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Christopher Howell, James R Smith, Janis K Shute
BACKGROUND: Viral infection of the bronchial epithelium disrupts the barrier properties of the epithelium in healthy individuals and those with lung disease. Repair of the bronchial epithelium is dependent of the formation of a provisional fibrin matrix and migration of epithelial cells to cover denuded areas, followed by proliferation and differentiation. OBJECTIVE: The objective was to test the hypothesis that poly I:C, a model of viral infection, limits epithelial repair through the stimulated release of matrix metalloproteinase-13 (MMP-13)...
June 20, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Rui Ji, Qian Ren, Suyang Bai, Yuping Wang, Yongning Zhou
BACKGROUND: The high pretreatment plasma fibrinogen has been widely reported to be a possible biomarker for predicting prognosis in hepatocellular carcinoma (HCC) and pancreatic carcinoma (PC), but persuasive conclusion has not been made yet. Thus, we herein conducted a meta-analysis to comprehensively assess the prognostic value of high pretreatment plasma fibrinogen in patients with HCC and PC. METHOD: We systematically searched PubMed, EMBASE, and Web of Science to identify eligible studies from inception to November 10, 2017...
June 2018: Medicine (Baltimore)
Kim Maree O'Sullivan, Sharon Lee Ford, Anthony Longano, A Richard Kitching, Stephen R Holdsworth
In anti-neutrophil cytoplasmic antibody associated vasculitis (AAV), toll like receptors (TLRs) may be engaged by infection associated patterns and by endogenous danger signals, linking infection and innate inflammation with this autoimmune disease. This study examined intrarenal TLR2, TLR4 and TLR9 expression and renal injury in AAV, testing the hypothesis that increased TLR expression correlates with renal injury. Patients with AAV exhibited both glomerular and tubulointerstitial expression of TLR2, TLR4 and TLR9, with TLR4 being the most prominent in both compartments...
June 20, 2018: American Journal of Physiology. Renal Physiology
Mirja E Graf, Disorn Sookthai, Theron Johnson, Ruth Schübel, Sandra González Maldonado, Laura Pletsch-Borba, Verena Katzke, Peter Bugert, Michael Hoffmeister, Rudolf Kaaks, Tilman Kühn
While enhanced platelet activation may drive cancer progression and metastases, less is known about its role in early cancer development. Thus, we evaluated whether pre-diagnostic biomarkers of platelet activation and coagulation are related to the risks of common cancers in the prospective EPIC-Heidelberg Study using a case-cohort design. Levels of fibrinogen, soluble glycoprotein (sGP) IIb/IIIa, soluble P-selectin (sP-selectin), soluble thrombomodulin (sTM), and thrombopoietin (TPO) were measured in baseline plasma samples of a random subcohort (n=2,480) and incident cases of breast (n=605), prostate (n=543), and colorectal cancer (n=249)...
June 19, 2018: International Journal of Cancer. Journal International du Cancer
Michael Goldfarb, Lawrence S Czer, Lee D Lam, Jaime Moriguchi, Francisco A Arabia, Oksana Volod
Acquired von Willebrand syndrome (VWS) due to loss of high-molecular-weight multimers (HMWMs) has been reported with longer term mechanical devices and is associated with mucosal bleeding, a primary hemostasis type of bleeding. However, little is known whether a similar defect occurs in patients with short-term mechanical circulatory support (STMCS) devices. We reviewed von Willebrand factor (VWF) profiles in patients with STMCS devices who underwent VWS workup from December 2015 to March 2017 at an academic quaternary care hospital...
June 2018: Journal of Extra-corporeal Technology
Kazuhiro Shirozu, Naoyuki Fujimura, Yuji Karashima, Mizuko Ikeda, Hidehisa Kitada, Yasuhiro Okabe, Kei Kurihara, Tomoko Henzan, Sumio Hoka
BACKGROUND: ABO-incompatible living-donor kidney transplantation (LDKT) requires immunotherapy and plasma exchange therapy (PEX). PEX with albumin replacement fluid reportedly decreases fibrinogen levels. However, no reports have described the effects of PEX with albumin replacement fluid on blood coagulation parameters and blood loss during the perioperative period. Therefore, we investigated the effects of preoperative PEX on blood coagulation parameters and blood loss during the perioperative period in patients undergoing ABO-incompatible LDKT as measured by rotational thromboelastometry (ROTEM®)...
June 19, 2018: BMC Anesthesiology
Samiullah Khan, Aqsa Gul, Rabia Noreen, Muhammad Ashraf, Sohail Ahmad, Sattar Bakhsh Awan
BACKGROUND: Thrombus is composed of two main substances i.e. red blood cells and aggregated platelets which make a web of inter-connected fibrin proteins. During injury it prevents bleeding, so it is very useful but it can be very dangerous if it is produced in healthy blood vessels and block the blood flow through it. Mural thrombi attaches with the blood vessels but in most cases do not block it completely. Venoms are an incredible source of peptides having amazing bioactivities with varying number of amino acid residues...
June 13, 2018: Protein and Peptide Letters
Sarah B Shropshire, Christine S Olver, Michael R Lappin
OBJECTIVE: To assess interindividual (CVG ) and intraindividual (CVI ) variability over time for results of thromboelastography (TEG) and whole-blood impedance platelet aggregometry in healthy dogs. ANIMALS: Six healthy Beagle dogs. MEASUREMENTS AND MAIN RESULTS: Tissue factor (TF)-activated TEG and adenosine diphosphate (ADP) and arachidonic acid (AA)-induced whole blood impedance platelet aggregometry were performed at 3 different time points (days 1, 4, and 6)...
June 19, 2018: Journal of Veterinary Emergency and Critical Care
Zahava Vadasz, Elias Toubi
The involvement of the hemostatic system in immune-mediated inflammation is widely reported. Many coagulation factors play a role in the pathogenesis of autoimmune diseases, such as systemic vasculitis and systemic lupus erythematosus. Hemostatic disorders are also involved in asthma and chronic spontaneous urticaria (CSU). Factor XIIa (FXIIa) was one of the first coagulation factors implicated in inducing both humoral and cellular responses and is therefore considered a prime new therapeutic target in immune-mediated inflammation...
June 19, 2018: Seminars in Thrombosis and Hemostasis
Jonathan H Schultz, Lijing Bu, Coen M Adema
The freshwater snail Physella acuta was selected to expand the perspective of comparative snail immunology. Analysis of Physella acuta, belonging to the Physidae, taxonomic sister family to Planorbidae, affords family-level comparison of immune features characterized from Biomphalaria glabrata, the model snail often used to interpret general gastropod immunity. To capture constitutive and induced immune sequences, transcriptomes of an individual Physella acuta snail, 12 h post injection with bacteria (Gram -/+) and one sham-exposed snail were recorded with 454 pyrosequencing...
June 16, 2018: Molecular Immunology
Seong Eun Jin, Chang-Seob Seo, Mee-Young Lee, Hyeun-Kyoo Shin, Mi-Jin Yang, Hyekyung Ha
ETHNOPHARMACOLOGICAL RELEVANCE: Traditional herbal formula Gyejibokryeong-hwan (GJBRH; Guizhifuling-wan, Keishibukuryo-gan) consisting five medicinal herbs has been used to treat uterine disorders, gynecological diseases and blood stasis syndrome in Asia. AIM OF THE STUDY: We evaluated the safety of GJBRH in Crl:CD Sprague-Dawley (SD) rats over a period of 13 weeks. MATERIALS AND METHODS: To confirm the stability of the components of GJBRH, we analyzed the component contents in GJBRH at different storage periods, using high-performance liquid chromatography...
June 16, 2018: Journal of Ethnopharmacology
Andrea L Grant, Hayley L Letson, Jodie L Morris, Peter McEwen, Kaushik Hazratwala, Matthew Wilkinson, Geoffrey P Dobson
BACKGROUND: Tranexamic acid (TXA) is commonly used in orthopedic surgery to reduce excessive bleeding and transfusion requirements. Our aim was to examine if TXA was required in all osteoarthritis patients undergoing TKA surgery, and its possible effects on systemic inflammation and coagulation properties. METHODS: Twenty-three patients (Oxford Score 22-29) were recruited consecutively; 12 patients received TXA before (IV, 1.2 g/90 kg) and immediately after surgery (intra-articular, 1...
June 18, 2018: Journal of Orthopaedic Surgery and Research
Nicola Curry, Claire Foley, Henna Wong, Ana Mora, Elinor Curnow, Agne Zarankaite, Renate Hodge, Valerie Hopkins, Alison Deary, James Ray, Phil Moss, Matthew J Reed, Suzanne Kellett, Ross Davenport, Simon Stanworth
BACKGROUND: There is increasing interest in the timely administration of concentrated sources of fibrinogen to patients with major traumatic bleeding. Following evaluation of early cryoprecipitate in the CRYOSTAT 1 trial, we explored the use of fibrinogen concentrate, which may have advantages of more rapid administration in acute haemorrhage. The aims of this pragmatic study were to assess the feasibility of fibrinogen concentrate administration within 45 minutes of hospital admission and to quantify efficacy in maintaining fibrinogen levels ≥ 2 g/L during active haemorrhage...
June 18, 2018: Critical Care: the Official Journal of the Critical Care Forum
Brendan J Kelly, Ebbing Lautenbach, Irving Nachamkin, Susan E Coffin, Jeffrey S Gerber, Barry D Fuchs, Charles Garrigan, Xiaoyan Han, Warren B Bilker, Jacqueleen Wise, Pam Tolomeo, Jennifer H Han
OBJECTIVES: Sepsis is associated with high early and total in-hospital mortality. Despite recent revisions in the diagnostic criteria for sepsis that sought to improve predictive validity for mortality, it remains difficult to identify patients at greatest risk of death. We compared the utility of nine biomarkers to predict mortality in subjects with clinically suspected bacterial sepsis. DESIGN: Cohort study. SETTING: The medical and surgical ICUs at an academic medical center...
July 2018: Critical Care Medicine
A Straumfors, W Eduard, K K Heldal, M Skogstad, L Barregård, D G Ellingsen
PURPOSE: To investigate if blood biomarkers could indicate early signs of lung damage or cardiovascular risk due to exposure to grain dust. MATERIALS AND METHODS: Pneumoproteins and markers of inflammation and platelet activation were analyzed in blood samples of 102 grain elevator and compound feed mill workers. Differences between exposed (n = 67) and controls (n = 35), and associations with exposure measurements and respiratory health were investigated by multiple linear regression analyses...
June 18, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Jorien Claes, Bartosz Ditkowski, Laurens Liesenborghs, Tiago Rafael Veloso, Jose M Entenza, Philippe Moreillon, Thomas Vanassche, Peter Verhamme, Marc F Hoylaerts, Ruth Heying
Adhesion of Staphylococcus aureus to endothelial cells (ECs) is paramount in infective endocarditis. Bacterial proteins such as clumping factor A (ClfA) and fibronectin binding protein A (FnbpA) mediate adhesion to EC surface molecules and (sub)endothelial matrix proteins including fibrinogen (Fg), fibrin, fibronectin (Fn) and von Willebrand factor (vWF). We studied the influence of shear flow and plasma on the binding of ClfA and FnbpA (including its sub-domains A, A16+ , ABC, CD) to coverslip-coated vWF, Fg/fibrin, Fn or confluent ECs, making use of Lactococcus lactis , expressing these adhesins heterologously...
June 17, 2018: Thrombosis and Haemostasis
Ying Zhang, Qian Xin, Zhen Wu, Chaochao Wang, Yongbin Wang, Qian Wu, Rui Niu
BACKGROUND The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF). MATERIAL AND METHODS Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers. RESULTS A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments - CRP, fibrinogen-α chain, haptoglobin, and kininogen-1 - were successfully verified using ELISA...
June 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
B Sobolewska, J Golenko, S Poeschel, C Grimmel, A Gatsiou, K Sopova, T Biedermann, K Schenke-Layland, K Stellos, F Ziemssen
Aflibercept appears to accumulate in systemic circulation following intravitreal injections in therapy of neovascular age-related macular degeneration. This gives raise to the question of whether aflibercept affects platelets and their function such as activation and aggregation, which are substantial in the pathogenesis of an arterial thromboembolic event (ATE). In order to determine the effect of aflibercept in platelet activation, platelets from healthy volunteers were treated with aflibercept and its solvents at equal concentrations (0...
June 14, 2018: Experimental Eye Research
Takumi Sano, Yoshito Terai, Atsushi Daimon, Misa Nunode, Yoko Nagayasu, Atsuko Okamoto, Daisuke Fujita, Masami Hayashi, Masahide Ohmichi
INTRODUCTION: Placental insufficiency is one of the major risk factors for growth restriction and preeclampsia. The aim of this study is to investigate whether recombinant human Thrombomodulin(r-TM) improves fetal conditions and physiological outcomes. METHODS: We used CBA/J × BALB/C mice as a control and CBA/J × DBA/2 mice - a well-studied model of recurrent spontaneous miscarriage. Pregnant mice received daily subcutaneous injections of r-TM or saline from day 0-15...
May 2018: Placenta
Mine Altunbek, Seda Keleştemur, Gülin Baran, Mustafa Çulha
Zinc oxide nanoparticles (ZnO) are presented as potential cancer therapeutic agent based on their surface properties. In this study, the most abundant blood proteins, albumin, fibrinogen and apo-transferrin, were covalently bound (c-ZnO NPs) and nonspecifically adsorbed (n-ZnO NPs) onto ZnO NPs to evaluate the role of modification route on protein structure and their effects on glioblastoma cells. The success of modification and structures of proteins on ZnO NPs were characterized with FT-IR. It was found that non-covalent interaction significantly damaged the secondary structure of proteins compared to those covalently attached to the ZnO nanoparticle...
June 13, 2018: International Journal of Biological Macromolecules
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