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Ruixing Liu, Xiaoming Yan
The aim of the present study was to examine whether activation of the nuclear factor E2‑related factor 2 (Nrf‑2)/heme oxygenase‑1 (HO‑1) antioxidant pathway in the cornea was involved in the protective effect of sulforaphane (SF) following keratoconus (KC) injury. Following epithelial debridement, collagenase type II was applied in KC groups at room temperature for 30 min. Following this, rabbits were administered with a subconjunctival (s.c.) injection of SF or placebo (maize oil) daily for a total of 2 weeks...
August 10, 2018: International Journal of Molecular Medicine
Breetha R, Ramaprasad Tr
This study addresses the effects of n-3 and n-6 fatty acids in maternal dyslipidemia induced inflammation over three generation in rats. The detailed protocol for animal feeding and mating is described in the methodology. Placenta and fetal liver were isolated on the eighteenth day of gestation and delivered pups after lactation were kept on their maternal diets. Compared to control and experimental groups, high-fat fed rats (HFL) had a higher level of cytokines and eicosanoids in serum (p < 0.05). Liver and uterine expression of cPLA-2, Cox-2, 5-Lox, EP-1, BLT-1, and ICAM-1 were higher (p < 0...
August 2018: Prostaglandins, Leukotrienes, and Essential Fatty Acids
Yuan Cao, Xiaoyan Zhou, Zixiao Yin, Xinbo Yu, Qing Yang, Qikun Guo, Duxue Tian, Xiaoliang Xiong, Guogang Xu, Xiaodong Kuang
The aim of this study is to investigate whether the lipoxin receptor agonist BML-111 exerts a protective effect against inflammation in a mouse model of chronic obstructive pulmonary disease (COPD) by regulating NLRP3 inflammasome activation and reactive oxygen species (ROS) production. In this study, mice were randomly divided into the following five groups: control group (Control), COPD model group (Model), BML-111 low-dose group (Low-BML), BML-111 high-dose group (High-BML) and Dexamethasone group (Dex)...
August 10, 2018: Prostaglandins & Other Lipid Mediators
Cheng Chaohui, Huang Wei, Wen Hongfeng, Zhang Yueliang, Peng Xiaoqin, Zhou Pingli, Ai Zhibing
Atherosclerosis is a complex chronic inflammatory disease that is characterized by the formation of lipid-rich plaques on the inner walls of the arteries. Inactive rhomboid protein 2 (iRhom2) was recently determined as a necessary regulator for the shedding of tumor necrosis factor-alpha (TNF-α) in immune cells. In the present study, we aimed to explore the effects of iRhom2 on the inflammatory response and oxidative stress induced by low-density lipoprotein (ox-LDL) in RAW264.7 and THP-1-derived macrophages...
August 7, 2018: Biochemical and Biophysical Research Communications
Yin Heng, Wu Mao, Ma Yong, Guo Yang, Qiuju Su, Jianwei Wang
This study was to investigate the effects of paeoniflorin (PA) on lipopolysaccharides (LPS)-induced nucleus pulposus cells. Our results demonstrated that PA significantly reduced lipopolysaccharides (LPS) induced inflammatory factors including interleukin-1β (IL-1β), tumor necrosis Factor-α (TNF-α) and interleukin-6 (IL-6) in nucleus pulposus cells. PA also decreased of malondialdehyde (MDA) and increased superoxide dismutase (SOD) induced by LPS. Moreover, the expression of the protein levels of Nrf-2/HO-1/HMGB1/NF-κB were increased after LPS challenge, which were significantly restored after PA...
August 7, 2018: Microbial Pathogenesis
Suhong Wang, Zhecheng Zhang, Shuxin Zhao
β-Amyloid (Aβ) species probably exert neurotoxic effects in Alzheimer's disease. In the present study, the effect of antioxidant plumbagin was tested against Aβ25-35-induced neurotoxicity in the SH-SY5Y cell line. Cell viability was determined using an MTT assay. Antioxidant status was analyzed through antioxidant enzyme activities, nuclear factor erythroid 2-related factor 2 (Nrf-2), and its downstream protein expressions. Inflammatory response was determined through nuclear factor-κB (NF-κB) pathway and cytokine expressions...
August 8, 2018: Neuroreport
Chen Li, Ruili Wang, Chunting Hu, Hui Wang, Qiaoya Ma, Songsheng Chen, Ya He
Pyridoxine is a water- soluble pyridine derivative. The effect of pyridoxine in cell models of Alzheimer's disease (AD), and the potential mechanisms involved, are not fully understood. In this study, the anti-AD effects of pyridoxine were studied in an AD cell model using a combination of techniques viz MTT assay, western blotting and assays for reactive oxygen species (ROS). Assays were also carried out to determine the mechanism underlying the antioxidant effects of pyridoxine. The results obtained revealed that pyridoxine exerted a protective potential against AD, attenuated ROS levels, decreased the expressions of cytoplasmic Nrf2, and upregulated whole-cell HO-1 expression...
July 30, 2018: Cellular and Molecular Biology
Meejung Ahn, Jeongtae Kim, Seunghyun Hong, Jungeun Kim, Hana Ko, Nam-Ho Lee, Gi-Ok Kim, Taekyun Shin
Nonalcoholic fatty liver disease is a serious liver disorder associated with oxidative stress. Black radish (Raphanus sativus L. var. niger) extract (BRE) can lower the risk of this disease. The hepatoprotective effect of BRE containing 3-(E)-(methylthio)methylene-2-pyrrolidinethione was evaluated in human hepatocyte carcinoma (HepG2) cells and in rat livers with carbon tetrachloride (CCl4 )-induced hepatic injury. BRE was administered at 125, 250, 500, and 1000 μg/mL to the oleic acid-induced HepG2 cells...
August 1, 2018: Journal of Medicinal Food
Xinrui Zhang, Mengya Wang, Shanshan Teng, Di Wang, Xin Li, Xiaofeng Wang, Weiwei Liao, Di Wang
A facile method of assembling oxindole and phthalide units through a Lewis based catalyzed allylic alkylation reaction of Morita-Baylis-Hillman carbonates of isatins and 3-cyanophthalides was recently developed. The method efficiently delivers a hybrid of 3,3'-disubstituted oxindole with a valuable phthalide pharmacophore. In the present study, we proved the deleterious effects of 5h2c, a screened synthesis compound, against hepatocellular carcinoma (HCC) in both in vitro and in vivo models. 5h2c strongly decreased cell viability, caused over-release of lactate dehydrogenase, inhibited cell migration, and enhanced the apoptosis rate in HepG2 and PLC/PRF/5 cells...
July 25, 2018: Chemico-biological Interactions
Ling Zhang, Xin-Rong Fan, Hui Xie, Qing-Hu He, Yu-Song Nie, Min Zhang, Miao Yan
Benign prostatic hyperplasia (BPH) is a common disease in the current ageing male population. This research aims to study the effects of Kelong-Capsules (KLC) on testosterone-induced BPH. Thirty rats were randomly divided into normal group, model group, and three treatment groups. Three treatment groups were given KLC (3.6 g/kg), KLC (7.2 g/kg), and finasteride (0.9 mg/kg), respectively, for 28 days after establishing the animal model. The BPH rat models were evaluated by Traditional Chinese Medicine (TCM) symptoms and prostate index (PI)...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Xu-Hua Ge, Li Shao, Guo-Ji Zhu
To investigate the potential neuroprotection of oxymatrine in hypoxic-ischemic injury in rat's brain and the associated underlying mechanisms, modified neurological severity scores (mNSS) for neurological functional deficits, 2,3,5-triphenyl-tetrazolium chloride (TTC) staining for infarct volume, TUNEL assay and flow cytometry analysis for apoptosis were assessed. The expressions of Akt, glycogen synthase kinase 3 beta (GSK3β), phosphorylated Akt (p-Akt), phosphorylated GSK3β (p-GSK3β), nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1) were measured by western blot...
July 21, 2018: Metabolic Brain Disease
Fares E M Ali, Amany A Azouz, Adel G Bakr, Amira M Abo-Youssef, Ramadan A M Hemeida
The present study was designed to investigate the potential protective effects of diosmin (DS) and/or sildenafil against bile duct ligation (BDL). In order to achieve this goal, BDL was performed to induce liver cirrhosis, DS (100 mg/kg/day, p.o.) and sildenafil (10 mg/kg, twice daily, p.o.) were administrated alone or in combination 24 h after the surgical operation and lasted for 4 weeks. Liver function biomarkers, fibrotic markers, oxidative stress markers, mRNA expression of NF-κB-p65, P38 -MAPK, Nrf-2, and Keap-1, as well as protein expression of cytoglobin, NF-κB-p65, Nrf-2, iNOS and eNOS were investigated concomitantly with histopathological study...
July 17, 2018: Food and Chemical Toxicology
C Liu, L Zhong, X-L Tian, Y-C Han
OBJECTIVE: To explore the effect of 8-MOP on the blood-brain barrier in mice model of cerebral infarction and the underlying mechanism. MATERIALS AND METHODS: Middle cerebral artery occlusion (MCAO) model was established to induce permanent cerebral infarction. The neurological function was observed and scored by the modified longa score method after model establishment. Besides, the water content of brain tissue was measured by the standard dry weight method. Evans blue exudation rate was used to evaluate the effect of 8-MOP on the permeability of the blood-brain barrier...
July 2018: European Review for Medical and Pharmacological Sciences
Fabiana P Costa-Fraga, Gleisy K Goncalves, Fernando P Souza-Neto, Adelina M Reis, Luciano As Capettini, Robson As Santos, Rodrigo A Fraga-Silva, Nikolaos Stergiopulos, Rafaela F da Silva
The vasodilatory effect of angiotensin-(1-7) seems to vary between sexes, and estradiol (E2) can modulate the magnitude of the Ang-(1-7) vasodilatory response in female rats. However, there are few studies addressing the influence of sex on the age-related vasodilatory effect of Ang-(1-7). Here, we evaluated the vasodilatory response to Ang-(1-7) on vascular ageing. Ang-(1-7) dose-response curves were determined in mice aortic rings from males (old and young) and females (E2 treated/non-treated old and young) mounted in an isolated organ chamber...
July 2018: Journal of the Renin-angiotensin-aldosterone System: JRAAS
Saima Ali, Muhammad Rashid Khan, Sayed Afzal Shah, Riffat Batool, Sonia Maryam, Muhammad Majid, Zartash Zahra
In the present study the antioxidant capacity of Periploca hydaspidis was assessed through various in vitro assays and by the hepatoprotective potential on CCl4 induced toxicity in rat. Phytochemical analysis of different extracts of P. hydaspidis indicated existence of various phytochemical classes. HPLC-DAD analysis of methanol extract indicated the existence of rutin, gallic acid and caffeic acid. Total phenolic (TPC) and total flavonoid content (TFC) exhibited significant (p < 0.05) correlation with 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide, hydroxyl ion, inhibition of β-carotene oxidation, iron chelation, reducing power and total antioxidant capacity...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jianjun Xu, Cai Lin, Tingting Wang, Peng Zhang, Zhengjun Liu, Caijiao Lu
BACKGROUND/AIMS: Ergosterol (ER) is the primary sterol found in fungi and is named after the ergot fungus. A variety of pharmacological activities have been reported for ER, including antioxidative, anti-proliferative, and anti-inflammatory effects, although its role in sepsis remains unclear. METHODS: The protective effect of ER on lipopolysaccharide (LPS)-induced sepsis myocardial injury was evaluated both in vivo and in vitro. Rats were pretreated with ER and then with LPS...
July 18, 2018: Cellular Physiology and Biochemistry
Jun Yang, Jiagui Huang, Changbo Shen, Wei Cheng, Pingping Yu, Li Wang, Fanren Tang, Shuang Guo, Qin Yang, John Zhang
Recent studies have indicated that resveratrol has protective effects against cerebral ischemia/reperfusion injury. However, the best therapeutic time for resveratrol treatment after acute ischemic stroke remains unknown. We aim to investigate whether resveratrol, administrated at different times after neuronal oxygen and glucose deprivation/reoxygenation (OGD/R) reduced neuronal injury in vitro. There were six experimental groups: normal, model, resveratrol pretreatment, resveratrol post-treatment, resveratrol OGD-treatment, and resveratrol whole-processing group...
January 1, 2018: Cell Transplantation
Viviana Greco, Alida Spalloni, Victor Corasolla Carregari, Luisa Pieroni, Silvia Persichilli, Nicola B Mercuri, Andrea Urbani, Patrizia Longone
Hydrogen sulfide (H₂S) is an endogenous gasotransmitter recognized as an essential body product with a dual, biphasic action. It can function as an antioxidant and a cytoprotective, but also as a poison with a high probability of causing brain damage when present at noxious levels. In a previous study, we measured toxic liquoral levels of H₂S in sporadic amyotrophic lateral sclerosis (ALS) patients and in the familial ALS (fALS) mouse model, SOD1G93A. In addition, we experimentally demonstrated that H₂S is extremely and selectively toxic to motor neurons, and that it is released by glial cells and increases Ca2+ concentration in motor neurons due to a lack of ATP...
July 10, 2018: Antioxidants (Basel, Switzerland)
Agnese Gugliandolo, Federica Pollastro, Gianpaolo Grassi, Placido Bramanti, Emanuela Mazzon
Inflammation and oxidative stress play main roles in neurodegeneration. Interestingly, different natural compounds may be able to exert neuroprotective actions against inflammation and oxidative stress, protecting from neuronal cell loss. Among these natural sources, Cannabis sativa represents a reservoir of compounds exerting beneficial properties, including cannabigerol (CBG), whose antioxidant properties have already been demonstrated in macrophages. Here, we aimed to evaluate the ability of CBG to protect NSC-34 motor neurons against the toxicity induced from the medium of LPS-stimulated RAW 264...
July 8, 2018: International Journal of Molecular Sciences
Wei Wang, Xiao Wang, Xian-Sheng Zhang, Chao-Zhao Liang
Oxidative stress and inflammatory responses are closely implicated in the progression of renal interstitial fibrosis, thereby leading to chronic kidney disease. Cryptotanshinone(CTS) is a natural compound involved in antioxidant and anti-inflammatory activities. We evaluated the effects of CTS on inflammation and oxidative stress in obstructed kidneys. Mice received gastric gavage of CTS from 7 days before unilateral ureteral obstruction operation to one week after surgery. Administration of CTS at 50 and 100 mg·kg-1 ·day-1 significantly decreased collagen production, as shown by Masson staining...
July 4, 2018: Basic & Clinical Pharmacology & Toxicology
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