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endothelial to mesenchymal

Yongliang Jiang, Xuanfen Zhou, Ruicheng Hu, Aiguo Dai
Endothelial-to-mesenchymal transition (EndMT), which is characterized by increased proliferation, migration and invasion of endothelial cells, increased expression of mesenchymal markers and reduced expression of endothelial markers, has been reported to be closely related to the pathogenesis of several diseases, including pulmonary fibrosis. Resistin-like molecule-β (RELM-β), also known as "found in inflammatory zone 2" (FIIZ2), plays an essential role in airway remodeling and pulmonary fibrosis; however, its role and mechanism in EndMT remain unclear...
August 15, 2018: International Journal of Biochemistry & Cell Biology
Julia Frank, Maximilian Richter, Chiara de Rossi, Claus-Michael Lehr, Kathrin Fuhrmann, Gregor Fuhrmann
Extracellular vesicles (EVs) are natural nanoparticles that play important roles in intercellular communication and are increasingly studied for biosignalling, pathogenesis and therapy. Nevertheless, little is known about optimal conditions for their transfer and storage, and the potential impact on preserving EV-loaded cargoes. We present the first comprehensive stability assessment of different widely available types of EVs during various storage conditions including -80 °C, 4 °C, room temperature, and freeze-drying (lyophilisation)...
August 17, 2018: Scientific Reports
Yun-Zhong Nie, Yun-Wen Zheng, Kei Miyakawa, Soichiro Murata, Ran-Ran Zhang, Keisuke Sekine, Yasuharu Ueno, Takanori Takebe, Takaji Wakita, Akihide Ryo, Hideki Taniguchi
Therapies against hepatitis B virus (HBV) have improved in recent decades; however, the development of individualized treatments has been limited by the lack of individualized infection models. In this study, we used human induced pluripotent stem cell (hiPSC) to generate a functional liver organoid (LO) that inherited the genetic background of the donor, and evaluated its application in modeling HBV infection and exploring virus-host interactions. To establish a functional hiPSC-LO, we cultured hiPSC-derived endodermal, mesenchymal, and endothelial cells with a chemically defined medium in a three-dimensional microwell culture system...
August 14, 2018: EBioMedicine
Qiang Su, Yuhan Sun, Ziliang Ye, Huafeng Yang, Lang Li
The endothelial-to-mesenchymal transition (EndMT) of endothelial cells contributes to the development of atherosclerosis. Oxidized low density lipoprotein (ox-LDL) is a highly risk factor for atherosclerosis. However, whether ox-LDL causes EndMT and the underlying mechanism are unclear. We report here that ox-LDL treatment is able to induce EndMT in human aortic endothelial cells (HAECs), and that the ox-LDL-induced EndMT is strictly dependent on the presence of its innate receptor, ox-LDL Receptor-1 (LOX-1)...
July 30, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Wenbo Zhang, Chao Huang, Aijun Sun, Liang Qiao, Xi Zhang, Junlong Huang, Xuejun Sun, Xiangqun Yang, Shanquan Sun
Senescence has become a hot point issue in recent decades and requires urgent attention. As a novel and effective antioxidant, hydrogen has been proved to alleviate cellular senescence in endothelial cells in vitro. However, the effects and mechanisms of hydrogen on senescence in vivo are still unclear. In the present study, 12-month-old Sprague Dawley (SD) rats were intraperitoneal administration of hydrogen-rich saline (HRS, 10 ml/kg). Subsequently, bone marrow-derived stem cells (BMSCs) were harvested for the detection of hydrogen antisenescence effects and mechanisms...
July 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
J Parrish, K S Lim, K Baer, G J Hooper, T B F Woodfield
Traditional 2D monolayer cell cultures and submillimeter 3D tissue construct cultures used widely in tissue engineering are limited in their ability to extrapolate experimental data to predict in vivo responses due to their simplistic organization and lack of stimuli. The rise of biofabrication and bioreactor technologies has sought to address this through the development of techniques to spatially organize components of a tissue construct, and devices to supply these tissue constructs with an increasingly in vivo-like environment...
August 17, 2018: Lab on a Chip
Mazen M Jamil Al-Obaidi, Mohd Nasir Mohd Desa
This review aims to elucidate the different mechanisms of blood brain barrier (BBB) disruption that may occur due to invasion by different types of bacteria, as well as to show the bacteria-host interactions that assist the bacterial pathogen in invading the brain. For example, platelet-activating factor receptor (PAFR) is responsible for brain invasion during the adhesion of pneumococci to brain endothelial cells, which might lead to brain invasion. Additionally, the major adhesin of the pneumococcal pilus-1, RrgA is able to bind the BBB endothelial receptors: polymeric immunoglobulin receptor (pIgR) and platelet endothelial cell adhesion molecule (PECAM-1), thus leading to invasion of the brain...
August 16, 2018: Cellular and Molecular Neurobiology
Francisco O'Valle, Juan G de Buitrago, Pedro Hernández-Cortés, Miguel Padial-Molina, Vicente Crespo-Lora, Marien Cobo, David Aguilar, Pablo Galindo-Moreno
This study aimed to analyze the expression of Musashi-1 (MSI1) in maxillary native bone and grafted bone after maxillary sinus floor elevation. To do so, fifty-seven bone biopsies from 45 participants were studied. Eighteen samples were collected from native bone while 39 were obtained 6 months after maxillary sinus grafting procedures. Musashi-1 was analyzed by immunohistochemistry and RT-PCR. MSI1 was detected in osteoblasts and osteocytes in 97.4% (38/39) of grafted areas. In native bone, MSI1 was detected in only 66...
August 16, 2018: Scientific Reports
Zheng Chen, Qianqian Chen, Haitao Du, Lijuan Xu, Jun Wan
The present study intended to observe the homing capability and therapeutic effect of CXC chemokine receptor 4 (CXCR-4) gene overexpressed bone marrow mesenchymal stem cells (BMSCs) on colitis, and to study the possible mechanisms involved. BMSCs were derived from male BALB/c mice and CXCR-4 gene was transfected into BMSCs by the utilization of the lentiviral vector. The expression of CXCR-4 gene was analyzed and the biological characteristics, and vitality of BMSCs and CXCR-4 gene overexpressed BMSCs (CXCR-BMSCs) were detected...
August 2018: Experimental and Therapeutic Medicine
Tatiana Lopatina, Cristina Grange, Valentina Fonsato, Marta Tapparo, Alessia Brossa, Sofia Fallo, Adriana Pitino, Maria Beatriz Herrera-Sanchez, Sharad Kholia, Giovanni Camussi, Benedetta Bussolati
Human liver stem-like cells (HLSC) and derived extracellular vesicles (EVs) were previously shown to exhibit anti-tumor activity. In the present study, we investigated whether HLSC-derived EVs (HLSC-EVs) were able to inhibit tumor angiogenesis in vitro and in vivo, in comparison with EVs derived from mesenchymal stem cells (MSC-EVs). The results obtained indicated that HLSC-EVs, but not MSC-EVs, inhibited the angiogenic properties of tumor-derived endothelial cells (TEC) both in vitro and in vivo in a model of subcutaneous implantation in Matrigel...
August 15, 2018: International Journal of Cancer. Journal International du Cancer
Maciej Liczek, Iga Panek, Piotr Damiański, Olga Jęczeń, Jędrzej Jaźwiec, Piotr Kuna, Michał Panek
The aim of the study was to find out scientific evidence on the possible use of the combined angiotensin II receptor antagonist and neprilysin inhibitors (ARNI) in patients with right heart failure (RHF) in the course of chronic obstructive pulmonary disease (COPD). It has been proven that a lack of neprilysin or its reduced expression in hypoxia leads to exacerbation of pulmonary arterial remodelling (PAR) or pulmonary hypertension (PH) in the mechanism related to the platelet-derived growth factor (PDGF) resulting in the proliferation and migration of pulmonary artery smooth muscle cells and endothelial-to-mesenchymal transition...
August 15, 2018: Advances in Respiratory Medicine
Kyohei Kin, Takao Yasuhara, Masahiro Kameda, Yousuke Tomita, Michiari Umakoshi, Ken Kuwahara, Ittetsu Kin, Naoya Kidani, Jun Morimoto, Mihoko Okazaki, Tatsuya Sasaki, Naoki Tajiri, Cesario V Borlongan, Isao Date
Despite the advances in pharmacological therapies, only the half of depressed patients respond to currently available treatment. Thus, the need for further investigation and development of effective therapies, especially those designed for treatment-resistant depression, has been sorely needed. Although antidepressant effects of mesenchymal stem cells (MSCs) have been reported, the potential benefit of this cell therapy on treatment-resistant depression is unknown. Cell encapsulation may enhance the survival rate of grafted cells, but the therapeutic effects and mechanisms mediating encapsulation of MSCs remain unexplored...
August 14, 2018: Molecular Psychiatry
Cinzia Rota, Marina Morigi, Domenico Cerullo, Martino Introna, Ornella Colpani, Daniela Corna, Chiara Capelli, Ton J Rabelink, Danielle G Leuning, Daniela Rottoli, Ariela Benigni, Carlamaria Zoja, Giuseppe Remuzzi
BACKGROUND: Mesenchymal stromal cell (MSC)-based therapy is a promising strategy for preventing the progression of chronic kidney disease (CKD), with the potential to induce tissue regeneration. In search of the best cellular source we compared, in the rat model of adriamycin (ADR) nephropathy, the regenerative potential of human stromal cells of non-renal origin, such as bone marrow (bm) MSCs and umbilical cord (uc) MSCs, with that of newly discovered stromal cells of renal origin, the kidney perivascular cells (kPSCs) known to exhibit tissue-specific properties...
August 14, 2018: Stem Cell Research & Therapy
Yu-Kun Li, Yuan-Jie Xie, Dai-Chao Wu, Shuang-Lian Long, Shengsong Tang, Zhong-Cheng Mo
The novel neurite outgrowth inhibitor B (Nogo‑B) receptor (NgBR) is specific for Nogo‑B, which is highly expressed in various human organs and cells, including the lung, liver, kidney, smooth muscle cells, blood vessel endothelial cells and inflammatory cells. Previous studies have indicated that NgBR directly interacts with Nogo‑B and is able to independently influence lipid and cholesterol homeostasis, angiogenesis, N‑glycosylation, the epithelial-mesenchymal transition, the chemotaxis of endothelial cells and cellular proliferation and apoptosis...
August 9, 2018: International Journal of Oncology
Bettina Hafen, Susanne Wiesner, Katrin Schlegelmilch, Alexander Keller, Lothar Seefried, Regina Ebert, Heike Walles, Franz Jakob, Norbert Schütze
Multipotent adult stem cells/precursor cells, especially of the mesenchymal and endothelial lineage, may have great potential for bone tissue engineering. Although their potential is highly recognized, not much is known about the underlying molecular mechanisms that initiate the regeneration process, connect osteogenesis, and angiogenesis and, finally, orchestrate renewal of bone tissue. Our study addressed these questions by generating two in vitro cell culture models to examine the changes in the global gene expression patterns of endothelial precursor cells and mesenchymal stem cells after 24 hours of either humoral (conditioned medium) or direct cell-cell interaction (co-culture)...
August 13, 2018: Journal of Cellular Biochemistry
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
Chunli Zhang, Yifei Du, Hua Yuan, Fei Jiang, Ming Shen, Yuli Wang, Ruixia Wang
Osteoporosis and vascular lesions induced by glucolipotoxicity are common complications of diabetes mellitus (DM). In order to deal with these complications, we designed a new therapeutic strategy, i.e. coculture system containing human amnion-derived mesenchymal stem cells (HAMSCs) and human bone marrow mesenchymal stem cells (HBMSCs). Two in vitro coculture models, transwell and mixed cocultures, were proposed for 7 days with variable HAMSCs: HBMSCs ratios. Then, supernatant from each coculture was used to reverse the deficiency of HBMSCs and human umbilical vein endothelial cells (HUVECs) impaired by high glucose and palmitic acid (GP)...
September 2018: Biochimie
Michel Aurrand-Lions, Stéphane J C Mancini
After birth, the development of hematopoietic cells occurs in the bone marrow. Hematopoietic differentiation is finely tuned by cell-intrinsic mechanisms and lineage-specific transcription factors. However, it is now clear that the bone marrow microenvironment plays an essential role in the maintenance of hematopoietic stem cells (HSC) and their differentiation into more mature lineages. Mesenchymal and endothelial cells contribute to a protective microenvironment called hematopoietic niches that secrete specific factors and establish a direct contact with developing hematopoietic cells...
August 10, 2018: International Journal of Molecular Sciences
Guido Carpino, Vincenzo Cardinale, Trine Folseraas, Diletta Overi, Krzysztof Grzyb, Daniele Costantini, Pasquale Bartolomeo Berloco, Sabina Di Matteo, Tom Hemming Karlsen, Domenico Alvaro, Eugenio Gaudio
Primary sclerosing cholangitis (PSC) is a chronic inflammatory cholangiopathy frequently complicated by cholangiocarcinoma (CCA). Massive proliferation of biliary tree stem/progenitor cells (BTSCs), expansion of peribiliary glands (PBGs), and dysplasia were observed in PSC. The aims of the present study were to evaluate the involvement of PBGs and BTSCs in CCA which emerged in PSC patients. Specimens from normal liver (N=5), PSC (N=20), and PSC-associated CCA (N=20) were included. Samples were processed for histology, immunohistochemistry and immunofluorescence...
August 13, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Sophie Van Linthout, Ahmed Elsanhoury, Oliver Klein, Marzena Sosnowski, Kapka Miteva, Dirk Lassner, Mohamed Abou-El-Enein, Burkert Pieske, Uwe Kühl, Carsten Tschöpe
AIMS: Myocarditis is often associated with parvovirus B19 (B19V) persistence, which can induce vascular damage. Based on the antiviral and anti-inflammatory properties of telbivudine, we aimed to evaluate its efficacy to protect B19V-infected endothelial cells in vitro and to treat chronic lymphocytic myocarditis patients with B19V transcriptional activity. METHODS AND RESULTS: We evaluated the endothelial-protective potential of telbivudine in human microvascular endothelial cells-1, which were infected with B19V...
August 11, 2018: ESC Heart Failure
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