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monocytes macrophages

Lukas R H Gerken, Kerda Keevend, Yucheng Zhang, Fabian H L Starsich, Christian Eberhardt, Guido Panzarasa, Martin T Matter, Adrian Wichser, Andreas Boss, Antonia Neels, Inge K Herrmann
High-Z metal oxide nanoparticles hold promise as imaging probes and radio-enhancers. Hafnium dioxide nanoparticles have recently entered clinical evaluation. Despite promising early clinical findings, the potential of HfO2 as a matrix for multimodal theranostics is yet to be developed. Here, we investigate the physicochemical properties and the potential of HfO2-based nanoparticles for multimodal theranostic imaging. Undoped and lanthanide (Eu3+, Tb3+, and Gd3+)-doped HfO2 nanoparticles were synthesized and functionalized with various moieties, including poly(vinylpyrrolidone) (PVP), (3-aminopropyl)triethoxysilane (APTES) and folic acid (FA)...
December 5, 2018: ACS Applied Materials & Interfaces
Zhen Zhang, Hao Wen, Xiaobin Yang, Ke Zhang, Baorong He, Xinliang Zhang, Lingbo Kong
Bone homeostasis is strictly regulated by balanced activity of bone-forming osteoblasts and bone-resorbing osteoclasts.Disruption of the balance of activity between osteoblasts and osteoclasts leads to various metabolic bone diseases.Osteoclasts are cells of hematopoietic origin that they are large, multinucleated cells formed by the fusion of precursor cells of monocyte/macrophage lineage,they are unique cells that degrade the bone matrix. activation of transcription factors nuclear factor-activated T cells c1 (NFATc1) is required for sufficient osteoclast differentiation and it plays the role of a master transcription regulator of osteoclast differentiation,meanwhile,NFATc1 could be employed to elicit anabolic effects on bone...
December 5, 2018: Current Stem Cell Research & Therapy
Kinga Musiał, Danuta Zwolińska
CONTEXT: Chronic kidney disease (CKD) is characterized by immunocompetent cell migration and inflammation. Monocyte chemoattractant protein (MCP)-1 and macrophage colony-stimulating factor (MCSF) stimulate monocyte migration and transition into macrophages, with subsequent release of neopterin. OBJECTIVE: The aim of the study was to analyze these parameters in children with various stages of CKD. MATERIAL AND METHODS: The study group consisted of 41 CKD children, 19 patients on hemodialysis (HD), 22 children on automated peritoneal dialysis (APD), and 23 controls...
December 5, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Lisa K Kozicky, Susan C Menzies, Zheng Yu Zhao, Tariq Vira, Kiera Harnden, Kwestan Safari, Kate L Del Bel, Stuart E Turvey, Laura M Sly
Intravenous Immunoglobulin (IVIg) is used to treat autoimmune or inflammatory diseases, but its mechanism of action is not completely understood. We asked whether IVIg can induce interleukin-10 (IL-10) and reduce pro-inflammatory cytokine production in human monocytes, and whether this response is reduced in monocytes from people with an Fcγ receptor IIA (FcγRIIA) gene variant, which is associated with increased risk of inflammatory diseases and poor response to antibody-based biological therapy. IVIg increased IL-10 production and reduced pro-inflammatory cytokine production in response to bacterial lipopolysaccharide (LPS), which required FcγRI and FcγRIIB and activation of MAPKs, extracellular signal-regulated kinase 1/2 (ERK1/2), and p38...
2018: Frontiers in Immunology
Paul Lohan, Nick Murphy, Oliver Treacy, Kevin Lynch, Maurice Morcos, Bingling Chen, Aideen E Ryan, Matthew D Griffin, Thomas Ritter
High-risk cornea transplant recipients represent a patient population with significant un-met medical need for more effective therapies to prevent immunological graft rejection due to heightened anti-donor immune response. In this study, a rat model of pre-existing anti-donor immunity was developed in which corneal allografts were rejected earlier than in non-pre-sensitized recipients. In this model, third-party (non-donor, non-recipient strain) allogeneic mesenchymal stromal cells (allo-MSC) were administered intravenously 7 and 1 days prior to transplantation...
2018: Frontiers in Immunology
Satoshi Hara, Soichiro Fukumura, Hiroshi Ichinose
6-Pyruvoyl tetrahydropterin synthase (PTS) converts 7, 8-dihydroneopterin triphosphate into 6-pyruvoyltetrahydropterin and is a critical enzyme for the de novo synthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. Neopterin derived from 7,8-dihydroneopterin triphosphate is secreted by monocytes/macrophages, and is a well-known biomarker for cellular immunity. Because PTS activity in the cell can be a determinant of neopterin production, here we used recombinant human PTS protein to investigate how its activity is regulated, especially depending on redox conditions...
December 4, 2018: Journal of Biological Chemistry
Brad W Porter, Thara K Venkatappa
Latent tuberculosis infection (LTBI) is diagnosed immunologically using the Mantoux tuberculin skin test (TST) or interferon-gamma release assays (IGRAs). While widely used, immunodiagnostics can produce false negative or false positive results. Pathogen biomarkers provide an alternative, but direct detection in LTBI and extrapulmonary TB cases is challenging. Mycobacterium tuberculosis grows slowly, has limited hematogenous movement, is protected by a lipid rich cell wall, and produces low levels of secreted factors...
December 2018: Tuberculosis
Yutao Liu, Yuan Gao, Jing Yang, Changhe Shi, Yanlin Wang, Yuming Xu
BACKGROUND: Idiopathic inflammatory myopathies (IIM) are a group of autoimmune diseases characterized by muscle disorders. We conducted this study to detect whether NF-E2-related factor 2 (Nrf2) pathway inhibit inflammatory infiltration by macrophage in experimental autoimmune myositis (EAM) rat model. METHODS: CD163 levels were examined by immunohistochemistry (IHC), while serum creatine kinase (CK), reactive oxygen species (ROS), and serum monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) levels were determined by enzyme linked immunosorbnent assay (ELISA), both in IIM patients and EAM rat...
December 1, 2018: Molecular Immunology
Yingjia Chen, Tai L Guo
SCOPE: Glycation products are ubiquitous in food at high concentrations in the Western diet. The well-controlled glycation resulting in the production of early glycation products (EGPs) has been proposed as a strategy to improve the physicochemical properties of food proteins. However, the health effects of EGPs are unknown. It has been shown that the Western diet (glycation prone) is associated with a higher mortality in prostate cancer (PCa) patients than the prudent diet; therefore, we have investigated the role of EGPs in prostate tumorigenesis...
December 4, 2018: Molecular Nutrition & Food Research
Shengnan Wang, Wang Lyu, Suxian Lin, Yang Lu, Jianchang Jiang, Xiaochun Zhu
OBJECTIVE: To investigate the effect of vitamin D3 on polarization of monocyte macrophages induced by serum from patients with ankylosing spondylitis (AS). METHODS: Twenty AS naïve patients and 20 healthy controls from Wenzhou People's Hospital during January 2016 and December 2017 were enrolled. The macrophages were differentiated from THP1 cells induced by phorbol 12-myristate 13-acetate (PMA), and then co-cultured with the serum from healthy subjects (control group) or AS patients...
February 25, 2018: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Chao Huang, Jian Wang, Xiaodong Zheng, Yongyan Chen, Haiming Wei, Rui Sun, Zhigang Tian
The activation of Toll-like receptor (TLR) signaling is widely reported to be involved in preventing the development of allergic asthma. However, the mechanism of the protective function of TLR signaling remains limited. Here, we studied the mouse model of ovalbumin (OVA)-induced allergic asthma and found that deficiency of TLR signaling or activating TLR signaling with agonist would aggravate or attenuate OVA-induced allergic asthma, respectively, and TLR signaling-mediated protective effect mainly affected the sensitization phase...
2018: Frontiers in Immunology
Yu Yang, Zheng-Kun Tu, Xing-Kai Liu, Ping Zhang
The mononuclear phagocyte system (MPS), which consists of monocytes, dendritic cells (DCs), and macrophages, plays a vital role in the innate immune defense against pathogens. Hepatitis C virus (HCV) is efficient in evading the host immunity, thereby facilitating its development into chronic infection. Chronic HCV infection is the leading cause of end-stage liver diseases, liver cirrhosis, and hepatocellular carcinoma. Acquired immune response was regarded as the key factor to eradicate HCV. However, innate immunity can regulate the acquired immune response...
November 28, 2018: World Journal of Gastroenterology: WJG
Sho Shibata, Kensuke Miyake, Tomoya Tateishi, Soichiro Yoshikawa, Yoshinori Yamanishi, Yasunari Miyazaki, Naohiko Inase, Hajime Karasuyama
Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. It has generally been considered a non-Th2-type lung disorder, characterized by progressive airflow limitation with inflammation and emphysema, but its cellular and molecular mechanism remains ill defined, compared with that of asthma characterized by reversible airway obstruction. Here we show a previously unappreciated role for basophils at the initiation phase of emphysema formation in an elastase-induced murine model of COPD in that basophils represent less than 1% of lung-infiltrating cells...
December 3, 2018: Proceedings of the National Academy of Sciences of the United States of America
Michael J Wiegand, Gregory W Kubacki, Jeremy L Gilbert
Electrochemical interactions at the cell-metal interface determine cell viability and influence behavior in response to different electrode potential conditions, specifically cathodic biases. Mechanically assisted crevice corrosion, for example, induces cathodic potentials and the associated electrochemical consequences of increased reduction reactions at the implant surface may affect cell viability in a manner that is different for various cell phenotypes. Monocyte macrophage-like U937 cells were cultured on cobalt-chromium-molybdenum (CoCrMo) metal surfaces in vitro for 24 h to assess cell behavior in response to sustained applied voltages...
December 3, 2018: Journal of Biomedical Materials Research. Part A
Ge Li, Chunmei Hou, Shuaijie Dou, Jiacheng Zhang, Yanling Zhang, Yiqiong Liu, Zhiding Wang, He Xiao, Renxi Wang, Guojiang Chen, Yan Li, Jiannan Feng, Beifen Shen, Gencheng Han
T cell immunoglobulin and mucin domain protein 3 (Tim-3) is an immune checkpoint inhibitor in T cells and innate immune cells. The deregulated upregulation of Tim-3 is related to immune exhaustion in tumor and viral infection. To overcome Tim-3-mediated immune tolerance, we developed a novel monoclonal antibody against human Tim-3 (L3G) and investigated its roles in inhibiting Tim-3 signaling and overcoming immune tolerance in T cells and monocytes/macrophages. The administration of L3G to cultured peripheral blood mononuclear cells (PBMCs) significantly increased the production of IFN-γ and IL-2 and the expression of type I interferon...
December 1, 2018: Scandinavian Journal of Immunology
Yun Wu, Wanyue Xu, Jingya Hou, Yanqing Liu, Rong Li, Jingbo Liu, Chen Li, Xiaolin Tang, Li Lin, Yaping Pan, Dongmei Zhang
Porphyromonas gingivalis (P. gingivalis) is an important pathogen that contributes to periodontal disease and causes infections that promote the progression of atherosclerosis. Our previous studies showed that macrophage migration inhibitory factor (MIF) facilitates monocyte adhesion to endothelial cells by regulating the expression of intercellular adhesion molecule-1 (ICAM-1) in P. gingivalis-infected endothelial cells. However, the detailed pathological role of MIF has yet to be elucidated in this context...
December 3, 2018: Inflammation
Pablo Ariel Isidoro Slullitel, Rodrigo Brandariz, Jose Ignacio Oñativia, German Farfalli, Fernando Comba, Francisco Piccaluga, Martin Buttaro
PURPOSE: It has been acknowledged that implant wear correlates with the risk for periprosthetic osteolysis, being aggressive granulomatosis the worst expression of bone resorption. We sought to determine the clinical, radiological, and histological features of aggressive granulomatosis after primary total hip arthroplasty (THA). METHODS: We included nine cases with aggressive granulomatosis of the hip around cemented stems. Indications for revision THA consisted of progressive signs of extensive bone resorption or implant loosening...
November 30, 2018: International Orthopaedics
Yan Cheng, Xinying Tang, Yixuan Li, Dandan Zhao, Qiuhua Cao, Hongxi Wu, Hongbao Yang, Kun Hao, Yong Yang
PURPOSE: Psychological depression has been shown to dysregulate the immune system and promote tumor progression. The aim of this study is to investigate how psychological depression alters the immune profiles in prostate cancer. EXPERIMENTAL DESIGN: We used a murine model of depression in Myc-CaP tumor bearing immune competent FVB mice and Hi-myc mice presenting with spontaneous prostate cancer. Transwell migration and co-culture assays were used to evaluate myeloid cell trafficking and cytokine profile changes evoked by Myc-CaP cells that had been treated with norepinephrine (NE), a major elevated neurotransmitter in depression...
November 30, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
David P Steensma
Clonally restricted hematopoiesis is a common aging-associated biological state that predisposes to subsequent development of a hematological malignancy or cardiovascular death. Clonal expansion driven by leukemia-associated somatic mutations, such as DNMT3A , ASXL1 , or TET2 , is best characterized, but oligoclonality can also emerge without recognized leukemia-driver mutations, perhaps as a result of stochastic neutral drift. Murine models provide compelling evidence that a major mechanism of increased cardiovascular mortality in the context of clonal hematopoiesis is accelerated atherogenesis driven by inflammasome-mediated endothelial injury, resulting from proinflammatory interactions between endothelium and macrophages derived from circulating clonal monocytes...
November 30, 2018: Hematology—the Education Program of the American Society of Hematology
Zsolt Regdon, Agnieszka Robaszkiewicz, Katalin Kovács, Żaneta Rygielska, Csaba Hegedűs, Khaldon Bodoor, Éva Szabó, László Virág
In inflamed tissues or during ischemia-reperfusion episodes, activated macrophages produce large amounts of reactive species and are, thus, exposed to the damaging effects of reactive species. Here, our goal was to investigate the mechanism whereby activated macrophages protect themselves from oxidant stress-induced cell death. Hydrogen peroxide-treated mouse bone marrow-derived macrophages (BMDM) and THP-1 human monocyte-derived cells were chosen as models. We found a gradual development of resistance: first in monocyte-to-macrophage differentiation, and subsequently after lipopolysaccharide (LPS) exposure...
November 28, 2018: Free Radical Biology & Medicine
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