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mesenchymal stem, endothelial cells,

Takashi Kido, Shigeru Miyagawa, Takasumi Goto, Katsuto Tamai, Takayoshi Ueno, Koichi Toda, Toru Kuratani, Yoshiki Sawa
OBJECTIVES: We hypothesized that systemic administration of high-mobility group box 1 fragment attenuates the progression of myocardial fibrosis and cardiac dysfunction in a hamster model of dilated cardiomyopathy by recruiting bone marrow mesenchymal stem cells thus causing enhancement of a self-regeneration system. METHODS: Twenty-week-old J2N-k hamsters, which are δ-sarcoglycan-deficient, were treated with systemic injection of high-mobility group box 1 fragment (HMGB1, n = 15) or phosphate buffered saline (control, n = 11)...
2018: PloS One
Yingting Yu, Qiaojun Situ, Wangyue Jia, Junxiang Li, Qiong Wu, Jinzhi Lei
Coculture of mesenchymal stem cells (MSCs) and vascular endothelial cells (ECs) in vitro leads to the formation of a capillary-like reticular structure by ECs, which has great potential as a better substitute for artificial blood vessels in terms of stability and functionality. To investigate the mechanisms of the early neovascularization induced by MSCs, we analyzed the kinematic features of the motion of ECs and concluded that the dynamic interaction between cells and the extracellular matrix would reveal the capillary-like structure formation...
December 5, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Joanna Maria Sadowska, Jordi Guillem-Marti, Maria-Pau Ginebra
Calcium phosphate (CaP) substrates are successfully used as bone grafts due to their osteogenic properties. However, the influence of the physicochemical features of CaPs in angiogenesis is frequently neglected despite it being a crucial process for bone regeneration. The present work focuses on analyzing the effects of textural parameters of biomimetic calcium deficient hydroxyapatite (CDHA) and sintered beta-tricalcium phosphate (β-TCP), such as specific surface area, surface roughness, and microstructure, on the behavior of rat endothelial progenitor cells (rEPCs) and their crosstalk with rat mesenchymal stem cells (rMSCs)...
December 5, 2018: Advanced Healthcare Materials
Kaya Xu, Jea-Young Lee, Yuji Kaneko, Julian P Tuazon, Fernando Vale, Harry van Loveren, Cesario V Borlongan
Despite mounting evidence of a massive peripheral inflammatory response accompanying stroke, the ability of intracerebrally transplanted cells to migrate to the periphery and sequester systemic inflammation remains unexamined. Here, we tested the hypothesis that human bone marrow mesenchymal stromal cells intracerebrally transplanted in the brain of adult rats subjected to experimental stroke can migrate to the spleen, a vital organ that confers peripheral inflammation after stroke. Adult Sprague-Dawley rats received sham or experimental stroke via the one-hour middle cerebral artery occlusion model...
December 4, 2018: Haematologica
Wei Hu, Jian Jiang, Feng Yang, Jun Liu
INTRODUCTION: The impact of bone marrow-derived mesenchymal stem cells (BM-MSCs) on neovascularisation in rats with craniocerebral injury (CI) was investigated. MATERIAL AND METHODS: The rate CI model was established by the free-fall method, and then rats were randomly divided into a transplanted group (EG) and a control group (CG). Rats in the transplanted group were injected with BM-MSCs in the lateral ventricle, while those in the control group were injected with normal saline...
2018: Folia Neuropathologica
Anatoly I Soloviev, Igor V Kizub
Ionizing radiation (IR) leads to a variety of the cardiovascular diseases, including the arterial hypertension. A number of studies have demonstrated that blood vessels represent important target for IR, and the endothelium is one of the most vulnerable components of the vascular wall. IR causes an inhibition of nitric oxide (NO)-mediated endothelium-dependent vasodilatation and generation of reactive oxygen (ROS) and nitrogen (RNS) species trigger this process. Inhibition of NO-mediated vasodilatation could be due to endothelial NO synthase (eNOS) down-regulation, inactivation of endothelium-derived NO, and abnormalities in diffusion of NO from the endothelial cells (ECs) leading to a decrease in NO bioavailability...
November 30, 2018: Biochemical Pharmacology
Jenna N Harvestine, Hakan Orbay, Jonathan Y Chen, David E Sahar, J Kent Leach
Lipoaspirates contain a readily accessible heterogeneous cell source for use in bone regeneration collectively referred to as the stromal vascular fraction (SVF). However, the osteogenic potential of SVF is inferior to other progenitor cell populations, thereby requiring alternative strategies to potentiate its effective use in cell-based therapies of bone repair. Cell-secreted extracellular matrix (ECM) is a promising substrate to guide cell phenotype or for use in biomaterial design, yet the instructional capacity of ECMs produced by various cell types is unknown...
June 28, 2018: Journal of Materials Chemistry. B, Materials for Biology and Medicine
Seo-Hyun Choi, A-Ram Kim, Jae-Kyung Nam, Jin-Mo Kim, Jee-Youn Kim, Haeng Ran Seo, Hae-June Lee, Jaeho Cho, Yoon-Jin Lee
It remains controversial whether targeting tumour vasculature can improve radiotherapeutic efficacy. We report that radiation-induced endothelial-to-mesenchymal transition (EndMT) leads to tumour vasculature with abnormal SMA+ NG2+ pericyte recruitment during tumour regrowth after radiotherapy. Trp53 (but not Tgfbr2) deletion in endothelial cells (ECs) inhibited radiation-induced EndMT, reducing tumour regrowth and metastases with a high CD44v6+ cancer-stem-cell (CSC) content after radiotherapy. Osteopontin, an EndMT-related angiocrine factor suppressed by EC-Trp53 deletion, stimulated proliferation in dormant CD44v6+ cells in severely hypoxic regions after radiation...
November 30, 2018: Nature Communications
Jun-Qiu Chen, Liang-Hu Huang
Mesenchymal stem cells (MSC) have the potential of multi-directional differentiation, and can recruit endothelial cells, promote their proliferation, migration and angiogenesis, improve blood perfusion and oxygen suppliment, and repair damaged tissue. Exosome secreted by MSC contain mother cell-specific proteins, lipids and nucleic acids, and acts as signaling molecule, playing an important role in cell communication, thereby altering target cell function. In this review, the biological characteristics of MSC and its exosome, the mechanism of promoting vascular regeneration in patients with ischemic diseases, and the mechanism of hypoxia-inducible factor-1α(HIF-1α) in the vascular ischemia of ischemic diseases are all summarized briefly...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Shulin Li, Yuan Zhao, Zhuojun Wang, Jia Wang, Caixia Liu, Dong Sun
Amniotic fluid-derived stem cells (AFSCs), which exhibit both embryonic and mesenchymal stem cell characteristics, have been shown to mitigate the degree of renal interstitial fibrosis. The aim of the present study was to determine whether transplantation of glial cell line-derived neurotrophic factor (GDNF)-modified AFSCs is more useful than transplantation of unmodified AFSCs for the treatment of renal interstitial fibrosis. Mice were randomly assigned to a sham-operation group (sham), a unilateral ureteral obstruction (UUO)-saline solution group (UUO), an AFSC transplantation group (AFSC) and a GDNF-modified AFSC transplantation group (GDNF-AFSC) and sacrificed at days 3 and 7 post-surgery (six in each group)...
November 30, 2018: Cell Transplantation
Yingxin Zhang, Jie Ding, Cong Xu, Hongli Yang, Peng Xia, Shengjun Ma, Haiying Chen
Background and Objectives: Previous studies have shown that integrins alpha5beta1 (ITGA5B1) gene-modified rat bone marrow mesenchymal stem cells (rBMSCs) could prevent cell anoikis and increase the nitric oxide (NO) production. Here we examined the capability of rBMSCs/ITGA5B1 on the phenotype modulation of Human Pulmonary Artery Smooth Muscle Cell (HPASMC) in vitro. Methods and Results: The synthetic (dedifferentiated) phenotype of HPASMC was induced by monocrotaline (MCT, 1 μ M) for 24 h and then co-cultured with rBMSCs/ITGA5B1 in a transwell culture system...
November 30, 2018: International Journal of Stem Cells
Marta Pokrywczynska, Arkadiusz Jundzill, Marta Rasmus, Jan Adamowicz, Daria Balcerczyk, Monika Buhl, Karolina Warda, Lukasz Buchholz, Maciej Gagat, Dariusz Grzanka, Tomasz Drewa
BACKGROUND: The tissue engineering of urinary bladder advances rapidly reflecting clinical need for a new kind of therapeutic solution for patients requiring urinary bladder replacement. Majority of the bladder augmentation studies have been performed in small rodent or rabbit models. Insufficient number of studies examining regenerative capacity of tissue-engineered graft in urinary bladder augmentation in a large animal model does not allow for successful translation of this technology to the clinical setting...
November 28, 2018: Stem Cell Research & Therapy
Vladimir Mulens-Arias, Alice Balfourier, Alba Nicolás-Boluda, Florent Carn, Florence Gazeau
Gold nanoparticles (AuNP) have been thoroughly studied as multifunctional theranosis agents for cell imaging and cancer therapy as well as sensors due to their tunable physical and chemical properties. Although AuNP have proved to be safe in a wide concentration range, yet other important biological effects can arise in the sublethal window of treatment. This is especially pivotal to understand how AuNP can affect cell biology when labeling steps are needed for cell tracking in vivo, as nanoparticle loading can affect cell migratory/invasion ability, a function mediated by filamentous actin-rich nanometric structures collectively called adhesomes...
November 28, 2018: Biomaterials Science
Hiroshi Yabuki, Tatsuaki Watanabe, Hisashi Oishi, Masato Katahira, Masahiko Kanehira, Yoshinori Okada
Ischemia-reperfusion injury (IRI) is one of the main causes of primary graft dysfunction that accounts for 25% of mortality after lung transplantation. Disruption of blood supply and subsequent reperfusion result in organ damage with activating innate and adaptive immune response, leading to inflammatory insults. The IRI after lung transplantation is primarily manifested by permeability pulmonary edema on the basis of pulmonary vascular endothelial cell injury as seen in acute respiratory distress syndrome (ARDS)...
2018: Advances in Experimental Medicine and Biology
Katsuhiro Hosoyama, Yoshikatsu Saiki
The aorta is a well-organized, multilayered structure comprising several cell types, namely, endothelial cells (ECs), vascular smooth muscle cells (VSMCs), and fibroblasts, as well as an extracellular matrix (ECM), which includes elastic and collagen fibers. Aortic aneurysms (AAs) are defined as progressive enlargements of the aorta that carries an incremental risk of rupture as the diameter increases over time. The destruction of the aortic wall tissue is triggered by atherosclerosis, inflammation, and oxidative stress, leading to the activation of matrix metalloproteinases (MMPs), and inflammatory cytokines and chemokines, resulting in the loss of the structural back bone of VSMCs, ECM, and ECs...
2018: Advances in Experimental Medicine and Biology
Anne Wilson, He Fu, Mariano Schiffrin, Carine Winkler, Meriem Koufany, Jean-Yves Jouzeau, Nicolas Bonnet, Federica Gilardi, François Renevey, Sanjiv A Luther, David Moulin, Béatrice Desvergne
Adult hematopoiesis takes place in the perivascular zone of the bone cavity, where endothelial cells, mesenchymal stromal/stem cells and their derivatives such as osteoblasts are key components of bone marrow (BM) niches. Defining the contribution of BM adipocytes to the hematopoietic stem cell niche remains controversial. While an excess of medullar adiposity is generally considered deleterious for hematopoiesis, an active role for adipocytes in shaping the niche has also been proposed. We thus investigated the consequences of total adipocyte deletion, including in the BM niche, on adult hematopoiesis using mice carrying a constitutive deletion of the gene coding for the nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ)...
2018: Frontiers in Immunology
S F Yuan, Z Y Liu, Y J M G L Ayi, R J Zheng, Y X Zhang
Objective: To investigate the curative effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of stromal cell-derived growth factor (SDF-1 α) and vascular endothelial growth factor (VEGF) in rats with acute hepatic failure, and to compare the effects of two transplantation pathways. Methods: Eighty-four rats with acute liver failure (ALF) induced by D-galactosamine combined with lipopolysaccharide were randomly divided into control group, tail vein and portal vein transplantation group...
October 20, 2018: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
Xiaoya Wang, Long Gao, Yan Han, Min Xing, Cancan Zhao, Jinliang Peng, Jiang Chang
The enhancement of adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and sufficient vascularization remain great challenges for the successful reconstruction of engineered adipose tissue. Here, the bioactive effects of silicon (Si) ions on adipogenic differentiation of human BMSCs (HBMSCs) and the stimulation of vascularization during adipose tissue regeneration are reported. The results show that Si ions can enhance adipogenic differentiation of HBMSCs through the stimulation of the expression of adipogenic differentiation switches such as peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α...
November 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Kayoko Kitajima, Ridhima Das, Xiao Liang, Evelyn Neppelberg, Anne Christine Johannessen, Daniela Elena Costea, Masaru Igarashi
The epithelial rests of Malassez (ERMs) might represent a valuable source of oral epithelial cells with stem cell properties. The purpose of this study was to isolate and characterize cells derived from human ERM, and compare them with cells derived from matched normal oral mucosa (NOM). Matched tissue specimens of the periodontal ligament of extracted tooth and NOM were collected. Cells were isolated in culture, then characterized by immunohistochemistry and flow cytometry for expression of pancytokeratin, ESA, PDGFRB, CD31 and CD44...
November 26, 2018: Odontology
Seung Hwan Jeon, Guan Qun Zhu, Woong Jin Bae, Sae Woong Choi, Hyun Cheol Jeong, Hyuk Jin Cho, U-Syn Ha, Sung-Hoo Hong, Ji Youl Lee, Eun Bi Kwon, Hyo-Jin Kim, Soon Min Lee, Hey-Yon Kim, Sae Woong Kim
Effective therapies for erectile dysfunction (ED) associated with diabetes mellitus (DM) are needed. In this study, the effects of stromal cell-derived factor-1 (SDF-1)-expressing engineered mesenchymal stem cells (SDF-1 eMSCs) and the relevant mechanisms in the corpus cavernosum of a streptozotocin (STZ)-induced DM ED rat model were evaluated. In a randomized controlled trial, Sprague⁻Dawley (SD) rats ( n = 48) were divided into four groups ( n = 12/group): Normal (control), DM ED (diabetes induced by STZ), DM ED + BM-MSC (treated with bone marrow [BM]-derived MSCs), and DM ED + SDF-1 eMSC (treated with SDF-1-expressing BM-MSCs)...
November 23, 2018: International Journal of Molecular Sciences
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