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mesenchymal stem, endothelial cells,

Naglaa K Idriss, Hayam G Sayyed, Amany Osama, Dina Sabry
BACKGROUND/AIMS: The most appropriate route for bone marrow-derived mesenchymal stem cell (BM-MSC) transplantation in the management of liver fibrosis remains controversial. This study investigated the therapeutic efficacy of intravenous and intrasplenic BM-MSC transplantation on carbon tetrachloride (CCl4)-induced rat liver fibrosis. METHODS: Fifty rats were divided into 5 groups (n = 10 rats per group): healthy control group, CCl4 group, CCl4/ recovery group, CCl4/BM-MSC intravenous group, and CCl4/BM-MSC intrasplenic group...
August 16, 2018: Cellular Physiology and Biochemistry
Zheng Chen, Qianqian Chen, Haitao Du, Lijuan Xu, Jun Wan
The present study intended to observe the homing capability and therapeutic effect of CXC chemokine receptor 4 (CXCR-4) gene overexpressed bone marrow mesenchymal stem cells (BMSCs) on colitis, and to study the possible mechanisms involved. BMSCs were derived from male BALB/c mice and CXCR-4 gene was transfected into BMSCs by the utilization of the lentiviral vector. The expression of CXCR-4 gene was analyzed and the biological characteristics, and vitality of BMSCs and CXCR-4 gene overexpressed BMSCs (CXCR-BMSCs) were detected...
August 2018: Experimental and Therapeutic Medicine
Tatiana Lopatina, Cristina Grange, Valentina Fonsato, Marta Tapparo, Alessia Brossa, Sofia Fallo, Adriana Pitino, Maria Beatriz Herrera-Sanchez, Sharad Kholia, Giovanni Camussi, Benedetta Bussolati
Human liver stem-like cells (HLSC) and derived extracellular vesicles (EVs) were previously shown to exhibit anti-tumor activity. In the present study, we investigated whether HLSC-derived EVs (HLSC-EVs) were able to inhibit tumor angiogenesis in vitro and in vivo, in comparison with EVs derived from mesenchymal stem cells (MSC-EVs). The results obtained indicated that HLSC-EVs, but not MSC-EVs, inhibited the angiogenic properties of tumor-derived endothelial cells (TEC) both in vitro and in vivo in a model of subcutaneous implantation in Matrigel...
August 15, 2018: International Journal of Cancer. Journal International du Cancer
Kyohei Kin, Takao Yasuhara, Masahiro Kameda, Yousuke Tomita, Michiari Umakoshi, Ken Kuwahara, Ittetsu Kin, Naoya Kidani, Jun Morimoto, Mihoko Okazaki, Tatsuya Sasaki, Naoki Tajiri, Cesario V Borlongan, Isao Date
Despite the advances in pharmacological therapies, only the half of depressed patients respond to currently available treatment. Thus, the need for further investigation and development of effective therapies, especially those designed for treatment-resistant depression, has been sorely needed. Although antidepressant effects of mesenchymal stem cells (MSCs) have been reported, the potential benefit of this cell therapy on treatment-resistant depression is unknown. Cell encapsulation may enhance the survival rate of grafted cells, but the therapeutic effects and mechanisms mediating encapsulation of MSCs remain unexplored...
August 14, 2018: Molecular Psychiatry
Bettina Hafen, Susanne Wiesner, Katrin Schlegelmilch, Alexander Keller, Lothar Seefried, Regina Ebert, Heike Walles, Franz Jakob, Norbert Schütze
Multipotent adult stem cells/precursor cells, especially of the mesenchymal and endothelial lineage, may have great potential for bone tissue engineering. Although their potential is highly recognized, not much is known about the underlying molecular mechanisms that initiate the regeneration process, connect osteogenesis, and angiogenesis and, finally, orchestrate renewal of bone tissue. Our study addressed these questions by generating two in vitro cell culture models to examine the changes in the global gene expression patterns of endothelial precursor cells and mesenchymal stem cells after 24 hours of either humoral (conditioned medium) or direct cell-cell interaction (co-culture)...
August 13, 2018: Journal of Cellular Biochemistry
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
Chunli Zhang, Yifei Du, Hua Yuan, Fei Jiang, Ming Shen, Yuli Wang, Ruixia Wang
Osteoporosis and vascular lesions induced by glucolipotoxicity are common complications of diabetes mellitus (DM). In order to deal with these complications, we designed a new therapeutic strategy, i.e. coculture system containing human amnion-derived mesenchymal stem cells (HAMSCs) and human bone marrow mesenchymal stem cells (HBMSCs). Two in vitro coculture models, transwell and mixed cocultures, were proposed for 7 days with variable HAMSCs: HBMSCs ratios. Then, supernatant from each coculture was used to reverse the deficiency of HBMSCs and human umbilical vein endothelial cells (HUVECs) impaired by high glucose and palmitic acid (GP)...
September 2018: Biochimie
Michel Aurrand-Lions, Stéphane J C Mancini
After birth, the development of hematopoietic cells occurs in the bone marrow. Hematopoietic differentiation is finely tuned by cell-intrinsic mechanisms and lineage-specific transcription factors. However, it is now clear that the bone marrow microenvironment plays an essential role in the maintenance of hematopoietic stem cells (HSC) and their differentiation into more mature lineages. Mesenchymal and endothelial cells contribute to a protective microenvironment called hematopoietic niches that secrete specific factors and establish a direct contact with developing hematopoietic cells...
August 10, 2018: International Journal of Molecular Sciences
Guido Carpino, Vincenzo Cardinale, Trine Folseraas, Diletta Overi, Krzysztof Grzyb, Daniele Costantini, Pasquale Bartolomeo Berloco, Sabina Di Matteo, Tom Hemming Karlsen, Domenico Alvaro, Eugenio Gaudio
Primary sclerosing cholangitis (PSC) is a chronic inflammatory cholangiopathy frequently complicated by cholangiocarcinoma (CCA). Massive proliferation of biliary tree stem/progenitor cells (BTSCs), expansion of peribiliary glands (PBGs), and dysplasia were observed in PSC. The aims of the present study were to evaluate the involvement of PBGs and BTSCs in CCA which emerged in PSC patients. Specimens from normal liver (N=5), PSC (N=20), and PSC-associated CCA (N=20) were included. Samples were processed for histology, immunohistochemistry and immunofluorescence...
August 13, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Ming-Song Lee, Jesse Wang, Huihua Yuan, Hongli Jiao, Tsung-Lin Tsai, Matthew W Squire, Wan-Ju Li
Blood vessels composed of endothelial cells (ECs) contact with mesenchymal stem cells (MSCs) in different tissues, suggesting possible interaction between these 2 types of cells. We hypothesized that endothelin-1 (ET1), a secreted paracrine factor of ECs, can differentially direct the lineages of adipose-derived stem cells (ASCs) and bone marrow-derived MSCs (BMSCs). Predifferentiated ASCs and BMSCs were treated with ET1 for 2 cell passages and then induced for multilineage differentiation. Our results showed that adipogenesis of ET1-pretreated ASCs and osteogenesis of ET1-pretreated BMSCs were increased compared to those of control cells...
August 10, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Yong-Chao Jiang, Xiao-Feng Wang, Yi-Yang Xu, Yu-Hui Qiao, Xin Guo, Dong-Fang Wang, Qian Li, Lih-Sheng Turng
During the regeneration of tissues and organs, growth factors (GFs) play a vital role by affecting cell behavior. However, because of the low half-life time and quick degradation of GFs, their stimulations on cells are relatively short and discontinuous. In this study, a releasing scaffold platform, consisting of polycaprolactone (PCL) nanofibers and vascular endothelial growth factor (VEGF)-encapsulated gelatin particles, was developed to extend the influence of GFs on mesenchymal stem cells (MSCs) and endothelial cells (ECs)...
August 10, 2018: Biomacromolecules
Jingrui Chen, Jing Wei, Yuting Huang, Yuling Ma, Jingyu Ni, Min Li, Yan Zhu, Xiumei Gao, Guanwei Fan
Stem cell-based therapies have the potential to dramatically transform the treatment and prognosis of myocardial infarction (MI), and mesenchymal stem cells (MSCs) have been suggested as a promising cell population to ameliorate the heart remodeling in post-MI. However, poor implantation and survival in ischemic myocardium restrict its efficacy and application. In this study, we sought to use the unique mode of action of Chinese medicine to improve this situation. Surrounding the myocardial infarct area, we performed a multi-point MSC transplantation and administered in conjunction with Danhong injection, which is mainly used for the treatment of MI...
2018: Frontiers in Physiology
Rana Keyhanmanesh, Reza Rahbarghazi, Mahdi Ahmadi
Achieving the optimal clinical outcome of mesenchymal stem cells (MSCs) is particularly dependent on fundamental understanding of therapeutic mechanisms. The current study was focused on the possible mechanisms by which rat bone marrow-derived mesenchymal stem cells (rBMMSCs) and/or conditioned media (CM) display broad immunomodulatory properties for ameliorating of asthma-related pathological changes. Male rats were divided equally into four experimental groups (n = 6): healthy rats received 50 μl PBS intravenously (group C), sensitized rats received 50 μl PBS intravenously (group OVA), sensitized rats received 50 μl CM intravenously (group OVA + CM), and sensitized rats received 50 μl PBS intravenously containing 2 × 106 rBMMSCs (group OVA + MSCs)...
August 7, 2018: Inflammation
Wuzheng Zhu, Yujia Yuan, Guangneng Liao, Lan Li, Jingping Liu, Younan Chen, Jie Zhang, Jingqiu Cheng, Yanrong Lu
Mitochondrial dysfunction and excessive mitochondrial reactive oxygen species (ROS) are fundamental contributors to endothelial injury in diabetic states. Mesenchymal stem cells (MSCs) have exhibited an extraordinary cytoprotective effect that extends to the modulation of mitochondrial homeostasis. However, the underlying mechanisms have not been clearly defined. Emerging evidence has suggested that mitophagy could counteract mitochondrial-derived oxidative stress through the selective elimination of impaired or dysfunctional mitochondria...
August 6, 2018: Cell Death & Disease
Jiahui Du, Peng Xie, Shuxian Lin, Yuqiong Wu, Deliang Zeng, Yulin Li, Xinquan Jiang
The survival of engineered constructs in large bone defects depends on the early formation of new blood vessel networks to guarantee the effective exchange of oxygen, nutrients and metabolic wastes. Adipose-derived mesenchymal stem cells (ADSCs), which are considered excellent candidate cells for both bone and blood vessel regeneration, are the preferred choice for the restoration of massive bone defects. In the present study, we proposed to induce ADSCs differently in vitro into osteogenic cells and endothelial cells...
August 6, 2018: ACS Applied Materials & Interfaces
Tanongsak Laowanitwattana, Sirinda Aungsuchawan, Suteera Narakornsak, Runchana Markmee, Waleephan Tancharoen, Junjira Keawdee, Nonglak Boonma, Witoon Tasuya, Lamaiporn Peerapapong, Nathaporn Pangjaidee, Peeraphan Pothacharoen
Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest...
August 2, 2018: Acta Histochemica
Ju Hun Yeon, Hyo Eun Jeong, Hyemin Seo, Siwoo Cho, Kimin Kim, Dokyun Na, Seok Chung, Jaesung Park, Nakwon Choi, Ji Yoon Kang
Cancer-associated fibroblasts (CAFs) play a pivotal role in tumor growth, but very little has been known about its characteristics and origin. Recently, cancer-derived exosome has been suggested to transdifferentiate CAFs, by a new mechanism of endothelial to mesenchymal transition (EndMT), initiating angiogenic processes and triggering metastatic evolution. However, an enabling tool in vitro is yet to be developed to investigate complicated procedures of the EndMT and the transdifferentiation under reconstituted tumor microenvironment...
August 2018: Acta Biomaterialia
Shirley Man, Gonzalo Sanchez Duffhues, Peter Ten Dijke, David Baker
Endothelial cells (ECs) have been found to be capable of acquiring a mesenchymal phenotype through a process known as endothelial-to-mesenchymal transition (EndMT). First seen in the developing embryo, EndMT can be triggered postnatally under certain pathological conditions. During this process, ECs dedifferentiate into mesenchymal stem-like cells (MSCs) and subsequently give rise to cell types belonging to the mesoderm lineage. As EndMT contributes to a multitude of diseases, pharmacological modulation of the signaling pathways underlying EndMT may prove to be effective as a therapeutic treatment...
August 3, 2018: Angiogenesis
Ziba Roveimiab, Francis Lin, Judy Anderson
Cell migration is an essential process in which cells move from one location to another with different modes, including mesenchymal, amoeboid or collective movements. Migration occurs during development and in the maintenance of multicellular organisms for purposes of wound healing, tissue regeneration, and immune and pathophysiological responses. Cells in all three types of muscle: cardiac, smooth, and skeletal, are subject to and undergo migration, both general and adapted for tissue-specific needs. Cardiac cell migration is mediated by vascular endothelial growth factor (VEGF) through expression of VEGF receptors; it is not clear how cardiac cells migrate into a region of damage after infarction...
August 3, 2018: Tissue Engineering. Part B, Reviews
Min Bi, Jiawei Wang, Yidan Zhang, Longzhu Li, Linhui Wang, Ran Yao, Shijie Duan, Suijun Tong, Jianpeng Li
Bone marrow mesenchymal stem cells (BMSCs) are used as a great promising choice for the treatment of cerebral ischemia. Herein, we discuss the neuroprotective effects of the combination of BMSCs transplantation and mild hypothermia (MH) in an ischemia-reperfusion rat model. First, BMSCs were isolated using density gradient centrifugation and the adherent screening method, followed by culture, identification and labeling with DAPI. Second, adult male SD rats were divided into 5 groups: sham group (surgery without blockage of middle cerebral artery), model group (middle cerebral artery occlusion (MCAO) was established 2h prior to reperfusion), BMSCs group (injection of BMSCs via the lateral ventricle 24h after MCAO), MH group (mild hypothermia for 3h immediately after MCAO) and combination therapy group (combination of BMSCs and MH)...
2018: PloS One
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