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https://www.readbyqxmd.com/read/30333762/critical-flicker-fusion-frequency-a-marker-of-cerebral-arousal-during-modified-gravitational-conditions-related-to-parabolic-flights
#1
Costantino Balestra, Marie-Laure Machado, Sigrid Theunissen, Ambre Balestra, Danilo Cialoni, Christian Clot, Stépane Besnard, Laura Kammacher, Julie Delzenne, Peter Germonpré, Pierre Lafère
In situ evaluation of human brain performance and arousal remains challenging during operational circumstances, hence the need for a rapid, reliable and reproducible tool. Here we hypothesized that the Critical flicker fusion frequency (CFFF) reflecting/requiring visual integration, visuo-motor skills and decision-taking process might be a powerful, fast and simple tool in modified gravity environments. Therefore 11 male healthy volunteers were assessed for higher cognitive functions with CFFF during parabolic flights...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/27362880/determining-factors-for-fast-corneal-sensitivity-recovery-after-pterygium-excision
#2
Gemma Julio, Pamela Campos, Pere Pujol, Aitana Munguia, Enric Mas-Aixala
PURPOSE: To establish determining factors for fast corneal sensitivity (CS) recovery after pterygium excision. METHODS: Thirty-two eyes of 14 males and 18 females with primary nasal pterygium were recruited. Differences in CS (in the 4 quadrants and the center using a Cochet-Bonnet esthesiometer), pterygium corneal area (PCA), tear osmolarity, tear break-up time, Schirmer test, and ocular symptoms were analyzed before and 1 month after lesion excision. The relationship between CS recovery (difference between the 2 time points; CS1 - CS0) and the other features was assessed...
December 2016: Cornea
https://www.readbyqxmd.com/read/27006143/trh-injected-into-the-nucleus-accumbens-shell-releases-dopamine-and-reduces-feeding-motivation-in-rats
#3
L Puga, V Alcántara-Alonso, U Coffeen, O Jaimes, P de Gortari
The thyrotropin-releasing hormone (TRH), an anorexigenic factor that reduces food intake in food-restricted animals, may be involved in motivation for food. Injected centrally, TRH impairs acquisition of food-rewarded behavior. Through the TRH-R1 receptors, TRH injected in the nucleus accumbens increases dopamine content-perhaps the mechanism by which the peptide modulates food motivation. This, however, is still to be demonstrated. We sought to evaluate dopamine release by microdialysis after a TRH injection into the nucleus accumbens shell in free-moving fasted rats...
June 1, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/26818217/automated-agitation-assisted-demulsification-dispersive-liquid-liquid-microextraction
#4
Liang Guo, Shao Hua Chia, Hian Kee Lee
Dispersive liquid-liquid microextraction (DLLME) is an extremely fast and efficient sample preparation procedure. For its capability and applicability to be fully exploited, full automation of its operations seamlessly integrated with analysis is necessary. In this work, for the first time, fully automated agitation-assisted demulsification (AAD)-DLLME integrated with gas chromatography/mass spectrometry was developed for the convenient and efficient determination of polycyclic aromatic hydrocarbons in environmental water samples...
March 1, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/26496758/development-and-application-of-dual-fluorescence-reporter-systems-for-measuring-specific-nuclease-activity-based-on-ssa-repair-mechanism
#5
Fu-rong Han, Ling Wang, Kun Xu, Zhi-ying Zhang, Xin Wang
Reporter vector system has become an important method for measuring activity of specific nucleases because of its fast construction, simple modification, easy operation, economic effectiveness as well as its role in enriching positive cells with genomic modification through mediating screen of specific nucleases positive cells. After introducing double strand breaks (DSBs), a reporter system based on non-homologous end joining (NHEJ)-mediated repair can only repair maximally two thirds of reporter genes after optimization, while single strand annealing (SSA)-mediated repair can repair all reporter genes theoretically which has higher sensitivity and facilitates the detection of specific nuclease with low activity and provides an effective way to detect specific nuclease activity in genome modification studies...
October 2015: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/24772140/finite-element-analysis-of-a-bone-healing-model-1-year-follow-up-after-internal-fixation-surgery-for-femoral-fracture
#6
Zhou Jiang-Jun, Zhao Min, Yan Ya-Bo, Lei Wei, Lv Ren-Fa, Zhu Zhi-Yu, Chen Rong-Jian, Yu Wei-Tao, Du Cheng-Fei
Finite element analysis was used to compare preoperative and postoperative stress distribution of a bone healing model of femur fracture, to identify whether broken ends of fractured bone would break or not after fixation dislodgement one year after intramedullary nailing. Method s: Using fast, personalized imaging, bone healing models of femur fracture were constructed based on data from multi-slice spiral computed tomography using Mimics, Geomagic Studio, and Abaqus software packages. The intramedullary pin was removed by Boolean operations before fixation was dislodged...
March 2014: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/24491256/markov-mean-properties-for-cell-death-related-protein-classification
#7
Carlos Fernandez-Lozano, Marcos Gestal, Humberto González-Díaz, Julián Dorado, Alejandro Pazos, Cristian R Munteanu
The cell death (CD) is a dynamic biological function involved in physiological and pathological processes. Due to the complexity of CD, there is a demand for fast theoretical methods that can help to find new CD molecular targets. The current work presents the first classification model to predict CD-related proteins based on Markov Mean Properties. These protein descriptors have been calculated with the MInD-Prot tool using the topological information of the amino acid contact networks of the 2423 protein chains, five atom physicochemical properties and the protein 3D regions...
May 21, 2014: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/22903248/work-breaks-during-minimally-invasive-surgery-in-children-patient-benefits-and-surgeon-s-perceptions
#8
RANDOMIZED CONTROLLED TRIAL
Carsten Engelmann, Mischa Schneider, Gudela Grote, Clemens Kirschbaum, Jens Dingemann, Alexander Osthaus, Benno Ure
INTRODUCTION: We recently reported that 5-minute work breaks every 25 minutes during long lasting laparoscopy in children (intermittent pneumoperitoneum [IPP] scheme) decrease the surgeon's stress markers such as saliva cortisol and heart rate and improve time-concentration scores significantly. Data on the impact of breaks on the patient and on the surgeon's perception of breaks, however, are still lacking. MATERIALS AND METHODS: We present the comprehensive biometry data of a randomized trial including 26 patients operated with (IPP) and 26 patients without breaks (continuous pneumoperitoneum [CPP])...
December 2012: European Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/20120708/-assessment-of-the-selected-late-phase-of-angiogenesis-factors-concentration-in-colorectal-cancer-patients
#9
COMPARATIVE STUDY
Maria Wieloch, Magdalena Swiatkowska, Michał Libiszewski, Masoud Hedayati, Rafał Drozda, Krzysztof Kołomecki
UNLABELLED: Angiogenesis is a process of new blood vessels creating based on existing already vascularisation. This composed and multistage mechanism, which is responsible for growth of the tissues and organs, plays a crucial role in neoplasia. The exact role of particular cells, cytokines and extracellular matrix in cancerogenesis is still discussing. An identification of it is the key to searching the antiangiogenic substances breaking simultaneously neoplastic proliferation. THE AIM OF THE STUDY was to compare the angiopoietin-1(Ang-1), angiopoietin-2 (Ang-2) and Tie-2 receptor (rec...
December 2009: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/15162010/mechanisms-of-dna-double-strand-break-repair-and-chromosome-aberration-formation
#10
REVIEW
G Iliakis, H Wang, A R Perrault, W Boecker, B Rosidi, F Windhofer, W Wu, J Guan, G Terzoudi, G Pantelias
It is widely accepted that unrepaired or misrepaired DNA double strand breaks (DSBs) lead to the formation of chromosome aberrations. DSBs induced in the DNA of higher eukaryotes by endogenous processes or exogenous agents can in principle be repaired either by non-homologous endjoining (NHEJ), or homology directed repair (HDR). The basis on which the selection of the DSB repair pathway is made remains unknown but may depend on the inducing agent, or process. Evaluation of the relative contribution of NHEJ and HDR specifically to the repair of ionizing radiation (IR) induced DSBs is important for our understanding of the mechanisms leading to chromosome aberration formation...
2004: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/11402316/efficient-rejoining-of-radiation-induced-dna-double-strand-breaks-in-vertebrate-cells-deficient-in-genes-of-the-rad52-epistasis-group
#11
H Wang, Z C Zeng, T A Bui, E Sonoda, M Takata, S Takeda, G Iliakis
Rejoining of ionizing radiation (IR) induced DNA DSBs usually follows biphasic kinetics with a fast (t(50): 5-30 min) component attributed to DNA-PK-dependent non-homologous endjoining (NHEJ) and a slow (t(50): 1-20 h), as of yet uncharacterized, component. To examine whether homologous recombination (HR) contributes to DNA DSB rejoining, a systematic genetic study was undertaken using the hyper-recombinogenic DT40 chicken cell line and a series of mutants defective in HR. We show that DT40 cells rejoin IR-induced DNA DSBs with half times of 13 min and 4...
April 26, 2001: Oncogene
https://www.readbyqxmd.com/read/10728683/dna-dependent-protein-kinase-stimulates-an-independently-active-nonhomologous-end-joining-apparatus
#12
COMPARATIVE STUDY
S J DiBiase, Z C Zeng, R Chen, T Hyslop, W J Curran, G Iliakis
Double-strand breaks (DSBs) can be efficiently removed from the DNA of higher eukaryotes by nonhomologous end-joining (NHEJ). Genetic studies implicate the DNA-dependent protein kinase (DNA-PK) in NHEJ, but the exact function of this protein complex in the rejoining reaction remains to be elucidated. We compared rejoining of DNA DSBs in a human glioma cell line, M059-J, lacking the catalytic subunit of DNA-PK (DNA-PKcs), and their isogenic but DNA-PK-proficient counterpart, M059-K. In both cell lines, rejoining of DNA DSBs was biphasic, with a fast and a slow component operating with a half-life of approximately 22 min and 12 h, respectively...
March 1, 2000: Cancer Research
https://www.readbyqxmd.com/read/8352751/kinetics-and-mechanism-of-dna-repair-an-automated-programmable-apparatus-for-fast-time-resolved-studies-of-the-repair-of-mammalian-dna-after-u-v-irradiation
#13
C W Wharton, R A Meldrum, C Reason, J Boone, W Lester
An automated apparatus, designed and constructed for use in fast time-resolved studies of mammalian DNA repair after u.v. irradiation, is described. The methodology makes use of caged DNA-break-trapping reagents, e.g. [alpha-32P]dideoxyadenosine triphosphate [see Meldrum, Shall, Trentham and Wharton (1990) Biochem J. 266, 885-890] and the apparatus incorporates excimer lasers both for the delivery of u.v. damage and for the photoactivation of the caged reagents. The design is based on a sample-changing turntable which permits the sequential irradiation and quenching of samples...
August 1, 1993: Biochemical Journal
https://www.readbyqxmd.com/read/3370746/the-production-and-repair-of-dna-damage-by-n-nitrosobis-2-oxopropyl-amine-and-azaserine-in-hamster-and-rat-pancreas-acinar-and-duct-cells
#14
T Lawson, D Nagel
The activation of N-nitrosobis(2-oxopropyl)amine (BOP) by pancreas acinar and duct tissue from Syrian hamsters and MRC-Wistar rats in vitro and in vivo was measured in terms of the production and repair of DNA damage. Hamsters were given BOP (1 X 10 mg/kg, s.c.). DNA single strand breaks (SSB) were measured over 2 weeks. Significantly more SSB were present in duct than in acinar tissue. Their persistence in the duct fragments was due to a slower rate of repair. In a related experiment, duct fragments were isolated from BOP-treated (1 X 10 mg/kg, s...
June 1988: Carcinogenesis
https://www.readbyqxmd.com/read/1191177/the-radiobiology-of-dna-strand-breakage
#15
I Johansen
The yield of single-strand breaks in lambda DNA within lysogenic host bacteria was measured after exposure to 4-MeV electrons (50 msec) and rapid transfer (45 msec) to alkaline detergent. In nitrogen anoxia the yield was 1.2 X 10(-12) DNA single-strand breaks per rad per dalton, and under full oxygenation the yield increased to 5 X 10(-12) breaks per rad per dalton. A search for the presence of fast repair of strand breaks operating within a fraction of a second. Strand breaks produced in the persence of oxygen were repaired in 30-40 sec, while breaks produced under anoxia were rejoined even slower...
1975: Basic Life Sciences
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