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mesenchymal stem cells, stem cells,

Zhenjie Sun, Liping Ouyang, Xiaohan Ma, Yuqin Qiao, Xuanyong Liu
Polyetheretherketone (PEEK) is ideal for dental and orthopedic applications because its mechanical properties are similar to cortical bones. However, its inherent inert ability hinders its clinical applications. In this work, bone morphogenetic protein-2 (BMP-2) was immobilized onto the sulfonated PEEK (SPEEK) using lyophilization technology. The surface morphologies of the samples were analyzed by field-emission scanning electron microscopy (FE-SEM), and the chemical compositions were analyzed by energy-dispersive X-ray spectrometry (EDS)...
August 8, 2018: Colloids and Surfaces. B, Biointerfaces
Le Huang, Xinluan Wang, Huijuan Cao, Ling Li, Dick Ho-Kiu Chow, Li Tian, Heng Wu, Jiayong Zhang, Nan Wang, Lizhen Zheng, Xinsheng Yao, Zhijun Yang, Ling Qin
Targeting delivery system has been widely used in packaging drugs for medical therapies attributed to its high efficiency and efficacy. A Traditional Chinese Medicine (TCM) formula consisting of Herba Epimedii has previously been shown to effectively treat postmenopausal osteoporosis. We have subsequently found that icaritin, which was a flavonoid isolated from both Herba Epimedii and its serum metabolites after oral administration, inhibited the adipogenic capacity of bone mesenchymal stem cells (BMSCs) while promoted their osteogenesis...
August 6, 2018: Biomaterials
Eun-Jung Kim, Kyung-Sik Yoon, Makiko Arakaki, Keishi Otsu, Satoshi Fukumoto, Hidemitsu Harada, David William Green, Jong-Min Lee, Han-Sung Jung
BACKGROUND: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use iPSCs. However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. RESULTS: Here, we tried to induce differentiation methods for dental epithelial cells and dental mesenchymal cells from iPSCs...
August 14, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
Limei Wu, Surya Amarachintha, Jian Xu, Frank Oley, Wei Du
The bone marrow (BM) microenvironment (niche) plays important roles in supporting normal/abnormal haematopoiesis. We investigated the interaction between leukaemic mesenchymal niche and haematopoietic stem and progenitor cells (HSPCs) using the model of Fanconi anaemia (FA), a genetic disorder characterized by BM failure and leukaemia. Healthy donor HSPCs co-cultured on mesenchymal stromal cells (MSCs) derived from FA patients with acute myeloid leukaemia (AML) exhibited higher human engraftment and myeloid expansion in Non-obese diabetic severe combined immunodeficiency IL-2γ-/- /SGM3 recipients...
August 14, 2018: British Journal of Haematology
Tao Fan, Guozhi Huang, Wen Wu, Rong Guo, Qing Zeng
The authors of the above article wish to retract it on account of two reasons: First, the authors have experienced irreconcilable difficulties in deciding upon the suitable order of the authors in the author list. Secondly, due to the authors' negligence, the graph featured in Fig. 1 on p. 1328 does not match with the textual description, and the authors intend to rectify this discrepancy by performing further experiments. The authors regret any inconvenience that this retraction has caused. [the original article was published in Molecular Medicine Reports 17: 1326‑1332, 2018; DOI: 10...
August 9, 2018: Molecular Medicine Reports
Qingsong Zuo, Jie Wang, Chao Chen, Yong Zhang, Dian-Xu Feng, Ronghua Zhao, Teng Chen
Achaete‑scute homolog 2 (ASCL2), a basic helix‑loop‑helix transcription factor, serves an essential role in the maintenance of adult intestinal stem cells and the growth of gastric cancer (GC). However, the function of ASCL2 in the metastasis of GC is poorly understood. The present study aimed to evaluate the effect of ASCL2 expression on gastric tumor metastasis. ASCL2 protein expression was detected in 32 cases of gastric metastasis and its relevant primary tumors using western blotting and immunohistochemistry...
August 8, 2018: Molecular Medicine Reports
Yan Feng, Xing Guo, Xinping Huang, Manya Wu, Xin Li, Shushu Wu, Xiaoling Luo
Cancer stem cells (CSCs) have been reported to be associated with the recurrence and drug resistance of liver cancer. In the present study, stem cell‑like HepG2 cell spheres were enriched using stem cell conditioned culture medium. As expected, stem‑like HepG2 cell spheres exhibited increased resistance to sorafenib. Metformin, a common drug used to treat type 2 diabetes mellitus, reduced the diameters and numbers of stem‑like HepG2 spheres, and increased their sensitivity to sorafenib. Western blotting confirmed that low doses of metformin reversed the epithelial‑mesenchymal transformation (EMT) process of HepG2 spheres...
August 3, 2018: Molecular Medicine Reports
Jun Lei, Yong Chai, Juhua Xiao, Huakun Hu, Zhiqiang Liu, Yu Xiao, Lijun Yi, Jinshi Huang, Tianxin Xiang, Shouhua Zhang
Biliary atresia (BA) is a rare and severe disease that affects infants where a fibroinflammatory process destroys the bile ducts, leading to fibrosis and biliary cirrhosis, and mortality if untreated. Bone marrow‑derived mesenchymal stem cells (BMMSCs) have been considered as a promising therapy in fibrotic diseases. The aim of the present was to investigate the anti‑fibrotic roles of BMMSC transplantation in a BA mouse model. Mouse BA models were established by Rhesus rotavirus administration to neonatal mice...
August 3, 2018: Molecular Medicine Reports
Weiheng Wang, Xiaodong Huang, Wenbo Lin, Yuanyuan Qiu, Yunfei He, Jiangming Yu, Yanhai Xi, Xiaojian Ye
The unique hypoxic inflammatory microenvironment observed in the spinal cord following spinal cord injury (SCI) limits the survival and efficacy of transplanted bone mesenchymal stem cells (BMSCs). The aim of the present study was to determine whether hypoxic preconditioning (HP) increased the therapeutic effects of BMSC on SCI. BMSCs were pretreated with cobalt chloride (CoCl2) in vitro, and the proliferative apoptotic and migratory abilities of these hypoxic BMSCs (H‑BMSCs) were assessed. BMSCs and H‑BMSCs derived from green fluorescent protein (GFP) rats were transplanted into SCI rats in vivo...
August 7, 2018: International Journal of Molecular Medicine
Shamira Sridharan, Yanfen Li, Louis Foucard, Hassaan Majeed, Basanta Bhaduri, Alex Levine, Kristopher Kilian, Gabriel Popescu
Characterizing the effects of force fields generated by cells on proliferation, migration, and differentiation processes is challenging due to limited availability of non-destructive imaging modalities. Here, we integrate a new real-time traction stress imaging modality, Hilbert phase dynamometry (HPD), with spatial light interference microscopy (SLIM) for simultaneous monitoring of cell growth during differentiation processes. HPD uses holographic principles to extract displacement fields from chemically patterned fluorescent grid on deformable substrates...
August 14, 2018: Journal of Biophotonics
Bettina Hafen, Susanne Wiesner, Katrin Schlegelmilch, Alexander Keller, Lothar Seefried, Regina Ebert, Heike Walles, Franz Jakob, Norbert Schütze
Multipotent adult stem cells/precursor cells, especially of the mesenchymal and endothelial lineage, may have great potential for bone tissue engineering. Although their potential is highly recognized, not much is known about the underlying molecular mechanisms that initiate the regeneration process, connect osteogenesis, and angiogenesis and, finally, orchestrate renewal of bone tissue. Our study addressed these questions by generating two in vitro cell culture models to examine the changes in the global gene expression patterns of endothelial precursor cells and mesenchymal stem cells after 24 hours of either humoral (conditioned medium) or direct cell-cell interaction (co-culture)...
August 13, 2018: Journal of Cellular Biochemistry
Lin-Liang Wang, Xue-Feng Yin, Xiu-Cheng Chu, Yong-Bing Zhang, Xiao-Nan Gong
As a common cause of shoulder pain and disability, rotator cuff injury (RCI) represents a debilitating condition affecting an individual's quality of life. Although surgical repair has been shown to be somewhat effective, many patients may still suffer from reduced shoulder function. The aim of the current study was to identify a more effective mode of RCI treatment by investigating the effect of platelet-derived growth factor subunit B (PDGF-B) on tendon-bone healing after RCI repair by modifying bone marrow-derived mesenchymal stem cells (BMSCs)...
August 13, 2018: Journal of Cellular Biochemistry
Halimeh Hassanzadeh, Maryam M Matin, Hojjat Naderi-Meshkin, Hamid Reza Bidkhori, Mahdi Mirahmadi, Mahmood Raeesolmohaddeseen, Nasser Sanjar-Moussavi, Ahmad Reza Bahrami
Recent advances in wound healing have made cell therapy a potential approach for the treatment of various types of skin defects such as trauma, burns, scars and diabetic leg ulcers. Cultured keratinocytes have been applied to burn patients since 1981. Patients with acute and chronic wounds can be treated with autologous/allograft cultured keratinocytes. There are various methods for cultivation of epidermal keratinocytes used in cell therapy. One of the important properties of an efficient cell therapy is the preservation of epidermal stem cells...
August 13, 2018: Cell and Tissue Banking
Carola Millan, Juan F Vivanco, Isabel M Benjumeda-Wijnhoven, Suncica Bjelica, Juan F Santibanez
In orthopedic medicine, a feasible reconstruction of bone structures remains one of the main challenges both for healthcare and for improvement of patients' quality of life. There is a growing interest in mesenchymal stem cells (MSCs) medical application, due to their multilineage differentiation potential, and tissue engineering integration to improve bone repair and regeneration. In this review we will describe the main characteristics of MSCs, such as osteogenesis, immunomodulation and antibacterial properties, key parameters to consider during bone repair strategies...
August 14, 2018: Advances in Experimental Medicine and Biology
Laura Otero-Ortega, Fernando Laso-García, MariCarmen Gómez-de Frutos, Blanca Fuentes, Luke Diekhorst, Exuperio Díez-Tejedor, María Gutiérrez-Fernández
Approximately, 16 million strokes occur worldwide each year, causing 6 million deaths and considerable disability, implying an enormous social, individual health, and economic burden. Due to this high incidence, strategies to promote stroke recovery are urgently needed. Research into new therapeutic approaches for stroke has determined that intravenous administration of mesenchymal stem cells (MSCs) is a good strategy to improve recovery by amplifying mechanisms implicated in brain plasticity. Recent studies have demonstrated the efficacy of MSCs in stroke, with no need for them to reach the area of brain injury...
August 13, 2018: Translational Stroke Research
Min-Kyung Shin, Bongkun Choi, Eun-Young Kim, Ji-Eun Park, Eui Seung Hwang, Hyang Ju Lee, Min Kyung Kim, Ji-Eun Kim, Seong Who Kim, Eun-Ju Chang
Obesity is accompanied by chronic systemic inflammation characterized by macrophage infiltration of obese tissues, an elevated plasma level of inflammatory substances, and excessive accumulation of lipids. The pro-inflammatory factor pentraxin 3 (PTX3) is also elevated in obese tissues, suggesting its potential role in adipogenesis. We found by analyzing murine preadipocyte 3T3-L1 cells, and human adipocytes derived from mesenchymal stem cells, which locally elevated PTX3 in obese adipose tissue augments adipocyte differentiation and subsequent lipid accumulation...
2018: Frontiers in Immunology
Isaac Peña-Villalobos, Ignacio Casanova-Maldonado, Pablo Lois, Catalina Prieto, Carolina Pizarro, José Lattus, Germán Osorio, Verónica Palma
Hyperbaric oxygen therapy (HBOT) is effective for the medical treatment of diverse diseases, infections, and tissue injury. In fact, in recent years there is growing evidence on the beneficial effect of HBOT on non-healing ischemic wounds. However, there is still yet discussion on how this treatment could benefit from combination with regenerative medicine strategies. Here we analyzed the effects of HBOT on three specific aspects of tissue growth, maintenance, and regeneration: (i) modulation of adult rodent ( Mus musculus ) intestinal stem cell turnover rates; (ii) angiogenesis dynamics during the development of the chorio-allantoic membrane (CAM) in Gallus gallus embryos; (iii) and wound-healing in a spontaneous type II diabetic mouse model with a low capacity to regenerate skin...
2018: Frontiers in Physiology
Yi-Heng Lin, Ya-Hsin Chen, Heng-Yu Chang, Heng-Kien Au, Chii-Ruey Tzeng, Yen-Hua Huang
Endometriosis is an estrogen-dependent inflammatory disease that affects up to 10% of women of reproductive age and accounts for up to 50% of female infertility cases. It has been highly associated with poorer outcomes of assisted reproductive technology (ART), including decreased oocyte retrieval, lower implantation, and pregnancy rates. A better understanding of the pathogenesis of endometriosis-associated infertility is crucial for improving infertility treatment outcomes. Current theories regarding how endometriosis reduces fertility include anatomical distortion, ovulatory dysfunction, and niche inflammation-associated peritoneal or implantation defects...
August 13, 2018: International Journal of Molecular Sciences
Jaleh Varshosaz, Hajar Asefi, Batool Hashemi-Beni, Solmaz Ghaffari, Ali Davoudi
Chondroitin sulphate is a sulphated glycosaminoglycan biopolymer composed over 100 individual sugars. Chondroitin sulphate nanoparticles (NPs) loaded with catechin were prepared by an ionic gelation method using AlCl3 and optimised for polymer and cross-linking agent concentration, curing time and stirring speed. Zeta potential, particle size, loading efficiency, and release efficiency over 24 h (RE24 %) were evaluated. The surface morphology of NPs was investigated by scanning electron microscopy and their thermal behaviour by differential scanning calorimetric...
September 2018: IET Nanobiotechnology
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
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