Xinlei Wei, Xue Yang, Congcong Hu, Qiangzi Li, Qianqian Liu, Yue Wu, Leipeng Xie, Xiao Ning, Fei Li, Tao Cai, Zhiguang Zhu, Yi-Heng P Job Zhang, Yanfei Zhang, Xuejun Chen, Chun You
In vitro biotransformation (ivBT) facilitated by in vitro synthetic enzymatic biosystems (ivSEBs) has emerged as a highly promising biosynthetic platform. Several ivSEBs have been constructed to produce poly-3-hydroxybutyrate (PHB) via acetyl-coenzyme A (acetyl-CoA). However, some systems are hindered by their reliance on costly ATP, limiting their practicality. This study presents the design of an ATP-free ivSEB for one-pot PHB biosynthesis via acetyl-CoA utilizing starch-derived maltodextrin as the sole substrate...
April 16, 2024: Nature Communications