common chronic pain condition and is often resistant to conventional treatments. Acupuncture is a popular alternative for treating CLBP but its mechanisms of action remain poorly understood. Evidence suggests that pain regulatory mechanisms (particularly the ascending and secondarily the descending pain modulatory pathways) and psychological mechanisms (e.g., expectations, pain catastrophizing and self-efficacy) may be involved in the pathogenesis of CLBP and its response to treatments...
December 13, 2018: Trials
https://www.readbyqxmd.com/read/30540837/penetration-of-new-antidiabetic-medications-in-east-asian-countries-and-the-united-states-a-cross-national-comparative-study
#11
Kiyoshi Kubota, Yukari Kamijima, Yea-Huei Kao Yang, Shinya Kimura, Edward Chia-Cheng Lai, Kenneth K C Man, Patrick Ryan, Martijn Schuemie, Paul Stang, Chien-Chou Su, Ian C K Wong, Yinghong Zhang, Soko Setoguchi
BACKGROUND: The number of patients with diabetes is increasing particularly in Asia-Pacific region. Many of them are treated with antidiabetics. As the basis of the studies on the benefit and harm of antidiabetic drugs in the region, the information on patterns of market penetration of new classes of antidiabetic medications is important in providing context for subsequent research and analyzing and interpreting results. METHODS: We compared penetration patterns of dipeptidyl peptidase-4 (DPP-4) inhibitors in Taiwan, Hong Kong, Japan, and the United States...
2018: PloS One
https://www.readbyqxmd.com/read/30539517/apasl-clinical-practice-recommendation-how-to-treat-hcv-infected-patients-with-renal-impairment
#12
EDITORIAL
Tatsuo Kanda, George K K Lau, Lai Wei, Mitsuhiko Moriyama, Ming-Lung Yu, Wang-Long Chuang, Alaaeldin Ibrahim, Cosmas Rinaldi Adithya Lesmana, Jose Sollano, Manoj Kumar, Ankur Jindal, Barjesh Chander Sharma, Saeed S Hamid, A Kadir Dokmeci, Mamun-Al-Mahtab, Geofferey W McCaughan, Jafri Wasim, Darrell H G Crawford, Jia-Horng Kao, Osamu Yokosuka, Shiv Kumar Sarin, Masao Omata
Chronic hepatitis C virus (HCV) infection is common among patients with chronic kidney disease (CKD) and those on hemodialysis due to nosocomial infections and past blood transfusions. While a majority of HCV-infected patients with end-stage renal disease are asymptomatic, some may ultimately experience decompensated liver diseases and hepatocellular carcinoma. Administration of a combination of elbasvir/grazoprevir for 12 weeks leads to high sustained virologic response (SVR) rates in patients with HCV genotypes (GTs) 1a, 1b or 4 and stage 4 or 5 CKD...
December 11, 2018: Hepatology International
https://www.readbyqxmd.com/read/30535031/vidian-neurectomy-for-management-of-chronic-cluster-headache
#13
Shao-Cheng Liu, Ming-Chang Kao, Yun-Chen Huang, Wan-Fu Su
BACKGROUND: Management of chronic cluster headache (CCH) remains a challenging endeavor, and the optimal surgical approach for medically refractory CCH remains controversial. OBJECTIVE: To conduct a preliminary evaluation of the efficacy and safety of vidian neurectomy (VN) in patients with medically refractory CCH. METHODS: Between March 2013 and December 2015, 9 CCH patients, all of whom had failed to respond to conservative therapy, underwent VN with a precise nerve cut and maximal preservation of the sphenopalatine ganglion...
December 10, 2018: Neurosurgery
https://www.readbyqxmd.com/read/30533044/exploring-the-link-between-metabolic-syndrome-and-risk-of-dysmobility-syndrome-in-elderly-population
#14
Yuan-Yuei Chen, Tung-Wei Kao, Chung-Ching Wang, Ying-Jen Chen, Chen-Jung Wu, Wei-Liang Chen
Dysmobility syndrome (DMS) was considered as a comprehensive approach to evaluate the condition of musculoskeletal system and adverse health problems in older population. The objective of our study was to examine the association between metabolic syndrome (MetS) and DMS in a U.S. adult population. 1760 eligible participants from the National Health and Nutrition Examination Survey (NHANES) 1999-2002 were enrolled in the study. The criteria of DMS consisted of six domains including increased body fat, declined muscle mass, reduced muscle strength, osteoporosis, slow gait speed, and balance problem...
2018: PloS One
https://www.readbyqxmd.com/read/30531329/accurate-risk-stratification-for-development-of-organ-space-surgical-site-infections-after-emergent-trauma-laparotomy
#15
Shuyan Wei, Charles Green, Lillian S Kao, Brandy B Padilla-Jones, Van T T Truong, Charles E Wade, John A Harvin
BACKGROUND: Organ/space surgical site infection (OS-SSI) develops frequently after trauma laparotomies and is associated with significant morbidity. No valid model exists to accurately risk-stratify the probability of OS-SSI development after emergent laparotomy. Risk stratification for OS-SSI in these patients could guide promising, but unproven, interventions for OS-SSI prevention, such as more frequent dosing of intraoperative antibiotics or direct peritoneal resuscitation.We hypothesize that in trauma patients who undergo emergent laparotomy, probability of OS-SSI can be accurately estimated using patient data available during the index operation...
December 6, 2018: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/30528909/real-time-local-oxygen-measurements-for-high-resolution-cellular-imaging
#16
Liron Boyman, George S B Williams, Andrew P Wescott, Jennie B Leach, Joseph P Y Kao, W Jonathan Lederer
Single-cell metabolic investigations are hampered by the absence of flexible tools to measure local partial pressure of O2 (pO2 ) at high spatial-temporal resolution. To this end, we developed an optical sensor capable of measuring local pericellular pO2 for subcellular resolution measurements with confocal imaging while simultaneously carrying out electrophysiological and/or chemo-mechanical single cell experiments. Here we present the OxySplot optrode, a ratiometric fluorescent O2 -micro-sensor created by adsorbing O2 -sensitive and O2 -insensitive fluorophores onto micro-particles of silica...
December 5, 2018: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/30527566/efficacy-and-safety-of-12-weeks-of-daclatasvir-asunaprevir-plus-ribavirin-for-hcv-genotype-1b-infection-without-ns5a-resistance-associated-substitutions
#17
Ming-Lung Yu, Chao-Hung Hung, Yi-Hsiang Huang, Cheng-Yuan Peng, Chun-Yen Lin, Pin-Nan Cheng, Rong-Nan Chien, Shih-Jer Hsu, Chen-Hua Liu, Chung-Feng Huang, Chien-Wei Su, Jee-Fu Huang, Chun-Jen Liu, Jia-Horng Kao, Wan-Long Chuang, Pei-Jer Chen, Ding-Shinn Chen
BACKGROUND/PURPOSE: Treatment with daclatasvir plus asunaprevir (DCV + ASV) for 24 weeks provided a sustained virologic response (SVR) rate of over 90% in hepatitis C virus genotype 1b (HCV-1b) infected patients without non-structural 5A (NS5A) resistance-associated substitutions (RASs) at the L31 and Y93 sites. In this study, we investigated whether adding ribavirin to the DCV + ASV combination could shorten the original treatment regimen to 12 weeks without compromising the treatment efficacy for HCV-1b patients without NS5A RASs...
December 4, 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/30527501/impact-of-social-media-on-community-consultation-in-exception-from-informed-consent-clinical-trials
#18
John A Harvin, Jeanette M Podbielski, Laura E Vincent, Mike K Liang, Lillian S Kao, Charles E Wade, John B Holcomb
BACKGROUND: Exception from informed consent (EFIC) allows clinician scientists to perform much needed emergency research. Obtaining this exception, however, requires many meetings with community groups for consultation, which can make the process time-consuming and expensive. We aim to determine the impact of using social media in lieu of some community meetings in an effort to obtain an EFIC. MATERIALS AND METHODS: An economic analysis of four randomized clinical trials was performed...
February 2019: Journal of Surgical Research
https://www.readbyqxmd.com/read/30527436/taiwan-consensus-statement-on-the-management-of-chronic-hepatitis-b
#19
Rong-Nan Chien, Jia-Horng Kao, Cheng-Yuan Peng, Chien-Hung Chen, Chun-Jen Liu, Yi-Hsiang Huang, Tsung-Hui Hu, Hwa-I Yang, Sheng-Nan Lu, Yen-Hsuan Ni, Won-Long Chuang, Chuan-Mo Lee, Jaw-Chin Wu, Pei-Jer Chen, Yun-Fan Liaw
The experts of Taiwan Association for the Study of Liver (TASL) have actively participated and led the guidelines on hepatitis B virus (HBV) management by Asian Pacific Association for the Study of Liver (APASL) which is the first international association for the study of liver to publish the statement on HBV management before. However, there are more and more new data on the natural history and treatment of HBV infection in the past decade. These include new application of an old biomarker (quantitative HBsAg), clinical significance of HBV genotype and naturally occurring mutations, the role of non-invasive examination in evaluating severity of hepatic fibrosis, clinical significance of outcome calculators, new drug or new combination strategies towards more effective therapy and organ transplantation including liver and non-liver transplantation...
December 5, 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/30526672/a-novel-spontaneous-hepatocellular-carcinoma-mouse-model-for-studying-t-cell-exhaustion-in-the-tumor-microenvironment
#20
Yu-Tzu Liu, Tai-Chung Tseng, Ruey-Shyang Soong, Chun-Yi Peng, Yu-Hsing Cheng, Shiu-Feng Huang, Tsung-Hsien Chuang, Jia-Horng Kao, Li-Rung Huang
Immunotherapy has ushered in a new era of cancer therapy, and this is also applicable to therapy of hepatocellular carcinoma (HCC). In this context, effective development of therapeutic strategies requires an HCC mouse model with known tumor-associated antigens (TAAs) and an HCC growth reporter. We created such a model using hydrodynamic injection and a transposon system to introduce AKT and NRAS and open reading frames (ORFs) encoding surrogate tumor antigens and luciferase into chromosomes of hepatocytes to induce nodular and diffuse tumors in the liver...
December 7, 2018: Journal for Immunotherapy of Cancer
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