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endothelial to mesenchymal

Muhammad Shafiq, Lina Wang, Dengke Zhi, Qiuying Zhang, Kai Wang, Lianyong Wang, Dong-Hwee Kim, Deling Kong, Soo Hyun Kim
In situ blood vessel regeneration through host stem/progenitor cell mobilization may hold great promise for vascular reconstruction. Neuropeptide substance P (SP) has been shown to accelerate tissue repair by endogenous cell mobilization and recruitment. This study was aimed to evaluate the vascular regeneration potential of SP and heparin co-tethered vascular grafts. Polycaprolactone (PCL), PCL/SP-conjugated poly(L-lactide-co-ε-caprolactone) (PLCL-SP) (SP), and PCL/PLCL-SP/heparin-conjugated PLCL (Hep/SP) vascular grafts were implanted as rat abdominal aorta substitutes for up to 2 weeks and 4 weeks...
October 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Radoslaw Litwinowicz, Bogusław Kapelak, Jerzy Sadowski, Anna Kędziora, Krzysztof Bartus
Ischemic heart disease is a major cause of death and disabilities worldwide. Unfortunately, not all patients are suitable for direct revascularization. Cell-based therapies may be alternative options because of their potential to promote neovascularisation and endothelial repair, improving myocardial perfusion. The success of cell-based therapies depends on the type of implanted stem cells, delivery method and underlying disease. Several different cell populations including bone marrow-derived mononuclear cells (MNCs), mesenchymal stromal cells (MSCs), CD34+, CD133+, endothelial progenitor cells, adipose-derived mesenchymal stromal cells (ASCs) and stem cells from placenta and umbilical cord have been investigated...
September 2018: Kardiochirurgia i Torakochirurgia Polska, Polish Journal of Cardio-Thoracic Surgery
Elvira De Giglio, Maria A Bonifacio, Ana M Ferreira, Stefania Cometa, Zhi Yuan Ti, Antonella Stanzione, Kenny Dalgarno, Piergiorgio Gentile
The development of in vitro 3D models to get insights into the mechanisms of bone regeneration could accelerate the translation of experimental findings to the clinic, reducing costs and duration of experiments. This work explores the design and manufacturing of multi-compartments structures in poly(ε-caprolactone) (PCL) 3D-printed by Fused Filament Fabrication technique. The construct was designed with interconnected stalls to host stem cells and endothelial cells. Cells were encapsulated within an optimised gellan gum (GG)-based hydrogel matrix, crosslinked using strontium (Sr2+ ) ions to exploit its bioactivity and finally, assembled within compartments with different sizes...
October 11, 2018: Scientific Reports
Angela Gutermuth, Jessika Maassen, Emely Harnisch, Daniel Kuhlen, Alexis Sauer-Budge, Claudia Skazik-Voogt, Katrin Engelmann
PURPOSE: Loss of corneal endothelial cells (CECs) bears disastrous consequences for the patient, including corneal clouding and blindness. Corneal transplantation is currently the only therapy for severe corneal disorders. However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells...
October 10, 2018: Cornea
Chun-Jie Gu, Feng Xie, Bing Zhang, Hui-Li Yang, Jiao Cheng, Yin-Yan He, Xiao-Yong Zhu, Da-Jin Li, Ming-Qing Li
BACKGROUND/AIMS: Uterus endometrial cancer (UEC) is the common malignancy among gynecologic cancers, and most of them are type I estrogen-dependent UEC. Diabetes is well-known risk factor for the development of UEC. However, the underlying link between high glucose (HG) and the estrogen receptor in UEC remains unclear. Epithelial-mesenchymal transition (EMT) has also been shown to occur during the initiation of metastasis in cancer progression. The aim of this study was to determine the relationships and roles of HG, estrogen receptor and EMT in the growth and migration of UEC...
October 11, 2018: Cellular Physiology and Biochemistry
Raziel Rojas-Rodriguez, Jorge Lujan-Hernandez, So Yun Min, Tiffany DeSouza, Patrick Teebagy, Anand Desai, Heather Tessier, Robert Slamin, Leah Siegel-Reamer, Cara Berg, Angel Baez, Janice Lalikos, Silvia Corvera
Adipose tissue is used extensively in reconstructive and regenerative therapies, but transplanted fat often undergoes cell death, leading to inflammation, calcification and requirement for further revision surgery. Previously we have found that mesenchymal progenitor cells within human adipose tissue can proliferate in 3-dimensional culture under pro-angiogenic conditions. These cells (Primed ADipose progenitor cells=PADS) robustly differentiate into adipocytes in-vitro (ad-PADS). The goal of the present study is to determine whether ad-PADS can form structured adipose tissue in-vivo, with potential for use in surgical applications...
October 11, 2018: Tissue Engineering. Part A
Andrew J Kim, Christina M Alfieri, Katherine E Yutzey
Epithelial-to-mesenchymal transition (EMT) enables stationary epithelial cells to exhibit migratory behavior and is the key step that initiates heart valve development. Recent studies suggest that EMT is reactivated in the pathogenesis of myxomatous valve disease (MVD), a condition that involves the progressive degeneration and thickening of valve leaflets. These studies have been limited to in vitro experimentation and reliance on histologic costaining of epithelial and mesenchymal markers as evidence of EMT in mouse and sheep models of valve disease...
October 10, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Aya Tal, Reshef Tal, Shafiq Shaikh, Stephanie Gidicsin, Ramanaiah Mamillapalli, Hugh S Taylor
Cell fusion is involved in the development of some adult organs, is implicated in the pathogenesis of specific types of cancer, and is known to participate in repair/regeneration processes mediated by bone-marrow derived cells (BMDCs). Endometriosis is a disease characterized by growth of functional endometrial tissue outside of the uterine cavity. Endometriosis shares some molecular properties with cancer and BMDCs home to endometriosis lesions in a mouse model. Our objective was to determine if cell fusion can occur in endometriosis and establish whether bone marrow derived cells participate in cell fusion events in lesions...
October 9, 2018: Biology of Reproduction
Ula V Jurkunas
The corneal endothelium (CE) is vital for maintaining the water balance and clarity of the cornea. The CE is a cell layer that is particularly susceptible to aging because of its postmitotic arrest, high metabolic activity involving pumping of ions, and lifelong exposure to ultraviolet light. Despite gradual age-related cell loss, a sufficient number of CE cells are preserved during the lifespan of an individual. However, in conditions such as Fuchs endothelial corneal dystrophy (FECD), permanent loss of CE cells leads to corneal edema and loss of vision requiring corneal transplantation...
November 2018: Cornea
Susumu Tajiri, Shuichiro Yamanaka, Toshinari Fujimoto, Kei Matsumoto, Atsuhiro Taguchi, Ryuichi Nishinakamura, Hirotaka James Okano, Takashi Yokoo
Kidney regeneration from pluripotent stem cells is receiving a lot of attention because limited treatments are currently available for chronic kidney disease (CKD). It has been shown that uremic state in CKD is toxic to somatic stem/progenitor cells, such as endothelial progenitor and mesenchymal stem cells, affecting their differentiation and angiogenic potential. Recent studies reported that specific abnormalities caused by the non-inherited disease are often retained in induced pluripotent stem cell (iPSC)-derived products obtained from patients...
October 8, 2018: Scientific Reports
Su-Yan Bian, Hong-Bin Liu, Hong-Wei Liu, Qi-Wei Zhu
OBJECTIVE: To explore the proangiogenic activity of exsomes released by human umbilical cord mesenchymal stem cells (MSCs) stimulated by erythropoietin and platelet-derived growth factor BB (PDGF-BB). METHODS: Human umbilical cord-derived MSCs were seeded and maintained in culture overnight. The media were then replaced by alpha-MEM containing EPO (1 U/ml) and/or PDGF-BB (50 ng/ml), and the culture was maintained for 72 hours. The exosomes from the culture supernatants were isolated with a routine ultra-catrifagation method...
October 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Abdullah Md Sheikh, Shozo Yano, Shingo Mitaki, Md Ahsanul Haque, Shuhei Yamaguchi, Atsushi Nagai
A human mesenchymal stem cell line (B10) transplantation has been shown to improve ischemia-induced neurological deficits in animal stroke models. To understand the underlying mechanism, we have investigated the effects of B10 transplantation on cerebral angiogenesis in a rat middle cerebral artery occlusion (MCAO) model. B10 cells were transplanted intravenously 24 h after MCAO. Immunofluorescence staining results showed that compared to PBS-groups, vWF positive vessel and endoglin positive new vessels were increased in B10-transplanted MCAO groups in the lesion areas...
October 3, 2018: Experimental Neurology
Patrick Ming-Kuen Tang, Ying-Ying Zhang, Hui-Yao Lan
Transforming growth factor-β (TGF-β) is a crucial mediator in tissue fibrosis that promotes accumulation of extracellular matrix (ECM), myofibroblasts to epithelial⁻mesenchymal transition (EMT), endothelial-mesenchymal transition (EndoMT), and apoptosis via canonical and noncanonical signaling pathways. In the past decades, a number of microRNAs have been reported to participate in TGF-β-mediated tissue scarring; however, the roles of long noncoding RNAs (lncRNAs) in fibrogenesis remain largely unknown...
October 4, 2018: Non-Coding RNA
Paola Di Benedetto, Vasiliki Liakouli, Piero Ruscitti, Onorina Berardicurti, Francesco Carubbi, Noemi Panzera, Salvatore Di Bartolomeo, Giuliana Guggino, Francesco Ciccia, Giovanni Triolo, Paola Cipriani, Roberto Giacomelli
BACKGROUND: Fibrosis may be considered the hallmark of systemic sclerosis (SSc), the end stage triggered by different pathological events. Transforming growth factor-β (TGF-β) and platelet-derived growth factor BB (PDGF-BB) are profibrotic molecules modulating myofibroblast differentiation and proliferation, respectively. There is evidence linking CD248 with these two molecules, both highly expressed in patients with SSc, and suggesting that CD248 may be a therapeutic target for several diseases...
October 3, 2018: Arthritis Research & Therapy
Kenichi Ogata, Mayu Matsumura, Masafumi Moriyama, Wataru Katagiri, Hideharu Hibi, Seiji Nakamura
Recently, several studies have demonstrated that intravenous administration of mesenchymal stem cells (MSCs) improve medication-related osteonecrosis of the jaw (MRONJ), and paracrine effects of secretomes from MSCs have been hypothesized as the primary contributors. These secretomes in conditioned media from human MSCs (MSC-CM) were previously demonstrated to promote bone and tissue regeneration. Because MSC-CM contain cytokines monocyte chemoattractant protein (MCP)-1, insulin growth factor (IGF)-1, and vascular endothelial growth factor (VEGF) at relatively higher concentrations than other factors, these cytokines were considered as relevant active factors for tissue regeneration...
March 2018: JBMR Plus
Duong Thi Bich Thuan, Hatem Zayed, Ali H Eid, Haissam Abou-Saleh, Gheyath K Nasrallah, Arduino A Mangoni, Gianfranco Pintus
Systemic sclerosis (SSc), an autoimmune disease that is associated with a number of genetic and environmental risk factors, is characterized by progressive fibrosis and microvasculature damage in the skin, lungs, heart, digestive system, kidneys, muscles, joints, and nervous system. These abnormalities are associated with altered secretion of growth factor and profibrotic cytokines, such as transforming growth factor-beta (TGF-β), interleukin-4 (IL-4), platelet-derived growth factor (PDGF), and connective-tissue growth factor (CTGF)...
2018: Frontiers in Immunology
Taimoor H Qazi, Julia C Berkmann, Janosch Schoon, Sven Geißler, Georg N Duda, Aldo R Boccaccini, Evi Lippens
Vascularization of the fracture site and cell-mediated deposition of the mineralized matrix are crucial determinants for successful bone regeneration after injury. Ceramic biomaterials such as bioactive glasses (BAGs) that release bioactive ions have shown promising results in bone defect regeneration. However, it remains unclear how the dosage and composition of bioactive ions influence the angiogenic and osteogenic behavior of primary human mesenchymal stromal cells (MSCs). Here, we show that exposure to ionic dissolution products from 1393 and 45S5 BAGs can evoke distinct angiogenic and osteogenic responses from primary MSCs in a dose- and composition-dependent manner...
October 3, 2018: Journal of Biomedical Materials Research. Part A
Hyun-Kyung Kim, Seul-Gi Lee, Seung-Woo Lee, Bae Jun Oh, Jae Hyeon Kim, Jeong A Kim, Guisera Lee, Jae-Deog Jang, Young Ae Joe
Mesenchymal stromal/stem cells (MSCs) have been developed as a promising source for cell-based therapies of ischemic disease. However, there are some hurdles in their clinical application such as poor cell engraftment and inconsistent stem cell potency. In this study, we sought to find biomarkers for predicting potency of MSCs for proangiogenic therapy to improve their beneficial effects. Large variations were observed in proangiogenic factor secretion profiles of conditioned media derived from nine different donor-derived Wharton's jelly (WJ)-derived MSCs and 8 factors among 55 angiogenesis-related factors were secreted at considerable levels...
October 3, 2018: Stem Cells
S Maleki, K A Cottrill, F-A Poujade, A Bhattachariya, O Bergman, J R Gådin, N Simon, K Lundströmer, A Franco-Cereceda, H M Björck, S Y Chan, P Eriksson
BACKGROUND: An individual with a bicuspid aortic valve (BAV) runs a substantially higher risk of developing aneurysm in the ascending aorta compared to the normal population with tricuspid aortic valves (TAV). Aneurysm formation in patients with BAV and TAV is known to be distinct at the molecular level but the underlying mechanisms are undefined. Here, we investigated the still incompletely described role of microRNAs (miRNAs), important post-transcriptional regulators of gene expression, in such aortic disease of patients with BAV as compared with TAV...
October 2, 2018: Journal of Internal Medicine
Hotaka Kawai, Hidetsugu Tsujigiwa, Chong Huat Siar, Keisuke Nakano, Kiyofumi Takabatake, Masae Fujii, Mei Hamada, Ryo Tamamura, Hitoshi Nagatsuka
Background: The tumor microenvironment and its stromal cells play an important role in cancer development and metastasis. Bone marrow-derived cells (BMDCs), a rich source of hematopoietic and mesenchymal stem cells, putatively contribute to this tumoral stroma. However their characteristics and roles within the tumor microenvironment are unclear. In the present study, BMDCs in the tumor microenvironment were traced using the green fluorescent protein (GFP) bone marrow transplantation model. Methods: C57BL/6 mice were irradiated and rescued by bone marrow transplantation from GFP-transgenic mice...
2018: International Journal of Medical Sciences
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