Michael T Patterson, Maria M Firulyova, Yingzheng Xu, Hannah Hillman, Courtney Bishop, Alisha Zhu, Grant H Hickok, Patricia R Schrank, Christine E Ronayne, Zakariya Caillot, Gavin Fredrickson, Ainsley E Kennedy, Nisha Acharya, Jaap G Neels, Giulia Chinetti, Xavier Revelo, Ingunn M Stromnes, Stoyan Ivanov, Tyler D Bold, Konstantin Zaitsev, Jesse W Williams
Atherosclerosis is driven by the expansion of cholesterol-loaded 'foamy' macrophages in the arterial intima. Factors regulating foamy macrophage differentiation and survival in plaque remain poorly understood. Here we show, using trajectory analysis of integrated single-cell RNA sequencing data and a genome-wide CRISPR screen, that triggering receptor expressed on myeloid cells 2 (Trem2) is associated with foamy macrophage specification. Loss of Trem2 led to a reduced ability of foamy macrophages to take up oxidized low-density lipoprotein (oxLDL)...
November 2023: Nat Cardiovasc Res