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mesenchymal stem cells, stem cells,

Tim Kornfeld, Peter M Vogt, Christine Radtke
Artificial and non-artificial nerve grafts are the gold standard in peripheral nerve reconstruction in cases with extensive loss of nerve tissue, particularly where a direct end-to-end suture or an autologous nerve graft is inauspicious. Different materials are marketed and approved by the US Food and Drug Administration (FDA) for peripheral nerve graft reconstruction. The most frequently used materials are collagen and poly(DL-lactide-ε-caprolactone). Only one human nerve allograft is listed for peripheral nerve reconstruction by the FDA...
December 13, 2018: Wiener Medizinische Wochenschrift
Valentina Fonsato, Michela De Lena, Stefania Tritta, Alessia Brossa, Ruggero Calvetti, Ciro Tetta, Giovanni Camussi, Benedetta Bussolati
It is well recognized that Cancer Stem Cells (CSCs) sustain the initiation, the maintenance and the recurrence of tumors. We previously reported that extracellular vesicles (EVs) derived from human liver stem cells (HLSCs) were able to limit tumor development. In this study, we evaluated whether EV derived from HLSCs could act in synergy with tyrosine kinase inhibitors (TKIs) on apoptosis of CSCs isolated from renal carcinomas. For this purpose, we administered to renal CSCs, HLSC-EVs and TKIs, as co-incubation or sequential administration...
November 16, 2018: Oncotarget
Nannan Yan, Jie Xu, Chuanxiang Zhao, Yi Wu, Fengwei Gao, Ci Li, Wenhui Zhou, Tengfei Xiao, Xiaoming Zhou, Qixiang Shao, Sheng Xia
Food allergy prevalence has steadily increased worldwide over the past decades and immunotherapeutic treatment strategies are gaining attention. Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) exhibit similar immune regulatory properties to bone marrow-derived MSCs. hUC-MCSs can be prepared with fewer ethical constraints and are potential candidates for allergic disorder therapies. The current study aimed to investigate potential antiallergic properties of hUC-MSCs in mice with ovalbumin (OVA)-induced food allergy...
December 2018: Experimental and Therapeutic Medicine
Alissa C Greenwald, Tamar Licht, Saran Kumar, Sunday S Oladipupo, Seema Iyer, Myriam Grunewald, Eli Keshet
Insufficient erythropoiesis due to increased demand is usually met by hypoxia-driven up-regulation of erythropoietin (Epo). Here, we uncovered vascular endothelial growth factor (VEGF) as a novel inducer of Epo capable of increasing circulating Epo under normoxic, nonanemic conditions in a previously unrecognized reservoir of Epo-producing cells (EPCs), leading to expansion of the erythroid progenitor pool and robust splenic erythropoiesis. Epo induction by VEGF occurs in kidney, liver, and spleen in a population of Gli1+ SMA+ PDGFRβ+ cells, a signature shared with vascular smooth muscle cells (VSMCs) derived from mesenchymal stem cell-like progenitors...
December 13, 2018: Journal of Experimental Medicine
R A Costa, R S T Martins, E Capilla, L Anjos, D M Power
BACKGROUND: Osteoglycin (OGN, a.k.a. mimecan) belongs to cluster III of the small leucine-rich proteoglycans (SLRP) of the extracellular matrix (ECM). In vertebrates OGN is a characteristic ECM protein of bone. In the present study we explore the evolution of SLRP III and OGN in teleosts that have a skeleton adapted to an aquatic environment. RESULTS: The SLRP gene family has been conserved since the separation of chondrichthyes and osteichthyes. Few gene duplicates of the SLRP III family exist even in the teleosts that experienced a specific whole genome duplication...
December 13, 2018: BMC Evolutionary Biology
Eun-Bin Bae, Ji-Hyun Yoo, Sung-In Jeong, Min-Su Kim, Youn-Mook Lim, Jong-Ju Ahn, Jin-Ju Lee, So-Hyoun Lee, Hyung-Joon Kim, Jung-Bo Huh
This study aimed to evaluate the titanium (Ti) implants coated with collagen type Ⅰ crosslinked using gamma-irrigation or glutaraldehyde (GA). The in vitro surface observations, quantification assay, and cell studies using human mesenchymal stem cells (hMSCs) were conducted. For in vivo experiments, the implants were divided into three groups and inserted into the rat tibias: control group (non-treated Ti implant), GA group (Ti implants coated with GA-crosslinked collagen) and 25 kGy group (Ti implants coated with gamma-radiation-crosslinked collagen at dose of 25 kGy)...
December 11, 2018: Materials
Seon Young Bak, Sang Woo Lee, Chong Hyuk Choi, Hyun Woo Kim
Various methods for isolation of type I collagen using acids, bases, enzymes, and their combinations have been applied. However, a lack of standardization exists among type I collagens isolated by various approaches. Consequently, in this study, we assessed the influence of acetic acid residue on type I collagen isolated by pepsin-acetic acid treatment, the fabrication of collagen-based porous scaffolds, and the seeded cells on collagen scaffolds. Unlike the isolated collagen dialyzed by deionized water (DDW), collagen dialyzed by 0...
December 11, 2018: Materials
Sanja Stojanović, Stevo Najman, Aleksandra Korać
Lipomas are benign adipose tissue tumors of unknown etiology, which can vary in size, number, body localization and cell populations within the tissue. Lipoma-derived stem cells (LDSCs) are proposed as a potential tool in regenerative medicine and tissue engineering due to their similar characteristics with adipose-derived stem cells (ADSCs) reported so far. Our study is among the first giving detailed insights into the molecular signature and differences in the differentiation capacity of LDSCs in vitro compared to ADSCs...
December 8, 2018: Cells
Enrico Pierluigi Spugnini, Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni, Stefano Fais
Metastatic diffusion is thought to be a multi-step phenomenon involving the release of cells from the primary tumor and their diffusion through the body. Currently, several hypotheses have been put forward in order to explain the origin of cancer metastasis, including epithelial⁻mesenchymal transition, mutagenesis of stem cells, and a facilitating role of macrophages, involving, for example, transformation or fusion hybridization with neoplastic cells. In this paradigm, tumor-secreted extracellular vesicles (EVs), such as exosomes, play a pivotal role in cell communications, delivering a plethora of biomolecules including proteins, lipids, and nucleic acids...
December 10, 2018: International Journal of Molecular Sciences
Yuhao Deng, Guanghua Lei, Zixuan Lin, Yuanheng Yang, Hang Lin, Rocky S Tuan
Mesenchymal stem cells (MSCs) represent a promising cell source to regenerate articular cartilage, but current chondroinduction protocols, commonly using transforming growth factor-β (TGFβ), lead to concomitant chondrocytic hypertrophy with ossification risk. Here, we showed that a 14-day culture of MSC-laden hyaluronic acid hydrogel in the presence of TGFβ, followed by 7 days culture in TGFβ-free medium, with the supplement of Wnt/β-catenin inhibitor XAV939 from day 10-21, resulted in significantly reduced hypertrophy phenotype...
November 30, 2018: Biomaterials
Khaled F Al-Massri, Lamiaa A Ahmed, Hanan S El-Abhar
Peripheral neuropathy is a common adverse effect observed during use of paclitaxel (PTX) as chemotherapy. The present investigation was directed to estimate the modulatory effect of bone marrow derived mesenchymal stem cells (BM-MSCs) on pregabalin (PGB) treatment in PTX-induced peripheral neuropathy. Neuropathic pain was induced in rats by injecting PTX (2 mg/kg, i.p) 4 times every other day. Rats were then treated with PGB (30 mg/kg/day, p.o.) for 21 days with or without a single intravenous administration of BM-MSCs...
December 10, 2018: Behavioural Brain Research
Mahdieh Rezaei, Shahram Jamshidi, Anna Saffarpour, Mahdi Ashouri, Reza Rahbarghazi, Amir Reza Rokn, Pouriya Motahari
Appropriate regeneration of periodontal tissues is the primary purpose of periodontal disease treatment. The present study assessed the impact of three key regenerative elements-platelet-rich plasma (PRP), canine bone marrow mesenchymal stem cells (cBM-MSCs), and fibrin glue-on periodontal regeneration. In each of the study's five dogs, Class II furcation defects were established on the buccal surface of five teeth. One tooth (five total) from each dog was placed into one of five groups: (1) PRP + fibrin glue, (2) PRP + fibrin glue + cBM-MSCs, (3) fibrin glue, (4) cBM-MSCs + fibrin glue, and (5) control (no treatment)...
January 2019: International Journal of Periodontics & Restorative Dentistry
Xiao-Xiao Lu, Shao-Zhen Zhao
BACKGROUND: As one of the main blinding ocular diseases, corneal blindness results from neovascularization that disrupts the angiogenic privilege of corneal avascularity. Following neovascularization, inflammatory cells are infiltrating into corneal to strengthen corneal injury. How to maintain corneal angiogenic privilege to treat corneal disease has been investigated for decades. MATERIALS & METHODS: Local administration of viral and non-viral-mediated anti-angiogenic factors reduces angiogenic protein expression in situ with limited or free of off-target effects upon gene delivery...
December 13, 2018: Current Gene Therapy
Taha Bartu Hayal, Binnur Kıratlı, Hatice Burcu Şişli, Fikrettin Şahin, Ayşegül Doğan
Mesenchymal Stem Cells (MSCs) are adult stem cells; isolated from various body parts including bone marrow, adipose tissue and dental tissue, have been characterized well and used in regenerative medicine applications. The promising potential of MSCs makes them great candidates in many disorders. It has been well known in the literature that MSCs interact with cancer cells and regulate the carcinogenesis process at different stages. The dual role of MSCs in cancer progression should be clearly identified at the physiological and molecular level to identify clinical potential in cancer treatment...
December 13, 2018: Advances in Experimental Medicine and Biology
Sefei Yang, Jun Guo, Lei Zhou, Helin Xing, Xianli Wang, Chaofang Dong
MicroRNAs (miRNAs/miRs) have key roles in various physiological and pathological processes by regulating the expression of specific genes. The identification of miRNAs involved in bone metabolism may provide insight into the expression of genes associated with the development of alveolar ridge atrophy. In the present study, the miRNA expression profiles in alveolar ridge atrophy and normal tissue samples were investigated by miRNA microarray analysis. Among the 52 differentially expressed miRNAs identified, the expression levels of 20 selected miRNAs in the alveolar ridge atrophy and normal tissue samples were verified by reverse transcription-quantitative polymerase chain reaction...
December 2018: Experimental and Therapeutic Medicine
Xiaoyi Wang, Zongsheng Zhao, Hongwei Zhang, Jixue Hou, Wenlei Feng, Meng Zhang, Jun Guo, Jie Xia, Quanhu Ge, Xueling Chen, Xiangwei Wu
Mesenchymal stem or stromal cells (MSCs) are identified as sources of pluripotent stem cells with varying degrees of plasticity. Endothelial progenitor cells (EPCs) originate from either bone marrow (BM) or peripheral blood and can mature into cells that line the lumen of blood vessels. MSC and EPC therapies exhibit promising results in a variety of diseases. The current study described the simultaneous isolation of EPCs and MSCs from murine BM using a straightforward approach. The method is based on differences in attachment time and trypsin sensitivity of MSCs and EPCs...
December 2018: Experimental and Therapeutic Medicine
Dong Wang, Zhen-Ling Ji, Jing-Min Wang, Yu-Yan Tan
The objective of this study was to investigate the curative effects of mesenchymal stem cells' tenogenic differentiation on linea alba incision healing induced by bone morphogenetic protein-12. Mesenchymal stem cells were isolated and induced by 10 ng/ml of bone morphogenetic protein-12 for 48 h. Expression of scleraxis, collagen I and collagen III were examined at 48 h, 5 and 7 days to investigate the tenogenic differentiation. The expression of scleraxis increases continually even in the absence of bone morphogenetic protein-12 for 5 days (P<0...
December 2018: Experimental and Therapeutic Medicine
Yan Wei, Zhaohong Xie, Jianzhong Bi, Zhengyu Zhu
Anti-inflammatory effects of bone marrow mesenchymal stem cells (BMSCs) on mice with Alzheimer's disease (AD) were investigated. Twenty amyloid precursor protein (APP)/presenilin-1 (PS1) double transgenic mice were randomly divided into two groups: the AD control group and the stem cell treatment group. The normal control group consisted of 10 non-transgenic mice. The stem cell treatment group was injected with BMSCs, and the two control groups were given the same volume of normal saline. The Morris water maze test was used to compare the memory function of mice, and the relative expression levels of β-site APP cleaving enzyme 1 (BACE1) and α-2-macroglobulin (A2M) genes were detected by fluorescence quantitative polymerase chain reaction (qPCR)...
December 2018: Experimental and Therapeutic Medicine
Xinwei Liu, Mingchang Du, Yu Wang, Songbo Liu, Xianmin Liu
Osteoarthritis (OS) is a common disease in orthopedics. Although OS is known as an inflammation mediated by inflammatory cytokines; however, the mechanism is poorly understood. In the present study, the role of bone morphogenetic protein-9 (BMP9) was investigated in chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs). ADMSCs were transfected with BMP9. BMP9 mRNA expression was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Type II collagen and aggrecan expression was detected by western blotting and RT-qPCR...
December 2018: Experimental and Therapeutic Medicine
Jiadi Gan, Yingjin Wang, Xiaodong Zhou
The efficacy of stem cell (SC) transplantation in patients with type 1 diabetes mellitus (T1DM) has remained to be fully elucidated. In the present study, a systematic review and meta-analysis was performed to determine the clinical outcomes. Electronic databases, including PubMed, MEDLINE, WanFang and the Cochrane Library were screened for relevant studies published until January 13, 2018. The references of retrieved papers, systematic reviews and trial registries were manually screened for additional papers...
December 2018: Experimental and Therapeutic Medicine
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