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Wenhui Ma, Zhuoluo Xu, Yutian Wang, Wenyi Li, Zhigang Wei, Tao Chen, Tingyu Mou, Mingzhen Cheng, Jun Luo, Tingyue Luo, Yuehong Chen, Jiang Yu, Weijie Zhou, Guoxin Li
Rationale: This study is to validate the clinicopathologic significance and potential prognostic value of SLP2 in gastric cancer (GC), to investigate the biological function and regulation mechanism of SLP2, and to explore potential therapeutic strategies for GC. Methods: The expression of SLP2 in GC tissues from two cohorts was examined by IHC. The biological function and regulation mechanism of SLP2 and PHB was validated via loss-of-function or gain-of-function experiments. In vitro proliferation detection was used to evaluate the therapeutic effects of Sorafenib...
2018: Theranostics
Tatiane K Furuya, Carlos E Jacob, Michele T P Tomitão, Lizeth C C Camacho, Marcus F K P Ramos, José Eluf-Neto, Venâncio A F Alves, Bruno Zilberstein, Ivan Cecconello, Ulysses Ribeiro, Roger Chammas
The chronic inflammatory microenvironment and immune cell dysfunction have been described as critical components for gastric tumor initiation and progression. The diffuse subtype is related to poor clinical outcomes, pronounced inflammation, and the worst prognosis. We investigated the association of polymorphisms in inflammatory response-related genes ( COX-2 , OGG1 , TNFB , TNFA , HSPA1L , HSPA1B , VEGFA , IL17F , LGALS3 , PHB , and TP53 ) with gastric cancer susceptibility, progression and prognosis in a Brazilian sample, focusing on the diffuse subtype...
December 13, 2018: Genes
Sergio Bordel, Elisa Rodríguez, Raúl Muñoz
Polyhydroxyalkanoates (PHAs) are biodegradable plastics that can be produced by some methanotrophic organisms such as those of the genus Methylocystis. This allows the conversion of a detrimental greenhouse gas into an environmentally friendly high added-value bioproduct. This study presents the genome sequence of Methylocystis hirsuta CSC1 (a high yield PHB producer). The genome comprises 4,213,043 bp in 4 contigs, with the largest contig being 3,776,027 bp long. Two of the other contigs are likely to correspond to large size plasmids...
December 12, 2018: MicrobiologyOpen
Nicole Remaliah Samantha Sibuyi, Mervin Meyer, Martin Opiyo Onani, Amanda Skepu, Abram Madimabe Madiehe
Obesity is a global epidemic that poses a serious health concern due to it being a risk factor for life-threatening chronic diseases, such as type 2 diabetes, cancer, and cardiovascular diseases. Pharmacotherapy remains the mainstay for the management of obesity; however, its usefulness is limited due to poor drug efficacy, non-specificity and toxic side effects. Therefore, novel approaches that could provide insights into obesity and obesity-associated diseases as well as development of novel anti-obesity treatment modalities or improvement on the existing drugs are necessary...
2018: International Journal of Nanomedicine
Chen Ling, Guan-Qing Qiao, Bo-Wen Shuai, Kun-Nan Song, Wen-Xi Yao, Xiao-Ran Jiang, Guo-Qiang Chen
Halomonas has been developed as a platform for the next generation industrial biotechnology (NGIB) allowing open and non-sterile growth without microbial contamination under a high salt concentration and alkali pH. To reduce downstream cost associated with continuous centrifugation and salt containing wastewater treatment, Halomonas campaniensis strain LS21 was engineered to become self-flocculating by knocking out an etf operon encoding two subunits of an electron transferring flavoprotein in the predicted electron transfer chain...
December 8, 2018: Biotechnology and Bioengineering
Fahimeh Zeraatkar Kashani, Sayed Mehdi Ghoreishi, Asma Khoobi, Morteza Enhessari
An electrochemical sensor is described for the simultaneous determination of ortho-hydroxybenzoic acid (OHB) and para-hydroxybenzoic acid (PHB). The sensor consists of a carbon paste electrode modified with nickel titanate nanoceramics (NiTiO3 /CPE). The NiTiO3 nanoceramics and the nanostructured modified CPE were characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, electrochemical impedance spectroscopy and cyclic voltammetry. Differential pulse voltammetry indicates that the response to OHB (best measured at 0...
December 10, 2018: Mikrochimica Acta
Chaozhi Pan, Giin-Yu Amy Tan, Liya Ge, Chia-Lung Chen, Jing-Yuan Wang
With increasing demand for biodiesel, crude glycerol as a by-product in biodiesel production has been generated and oversupplied. This study, therefore, explored the pretreatment and a subsequent two-stage microbial system to convert crude glycerol into high value-added products: 1,3-propanediol (1,3-PDO) and polyhydroxyalkanoates (PHAs). After pretreatment, long chain fatty acids (LCFAs) could be effectively removed from crude glycerol to eliminate the inhibition effects on subsequent microbial process. In the anaerobic fermentation, when fed treated crude glycerol increased from 20 g/L to 100 g/L, 1,3-PDO yield decreased from 0...
December 3, 2018: Journal of Environmental Management
Blanca Hernando-Rodríguez, Marta Artal-Sanz
Mitochondrial functions are essential for life, critical for development, maintenance of stem cells, adaptation to physiological changes, responses to stress, and aging. The complexity of mitochondrial biogenesis requires coordinated nuclear and mitochondrial gene expression, owing to the need of stoichiometrically assemble the oxidative phosphorylation (OXPHOS) system for ATP production. It requires, in addition, the import of a large number of proteins from the cytosol to keep optimal mitochondrial function and metabolism...
November 29, 2018: Cells
Zahra Montazer, Mohammad B Habibi Najafi, David B Levin
We have characterized the ability of eight bacterial strains to utilize powdered low-density polyethylene (LDPE) plastic (un-treated and without any additives) as a sole carbon source. Cell mass production on LDPE containing medium after 21 days of incubation varied between 0.083 ± 0.015 g L-1 cell dry weight (cdw) for Micrococcus luteus IRN20 and 0.39 ± 0.036 g L-1 for Cupriavidus necator H16. The percent decrease in LDPE mass ranged from 18.9% ± 0.72 for M. luteus IRN20 to 33.7% ± 1.2 for C. necator H16...
November 28, 2018: Canadian Journal of Microbiology
Eva Petrovova, Maria Giretova, Alena Kvasilova, Oldrich Benada, Jan Danko, Lubomir Medvecky, David Sedmera
Using scaffolds with appropriate porosity represents a potential approach for repair of critical-size bone defects. Vascularization is essential for bone formation and healing. This study investigates methods for monitoring angiogenesis within porous biopolymer scaffolds on the basis of polyhydroxybutyrate (PHB)/chitosan. We use the chick and quail chorioallantoic membrane (CAM) assay as an in vivo model focused on the formation of new blood vessels inside the implant structure. Chemical properties of the surface in biopolymer scaffold matrix were characterized as well as the tissue reaction of the CAM...
November 23, 2018: ALTEX
Donya Kamravamanesh, Christoph Slouka, Andreas Limbeck, Maximilian Lackner, Christoph Herwig
Recently, several mutants of Synechocystis sp. PCC 6714 were obtained showing superior PHB content and productivities. Here, the most promising mutant named MT_a24 is compared in detail with the wild-type in controlled photobioreactors. In order to provide an easily scalable and alternative approach to the normally done two-step process -comprising of growth phase and limitation phase- a one-step cultivation was optimized. The multivariate experimental design approach was used for the optimization of the one-step, self-limiting media...
November 9, 2018: Bioresource Technology
Jennifer Paola Florez, Mahyar Fazeli, Renata Antoun Simão
The present work aims to study the effect of the addition of poly (hydroxybutyrate) PHB as reinforcement in thermoplastic cornstarch (TPS) produced by the thermal compression molding method. Initially, the physical and chemical properties of TPS with different amounts of glycerol as the plasticizer (25, 30 and 35 wt%), are evaluated. Then, the composites, including 35% glycerol and different PHB contents (10, 20 and 30 wt%), are prepared. Additionally, the surface of the PHB granules is modified by plasma treatment using atmospheric air and sulfur hexafluoride (SF6 ) gas...
November 14, 2018: International Journal of Biological Macromolecules
Xiaohua Liu, Xiaoming Feng
A highly regioselective hydroalkenylation of imines or aldehydes with styrene derivatives was realized by dual catalysis with Ni(cod)2 /PCy3 and either TsNH2 or PhB(OH)2 . This process provides an atom-, step-, and redox-economic pathway towards synthetically useful allylic amines and alcohols, and opens up a new avenue for the design of more versatile coupling reactions.
November 15, 2018: Angewandte Chemie
Constanze Nossol, Peter Landgraf, Stefan Kahlert, Michael Oster, Berend Isermann, Daniela C Dieterich, Klaus Wimmers, Sven Dänicke, Hermann-Josef Rothkötter
Deoxynivalenol (DON) is a toxin found in cereals as well as in processed products such as pasta, and causes substantial economic losses for stock breeding as it induces vomiting, reduced feeding, and reduced growth rates in piglets. Oxidative phosphorylation, TCA-cycle, transcription, and translation have been hypothesized to be leading pathways that are affected by DON. We used an application of high and low glucose to examine oxidative phosphorylation and anaerobic glycolysis. A change in the metabolic status of IPEC-J2 was observed and confirmed by microarray data...
November 9, 2018: Toxins
Fahimeh Zeraatkar Kashani, Sayed Mehdi Ghoreishi, Asma Khoobi
In this research, differential pulse voltammetry (DPV) coupled with experimental design, was used for determination of p-hydroxybenzoic acid (PHB) in cosmetics. Optimization of effecting parameters was carried out based on rotatable central composite design (RCCD) and response surface methodology (RSM) at the surface of a nanostructured electrode for achieving the best sensitivity. Sol-gel process was used for synthesize of nickel titanate (NiTiO3 ) nanoceramics. The structural and morphological characterization of the nanoparticles was studied by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM)...
January 1, 2019: Materials Science & Engineering. C, Materials for Biological Applications
Mohamed A Abdelwahab, Ahmed A El-Barbary, Karim S El-Said, Sabry A El Naggar, Hazem M ElKholy
The chemical modification of biodegradable poly(3-hydroxybutyrate) (PHB) is useful for biomedical applications. In this study, the transesterification reaction of PHB was carried out under reflux conditions in the presence of 1,4-butanediol to form telechelic PHB-diol. Further modification of PHB-diol into PHB-diacrylate was carried out by the reaction of PHB-diol with acryloyl chloride. PHB-diacrylate was grafted with amino compounds such as 1,4-butanediamine, 1,3-propanediamine, 1,2-ethylenediamine, piperazine, cyclohexylamine, 2,2'-(ethane-1,2-diylbis(oxy)) diethanamine (jeffamine EDR 148) and morpholine via Michael-type addition reaction...
October 25, 2018: International Journal of Biological Macromolecules
Katie A Wilson, Josh L Garden, Natasha T Wetmore, Stacey D Wetmore
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone is a potent carcinogen found in all tobacco products that leads to a variety of DNA lesions in cells, including O6-[4-oxo-4-(3-pyridyl)butyl]guanine (POB-G) and O6-[4-hydroxy-4-(3-pyridyl)butyl]guanine (PHB-G), which differ by only a single substituent in the bulky moiety. This work uses a multiscale computational approach to shed light on the intrinsic conformational and base-pairing preferences of POB-G and PHB-G, and the corresponding properties in DNA and the polymerase η active site...
November 8, 2018: Nucleic Acids Research
Péter Polyák, Eszter Urbán, Gergely N Nagy, Beáta G Vértessy, Béla Pukánszky
The enzyme catalyzed degradation of poly(3-hydroxybutyrate) (PHB) is a two-step process consisting of the adsorption of the enzyme on the surface of a PHB substrate and the cleavage of ester bonds. A deactivated enzyme was prepared by point mutagenesis to separate the two steps from each other. Measurements carried out with active and inactive enzymes on PHB particles proved that mutagenesis was successful and the modified enzyme did not catalyze degradation. Based on the Michaelis-Menten approach a kinetic model was proposed which could describe the processes quantitatively, the agreement between prediction and the measured data was excellent...
January 2019: Enzyme and Microbial Technology
Ling Leng, Masaru K Nobu, Takashi Narihiro, Peixian Yang, Giin-Yu Amy Tan, Po-Heng Lee
Glycerol is presently being generated in surplus with the rapid growth of the biodiesel industry and seeks ways to be upcycled, rather than to be treated with costs. Glycerol for the co-production of 1,3-propanediol (1,3-PDO) and caproate has a great prospect. Yet, its technical difficulty lies in the enhancement of caproate productivity, which requires the presence of ethanol as a co-substrate and necessitates the co-existence of functional microbes for glycerol fermentation and chain elongation. This study successfully achieved 6...
October 24, 2018: Water Research
Salima Lakehal, Aicha Lakehal, Malika Bouchagour, Christophe Morell, Henry Chermette
In this work, the nature of the chemical interactions between the metalloid atom (M = Si, Ge, As, Sb, Te, Po) and the nitrogen atoms in the bora-amidinate (bam) complexes (Cln M[PhB(Nt Bu)2 ]) are investigated, mainly via density-based indices. The descriptors used are derived using the quantum theory of atoms in molecules and natural orbitals for chemical valence approaches. It is shown that the strongest interaction is achieved with silicon. Indeed, it is generally the lightest metalloid in a particular group of the periodic table (i...
October 29, 2018: Journal of Molecular Modeling
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