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JOURNAL ARTICLE
Yiming Sun, Ailan Huang, Zhi Zhao, Chen Song, Guihua Lai
OBJECTIVE: To investigate immunogenic and toxic effects of graphene oxide (GO) nanoparticles in mouse skeletal muscles and in human blood in vitro . METHODS: GO nanoparticles prepared using a probe sonicator were supended in deionized H2 O or PBS, and particle size and surface charge of the nanoparticles were measured with dynamic light scattering (DLS). Different concentrations (0.5, 1.0 and 2.0 mg/mL) of GO suspension or PBS were injected at multiple sites in the gastrocnemius muscle (GN) of C57BL/6 mice, and inflammatory response and immune cell infiltrations were detected with HE and immunofluorescence staining...
April 20, 2024: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
#2
JOURNAL ARTICLE
Huiyue Lin, Juyong Wang, Qing Shi, Minmin Wu
BACKGROUND: This study was performed to determine the biological processes in which NKX2-1 is involved and thus its role in the development of lung squamous cell carcinoma (LUSC) toward improving the prognosis and treatment of LUSC. METHODS: Raw RNA sequencing (RNA-seq) data of LUSC from The Cancer Genome Atlas (TCGA) were used in bioinformatics analysis to characterize NKX2-1 expression levels in tumor and normal tissues. Survival analysis of Kaplan-Meier curve, the time-dependent receiver operating characteristic (ROC) curve, and a nomogram were used to analyze the prognosis value of NKX2-1 for LUSC in terms of overall survival (OS) and progression-free survival (PFS)...
2024: PeerJ
#3
JOURNAL ARTICLE
Blanca Esther Blancas-Luciano, Ingeborg Becker-Fauser, Jaime Zamora-Chimal, Luis Jiménez-García, Reyna Lara-Martínez, Armando Pérez-Torres, Margarita González Del Pliego, Elsa Liliana Aguirre-Benítez, Ana María Fernández-Presas
BACKGROUND: Periodontitis is a chronic infectious disease, characterized by an exacerbated inflammatory response and a progressive loss of the supporting tissues of the teeth. Porphyromonas gingivalis is a key etiologic agent in periodontitis. Cystatin C is an antimicrobial salivary peptide that inhibits the growth of P. gingivalis . This study aimed to evaluate the antimicrobial activity of this peptide and its effect on cytokine production, nitric oxide (NO) release, reactive oxygen species (ROS) production, and programmed cell death in human macrophages infected with P...
2024: PeerJ
#4
JOURNAL ARTICLE
Francesca La Rosa, Ilaria Varotto-Boccazzi, Marina Saresella, Ivana Marventano, Giulia Maria Cattaneo, Ambra Hernis, Federica Piancone, Domenico Otranto, Sara Epis, Claudio Bandi, Mario Clerici
BACKGROUND: Innate immune responses against infectious agents can act as triggers of inflammatory diseases. On the other hand, various pathogens have developed mechanisms for the evasion of the immune response, based on an inhibition of innate immunity and inflammatory responses. Inflammatory diseases could thus be controlled through the administration of pathogens or pathogen-derived molecules, capable of interfering with the mechanisms at the basis of inflammation. In this framework, the NLRP3 inflammasome is an important component in innate antimicrobial responses and a major player in the inflammatory disease...
2024: Frontiers in Immunology
#5
JOURNAL ARTICLE
Yixin Chen, Ping Zhang, Fangwei Han, Yanying Zhou, Juexian Wei, Cailing Wang, Mingchuan Song, Shaopeng Lin, Yiming Xu, Xiaohui Chen
The enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoform 3 (PFKFB3) is a critical regulator of glycolysis and plays a key role in modulating the inflammatory response, thereby contributing to the development of inflammatory diseases such as sepsis. Despite its importance, the development of strategies to target PFKFB3 in the context of sepsis remains challenging. In this study, we employed a microRNA-based approach to decrease PFKFB3 expression. Through multiple meta-analyses, we observed a downregulation of miR-106a-5p expression and an upregulation of PFKFB3 expression in clinical sepsis samples...
May 3, 2024: Journal of Biological Chemistry
#6
JOURNAL ARTICLE
Chongjia C Chen, Kathleen M Buchheit, Pui Y Lee, Kailey E Brodeur, Aaqib Sohail, Laura Cho, Carolyn Baloh, Barbara Balestrieri, Mina Derakhshan, Chunli Feng, Joshua A Boyce, Daniel Dwyer, Tanya M Laidlaw
BACKGROUND: Aspirin-exacerbated respiratory disease (AERD) is a severe disease involving dysregulated type 2 inflammation. However, the role other inflammatory pathways play in AERD is poorly understood. OBJECTIVE: We sought to broadly define the inflammatory milieu of the upper respiratory tract in AERD and to determine the effects of IL-4Rα inhibition on mediators of nasal inflammation. METHODS: Twenty-two AERD patients treated with dupilumab for 3 months were followed over 3 visits and compared to 10 healthy controls...
May 2, 2024: Journal of Allergy and Clinical Immunology
#7
JOURNAL ARTICLE
Alessandra De Leo, Alessio Ugolini, Xiaoqing Yu, Fabio Scirocchi, Delia Scocozza, Barbara Peixoto, Angelica Pace, Luca D'Angelo, James K C Liu, Arnold B Etame, Aurelia Rughetti, Marianna Nuti, Antonio Santoro, Michael A Vogelbaum, Jose R Conejo-Garcia, Paulo C Rodriguez, Filippo Veglia
Immunosuppressive macrophages restrict anti-cancer immunity in glioblastoma (GBM). Here, we studied the contribution of microglia (MGs) and monocyte-derived macrophages (MDMs) to immunosuppression and mechanisms underlying their regulatory function. MDMs outnumbered MGs at late tumor stages and suppressed T cell activity. Molecular and functional analysis identified a population of glycolytic MDM expressing GLUT1 with potent immunosuppressive activity. GBM-derived factors promoted high glycolysis, lactate, and interleukin-10 (IL-10) production in MDMs...
April 30, 2024: Immunity
#8
JOURNAL ARTICLE
Shruti Rawal, Vinay Randhawa, Syed Husain Mustafa Rizvi, Madhur Sachan, Akm Khyrul Wara, Daniel Pérez-Cremades, Robert M Weisbrod, Naomi M Hamburg, Mark W Feinberg
AIMS: Diabetes leads to dysregulated macrophage immunometabolism, contributing to accelerated atherosclerosis progression. Identifying critical factors to restore metabolic alterations and promote resolution of inflammation remains an unmet goal. MicroRNAs (miRs) orchestrate multiple signaling events in macrophages, yet their therapeutic potential in diabetes-associated atherosclerosis remains unclear. METHODS AND RESULTS: MiRNA profiling revealed significantly lower miR-369-3p expression in aortic intimal lesions from Ldlr-/- mice on a high-fat sucrose containing (HFSC) diet for 12 weeks...
May 4, 2024: Cardiovascular Research
#9
REVIEW
Baishali Tamuli, Sakshi Sharma, Meena Patkar, Subir Biswas
BACKGROUND: The tumor microenvironment of solid tumors governs the differentiation of otherwise non-immunosuppressive macrophages and gamma delta (γδ) T cells into strong immunosuppressors while promoting suppressive abilities of known immunosuppressors such as myeloid-derived suppressor cells (MDSCs) upon infiltration into the tumor beds. RECENT FINDINGS: In epithelial malignancies, tumor-associated macrophages (TAMs), precursor monocytic MDSCs (M-MDSCs), and gamma delta (γδ) T cells often acquire strong immunosuppressive abilities that dampen spontaneous immune responses by tumor-infiltrating T cells and B lymphocytes against cancer...
May 2024: Cancer reports
#10
JOURNAL ARTICLE
Paul A De Sousa, Leo Perfect, Jinpei Ye, Kay Samuels, Ewa Piotrowska, Martin Gordon, Ryan Mate, Elsa Abranches, Thomas M Wishart, David H Dockrell, Aidan Courtney
BACKGROUND: Hyaluronan (HA) is an extracellular glycosaminoglycan polysaccharide with widespread roles throughout development and in healthy and neoplastic tissues. In pluripotent stem cell culture it can support both stem cell renewal and differentiation. However, responses to HA in culture are influenced by interaction with a range of cognate factors and receptors including components of blood serum supplements, which alter results. These may contribute to variation in cell batch production yield and phenotype as well as heighten the risks of adventitious pathogen transmission in the course of cell processing for therapeutic applications...
May 3, 2024: Stem Cell Research & Therapy
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JOURNAL ARTICLE
Ekaterina Pecksen, Sergey Tkachuk, Cristoph Schröder, Marc Vives Enrich, Anindita Neog, Cory P Johnson, Niko Lachmann, Hermann Haller, Yulia Kiyan
BACKGROUND: Induced pluripotent stem cells (iPSCs)-derived kidney organoids are a promising model for studying disease mechanisms and renal development. Despite several protocols having been developed, further improvements are needed to overcome existing limitations and enable a wider application of this model. One of the approaches to improve the differentiation of renal organoids in vitro is to include in the system cell types important for kidney organogenesis in vivo, such as macrophages...
May 3, 2024: Stem Cell Research & Therapy
#12
JOURNAL ARTICLE
Shilin Li, Xiang Li, Sishi Jiang, Chenglin Wang, Yingchun Hu
BACKGROUND: Sepsis ranks among the most formidable clinical challenges, characterized by exorbitant treatment costs and substantial demands on healthcare resources. Mitochondrial dysfunction emerges as a pivotal risk factor in the pathogenesis of sepsis, underscoring the imperative to identify mitochondrial-related biomarkers. Such biomarkers are crucial for enhancing the accuracy of sepsis diagnostics and prognostication. METHODS: In this study, adhering to the SEPSIS 3...
May 3, 2024: BMC Medical Genomics
#13
JOURNAL ARTICLE
A N Donlan, J L Leslie, M E Simpson, W A Petri, J E Allen, W A Petri
OBJECTIVES: Clostridioides difficile infection (CDI) is the leading hospital-acquired infection in North America. We have previously discovered that antibiotic disruption of the gut microbiota decreases intestinal IL-33 and IL-25 and increases susceptibility to CDI. We further found that IL-33 promotes protection through type 2 Innate Lymphoid Cells (ILC2s), which produce IL-13. However, the contribution of IL-13 to disease has never been explored. METHODS: We used a validated model of CDI in mice, in which we neutralized via blocking antibodies, or administered recombinant protein, IL-13 to assess the role of this cytokine during infection using weight and clinical scores...
May 1, 2024: Anaerobe
#14
JOURNAL ARTICLE
Mana Momenilandi, Romain Lévy, Steicy Sobrino, Jingwei Li, Chantal Lagresle-Peyrou, Hossein Esmaeilzadeh, Antoine Fayand, Corentin Le Floc'h, Antoine Guérin, Erika Della Mina, Debra Shearer, Ottavia M Delmonte, Ahmad Yatim, Kevin Mulder, Mathieu Mancini, Darawan Rinchai, Adeline Denis, Anna-Lena Neehus, Karla Balogh, Sarah Brendle, Hassan Rokni-Zadeh, Majid Changi-Ashtiani, Yoann Seeleuthner, Caroline Deswarte, Boris Bessot, Cassandre Cremades, Marie Materna, Axel Cederholm, Masato Ogishi, Quentin Philippot, Omer Beganovic, Mania Ackermann, Margareta Wuyts, Taushif Khan, Sébastien Fouéré, Florian Herms, Johan Chanal, Boaz Palterer, Julie Bruneau, Thierry J Molina, Stéphanie Leclerc-Mercier, Jean-Luc Prétet, Leila Youssefian, Hassan Vahidnezhad, Nima Parvaneh, Kristl G Claeys, Rik Schrijvers, Marine Luka, Philippe Pérot, Jacques Fourgeaud, Céline Nourrisson, Philippe Poirier, Emmanuelle Jouanguy, Stéphanie Boisson-Dupuis, Jacinta Bustamante, Luigi D Notarangelo, Neil Christensen, Nils Landegren, Laurent Abel, Nico Marr, Emmanuelle Six, David Langlais, Tim Waterboer, Florent Ginhoux, Cindy S Ma, Stuart G Tangye, Isabelle Meyts, Nico Lachmann, Jiafen Hu, Mohammad Shahrooei, Xavier Bossuyt, Jean-Laurent Casanova, Vivien Béziat
FMS-related tyrosine kinase 3 ligand (FLT3L), encoded by FLT3LG, is a hematopoietic factor essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs) in mice. We describe three humans homozygous for a loss-of-function FLT3LG variant with a history of various recurrent infections, including severe cutaneous warts. The patients' bone marrow (BM) was hypoplastic, with low levels of hematopoietic progenitors, particularly myeloid and B cell precursors. Counts of B cells, monocytes, and DCs were low in the patients' blood, whereas the other blood subsets, including NK cells, were affected only moderately, if at all...
May 2, 2024: Cell
#15
JOURNAL ARTICLE
Xin Guan, Sitong Zhu, Jinqian Song, Kui Liu, Mei Liu, Luyang Xie, Yifang Wang, Jin Wu, Xiaojun Xu, Tao Pang
Neuroinflammation plays a significant role in ischemic injury, which can be promoted by oxidized mitochondrial DNA (Ox-mtDNA). Cytidine/uridine monophosphate kinase 2 (CMPK2) regulates mtDNA replication, but its role in neuroinflammation and ischemic injury remains unknown. Here, we report that CMPK2 expression is upregulated in monocytes/macrophages and microglia post-stroke in humans and mice, respectively. Microglia/macrophage CMPK2 knockdown using the Cre recombination-dependent adeno-associated virus suppresses the inflammatory responses in the brain, reduces infarcts, and improves neurological outcomes in ischemic CX3 CR1Cre/ERT2 mice...
May 2, 2024: Cell reports medicine
#16
JOURNAL ARTICLE
Shifaa Abdin, Friederike Mansel, Anna Hashtchin, Mania Ackermann, Gesine Hansen, Bjoern Becker, Benjamin Kick, Nhi Pham, Hendrik Dietz, Christoph Schaniel, Ulrich Martin, Ingo Spreitzer, Nico Lachmann
Ensuring the safety of parenteral drugs before injection into patients is of utmost importance. New regulations around the globe and the need to refrain from using animals however, have highlighted the need for new cell sources to be used in next-generation bioassays to detect the entire spectrum of possible contaminating pyrogens. Given the current drawbacks of the Monocyte-Activation-Test (MAT) with respect to the use of primary peripheral blood mono-nuclear cells (PBMCs) or the use of monocytic cell lines, we here demonstrate the manufacturing of sensor monocyte/macrophages from human induced pluripotent stem cells (iMonoMac), which are fully defined and superior to current cell products...
May 3, 2024: Biofabrication
#17
JOURNAL ARTICLE
Monali Praharaj, Fan Shen, Alex J Lee, Liang Zhao, Thomas R Nirschl, Debebe Theodros, Alok K Singh, Xiaoxu Wang, Kenneth M Adusei, Kara A Lombardo, Raekwon A Williams, Laura A Sena, Elizabeth A Thompson, Ada Tam, Srinivasan Yegnasubramanian, Edward J Pearce, Robert D Leone, Jesse Alt, Rana Rais, Barbara S Slusher, Drew M Pardoll, Jonathan D Powell, Jelani C Zarif
Glutamine metabolism in tumor microenvironments critically regulates anti-tumor immunity. Using glutamine-antagonist prodrug JHU083, we report potent tumor growth inhibition in urologic tumors by JHU083-reprogrammed tumor-associated macrophages (TAMs) and tumor-infiltrating monocytes (TIMs). We show JHU083-mediated glutamine antagonism in tumor microenvironments induces TNF, pro-inflammatory, and mTORC1 signaling in intratumoral TAM clusters. JHU083-reprogrammed TAMs also exhibit increased tumor cell phagocytosis and diminished pro-angiogenic capacities...
May 3, 2024: Cancer Immunology Research
#18
JOURNAL ARTICLE
Christophe Huret, Léa Ferrayé, Antoine David, Myriame Mohamed, Nicolas Valentin, Frédéric Charlotte, Magali Savignac, Michele Goodhardt, Jean-Charles Guéry, Claire Rougeulle, Céline Morey
In mammals, males and females show marked differences in immune responses. Males are globally more sensitive to infectious diseases, while females are more susceptible to systemic autoimmunity. X-chromosome inactivation (XCI), the epigenetic mechanism ensuring the silencing of one X in females, may participate in these sex biases. We perturbed the expression of the trigger of XCI, the noncoding RNA Xist , in female mice. This resulted in reactivation of genes on the inactive X, including members of the Toll-like receptor 7 (TLR7) signaling pathway, in monocyte/macrophages and dendritic and B cells...
May 3, 2024: Science Advances
#19
JOURNAL ARTICLE
Weihao Zheng, I-Chang Chang, Jason Limberis, Jonathan M Budzik, Beth Shoshana Zha, Zachary Howard, Lucas Chen, Joel D Ernst
Mycobacterium tuberculosis (Mtb) infects lung myeloid cells, but the specific Mtb-permissive cells and host mechanisms supporting Mtb persistence during chronic infection are incompletely characterized. We report that after the development of T cell responses, CD11clo monocyte-derived cells harbor more live Mtb than alveolar macrophages (AM), neutrophils, and CD11chi monocyte-derived cells. Transcriptomic and functional studies revealed that the lysosome pathway is underexpressed in this highly permissive subset, characterized by less lysosome content, acidification, and proteolytic activity than AM, along with less nuclear TFEB, a regulator of lysosome biogenesis...
May 3, 2024: PLoS Pathogens
#20
JOURNAL ARTICLE
Hong-Bin Lin, Pu Hong, Meng-Yu Yin, Zhi-Jun Yao, Jin-Yu Zhang, Yan-Pin Jiang, Xuan-Xuan Huang, Shi-Yuan Xu, Feng-Xian Li, Hong-Fei Zhang
BACKGROUND: Cardiac damage induced by ischemic stroke, such as arrhythmia, cardiac dysfunction, and even cardiac arrest, is referred to as cerebral-cardiac syndrome (CCS). Cardiac macrophages are reported to be closely associated with stroke-induced cardiac damage. However, the role of macrophage subsets in CCS is still unclear due to their heterogeneity. Sympathetic nerves play a significant role in regulating macrophages in cardiovascular disease. However, the role of macrophage subsets and sympathetic nerves in CCS is still unclear...
May 3, 2024: Journal of the American Heart Association
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