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Mohammad Shboul, Paul Roschger, Rudolf Ganger, Lefteris Paschalis, Stamatia Rokidi, Shahin Zandieh, Jana Behunova, Christian Muschitz, Astrid Fahrleitner-Pammer, Alvin Yu Jin Ng, Sumanty Tohari, Byrappa Venkatesh, Carine Bonnard, Bruno Reversade, Klaus Klaushofer, Ali Al Kaissi
Analysis of tissue from a 34-years-old male patient from Austrian origin with a history of multiple fractures associated with painful episodes over the carpal, tarsal and at the end of the long bones respectively is presented. Radiographic images and axial 3DCT scans showed widespread defects in trabecular bone architecture and ill-defined cortices over these skeletal sites in the form of discrete cystic-like lesions. Family history indicated two sisters (one half and one full biological sisters) also with a history of fractures...
October 10, 2018: Bone
Li-Ping He, Xing-Sheng Zhao, Le-Ping He
Background The prevalence of coronary heart disease (CHD) appears to be high among Chinese Mongolians. MiR-23b has been proven to play a key role in atherosclerosis. The expression and role of miR-23b in the Mongolians at high cardiovascular risk were explored in this study. Methods 40 cases of blood samples from the Mongolians at high cardiovascular risk were enrolled in this study. The expression of miR-23b was quantified by qRT-PCR. To induce monocytes differentiation into macrophages HP-1 cells were cultured with phorbol 12-myristate 13-acetate (PMA)...
October 12, 2018: Bioscience Reports
Pawel Karpinski, Pawel Skiba, Magdalena Kosinska, Marta Rosiek-Biegus, Emilia Królewicz, Nikolaus Blin, Eckart Meese, Bernard Panaszek, Marita Nittner-Marszalska, Maria Malgorzata Sasiadek
BACKGROUND: Insect venom immunotherapy (VIT) is used to protect patients against Hymenoptera insects' venom allergy (HVA), which can result in severe systemic or even life-threatening conditions. Molecular mechanisms triggered by VIT remain largely unknown. OBJECTIVE: To compare genome-wide gene expression of patients with severe HVA prior to VIT and 12 months after. METHODS: Whole blood RNA samples were analyzed on an expression array. Results from differential expression obtained on a microarray platform were confirmed by quantitative real -time PCR (qRT-PCR)...
October 9, 2018: Immunology Letters
Shao-Jie Chen, Guo-da Lian, Jia-Jia Li, Qiu-Bo Zhang, Lin-Juan Zeng, Ke-Ge Yang, Chu-Mei Huang, Ya-Qing Li, Yin-Ting Chen, Kai-Hong Huang
Tumor-associated macrophages (TAMs) are abundant population of inflammatory cells which play an essential role in remodeling tumor microenvironment and tumor progression. Previously, we found the high density of TAMs was correlated with lymph node metastasis and poor prognosis in pancreatic ductal adenocarcinoma (PDAC). Therefore, this study was designed to investigate the mechanisms of interaction between TAMs and PDAC. THP-1 monocytes were the exposure to conditioned media (CM) produced by PDAC cells; then, monocyte recruitment and macrophage differentiation were assessed...
October 12, 2018: Cancer Medicine
Naike Casagrande, Cinzia Borghese, Lydia Visser, Maurizio Mongiat, Alfonso Colombatti, Donatella Aldinucci
Classic Hodgkin lymphoma tumor cells express a functional CCR5 receptor, and tumor tissues express high CCL5 levels, suggesting that CCL5-CCR5 signaling is involved in tumor-microenvironment formation and tumor growth. Using the CCR5 antagonist maraviroc and a neutralizing anti-CCL5 antibody, we found that CCL5 secreted by Classic Hodgkin lymphoma cells recruited Mesenchymal-stromal cells and monocytes. Education of Mesenchymal-stromal cells by tumor cell conditioned medium enhanced Mesenchymal-stromal cells proliferation and CCL5 secretion...
October 11, 2018: Haematologica
Zong-Yuan Jiang, Jun-Jian Jiang, Yu-Shui Ma, Hong-Ye Li, Wei Shi, Pei-Liang Fu, Cong-Feng Xu, Jiu-Zhou Lu, Da Fu, Jian-Guang Xu
Giant-cell tumor (GCT) of the bone is an invasiveness and high recurrent bone tumor that is considered borderline or potentially malignant. To explore the molecular mechanism leading to bone destruction and identify novel targets for treatment, we conducted silencing of miR-223 and miR-19a in stromal giant cells and identified TWIST and Runx2 as their target genes. We investigated the impact of these microRNAs and their target genes on stromal giant cells that promote the differentiation of monocyte/macrophages into osteoclast cells and recruitment to the bone microenvironment, which in turn enhances the bone destruction capacity of GCT...
October 8, 2018: Biochemical and Biophysical Research Communications
Caterina Peña, Renata Vargas, Juan Pablo Hernandez-Fonseca, Jesús Mosquera
The up-regulation of HLA antigens is important during heart inflammatory events and myofibroblasts may modulate the expression of this molecule in tissues. To test this possibility, the effect of cardiac myofibroblast:macrophage contact and the production of myofibroblast inhibitor factor(s) on the macrophage HLA (Ia) expression were studied. Listeria monocytogenes-elicited Ia + peritoneal macrophages (high Ia expression) were co-cultured with cardiac myofibroblasts for 3 and 7 days (myofibroblast contact)...
October 2018: Tissue & Cell
Kan Hong, Zhiming Yu, Xueqian Sun, Chen Wu, Ping Weng, Mingxia Wei, Jing Zuo, Junliang Chen, Qingfeng Pang
OBJECTIVE: To investigate the effect of caveolin-1 scaffolding domain (CSD) peptides on heme oxygenase-1 (HO-1) activity increasing and M1/M2 phenotype polarization in rat alveolar macrophages (AMs) induced by lipopolysaccharide (LPS). METHODS: Bioinformatics was used to analyze the binding of full-length wild-type CSD polypeptide and 101 amino acid deleted truncated mutant CSD polypeptide (Δ101CSD) to HO-1. Primary AMs were isolated from rats, when cell fusion reached 80%, they were synchronized with serum-free medium and divided into five groups: no treatment was given to the blank control group; LPS group was treated with 100 μg/L LPS for 16 hours; LPS + hemin group was treated with 100 μg/L LPS and 20 μmol/L hemin for 16 hours; wild-type CSD polypeptide + LPS + hemin group was pretreated with 10 μmol/L wild-type CSD polypeptide 6 hours before LPS treatment; Δ101CSD + LPS + hemin group was pretreated with 10 μmol/L Δ101CSD polypeptide 6 hours before LPS treatment...
September 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
Stefan Wallner, Evelyn Orsó, Margot Grandl, Tatiana Konovalova, Gerhard Liebisch, Gerd Schmitz
BACKGROUND: Plasmalogens are either phosphatidylcholine (PC P) or phosphatidylethanolamine (PE P) glycerophospholipids containing a vinyl ether moiety in sn-1-position and an esterified fatty acid in sn-2 position. Multiple functions have been proposed, including reservoir of precursors for inflammatory mediators, modulation of membrane fluidity, and anti-oxidative properties. They could therefore play a role under conditions of metabolic stress. Especially enzymatically modified LDL (eLDL) and oxidatively modified LDL (oxLDL) represent modifications of LDL that are taken up by macrophages in atherosclerotic plaques...
2018: PloS One
Anne Schink, Jan Neumann, Anna Lena Leifke, Kira Ziegler, Janine Fröhlich-Nowoisky, Christoph Cremer, Eckhard Thines, Bettina Weber, Ulrich Pöschl, Detlef Schuppan, Kurt Lucas
Herbal extracts represent an ample source of natural compounds, with potential to be used in improving human health. There is a growing interest in using natural extracts as possible new treatment strategies for inflammatory diseases. We therefore aimed at identifying herbal extracts that affect inflammatory signaling pathways through toll-like receptors (TLRs), TLR2 and TLR4. Ninety-nine ethanolic extracts were screened in THP-1 monocytes and HeLa-TLR4 transfected reporter cells for their effects on stimulated TLR2 and TLR4 signaling pathways...
2018: PloS One
Joanne L Tuohy, Jonathan E Fogle, Kristina Meichner, Luke B Borst, Christopher S Petty, Emily H Griffith, Jason A Osborne, B Duncan X Lascelles
OBJECTIVE: To evaluate the effects of nanoparticle hyperthermia therapy on monocyte function and tumor-derived factors associated with macrophage polarization in a murine osteosarcoma model. STUDY DESIGN: Experimental study. ANIMALS: Female C3H mice. METHODS: Peripheral blood monocyte cell surface phenotype, monocyte chemotaxis, tumor messenger RNA expression, and survival were compared among osteosarcoma (OS)-bearing mice treated with nanoparticle hyperthermia therapy, OS-bearing mice with osteomyelitis, OS-bearing mice, vehicle control mice, and normal control mice...
October 11, 2018: Veterinary Surgery: VS
Omar Vélez López, Santhi Gorantla, Annabell C Segarra, María C Andino Norat, Manuel Álvarez, Richard L Skolasky, Loyda M Meléndez
Pathogenesis of HIV-associated neurocognitive disorders (HAND) is mediated through the infiltration of perivascular macrophages into the brain with the secretion of viral, neurotoxic and inflammatory proteins. One of these proteins is cathepsin B (CATB), a lysosomal cysteine protease that induces neuronal apoptosis, and increases in plasma and cerebrospinal fluid from HIV-1 infected patients (Cantres-Rosario et al. AIDS 27(3):347-356, 2013). Cocaine further potentiates CATB neurotoxicity in vitro and in vivo (Zenón et al...
October 10, 2018: Journal of Neuroimmune Pharmacology: the Official Journal of the Society on NeuroImmune Pharmacology
Young-Sun Lee, Myung-Ho Kim, Hyon-Seung Yi, So Yeon Kim, Hee-Hoon Kim, Ji Hoon Kim, Jong Eun Yeon, Kwan Soo Byun, Jin-Seok Byun, Won-Il Jeong
The expression of chemokine receptor CX3 CR1 is related to migration and signaling in cells of the monocyte-macrophage lineage. The precise roles of CX3 CR1 in the liver have been investigated but not clearly elucidated. Here, we investigated the roles of CX3 CR1 in hepatic macrophages and liver injury. Hepatic and splenic CX3 CR1low F4/80low monocytes and CX3 CR1low CD16- monocytes were differentiated into CX3 CR1high F4/80high or CX3 CR1high CD16+ macrophages by co-culture with endothelial cells. Moreover, CX3 CL1 deficiency in human umbilical vein endothelial cells (HUVECs) attenuated the expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α), whereas recombinant CX3 CL1 treatment reversed this expression in co-cultured monocytes...
October 10, 2018: Scientific Reports
Hiroshi Fujiwara
In cancer immunotherapy, the importance of tumor microenvironment (TME) has recently been highlighted. TME, where trafficked T lymphocytes with tumoricidal activity, particularly endowed by dendritic cells in the sentinel lymph nodes, suppress cancer cells, consists of multiple cellular components, such as fibroblasts, macrophages, myeloid-derived suppressor cells, monocytes, neutrophils, regulatory T cells, and NK cells, and non-cellular components, such as extracellular matrix and blood and lymphatic vessels...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Daigo Hashimoto
Emerging evidence has revealed that tissue-resident macrophages (TRMs) are differentiated from fetal precursors, including yolk sac macrophages, and late erythro-myeloid progenitors (EMPs) derived from fetal monocytes. Furthermore, TRMs can locally undergo self-renewal independent of circulating bone marrow-derived monocytes. Recent data has indicated the existence of a TRM niche that controls TRM differentiation, maintenance, and diversification. Niche availability and accessibility for different TRM precursors determine the cell origin of each TRM...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Mireya Becerra-Díaz, Ashley B Strickland, Aleksander Keselman, Nicola M Heller
Allergic asthma is a disease initiated by a breach of the lung mucosal barrier and an inappropriate Th2 inflammatory immune response that results in M2 polarization of alveolar macrophages (AM). The number of M2 macrophages in the airway correlates with asthma severity in humans. Sex differences in asthma suggest that sex hormones modify lung inflammation and macrophage polarization. Asthmatic women have more M2 macrophages than asthmatic men and androgens have been used as an experimental asthma treatment...
October 10, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Xia Liu, Cong Li, Jie Zhu, Wenshuai Li, Qiang Zhu
BACKGROUND: Accumulating evidence has suggested the involvement of miR-545 and long noncoding RNA (lncRNA) FTX in a wide range of diseases. Therefore, this study aimed to investigate the molecular mechanism underlying the function of miR-545 and lncRNA FTX in hepatitis B virus (HBV)-related cirrhosis. METHOD: The level of Tim-3, TLR-4, and endotoxin was detected in CD14+ , CD14 + CD16 + , and CD14 + CD16 - monocytes isolated from both patients with cirrhosis and healthy controls...
October 10, 2018: Journal of Cellular Biochemistry
Agnieszka Nikołajuk, Natalia Matulewicz, Magdalena Stefanowicz, Monika Karczewska-Kupczewska
Insulin resistance increases the risk for cardiovascular disease (CVD) even in the absence of classic risk factors, such as hyperglycemia, hypertension, dyslipidemia, and obesity. Low-grade chronic inflammatory state is associated both with insulin resistance and atherosclerosis. An increased circulating level of proinflammatory proatherogenic factors and biomarkers of endothelial activation was observed in diabetes and CVD. The aim of our study was to assess serum proatherogenic and proinflammatory factors in young healthy nonobese subjects with positive family history of type 2 diabetes...
2018: International Journal of Endocrinology
Roel Tans, Janna A van Diepen, Sabina Bijlsma, Lars Verschuren, Anouk Suppers, Rinke Stienstra, Ron A Wevers, Cees J Tack, Jolein Gloerich, Alain J van Gool
BACKGROUND: Overweight and obesity can lead to adipose tissue inflammation, which causes insulin resistance and on the long-term type 2 diabetes mellitus (T2D). The inflammatory changes of obese-adipose tissue are characterized by macrophage infiltration and activation, but validated circulating biomarkers for adipose tissue inflammation for clinical use are still lacking. One of the most secreted enzymes by activated macrophages is chitotriosidase (CHIT1). OBJECTIVE: To test whether circulating CHIT1 enzymatic activity levels reflect adipose tissue inflammation...
October 9, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
Xiu-Hao Guan, Yu-Xiao Hong, Rui-Qun Qi, Xing-Hua Gao
Candida albicans (C. albicans) is an opportunistic human fungal pathogen that colonises the skin. Both keratinocytes and macrophages play crucial roles in host defence against C. albicans. However, the interaction of keratinocytes with macrophages during C. albicans colonisation has not been well studied. In this study, macrophages were cultured in conditioned medium from keratinocytes treated with heat-inactivated C. albicans (CM-C. albicans), macrophage migration and polarised activation and were then assessed by a Transwell assay, flow cytometry, quantitative real-time PCR (qPCR), Western blot and an enzyme-linked immunosorbent assay (ELISA)...
September 30, 2018: Cellular and Molecular Biology
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