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rheumatoid arthritis synovial fibroblast

Hu-Nan Sun, Ying-Hua Luo, Ling-Qi Meng, Xian-Ji Piao, Yue Wang, Jia-Ru Wang, Hao Wang, Yi Zhang, Jin-Qian Li, Wan-Ting Xu, Yang Liu, Yu Zhang, Tong Zhang, Ying-Hao Han, Mei-Hua Jin, Gui-Nan Shen, Yan-Qing Zang, Long-Kui Cao, Dong-Jie Zhang, Cheng-Hao Jin
The present study investigated the mechanisms of apoptosis induced by cryptotanshinone (CT) in human rheumatoid arthritis fibroblast‑like synoviocytes (RA‑FLSs). Cell Counting kit‑8 assay was performed to determine the cytotoxic effects of CT in human RA‑FLSs, including primary RA‑FLS, HFLS‑RA and MH7A cells, and in HFLS cells derived from normal synovial tissue. Annexin V‑FITC/PI staining was used to detect the apoptotic effects of CT in HFLS‑RA and MH7A cells. Flow cytometry was performed to detect the apoptotic and reactive oxygen species (ROS) levels induced by CT in HFLS‑RA cells...
December 3, 2018: International Journal of Molecular Medicine
Heidi L Reesink, Alan J Nixon, Jin Su, Sherry Liu, Ryan M Sutton, Sabine Mann, Ashlee E Watts, Ryan P Peterson
Galectins are potent regulators of cell adhesion, growth and apoptosis in diverse cell types, including chondrocytes and synovial fibroblasts. Elevations in synovial fluid galectin-3 have been observed in rheumatoid arthritis, juvenile idiopathic arthritis and experimental inflammatory arthritis in animal models, whereas galectin-1 is thought to be protective. Less is known about galectins-1 and-3 in osteoarthritis (OA). Therefore, the purpose of this study was: (1) to determine whether galectin-1 and-3 synovial fluid concentrations and synovial membrane and cartilage histochemical staining were altered following osteochondral injury in an experimental equine osteoarthritis (OA) model and (2) to measure galectin-1 and-3 mRNA expression and synovial fluid concentrations in naturally occurring equine carpal OA...
2018: Frontiers in Veterinary Science
Ryoji Fujii, Rie Komatsu, Tomoo Sato, Iwao Seki, Koji Konomi, Hiroyuki Aono, Hisateru Niki, Kazuo Yudoh, Kusuki Nishioka, Toshihiro Nakajima
This study was performed to elucidate the molecular function of the synoviocyte proliferation-associated in collagen-induced arthritis (CIA) 1/serum amyloid A-like 1 (SPACIA1/SAAL1) in mice CIA, an animal model of rheumatoid arthritis (RA), and human RA-synovial fibroblasts (RASFs). SPACIA1/SAAL1 -deficient mice were generated and used to create mouse models of CIA in mild or severe disease conditions. Cell cycle-related genes, whose expression levels were affected by SPACIA1/SAAL1 small interfering RNA (siRNA), were screened...
November 30, 2018: International Journal of Molecular Sciences
Kosuke Yamaguchi, Haruka Sudo, Kazushi Imai
An increase in the vasculature is one of most representative changes in the synovial tissue of joints in rheumatoid arthritis (RA) and is closely associated with disease progression. Although the vasculatures are believed to be a result of VE-cadherin-dependent angiogenesis and a possible therapeutic target of the disease, synovial fibroblastic cells express VE-cadherin and form tube-like structures, suggesting that vasculatures in RA synovium may not simply result from angiogenesis. This paper analyzes a mechanism of VE-cadherin expression by rheumatoid arthritic synovial fibroblast-like cells (RSFLs) and their involvement in the tube-like formation...
November 28, 2018: Biochemical and Biophysical Research Communications
Xiaofeng Guo, Dongmei Zhang, Xing Zhang, Jiawei Jiang, Pengfei Xue, Chunshuai Wu, Jinlong Zhang, Guohua Jin, Zhen Huang, Jian Yang, Xinhui Zhu, Wei Liu, Guanhua Xu, Zhiming Cui, Guofeng Bao
Fibroblast-like synoviocytes (FLSs) play an essential role in rheumatoid arthritis (RA) by promoting synovitis, pannus growth and cartilage/bone destruction. Increased proliferation, migration and invasion of FLSs greatly contribute to RA initiation and progression. Dual-specificity tyrosine-regulated kinase 1A (Dyrk1A), a serine/threonine kinase, regulates MAPK pathway activation, and governs the proliferation and differentiation of neuronal progenitor cells and cancer cells. Till now, the expression and possible function of Dyrk1A in RA FLSs have not been explored...
December 2018: Tissue & Cell
Yujie Dai, Jingjing Ding, Wen Yin, Yuzhu He, Fei Yu, Cong Ye, Shaoxian Hu, Yikai Yu
Tumor Necrosis Factor-alpha (TNF- α ) was reported to increase autophagy in rheumatoid arthritis human fibroblast-like synovial cell (RA-HFLS). We investigated different levels of TNF- α exposed to RA-HFLS by focusing on the relationship of autophagy and apoptosis. RA-HFLS and normal human fibroblast-like synovial cell (HFLS) were stimulated by TNF- α in the presence or the absence of 3-methyladenine (3-MA) or chloroquine (CQ). Cell apoptosis was detected by flow cytometry. Autophagy was determined through the expression levels of LC3, Beclin1, and P62 measured by Western Blot analysis as well as Confocal Laser Scanning Microscopy...
2018: BioMed Research International
Felix I L Clanchy, Richard O Williams
OBJECTIVE: Ibudilast is a well-tolerated, orally available type 4 phosphodiesterase (PDE4) inhibitor used to treat asthma and stroke. As PDE4 inhibition suppresses inflammatory mediator production and cell proliferation in leukocytes, ibudilast may be a valuable therapy for the treatment of inflammatory auto-immune diseases such as rheumatoid arthritis (RA). We assessed the therapeutic potential of ibudilast by measuring its capacity to modulate inflammation in human leukocytes, RA synovial fibroblasts (RASF) and in experimental arthritis...
November 26, 2018: Arthritis & Rheumatology
Charles J Malemud
Rheumatoid arthritis (RA) is a chronic, progressive, systemic autoimmune disease that mostly affects small and large synovial joints. At the molecular level, RA is characterized by a profoundly defective innate and adaptive immune response that results in a chronic state of inflammation. Two of the most significant alterations in T-lymphocyte (T-cell) dysfunction in RA is the perpetual activation of T-cells that result in an abnormal proliferation state which also stimulate the proliferation of fibroblasts within the joint synovial tissue...
November 22, 2018: Cells
Junxian Ma, Xinbo Wang, Tingting Lv, Jie Liu, Ying Ren, Jinshan Zhang, Yan Zhang
Ghrelin is a circulating peptide hormone, which involved in promoting feeding and regulating energy metabolism in human and rodents. Abnormal synovial hyperplasia is the most important pathologic hallmark of rheumatoid arthritis (RA), which is characterised by tumor-like expansion. Existing studies indicated that there may exist some relation between the decreased ghrelin and the abnormally proliferating synovial cells in RA. Therefore, the aim of this study is to explore the apoptotic effects of ghrelin on MH7A synovial cells in vitro...
November 15, 2018: Biological & Pharmaceutical Bulletin
Vikram Haridas, Praveenkumar Shetty, E Sarathkumar, Anil Bargale, Jamboor K Vishwanatha, Vidya Patil, U S Dinesh
Annexin A2 has been implicated in several immune modulated diseases including Rheumatoid arthritis (RA) pannus formation. The most relied treatment option for RA pathogenesis is glucocorticoids. Glucocorticoids regulate the synthesis, phosphorylation and cellular deposition of Annexin A1. This annexin mediates the anti-inflammatory actions of glucocorticoids. These two first characterized members of annexin superfamily proteins acts reciprocally, one as an anti-inflammatory and the other proinflammatory in nature...
November 13, 2018: Molecular Biology Reports
Xiaodong Wen, Xun Chen, Xiaojun Liang, Hongmou Zhao, Yi Li, Xiangxiang Sun, Jun Lu
The activation of TNF-α/NF-кB signaling is involved in the regulation of a wide range of biological processes, such as cell proliferation, differentiation and apoptosis, eventually causing a number of diseases, such as cancer and inflammation. Here, we found that TNF-α/NF-кB signaling was activated in a large number of blood samples taken from foot and ankle rheumatoid arthritis (RA) patients. By applying a microarray assay to the human synovial sarcoma cell line SW982 and the human fibroblast-like synoviocyte cell line HFLS-RA, as well as in their corresponding p65 knockdown and -overexpressing cells, we identified and verified the activation of many p65 targets, including cytokines (e...
2018: International Journal of Biological Sciences
Di Fu, Chuyin Xiao, Yingying Xie, Jianquan Gao, Shanhui Ye
Rheumatoid arthritis synovial fibroblasts (RASFs) play a key role in the pathogenesis of rheumatoid arthritis (RA). This study was aimed to investigate the effects of miR-3926 on the biological activities of RASFs. The results showed that miR-3926 was significantly down-regulated in RASFs and RA synovial tissue. Overexpression of miR-3926 significantly inhibited RASFs proliferation and decreased the secretion of inflammatory cytokines including TNF-α, IL-1β and IL-6 in RASFs. TLR5 was identified to be a direct target of miR-3926...
November 6, 2018: Gene
Asmaa A Alsousi, Orisa J Igwe
Reactive oxygen species (ROS) are implicated to play a role in initiating rheumatoid arthritis (RA) pathogenesis. We have investigated the mechanism(s) by which essential redox-active trace metals (RATM) may induce cell proliferation and cell death in rabbit synovial fibroblasts. These fibroblast-like synovial (FLS) cells, which express Toll-like receptor 4 (TLR4), were used as a model system that plays a role in potentially initiating RA through oxidative stress. Potassium peroxychromate (PPC, [Cr5+ ]), ferrous chloride (FeCl2 , [Fe2+ ]), and cuprous chloride (CuCl, [Cu+ ]) in the indicated valency states were used as exogenous pro-oxidants that can induce oxidative stress through TLR4 coupled activation that also causes HMGB1 release...
November 6, 2018: Experimental Cell Research
G Zhang, H-B Liu, L Zhou, X-Q Cui, X-H Fan
OBJECTIVE: To investigate whether CC chemokine 3 (CCL3) could exert a certain effect on rheumatoid arthritis (RA) by regulating inflammatory responses and provide a new direction for the treatment of RA. PATIENTS AND METHODS: Totally 47 RA patients (10 males and 37 females) with complete clinical data were included. Meanwhile, 27 healthy volunteers with same age and gender were recruited as healthy controls. The mRNA and protein level of CCL3 in the peripheral blood mononuclear cells (PBMCs) of RA patients and normal controls were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blot, respectively...
October 2018: European Review for Medical and Pharmacological Sciences
Amit Kumar Rana, Yang Li, Qiujie Dang, Fan Yang
Rheumatoid arthritis (RA) is a chronic systemic, autoimmune and inflammatory disease represented as synovitis, pannus formation, adjacent bone erosions, and joint destruction. The major cells involved in the perpetuation of RA pathogenesis are CD4+ T-cells (mainly Th1 cells and Th17 cells), fibroblasts like synoviocytes (FLS), macrophages and B cells. Other autoimmune cells such as dendritic cells, neutrophils, mast cells, and monocytes also contribute to RA pathogenesis. Monocytes are mainly bone marrow (BM) derived cells in the circulation...
December 2018: International Immunopharmacology
Hidetoshi Fujita, Satoko Aratani, Naoko Yagishita, Kusuki Nishioka, Toshihiro Nakajima
Synoviolin (Syvn1), an E3 ubiquitin ligase in endoplasmic reticulum‑associated protein degradation, is involved in rheumatoid arthritis, fibrosis, liver cirrhosis and obesity. We previously demonstrated that Syvn1 negatively regulates the function of peroxisome proliferator‑activated receptor gamma coactivator‑1β (PGC‑1β). In addition, treatment with a Syvn1 inhibitor suppressed weight gain in a mouse model of obesity by activating PGC‑1β via Syvn1 inhibition. It has been suggested that the Syvn1 inhibitors may have therapeutic benefits in obese patients...
December 2018: Molecular Medicine Reports
María Ángeles Rosillo, Catalina Alarcón-de-la-Lastra, María Luisa Castejón, Tatiana Montoya, Marta Cejudo-Guillén, Marina Sánchez-Hidalgo
The polyphenolic extract (PE) from extra virgin olive oil (EVOO) has been shown to possess important anti-inflammatory and joint protective properties in murine models of rheumatoid arthritis (RA). This study was designed to evaluate the effects of PE on IL-1β-activated human synovial fibroblasts SW982 cell line. PE from EVOO treatment inhibited IL-1β-induced matrix metalloproteases (P<0·001), TNF-α and IL-6 production (P<0·001). Similarly, IL-1β-induced cyclo-oxygenase-2 and microsomal PGE synthase-1 up-regulations were down-regulated by PE (P<0·001)...
October 26, 2018: British Journal of Nutrition
Jie Wu, Wenqiang Fan, Ling Ma, Xiuqin Geng
Aim: MicroRNAs (miRNA) are a class of small, highly conserved noncoding RNA molecules, which contain 18-28 nucleotides and are involved in the regulation of gene expression. It has been proved that microRNAs play a very important role in several key cellular processes, such as cell differentiation, cell cycle progression, and apoptosis, as well as in autoimmune disease. One recently identified miRNA, miR-708-5p, has been demonstrated to have profound roles in suppressing oncogenesis in different types of tumors...
2018: Drug Design, Development and Therapy
Chenggui Miao, Jun Chang, Jinfeng Dou, Youyi Xiong, Guoliang Zhou
In this work, the expression of secreted frizzled related protein 2 (SFRP2) in rheumatoid arthritis (RA) model rats and the mechanisms of SFRP2 on the RA pathogenesis were investigated. Data suggested that SFRP2 was significantly down-regulated in RA model rats compared with normal control, and overexpression of SFRP2 suppressed the RA pathogenesis and the canonical Wnt signaling in fibroblast-like synovial cells (FLS) from RA model rats, whereas knockdown of SFRP2 got an opposite observation. Interestingly, 5-azadC treatment up-regulated the SFRP2 expression, inhibited the FLS proliferation, suppressed the expression of IL-6 and IL-8 and the fibronectin production, suggesting that the decreased SFRP2 in RA model rats was due to the DNA methylation...
October 20, 2018: Autoimmunity
Yan Wang, Li Dai, Hong Wu, Zheng-Rong Zhang, Wen-Yu Wang, Jun Fu, Ran Deng, Feng Li, Xue-Jing Dai, Xiang Zhan
Geniposide (GE) is an active component isolated from the fruit of Gardenia jasminoides Ellis that has anti-inflammatory and other pharmacological effects; however, the underlying mechanism of GE action has not been elucidated in rheumatoid arthritis (RA). Previous studies have shown that GE plays a therapeutic role in RA via regulation of the integrin beta 1 (Itgβ1)-mediated Ras-Erk1/2 signalling pathway. However, the specific mechanism of GE action on Itgβ1 has not been clarified. Recent evidence indicates that microRNAs (miRNAs) are involved in the development of RA...
December 2018: International Immunopharmacology
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