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Xiuyuan Li, Zenglei Zhang, Hua Jiang, Qiang Li, Ruliang Wang, Hongliang Pan, Yingying Niu, Fenghai Liu, Hongmei Gu, Xingjun Fan, Jinxia Gao
BACKGROUND/AIMS: Circular RNAs (circRNAs) are key regulators in the development and progression of human cancers, however its role in non-small cell lung cancer (NSCLC) tumorigenesis is not well understood. The aim of this study is to identify the expression level of circPVT1 in NSCLC and further investigated its functional relevance with NSCLC progression both in vitro and in vivo. METHODS: Quantative real-time PCR was used for the measurement of circPVT1 in NSCLC specimens and cell lines...
2018: Cellular Physiology and Biochemistry
Yong An, Huihua Cai, Yue Zhang, Shengyong Liu, Yunfei Duan, Donglin Sun, Xuemin Chen, Xiaozhou He
BACKGROUND/AIMS: We aimed to study the involvement of circZMYM2 (hsa_circ_0099999) in pancreatic cancer (PC) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circZMYM2 regulating miR-335-5p and JMJD2C. METHODS: CircRNA differential expressions in twenty PC samples and paired normal tissue samples were analyzed using Arraystar Human CircRNA microarray V1. CircZMYM2 expression level was determined via qRT-PCR. The effects of circZMYM2 inhibition and overexpression on cell proliferation, cell apoptosis and cell invasion were investigated by CCK-8 assays, Flow cytometry assays and Transwell assays...
2018: Cellular Physiology and Biochemistry
Isabele Picada Emanuelli, Camila Bortoliero Costa, Luciana Simões Rafagnin Marinho, Marcelo Marcondes Seneda, Flávio Vieira Meirelles
This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage...
November 26, 2018: Theriogenology
Tatjana Ćosić, Martin Raspor, Jelena Savić, Aleksandar Cingel, Dragana Matekalo, Snežana Zdravković-Korać, Slavica Ninković
Kohlrabi (Brassica oleracea var. gongylodes) is an important vegetable crop that is able to undergo shoot regeneration in culture from intact seedlings in a single-step regeneration process, using cytokinin as the only plant growth regulator. In this work, we present the expression profiles of seven organogenesis-related genes over the time course of shoot regeneration from intact seedlings of kohlrabi cv. Vienna Purple on shoot regeneration media containing trans-zeatin, cis-zeatin, benzyl adenine or thidiazuron...
November 8, 2018: Journal of Plant Physiology
Kai Fan, Qunfeng Zhang, Meiya Liu, Lifeng Ma, Yuanzhi Shi, Jianyun Ruan
Tea flowering in late autumn competes for a large amount of nitrogen and carbohydrates, potentially undermines the storage of these resources in vegetative organs, and negatively influences the subsequent spring tea yield and quality. The mechanism underlying the re-allocation N and carbohydrate from source leaf to flower in tea plant has not been clearly understood. In this study, 15 N allocation, changes in metabolomics, and gene expression in flower buds, flowers, and adjacent leaves were characterized. Total N content of the adjacent leaves significantly decreased during flowering while such a decrease could be reversed by flower bud removal...
November 10, 2018: Journal of Plant Physiology
Esther Herrmann, Jan M Engelmann, Michael Tomasello
Humans cultivate their reputations as good cooperators, sometimes even competing with group mates, to appear most cooperative to individuals during the process of selecting partners. To investigate the ontogenetic origins of such "competitive altruism," we presented 5- and 8-year-old children with a dyadic sharing game in which both children simultaneously decided how many rewards to share with each other. The children were either observed by a third-person peer or not. In addition, the children either knew that one of them would be picked for a subsequent collaborative game or had no such knowledge...
December 8, 2018: Journal of Experimental Child Psychology
Jacob A Brockerman, Mark Okon, Stephen G Withers, Lawrence P McIntosh
T4 phage lysozyme (T4L) is an enzyme that cleaves bacterial cell wall peptidoglycan. Remarkably, the single substitution of the active site Thr26 to a His (T26H) converts T4L from an inverting to a retaining glycoside hydrolase with transglycosylase activity. It has been proposed that T26H-T4L follows a double displacement mechanism with His26 serving as a nucleophile to form a covalent glycosyl-enzyme intermediate (Kuroki et al. (1999) PNAS 96: 8949-8954). To gain further insights into this or alternative mechanisms, we used NMR spectroscopy to measure the acid dissociation constants (pKa values) and/or define the ionization states of the Asp, Glu, His, and Arg residues in the T4L mutant...
December 7, 2018: Protein Science: a Publication of the Protein Society
Claudia Escano-Calderón, Neta Rotem, Raviv Harris, David Vela-Corcía, Maggie Levy
Many types of yeasts have been studied in the last few years as potential biocontrol agents against different phytopathogenic fungi. Their ability to control plant diseases is mainly through combined modes of action. Among them, antibiosis, competition for nutrients and niches, induction of systemic resistance in plants and mycoparasitism have been the most studied. In previous work, we have established that the epiphytic yeast Pseudozyma aphidis inhibits Botrytis cinerea through induced resistance and antibiosis...
December 10, 2018: Molecular Plant Pathology
Leah J McGrath, Rohini K Hernandez, Robert Overman, Diane Reams, Alexander Liede, M Alan Brookhart, Elizabeth O'Donnell
BACKGROUND: Prior to 2018, intravenous bisphosphonates (IV BPs) were the only therapies recommended to prevent skeletal-related events for patients diagnosed with multiple myeloma (MM). We examined patterns of IV BP initiation and interruption among patients with newly diagnosed MM (NDMM) in the United States. METHODS: Electronic health records linked to administrative health insurance claims were used to identify adults with NDMM between 1 January 2011 and 30 April 2016...
December 8, 2018: Cancer Medicine
Xiaowei Hu, Yang Li, Dan Kong, Longhu Hu, Donghui Liu, Jin Wu
The long noncoding RNA cancer susceptibility 9 (CASC9) has been reported to be a pivot modulator in growth and metastasis of breast cancer, liver cancer, esophageal squamous cell carcinoma, lung adenocarcinoma, gastric cancer, and nasopharyngeal cancer. However, its potential roles in ovarian cancer remain unclear. In this study, we aimed at its functions and molecular mechanism in ovarian cancer progression. We showed that CASC9 was highly expressed in ovarian cancer tissues and cell lines. An elevated level of CASC9 predicts an unfavorable prognosis in patients with ovarian cancer...
December 7, 2018: Journal of Cellular Physiology
Rasoul Abdollahzadeh, Abdolreza Daraei, Yaser Mansoori, Masoumeh Sepahvand, Mahsa M Amoli, Javad Tavakkoly-Bazzaz
Breast cancer (BC) is the most frequently occurring malignancy in women worldwide. Despite the substantial advancement in understanding the molecular mechanisms and management of BC, it remains the leading cause of cancer death in women. One of the main reasons for this obstacle is that we have not been able to find the Achilles heel for the BC as a highly heterogeneous disease. Accumulating evidence has revealed that noncoding RNAs (ncRNAs), play key roles in the development of BC; however, the involving of complex regulatory interactions between the different varieties of ncRNAs in the development of this cancer has been poorly understood...
December 10, 2018: Journal of Cellular Physiology
Terrence E Murphy, Thomas M Gill, Linda S Leo-Summers, Evelyne A Gahbauer, Margaret A Pisani, Lauren E Ferrante
OBJECTIVES: To address the competing risk of death in longitudinal studies of older persons, we demonstrate sensitivity analyses that evaluate robustness of associations between exposures and three outcome types: dichotomous, count, and time to event. DESIGN: A secondary analysis of data from a prospective cohort study. SETTING: Community-based data from the Precipitating Events Project in New Haven, CT. PARTICIPANTS: Persons 70 years and older who were initially community dwelling and without disability in the four basic activities of daily living (N = 754)...
December 8, 2018: Journal of the American Geriatrics Society
Changhui Gao, Wuhao Lu, Weihua Lou, Liang Wang, Qiang Xu
Long noncoding RNAs have been reported to be important regulators in numerous cancers. In this study, we found that HOXC13 antisense RNA (HOXC13-AS) was highly expressed in head and neck squamous carcinoma (HNSC) tissues in The Cancer Genome Atlas database. Nasopharyngeal carcinoma (NPC) belongs to HNSC. Therefore, we further investigated the potential role of HOXC13-AS in NPC. Quantitative reverse transcription polymerase chain reaction examination revealed that HOXC13-AS was markedly upregulated in NPC tissues and cell lines...
December 7, 2018: Journal of Cellular Physiology
P A Sander van de Hoef, M S Michel Brink, B M A Bionka Huisstede, M Maarten van Smeden, N Niels de Vries, E A Edwin Goedhart, V Vincent Gouttebarge, F J G Frank Backx
BACKGROUND: Although the Nordic Hamstring Exercise (NHE) prevents hamstring injury in soccer players effectively, the annual incidence of these injuries still increases. This may be because of poor long-term compliance with the program. Furthermore, the timing and amplitude of gluteal and core muscle activation seem to play an important role in hamstring injury prevention, the NHE program was not designed to improve activation of these muscles. Therefore, we propose plyometric training as an alternative to reduce hamstring injuries soccer players...
December 9, 2018: Scandinavian Journal of Medicine & Science in Sports
Magali Krzemien, Christelle Maillart, Christophe Parisse, Sandrine Leroy
BACKGROUND: Analogical mapping is a domain-general cognitive process used notably in language development, and particularly in the abstraction of construction schemas. Children with developmental language disorders (DLD) display an impairment in linguistic productivity and creativity, which can be linked to a lack of generalization of construction schemas. AIMS: To investigate analogical mapping in children with DLD, and especially the influence of processing load, as it could explain the lack of creativity observed in children with DLD...
December 10, 2018: International Journal of Language & Communication Disorders
Ray Dybzinski, Angelo Kelvakis, John McCabe, Samantha Panock, Kanyarak Anuchitlertchon, Leah Vasarhelyi, M Luke McCormack, Gordon G McNickle, Hendrik Poorter, Clare Trinder, Caroline E Farrior
Understanding the effects of global change in terrestrial communities requires an understanding of how limiting resources interact with plant traits to affect productivity. Here, we focus on nitrogen and ask whether plant community nitrogen uptake rate is determined (i) by nitrogen availability alone or (ii) by the product of nitrogen availability and fine-root mass. Surprisingly, this is not empirically resolved. We performed controlled microcosm experiments and reanalyzed published pot experiments and field data to determine the relationship between community-level nitrogen uptake rate, nitrogen availability, and fine-root mass for 46 unique combinations of species, nitrogen levels, and growing conditions...
December 7, 2018: Global Change Biology
Regina Berjano, Nancy L Rodríguez-Castañeda, Pedro L Ortiz, María A Ortiz, Montserrat Arista
PREMISE OF THE STUDY: Although an evolutionary link between breeding system and dispersibility has been proposed, to date empirical data and theoretical models of plants show contrasting trends. METHODS: We tested two competing hypotheses for the association between breeding systems and dispersibility in the heterocarpic Hypochaeris salzmanniana (Asteraceae) by using both an experimental approach and surveys over 2 years of five natural populations along an environmental cline with a gradient of pollinator availability...
December 11, 2018: American Journal of Botany
Jing Wang, Ming Zhang, Weifeng Lu
The long noncoding RNA (lncRNA) gastric cancer associated transcript 3 (GACAT3) has been reported to play important roles in human tumorigenesis. However, its expression pattern, functions, and underlying mechanism in glioma remain unclear. In the present study, we showed that GACAT3 is upregulated in glioma tissues and cell lines. Through online databases, luciferase reporter assays and RNA immunoprecipitation (RIP) assays, we determined that GACAT3 acts as a competing endogenous RNA (ceRNA) for microRNA (miR)-135a, which was downregulated and performed as a tumor inhibitor in glioma...
December 10, 2018: Journal of Cellular Physiology
T Engelhardt, D Ayansina, G T Bell, V Oshan, J S Rutherford, N S Morton
The anaesthesia practice in children observational trial of 31,127 patients in 261 European hospitals revealed a high (5.2%) incidence of severe critical events in the peri-operative period and wide variability in practice. A sub-analysis of the UK data was undertaken to investigate differences compared with the non-UK cohort in the incidence and nature of peri-operative severe critical events and to attempt to identify areas for quality improvement. In the UK cohort of 7040 paediatric patients from 43 hospitals, the overall incidence of peri-operative severe critical events was lower than in the non-UK cohort (3...
December 10, 2018: Anaesthesia
Alexander Strauss, Jan-Thorsten Gräsner
Maternal emergencies during pregnancy are characterized by a concomitant threat to 2 patients. These emergencies can occur during or even before a hospital stay. Due to the rarity of the latter cases, sustainable methods of knowledge transfer should be implemented so emergency teams have sufficient obstetrical information to provide emergency care during pregnancy. In a series of articles, launched here, individual competencies for prehospital emergency management of pregnant women are described. Part one addresses prehospital emergency care of pregnant women, their emergency transport, handover management on hospital admission, and a team approach to perinatal emergency care...
December 2018: Zeitschrift Für Geburtshilfe und Neonatologie
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