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https://www.readbyqxmd.com/read/30516370/injectable-magnetically-orienting-electrospun-fiber-conduits-for-neuron-guidance
#1
Christopher D L Johnson, Debmalya Ganguly, Jonathan M Zuidema, Thomas J Cardinal, Alexis M Ziemba, Kathryn R Kearns, Simon M McCarthy, Deanna M Thompson, Ganpati Ramanath, Diana-Andra Borca-Tasciuc, Silvio Dutz, Ryan J Gilbert
Magnetic electrospun fibers are of interest to biomaterial applications that strive to provide cell guidance that can be delivered using minimally invasive techniques. Magnetic electrospun fibers can be injected then magnetically positioned in situ, and the aligned fiber scaffolds provide consistent topographical guidance to cells. We developed and tested magnetically-responsive aligned poly-L-lactic acid (PLLA) electrospun fiber scaffolds for neural applications. Incorporating oleic-acid-coated iron oxide nanoparticles significantly increased neurite outgrowth, reduced the fiber alignment, and increased the surface nanotopography of the electrospun fibers...
December 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30515676/precision-cut-liver-slices-a-versatile-tool-to-advance-liver-research
#2
REVIEW
Elena Palma, Ewald Jan Doornebal, Shilpa Chokshi
Human precision-cut liver slices represent a robust and versatile ex vivo model which retains the complex and multi-cellular histoarchitecture of the hepatic environment. As such, they represent an ideal model to investigate the mechanisms of liver injury and for the identification of novel therapeutic targets. Schematic overview to highlight the utility of precision-cut liver slices as a relevant and versatile ex-vivo model of liver disease. Top panel; Precision cut liver slices (PCLS) exposed to ethanol develop mega-mitochondria, a classical hallmark of Alcoholic Liver Disease (ALD)...
December 4, 2018: Hepatology International
https://www.readbyqxmd.com/read/30514673/-effects-of-sera-of-rats-fed-with-huganqingzhi-tablets-on-endoplasmic-reticulum-stress-in-a-hepg2-cell-model-of-nonalcoholic-fatty-liver-disease
#3
Miaoting Yang, Zhijuan Chen, Chunxin Xiao, Waijiao Tang, Beijie Zhou
OBJECTIVE: To investigate the effects of sera from rats fed with Huganqingzhi tablets (HGT) on endoplasmic reticulum (ER) stress in a steatotic hepatocyte model of free fatty acids (FFAs)-induced nonalcoholic fatty liver disease (NAFLD) and explore the possible mechanism. METHODS: FFAs prepared by mixing oleic acid and palmitic acid at the ratio of 2:1. HepG2 cells were treated with the sera from rats fed with low-, moderate-or high-dose HGT (HGT sera) or sera of rats fed with fenofibrate (fenofibrate sera), followed by treatment with 1 mmol/L FFAs for 24 h to induce hepatic steatosis...
November 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/30510272/higher-lipid-turnover-and-oxidation-in-cultured-human-myotubes-from-athletic-versus-sedentary-young-male-subjects
#4
Jenny Lund, Siw A Helle, Yuchuan Li, Nils G Løvsletten, Hans K Stadheim, Jørgen Jensen, Eili T Kase, G Hege Thoresen, Arild C Rustan
In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO2max ) ≤ 46 ml/kg/min) and six healthy athletic (VO2max  > 60 ml/kg/min) young men were included. Myoblasts were cultured and differentiated to myotubes from satellite cells isolated from biopsy of musculus vastus lateralis. FA metabolism was studied in myotubes using [14 C]oleic acid. Lipid distribution was assessed by thin layer chromatography, and FA accumulation, lipolysis and re-esterification were measured by scintillation proximity assay...
December 3, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30506254/screening-the-lactic-acid-bacteria-converting-hydroxy-fatty-acid-from-unsaturated-fatty-acid
#5
Makoto Kanauchi
Hydroxyl fatty acids (HFAs) are used in widely diverse industrial applications, healthy functional foods, artificial food flavorings, and alcoholic beverages. A lactic acid bacterium (LAB), Lactobacillus sakei, hydroxylates oleic acid. Furthermore, the hydroxyl fatty acid was identified by GC-MS as 10-hydroxystearic acid. The Lactobacillus sakei hydroxylated more than 90% of the oleic acid in the medium at 15 °C after 30-48 h. The hydroxyl enzyme needs a coenzyme for an electron donor as NADPH. The enzyme is useful for assay with monitoring NADPH concentration used an A340 device...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30503959/new-approach-to-address-antibiotic-resistance-miss-loading-of-functional-membrane-microdomains-fmm-of-methicillin-resistant-staphylococcus-aureus-mrsa
#6
H S Nagendra Prasad, C S Karthik, H M Manukumar, L Mallesha, P Mallu
The synthesized potent piperazine analog ChDiPiCa was characterised by various spectroscopic techniques and for the first time evaluated functional membrane microdomain (FMM) disassembly in methicillin-resistant Staphylococcus aureus (MRSA). The ChDiPiCa showed excellent in vitro biocidal activity against MRSA at 26 μg/mL compared to the antibiotic streptomycin and bacitracin 14 μg/mL and 13 μg/mL at 10 μg concentration respectively. The membrane damaging property was confirmed by the SEM analysis...
November 29, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/30497975/corrigendum-to-the-ccch-type-transcription-factor-bnzfp1-is-a-positive-regulator-to-control-oleic-acid-levels-through-the-expression-of-diacylglycerol-o-acyltransferase-1-gene-in-brassica-napus-plant-physiol-biochem-132-november-2018-633-640
#7
Haiqiang Zhang, Zhenqian Zhang, Teng Xiong, Xinghua Xiong, Xianmeng Wu, Chunyun Guan, Gang Xiao
No abstract text is available yet for this article.
November 26, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/30496826/pufa-plasmalogens-attenuate-the-lps-induced-nitric-oxide-production-by-inhibiting-the-nf-kb-p38-mapk-and-jnk-pathways-in-microglial-cells
#8
Mohammed Youssef, Ahmed Ibrahim, Koichi Akashi, Md Shamim Hossain
The special lipids Plasmalogens (Pls) were reported to be reduced in the neurodegenerative brains such as Alzheimer's disease where a marked increase of glial activation is often observed. We previously found that a reduction of brain Pls can enhance the glial activation in murine brains. However, the detail role of Pls in the prevention of glial activation was mostly elusive. Here we report that the Pls, extracted from scallop (sPls), significantly inhibited the inducible form of nitric oxide synthase (NOS2) and the production of NO in LPS (lipopolysaccharide)-activated microglial cells...
November 26, 2018: Neuroscience
https://www.readbyqxmd.com/read/30496314/increased-triacylglycerol-fatty-acid-substrate-cycling-in-human-skeletal-muscle-cells-exposed-to-eicosapentaenoic-acid
#9
Nils G Løvsletten, Siril S Bakke, Eili T Kase, D Margriet Ouwens, G Hege Thoresen, Arild C Rustan
It has previously been shown that pretreatment of differentiated human skeletal muscle cells (myotubes) with eicosapentaenoic acid (EPA) promoted increased uptake of fatty acids and increased triacylglycerol accumulation, compared to pretreatment with oleic acid (OA) and palmitic acid (PA). The aim of the present study was to examine whether EPA could affect substrate cycling in human skeletal muscle cells by altering lipolysis rate of intracellular TAG and re-esterification of fatty acids. Fatty acid metabolism was studied in human myotubes using a mixture of fatty acids, consisting of radiolabelled oleic acid as tracer (14C-OA) together with EPA or PA...
2018: PloS One
https://www.readbyqxmd.com/read/30488102/lipid-acquisition-and-tissue-storage-in-hagfish-new-insights-from-an-ancient-vertebrate
#10
Alyssa M Weinrauch, Chris N Glover, Greg G Goss
Hagfishes are ancient vertebrates, which have the ability to tolerate nearly a year of food deprivation with energy during fasting maintained using lipid stores. While lipid transporters are evolutionarily conserved, there are relatively few studies examining mechanisms of lipid acquisition in teleosts and no reports in the primitive and evolutionarily important agnathans. We examined tissue lipid droplet distribution and used gut sac preparations to characterize uptake mechanisms of the monounsaturated fatty acid, oleic acid (OA; 18:1 cis-9), in the Pacific hagfish...
November 28, 2018: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/30487558/extra-virgin-olive-oil-more-than-a-healthy-fat
#11
REVIEW
Elena M Yubero-Serrano, Javier Lopez-Moreno, Francisco Gomez-Delgado, Jose Lopez-Miranda
The beneficial effects of a Mediterranean diet on human health and, in particular, on lowering risk of cardiovascular disease, has been mainly attributed to its high content to extra virgin olive oil (EVOO). While its main fatty acid, oleic acid, is considered important to these effects, EVOO has other biological properties that depend on, or are potentiated by other minor components of this oil. Initially, the mechanisms considered as possible causes of this cardioprotective effect of EVOO were based on the incidence on the so-called traditional risk factors (especially lipids and blood pressure)...
November 28, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/30486527/-research-on-influence-of-environment-factors-to-yield-and-quality-traits-of-perilla-frutescen
#12
Qi Shen, Ji-Xian Zhao, Xue-Bo Qiu, Zhi-Wei Shang, Xian-Ping Wang, Sen Yang, Jing Xu, Shi-Lin Chen
The research is aimed to study of the influence of environmental factors on the yield and quality traits, and find out the regularity of the growth and development of perilla. The main environmental factor data in six ecological area in Guizhou province were collected, and the correlation analysis with yield and quality traits of 15 perilla strains was conducted. The results showed that the cultivation environment has significant effects on the yield and quality traits of perilla. The effect of environment on main yield composed traits, contained grain number in top spike, effective panicle number per plant, plant height, top spike length, growth period, and thousand seed weight was degressive...
October 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/30484113/in-schizophrenia-deficits-in-natural-igm-isotype-antibodies-including-those-directed-to-malondialdehyde-and-azelaic-acid-strongly-predict-negative-symptoms-neurocognitive-impairments-and-the-deficit-syndrome
#13
Michael Maes, Buranee Kanchanatawan, Sunee Sirivichayakul, André F Carvalho
Schizophrenia is characterized by an interrelated activation of the immune-inflammatory response system (IRS) and the compensatory immune-regulatory system (CIRS), which downregulates the IRS. Deficit schizophrenia is characterized by a deficit in IgM-mediated autoimmune responses to tryptophan catabolites. The presence and correlates of IgM isotype antibodies to oxidative-specific epitopes (OSEs), nitroso (NO), and nitro (NO2 ) adducts in schizophrenia remain unknown. This study measured IgM antibodies to malondialdehyde (MDA), azelaic acid, phosphatidylinositol, oleic acid, NO-tryptophan, NO-albumin, NO-cysteinyl, and NO2 -tyrosine in a sample of 80 schizophrenia patients, divided into those with and those without deficit schizophrenia, and 38 healthy controls...
November 27, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/30483010/oxidative-stability-affective-and-discriminative-sensory-test-of-high-oleic-and-regular-peanut-oil-with-addition-of-oregano-essential-oil
#14
Rubén Olmedo, Pablo Ribotta, Nelson R Grosso
Peanut oil is widely used in food but is susceptible to oxidation. This study investigated the antioxidant stability of high oleic (HO: 78.85 g/100 g oleic acid) and regular (C: 43.85 g/100 g oleic acid) peanut oils with oregano essential oil (OEO) added as a natural antioxidant. OEO contained γ-terpinene (25.71%), carvacrol (16.73%) and terpinen-4-ol (16.17%) as the principal compounds. Thermal processing (60 °C for 28 days) of OEO increased the carvacrol and o -cymene contents and decreased the terpinen-4-ol, linalool and γ-terpinene levels...
December 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/30482968/effect-of-clove-extract-on-lipid-oxidation-antioxidant-activity-volatile-compounds-and-fatty-acid-composition-of-salted-duck-eggs
#15
Putri Widyanti Harlina, Meihu Ma, Raheel Shahzad, Mostafa Mahmoud Gouda, Ning Qiu
Antioxidant activity, lipid oxidation, fatty acid composition and volatile compounds of duck eggs supplemented with clove extract were monitored over the salting period. The results indicated that application of clove extracts significantly reduced 2-thiobarbituric acid reactive substances and anisidine values as well as the conjugated dienes levels during curing. Clove extracts along with salting time had significant effects on the fatty acid composition. The predominant fatty acid in salted duck eggs supplemented with clove extracts for 14 day of the salting process was oleic acid, followed by palmitic acid and arachidonic acid...
December 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/30480357/a-tunneling-dielectric-layer-free-floating-gate-nonvolatile-memory-employing-type-i-core-shell-quantum-dots-as-discrete-charge-trapping-tunneling-centers
#16
Chengyuan Yan, Jiamin Wen, Peng Lin, Zhenhua Sun
A nonvolatile memory with a floating gate structure is fabricated using ZnSe@ZnS core-shell quantum dots as discrete charge-trapping/tunneling centers. Systematical investigation reveals that the spontaneous recovery of the trapped charges in the ZnSe core can be effectively avoided by the type-I energy band structure of the quantum dots. The surface oleic acid ligand surrounding the quantum dots can also play a role of energy barrier to prevent unintentional charge recovery. The device based on the quantum dots demonstrates a large memory window, stable retention, and good endurance...
November 27, 2018: Small
https://www.readbyqxmd.com/read/30479514/effects-of-intensive-alfalfa-feeding-on-meat-quality-and-fatty-acid-profile-of-korean-native-black-goats
#17
Young-Hwa Hwang, Allah Bakhsh, Ishamri Ismail, Jung-Gyu Lee, Seon-Tea Joo
The aim of this study was to determine meat quality characteristics and fatty acid composition of Korean native black goats (KNBG) finished on intensive feeding of alfalfa (ALF) and conventional feeding of commercial concentrate pellets (CCP) with low-energy common grasses. Ten KNBG (12 months old) were divided into two groups and subjected to either ALF or CCP treatments. The goats were slaughtered after 6 months of feeding with experimental diets to investigate meat quality characteristics and fatty acid compositions of longissimus lumborum muscle...
October 2018: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/30479041/protective-effect-of-silkworm-pupa-oil-on-hydrochloric-acid-ethanol-induced-gastric-ulcer
#18
Xingyao Long, Xin Zhao, Wei Wang, Yu Zhang, Hongwei Wang, Xinqi Liu, Huayi Suo
BACKGROUND: Silkworm pupa is a traditional Chinese food that is rich in various saturated and unsaturated fatty acids. Unsaturated fatty acids have a certain protective effect on oxidative damage. The present study used an animal model to determine the protective effect of silkworm pupa oil on hydrochloric acid/ethanol-induced gastric ulcer. RESULTS: Silkworm pupa oil is rich in unsaturated fatty acids, including palmitoleic acid 63.4 g·kg-1 , oleic acid 249.1 g·kg-1 , linoleic acid 47...
November 26, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/30476796/rapid-and-direct-determination-of-fatty-acids-and-glycerides-profiles-in-schisandra-chinensis-oil-by-using-uplc-q-tof-ms-e
#19
Yuan Gao, Shimin Wu, Lei Feng
Fatty acids and glycerides are globally accepted quality and nutrition indicators of oils. Schisandra chinensis (S. chinensis) is a good functional oil source, with an oil content of 10-50% (dry weight). In this study, the UPLC-Q/TOF-MSE technique was developed to profile FFA and glycerides in the S. chinensis oils directly. The results showed that all of the 36 FFA calibration equations of the mixture standard had good linear relationships (R2  > 0.99). The limit of detection for the tested compounds ranged from 0...
November 19, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/30473730/bioconversion-of-cheese-whey-permeate-into-fungal-oil-by-mucor-circinelloides
#20
Lauryn G Chan, Joshua L Cohen, Gulustan Ozturk, Marie Hennebelle, Ameer Y Taha, Juliana Maria L N de Moura Bell
Background: Oleaginous fungi are efficient tools to convert agricultural waste streams into valuable components. The filamentous fungus Mucor circinelloides was cultivated in whey permeate, a byproduct from cheese production, to produce an oil-rich fungal biomass. Response surface methodology was used to optimize the fermentation conditions such as pH and temperature for increased biomass yield and lipid accumulation. Quantification and characterization of the fungal biomass oil was conducted...
2018: Journal of Biological Engineering
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