keyword
https://read.qxmd.com/read/38534600/fibrin-based-hydrogels-with-reactive-amphiphilic-copolymers-for-mechanical-adjustments-allow-for-capillary-formation-in-2d-and-3d-environments
#1
JOURNAL ARTICLE
Svenja Wein, Carina Schemmer, Miriam Aischa Al Enezy-Ulbrich, Shannon Anna Jung, Stephan Rütten, Mark Kühnel, Danny Jonigk, Wilhelm Jahnen-Dechent, Andrij Pich, Sabine Neuss
This study focuses on enhancing controllable fibrin-based hydrogels for tissue engineering, addressing existing weaknesses. By integrating a novel copolymer, we improved the foundation for cell-based angiogenesis with adaptable structural features. Tissue engineering often faces challenges like waste disposal and nutrient supply beyond the 200 µm diffusion limit. Angiogenesis breaks through this limitation, allowing the construction of larger constructs. Our innovative scaffold combination significantly boosts angiogenesis, resulting in longer branches and more capillary network junctions...
March 6, 2024: Gels
https://read.qxmd.com/read/38534403/absence-of-the-klotho-function-causes-cornea-degeneration-with-specific-features-resembling-fuchs-endothelial-corneal-dystrophy-and-bullous-keratopathy
#2
JOURNAL ARTICLE
Chun-Yen Wu, Da-Fong Song, Zhi-Jia Chen, Chao-Sheng Hu, David Pei-Cheng Lin, Han-Hsin Chang
The Klotho loss-of-function mutation is known to cause accelerated senescence in many organs, but its effects on the cornea have not been published. The present study aims to investigate the effects of the Klotho null mutation on cornea degeneration and to characterize the pathological features. Mouse corneas of Klotho homozygous, heterozygous, and wild-type mice at 8 weeks of age for both genders were subject to pathological and immunohistological examinations. The results show an irregular topography on the corneal surface with a Klotho null mutation...
February 20, 2024: Biology
https://read.qxmd.com/read/38534391/generation-of-highly-functional-hepatocyte-like-organoids-from-human-adipose-derived-mesenchymal-stem-cells-cultured-with-endothelial-cells
#3
JOURNAL ARTICLE
Shuhai Chen, Yu Saito, Yuhei Waki, Tetsuya Ikemoto, Hiroki Teraoku, Shinichiro Yamada, Yuji Morine, Mitsuo Shimada
Previously, we successfully established a highly functional, three-dimensional hepatocyte-like cell (3D-HLC) model from adipose-derived mesenchymal stem cells (ADSCs) via a three-step differentiation protocol. The aim of the present study was to investigate whether generating hepatocyte-like organoids (H-organoids) by adding endothelial cells further improved the liver-like functionality of 3D-HLCs and to assess H-organoids' immunogenicity properties. Genes representing liver maturation and function were detected by quantitative reverse transcription-PCR analysis...
March 20, 2024: Cells
https://read.qxmd.com/read/38534357/differentiation-of-adipose-tissue-mesenchymal-stem-cells-into-endothelial-cells-depends-on-fat-depot-conditions-regulation-by-mirna
#4
JOURNAL ARTICLE
Gemma Arderiu, Anna Civit-Urgell, Alberto Díez-Caballero, Fabrizio Moscatiello, Carlos Ballesta, Lina Badimon
The development of obesity is associated with substantial modulation of adipose tissue (AT) structure. The plasticity of the AT is reflected by its remarkable ability to expand or reduce in size throughout the adult lifespan, which is linked to the development of its vasculature. This increase in AT vasculature could be mediated by the differentiation of adipose tissue-derived stem cells (ASCs) into endothelial cells (ECs) and form new microvasculature. We have already shown that microRNA (miRNA)-145 regulates the differentiation of ASCs into EC-like (ECL) cells...
March 14, 2024: Cells
https://read.qxmd.com/read/38534325/indoxyl-sulfate-induced-valve-endothelial-cell-endothelial-to-mesenchymal-transition-and-calcification-in-an-integrin-linked-kinase-dependent-manner
#5
JOURNAL ARTICLE
Maria Delgado-Marin, Sandra Sánchez-Esteban, Alberto Cook-Calvete, Sara Jorquera-Ortega, Carlos Zaragoza, Marta Saura
Calcific Aortic Valve Disease (CAVD) is a significant concern for cardiovascular health and is closely associated with chronic kidney disease (CKD). Aortic valve endothelial cells (VECs) play a significant role in the onset and progression of CAVD. Previous research has suggested that uremic toxins, particularly indoxyl sulfate (IS), induce vascular calcification and endothelial dysfunction, but the effect of IS on valve endothelial cells (VECs) and its contribution to CAVD is unclear. Our results show that IS reduced human VEC viability and increased pro-calcific markers RUNX2 and alkaline phosphatase (ALP) expression...
March 8, 2024: Cells
https://read.qxmd.com/read/38533956/assembled-disassembled-modular-scaffolds-for-multicellular-tissue-engineering
#6
JOURNAL ARTICLE
Xiaopeng Yu, Hongshi Ma, Yufeng Wang, Jianxin Hao, Lei Chen, Michael Gelinsky, Chengtie Wu
The behavior of tissue resident cells can be influenced by the spatial arrangement of cellular interactions. Therefore, it is of significance to precisely control the spatial organization of various cells within multicellular constructs. It remains challenging to construct a versatile multicellular scaffold with ordered spatial organization of multiple cell types. Herein, a modular multicellular tissue engineering scaffold with ordered spatial distribution of different cell types is constructed by assembling varying cell-laden modules...
March 27, 2024: Advanced Materials
https://read.qxmd.com/read/38533089/novel-lipid-mediator-7-s-14-r-docosahexaenoic-acid-biogenesis-and-harnessing-mesenchymal-stem-cells-to-ameliorate-diabetic-mellitus-and-retinal-pericyte-loss
#7
JOURNAL ARTICLE
Yan Lu, Haibin Tian, Hongying Peng, Quansheng Wang, Bruce A Bunnell, Nicolas G Bazan, Song Hong
Introduction: Stem cells can be used to treat diabetic mellitus and complications. ω3-docosahexaenoic acid (DHA) derived lipid mediators are inflammation-resolving and protective. This study found novel DHA-derived 7 S ,14 R -dihydroxy-4 Z ,8 E ,10 Z ,12 E ,16 Z ,19 Z -docosahexaenoic acid (7 S ,14 R -diHDHA), a maresin-1 stereoisomer biosynthesized by leukocytes and related enzymes. Moreover, 7 S ,14 R -diHDHA can enhance mesenchymal stem cell (MSC) functions in the amelioration of diabetic mellitus and retinal pericyte loss in diabetic db/db mice...
2024: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/38532893/bms-794833-reduces-anlotinib-resistance-in-osteosarcoma-by-targeting-the-vegfr-ras-cdk2-pathway
#8
JOURNAL ARTICLE
Qingtao Meng, Jian Han, Peng Wang, Chenxu Jia, Mingyang Guan, Bolun Zhang, Wenzhi Zhao
BACKGROUND: Osteosarcoma, a tumor that originates from bone cells, has a poor prognosis and a high degree of malignancy. Anlotinib, a small-molecule multi-target tyrosine kinase inhibitor (TKI), is the first-line drug in treating osteosarcoma, especially in late-stage osteosarcoma. However, patients often develop resistance after using anlotinib for a certain period, which poses a challenge to its further clinical application. Recently, several TKIs, for instance regorafenib and cabozantinib, have showed clinical interest in treating osteosarcoma and target both vascular endothelial growth factor receptor (VEGFR) and mesenchymal epithelial transition factor (c-MET)...
April 2024: Journal of Bone Oncology
https://read.qxmd.com/read/38528688/soy-derived-isoflavones-as-chemo-preventive-agents-targeting-multiple-signalling-pathways-for-cancer-prevention-and-therapy
#9
REVIEW
Agnieszka Kaufman-Szymczyk, Justyna Jalmuzna, Katarzyna Lubecka-Gajewska
The chemopreventive and chemotherapeutic properties of soy and soy-derived compounds, especially isoflavones, have been extensively studied in recent years. However, in contrast to their anticancer effects, such as cell growth inhibition, cell cycle arrest and apoptosis induction, isoflavones have also been found to promote the growth of cancer cells. Therefore, the aim of this comprehensive review article is to present the current state of knowledge regarding the molecular mechanisms by which soy-derived isoflavones target multiple cellular signalling pathways in cancer cells...
March 25, 2024: British Journal of Pharmacology
https://read.qxmd.com/read/38524278/blockage-of-%C3%AE-v%C3%AE-3-integrin-in-3d-culture-of-triple-negative-breast-cancer-and-endothelial-cells-inhibits-migration-and-discourages-endothelial-to-mesenchymal-plasticity
#10
JOURNAL ARTICLE
Bruna Carla Casali, Matheus Pintor Baptista, Bianca Cruz Pachane, Anelise Abreu Cortez, Wanessa Fernanda Altei, Heloísa Sobreiro Selistre-de-Araújo
Breast cancer is a relevant cause of mortality in women and its triple-negative subtype (TNBC) is usually associated with poor prognosis. During tumor progression to metastasis, angiogenesis is triggered by the sprouting of endothelial cells from pre-existing vessels by a dynamic chain of events including VE-cadherin downregulation, actin protrusion, and integrin-mediated adhesion, allowing for migration and proliferation. The binding of tumoral and tumor-associated stromal cells with the extracellular matrix through integrins mediates angiogenic processes and certain integrin subtypes, such as the αv β3 integrin, are upregulated in hypoxic TNBC models...
July 2024: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/38522867/endocardial-fibroelastosis-resection-when-it-works-and-when-it-does-not
#11
REVIEW
Gregor Gierlinger, Sitaram M Emani
Endocardial fibroelastosis (EFE) is a thickening of the endocardial layer by accumulation of collagen and elastic fibers. Endothelial to mesenchymal transformation is proposed to be the underlying mechanism of formation. Although EFE can occur in both right and left ventricles, this article will focus on management of left ventricular EFE. Through its fibrous, nonelastic manifestation EFE restricts the myocardium leading to diastolic and systolic ventricular dysfunction and prevents ventricular growth in neonates and infants...
2024: Seminars in Thoracic and Cardiovascular Surgery. Pediatric Cardiac Surgery Annual
https://read.qxmd.com/read/38522762/blockade-of-endothelial-adenosine-receptor-2a-suppresses-atherosclerosis-in-vivo-through-inhibiting-creb-alk5-mediated-endothelial-to-mesenchymal-transition
#12
JOURNAL ARTICLE
Yongfeng Cai, Yaqi Zhou, Qiuhua Yang, Jiean Xu, Qingen Da, Qian Ma, Dingwei Zhao, Tammy Lu, Ha Won Kim, David Fulton, Xuejun Jiang, Neal L Weintraub, Kunzhe Dong, Suowen Xu, Mei Hong, Zhiping Liu, Yuqing Huo
Cardiovascular diseases (CVDs) are the leading cause of death worldwide, and morbidity and mortality rates continue to rise. Atherosclerosis constitutes the principal etiology of CVDs. Endothelial injury, inflammation, and dysfunction are the initiating factors of atherosclerosis. Recently, we reported that endothelial adenosine receptor 2A (ADORA2A), a G protein-coupled receptor (GPCR), plays critical roles in neovascularization disease and cerebrovascular disease. However, the precise role of endothelial ADORA2A in atherosclerosis is still not fully understood...
March 22, 2024: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://read.qxmd.com/read/38520399/inhibition-of-nf-%C3%AE%C2%BAb-mediated-proinflammatory-transcription-by-ru-ii-complexes-of-anti-angiogenic-ligands-in-triple-negative-breast-cancer
#13
JOURNAL ARTICLE
Ayan Chakraborty, Shilpendu Ghosh, Manas Pratim Chakraborty, Sujato Mukherjee, Shantanu Saha Roy, Rahul Das, Moulinath Acharya, Arindam Mukherjee
Nuclear factor kappa beta (NF-κB) plays a pivotal role in breast cancer, particularly triple-negative breast cancer, by promoting inflammation, proliferation, epithelial-mesenchymal transition, metastasis, and drug resistance. Upregulation of NF-κB boosts vascular endothelial growth factor (VEGF) expression, assisting angiogenesis. The Ru(II) complexes of methyl- and dimethylpyrazolyl-benzimidazole N , N donors inhibit phosphorylation of ser536 in p65 and translocation of the NF-κB heterodimer (p50/p65) to the nucleus, disabling transcription to upregulate inflammatory signaling...
March 23, 2024: Journal of Medicinal Chemistry
https://read.qxmd.com/read/38519450/tgf%C3%AE-signalling-a-nexus-between-inflammation-placental-health-and-preeclampsia-throughout-pregnancy
#14
JOURNAL ARTICLE
Monika Horvat Mercnik, Carolin Schliefsteiner, Gonzalo Sanchez-Duffhues, Christian Wadsack
BACKGROUND: The placenta is a unique and pivotal organ in reproduction, controlling crucial growth and cell differentiation processes that ensure a successful pregnancy. Placental development is a tightly regulated and dynamic process, in which the transforming growth factor beta (TGFβ) superfamily plays a central role. This family of pleiotropic growth factors is heavily involved in regulating various aspects of reproductive biology, particularly in trophoblast differentiation during the first trimester of pregnancy...
March 22, 2024: Human Reproduction Update
https://read.qxmd.com/read/38519036/intranasal-delivery-of-small-extracellular-vesicles-from-specific-subpopulation-of-mesenchymal-stem-cells-mitigates-traumatic-spinal-cord-injury
#15
JOURNAL ARTICLE
Yi Sun, Jinyun Zhao, Quanbo Liu, Yan Xu, Yiming Qin, Rundong He, Lifu Zheng, Yong Xie, Chengjun Li, Tianding Wu, Yong Cao, Chunyue Duan, Hongbin Lu, Jianzhong Hu
Vascular injury following spinal cord injury (SCI) can significantly exacerbate secondary SCI and result in neurological dysfunction. Strategies targeting angiogenesis have demonstrated potential in enhancing functional recovery post-SCI. In the context of angiogenesis, the CD146+ and CD271+ subpopulations of mesenchymal stem cells (MSCs) have been recognized for their angiogenic capabilities in tissue repair. Small extracellular vesicles (sEVs) derived from MSCs are nanoscale vesicles containing rich bioactive components that play a crucial role in tissue regeneration...
March 20, 2024: Journal of Controlled Release
https://read.qxmd.com/read/38518198/the-pathological-roles-and-potential-mechanisms-of-vascular-endothelial-growth-factor-receptor-3-in-gastric-cancer
#16
JOURNAL ARTICLE
Xiu-Feng Li, Xiu-Juan Zhang, Fu-Rong Hao, Xiao-Tong Dong, Guo-Dong Xu, Yun-Xiang Zhang
OBJECTIVE: To investigate the roles and underlying mechanisms of vascular endothelial growth factor receptor-3 (VEGFR-3) in gastric cancer (GC). METHODS: VEGFR-3 gene expression profiles in human gastric adenocarcinoma (GAC) tissues were analysed using The Cancer Genome Atlas database. Human GC cell lines and were used for in vitro studies. Mouse models of GC and distant metastasis were used for in vivo studies. Silencing of VEGFR-3 gene expression was achieved using small interfering RNA...
March 2024: Journal of International Medical Research
https://read.qxmd.com/read/38516693/endovascular-transplantation-of-mrna-enhanced-mesenchymal-stromal-cells-results-in-superior-therapeutic-protein-expression-in-swine-heart
#17
JOURNAL ARTICLE
Jonathan Al-Saadi, Mathias Waldén, Mikael Sandell, Jesper Sohlmér, Rikard Grankvist, Ida Friberger, Agneta Andersson, Mattias Carlsten, Kenneth Chien, Johan Lundberg, Nevin Witman, Staffan Holmin
Heart failure has a poor prognosis and no curative treatment exists. Clinical trials are investigating gene- and cell-based therapies to improve cardiac function. The safe and efficient delivery of these therapies to solid organs is challenging. Herein, we demonstrate the feasibility of using an endovascular intramyocardial delivery approach to safely administer mRNA drug products and perform cell transplantation procedures in swine. Using a trans -vessel wall (TW) device, we delivered chemically modified mRNAs (modRNA) and mRNA-enhanced mesenchymal stromal cells expressing vascular endothelial growth factor A (VEGF-A) directly to the heart...
June 13, 2024: Molecular Therapy. Methods & Clinical Development
https://read.qxmd.com/read/38515582/extracellular-vesicles-derived-from-sars-cov-2-m-protein-induced-triple-negative-breast-cancer-cells-promoted-the-ability-of-tissue-stem-cells-supporting-cancer-progression
#18
JOURNAL ARTICLE
Hoai-Nga Thi Nguyen, Cat-Khanh Vuong, Mizuho Fukushige, Momoko Usuda, Liora Kaho Takagi, Toshiharu Yamashita, Mana Obata-Yasuoka, Hiromi Hamada, Motoo Osaka, Toru Tsukada, Yuji Hiramatsu, Osamu Ohneda
INTRODUCTION: SARS-CoV-2 infection increases the risk of worse outcomes in cancer patients, including those with breast cancer. Our previous study reported that the SARS-CoV-2 membrane protein (M-protein) promotes the malignant transformation of triple-negative breast cancer cells (triple-negative BCC). METHODS: In the present study, the effects of M-protein on the ability of extracellular vesicles (EV) derived from triple-negative BCC to regulate the functions of tissue stem cells facilitating the tumor microenvironment were examined...
2024: Frontiers in Oncology
https://read.qxmd.com/read/38509522/overexpression-of-wnt5a-promoted-the-protective-effect-of-mesenchymal-stem-cells-on-lipopolysaccharide-induced-endothelial-cell-injury-via-activating-pi3k-akt-signaling-pathway
#19
JOURNAL ARTICLE
Manliang Guo, Shiqi Li, Chuan Li, Xueyan Mao, Liru Tian, Xintong Yang, Caixia Xu, Mian Zeng
BACKGROUND: Lung endothelial barrier injury plays an important role in the pathophysiology of acute lung injury/acute respiratory distress syndrome (ALI/ARDS). Mesenchymal stem cells (MSCs) therapy has shown promise in ARDS treatment and restoration of the impaired barrier function. It has been reported that Wnt5a shows protective effects on endothelial cells. Therefore, the study aimed to investigate whether overexpression of Wnt5a could promote the protective effects of MSCs on Lipopolysaccharide (LPS)-induced endothelial cell injury...
March 20, 2024: BMC Infectious Diseases
https://read.qxmd.com/read/38508181/single-cell-omics-identifies-inflammatory-signaling-as-a-trans-differentiation-trigger-in-mouse-embryos
#20
JOURNAL ARTICLE
Yifan Zhang, Zhixin Kang, Mengyao Liu, Lu Wang, Feng Liu
Trans-differentiation represents a direct lineage conversion; however, insufficient characterization of this process hinders its potential applications. Here, to explore a potential universal principal for trans-differentiation, we performed single-cell transcriptomic analysis of endothelial-to-hematopoietic transition (EHT), endothelial-to-mesenchymal transition, and epithelial-to-mesenchymal transition in mouse embryos. We applied three scoring indexes of entropies, cell-type signature transcription factor expression, and critical transition signals to show common features underpinning the fate plasticity of transition states...
March 18, 2024: Developmental Cell
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